Standard name
Human Ortholog
Description Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.92 0.96 0.89 0.87 – 0.66 0.6 0.56 0.62 0.61 0.98 0.99 0.98 0.91 0.92 0.88 0.96 0.91 0.89 0.91 0.91 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.05 0 0.06 0.16 – 0.29 0.4 0.39 0.38 0.43 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.05 0 0.07 0.06 – 0.2 0.11 0.17 0.08 0 0 0 0 0.15 0.12 0.21 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0
Bud 0 0 2 1 1 1 – 0 1 1 2 0 0 0 0 0 0 1 1 0 1 2 2 1
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 2 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 4 – 0 2 1 2 5 0 0 0 5 4 5 0 0 0 0 0 0
Cytoplasm 246 163 119 24 97 123 – 124 103 95 119 102 238 313 279 320 290 333 241 149 118 157 265 246
Endoplasmic Reticulum 0 2 0 0 0 0 – 0 0 1 3 3 2 4 0 1 4 3 0 0 0 1 2 0
Endosome 1 0 0 0 0 0 – 0 0 2 0 0 0 0 0 3 1 1 0 0 0 0 0 0
Golgi 1 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 3 2 1 5 1 0 2 0
Mitochondria 1 0 7 1 6 23 – 54 69 66 73 72 0 0 1 2 5 0 1 0 1 0 2 1
Nucleus 1 0 1 0 2 0 – 5 4 12 7 3 1 1 4 0 4 1 1 1 0 2 1 1
Nuclear Periphery 0 0 0 0 0 0 – 2 2 0 2 0 0 0 0 0 0 1 0 1 1 0 0 0
Nucleolus 0 0 1 0 1 0 – 0 0 0 1 0 1 0 0 0 2 0 0 0 1 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 3 7 1 8 8 – 37 18 29 15 6 4 0 4 52 39 80 0 2 0 5 5 4
Unique Cell Count 249 166 130 25 109 141 188 171 170 192 168 242 317 285 350 316 378 252 164 133 173 291 263
Labelled Cell Count 252 168 137 27 115 159 224 199 207 224 191 246 318 289 383 352 427 252 164 133 173 291 263


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.0 10.0 6.5 7.9 8.2 7.2 – 6.9 6.0 6.1 5.8 5.7 9.6 9.7 9.4 16.1 14.6 14.9 9.1 9.5 9.3
Std Deviation (1e-4) 1.3 1.8 1.8 1.7 2.0 2.1 – 2.1 1.6 1.5 1.4 1.4 1.7 1.6 1.6 4.0 3.5 3.5 1.8 1.8 1.8
Intensity Change (Log2) – – – 0.27 0.32 0.14 – 0.07 -0.12 -0.1 -0.17 -0.2 0.55 0.57 0.53 1.3 1.16 1.19 0.47 0.54 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 -0.7 -1.1 – -5.3 -6.1 -6.8 -5.9 -6.0 3.2 3.8 3.0 0 0.1 -1.1
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.9 – 5.2 6.9 6.7 6.6 7.3 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 2.7 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0.6 0.1 – 3.6 1.6 3.1 0.8 -0.8 0 0 0 2.8 2.2 4.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5031 3.7165 3.4346 3.1831 3.1325 3.6684 3.3461 4.2418 3.6694 3.4991 3.8362 3.5424 4.2012 5.2958 4.8013 4.9064 4.4492 4.2219
Actin 0.0097 0.0033 0.0118 0.0283 0.0082 0.0042 0.0251 0.0052 0.0149 0.0546 0.0003 0.0091 0.0028 0.0002 0.0058 0.0005 0.0003 0.0002
Bud 0.0002 0.0002 0.0005 0.0002 0.0005 0.0011 0.0049 0.0106 0.004 0.0122 0.0001 0.0051 0.0003 0.0001 0.0001 0.0002 0.0001 0.0001
Bud Neck 0.0004 0.0002 0.0057 0.0013 0.0006 0.0024 0.0013 0.0005 0.0071 0.0014 0.0006 0.0119 0.0073 0.0001 0.0004 0.0006 0.0006 0.0008
