Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the small subunit
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0.05
Bud 0 0 0.09 0.05 0.06 0 – 0 0 0.05 0 0 0 0 0 0.08 0.05 0.08 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.08 0.06 0.15 0.06 0.05 0 – 0 0 0 0 0 0.07 0.13 0.13 0.29 0.28 0.32 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0 0.14 0.07 0.07 0 0 0 0 0 0
Endosome 0 0.06 0 0 0.09 0 – 0 0 0 0 0 0.2 0.22 0.22 0.09 0.12 0.14 0.09 0.14 0.1 0.11 0.13 0.11
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0.05 0.06 0.08
Mitochondria 0.84 0.82 0.83 0.92 0.82 0.93 – 0.92 0.94 0.96 0.96 0.96 0.61 0.52 0.47 0.15 0.21 0.17 0.68 0.62 0.71 0.63 0.56 0.51
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0.06 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0.06 0.05 0 0 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.05 0 – 0 0 0 0 0 0.08 0.08 0.11 0 0.1 0.05 0 0 0.06 0.06 0.09 0.06
SpindlePole 0 0 0.07 0 0.07 0 – 0 0 0.05 0 0 0 0 0 0.18 0.19 0.19 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.11 0 0 0 0 – 0 0 0 0 0 0.15 0.12 0.12 0.12 0.09 0.08 0.1 0.06 0 0 0 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 3 4 0 0 0 – 1 0 0 0 0 2 1 0 1 2 6 7 2 8 2 10 15
Bud 5 0 32 17 23 20 – 21 30 31 17 26 2 8 2 5 3 7 2 0 0 0 2 3
Bud Neck 1 0 8 0 0 0 – 0 0 0 0 0 1 0 0 1 4 1 3 1 3 1 5 7
Bud Site 2 0 3 4 1 5 – 9 18 18 7 19 0 0 0 1 1 1 – – – – – –
Cell Periphery 2 2 2 7 2 2 – 4 2 3 5 1 6 5 7 2 2 3 1 1 0 0 0 0
Cytoplasm 34 6 56 21 19 16 – 20 15 10 8 10 15 30 30 19 16 28 5 0 3 2 3 3
Endoplasmic Reticulum 12 5 10 4 3 3 – 0 1 3 0 4 4 8 5 9 4 6 1 0 3 3 2 2
Endosome 16 6 0 0 37 4 – 12 2 2 1 1 45 51 50 6 7 12 41 16 40 24 43 36
Golgi 0 0 0 1 0 0 – 0 0 0 0 0 15 5 7 0 0 0 8 3 2 12 18 26
Mitochondria 362 81 300 308 337 502 – 767 673 577 475 592 139 119 107 10 12 15 317 72 284 141 182 165
Nucleus 8 1 1 0 5 9 – 18 11 6 3 8 1 1 1 2 1 1 2 0 1 0 0 4
Nuclear Periphery 1 2 0 2 6 15 – 28 43 18 27 36 1 0 0 0 1 0 1 0 1 1 3 1
Nucleolus 0 0 1 4 5 15 – 51 37 25 20 33 1 2 0 3 1 0 2 2 0 1 4 3
Peroxisomes 3 2 2 13 22 12 – 19 18 16 6 11 18 18 25 0 6 4 15 4 25 13 28 18
SpindlePole 12 1 27 6 29 18 – 34 18 28 10 23 7 7 8 12 11 17 6 2 6 4 3 3
Vac/Vac Membrane 70 11 9 2 16 23 – 22 20 9 9 8 35 27 27 8 5 7 45 7 7 10 13 24
Unique Cell Count 432 99 363 333 410 538 830 714 604 497 618 229 231 227 65 58 88 471 118 399 225 327 325
Labelled Cell Count 530 120 455 389 505 644 1006 888 746 588 772 292 282 269 79 76 108 471 118 399 225 327 325


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 4.7 3.5 3.9 4.6 4.1 – 4.1 3.8 4.0 4.0 3.9 5.6 5.6 5.5 5.3 5.6 5.1 6.0 6.3 6.0
Std Deviation (1e-4) 0.6 0.8 0.8 1.7 2.1 1.4 – 1.1 1.2 1.0 1.0 1.0 1.5 1.5 1.5 1.2 1.7 1.3 2.2 2.2 1.6
Intensity Change (Log2) – – – 0.17 0.41 0.25 – 0.25 0.15 0.22 0.21 0.18 0.7 0.68 0.67 0.62 0.68 0.55 0.78 0.85 0.8


