Standard name
Human Ortholog
Description Mitochondrial transporter; major substrates are adenosine 5'-phosphosulfate (APS) and 3'-phospho-adenosine 5'-phosphosulfate (PAPS); member of the mitochondrial carrier family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0.06 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0.05 0.05 0 0 0 0.06 0.06 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 0.59 0.61 0.63 0.49 0.48 0.42 0.51 – 0.46 0.36 0.33 0.86 0.91 0.92 0.59 0.44 0.65 0.1 0.18 0.2 0.17 0.14 0.2
Endoplasmic Reticulum 0.12 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.1 0.06 0 0 0 0 0 0
Endosome 0 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.12 0.08 0.11 0.05 0.05 0.07 0 0.05
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.62 0.24 0.47 0.41 0.56 0.62 0.67 0.56 – 0.6 0.74 0.76 0 0 0 0.14 0.15 0.06 0.61 0.55 0.54 0.52 0.56 0.51
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.06 0 0 0 0.05 0 0.05 0.07 – 0.08 0.07 0.05 0 0 0 0.11 0.12 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0 0.1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.12 0.06 0 0.08 0 0.1 0.1 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 0 1 0 0 0 0 0 – 0 0 0 7 2 1 6 1 3 5 4 6 0 3 4
Bud 1 2 3 8 16 24 28 22 – 19 27 44 6 4 6 6 4 3 0 1 0 1 2 1
Bud Neck 1 0 0 1 1 3 6 1 – 4 1 2 4 4 9 4 3 1 1 2 1 2 2 5
Bud Site 1 0 1 0 0 14 6 4 – 9 13 9 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 2 0 4 1 0 2 4 – 0 1 5 3 1 3 1 1 2 0 0 0 0 1 0
Cytoplasm 42 80 81 159 208 291 354 424 – 363 206 280 118 333 424 59 30 57 18 33 30 15 24 40
Endoplasmic Reticulum 17 4 2 1 0 5 1 1 – 1 1 2 2 6 2 2 7 5 0 3 2 0 4 2
Endosome 1 8 1 7 12 2 3 4 – 6 1 1 0 8 6 3 8 7 21 9 8 6 3 9
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 2 0 1 1 1 0 0 1
Mitochondria 87 32 63 103 236 378 563 463 – 474 423 636 6 5 8 14 10 5 116 99 85 46 100 103
Nucleus 0 1 0 0 3 4 11 21 – 6 5 7 1 2 0 2 1 5 0 0 0 0 1 0
Nuclear Periphery 2 0 0 0 1 2 3 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 1 0 0 0 2 0 3 – 1 4 3 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 1 7 17 3 6 2 – 3 3 4 0 1 11 1 1 0 4 0 4 0 0 0
SpindlePole 8 2 3 11 21 21 44 55 – 64 38 39 2 3 7 11 8 7 5 5 5 1 8 3
Vac/Vac Membrane 4 13 1 4 14 15 9 10 – 21 11 5 3 6 12 3 8 5 5 14 4 8 18 23
Unique Cell Count 140 135 133 254 422 605 842 834 793 572 842 138 367 459 100 68 88 192 180 158 89 179 201
Labelled Cell Count 169 145 157 305 530 764 1036 1014 972 734 1037 152 375 489 113 84 100 192 180 158 89 179 201


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.4 3.4 3.4 3.4 3.3 2.6 3.0 – 2.7 2.6 2.6 4.8 4.8 4.7 4.9 4.9 7.0 5.4 5.6 5.3
Std Deviation (1e-4) 0.5 1.0 0.9 1.4 1.4 1.5 1.1 1.4 – 1.2 1.0 0.8 1.0 1.0 1.0 1.1 1.3 19.0 1.5 1.9 1.6
Intensity Change (Log2) – – – -0.01 -0.01 -0.05 -0.38 -0.18 – -0.33 -0.37 -0.41 0.49 0.51 0.47 0.54 0.54 1.03 0.67 0.72 0.64


