Standard name
Human Ortholog
Description Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase); cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis; human ACAT1 functionally complements the growth defect caused by repression of ERG10 expression
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0 0.07 0.05 0.06 0.09 0.12 0.07 0.11 0.06 0.08 0.08 0.11 0.05 0 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.14 0.11 0.21 0.23 0.32 0.27 0 0 0 0.17 0.13 0.06 0 0 0 0 0 0
Nucleus 0.97 0.93 0.95 0.9 0.84 0.81 0.74 0.69 0.67 0.64 0.6 0.6 0.92 0.85 0.83 0.86 0.84 0.83 0.97 0.91 0.92 0.94 0.82 0.63
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.16 0.21 0.08 0.2 0.21 0.33 0.4 0.39 0.44 0.48 0.46 0.5 0.07 0.1 0.11 0.11 0.14 0.1 0 0 0 0 0.05 0.08
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1 2 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Neck 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1 0 0 1 0 0 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 3 18 13 37 19 49 70 57 55 53 35 13 17 25 11 6 12 0 0 0 0 0 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 4 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2 1 3 5 9 36 105 92 102 185 160 159 0 0 3 40 31 12 0 1 1 0 0 0
Nucleus 106 75 316 299 464 336 577 555 332 501 298 361 146 173 187 203 196 168 106 85 303 17 126 115
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 17 17 27 66 118 136 309 313 219 378 229 298 11 21 25 27 32 21 2 2 12 0 7 15
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 0 0 1 0 0 4 2 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 3 2 1 3 14 0 12 6 3 0 2 4 2 7 5 0 3 10 0 17 47
Unique Cell Count 109 81 332 332 553 416 776 809 493 788 496 599 159 204 226 237 232 203 110 94 331 19 155 184
Labelled Cell Count 125 97 364 386 633 528 1046 1048 710 1131 748 858 170 217 246 284 273 222 110 94 331 19 155 184


