Standard name
Human Ortholog
Description tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0.08 0 0 0 0 0 0
Cytoplasm 0.98 0.95 0.99 0.98 0.97 0.97 0.91 – 0.78 0.85 0.79 0.81 0.94 0.98 0.98 0.94 0.92 0.95 0.95 0.85 0.95 0.91 0.8 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0.07 – 0 0 0 0.05 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0.25 0.1 0.27 0.18 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0.05 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.11 0.09 0.06 0 0.06 0 0 0.14 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 – 0 1 1 3 0 0 0 0 0 0 1 1 1 0 0 0
Bud 0 0 0 0 0 1 1 – 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 6 9 2 8 7 8 – 0 4 1 7 1 2 6 7 6 10 0 0 0 0 0 0
Cytoplasm 271 159 227 222 251 250 269 – 72 192 168 196 67 188 159 144 160 125 255 158 225 23 24 73
Endoplasmic Reticulum 1 2 4 2 6 5 20 – 2 6 3 13 0 0 2 5 8 2 0 1 2 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 3 1 0 0 1
Golgi 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 1 0 0 2 0 0 0 0 0
Mitochondria 3 0 0 0 0 4 6 – 23 22 57 43 3 0 1 1 0 1 1 0 0 0 0 0
Nucleus 2 9 8 0 2 2 4 – 0 1 2 3 2 4 2 6 4 5 0 2 1 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 1 0 1 0 0 0 0 0 0 0 1 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 2 2 1 1 – 2 7 7 2 0 1 0 17 15 8 1 11 0 0 4 7
Unique Cell Count 276 167 230 226 259 259 296 92 226 214 241 71 191 163 153 173 132 268 186 238 26 31 88
Labelled Cell Count 278 177 248 228 270 270 309 101 234 241 267 73 195 170 181 193 151 268 186 238 26 31 88