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8936 3.2542 3.4113 2.7063 2.9633 3.3487 3.0024 2.737 3.2304 2.7029 2.9456 2.7732 5.3041 4.7888 4.5038 4.7849 3.7735 4.2159
Actin 0.0287 0.0078 0.0043 0.0138 0.0041 0.0034 0.018 0.0045 0.0142 0.0011 0.0277 0.0045 0.0938 0.0176 0.0174 0.0537 0.0426 0.0179
Bud 0.0006 0.0029 0.0022 0.0004 0.0001 0.0002 0.0004 0.0007 0.0004 0.0009 0.0006 0.0005 0.0017 0.0009 0.0005 0.0001 0.0002 0.0002
Bud Neck 0.0019 0.0004 0.0002 0.0002 0.0005 0.0002 0.0004 0.0002 0.0006 0.0002 0.0005 0.0004 0.0138 0.0003 0.0004 0.0002 0.0003 0.0013
Bud Periphery 0.0011 0.0065 0.0039 0.001 0.0003 0.0006 0.0007 0.0018 0.0012 0.0031 0.0009 0.0024 0.0028 0.003 0.0007 0.0002 0.0004 0.0006
Bud Site 0.0043 0.0039 0.002 0.0007 0.0004 0.0003 0.0008 0.0018 0.0011 0.0003 0.0018 0.0003 0.0219 0.0113 0.0022 0.001 0.0004 0.0004
Cell Periphery 0.0006 0.0005 0.0002 0.0004 0.0001 0.0002 0.0002 0.0002 0.0027 0.0003 0.0002 0.0002 0.002 0.0018 0.0002 0.0001 0.0001 0.0002
Cytoplasm 0.0044 0.0016 0.0003 0.0005 0.0046 0.0015 0.0013 0.0008 0.0085 0.012 0.002 0.0002 0.0151 0.0007 0.0014 0.0001 0.0001 0.0002
Cytoplasmic Foci 0.0178 0.0177 0.0072 0.011 0.0096 0.0061 0.0138 0.0115 0.0079 0.013 0.01 0.0038 0.0445 0.0061 0.0077 0.0032 0.0041 0.0055
Eisosomes 0.0004 0.0002 0.0004 0.0018 0.0002 0.0002 0.0003 0.0002 0.0005 0.0002 0.0003 0.0002 0.0013 0.0004 0.0002 0.0002 0.0002 0.0004
Endoplasmic Reticulum 0.0065 0.0003 0.0003 0.0002 0.002 0.0006 0.0023 0.0005 0.0026 0.0002 0.0039 0.0002 0.0081 0.0005 0.0004 0 0.0001 0.0001
Endosome 0.0632 0.0496 0.0428 0.0169 0.073 0.0253 0.045 0.0505 0.0333 0.0491 0.0844 0.0158 0.0685 0.0348 0.0181 0.011 0.0108 0.0196
Golgi 0.0545 0.081 0.0736 0.0249 0.086 0.0436 0.0494 0.0625 0.0405 0.0338 0.0565 0.0325 0.0728 0.0561 0.0444 0.0427 0.0298 0.0353
Lipid Particles 0.0317 0.0387 0.006 0.0075 0.0883 0.0076 0.0316 0.0134 0.0145 0.0027 0.0085 0.0066 0.0428 0.0096 0.0095 0.0018 0.0041 0.006
Mitochondria 0.6999 0.754 0.8395 0.8673 0.6518 0.8816 0.7892 0.8253 0.844 0.8443 0.7722 0.9194 0.4846 0.826 0.8853 0.8728 0.8882 0.8995
None 0.0045 0.0008 0.0001 0.0008 0.0112 0.0002 0.003 0.0005 0.0031 0.0023 0.0008 0.0001 0.011 0.0002 0.0002 0 0 0.0001
Nuclear Periphery 0.0101 0.0002 0.0001 0.0014 0.0137 0.0062 0.0076 0.0001 0.0045 0.0002 0.0043 0.0004 0.0086 0.0003 0.0004 0 0 0.0001
Nucleolus 0.0007 0.0004 0.0001 0.0002 0.0014 0.0002 0.0006 0.0001 0.0012 0.0002 0.0004 0.0001 0.0084 0.0003 0.0001 0 0 0.0001
Nucleus 0.0056 0.0002 0.0001 0.0002 0.007 0.0061 0.0015 0.0001 0.0017 0.0003 0.0014 0.0001 0.025 0.0002 0.0002 0 0 0.0001
Peroxisomes 0.0423 0.0232 0.0114 0.0378 0.0188 0.0095 0.0213 0.0161 0.0089 0.0162 0.013 0.0088 0.0395 0.016 0.0069 0.0112 0.0155 0.0097
Punctate Nuclear 0.0036 0.0001 0 0.0089 0.0182 0.003 0.005 0.0001 0.0013 0.0001 0.0022 0.0003 0.0178 0.0001 0.001 0 0 0.0003
Vacuole 0.01 0.0053 0.002 0.0011 0.0022 0.0013 0.0031 0.0048 0.0038 0.0123 0.0034 0.0016 0.0123 0.0094 0.0012 0.0004 0.0007 0.0009
Vacuole Periphery 0.0077 0.0048 0.0033 0.0031 0.0063 0.0021 0.0043 0.0044 0.0034 0.0075 0.0049 0.0016 0.0038 0.0042 0.0019 0.001 0.0022 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.0738 26.9502 11.9989 33.8563 25.407 30.131 35.4241 43.2199 39.3607 45.6798
Translational Efficiency 4.5249 1.9596 4.2284 2.6075 2.4388 3.1958 1.7298 1.7939 1.8928 1.6792

