Standard name
Human Ortholog
Description Beta 5 subunit of the 20S proteasome; responsible for the chymotryptic activity of the proteasome
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.06 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Cytoplasm 0.89 0.97 0.96 0.97 0.96 0.92 0.92 – 0.77 0.85 0.72 0.76 1.0 1.0 1.0 0.78 – 0.91 0.78 0.82 0.66 0.83 0.8 0.79
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0.11 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Mitochondria 0.13 0 0 0 0 0.07 0.07 – 0.38 0.22 0.38 0.32 0 0 0 0.26 – 0 0.12 0.06 0.14 0 0.05 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.09 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 – 0 0 4 14 4 3 1
Bud 0 0 0 3 2 2 3 – 7 7 8 18 0 0 0 0 – 1 0 4 11 5 12 14
Bud Neck 0 3 20 12 18 14 22 – 4 4 7 12 0 1 1 0 – 0 2 2 6 1 2 3
Bud Site 0 0 0 0 0 1 0 – 1 0 1 1 0 0 0 0 – 0 – – – – – –
Cell Periphery 2 3 1 4 1 1 1 – 0 2 0 1 0 1 1 0 – 0 0 2 9 2 3 2
Cytoplasm 134 412 564 330 311 542 528 – 295 305 272 361 197 288 270 18 – 32 138 333 367 288 298 277
Endoplasmic Reticulum 0 2 1 0 1 0 0 – 0 0 0 1 0 0 0 0 – 4 0 0 4 3 4 4
Endosome 1 0 6 0 0 3 1 – 1 0 1 0 0 0 0 0 – 0 2 4 8 3 4 4
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 1 3 1 0 1 0
Mitochondria 19 13 0 3 12 44 43 – 146 77 144 151 0 0 0 6 – 1 21 26 79 15 19 15
Nucleus 0 1 1 1 3 1 4 – 2 1 1 5 1 0 0 0 – 1 0 1 0 1 2 2
Nuclear Periphery 0 0 1 0 0 0 1 – 1 0 0 0 0 0 0 1 – 0 0 1 0 0 0 2
Nucleolus 0 1 1 0 3 0 1 – 1 1 0 0 0 0 1 2 – 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 – 0 0 1 1 0 0 0
SpindlePole 1 0 7 8 2 16 16 – 10 6 16 24 0 0 0 0 – 0 2 4 19 9 4 8
Vac/Vac Membrane 2 2 5 1 1 2 5 – 1 2 3 2 0 0 0 0 – 0 3 7 4 3 4 4
Unique Cell Count 151 425 586 340 323 588 574 383 357 379 478 197 288 271 23 35 178 409 555 349 373 351
Labelled Cell Count 160 437 607 362 354 627 625 469 406 453 576 198 290 273 27 39 178 409 555 349 373 351


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 3.2 5.0 4.7 4.3 3.5 4.1 – 3.1 3.4 3.2 3.3 4.8 4.3 4.4 6.0 – 5.4 5.3 5.6 5.7
Std Deviation (1e-4) 0.6 0.8 2.0 2.1 1.9 1.0 1.7 – 1.3 1.2 0.9 1.1 1.4 1.0 1.2 3.2 – 3.1 1.7 2.1 2.0
Intensity Change (Log2) – – – -0.08 -0.19 -0.5 -0.28 – -0.7 -0.56 -0.61 -0.59 -0.04 -0.2 -0.16 0.28 – 0.12 0.09 0.17 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.481 3.4489 3.5508 3.1572 2.9995 3.3498 3.5263 2.9173 3.283 2.1771 3.1995 3.1493 0.4346 0.687 0.6404 1.0918 -0.0343 0.8079
Actin 0.0319 0.0015 0.0027 0.0044 0.0083 0.0088 0.0178 0.0002 0.0204 0.0003 0.0612 0.0014 0.015 0 0.0035 0.0028 0 0.0303
Bud 0.0008 0.0046 0.0022 0.0002 0.0052 0.0007 0.001 0.0007 0.0007 0.0041 0.0023 0.0007 0.0015 0 0.0002 0.0088 0 0.0025
Bud Neck 0.0011 0.0013 0.003 0.0003 0.0021 0.0003 0.0004 0.0006 0.0012 0.0001 0.0016 0.0002 0.0002 0 0.0016 0.0002 0 0.0012