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 50.1 50.5 51.3 47.0 49.2 46.9 48.2 51.8 52.3 49.8 52.4 49.1 54.8 58.3 56.0 70.0 68.0 65.7 – 54.9 62.8
Std Deviation (1e-4) 12.5 20.0 20.3 18.2 22.2 20.6 21.7 25.0 24.4 21.1 27.2 21.5 24.2 29.0 28.8 29.9 29.7 25.9 – 24.0 26.2
Intensity Change (Log2) – – – -0.13 -0.06 -0.13 -0.09 0.01 0.03 -0.04 0.03 -0.06 0.09 0.18 0.13 0.45 0.41 0.36 – 0.1 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.9 0.8 -0.5 0.6 1.9 3.0 1.0 2.7 0.3 1.2 1.3 2.5 -0.4 -1.6 0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.7 6.5 5.8 8.4 9.2 11.1 9.9 0 0 0 7.1 6.1 3.4
Nucleus -2.5 -5.0 -5.9 -8.0 -9.6 -9.6 -10.9 -11.3 -11.5 -1.5 -4.1 -4.8 -4.0 -4.3 -4.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 4.4 5.1 8.1 10.5 10.3 11.2 12.7 11.6 12.8 -0.5 0.9 1.2 1.3 2.2 0.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 45.48 53.5859 49.2696 46.9392 47.1964 48.8132 51.4461 67.069 59.0461 49.6085 53.9703 57.0907 60.0738 69.2033 61.9535 56.2704 61.5762 61.0377
Actin 0.006 0 0.0167 0 0.0015 0 0.001 0 0.0002 0.0007 0.0219 0.0031 0.0017 0 0.0101 0.0001 0 0.003
Bud 0.0007 0 0.0003 0 0 0.0001 0.0009 0 0 0.0001 0.0002 0.0004 0.0002 0 0.0002 0 0 0.0001
Bud Neck 0.0002 0 0.0022 0 0.0001 0.0001 0.0001 0 0 0 0.0001 0.0005 0.0001 0 0.0001 0 0 0.0001
Bud Periphery 0.0008 0 0.0004 0 0 0.0001 0.0005 0 0 0.0001 0.0003 0.0009 0.0002 0 0.0002 0 0 0.0001
Bud Site 0.0015 0 0.0028 0 0.0001 0.0001 0.0004 0 0 0.0001 0.0002 0.0002 0.0009 0 0.0005 0 0 0
Cell Periphery 0.0011 0 0.0001 0 0.0001 0.0002 0.0003 0 0 0.0003 0.0002 0.0007 0.0006 0 0 0 0 0.0005
Cytoplasm 0.0023 0 0.0014 0 0.0003 0.0001 0.0008 0 0.0004 0.0009 0.0011 0.0001 0.0012 0 0.0014 0.0008 0 0.0002
Cytoplasmic Foci 0.0205 0 0.0014 0 0.0335 0.0005 0.0001 0 0 0.0025 0.0004 0.0009 0.0116 0 0.0008 0.0001 0 0.0004
Eisosomes 0.0002 0 0.0001 0 0 0 0 0 0 0 0.0001 0 0.0003 0 0 0 0 0
Endoplasmic Reticulum 0.0017 0 0.0025 0 0.0002 0 0.0002 0 0.0002 0.0005 0.0006 0.0001 0.0008 0 0.0002 0.0002 0 0.0001
Endosome 0.0075 0 0.0064 0 0.0115 0 0.0005 0 0.0004 0.0029 0.0033 0.0013 0.0056 0 0.0024 0.0007 0 0.0013
Golgi 0.0022 0 0.002 0 0.0041 0 0 0 0 0.0001 0.0014 0.0024 0.0016 0 0.0004 0 0 0.0006
Lipid Particles 0.0096 0 0.001 0 0.0338 0.0002 0 0 0 0.0004 0.0006 0.0029 0.0047 0 0.0002 0 0 0.0005
Mitochondria 0.0112 0.0003 0.0014 0.0004 0.0023 0.0013 0.007 0.0006 0.0097 0.0147 0.0145 0.006 0.008 0.0003 0.0013 0.0004 0.0027 0.0009
None 0.0054 0 0.0006 0 0.0005 0.0001 0.0005 0 0.0001 0.0007 0.0005 0.0001 0.0023 0 0.0003 0.0001 0 0.0001
Nuclear Periphery 0.0172 0.0001 0.0093 0.0004 0.0006 0.0002 0.0138 0.0002 0.0054 0.0202 0.0225 0.0011 0.0253 0.0001 0.0024 0.0107 0.0001 0.0041
Nucleolus 0.0924 0.0148 0.0168 0.011 0.3105 0.0982 0.0376 0.0111 0.0323 0.0676 0.1585 0.0714 0.0764 0.011 0.0146 0.0132 0.0875 0.0536
Nucleus 0.7996 0.9846 0.932 0.9881 0.5758 0.884 0.9326 0.9879 0.9502 0.8836 0.7687 0.9049 0.8499 0.9885 0.9625 0.9712 0.9093 0.928
Peroxisomes 0.0025 0 0.0003 0 0.0166 0 0.0001 0 0 0.0002 0.0004 0.0007 0.0013 0 0.0003 0 0 0.0004
Punctate Nuclear 0.0115 0 0.001 0 0.0078 0.0142 0.0018 0 0.0002 0.0005 0.0016 0.0005 0.0041 0 0.0008 0.0021 0 0.0048
Vacuole 0.0023 0 0.0007 0 0.0002 0.0003 0.0007 0 0.0002 0.001 0.0008 0.0008 0.0011 0 0.0007 0.0001 0 0.0006
Vacuole Periphery 0.0036 0 0.0006 0 0.0004 0.0003 0.0011 0.0002 0.0005 0.0028 0.0022 0.0012 0.002 0.0001 0.0005 0.0002 0.0003 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 377.0196 327.968 368.107 338.5118 359.7446 396.1581 320.5684 318.8494 371.7675 329.6273
Translational Efficiency 1.6985 1.7838 1.5737 2.0939 1.6085 1.5942 1.6747 1.8864 1.6075 1.6735

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
827 268 258 762 668 1726 722 636 1495 1994 980 1398

