Standard name | |
---|---|
Human Ortholog | |
Description | Outer kinetochore protein, needed for accurate chromosome segregation; component of kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as platform for kinetochore assembly; required for spindle assembly checkpoint; minimizes potentially deleterious centromere-proximal crossovers by preventing meiotic DNA break formation proximal to centromere; homolog of human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 2 | 0 | 4 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud | 2 | 2 | 4 | 1 | 11 | 12 | 34 | 27 | 35 | 28 | 26 | 34 | 2 | 0 | 1 | 1 | 2 | 1 | 1 | 3 | 2 | 1 | 4 | 3 |
Bud Neck | 1 | 0 | 21 | 7 | 29 | 17 | 21 | 42 | 21 | 27 | 17 | 44 | 2 | 5 | 4 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 3 |
Bud Site | 7 | 1 | 7 | 5 | 14 | 19 | 39 | 41 | 37 | 33 | 43 | 42 | 2 | 1 | 0 | 2 | 5 | 6 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 8 | 7 | 23 | 30 | 36 | 30 | 31 | 31 | 21 | 23 | 20 | 23 | 1 | 24 | 33 | 26 | 28 | 19 | 0 | 2 | 8 | 1 | 4 | 12 |
Endoplasmic Reticulum | 1 | 0 | 0 | 1 | 1 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 5 | 3 | 0 | 1 | 1 | 0 | 0 | 1 |
Endosome | 11 | 57 | 5 | 28 | 14 | 24 | 15 | 3 | 7 | 2 | 2 | 8 | 1 | 7 | 28 | 90 | 142 | 132 | 17 | 29 | 22 | 6 | 13 | 16 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 2 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 58 | 25 | 19 | 6 | 9 | 55 | 104 | 60 | 125 | 105 | 142 | 133 | 5 | 1 | 1 | 18 | 13 | 12 | 1 | 5 | 2 | 0 | 5 | 4 |
Nucleus | 9 | 17 | 14 | 16 | 19 | 22 | 27 | 29 | 22 | 29 | 25 | 28 | 2 | 5 | 10 | 23 | 22 | 12 | 4 | 9 | 6 | 5 | 12 | 4 |
Nuclear Periphery | 0 | 4 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 82 | 112 | 39 | 61 | 88 | 77 | 156 | 140 | 128 | 111 | 119 | 125 | 13 | 18 | 24 | 100 | 137 | 96 | 8 | 18 | 6 | 1 | 12 | 5 |
Peroxisomes | 3 | 1 | 0 | 1 | 1 | 4 | 9 | 4 | 3 | 3 | 3 | 2 | 0 | 0 | 2 | 4 | 4 | 4 | 1 | 1 | 2 | 0 | 0 | 1 |
SpindlePole | 96 | 101 | 141 | 181 | 293 | 266 | 362 | 357 | 266 | 263 | 220 | 321 | 20 | 70 | 71 | 169 | 181 | 118 | 129 | 157 | 175 | 59 | 150 | 103 |
Vac/Vac Membrane | 3 | 7 | 0 | 2 | 0 | 1 | 2 | 3 | 0 | 1 | 1 | 2 | 3 | 1 | 2 | 15 | 29 | 31 | 5 | 3 | 6 | 2 | 5 | 6 |
Unique Cell Count | 163 | 200 | 189 | 245 | 361 | 358 | 514 | 471 | 417 | 405 | 356 | 479 | 35 | 109 | 133 | 258 | 323 | 251 | 176 | 240 | 241 | 85 | 217 | 173 |
Labelled Cell Count | 281 | 334 | 273 | 339 | 517 | 532 | 802 | 737 | 668 | 627 | 619 | 766 | 53 | 133 | 176 | 454 | 572 | 438 | 176 | 240 | 241 | 85 | 217 | 173 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.9 | 5.4 | 4.7 | 5.1 | 5.0 | 4.4 | 4.1 | 4.4 | 3.8 | 3.9 | 3.6 | 3.9 | 4.6 | 5.5 | 5.9 | 7.4 | 7.5 | 7.1 | 4.8 | 5.3 | 5.9 |
Std Deviation (1e-4) | 0.7 | 0.9 | 1.2 | 1.8 | 1.5 | 1.7 | 1.6 | 1.4 | 1.3 | 1.1 | 1.3 | 1.0 | 0.6 | 1.2 | 2.0 | 1.7 | 2.0 | 1.7 | 1.4 | 1.4 | 1.8 |