Bud Periphery 0.0001 0.0001 0.0004 0.0002 0.0004 0.0007 0.003 0.0027 0.0017 0.0083 0 0.0051 0.0003 0 0.0001 0 0.0001 0
Bud Site 0.0007 0.0012 0.0043 0.0005 0.0009 0.0006 0.0058 0.0187 0.0076 0.0041 0.0001 0.0031 0.0059 0.0003 0.0004 0.0001 0.0002 0.0002
Cell Periphery 0.0001 0.0001 0.0002 0 0 0 0.0003 0.0002 0.0002 0.0003 0 0.0002 0.0001 0 0 0 0 0
Cytoplasm 0.8092 0.8912 0.8245 0.8404 0.7933 0.8976 0.7466 0.8698 0.835 0.721 0.9552 0.8539 0.8178 0.9373 0.856 0.9082 0.9059 0.9317
Cytoplasmic Foci 0.0165 0.004 0.0222 0.0135 0.0154 0.0161 0.0448 0.0055 0.0126 0.0275 0.0087 0.0206 0.0114 0.0038 0.0056 0.0063 0.0021 0.0054
Eisosomes 0 0.0001 0.0001 0.0003 0.0001 0 0.0001 0 0.0001 0.0002 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0047 0.0024 0.0032 0.0019 0.0023 0.0021 0.0048 0.0016 0.0032 0.0036 0.0014 0.0035 0.005 0.0013 0.0028 0.0037 0.0026 0.0019
Endosome 0.0174 0.0037 0.0226 0.0071 0.0116 0.0076 0.0213 0.001 0.0094 0.0217 0.0036 0.0051 0.0181 0.0008 0.0033 0.0035 0.0092 0.0049
Golgi 0.0046 0.0002 0.0101 0.0177 0.017 0.0023 0.0066 0.0004 0.0037 0.0182 0.0002 0.001 0.0022 0.0001 0.0005 0.0001 0.0002 0.0003
Lipid Particles 0.0165 0.0002 0.0049 0.0056 0.017 0.0029 0.012 0.0001 0.0013 0.0096 0 0.0003 0.0026 0 0.0006 0.0001 0.0002 0
Mitochondria 0.0073 0.0088 0.008 0.0018 0.0331 0.0007 0.0028 0.0004 0.0113 0.0088 0.0002 0.0011 0.0013 0.0001 0.0004 0.0002 0.0009 0.0003
None 0.0821 0.0688 0.0434 0.0084 0.0201 0.0345 0.0676 0.058 0.0532 0.0472 0.0105 0.0309 0.0713 0.0229 0.0771 0.0521 0.0053 0.0143
Nuclear Periphery 0.0047 0.0015 0.0029 0.0021 0.0028 0.0019 0.0057 0.0017 0.0037 0.0051 0.0015 0.003 0.0148 0.0011 0.0033 0.0021 0.0134 0.0021
Nucleolus 0.0006 0 0.0005 0.0002 0.0002 0.0001 0.0004 0.0002 0.0004 0.0004 0 0.0008 0.0007 0.0001 0.0001 0 0.001 0
Nucleus 0.0112 0.0111 0.0203 0.0556 0.0163 0.0185 0.017 0.0132 0.0206 0.021 0.0152 0.0352 0.0271 0.0301 0.0375 0.0196 0.0508 0.0352
Peroxisomes 0.0076 0.0012 0.0082 0.0109 0.0543 0.0031 0.0089 0.0058 0.0025 0.0173 0.0002 0.0015 0.0033 0 0.0004 0.0001 0.0001 0.0001
Punctate Nuclear 0.004 0.0007 0.0026 0.0032 0.0042 0.0026 0.016 0.0038 0.0053 0.0153 0.0015 0.0073 0.0059 0.0013 0.0045 0.0015 0.0038 0.0014
Vacuole 0.0019 0.0011 0.0027 0.0005 0.0012 0.001 0.0041 0.0006 0.0017 0.0016 0.0005 0.0011 0.0014 0.0003 0.0006 0.0009 0.0023 0.0008
Vacuole Periphery 0.0005 0.0002 0.0006 0.0002 0.0004 0.0002 0.0008 0.0001 0.0006 0.0007 0.0001 0.0003 0.0006 0 0.0002 0.0001 0.0008 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 44.2724 42.6212 34.6388 63.7417 52.9856 34.0368 58.9981 65.7891 56.7536 79.0616
Translational Efficiency 2.2036 2.3977 2.7027 1.3232 1.8146 3.0724 2.1115 1.7811 2.3862 1.9372