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4865 0.4721 0.5589 0.2583 0.247 0.3874 0.9136 1.098 0.9129 0.9856 0.6091 1.0879 -1.4283 -0.9168 -1.3489 -0.6622 -1.4505 -1.0851
Actin 0.014 0.0092 0.0094 0.0098 0.0018 0.008 0.0153 0.0206 0.0096 0.008 0.0027 0.0098 0.005 0.0235 0.0095 0.0139 0.0318 0.0128
Bud 0.0009 0.0037 0.0042 0.0077 0.0014 0.0018 0.0009 0.0035 0.0025 0.0019 0.0013 0.0009 0.0006 0.0013 0.0037 0.0034 0.0016 0.0011
Bud Neck 0.0109 0.0022 0.0096 0.0009 0.0006 0.0041 0.0068 0.0021 0.0036 0.0198 0.0008 0.0246 0.0073 0.0008 0.0076 0.0141 0.0016 0.0184
Bud Periphery 0.0019 0.0054 0.0049 0.0378 0.0026 0.0043 0.002 0.0089 0.0058 0.0146 0.0036 0.0033 0.0014 0.0024 0.0089 0.0069 0.0044 0.0035
Bud Site 0.0144 0.0393 0.0156 0.0038 0.0013 0.0033 0.0185 0.0305 0.0119 0.0132 0.0027 0.0012 0.0123 0.0265 0.0149 0.0117 0.0017 0.0015
Cell Periphery 0.0012 0.0014 0.0008 0.0005 0.0005 0.0004 0.0021 0.0014 0.0028 0.0009 0.0009 0.0011 0.0016 0.0047 0.0012 0.0013 0.0003 0.001
Cytoplasm 0.0153 0.0567 0.0299 0.0224 0.0367 0.0209 0.0221 0.0276 0.0299 0.0125 0.015 0.0226 0.0227 0.0364 0.0262 0.0384 0.0338 0.0372
Cytoplasmic Foci 0.105 0.1321 0.1274 0.1282 0.1151 0.1302 0.1383 0.1109 0.1128 0.1378 0.0813 0.0921 0.093 0.1213 0.1042 0.1105 0.1412 0.081
Eisosomes 0.002 0.0005 0.0005 0.0001 0.0002 0.0004 0.0011 0.0014 0.0012 0.0006 0.0005 0.0005 0.0015 0.0016 0.0008 0.001 0.0005 0.0004
Endoplasmic Reticulum 0.0022 0.0018 0.0022 0.0016 0.0029 0.0011 0.0022 0.0011 0.0019 0.001 0.0027 0.0011 0.0083 0.0017 0.0029 0.0036 0.002 0.0024
Endosome 0.1578 0.1315 0.1477 0.1638 0.1496 0.1144 0.1187 0.1066 0.1086 0.1208 0.1279 0.0987 0.1723 0.1384 0.1268 0.0564 0.1545 0.1368
Golgi 0.0628 0.0578 0.0528 0.0447 0.069 0.0571 0.0631 0.0647 0.0558 0.0437 0.067 0.0626 0.0813 0.0917 0.0589 0.0275 0.1221 0.0641
Lipid Particles 0.101 0.0267 0.0375 0.042 0.0135 0.0332 0.0705 0.0159 0.0267 0.019 0.0176 0.0362 0.082 0.0314 0.0409 0.0257 0.077 0.052
Mitochondria 0.2913 0.3153 0.3552 0.3793 0.4905 0.4133 0.3154 0.4241 0.413 0.4659 0.433 0.441 0.3274 0.359 0.4361 0.499 0.2708 0.4524
None 0.0819 0.0757 0.0684 0.0421 0.0249 0.0576 0.0972 0.0665 0.0967 0.0235 0.1153 0.0751 0.0755 0.069 0.0419 0.079 0.0093 0.0278
Nuclear Periphery 0.0032 0.001 0.0049 0.0008 0.0014 0.0017 0.001 0.0004 0.001 0.0004 0.0009 0.0003 0.0088 0.0008 0.0024 0.0009 0.0029 0.0061
Nucleolus 0.0039 0.002 0.0025 0.0007 0.0005 0.001 0.0019 0.0012 0.0011 0.001 0.0007 0.001 0.002 0.0007 0.0017 0.0034 0.0007 0.0016
Nucleus 0.003 0.0018 0.0022 0.0008 0.0009 0.0017 0.0012 0.0011 0.0013 0.0009 0.0009 0.0007 0.0031 0.0011 0.0015 0.0017 0.0006 0.0013
Peroxisomes 0.1008 0.1188 0.1052 0.0956 0.0715 0.131 0.1087 0.0999 0.0948 0.1001 0.1123 0.1154 0.0648 0.0685 0.0942 0.047 0.1316 0.0802
Punctate Nuclear 0.0071 0.0014 0.0028 0.0011 0.0021 0.005 0.0034 0.0011 0.001 0.0027 0.0009 0.0008 0.0042 0.0007 0.003 0.0395 0.0041 0.0012
Vacuole 0.0154 0.0112 0.0123 0.0097 0.0078 0.006 0.0068 0.0076 0.0132 0.0079 0.0079 0.0059 0.0186 0.0137 0.0091 0.0101 0.0044 0.0108
Vacuole Periphery 0.0038 0.0043 0.0041 0.0066 0.0052 0.0037 0.0028 0.0028 0.0046 0.0039 0.0043 0.0051 0.0062 0.005 0.0036 0.0048 0.003 0.0064

