Standard name | |
---|---|
Human Ortholog | |
Description | Mitochondrial protein; required for normal mitochondrial morphology and inheritance; component of the mitochondria-ER-cortex-ancor (MECA); interacts with Num1p to link the ER and mitochondria at the cell cortex; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 41 | 86 | 12 | 11 | 11 | 1 | 14 | – | 6 | 7 | 2 | 3 | 29 | 46 | 48 | 119 | 102 | 92 | 73 | 79 | 18 | 14 | 38 | 51 |
Bud | 21 | 16 | 0 | 7 | 2 | 3 | 7 | – | 3 | 9 | 5 | 3 | 7 | 3 | 2 | 17 | 8 | 7 | 4 | 3 | 0 | 0 | 2 | 3 |
Bud Neck | 1 | 0 | 1 | 0 | 1 | 1 | 2 | – | 0 | 1 | 0 | 0 | 0 | 2 | 2 | 0 | 2 | 0 | 21 | 30 | 3 | 9 | 12 | 15 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | – | – | – | – | – | – |
Cell Periphery | 35 | 78 | 15 | 2 | 7 | 4 | 4 | – | 0 | 4 | 1 | 0 | 61 | 111 | 111 | 86 | 75 | 110 | 93 | 101 | 29 | 38 | 84 | 91 |
Cytoplasm | 188 | 159 | 48 | 40 | 63 | 99 | 124 | – | 16 | 87 | 40 | 42 | 243 | 546 | 664 | 87 | 89 | 105 | 58 | 50 | 16 | 55 | 60 | 77 |
Endoplasmic Reticulum | 7 | 20 | 4 | 1 | 3 | 6 | 16 | – | 2 | 16 | 6 | 5 | 7 | 7 | 5 | 24 | 21 | 22 | 64 | 51 | 12 | 9 | 19 | 27 |
Endosome | 4 | 2 | 0 | 0 | 0 | 1 | 1 | – | 0 | 2 | 0 | 0 | 1 | 5 | 3 | 2 | 6 | 1 | 3 | 5 | 2 | 2 | 1 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 7 | 3 | 1 | 3 | 0 | 0 | 0 | 0 |
Mitochondria | 40 | 6 | 0 | 9 | 1 | 11 | 24 | – | 28 | 53 | 81 | 53 | 0 | 0 | 1 | 16 | 12 | 8 | 61 | 47 | 39 | 36 | 35 | 36 |
Nucleus | 4 | 2 | 0 | 4 | 0 | 1 | 1 | – | 0 | 2 | 0 | 1 | 0 | 2 | 4 | 3 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 7 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 7 | 2 | 4 | 21 | 3 | 2 | 1 | 0 | 0 | 0 | 0 |
SpindlePole | 14 | 3 | 1 | 0 | 3 | 3 | 4 | – | 1 | 2 | 2 | 1 | 7 | 18 | 18 | 20 | 28 | 23 | 0 | 0 | 0 | 2 | 1 | 1 |
Vac/Vac Membrane | 1 | 2 | 0 | 0 | 1 | 2 | 0 | – | 0 | 1 | 0 | 0 | 2 | 0 | 2 | 3 | 11 | 6 | 12 | 14 | 2 | 7 | 9 | 12 |
Unique Cell Count | 299 | 289 | 76 | 55 | 82 | 119 | 174 | 43 | 150 | 114 | 84 | 295 | 632 | 731 | 286 | 289 | 302 | 403 | 393 | 130 | 182 | 272 | 325 | |
Labelled Cell Count | 364 | 376 | 81 | 75 | 92 | 132 | 197 | 56 | 184 | 137 | 108 | 358 | 748 | 862 | 385 | 385 | 381 | 403 | 393 | 130 | 182 | 272 | 325 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 6.0 | 6.0 | 5.2 | 6.2 | 5.0 | 4.9 | – | 4.0 | 4.6 | 4.1 | 4.4 | 6.4 | 6.3 | 6.2 | 7.5 | 7.8 | 7.2 | 6.0 | 6.1 | 6.2 |
