Standard name | |
---|---|
Human Ortholog | |
Description | Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; required for vacuolar protein sorting; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p; human ortholog is implicated in progressive cerebello-cerebral atrophy type 2 (PCCA2) |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 14 | 2 | 1 | 0 | 2 | 1 | 5 | – | 0 | 1 | 0 | 26 | 8 | 8 | 1 | 4 | 2 | 5 | 10 | 9 | 6 | 18 | 18 |
Bud | 6 | 4 | 13 | 10 | 14 | 17 | 36 | 51 | – | 49 | 53 | 56 | 5 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | – | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 2 | 1 | 0 | 1 | 2 | 14 | 21 | 25 | – | 26 | 32 | 25 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 2 | 0 | 1 | 1 | 1 | 2 | – | 7 | 3 | 4 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 4 | 20 | 15 | 19 | 15 | 16 | 16 | – | 17 | 12 | 2 | 9 | 10 | 9 | 23 | 10 | 2 | 1 | 0 | 0 | 0 | 0 | 1 |
Endoplasmic Reticulum | 1 | 6 | 4 | 0 | 1 | 2 | 0 | 4 | – | 0 | 0 | 0 | 10 | 1 | 6 | 3 | 2 | 1 | 1 | 0 | 1 | 2 | 1 | 2 |
Endosome | 47 | 16 | 113 | 46 | 99 | 33 | 40 | 27 | – | 8 | 6 | 17 | 58 | 50 | 59 | 42 | 28 | 10 | 72 | 88 | 113 | 67 | 81 | 92 |
Golgi | 30 | 20 | 8 | 6 | 9 | 0 | 0 | 0 | – | 0 | 0 | 0 | 107 | 42 | 80 | 4 | 8 | 7 | 110 | 130 | 151 | 74 | 140 | 219 |
Mitochondria | 200 | 289 | 301 | 234 | 244 | 453 | 613 | 715 | – | 724 | 573 | 629 | 114 | 55 | 34 | 11 | 12 | 1 | 10 | 12 | 25 | 17 | 20 | 17 |
Nucleus | 1 | 0 | 3 | 1 | 1 | 1 | 2 | 8 | – | 6 | 2 | 2 | 0 | 1 | 1 | 6 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 4 | 1 | 1 | 1 | 3 | 4 | 4 | – | 6 | 3 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 5 | 16 | 9 | 12 | 6 | 33 | 63 | 72 | – | 55 | 35 | 39 | 2 | 3 | 4 | 4 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Peroxisomes | 41 | 22 | 193 | 71 | 92 | 66 | 126 | 129 | – | 79 | 62 | 102 | 33 | 41 | 7 | 11 | 6 | 3 | 97 | 68 | 173 | 154 | 220 | 204 |
SpindlePole | 20 | 11 | 58 | 39 | 47 | 43 | 64 | 82 | – | 69 | 56 | 67 | 45 | 13 | 37 | 29 | 16 | 3 | 6 | 8 | 13 | 8 | 3 | 3 |
Vac/Vac Membrane | 7 | 4 | 4 | 0 | 8 | 3 | 10 | 8 | – | 3 | 4 | 5 | 8 | 13 | 15 | 2 | 3 | 2 | 1 | 1 | 2 | 1 | 1 | 0 |
Unique Cell Count | 272 | 321 | 479 | 324 | 378 | 547 | 736 | 850 | 822 | 638 | 723 | 303 | 161 | 201 | 92 | 59 | 23 | 312 | 326 | 497 | 338 | 493 | 565 | |