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.9349 14.0767 11.1916 14.4503 13.854 12.3657 14.233 13.7233 20.0576 17.4612
Translational Efficiency 0.621 0.5791 0.6787 0.6123 0.5019 0.6798 0.6662 0.8453 0.4909 0.4573

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1284 647 1782 120 1627 1706 470 1223 2911 2353 2252 1343

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 620.74 679.48 812.71 866.62 654.92 699.79 846.17 776.79 639.84 694.21 819.69 784.82
Standard Deviation 69.82 92.98 93.60 99.00 98.42 89.24 87.17 111.63 88.61 90.74 93.29 113.49
Intensity Change Log 2 — 0.130442 0.388752 0.481410 — 0.095603 0.369629 0.246206 — 0.112661 0.378966 0.365449

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000436 0.001980 0.000338 0.014887 0.000570 0.000898 0.002708 0.004651 0.000511 0.001196 0.000833 0.005565
Bud Neck 0.049529 0.067262 0.014526 0.008953 0.034935 0.037533 0.034332 0.026203 0.041372 0.045707 0.018660 0.024661
Bud Site 0.015239 0.046430 0.008411 0.059063 0.016841 0.019387 0.041492 0.045879 0.016134 0.026823 0.015315 0.047057
Cell Periphery 0.000290 0.000202 0.000092 0.000485 0.000251 0.000356 0.000178 0.000266 0.000268 0.000313 0.000110 0.000286
Cytoplasm 0.144729* 0.127334* 0.437870* 0.276029* 0.091919 0.056674 0.301704* 0.412260* 0.115212* 0.076103 0.409452* 0.400088*
Cytoplasmic Foci 0.317538* 0.268485* 0.069428 0.073901 0.289455* 0.210602* 0.125373 0.107232 0.301842* 0.226518* 0.081104 0.104254
Eisosomes 0.000313 0.000206 0.000042 0.000486 0.000274 0.000285 0.000110 0.000170 0.000291 0.000263 0.000056 0.000198
Endoplasmic Reticulum 0.003168 0.001438 0.003843 0.008667 0.002022 0.000598 0.005122 0.002402 0.002527 0.000829 0.004110 0.002962
Endosome 0.078767 0.079244 0.012538 0.010112 0.089113 0.094663 0.022024 0.011219 0.084550 0.090423 0.014518 0.011120
Golgi 0.032112 0.041232 0.001199 0.000745 0.065245 0.083413 0.001692 0.004336 0.050630 0.071815 0.001302 0.004015
Lipid Particles 0.036028 0.012047 0.003431 0.006343 0.025913 0.017732 0.005942 0.004101 0.030374 0.016169 0.003955 0.004301
Mitochondria 0.163514* 0.141524* 0.002790 0.000649 0.247150* 0.329564* 0.004380 0.004734 0.210259* 0.277859* 0.003122 0.004369
Mitotic Spindle 0.003301 0.020769 0.013810 0.019053 0.005441 0.011587 0.029491 0.013256 0.004497 0.014112 0.017082 0.013774
None 0.001333 0.002979 0.004164 0.004373 0.003201 0.001191 0.002295 0.002473 0.002377 0.001682 0.003774 0.002643
Nuclear Periphery 0.001144 0.000374 0.002960 0.001808 0.000480 0.000285 0.001834 0.000762 0.000773 0.000310 0.002725 0.000856
Nuclear Periphery Foci 0.000466 0.000520 0.001865 0.006736 0.000312 0.000266 0.002570 0.001132 0.000380 0.000336 0.002012 0.001633
Nucleolus 0.002318 0.003535 0.004069 0.001041 0.002856 0.002178 0.002909 0.001232 0.002619 0.002551 0.003827 0.001215
Nucleus 0.043349 0.036824 0.335418* 0.234721* 0.020641 0.013845 0.226818* 0.195322* 0.030657 0.020163 0.312753* 0.198843*
Peroxisomes 0.034022 0.069814 0.003741 0.012341 0.048798 0.063317 0.027746 0.030504 0.042280 0.065104 0.008751 0.028881
Vacuole 0.061111 0.066533 0.077398 0.259252* 0.041116 0.041516 0.159509 0.130698 0.049935 0.048395 0.094535 0.142184
Vacuole Periphery 0.011294 0.011269 0.002067 0.000357 0.013468 0.014109 0.001772 0.001169 0.012509 0.013328 0.002005 0.001096