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.1 14.0 11.5 11.0 10.5 8.7 8.1 – 6.2 7.4 6.3 6.4 10.6 11.7 11.7 16.5 17.2 16.7 – – 10.5
Std Deviation (1e-4) 1.6 2.6 1.6 1.6 1.4 1.2 1.2 – 0.9 1.3 1.1 1.0 1.8 1.7 2.0 3.5 3.3 4.1 – – 2.1
Intensity Change (Log2) – – – -0.06 -0.12 -0.41 -0.5 – -0.89 -0.64 -0.86 -0.84 -0.12 0.03 0.02 0.52 0.58 0.54 – – -0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.1 -0.5 -0.8 -0.8 – 0 -1.4 0 -0.6 0 0 -0.1 0.3 -0.2 1.5
Cytoplasm -0.4 -1.3 -1.5 -3.8 – -6.4 -5.4 -6.8 -6.2 -2.1 -0.2 -0.8 -2.5 -3.2 -2.2
Endoplasmic Reticulum 0 0 0 2.7 – 0 0 0 2.1 0 0 0 0 1.7 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 7.9 4.9 8.4 6.7 0 0 0 0 0 0
Nucleus 0 0 0 -1.6 – 0 0 0 -1.6 0 -0.8 0 0.2 -0.7 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 5.2 4.6 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.6274 7.5253 6.4627 6.2946 5.9646 6.8061 5.396 7.3482 5.9848 5.9363 5.4735 6.4266 4.7689 6.4805 6.2848 5.3892 6.0971 6.0628
Actin 0.0056 0.0002 0.0004 0.0007 0.0001 0.0019 0.0183 0.0012 0.012 0.002 0.0064 0.0023 0.0001 0.0002 0.0002 0.0002 0.0001 0.0005
Bud 0.0001 0.0001 0.0002 0.0001 0.0001 0.0002 0.0017 0.0019 0.0015 0.0003 0.0007 0.001 0 0.0001 0.0001 0.0001 0.0001 0.0001
Bud Neck 0.0005 0.0001 0.0003 0.0004 0.0004 0.0007 0.001 0.0002 0.0013 0.0004 0.0008 0.0007 0.0001 0.0001 0.0002 0.0002 0.0002 0.0006
Bud Periphery 0.0001 0 0 0.0001 0 0.0001 0.0017 0.0005 0.0011 0.0001 0.0009 0.0004 0 0 0 0 0 0
Bud Site 0.0004 0.0002 0.0002 0.0005 0.0001 0.0001 0.0048 0.0117 0.0027 0.0003 0.0007 0.0002 0.0001 0.0002 0.0001 0 0 0
Cell Periphery 0.0001 0 0 0 0 0.0001 0.0006 0.0001 0.0002 0 0.0002 0 0 0 0 0 0 0
Cytoplasm 0.9573 0.9768 0.9776 0.9397 0.9594 0.9644 0.8441 0.973 0.8986 0.8696 0.8626 0.949 0.9661 0.9894 0.9879 0.9902 0.9894 0.984
Cytoplasmic Foci 0.0058 0.0025 0.004 0.0067 0.0079 0.0058 0.0212 0.0025 0.0126 0.0158 0.0175 0.0079 0.0031 0.0017 0.0019 0.0022 0.003 0.0026
Eisosomes 0 0 0 0 0 0 0.0003 0 0.0004 0.0001 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0047 0.0013 0.0032 0.0022 0.0021 0.003 0.0079 0.0017 0.003 0.0082 0.0084 0.0025 0.0153 0.0027 0.0045 0.0019 0.0013 0.0059
Endosome 0.0073 0.003 0.0064 0.0135 0.0188 0.0073 0.0273 0.0011 0.0175 0.0356 0.0301 0.0127 0.0035 0.0014 0.0014 0.0017 0.0035 0.0022
Golgi 0.0013 0.0002 0.0004 0.0005 0.0004 0.0013 0.0106 0.0004 0.01 0.0037 0.0157 0.0049 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001
Lipid Particles 0.0005 0 0 0.0012 0 0.0003 0.0085 0 0.0016 0.0035 0.0045 0.0012 0 0 0 0 0 0
Mitochondria 0.0006 0.0001 0.0003 0.0008 0.0006 0.0011 0.0152 0.0002 0.014 0.001 0.0248 0.0098 0.0001 0 0.0001 0 0.0001 0.0001
None 0.0089 0.0053 0.0029 0.0087 0.0018 0.0059 0.0171 0.0027 0.0121 0.0526 0.0124 0.0017 0.0082 0.0026 0.0018 0.0013 0.0006 0.0013
Nuclear Periphery 0.0014 0.0004 0.0008 0.0037 0.0008 0.0036 0.0065 0.0004 0.0012 0.0013 0.0036 0.0011 0.001 0.0004 0.0006 0.0004 0.0003 0.0007
Nucleolus 0.0001 0 0 0.0012 0 0.0001 0.0003 0 0.0001 0.0001 0.0001 0 0 0 0 0 0 0
Nucleus 0.0024 0.0089 0.0017 0.0065 0.0022 0.0021 0.002 0.0014 0.0019 0.0013 0.0022 0.0012 0.0012 0.0006 0.0007 0.0006 0.0006 0.0012
Peroxisomes 0.0002 0 0 0.0005 0.0001 0.0001 0.0063 0.0001 0.0048 0.0006 0.0049 0.0016 0 0 0 0 0 0
Punctate Nuclear 0.0007 0.0001 0.0002 0.0082 0.0002 0.0003 0.0013 0.0003 0.0006 0.0016 0.0007 0.0006 0.0001 0 0 0.0001 0 0.0001
Vacuole 0.0017 0.0006 0.0012 0.004 0.0041 0.0011 0.0023 0.0003 0.002 0.0016 0.0015 0.0008 0.0006 0.0003 0.0004 0.0008 0.0005 0.0005
Vacuole Periphery 0.0003 0.0001 0.0003 0.0005 0.0009 0.0006 0.0011 0.0001 0.0006 0.0005 0.0011 0.0003 0.0001 0.0001 0.0001 0.0002 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 33.3482 22.5755 21.9065 21.6413 20.989 18.121 22.0945 25.6768 18.6612 25.5099
Translational Efficiency 1.0968 1.6046 1.4675 1.1238 1.2443 1.6867 1.6131 0.9679 1.2128 1.1586