Labelled Cell Count | 368 | 412 | 732 | 437 | 544 | 686 | 997 | 1149 | 1050 | 842 | 952 | 422 | 239 | 265 | 138 | 93 | 31 | 312 | 326 | 497 | 338 | 493 | 565 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.2 | 6.5 | 6.2 | 5.7 | 5.6 | 4.7 | 4.6 | 4.8 | – | 4.2 | 4.2 | 4.4 | 7.6 | 7.8 | 7.9 | 7.4 | 8.5 | 9.9 | 7.8 | 8.2 | 8.2 |
Std Deviation (1e-4) | 0.7 | 0.9 | 1.3 | 1.5 | 1.2 | 1.1 | 1.2 | 1.0 | – | 1.1 | 1.0 | 1.0 | 0.9 | 1.1 | 0.9 | 1.7 | 2.0 | 1.8 | 1.4 | 1.3 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.14 | -0.4 | -0.42 | -0.37 | – | -0.56 | -0.54 | -0.48 | 0.29 | 0.33 | 0.35 | 0.26 | 0.45 | 0.68 | 0.34 | 0.4 | 0.41 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 6.0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0.3 | 0.8 | 0 | 0 | 0 | – | 0 | 0 | 0 | -1.0 | 0 | -0.6 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.3 | 0.6 | -1.3 | -2.0 | -2.5 | – | -2.2 | -2.3 | -4.9 | -0.9 | 1.1 | 0.2 | 6.9 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2.5 | 0 | 0 | 0 | 0 | 0 |
Endosome | -3.3 | 0.9 | 0 | 0 | 0 | – | 0 | 0 | 0 | -1.5 | 1.9 | 1.6 | 4.4 | 3.9 | 2.2 |
Golgi | 0.2 | 0.7 | 0 | 0 | 0 | – | 0 | 0 | 0 | 12.9 | 10.0 | 13.5 | 0 | 0 | 0 |
Mitochondria | 2.8 | 0.5 | 0 | 0 | 0 | – | 0 | 0 | 0 | -6.9 | -6.3 | -10.9 | -9.0 | -6.2 | -5.6 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.6 | -0.3 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -5.4 | -4.9 | 0 | 0 | 0 | – | 0 | 0 | 0 | -8.8 | -3.4 | -9.6 | -5.2 | -4.5 | -2.6 |
SpindlePole | 0 | 0.1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1.1 | -1.4 | 2.2 | 4.7 | 3.2 | 0 |
Vacuole | 0 | 1.6 | 0 | 0.8 | 0 | – | 0 | 0 | 0 | 0 | 0 | 4.8 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.0776 | 3.6173 | 3.328 | 3.0208 | 2.8036 | 3.098 | 5.0385 | 4.8269 | 4.4131 | 3.998 | 5.2051 | 4.608 | 3.8772 | 5.1581 | 4.5858 | 4.9932 | 4.2495 | 4.7255 |
Actin | 0.0369 | 0.0108 | 0.0164 | 0.0064 | 0.0019 | 0.0163 | 0.0795 | 0.0156 | 0.0399 | 0.0044 | 0.0253 | 0.0184 | 0.0057 | 0.0029 | 0.0085 | 0.0026 | 0.0009 | 0.0086 |
Bud | 0.0005 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0008 | 0.0008 | 0.0009 | 0 | 0.0013 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0009 |
Bud Neck | 0.0017 | 0.0008 | 0.0009 | 0.0016 | 0.0005 | 0.0006 | 0.0037 | 0.0008 | 0.0012 | 0.0001 | 0.0006 | 0.0007 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0004 |
Bud Periphery | 0.0005 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.001 | 0.0005 | 0.0008 | 0 | 0.0019 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0003 |