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 3178.91 3608.18 5303.19 5234.92 2828.91 3153.56 4283.93 4572.73 3022.52 3214.66 4552.27 4933.67
Standard Deviation 841.40 1037.19 1115.33 1571.43 749.87 816.49 1058.69 1646.81 820.46 863.53 1163.94 1639.66
Intensity Change Log 2 — 0.182739 0.738328 0.719635 — 0.156735 0.598689 0.692809 — 0.170553 0.674256 0.707066

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000425 0.000307 0.000766 0.000953 0.000514 0.000505 0.000561 0.000737 0.000464 0.000479 0.000615 0.000855
Bud Neck 0.001860 0.000897 0.004249 0.004460 0.001623 0.001769 0.002803 0.003854 0.001754 0.001652 0.003184 0.004184
Bud Site 0.000242 0.000088 0.000499 0.000467 0.000307 0.000267 0.000264 0.000384 0.000271 0.000243 0.000326 0.000429
Cell Periphery 0.000085 0.000033 0.000020 0.000112 0.000107 0.000291 0.000057 0.000080 0.000094 0.000256 0.000047 0.000098
Cytoplasm 0.000115 0.000022 0.000018 0.000332 0.000058 0.000123 0.000045 0.000400 0.000090 0.000109 0.000038 0.000363
Cytoplasmic Foci 0.000312 0.000181 0.000744 0.001809 0.000793 0.000443 0.000260 0.000404 0.000527 0.000408 0.000387 0.001170
Eisosomes 0.000345 0.000239 0.000333 0.000107 0.000373 0.000462 0.000273 0.000116 0.000357 0.000432 0.000289 0.000111
Endoplasmic Reticulum 0.000133 0.000072 0.000122 0.000173 0.000116 0.000319 0.000166 0.000123 0.000126 0.000285 0.000154 0.000151
Endosome 0.000003 0.000001 0.000002 0.000425 0.000004 0.000009 0.000009 0.000004 0.000003 0.000008 0.000007 0.000234
Golgi 0.000014 0.000004 0.000023 0.001244 0.000012 0.000019 0.000050 0.000023 0.000013 0.000017 0.000043 0.000688
Lipid Particles 0.000002 0.000000 0.000003 0.000037 0.000001 0.000010 0.000016 0.000003 0.000002 0.000009 0.000013 0.000021
Mitochondria 0.000778 0.000547 0.001854 0.003110 0.001006 0.000749 0.001271 0.002079 0.000880 0.000722 0.001425 0.002641
Mitotic Spindle 0.000011 0.000001 0.000006 0.000087 0.000004 0.000316 0.000090 0.000078 0.000008 0.000274 0.000068 0.000083
None 0.000429 0.000475 0.000395 0.001062 0.000808 0.000668 0.000526 0.001136 0.000598 0.000642 0.000492 0.001096
Nuclear Periphery 0.000018 0.000005 0.000017 0.000225 0.000019 0.000034 0.000021 0.000065 0.000018 0.000030 0.000020 0.000152
Nuclear Periphery Foci 0.000007 0.000000 0.000000 0.000095 0.000002 0.000056 0.000114 0.000006 0.000005 0.000048 0.000084 0.000055
Nucleolus 0.007735 0.006422 0.015673 0.029591 0.022133 0.006957 0.011837 0.020441 0.014168 0.006885 0.012847 0.025428
Nucleus 0.983755* 0.988019* 0.963000* 0.951561* 0.965134* 0.985015* 0.978319* 0.965894* 0.975435* 0.985419* 0.974286* 0.958082*
Peroxisomes 0.003346 0.002426 0.011951 0.002878 0.006451 0.001131 0.002997 0.003681 0.004734 0.001305 0.005354 0.003244
Vacuole 0.000294 0.000193 0.000206 0.000669 0.000320 0.000698 0.000205 0.000281 0.000306 0.000630 0.000205 0.000493
Vacuole Periphery 0.000090 0.000066 0.000118 0.000602 0.000216 0.000158 0.000117 0.000210 0.000146 0.000146 0.000117 0.000424