Bud Periphery 0.0014 0.0025 0.009 0.0003 0.0192 0.003 0.0079 0.0009 0.002 0.0014 0.0041 0.0028 0.0044 0 0.0001 0.016 0 0.0084
Bud Site 0.0107 0.011 0.0091 0.0008 0.0201 0.0007 0.0014 0.0138 0.0055 0.0009 0.0199 0.0002 0.0035 0 0.0004 0.0009 0 0.0008
Cell Periphery 0.0008 0.0004 0.0009 0 0.0009 0 0.0009 0.0002 0.0008 0.0002 0.0004 0.0001 0.0001 0 0 0.0001 0 0.0001
Cytoplasm 0.0256 0.0285 0.0228 0.019 0.0493 0.0262 0.0269 0.0336 0.0304 0.0174 0.0094 0.0368 0.0135 0.0097 0.0096 0.0159 0.0258 0.0221
Cytoplasmic Foci 0.0392 0.0076 0.0124 0.0169 0.0383 0.0134 0.0168 0.0037 0.008 0.0131 0.0302 0.005 0.0123 0.0011 0.0171 0.0251 0.0009 0.0043
Eisosomes 0.0007 0.0002 0.0004 0.0001 0.0002 0.0001 0.0009 0.0001 0.0008 0.0001 0.0004 0.0001 0.0003 0.0001 0.0002 0.0002 0.0001 0.0002
Endoplasmic Reticulum 0.0068 0.0037 0.0016 0.0001 0.0006 0.0006 0.002 0.0002 0.0035 0.0003 0.0011 0.0006 0.0027 0.0001 0.0002 0.0013 0.0003 0.0005
Endosome 0.0229 0.0065 0.008 0.0059 0.0135 0.0101 0.0082 0.0023 0.0081 0.0002 0.0529 0.0116 0.0214 0 0.0037 0.0103 0.0001 0.0023
Golgi 0.008 0.0027 0.0054 0.0249 0.0118 0.0026 0.005 0.0009 0.0015 0.0001 0.0414 0.0032 0.0048 0 0.005 0.0019 0 0.0023
Lipid Particles 0.0141 0.0013 0.0034 0.0006 0.0031 0.0021 0.0115 0.0018 0.0028 0.0004 0.0142 0.0084 0.0081 0.0001 0.0137 0.0158 0.0001 0.0009
Mitochondria 0.0123 0.0008 0.0018 0.0632 0.0226 0.0023 0.0058 0.0007 0.0016 0.0009 0.0097 0.0008 0.0073 0 0.0007 0.0031 0 0.0026
None 0.7686 0.9131 0.9095 0.8529 0.7828 0.9176 0.8395 0.9348 0.8886 0.9544 0.7237 0.9185 0.8661 0.9884 0.9287 0.816 0.9724 0.9162
Nuclear Periphery 0.0086 0.0007 0.0008 0 0.0004 0.0015 0.0075 0.0001 0.0028 0.0003 0.0016 0.0016 0.0171 0 0.0002 0.0109 0.0001 0.0005
Nucleolus 0.0043 0.0007 0.0003 0 0.0005 0.0001 0.0003 0.0011 0.0003 0.001 0.0004 0.0003 0.0006 0 0.0005 0.002 0 0.0001
Nucleus 0.0113 0.001 0.0004 0.0001 0.0006 0.0003 0.0273 0.0005 0.0008 0.0013 0.0004 0.0015 0.0061 0 0.0004 0.0441 0.0001 0.0002
Peroxisomes 0.0136 0.0076 0.003 0.0097 0.0143 0.0066 0.0105 0.0014 0.0177 0.0005 0.0209 0.0029 0.0067 0 0.0094 0.0062 0 0.0035
Punctate Nuclear 0.0133 0.0032 0.0004 0.0002 0.0006 0.0017 0.0067 0.0003 0.0009 0.002 0.0022 0.0021 0.0051 0.0001 0.0045 0.0159 0 0.0005
Vacuole 0.0029 0.0006 0.002 0.0001 0.004 0.0006 0.0008 0.0019 0.0013 0.0007 0.0014 0.0009 0.0021 0.0001 0.0002 0.0015 0 0.0003
Vacuole Periphery 0.0011 0.0001 0.0008 0.0001 0.0016 0.0006 0.0009 0.0001 0.0004 0.0002 0.0008 0.0004 0.001 0 0.0001 0.0009 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 135.3714 110.739 106.7403 169.0048 104.3155 112.3216 138.2286 146.0778 146.8094 149.1005
Translational Efficiency 1.9981 2.0491 2.3783 1.4822 1.811 3.0161 2.0349 1.8831 1.798 1.5674