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
101 1067 767 912 2484 1680 213 1046 2585 2747 980 1958

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 837.48 1109.72 1432.34 1218.68 1133.85 1079.91 1392.94 1262.95 1122.27 1091.49 1423.78 1242.33
Standard Deviation 119.13 194.71 171.31 220.77 148.87 165.48 148.75 174.26 158.58 178.00 167.46 198.52
Intensity Change Log 2 — 0.406069 0.774247 0.541193 — -0.070319 0.296903 0.155568 — 0.151518 0.519225 0.332119

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000577 0.000568 0.000342 0.001613 0.000075 0.000404 0.000779 0.001157 0.000095 0.000468 0.000437 0.001369
Bud Neck 0.007902 0.000726 0.001452 0.004409 0.000724 0.001203 0.001945 0.002268 0.001005 0.001018 0.001559 0.003265
Bud Site 0.039378 0.010124 0.006140 0.030510 0.004192 0.007682 0.012346 0.028075 0.005567 0.008631 0.007489 0.029209
Cell Periphery 0.000367 0.000086 0.000106 0.000178 0.000058 0.000049 0.000196 0.000088 0.000070 0.000063 0.000125 0.000130
Cytoplasm 0.597888* 0.827792* 0.816856* 0.638726* 0.852861* 0.790754* 0.728177* 0.766778* 0.842899* 0.805140* 0.797582* 0.707134*
Cytoplasmic Foci 0.231802* 0.072921 0.026642 0.036799 0.064045 0.073092 0.054092 0.038233 0.070600 0.073026 0.032609 0.037565
Eisosomes 0.000084 0.000010 0.000006 0.000025 0.000006 0.000011 0.000018 0.000010 0.000009 0.000010 0.000008 0.000017
Endoplasmic Reticulum 0.013379 0.000750 0.000576 0.001496 0.001215 0.000654 0.001069 0.000480 0.001690 0.000691 0.000683 0.000953
Endosome 0.013339 0.004890 0.002958 0.007593 0.002614 0.007167 0.006985 0.010502 0.003033 0.006282 0.003834 0.009147
Golgi 0.004816 0.002538 0.000520 0.003662 0.001895 0.004776 0.000827 0.008740 0.002009 0.003907 0.000586 0.006375
Lipid Particles 0.003444 0.001206 0.001194 0.001494 0.000612 0.000680 0.002660 0.001001 0.000723 0.000884 0.001513 0.001230
Mitochondria 0.001141 0.001360 0.000077 0.002131 0.000481 0.001573 0.000031 0.003163 0.000507 0.001490 0.000067 0.002682
Mitotic Spindle 0.004714 0.001700 0.007998 0.018594 0.001500 0.009914 0.004277 0.014537 0.001626 0.006723 0.007189 0.016427
None 0.000965 0.002629 0.002620 0.003157 0.003958 0.003052 0.000905 0.002007 0.003841 0.002888 0.002247 0.002542
Nuclear Periphery 0.000129 0.000080 0.000333 0.000493 0.000044 0.000098 0.000415 0.001375 0.000048 0.000091 0.000351 0.000964
Nuclear Periphery Foci 0.001219 0.003138 0.004398 0.004917 0.000860 0.001466 0.012976 0.004162 0.000874 0.002116 0.006262 0.004514
Nucleolus 0.000147 0.000195 0.000119 0.004618 0.000190 0.000217 0.000101 0.000229 0.000188 0.000208 0.000115 0.002274
Nucleus 0.021259 0.026600 0.074048 0.049956 0.027870 0.034526 0.033393 0.024853 0.027612 0.031447 0.065212 0.036546
Peroxisomes 0.001862 0.001410 0.000137 0.001553 0.000254 0.001093 0.000320 0.002366 0.000317 0.001216 0.000177 0.001987
Vacuole 0.052043 0.040418 0.053203 0.183464 0.035569 0.059926 0.138117 0.085203 0.036213 0.052349 0.071659 0.130971
Vacuole Periphery 0.003544 0.000860 0.000273 0.004611 0.000978 0.001662 0.000371 0.004775 0.001078 0.001351 0.000295 0.004699