Std Deviation (1e-4) | 0.8 | 0.7 | 0.7 | 1.0 | 1.3 | 1.6 | 0.9 | – | 0.8 | 1.0 | 1.2 | 0.8 | 1.2 | 1.2 | 1.1 | 1.1 | 1.4 | 1.1 | 1.3 | 1.3 | 1.1 |
Intensity Change (Log2) | – | – | – | -0.22 | 0.04 | -0.26 | -0.3 | – | -0.6 | -0.38 | -0.56 | -0.47 | 0.09 | 0.05 | 0.02 | 0.31 | 0.36 | 0.25 | -0.02 | 0.01 | 0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.6 | -0.4 | -4.1 | -1.8 | – | 0 | 0 | 0 | 0 | -1.5 | -2.6 | -2.9 | 4.2 | 3.3 | 2.6 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.7 | -2.0 | -3.8 | -4.8 | – | 0 | 0 | 0 | 0 | 0.2 | -0.5 | -1.0 | 1.8 | 1.1 | 2.8 |
Cytoplasm | 1.2 | 1.9 | 3.2 | 1.3 | – | -2.7 | -0.7 | -3.8 | -1.7 | 3.6 | 5.2 | 7.1 | -5.2 | -5.2 | -4.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 1.1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.9 | 0.6 | 0.6 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 3.4 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.7353 | 0.9176 | 0.8628 | 0.4102 | 0.6817 | 0.7612 | 1.227 | 1.1859 | 1.3621 | 0.9971 | 1.0141 | 1.0666 | 1.7622 | 1.9038 | 1.8354 | 1.9232 | 1.857 | 1.8056 |
Actin | 0.0989 | 0.0587 | 0.0988 | 0.1124 | 0.1401 | 0.0562 | 0.0829 | 0.0983 | 0.0528 | 0.0792 | 0.0773 | 0.0612 | 0.103 | 0.0453 | 0.0774 | 0.1001 | 0.012 | 0.0669 |
Bud | 0.006 | 0.0034 | 0.0056 | 0.0038 | 0.0086 | 0.0042 | 0.0031 | 0.0033 | 0.0038 | 0.0028 | 0.0126 | 0.0044 | 0.004 | 0.0052 | 0.0066 | 0.0032 | 0.0155 | 0.0059 |
Bud Neck | 0.0334 | 0.0069 | 0.0257 | 0.0213 | 0.0575 | 0.0338 | 0.023 | 0.0098 | 0.0192 | 0.0244 | 0.0306 | 0.0227 | 0.0242 | 0.0051 | 0.0342 | 0.0222 | 0.0054 | 0.03 |
Bud Periphery | 0.0289 | 0.0077 | 0.0168 | 0.0261 | 0.0347 | 0.0315 | 0.0119 | 0.0126 | 0.021 | 0.023 | 0.0341 | 0.037 | 0.0165 | 0.0062 | 0.0167 | 0.011 | 0.0472 | 0.0313 |
Bud Site | 0.063 | 0.144 | 0.0518 | 0.0227 | 0.0189 | 0.0134 | 0.0282 | 0.1838 | 0.0621 | 0.0219 | 0.0375 | 0.0082 | 0.0354 | 0.168 | 0.0744 | 0.0713 | 0.0212 | 0.0147 |
Cell Periphery | 0.0866 | 0.035 | 0.056 | 0.0196 | 0.0406 | 0.0563 | 0.0843 | 0.0656 | 0.074 | 0.103 | 0.0557 | 0.0808 | 0.0588 | 0.05 | 0.0513 | 0.0477 | 0.0153 | 0.0732 |
Cytoplasm | 0.2882 | 0.4155 | 0.3364 | 0.3113 | 0.258 | 0.4446 | 0.3814 | 0.3744 | 0.4863 | 0.4491 | 0.3436 | 0.4851 | 0.4198 | 0.5092 | 0.4857 | 0.4572 | 0.4344 | 0.533 |