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1646 1450 860 1503 2113 2083 255 1511 3759 3533 1115 3014

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 792.13 896.87 1203.26 1145.85 864.79 935.37 1292.15 1147.60 832.97 919.57 1223.59 1146.73
Standard Deviation 131.47 145.64 201.32 193.34 116.71 126.74 167.11 184.40 128.55 136.14 197.59 188.91
Intensity Change Log 2 — 0.179162 0.603139 0.532609 — 0.113187 0.579352 0.408198 — 0.145105 0.590773 0.469016

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000065 0.000321 0.000262 0.002929 0.000132 0.000159 0.002637 0.000736 0.000103 0.000225 0.000805 0.001830
Bud Neck 0.001255 0.005971 0.001408 0.007239 0.002618 0.005039 0.001898 0.008605 0.002021 0.005422 0.001520 0.007924
Bud Site 0.002181 0.013370 0.004504 0.021142 0.004494 0.009088 0.009233 0.043180 0.003481 0.010845 0.005585 0.032190
Cell Periphery 0.000085 0.000101 0.000188 0.000204 0.000105 0.000067 0.000134 0.000078 0.000096 0.000081 0.000175 0.000141
Cytoplasm 0.743800* 0.697809* 0.511397* 0.568344* 0.719885* 0.703895* 0.446087* 0.604465* 0.730357* 0.701397* 0.496460* 0.586452*
Cytoplasmic Foci 0.063540 0.096408 0.011398 0.017817 0.074887 0.076123 0.005728 0.015492 0.069918 0.084448 0.010101 0.016652
Eisosomes 0.000028 0.000036 0.000041 0.000039 0.000023 0.000030 0.000026 0.000016 0.000025 0.000032 0.000037 0.000028
Endoplasmic Reticulum 0.000590 0.000893 0.003995 0.001657 0.000626 0.000784 0.002892 0.000496 0.000611 0.000829 0.003743 0.001075
Endosome 0.003460 0.008073 0.002525 0.012322 0.002470 0.007275 0.001796 0.010075 0.002903 0.007602 0.002358 0.011196
Golgi 0.000914 0.002236 0.000394 0.010988 0.000936 0.001831 0.000149 0.008783 0.000926 0.001997 0.000338 0.009883
Lipid Particles 0.001355 0.002049 0.002379 0.001596 0.000868 0.001644 0.001173 0.000387 0.001081 0.001810 0.002103 0.000990
Mitochondria 0.001170 0.003111 0.000256 0.006777 0.001447 0.002569 0.000285 0.005625 0.001325 0.002791 0.000263 0.006200
Mitotic Spindle 0.000400 0.011934 0.000489 0.081518 0.002278 0.008100 0.007128 0.088488 0.001456 0.009674 0.002007 0.085012
None 0.017124 0.004937 0.011877 0.009134 0.004195 0.003909 0.012968 0.010443 0.009856 0.004331 0.012127 0.009790
Nuclear Periphery 0.000254 0.000237 0.000890 0.001320 0.000181 0.000386 0.001081 0.000478 0.000213 0.000325 0.000934 0.000898
Nuclear Periphery Foci 0.002104 0.002104 0.002948 0.002382 0.000879 0.000726 0.004341 0.001110 0.001415 0.001292 0.003266 0.001744
Nucleolus 0.001314 0.001187 0.000482 0.000559 0.000339 0.001151 0.000142 0.000315 0.000766 0.001166 0.000404 0.000437
Nucleus 0.123004 0.073208 0.345611* 0.133185* 0.134018* 0.108039 0.366386* 0.119491 0.129195 0.093744 0.350362* 0.126320