Bud Site | 0.003 | 0.0055 | 0.0006 | 0.0013 | 0.0022 | 0.0007 | 0.016 | 0.0137 | 0.0057 | 0.0001 | 0.0024 | 0.0005 | 0.0004 | 0.0013 | 0.0003 | 0.0004 | 0.0001 | 0.0002 |
Cell Periphery | 0.0002 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 |
Cytoplasm | 0.0072 | 0.0034 | 0.0015 | 0.0042 | 0.002 | 0.0008 | 0.0064 | 0.011 | 0.0023 | 0.0007 | 0.0029 | 0.0021 | 0.0006 | 0.0029 | 0.0014 | 0.0005 | 0.0004 | 0.0042 |
Cytoplasmic Foci | 0.1499 | 0.2328 | 0.1981 | 0.1316 | 0.2346 | 0.1355 | 0.1474 | 0.2283 | 0.1806 | 0.1192 | 0.113 | 0.1683 | 0.1381 | 0.2275 | 0.1647 | 0.16 | 0.2179 | 0.1355 |
Eisosomes | 0.0003 | 0.0001 | 0.0001 | 0.0008 | 0 | 0 | 0.0008 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0043 | 0.0004 | 0.0009 | 0.0005 | 0.0001 | 0.0003 | 0.0048 | 0.0014 | 0.0009 | 0.0001 | 0.001 | 0.0005 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Endosome | 0.1652 | 0.2275 | 0.1692 | 0.1828 | 0.1719 | 0.1336 | 0.186 | 0.1923 | 0.1596 | 0.2535 | 0.1409 | 0.1219 | 0.1545 | 0.1894 | 0.1633 | 0.1001 | 0.1293 | 0.0977 |
Golgi | 0.1713 | 0.2591 | 0.2561 | 0.317 | 0.3977 | 0.3752 | 0.1026 | 0.2015 | 0.2163 | 0.1387 | 0.1203 | 0.2333 | 0.174 | 0.274 | 0.2584 | 0.1972 | 0.2707 | 0.2218 |
Lipid Particles | 0.1311 | 0.0548 | 0.0911 | 0.1392 | 0.0642 | 0.1226 | 0.1203 | 0.0714 | 0.1121 | 0.0386 | 0.2056 | 0.0847 | 0.1371 | 0.0439 | 0.0777 | 0.0834 | 0.0581 | 0.0847 |
Mitochondria | 0.013 | 0.0215 | 0.0208 | 0.0076 | 0.0089 | 0.0252 | 0.0192 | 0.0126 | 0.0136 | 0.0043 | 0.0073 | 0.0186 | 0.0134 | 0.0066 | 0.0165 | 0.0301 | 0.0064 | 0.0134 |
None | 0.0053 | 0.0002 | 0.0009 | 0.026 | 0.0002 | 0.0001 | 0.0085 | 0.0029 | 0.0005 | 0.0001 | 0.0022 | 0.0039 | 0.0003 | 0.0085 | 0.001 | 0.0001 | 0.0002 | 0.0002 |
Nuclear Periphery | 0.0251 | 0.0002 | 0.0013 | 0.0012 | 0.0001 | 0.0002 | 0.0279 | 0.0006 | 0.0007 | 0 | 0.0248 | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 |
Nucleolus | 0.0005 | 0.0003 | 0.0002 | 0.0053 | 0.0001 | 0.0014 | 0.001 | 0.0003 | 0.0001 | 0 | 0.0023 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 |
Nucleus | 0.0055 | 0.0002 | 0.0004 | 0.0053 | 0.0001 | 0.0002 | 0.0043 | 0.0003 | 0.0005 | 0 | 0.0576 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0003 |
Peroxisomes | 0.2685 | 0.173 | 0.238 | 0.1559 | 0.1132 | 0.182 | 0.2501 | 0.2278 | 0.2608 | 0.4376 | 0.2638 | 0.3432 | 0.3647 | 0.2384 | 0.3053 | 0.4227 | 0.3107 | 0.4298 |