Cytoplasmic Foci | 0.0359 | 0.0378 | 0.0354 | 0.106 | 0.0602 | 0.0461 | 0.0305 | 0.0222 | 0.022 | 0.014 | 0.0517 | 0.0312 | 0.048 | 0.0318 | 0.0417 | 0.044 | 0.1187 | 0.0344 |
Eisosomes | 0.0186 | 0.01 | 0.0102 | 0.0048 | 0.0059 | 0.0036 | 0.0164 | 0.0136 | 0.009 | 0.0193 | 0.0065 | 0.0121 | 0.0165 | 0.0058 | 0.0109 | 0.0049 | 0.0012 | 0.0099 |
Endoplasmic Reticulum | 0.0105 | 0.0102 | 0.0099 | 0.0056 | 0.0098 | 0.0062 | 0.0135 | 0.0045 | 0.006 | 0.0227 | 0.0049 | 0.0081 | 0.0067 | 0.0073 | 0.0089 | 0.0126 | 0.0036 | 0.005 |
Endosome | 0.0228 | 0.0304 | 0.0204 | 0.0355 | 0.0257 | 0.016 | 0.0189 | 0.005 | 0.007 | 0.0062 | 0.0391 | 0.008 | 0.0167 | 0.0102 | 0.0094 | 0.018 | 0.032 | 0.0068 |
Golgi | 0.0141 | 0.0225 | 0.0146 | 0.0311 | 0.0274 | 0.01 | 0.0104 | 0.0115 | 0.0075 | 0.0116 | 0.0175 | 0.0074 | 0.0138 | 0.0134 | 0.009 | 0.008 | 0.0059 | 0.0085 |
Lipid Particles | 0.0123 | 0.0031 | 0.0055 | 0.0077 | 0.0199 | 0.0053 | 0.0068 | 0.0017 | 0.0029 | 0.0192 | 0.0283 | 0.0031 | 0.0081 | 0.0017 | 0.0052 | 0.0031 | 0.0331 | 0.0102 |
Mitochondria | 0.0104 | 0.0069 | 0.014 | 0.0047 | 0.0407 | 0.0108 | 0.0074 | 0.0098 | 0.0103 | 0.0185 | 0.0153 | 0.0086 | 0.0101 | 0.0075 | 0.0177 | 0.0018 | 0.0081 | 0.0114 |
None | 0.2139 | 0.197 | 0.2691 | 0.2654 | 0.1794 | 0.2456 | 0.2511 | 0.174 | 0.197 | 0.1635 | 0.1821 | 0.2056 | 0.1774 | 0.1242 | 0.1164 | 0.1378 | 0.1573 | 0.1363 |
Nuclear Periphery | 0.0037 | 0.0007 | 0.0021 | 0.0006 | 0.0146 | 0.0004 | 0.0036 | 0.0003 | 0.0012 | 0.0005 | 0.0037 | 0.0023 | 0.0095 | 0.0003 | 0.0008 | 0.0014 | 0.0023 | 0.0006 |
Nucleolus | 0.0098 | 0.0009 | 0.0017 | 0.0006 | 0.0057 | 0.0007 | 0.0031 | 0.0006 | 0.001 | 0.002 | 0.0041 | 0.0007 | 0.0038 | 0.0004 | 0.0047 | 0.0024 | 0.0085 | 0.001 |
Nucleus | 0.0061 | 0.0016 | 0.0025 | 0.0011 | 0.0081 | 0.0011 | 0.0052 | 0.001 | 0.0016 | 0.0015 | 0.006 | 0.0027 | 0.009 | 0.0008 | 0.0029 | 0.0364 | 0.0047 | 0.002 |
Peroxisomes | 0.0166 | 0.0019 | 0.0139 | 0.0148 | 0.0178 | 0.0065 | 0.0049 | 0.0047 | 0.0027 | 0.0127 | 0.0174 | 0.0036 | 0.0068 | 0.0043 | 0.0097 | 0.006 | 0.0094 | 0.0147 |
Punctate Nuclear | 0.0095 | 0.0011 | 0.0035 | 0.0013 | 0.0182 | 0.0012 | 0.0071 | 0.0005 | 0.0034 | 0.0005 | 0.0219 | 0.0033 | 0.002 | 0.0004 | 0.0037 | 0.0057 | 0.0318 | 0.0019 |
Vacuole | 0.0097 | 0.0041 | 0.005 | 0.0028 | 0.0055 | 0.0056 | 0.0055 | 0.0026 | 0.0085 | 0.004 | 0.009 | 0.0033 | 0.008 | 0.0026 | 0.0116 | 0.0049 | 0.0282 | 0.0019 |