Peroxisomes 0.000387 0.002861 0.000225 0.001438 0.000567 0.002368 0.000553 0.001524 0.000488 0.002571 0.000300 0.001481
Vacuole 0.036525 0.072081 0.098566 0.114100 0.048564 0.066096 0.135264 0.072197 0.043292 0.068552 0.106959 0.093093
Vacuole Periphery 0.000447 0.001074 0.000167 0.005311 0.000486 0.000722 0.000099 0.008014 0.000469 0.000866 0.000151 0.006666

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.42 -9.70 -2.79 -1.79 -1.59 -0.73 -1.39 -3.81 -3.72 1.18 -4.24 -2.29 -3.52 -2.58 0.29
Bud Neck -6.87 -3.29 -5.10 2.93 -3.37 -3.98 -0.44 -1.36 2.18 -1.20 -7.54 -0.59 -3.78 3.57 -3.65
Bud Site -6.64 -1.91 -6.53 1.93 -3.15 -3.42 -1.15 -4.51 -1.67 -2.34 -7.04 -1.49 -7.16 -0.13 -4.22
Cell Periphery -1.53 -8.37 -2.14 -0.60 5.60 2.16 -2.95 2.23 -0.05 9.30 1.44 -6.55 0.81 -1.01 9.50
Cytoplasm 6.51 27.76 31.39 22.14 -1.74 2.49 28.82 24.72 21.07 -12.86 6.20 35.87 39.51 30.96 -7.60
Cytoplasmic Foci -7.30 19.67 20.44 24.73 1.66 0.04 27.54 26.62 24.94 -3.08 -4.80 32.28 33.46 34.96 1.06
Eisosomes -3.01 -4.62 0.36 3.36 4.92 -4.06 -4.14 3.65 7.40 5.92 -4.74 -6.69 1.56 6.18 7.64
Endoplasmic Reticulum -2.53 -4.59 -5.48 -1.40 3.90 -0.55 -5.18 -0.08 0.56 5.30 -1.36 -5.41 -3.33 -0.27 4.99
Endosome -5.84 2.37 1.80 7.04 -0.00 -7.25 3.25 1.12 6.84 -0.56 -9.19 2.43 1.99 9.59 0.23
Golgi -2.22 1.61 -1.17 0.59 -2.23 -1.61 2.21 -0.69 0.67 -2.39 -2.63 2.29 -1.33 0.82 -3.02
Lipid Particles -0.99 -1.03 0.14 1.18 1.19 -1.53 -5.46 1.03 1.71 6.92 -1.75 -1.73 0.21 1.93 1.85
Mitochondria -1.66 1.82 -0.41 1.48 -2.92 -1.44 2.78 0.52 1.91 -2.97 -2.21 3.35 0.03 2.34 -4.19
Mitotic Spindle -5.29 -1.74 -10.05 -5.35 -9.94 -3.60 -1.72 -8.21 -5.88 -6.80 -6.30 -0.53 -12.83 -8.14 -12.76
None 6.50 3.50 5.52 -2.01 1.52 0.60 -1.96 -6.00 -7.03 -1.71 6.33 0.92 2.36 -5.98 0.74
Nuclear Periphery 0.12 -4.88 -13.51 -17.27 -3.25 -1.58 -15.47 -25.48 -5.84 5.38 -1.39 -8.33 -23.16 -11.98 -1.34
Nuclear Periphery Foci 0.06 -2.86 1.38 1.67 4.60 1.59 -7.12 -4.50 -5.93 6.10 0.92 -6.96 -0.77 -2.19 7.04
Nucleolus 0.40 4.12 4.83 3.07 1.87 -2.87 3.39 1.79 3.23 -2.52 -1.70 3.74 5.08 4.40 1.99
Nucleus 11.13 -27.42 -9.13 -19.64 21.40 6.06 -15.92 -10.56 -15.54 12.10 11.32 -31.73 -13.75 -23.66 24.32
Peroxisomes -6.76 3.28 1.13 6.99 -1.71 -4.54 3.00 2.17 5.13 -0.78 -7.47 5.11 2.53 8.09 -1.81
Vacuole -11.40 -13.76 -33.56 -25.50 -14.56 -5.96 -11.93 -25.83 -22.16 1.21 -12.04 -17.13 -41.73 -33.98 -11.06
Vacuole Periphery -2.42 2.14 -1.43 0.42 -2.64 -1.35 2.91 -1.40 -0.99 -2.17 -2.69 3.43 -1.92 -0.70 -3.12
Standard name
Human Ortholog
Description Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine
Localization
Cell Percentages cytoplasm (92%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ltp1

Ltp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ltp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available