Punctate Nuclear | 0.0047 | 0.0023 | 0.0015 | 0.0112 | 0.001 | 0.0028 | 0.0147 | 0.0065 | 0.0015 | 0.0003 | 0.0233 | 0.0012 | 0.0088 | 0.001 | 0.0004 | 0.0006 | 0.003 | 0.0004 |
Vacuole | 0.0029 | 0.0049 | 0.0008 | 0.0008 | 0.0006 | 0.0011 | 0.0023 | 0.0099 | 0.0012 | 0.0013 | 0.0018 | 0.0006 | 0.0011 | 0.0015 | 0.0009 | 0.0008 | 0.001 | 0.0008 |
Vacuole Periphery | 0.0024 | 0.0014 | 0.0008 | 0.0008 | 0.0006 | 0.0009 | 0.0021 | 0.0014 | 0.0007 | 0.0008 | 0.0014 | 0.0006 | 0.0006 | 0.0007 | 0.0006 | 0.0007 | 0.0007 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 25.6969 | 23.5708 | 18.6763 | 21.9823 | 20.28 | 24.1194 | 25.4481 | 27.2631 | 22.3429 | 25.9217 |
Translational Efficiency | 0.4983 | 0.5709 | 0.5804 | 0.4851 | 0.5298 | 0.4541 | 0.4794 | 0.4577 | 0.602 | 0.5423 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
267 | 792 | 1387 | 1016 | 2213 | 1028 | 1906 | 1740 | 2480 | 1820 | 3293 | 2756 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 958.26 | 863.01 | 1030.67 | 983.25 | 787.25 | 833.32 | 967.78 | 1011.83 | 805.66 | 846.24 | 994.27 | 1001.29 |
Standard Deviation | 139.48 | 119.93 | 126.45 | 130.57 | 93.73 | 102.40 | 111.22 | 127.61 | 112.89 | 111.35 | 121.90 | 129.45 |
Intensity Change Log 2 | — | -0.151040 | 0.105093 | 0.037141 | — | 0.082049 | 0.297857 | 0.362073 | — | -0.041232 | 0.195233 | 0.192798 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.011015 | 0.003508 | 0.018290 | 0.024235 | 0.002350 | 0.002864 | 0.003781 | 0.015203 | 0.003283 | 0.003144 | 0.009892 | 0.018533 |
Bud Neck | 0.002133 | 0.000550 | 0.000547 | 0.001090 | 0.000228 | 0.000207 | 0.000599 | 0.000512 | 0.000433 | 0.000356 | 0.000577 | 0.000725 |
Bud Site | 0.019223 | 0.000837 | 0.002040 | 0.009096 | 0.000759 | 0.000593 | 0.001661 | 0.005545 | 0.002747 | 0.000699 | 0.001821 | 0.006854 |
Cell Periphery | 0.000321 | 0.000039 | 0.000044 | 0.000059 | 0.000050 | 0.000033 | 0.000026 | 0.000049 | 0.000079 | 0.000035 | 0.000034 | 0.000052 |
Cytoplasm | 0.005639 | 0.000986 | 0.000997 | 0.002772 | 0.001331 | 0.000524 | 0.000935 | 0.003128 | 0.001795 | 0.000725 | 0.000961 | 0.002997 |
Cytoplasmic Foci | 0.295760* | 0.361907* | 0.482468* | 0.387593* | 0.216732* | 0.278747* | 0.469968* | 0.356264* | 0.225240* | 0.314935* | 0.475233* | 0.367813* |
Eisosomes | 0.000110 | 0.000047 | 0.000057 | 0.000058 | 0.000068 | 0.000051 | 0.000030 | 0.000053 | 0.000073 | 0.000049 | 0.000041 | 0.000055 |