Vacuole Periphery | 0.0012 | 0.0005 | 0.0012 | 0.0005 | 0.0026 | 0.0006 | 0.0008 | 0.0002 | 0.0005 | 0.0005 | 0.0012 | 0.0004 | 0.0018 | 0.0002 | 0.0011 | 0.0003 | 0.0041 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.8292 | 5.6688 | 4.6272 | 6.153 | 5.7553 | 5.7041 | 7.0214 | 7.5437 | 5.3513 | 7.1811 |
Translational Efficiency | 0.3476 | 0.6576 | 0.6573 | 0.5574 | 0.4729 | 0.945 | 0.6509 | 0.5873 | 0.6688 | 0.6652 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1609 | 1187 | 166 | 151 | 2077 | 1795 | 771 | 98 | 3686 | 2982 | 937 | 249 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 638.28 | 754.59 | 917.44 | 908.79 | 713.62 | 736.56 | 869.53 | 892.30 | 680.73 | 743.74 | 878.02 | 902.30 |
Standard Deviation | 68.25 | 110.78 | 108.73 | 115.72 | 89.09 | 95.79 | 91.89 | 109.85 | 88.89 | 102.40 | 96.83 | 113.73 |
Intensity Change Log 2 | — | 0.241504 | 0.523424 | 0.509758 | — | 0.045647 | 0.285080 | 0.322373 | — | 0.141437 | 0.402527 | 0.413882 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.009066 | 0.043558 | 0.019385 | 0.109502* | 0.021613 | 0.011261 | 0.006171 | 0.074855 | 0.016136 | 0.024117 | 0.008512 | 0.095866 |
Bud Neck | 0.043482 | 0.081542 | 0.007901 | 0.022531 | 0.059823 | 0.135289 | 0.005894 | 0.016925 | 0.052690 | 0.113895 | 0.006250 | 0.020324 |
Bud Site | 0.021489 | 0.099053 | 0.034372 | 0.157865* | 0.035172 | 0.074067 | 0.015045 | 0.053841 | 0.029199 | 0.084013 | 0.018469 | 0.116924* |
Cell Periphery | 0.006203 | 0.008763 | 0.005947 | 0.021317 | 0.006584 | 0.007721 | 0.002693 | 0.009193 | 0.006418 | 0.008136 | 0.003269 | 0.016545 |
Cytoplasm | 0.471291* | 0.238516* | 0.283261* | 0.230584* | 0.396265* | 0.268743* | 0.440857* | 0.251003* | 0.429015* | 0.256711* | 0.412937* | 0.238620* |
Cytoplasmic Foci | 0.185983* | 0.188839* | 0.161142 | 0.121662 | 0.165956 | 0.124028 | 0.147681 | 0.193973* | 0.174698 | 0.149826 | 0.150066 | 0.150122 |
Eisosomes | 0.030213 | 0.012321 | 0.002837 | 0.014803 | 0.028227 | 0.011317 | 0.002673 | 0.007217 | 0.029094 | 0.011717 | 0.002702 | 0.011817 |
Endoplasmic Reticulum | 0.006230 | 0.017670 | 0.059068 | 0.023460 | 0.014415 | 0.014634 | 0.006599 | 0.011938 | 0.010842 | 0.015842 | 0.015894 | 0.018925 |
Endosome | 0.009846 | 0.046877 | 0.016331 | 0.007468 | 0.017172 | 0.052527 | 0.009246 | 0.011542 | 0.013974 | 0.050278 | 0.010501 | 0.009072 |