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1832 95 206 945 827 1373 819 1702 2659 1468 1025 2647

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 672.15 794.30 902.73 907.90 580.36 752.49 879.68 896.66 643.60 755.20 884.31 900.67
Standard Deviation 77.59 111.90 114.88 120.58 114.53 100.37 105.81 115.36 100.17 101.68 108.09 117.38
Intensity Change Log 2 — 0.240901 0.425511 0.433750 — 0.374724 0.600031 0.627613 — 0.304455 0.509007 0.526825

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000614 0.007583 0.013644 0.016843 0.000687 0.001590 0.001811 0.022644 0.000637 0.001978 0.004189 0.020573
Bud Neck 0.010113 0.011804 0.012122 0.003670 0.035573 0.007589 0.014474 0.004085 0.018032 0.007862 0.014001 0.003937
Bud Site 0.005709 0.034601 0.041822 0.039717 0.011512 0.010479 0.024295 0.057641 0.007514 0.012040 0.027817 0.051242
Cell Periphery 0.000360 0.002879 0.000426 0.000705 0.001093 0.000275 0.000197 0.001091 0.000588 0.000444 0.000243 0.000953
Cytoplasm 0.009299 0.009197 0.006437 0.007754 0.034017 0.006300 0.006640 0.016036 0.016986 0.006488 0.006599 0.013079
Cytoplasmic Foci 0.067550 0.085245 0.103555 0.073372 0.083031 0.075948 0.051319 0.051198 0.072365 0.076550 0.061817 0.059115
Eisosomes 0.000134 0.000236 0.000150 0.000145 0.000360 0.000161 0.000089 0.000206 0.000205 0.000166 0.000101 0.000184
Endoplasmic Reticulum 0.000803 0.001351 0.003214 0.001197 0.001176 0.000344 0.001306 0.000714 0.000919 0.000409 0.001689 0.000887
Endosome 0.097832 0.119563 0.140622 0.107079 0.081718 0.103458 0.146316 0.061772 0.092820 0.104500 0.145171 0.077947
Golgi 0.126602* 0.147454* 0.098250 0.133246* 0.080265 0.161188* 0.064077 0.135637* 0.112190* 0.160299* 0.070945 0.134784*
Lipid Particles 0.010870 0.016075 0.008721 0.001728 0.033119 0.004157 0.009963 0.000425 0.017789 0.004928 0.009714 0.000890
Mitochondria 0.596721* 0.385906* 0.283926* 0.448367* 0.509648* 0.524385* 0.422437* 0.509773* 0.569640* 0.515424* 0.394600* 0.487851*
Mitotic Spindle 0.004327 0.009166 0.067800 0.052323 0.005945 0.008956 0.097065 0.032905 0.004830 0.008970 0.091184 0.039837
None 0.000614 0.000164 0.000358 0.000289 0.011393 0.000305 0.000564 0.000180 0.003966 0.000296 0.000522 0.000219
Nuclear Periphery 0.000297 0.000125 0.000468 0.000129 0.000588 0.000142 0.001798 0.000040 0.000388 0.000141 0.001531 0.000072
Nuclear Periphery Foci 0.000302 0.000885 0.002560 0.000330 0.001118 0.000166 0.002200 0.000076 0.000556 0.000213 0.002272 0.000167
Nucleolus 0.000660 0.000402 0.004496 0.000200 0.007604 0.000972 0.003895 0.000063 0.002820 0.000936 0.004015 0.000112
Nucleus 0.001333 0.000954 0.009734 0.000305 0.017321 0.001595 0.006342 0.000358 0.006305 0.001553 0.007024 0.000339
Peroxisomes 0.039176 0.136400* 0.138863* 0.074117 0.066284 0.067087 0.045777 0.083999 0.047607 0.071572 0.064485 0.080471
Vacuole 0.012764 0.020999 0.042535 0.024433 0.007864 0.012927 0.068529 0.014575 0.011240 0.013450 0.063305 0.018094
Vacuole Periphery 0.013920 0.009010 0.020296 0.014049 0.009686 0.011977 0.030909 0.006582 0.012603 0.011785 0.028776 0.009248