Intensity Change (Log2) | – | – | – | 0.12 | 0.09 | -0.11 | -0.21 | -0.1 | -0.32 | -0.26 | -0.39 | -0.28 | -0.03 | 0.21 | 0.33 | 0.65 | 0.67 | 0.59 | 0.03 | 0.17 | 0.32 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | 0 | 0.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Neck | -3.5 | -1.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | -1.9 | -2.7 | -5.5 | -6.1 | -5.4 |
Bud Site | -1.0 | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | -0.8 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Endosome | 3.4 | 0.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 5.4 | 8.2 | 10.0 | 11.2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -3.4 | -3.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | -3.0 | -3.4 | -1.2 | -2.7 | -2.1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | 1.0 | 1.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | -0.9 | -0.6 | 4.1 | 5.0 | 4.0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -0.2 | 1.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | -1.9 | -4.0 | -2.1 | -4.2 | -5.8 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | -0.8 | -1.4 | -2.7 | -2.4 | -3.1 | -2.8 | -2.7 | -3.4 | – | 2.2 | 2.9 | -0.7 | -1.3 | -1.6 |
Nucleus | -0.4 | -1.0 | -0.6 | -1.1 | -0.6 | -1.0 | -0.1 | -0.2 | -0.7 | – | -1.0 | 0 | 0.6 | -0.3 | -1.2 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 3.4 | 4.2 | 5.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.2513 | 0.4787 | 0.5068 | 0.1689 | 0.0105 | 0.4165 | 0.2976 | 0.4008 | 0.35 | 0.1184 | 0.1983 | 0.4637 | 0.0538 | -0.1094 | -0.123 | 0.1884 | -0.5183 | -0.2661 |
Actin | 0.0014 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0008 | 0.0053 | 0.0001 | 0.0004 | 0.0004 | 0.006 | 0.0014 | 0.0148 | 0.0003 | 0.0127 | 0.0026 | 0.0001 | 0.0006 |
Bud | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0025 | 0.0003 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 |
Bud Neck | 0.0005 | 0.0006 | 0.0003 | 0.0003 | 0.0003 | 0.0009 | 0.0012 | 0.0009 | 0.0036 | 0.001 | 0.0006 | 0.001 | 0.0023 | 0.001 | 0.0012 | 0.0036 | 0.0011 | 0.0052 |
Bud Periphery | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0014 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0002 |
Bud Site | 0.0003 | 0.0005 | 0.0007 | 0 | 0.0001 | 0.0001 | 0.0028 | 0.001 | 0.0005 | 0.0004 | 0.0003 | 0.0001 | 0.001 | 0.0013 | 0.0035 | 0.0004 | 0.0003 | 0.0004 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0.0006 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.0019 | 0.0026 | 0.0032 | 0.0012 | 0.0003 | 0.0016 | 0.0055 | 0.0162 | 0.0058 | 0.0009 | 0.0008 | 0.0023 | 0.0022 | 0.017 | 0.004 | 0.0007 | 0.0009 | 0.0011 |
Cytoplasmic Foci | 0.0262 | 0.0222 | 0.0191 | 0.0593 | 0.0797 | 0.0173 | 0.0253 | 0.0208 | 0.014 | 0.039 | 0.0713 | 0.0099 | 0.0234 | 0.021 | 0.0112 | 0.0226 | 0.1117 | 0.0071 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0002 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0012 | 0.0002 | 0.0003 | 0.0004 | 0.0005 | 0.0003 | 0.0007 | 0.0003 | 0.0005 | 0.0002 | 0 | 0.0002 |