Endoplasmic Reticulum | 0.001796 | 0.000220 | 0.000444 | 0.000230 | 0.000084 | 0.000249 | 0.000180 | 0.000182 | 0.000269 | 0.000236 | 0.000291 | 0.000200 |
Endosome | 0.292041* | 0.179572 | 0.176584 | 0.215837* | 0.187853 | 0.217606* | 0.215167* | 0.226659* | 0.199070 | 0.201055* | 0.198916 | 0.222670* |
Golgi | 0.166495* | 0.311314* | 0.202029* | 0.199412* | 0.451004* | 0.332230* | 0.156623* | 0.237450* | 0.420373* | 0.323128* | 0.175748* | 0.223427* |
Lipid Particles | 0.012846 | 0.025907 | 0.041909 | 0.009224 | 0.011671 | 0.023198 | 0.020506 | 0.003257 | 0.011797 | 0.024377 | 0.029521 | 0.005457 |
Mitochondria | 0.007879 | 0.014266 | 0.003170 | 0.004682 | 0.062683 | 0.025903 | 0.003891 | 0.012480 | 0.056783 | 0.020839 | 0.003587 | 0.009605 |
Mitotic Spindle | 0.046666 | 0.010717 | 0.035844 | 0.046127 | 0.004552 | 0.003046 | 0.055191 | 0.024712 | 0.009086 | 0.006384 | 0.047042 | 0.032607 |
None | 0.000049 | 0.000021 | 0.000013 | 0.000022 | 0.000117 | 0.000016 | 0.000010 | 0.000013 | 0.000110 | 0.000018 | 0.000011 | 0.000016 |
Nuclear Periphery | 0.000253 | 0.000134 | 0.000180 | 0.000080 | 0.000014 | 0.000015 | 0.000098 | 0.000043 | 0.000040 | 0.000066 | 0.000133 | 0.000057 |
Nuclear Periphery Foci | 0.001643 | 0.000447 | 0.001473 | 0.000801 | 0.000075 | 0.000094 | 0.001317 | 0.000260 | 0.000244 | 0.000248 | 0.001383 | 0.000460 |
Nucleolus | 0.000128 | 0.000176 | 0.000036 | 0.000057 | 0.000355 | 0.000054 | 0.000115 | 0.000016 | 0.000330 | 0.000107 | 0.000082 | 0.000032 |
Nucleus | 0.000249 | 0.000323 | 0.000040 | 0.000066 | 0.000156 | 0.000023 | 0.000037 | 0.000019 | 0.000166 | 0.000154 | 0.000038 | 0.000036 |
Peroxisomes | 0.111716* | 0.085984 | 0.028111 | 0.089094 | 0.057553 | 0.112517* | 0.064891 | 0.107289* | 0.063384 | 0.100970* | 0.049399 | 0.100581* |
Vacuole | 0.017324 | 0.001649 | 0.004583 | 0.008040 | 0.001165 | 0.000995 | 0.004234 | 0.005699 | 0.002904 | 0.001280 | 0.004381 | 0.006562 |
Vacuole Periphery | 0.006713 | 0.001398 | 0.001141 | 0.001423 | 0.001201 | 0.001037 | 0.000739 | 0.001167 | 0.001794 | 0.001194 | 0.000908 | 0.001261 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.49 | -2.05 | -3.12 | -6.45 | -1.56 | -0.86 | -2.26 | -8.26 | -7.79 | -7.15 | 0.21 | -6.24 | -9.76 | -9.89 | -4.86 |
Bud Neck | 3.77 | 3.83 | 2.36 | -3.25 | -3.65 | 0.50 | -3.74 | -5.31 | -5.39 | 0.83 | 1.15 | -1.74 | -3.78 | -4.83 | -1.83 |
Bud Site | 4.12 | 3.86 | 2.14 | -5.47 | -4.70 | 0.92 | -4.77 | -7.11 | -7.34 | -5.75 | 3.99 | 1.88 | -4.73 | -8.72 | -7.16 |