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1428 1910 1293 1887 1799 2082 145 2212 3227 3992 1438 4099

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 635.37 734.62 739.98 722.51 632.91 774.38 775.70 714.35 634.00 755.36 743.58 718.11
Standard Deviation 93.68 82.64 78.78 74.39 69.04 86.87 78.92 66.97 80.88 87.17 79.52 70.60
Intensity Change Log 2 — 0.209401 0.219889 0.185421 — 0.291041 0.293498 0.174631 — 0.250720 0.257092 0.180046

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000065 0.000216 0.000172 0.000091 0.000064 0.000222 0.000101 0.000034 0.000064 0.000219 0.000165 0.000060
Bud Neck 0.019282 0.029287 0.003060 0.004910 0.009400 0.028724 0.000788 0.003527 0.013773 0.028994 0.002831 0.004164
Bud Site 0.002565 0.011438 0.013364 0.008278 0.003717 0.010833 0.000459 0.006300 0.003207 0.011122 0.012063 0.007211
Cell Periphery 0.000573 0.000101 0.000151 0.000110 0.000161 0.000191 0.000317 0.000140 0.000343 0.000148 0.000168 0.000126
Cytoplasm 0.414879* 0.448368* 0.468369* 0.503240* 0.518932* 0.479422* 0.298301* 0.532556* 0.472887* 0.464564* 0.451220* 0.519060*
Cytoplasmic Foci 0.202120* 0.205916* 0.020606 0.018499 0.145441 0.139663 0.080649 0.031234 0.170522 0.171362 0.026660 0.025371
Eisosomes 0.000551 0.000097 0.000032 0.000027 0.000127 0.000132 0.000168 0.000038 0.000315 0.000116 0.000046 0.000033
Endoplasmic Reticulum 0.002420 0.000862 0.000684 0.000400 0.000536 0.000501 0.000937 0.000484 0.001370 0.000674 0.000710 0.000445
Endosome 0.011354 0.017962 0.001711 0.001541 0.004476 0.010485 0.005472 0.001878 0.007519 0.014062 0.002090 0.001723
Golgi 0.002874 0.003303 0.000058 0.000060 0.000888 0.002092 0.000727 0.000171 0.001767 0.002672 0.000125 0.000120
Lipid Particles 0.023971 0.005513 0.000995 0.000753 0.008140 0.006585 0.010176 0.001067 0.015145 0.006072 0.001921 0.000922
Mitochondria 0.006861 0.008036 0.000507 0.000701 0.004189 0.008466 0.000486 0.000972 0.005372 0.008260 0.000505 0.000847
Mitotic Spindle 0.000641 0.005555 0.003052 0.001664 0.002226 0.007036 0.000087 0.001456 0.001525 0.006327 0.002753 0.001551
None 0.009894 0.002721 0.004884 0.002882 0.003312 0.003566 0.010873 0.003173 0.006225 0.003162 0.005488 0.003039
Nuclear Periphery 0.000955 0.000682 0.000954 0.000836 0.000521 0.000633 0.000886 0.000768 0.000714 0.000656 0.000947 0.000800
Nuclear Periphery Foci 0.000369 0.000277 0.000217 0.000159 0.000179 0.000147 0.001079 0.000131 0.000263 0.000209 0.000304 0.000144
Nucleolus 0.001505 0.001889 0.000567 0.000555 0.000963 0.001689 0.001650 0.000487 0.001203 0.001785 0.000676 0.000518
Nucleus 0.246644* 0.172759* 0.455107* 0.427952* 0.271521* 0.244208* 0.530600* 0.384007* 0.260512* 0.210023* 0.462719* 0.404237*