Golgi | 0.035518 | 0.046261 | 0.004003 | 0.004901 | 0.039566 | 0.025299 | 0.001616 | 0.002820 | 0.037799 | 0.033643 | 0.002039 | 0.004082 |
Lipid Particles | 0.032810 | 0.023905 | 0.031552 | 0.011374 | 0.040736 | 0.029613 | 0.014494 | 0.012900 | 0.037276 | 0.027341 | 0.017516 | 0.011975 |
Mitochondria | 0.016672 | 0.045291 | 0.000913 | 0.000513 | 0.024394 | 0.047344 | 0.000272 | 0.003236 | 0.021023 | 0.046527 | 0.000385 | 0.001584 |
Mitotic Spindle | 0.000173 | 0.000678 | 0.000742 | 0.001514 | 0.000300 | 0.001509 | 0.000417 | 0.004272 | 0.000244 | 0.001178 | 0.000474 | 0.002600 |
None | 0.006895 | 0.001165 | 0.000532 | 0.001285 | 0.002611 | 0.001376 | 0.002189 | 0.001319 | 0.004481 | 0.001292 | 0.001895 | 0.001298 |
Nuclear Periphery | 0.000141 | 0.000212 | 0.001156 | 0.000150 | 0.000169 | 0.000256 | 0.000789 | 0.000289 | 0.000157 | 0.000238 | 0.000854 | 0.000204 |
Nuclear Periphery Foci | 0.000307 | 0.000590 | 0.017393 | 0.005098 | 0.000417 | 0.000423 | 0.004255 | 0.005822 | 0.000369 | 0.000489 | 0.006583 | 0.005383 |
Nucleolus | 0.000479 | 0.000363 | 0.000479 | 0.000095 | 0.000389 | 0.000943 | 0.000503 | 0.000177 | 0.000428 | 0.000712 | 0.000499 | 0.000127 |
Nucleus | 0.068907 | 0.024628 | 0.194568* | 0.081104 | 0.074986 | 0.039837 | 0.226973* | 0.051715 | 0.072332 | 0.033783 | 0.221233* | 0.069537 |
Peroxisomes | 0.030585 | 0.047378 | 0.006957 | 0.015190 | 0.040244 | 0.030765 | 0.005092 | 0.021769 | 0.036028 | 0.037378 | 0.005422 | 0.017779 |
Vacuole | 0.022636 | 0.065647 | 0.150949 | 0.169017 | 0.029251 | 0.115898 | 0.106345 | 0.264536* | 0.026363 | 0.095895 | 0.114247 | 0.206611* |
Vacuole Periphery | 0.001074 | 0.006746 | 0.000511 | 0.000567 | 0.001709 | 0.007150 | 0.000197 | 0.000658 | 0.001432 | 0.006989 | 0.000252 | 0.000603 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -10.09 | -2.18 | -6.10 | -3.80 | -5.25 | 5.50 | 8.12 | -2.51 | -3.17 | -3.51 | -4.49 | 5.05 | -6.24 | -5.51 | -6.93 |
Bud Neck | -8.33 | 15.97 | 5.07 | 10.91 | -3.97 | -14.86 | 21.68 | 10.99 | 21.78 | -2.58 | -17.13 | 27.34 | 10.98 | 23.30 | -5.09 |
Bud Site | -15.92 | -2.64 | -7.97 | -3.22 | -7.05 | -10.46 | 7.86 | -1.64 | 2.93 | -4.14 | -18.51 | 5.20 | -7.53 | -2.55 | -8.50 |
Cell Periphery | -2.62 | 0.56 | -4.36 | -3.45 | -4.43 | -1.35 | 7.58 | -0.44 | 0.06 | -2.22 | -2.71 | 8.24 | -4.05 | -3.19 | -5.58 |