Cell Periphery | 2.05 | 2.01 | 1.89 | -3.12 | -2.36 | 2.66 | 4.69 | 0.08 | -2.22 | -3.71 | 2.74 | 2.86 | 1.59 | -3.45 | -4.16 |
Cytoplasm | 2.13 | 2.05 | 1.37 | -3.70 | -3.57 | 2.23 | 1.05 | -3.18 | -5.78 | -4.81 | 2.54 | 1.85 | -2.31 | -6.57 | -5.82 |
Cytoplasmic Foci | -5.02 | -14.44 | -6.90 | -2.64 | 10.86 | -10.29 | -42.29 | -24.54 | -10.99 | 16.21 | -17.17 | -50.81 | -28.81 | -9.13 | 19.68 |
Eisosomes | 1.36 | 1.13 | 1.10 | -2.80 | -0.24 | 4.00 | 11.80 | 4.07 | -0.45 | -8.83 | 3.89 | 5.31 | 2.98 | -2.05 | -5.53 |
Endoplasmic Reticulum | 2.94 | 2.51 | 2.93 | -0.19 | 5.15 | -0.86 | -3.76 | -1.99 | 0.33 | -0.05 | 0.25 | -0.43 | 0.98 | 0.30 | 2.59 |
Endosome | 7.67 | 8.07 | 5.19 | -4.50 | -5.32 | -4.52 | -4.97 | -6.84 | -1.27 | -1.90 | -0.39 | 0.02 | -4.72 | -4.05 | -5.06 |
Golgi | -12.64 | -3.51 | -3.14 | 12.97 | 0.43 | 14.74 | 50.60* | 32.92 | 12.23 | -14.91 | 14.46 | 44.48 | 33.79 | 17.23 | -10.94 |
Lipid Particles | -3.12 | -7.51 | 1.34 | 4.71 | 10.31 | -3.68 | -4.35 | 7.05 | 6.77 | 9.72 | -5.17 | -9.33 | 5.58 | 8.38 | 14.30 |
Mitochondria | -2.29 | 2.34 | 1.50 | 4.23 | -1.36 | 9.19 | 18.91 | 14.84 | 4.58 | -5.72 | 11.04 | 19.03 | 16.10 | 5.73 | -5.82 |
Mitotic Spindle | 4.16 | 1.23 | 0.05 | -7.00 | -1.89 | 1.83 | -14.57 | -9.22 | -9.92 | 7.60 | 1.84 | -14.38 | -9.75 | -11.01 | 4.44 |
None | 1.09 | 1.33 | 1.03 | -0.30 | -1.83 | 1.24 | 1.30 | 1.28 | 1.10 | -1.07 | 1.27 | 1.35 | 1.29 | 0.52 | -2.07 |
Nuclear Periphery | 1.17 | 0.93 | 2.29 | 0.71 | 3.80 | -0.11 | -8.15 | -3.49 | -3.28 | 4.16 | -0.83 | -7.01 | -1.58 | 0.28 | 5.72 |
Nuclear Periphery Foci | 2.35 | 0.17 | 1.54 | -1.21 | 2.04 | -0.49 | -4.47 | -2.65 | -2.29 | 3.75 | -0.00 | -5.81 | -1.96 | -1.69 | 4.39 |
Nucleolus | -0.47 | 1.82 | 1.32 | 1.32 | -0.45 | 1.10 | 0.82 | 1.27 | 0.86 | 1.49 | 0.92 | 0.99 | 1.25 | 1.66 | 1.32 |
Nucleus | -0.14 | 1.45 | 1.34 | 1.11 | -0.52 | 1.14 | 1.03 | 1.19 | 0.33 | 2.14 | 0.12 | 1.21 | 1.27 | 1.17 | 0.46 |
Peroxisomes | 2.00 | 7.10 | 1.78 | -0.40 | -10.68 | -8.52 | -1.73 | -9.34 | 0.71 | -7.56 | -7.54 | 3.96 | -8.37 | 0.07 | -12.55 |
Vacuole | 5.79 | 4.68 | 3.16 | -6.33 | -3.43 | 0.98 | -8.28 | -6.13 | -6.25 | -1.79 | 4.65 | -3.62 | -5.54 | -8.74 | -3.48 |
Vacuole Periphery | 2.73 | 2.97 | 2.78 | -0.08 | -1.06 | 0.54 | 3.72 | 0.20 | -0.43 | -3.36 | 1.67 | 3.82 | 2.03 | -0.25 | -2.72 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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