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.19 0.52 -1.40 -1.04 -1.41 -2.04 -2.12 -4.12 -3.68 -1.30 -3.04 -1.64 -4.37 -3.45 -3.92
Bud Neck -2.93 11.00 10.16 10.03 1.73 -0.84 0.81 4.58 5.02 2.34 -1.49 11.19 8.37 8.71 -1.73
Bud Site -7.68 3.96 -1.62 1.32 -2.28 -1.46 -4.93 -6.12 -5.22 -0.19 -6.24 1.27 -6.60 -3.01 -7.06
Cell Periphery 3.43 8.48 -1.10 -2.66 -4.58 -2.04 2.74 0.49 2.29 -1.88 -1.20 9.46 0.79 1.63 -5.46
Cytoplasm 1.70 -33.00 -3.78 -4.37 9.01 7.21 -15.96 -31.23 -36.61 -6.48 8.69 -41.68 -29.32 -34.18 3.50
Cytoplasmic Foci 4.76 36.88 25.82 18.52 1.43 11.09 20.63 29.37 18.74 2.61 13.03 46.52 38.55 24.39 -2.96
Eisosomes 4.56 14.75 0.07 -0.97 -2.58 -0.27 9.02 5.70 3.08 -3.38 0.90 18.95 6.37 2.83 -8.29
Endoplasmic Reticulum 2.36 -1.46 -5.05 -7.19 -4.85 3.32 -6.20 -1.73 -10.47 6.09 4.34 -4.70 -2.13 -11.28 3.65
Endosome 0.06 18.96 19.73 15.64 2.71 -1.24 18.81 24.47 25.64 4.78 -1.62 29.00 30.65 29.48 4.06
Golgi -2.22 15.55 15.93 12.43 3.72 -4.32 23.43 22.13 24.45 -2.16 -6.81 27.70 25.21 26.42 -2.34
Lipid Particles 9.28 14.37 13.12 4.76 -3.44 4.03 11.70 13.25 11.52 3.44 8.63 19.24 19.10 12.75 -0.42
Mitochondria 2.23 24.36 24.71 15.87 8.92 -7.99 33.97* 33.97* 43.19* 0.33 -8.43 41.43* 41.14* 43.92* -0.60
Mitotic Spindle -6.53 -5.79 -1.93 1.24 0.22 -3.84 -4.99 -2.74 0.26 3.47 -7.22 -7.05 -3.69 1.41 2.55
None -1.36 -5.58 -1.80 -0.26 0.53 2.48 1.22 1.22 -2.97 -0.09 1.27 -2.17 0.17 -1.47 3.08
Nuclear Periphery 2.58 -5.86 -3.08 -6.80 2.08 1.96 -8.78 -4.88 -9.30 6.24 3.25 -10.83 -2.50 -11.09 11.10
Nuclear Periphery Foci -0.78 -7.89 -6.16 -6.06 -4.43 0.97 -5.90 -6.21 -6.46 3.42 0.60 -10.53 -7.83 -8.09 2.14
Nucleolus -2.03 -3.33 3.21 4.27 6.36 1.45 -0.04 3.12 2.51 2.98 0.12 -2.78 3.68 4.02 6.04
Nucleus 1.13 -42.62 -8.96 -9.14 4.85 3.00 -18.62 -29.23 -30.40 2.09 4.73 -48.92 -29.32 -30.93 12.50
Peroxisomes -7.15 14.29 8.79 12.39 -1.00 -3.87 5.85 6.99 10.52 -0.01 -7.80 18.35 7.02 12.98 -6.10
Vacuole -1.23 -6.13 -11.67 -11.36 -10.72 -0.20 -15.79 -21.97 -21.63 0.11 0.43 -16.09 -23.24 -23.09 -13.49
Vacuole Periphery 0.15 13.31 16.67 9.27 6.64 -0.64 16.98 17.29 16.56 2.05 -0.98 20.51 21.99 18.42 3.44
Standard name
Human Ortholog
Description Mitochondrial transporter; major substrates are adenosine 5'-phosphosulfate (APS) and 3'-phospho-adenosine 5'-phosphosulfate (PAPS); member of the mitochondrial carrier family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
Localization
Cell Percentages mitochondrion (65%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Mrx21

Mrx21


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrx21-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available