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.03 0.60 -1.06 -1.10 -1.51 -3.35 -5.00 -3.86 -1.10 0.32 -3.95 -10.71 -3.64 -1.53 -1.53
Bud Neck 4.01 3.10 2.57 -4.61 -1.48 -3.68 -7.35 -6.76 -3.14 1.26 -0.40 -8.79 -5.49 -5.32 -0.55
Bud Site 3.28 3.56 2.16 -3.78 -4.96 -4.00 -6.84 -6.58 -4.47 -1.69 -3.82 -4.35 -7.92 -5.93 -5.90
Cell Periphery 4.28 3.48 3.54 -3.36 0.39 2.35 -5.58 -1.93 -3.78 5.08 1.34 -8.97 -3.90 -5.09 5.05
Cytoplasm -8.65 -5.93 -0.68 18.38 11.47 10.67 11.37 18.93 9.44 -2.32 8.40 14.49 26.57 19.00 7.84
Cytoplasmic Foci 8.88 11.39 11.23 11.07 -1.25 -2.31 2.56 11.28 11.88 4.28 -0.54 12.96 14.71 16.21 0.76
Eisosomes 5.64 5.77 5.00 -5.28 -6.66 -6.65 -8.75 -6.77 1.00 5.77 -2.33 -2.77 -5.39 -3.57 -3.11
Endoplasmic Reticulum 2.02 2.02 2.02 -0.05 -0.21 1.54 0.64 2.54 4.15 5.73 2.33 2.11 2.74 2.08 3.19
Endosome 3.41 3.93 3.74 1.05 -0.31 -6.26 -4.46 -1.90 2.66 1.85 -5.90 -3.12 -1.73 2.97 0.29
Golgi 2.08 3.99 2.62 1.01 -1.34 -2.96 8.78 -0.87 1.41 -2.25 -2.87 13.14 -0.23 1.87 -2.64
Lipid Particles 2.80 2.43 2.70 -0.83 1.09 -0.98 -5.57 -6.26 -4.63 4.12 -0.96 -9.11 -7.23 -2.53 4.30
Mitochondria -0.12 3.16 0.48 0.41 -1.57 -2.13 3.19 -1.05 0.74 -2.07 -2.01 3.13 -1.17 0.85 -2.52
Mitotic Spindle 0.66 -0.95 -1.21 -3.87 -0.56 -4.34 -2.45 -4.26 0.63 -1.66 -4.01 -4.91 -5.45 -1.49 -0.69
None -2.24 -2.80 -1.43 0.95 1.10 1.38 8.35 5.07 1.78 -3.87 1.88 4.66 4.73 1.96 0.90
Nuclear Periphery 0.74 -6.63 -3.74 -6.39 2.52 -8.77 -6.27 -1.72 -1.50 -0.28 -8.59 -16.47 -2.65 -2.32 0.18
Nuclear Periphery Foci -5.17 -9.60 -3.64 -0.48 3.49 -4.06 -8.98 -5.94 -4.00 7.13 -8.85 -15.07 -7.26 -3.15 7.74
Nucleolus -0.70 -0.20 -2.26 -2.20 -2.25 -1.15 6.21 -0.58 0.20 -2.31 -0.86 4.45 -2.24 -2.17 -2.37
Nucleus -1.68 -12.18 -5.83 -6.49 8.47 -5.25 -2.69 -2.97 1.26 0.70 -4.38 -15.95 -7.66 -3.80 10.46
Peroxisomes 0.83 5.11 2.60 0.82 -4.37 -4.06 -1.58 -1.72 0.33 -1.57 -3.72 4.39 -2.60 0.81 -3.09
Vacuole 0.65 -4.06 -15.44 -22.66 -15.01 -10.06 -11.61 -23.45 -17.63 1.32 -9.34 -14.83 -33.22 -28.02 -12.43
Vacuole Periphery 2.63 3.18 1.32 -1.27 -1.97 -1.09 4.79 -0.74 0.07 -1.78 -0.53 5.70 -1.07 -0.65 -2.65
Standard name
Human Ortholog
Description tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Trm44

Trm44


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Trm44-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available