Peroxisomes 0.008210 0.012956 0.000439 0.000339 0.002735 0.006510 0.000915 0.000319 0.005157 0.009594 0.000487 0.000328
Vacuole 0.043361 0.070061 0.024778 0.026821 0.022025 0.047778 0.055087 0.030993 0.031467 0.058439 0.027834 0.029072
Vacuole Periphery 0.000909 0.002001 0.000293 0.000183 0.000447 0.001116 0.000242 0.000266 0.000651 0.001539 0.000288 0.000228

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.02 -1.23 -0.55 2.51 0.80 -2.20 -1.23 1.49 2.67 1.56 -3.93 -1.38 0.16 3.67 1.39
Bud Neck -4.38 10.78 8.77 12.82 -2.02 -9.94 9.08 6.14 13.90 -4.23 -10.31 12.46 10.60 18.96 -2.28
Bud Site -7.61 -4.29 -3.76 1.93 1.61 -4.75 3.04 -1.78 2.82 -4.84 -8.11 -3.83 -3.76 3.41 1.84
Cell Periphery 12.05 9.88 11.67 -1.03 1.84 -1.65 -5.39 1.16 3.20 6.41 9.65 6.98 10.44 2.29 2.05
Cytoplasm -3.47 -3.42 -9.15 -5.54 -4.48 4.79 10.92 -1.09 -5.83 -11.34 1.53 4.35 -6.69 -8.12 -8.95
Cytoplasmic Foci -0.50 33.04 33.75 38.01 1.02 1.02 8.58 28.24 27.33 6.63 -0.20 40.90 43.53 45.84 0.36
Eisosomes 8.35 9.53 9.64 19.71 2.93 -0.64 -3.22 15.05 15.30 10.77 8.05 10.85 11.49 22.54 5.98
Endoplasmic Reticulum 4.14 4.31 5.40 4.58 5.89 0.20 -2.21 0.14 -0.04 2.36 3.59 2.57 4.51 2.10 4.10
Endosome -4.83 10.88 11.14 15.35 0.88 -7.27 -1.20 6.32 11.17 4.08 -8.43 11.35 12.72 18.88 1.81
Golgi -0.67 6.83 6.82 8.36 -0.17 -2.25 0.51 4.14 3.94 0.92 -2.27 7.97 7.98 8.06 0.02
Lipid Particles 11.63 14.78 14.97 13.44 2.83 2.01 -0.36 10.39 12.84 2.24 10.81 14.94 17.88 18.30 2.44
Mitochondria -0.84 6.58 6.28 8.89 -0.92 -3.16 4.07 3.33 6.97 -1.00 -3.05 7.43 6.55 10.76 -1.39
Mitotic Spindle -4.13 -2.58 -1.64 3.27 1.44 -2.77 0.85 0.97 3.76 -0.19 -4.51 -1.22 0.19 4.97 1.49
None 4.36 3.16 4.29 -0.35 3.24 -0.45 -1.84 0.27 0.67 1.87 3.92 1.17 4.16 0.29 3.56
Nuclear Periphery 2.15 -1.54 0.67 -2.31 4.16 -2.49 -4.18 -7.33 -3.71 2.10 0.78 -6.67 -2.77 -3.89 5.70
Nuclear Periphery Foci 1.41 2.01 3.43 3.76 3.00 1.12 -2.91 1.61 0.38 3.00 1.61 -1.85 3.76 3.23 4.39
Nucleolus -0.60 2.75 2.74 6.38 0.04 -3.01 -1.70 3.88 5.91 2.43 -2.15 2.76 3.96 8.71 1.81
Nucleus 9.92 -21.71 -21.51 -33.44 3.31 3.86 -10.07 -15.45 -19.08 5.33 9.80 -24.23 -26.00 -36.51 7.20
Peroxisomes -3.06 8.03 8.11 10.76 0.26 -5.72 4.74 8.01 11.02 2.04 -5.52 10.01 10.49 14.62 0.86
Vacuole -8.75 3.74 5.34 14.26 1.17 -11.89 -4.58 -6.64 5.85 3.48 -14.56 -2.13 -0.14 14.39 2.04
Vacuole Periphery -4.89 5.09 7.88 8.99 1.10 -6.25 2.63 2.22 7.36 -0.06 -7.43 4.13 6.95 11.47 0.59
Standard name
Human Ortholog
Description Beta 5 subunit of the 20S proteasome; responsible for the chymotryptic activity of the proteasome
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Pre2

Pre2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pre2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available