Endosome | 0.0024 | 0.0018 | 0.0039 | 0.0042 | 0.0044 | 0.0031 | 0.0159 | 0.0021 | 0.0084 | 0.0253 | 0.0129 | 0.0024 | 0.0109 | 0.0042 | 0.0028 | 0.0103 | 0.0064 | 0.0032 |
Golgi | 0.001 | 0.0004 | 0.0007 | 0.0016 | 0.0021 | 0.0008 | 0.0029 | 0.0005 | 0.0012 | 0.0028 | 0.0057 | 0.0005 | 0.0036 | 0.0008 | 0.0025 | 0.0088 | 0.0039 | 0.0009 |
Lipid Particles | 0.008 | 0.0039 | 0.0049 | 0.0193 | 0.0497 | 0.0078 | 0.0243 | 0.0052 | 0.0084 | 0.0585 | 0.0881 | 0.0033 | 0.0196 | 0.0093 | 0.0042 | 0.0169 | 0.1003 | 0.0048 |
Mitochondria | 0.0001 | 0 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0046 | 0.0001 | 0.0007 | 0.0004 | 0.0013 | 0.0003 | 0.0075 | 0.0003 | 0.0126 | 0.005 | 0.0002 | 0.0016 |
None | 0.0086 | 0.0017 | 0.0114 | 0.0102 | 0.0003 | 0.0116 | 0.0151 | 0.0385 | 0.0105 | 0.0007 | 0.002 | 0.0161 | 0.0131 | 0.0541 | 0.0108 | 0.0028 | 0.0081 | 0.0093 |
Nuclear Periphery | 0.0034 | 0.0011 | 0.0008 | 0.0022 | 0.0006 | 0.0036 | 0.0087 | 0.0017 | 0.0041 | 0.0045 | 0.0132 | 0.0061 | 0.007 | 0.0076 | 0.0081 | 0.0032 | 0.0016 | 0.0053 |
Nucleolus | 0.0122 | 0.0278 | 0.0278 | 0.006 | 0.005 | 0.0184 | 0.0278 | 0.022 | 0.0338 | 0.0522 | 0.008 | 0.0323 | 0.0356 | 0.0558 | 0.0253 | 0.0288 | 0.0304 | 0.0369 |
Nucleus | 0.0195 | 0.0485 | 0.0134 | 0.0166 | 0.001 | 0.0137 | 0.0351 | 0.0765 | 0.0291 | 0.0328 | 0.0027 | 0.0399 | 0.0397 | 0.1523 | 0.0687 | 0.0461 | 0.0045 | 0.043 |
Peroxisomes | 0.0078 | 0.0009 | 0.002 | 0.0128 | 0.0148 | 0.002 | 0.0113 | 0.0057 | 0.0031 | 0.0059 | 0.0294 | 0.0023 | 0.0057 | 0.008 | 0.0119 | 0.0092 | 0.0225 | 0.0011 |
Punctate Nuclear | 0.9065 | 0.8878 | 0.9109 | 0.8656 | 0.8416 | 0.9172 | 0.8054 | 0.8075 | 0.8707 | 0.7738 | 0.7557 | 0.879 | 0.8063 | 0.6655 | 0.8178 | 0.8378 | 0.7076 | 0.8779 |
Vacuole | 0.0001 | 0.0002 | 0.0004 | 0 | 0.0001 | 0.0002 | 0.0018 | 0.0004 | 0.0043 | 0.0007 | 0.0006 | 0.0019 | 0.0039 | 0.0008 | 0.0007 | 0.0004 | 0.0002 | 0.0008 |
Vacuole Periphery | 0 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0.0012 | 0.0001 | 0.0007 | 0.0003 | 0.0006 | 0.0006 | 0.0016 | 0.0002 | 0.0007 | 0.0002 | 0.0001 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.9584 | 7.9976 | 13.8474 | 13.2175 | 9.0219 | 11.9221 | 13.4774 | 12.1284 | 16.6187 | 15.9076 |
Translational Efficiency | 1.8387 | 1.1967 | 1.0674 | 1.1194 | 1.0998 | 1.2929 | 1.2183 | 1.4308 | 1.214 | 1.0514 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
19 | 1241 | 417 | 127 | 1700 | 1484 | 203 | 1499 | 1719 | 2725 | 620 | 1626 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1402.70 | 771.96 | 765.64 | 861.74 | 723.25 | 792.35 | 940.20 | 820.00 | 730.76 | 783.06 | 822.79 | 823.26 |
Standard Deviation | 677.92 | 120.72 | 107.57 | 125.47 | 93.28 | 95.51 | 97.40 | 105.51 | 136.86 | 108.20 | 132.66 | 107.79 |
Intensity Change Log 2 | — | -0.861608 | -0.873468 | -0.702882 | — | 0.131643 | 0.378473 | 0.181129 | — | -0.442581 | -0.317625 | -0.338153 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.011479 | 0.000828 | 0.000862 | 0.000625 | 0.000544 | 0.000682 | 0.001780 | 0.000633 | 0.000665 | 0.000749 | 0.001163 | 0.000632 |