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.27 -2.67 -7.31 -2.44 -0.62 -2.78 -1.80 -11.39 -10.80 -10.30 -3.69 -3.20 -13.54 -12.31 -8.93
Bud Neck -0.45 -0.92 5.12 2.23 4.10 6.84 4.87 7.83 3.43 6.51 5.92 1.94 9.24 4.13 7.55
Bud Site -3.62 -4.70 -10.40 -0.65 0.18 0.45 -4.65 -13.68 -14.63 -8.83 -3.15 -8.22 -18.82 -15.22 -7.15
Cell Periphery -1.25 -0.57 -2.10 1.08 -1.75 4.82 5.36 -0.00 -3.87 -4.27 0.93 4.35 -2.24 -2.59 -4.91
Cytoplasm 0.08 1.61 0.75 0.40 -1.04 6.91 6.73 4.44 -7.55 -6.86 6.17 6.03 2.26 -6.21 -6.06
Cytoplasmic Foci -1.86 -3.95 -1.44 1.20 3.22 1.74 5.96 6.45 7.34 0.13 -0.93 3.02 4.97 5.52 0.87
Eisosomes -2.32 -1.01 -0.59 2.18 0.67 3.46 5.08 2.85 -1.77 -9.50 1.33 4.99 0.97 -0.80 -8.83
Endoplasmic Reticulum -1.10 -2.71 -1.13 0.32 2.24 2.39 -0.49 1.16 -2.18 2.90 2.32 -2.86 0.01 -2.94 3.26
Endosome -1.19 -3.36 -1.58 0.69 2.58 -3.66 -8.13 3.74 8.90 12.16 -2.44 -8.14 4.07 5.88 10.88
Golgi -1.19 2.67 -0.84 0.83 -3.02 -11.44 2.64 -8.45 3.71 -11.92 -8.01 8.33 -4.62 4.20 -12.30
Lipid Particles -0.89 0.76 7.66 2.40 3.79 7.89 5.63 9.02 6.03 4.99 8.27 3.75 12.10 5.69 5.63
Mitochondria 6.04 12.94 10.45 -1.67 -6.37 -1.16 4.86 -0.23 1.17 -6.01 4.65 13.72 8.23 2.47 -7.34
Mitotic Spindle -1.74 -5.83 -11.52 -8.70 1.27 -2.07 -13.05 -10.49 -10.17 8.83 -4.43 -14.51 -16.27 -14.16 8.12
None 1.81 0.89 1.22 -1.28 0.78 4.76 4.64 4.81 2.68 2.28 4.88 4.52 4.99 1.43 2.21
Nuclear Periphery 1.14 -1.11 1.18 -0.10 4.26 3.39 -2.05 4.75 1.46 3.05 2.07 -2.42 3.05 1.05 3.17
Nuclear Periphery Foci -1.23 -2.86 -0.76 1.07 2.78 3.58 -1.45 3.94 2.36 2.84 3.24 -2.90 3.76 0.55 3.48
Nucleolus 0.98 -1.79 2.60 0.82 1.93 4.33 2.26 4.96 3.84 5.05 3.54 -1.50 5.49 3.70 4.84
Nucleus 0.73 -2.28 3.29 1.51 2.53 6.34 3.85 7.00 2.24 5.40 5.02 -0.93 7.45 2.38 5.76
Peroxisomes -4.21 -6.43 -6.21 2.64 4.01 -0.09 3.33 -2.67 -2.76 -6.43 -5.07 -3.40 -8.16 -1.62 -2.75
Vacuole -0.99 -5.19 -5.18 -0.47 2.86 -3.27 -13.94 -4.31 -1.25 12.15 -1.58 -14.28 -5.48 -3.18 11.94
Vacuole Periphery 2.74 -2.13 -0.06 -2.55 2.03 -1.69 -8.89 2.67 5.05 11.01 0.71 -8.32 3.96 2.46 10.32
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the small subunit
Localization
Cell Percentages mitochondrion (90%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Mrps16

Mrps16


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrps16-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available