Cytoplasm | 28.27 | 11.81 | 14.55 | 0.84 | 2.21 | 17.37 | -3.18 | 6.71 | 1.31 | 7.72 | 31.18 | 3.19 | 14.44 | 1.96 | 11.06 |
Cytoplasmic Foci | -0.43 | 2.26 | 5.35 | 5.41 | 2.29 | 8.54 | 2.82 | -0.63 | -2.69 | -1.52 | 6.19 | 4.45 | 2.83 | 0.57 | 0.47 |
Eisosomes | 8.90 | 14.89 | 5.35 | -0.56 | -4.28 | 10.63 | 17.70 | 10.39 | 2.80 | -1.94 | 13.84 | 22.59 | 9.21 | 0.53 | -4.67 |
Endoplasmic Reticulum | -6.41 | -8.04 | -5.93 | -1.78 | 4.96 | -0.11 | 6.15 | 1.06 | 1.10 | -1.98 | -4.11 | -3.34 | -3.81 | -1.36 | -1.16 |
Endosome | -14.25 | -3.16 | 1.21 | 12.81 | 3.37 | -14.31 | 5.82 | 3.01 | 14.24 | -0.64 | -20.12 | 3.22 | 3.53 | 19.46 | 1.21 |
Golgi | -3.71 | 18.04 | 16.62 | 16.22 | -0.63 | 7.34 | 26.43 | 21.33 | 13.77 | -0.70 | 2.54 | 32.98 | 26.77 | 20.83 | -2.30 |
Lipid Particles | 4.18 | 0.42 | 9.39 | 5.27 | 5.18 | 4.97 | 12.33 | 10.39 | 6.65 | 1.08 | 6.30 | 11.80 | 14.35 | 8.92 | 3.19 |
Mitochondria | -8.82 | 11.85 | 12.45 | 15.04 | 1.08 | -8.26 | 17.34 | 8.05 | 13.38 | -0.96 | -12.11 | 21.30 | 14.77 | 21.72 | -0.98 |
Mitotic Spindle | -2.31 | -2.34 | -1.82 | -0.98 | -1.02 | -3.47 | -0.33 | -1.04 | -0.64 | -1.01 | -4.20 | -1.17 | -1.60 | -0.83 | -1.42 |
None | 6.76 | 8.95 | 3.83 | -0.23 | -0.75 | 2.76 | 0.62 | 2.05 | 0.43 | 1.14 | 7.18 | 3.94 | 3.63 | -0.03 | 0.56 |
Nuclear Periphery | -1.84 | -10.64 | -0.63 | 1.47 | 10.47 | -3.83 | -11.31 | -3.21 | -1.61 | 6.01 | -3.92 | -14.44 | -2.84 | 0.27 | 11.85 |
Nuclear Periphery Foci | -3.90 | -7.49 | -6.59 | -6.18 | 5.09 | 0.05 | -10.46 | -4.30 | -4.30 | -1.24 | -2.36 | -11.91 | -7.51 | -7.35 | 1.34 |
Nucleolus | 2.14 | -0.15 | 9.85 | 5.95 | 6.29 | -6.40 | -1.77 | 5.05 | 9.24 | 4.62 | -4.99 | -1.49 | 10.58 | 11.18 | 6.25 |
Nucleus | 16.00 | -8.86 | -1.17 | -5.97 | 6.87 | 11.76 | -18.83 | 3.09 | -1.28 | 16.22 | 18.42 | -21.21 | 0.64 | -5.28 | 16.42 |
Peroxisomes | -6.13 | 13.88 | 5.28 | 9.35 | -2.81 | 4.06 | 20.34 | 3.35 | 1.93 | -1.94 | -0.75 | 24.62 | 6.27 | 6.48 | -3.43 |
Vacuole | -12.56 | -11.62 | -9.43 | -6.90 | -1.17 | -20.15 | -15.96 | -10.28 | -7.21 | -7.05 | -23.52 | -19.73 | -13.42 | -8.99 | -6.87 |
Vacuole Periphery | -8.76 | 2.78 | 1.42 | 8.36 | -0.08 | -11.03 | 10.35 | 2.98 | 11.05 | -0.90 | -14.17 | 12.11 | 3.18 | 13.82 | -1.03 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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