Bud Neck | 0.007609 | 0.010531 | 0.004308 | 0.003234 | 0.007498 | 0.006426 | 0.003149 | 0.006693 | 0.007499 | 0.008295 | 0.003928 | 0.006423 |
Bud Site | 0.016001 | 0.005597 | 0.004010 | 0.038225 | 0.003224 | 0.003374 | 0.005462 | 0.015616 | 0.003365 | 0.004386 | 0.004486 | 0.017382 |
Cell Periphery | 0.000658 | 0.000184 | 0.000158 | 0.000084 | 0.000168 | 0.000158 | 0.000179 | 0.000134 | 0.000173 | 0.000169 | 0.000165 | 0.000130 |
Cytoplasm | 0.143199* | 0.053762 | 0.070931 | 0.050052 | 0.062841 | 0.072488 | 0.027314 | 0.079707 | 0.063730 | 0.063960 | 0.056650 | 0.077391 |
Cytoplasmic Foci | 0.021419 | 0.084527 | 0.012003 | 0.015163 | 0.060496 | 0.085253 | 0.009855 | 0.025159 | 0.060064 | 0.084922 | 0.011299 | 0.024379 |
Eisosomes | 0.000198 | 0.000088 | 0.000098 | 0.000045 | 0.000101 | 0.000088 | 0.000160 | 0.000048 | 0.000102 | 0.000088 | 0.000118 | 0.000048 |
Endoplasmic Reticulum | 0.020530 | 0.002921 | 0.004019 | 0.001916 | 0.002065 | 0.001335 | 0.009655 | 0.000980 | 0.002269 | 0.002058 | 0.005864 | 0.001053 |
Endosome | 0.016500 | 0.012025 | 0.003973 | 0.012419 | 0.009482 | 0.020939 | 0.004754 | 0.009797 | 0.009560 | 0.016880 | 0.004229 | 0.010002 |
Golgi | 0.004341 | 0.008078 | 0.001679 | 0.003250 | 0.006484 | 0.011183 | 0.001090 | 0.003261 | 0.006460 | 0.009769 | 0.001486 | 0.003260 |
Lipid Particles | 0.014207 | 0.014681 | 0.002679 | 0.003163 | 0.014346 | 0.026471 | 0.004998 | 0.004292 | 0.014345 | 0.021101 | 0.003438 | 0.004204 |
Mitochondria | 0.001554 | 0.008268 | 0.000907 | 0.001023 | 0.003695 | 0.010664 | 0.000878 | 0.004900 | 0.003672 | 0.009573 | 0.000898 | 0.004597 |
Mitotic Spindle | 0.002072 | 0.426892* | 0.407697* | 0.709330* | 0.235823* | 0.354378* | 0.464579* | 0.651287* | 0.233239* | 0.387402* | 0.426321* | 0.655821* |
None | 0.003390 | 0.019803 | 0.016778 | 0.007585 | 0.019852 | 0.015392 | 0.005238 | 0.011318 | 0.019670 | 0.017401 | 0.013000 | 0.011026 |
Nuclear Periphery | 0.004272 | 0.001799 | 0.001107 | 0.000734 | 0.001930 | 0.002664 | 0.001038 | 0.000758 | 0.001956 | 0.002270 | 0.001084 | 0.000756 |
Nuclear Periphery Foci | 0.121106 | 0.092136 | 0.080562 | 0.044509 | 0.094075 | 0.054381 | 0.055023 | 0.012867 | 0.094374 | 0.071575 | 0.072200 | 0.015339 |
Nucleolus | 0.082369 | 0.109011 | 0.103819 | 0.016066 | 0.265390* | 0.162712 | 0.038615 | 0.043709 | 0.263367* | 0.138256 | 0.082470 | 0.041550 |
Nucleus | 0.358496* | 0.123205 | 0.270763* | 0.069958 | 0.191621* | 0.142493* | 0.332588* | 0.103155 | 0.193466* | 0.133709* | 0.291006* | 0.100562 |
Peroxisomes | 0.013439 | 0.021546 | 0.007249 | 0.001562 | 0.016692 | 0.024690 | 0.009431 | 0.007153 | 0.016656 | 0.023258 | 0.007963 | 0.006716 |
Vacuole | 0.147103 | 0.003390 | 0.006166 | 0.019857 | 0.003349 | 0.003525 | 0.023895 | 0.015180 | 0.004938 | 0.003463 | 0.011971 | 0.015545 |