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 4.25 -8.01 -6.11 -7.43 -1.95 -0.34 -1.46 -3.81 -3.38 -3.00 -0.96 -5.86 -7.20 -6.01 -4.26
Bud Neck 5.15 -6.91 -7.77 -10.38 -0.64 -1.31 -6.45 -5.20 -4.80 -2.95 0.31 -8.83 -8.85 -8.83 -3.97
Bud Site 5.60 -4.92 -2.68 -4.29 -0.02 -0.40 0.90 -1.92 -0.98 -2.47 -0.45 -2.30 -3.29 -1.88 -2.29
Cell Periphery 1.98 2.59 -0.96 -3.07 -3.67 -2.22 1.29 0.62 2.60 -0.85 -2.32 1.84 -0.34 2.21 -2.30
Cytoplasm 1.92 1.98 -2.25 -3.11 -3.14 -1.77 0.39 -1.90 -1.37 -1.94 -0.93 1.55 -2.88 -2.35 -3.35
Cytoplasmic Foci 1.14 -3.03 -2.20 -2.43 -1.60 0.34 2.20 1.46 0.48 -1.39 -0.08 0.98 -1.74 -1.32 -2.12
Eisosomes 1.74 0.13 5.95 2.79 10.97 -1.22 1.55 4.20 8.53 6.94 -1.50 1.73 7.01 9.04 10.33
Endoplasmic Reticulum 5.02 0.69 -1.02 -2.63 -1.23 -5.87 -0.99 -0.80 5.45 0.79 -5.41 -0.83 -1.21 3.80 0.16
Endosome 2.85 1.48 -1.36 -1.37 -1.37 -2.10 -0.72 -0.69 1.87 0.60 -2.13 -0.70 -1.37 -1.32 -1.34
Golgi 1.88 -1.96 -1.40 -1.41 -1.38 -1.72 -0.97 -3.56 0.30 0.68 -1.34 -1.03 -1.41 -1.38 -1.33
Lipid Particles 1.72 -0.72 -1.55 -1.62 -1.52 -2.07 -0.99 -1.87 1.83 0.89 -1.94 -0.98 -1.59 -0.79 -0.69
Mitochondria 3.86 -7.86 -9.14 -10.13 -4.37 2.85 -2.98 -6.65 -8.39 -5.13 2.49 -7.85 -11.21 -12.12 -7.49
Mitotic Spindle 1.42 0.54 -2.08 -2.32 -2.19 -1.97 -1.03 -1.68 1.65 0.15 -1.95 -0.98 -2.67 1.55 -0.30
None -0.44 0.32 -2.35 -2.14 -2.46 0.82 1.49 -1.38 -1.76 -2.17 -0.43 0.76 -2.73 -2.12 -2.78
Nuclear Periphery 2.60 0.08 -3.14 -3.34 -3.16 -1.79 -0.28 -4.28 -2.41 -4.79 -1.85 -0.39 -3.71 -3.26 -3.68
Nuclear Periphery Foci 1.53 1.49 -1.33 -1.40 -1.40 -1.92 -0.99 -1.83 1.81 0.95 -1.83 -0.95 -1.36 -0.26 0.05
Nucleolus 0.70 -3.45 -5.89 -5.97 -3.33 6.26 3.71 0.33 -4.79 -2.80 5.23 0.62 -4.41 -7.79 -4.84
Nucleus -1.37 5.26 6.37 6.99 1.96 -5.82 -3.54 0.19 5.14 3.27 -4.37 0.69 5.25 8.44 4.79
Peroxisomes 0.77 -4.35 0.42 -0.52 4.91 5.75 3.42 2.34 -3.59 -0.85 5.10 -0.86 1.85 -4.32 3.06
Vacuole 1.45 1.68 -1.07 -1.21 -1.19 -3.80 1.78 -0.09 3.82 -2.02 -3.97 2.30 -1.07 -0.18 -1.32
Vacuole Periphery 1.77 -2.35 -4.15 -4.34 -3.92 1.37 2.67 -0.04 -1.82 -3.69 -0.09 1.51 -4.01 -3.99 -4.49
Standard name
Human Ortholog
Description Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase); cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis; human ACAT1 functionally complements the growth defect caused by repression of ERG10 expression
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-1

Erg10

Erg10


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Erg10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available