Vacuole Periphery | 0.010058 | 0.000728 | 0.000231 | 0.001199 | 0.000323 | 0.000705 | 0.000319 | 0.003352 | 0.000431 | 0.000716 | 0.000260 | 0.003184 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.74 | 1.59 | 1.60 | -2.71 | 0.27 | -0.46 | -7.25 | -5.01 | -4.55 | 6.06 | -0.84 | -8.93 | -2.98 | -3.14 | 7.54 |
Bud Neck | 0.47 | 0.12 | 0.48 | 0.09 | 0.63 | 0.30 | -3.55 | -6.56 | -6.86 | -0.75 | -2.66 | -5.97 | -6.65 | -4.55 | -0.77 |
Bud Site | 1.55 | 0.67 | 0.07 | -1.77 | -0.70 | -1.51 | -6.98 | -7.92 | -7.46 | -0.02 | -4.37 | -10.72 | -8.10 | -6.83 | -0.73 |
Cell Periphery | 2.38 | 2.30 | 2.45 | 0.79 | 1.48 | 1.06 | -5.29 | 2.25 | 1.24 | 6.32 | -0.19 | -4.93 | 2.63 | 3.08 | 7.16 |
Cytoplasm | 2.42 | 2.50 | 2.33 | -0.17 | -0.54 | -1.59 | 0.78 | -6.10 | -4.62 | -4.73 | -1.42 | 0.51 | -5.56 | -4.81 | -5.14 |
Cytoplasmic Foci | -5.29 | 0.78 | 1.42 | 10.00 | 1.30 | -3.39 | 9.85 | 8.53 | 11.04 | -2.79 | -3.68 | 11.84 | 9.02 | 13.92 | -3.13 |
Eisosomes | 1.59 | 0.79 | 1.69 | 0.55 | 2.82 | 5.38 | -5.49 | 9.01 | 4.25 | 7.60 | 5.81 | -5.02 | 9.04 | 4.68 | 9.36 |
Endoplasmic Reticulum | 3.63 | 3.10 | 2.77 | -3.39 | -1.12 | 5.56 | -6.68 | -2.87 | -6.84 | 6.26 | 0.78 | -9.63 | -3.09 | -3.81 | 8.20 |
Endosome | 1.37 | 2.05 | 1.84 | 1.42 | -0.24 | -5.15 | -3.80 | -1.95 | 2.97 | 2.09 | -5.75 | -2.17 | -1.82 | 3.04 | 0.02 |
Golgi | -3.89 | 0.15 | 1.55 | 9.34 | 3.08 | 1.25 | 7.87 | 11.77 | 9.07 | 3.36 | -0.61 | 9.33 | 12.10 | 13.41 | 4.82 |
Lipid Particles | 0.19 | 2.23 | 2.23 | 8.52 | 0.03 | -3.37 | 2.45 | 6.73 | 8.04 | 3.13 | -3.93 | 6.89 | 7.07 | 10.82 | 0.81 |
Mitochondria | -2.03 | 1.44 | 1.31 | 2.99 | -0.18 | -1.64 | 4.88 | 0.21 | 1.47 | -1.43 | -2.57 | 5.48 | 0.34 | 2.23 | -1.45 |
Mitotic Spindle | -40.39* | -23.51* | -18.25 | -5.04 | -4.56 | -10.90 | -5.83 | -25.66* | -13.90 | -5.81 | -14.42 | -10.00 | -26.75 | -14.99 | -8.99 |
None | -2.16 | -1.80 | 0.19 | 5.05 | 3.60 | 3.09 | 8.93 | 7.12 | 4.59 | -2.47 | 2.14 | 3.02 | 7.44 | 6.13 | 2.18 |
Nuclear Periphery | 1.34 | 1.33 | 0.74 | -1.66 | -1.65 | -0.04 | -2.16 | -1.87 | -1.14 | 0.96 | 0.58 | -0.30 | -2.02 | -2.43 | -1.85 |
Nuclear Periphery Foci | 0.38 | 0.34 | 0.95 | 1.86 | 1.75 | 7.63 | 0.17 | 17.84 | 9.97 | 7.43 | 6.18 | 1.58 | 17.14 | 13.52 | 10.11 |
Nucleolus | -2.56 | -2.68 | 0.78 | 8.51 | 7.58 | 9.43 | 16.07 | 25.29* | 14.69 | 0.89 | 14.99* | 17.27 | 26.11 | 13.94 | 4.76 |
Nucleus | 3.89 | 2.77 | 3.21 | -1.81 | 1.53 | 4.18 | -3.84 | -1.06 | -4.81 | 3.46 | 4.08 | -5.75 | -0.86 | -4.61 | 5.12 |
Peroxisomes | -1.75 | 0.24 | 1.64 | 9.87 | 4.04 | -2.90 | -0.75 | 4.45 | 6.75 | 2.82 | -4.34 | 0.72 | 4.85 | 9.97 | 3.19 |
Vacuole | 4.26 | 4.16 | 3.71 | -3.66 | -2.89 | 0.20 | -4.28 | -7.95 | -8.05 | 1.50 | 1.75 | -4.40 | -7.21 | -8.34 | -1.53 |
Vacuole Periphery | 1.65 | 1.67 | 1.64 | -0.43 | -1.02 | -1.73 | -6.37 | -1.84 | -1.61 | -1.02 | -0.45 | -0.97 | -1.54 | -1.46 | -1.36 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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