Standard name
Human Ortholog
Description Putative regulator of the Fps1p glycerol channel; multiply phosphorylated by Hog1p under osmotic stress; contains a pleckstrin homology domain; forms homodimers and heterodimerizes with paralog Ask10p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud 0 0 0 0 0 0 0 0 0.06 0.06 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.05 0 0 0.12 0.14 0.15 0.12 0.07 0.11 0.14 0.15 0.14 0.12 0.09 0.13 0.14 0.13 0.19 0 0 0.07 0.06 0.07 0.1
Cytoplasm 0.97 0.97 0.97 0.96 0.93 0.84 0.77 0.76 0.65 0.66 0.64 0.63 0.98 1.0 0.98 0.94 0.92 0.95 0.9 0.85 0.71 0.8 0.78 0.73
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.09 0.09 0.22 0.24 0.31 0.46 0.47 0.47 0.51 0 0 0 0 0 0 0 0 0.1 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 4 7 11 12 7 7 9 6 6 0 0 1 0 0 0 1 3 10 3 4 13
Bud 0 1 6 8 10 18 19 30 26 35 24 26 2 2 7 0 0 0 0 0 0 0 3 0
Bud Neck 0 0 2 0 3 0 1 1 0 0 0 0 0 0 0 1 0 0 2 5 9 2 4 7
Bud Site 0 0 0 0 2 0 0 0 2 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 8 7 11 45 65 70 76 55 48 83 55 57 17 20 34 16 18 35 3 3 25 5 11 25
Cytoplasm 165 190 343 348 419 386 477 568 298 394 234 262 140 232 248 111 131 172 156 172 243 74 132 184
Endoplasmic Reticulum 2 2 2 2 0 2 4 2 0 8 2 4 2 0 0 1 9 5 1 1 5 1 0 6
Endosome 1 0 1 0 0 0 2 0 0 0 0 0 0 0 0 0 2 0 1 1 2 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 3 0 0 0 0 1 0
Mitochondria 0 7 9 33 40 100 149 230 210 283 173 210 1 0 6 2 1 4 3 9 33 2 3 8
Nucleus 0 0 0 1 0 1 1 7 3 3 3 0 0 1 0 0 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 2 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 1 0 0 1 1 0 0 6 2 2 1 0 0 0 0 0 0 1 1 0 2 0 0 0
Vac/Vac Membrane 0 0 0 1 1 0 1 5 1 2 1 2 0 0 1 3 6 6 0 1 2 1 4 0
Unique Cell Count 170 196 353 363 452 461 617 751 455 598 365 415 143 233 253 118 143 182 174 203 343 93 170 254
Labelled Cell Count 177 207 375 443 548 588 742 915 598 819 500 569 162 255 297 135 170 226 174 203 343 93 170 254


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 4.2 3.9 4.0 4.6 4.3 4.2 4.2 3.9 3.9 4.0 3.7 5.1 5.1 4.8 6.9 7.6 7.5 5.4 5.7 6.6
Std Deviation (1e-4) 0.7 0.6 0.7 1.5 1.7 1.2 1.1 1.2 1.2 1.0 1.2 0.9 0.9 0.7 0.9 1.3 2.3 1.9 1.3 1.3 1.1
Intensity Change (Log2) – – – 0.03 0.24 0.16 0.11 0.12 0.01 0.02 0.05 -0.06 0.39 0.41 0.29 0.83 0.97 0.94 0.48 0.55 0.76


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4977 1.2639 1.2153 0.7469 0.885 1.0346 1.4094 1.5869 1.3088 0.5209 1.0591 1.5007 1.6438 3.1333 2.2853 2.3694 2.5115 2.4385
Actin 0.0638 0.0021 0.0054 0.0011 0.061 0.0051 0.0646 0.0012 0.0242 0.0043 0.0127 0.023 0.0008 0.0004 0.0101 0.0201 0.0208 0.002
Bud 0.0007 0.0002 0.0043 0.0003 0.0007 0.0006 0.0083 0.0078 0.0098 0.0106 0.0065 0.0051 0.0001 0.0002 0.0009 0.0081 0.0015 0.0002
Bud Neck 0.0018 0.0004 0.0003 0.0002 0.0009 0.0069 0.0018 0.0003 0.0117 0.0001 0.0011 0.0019 0.0001 0.0001 0.0003 0.0093 0.0028 0.0006
Bud Periphery 0.001 0.0003 0.0017 0.0003 0.0012 0.0006 0.0145 0.0069 0.008 0.0547 0.0178 0.007 0.0001 0.0004 0.0019 0.0197 0.0044 0.0014
Bud Site 0.0036 0.0098 0.0076 0.0002 0.0018 0.0003 0.0104 0.0239 0.0302 0.0005 0.0029 0.0019 0.0004 0.0017 0.0022 0.0044 0.0007 0
Cell Periphery 0.0017 0.0013 0.0007 0.0003 0.0003 0.0003 0.0007 0.0011 0.0014 0.0001 0.0005 0.0004 0.0006 0.0015 0.0007 0.0007 0.0004 0.0004
Cytoplasm 0.3426 0.5407 0.572 0.5916 0.4589 0.6182 0.244 0.4208 0.3951 0.4696 0.2496 0.5509 0.3378 0.3609 0.4258 0.3953 0.4079 0.4973
Cytoplasmic Foci 0.0465 0.0043 0.0174 0.0083 0.0107 0.017 0.0462 0.0036 0.021 0.0036 0.0112 0.0164 0.0036 0.0022 0.0061 0.0348 0.0373 0.0036
Eisosomes 0.0004 0.0002 0.0002 0 0.0006 0.0001 0.0007 0.0001 0.0006 0.0001 0.0001 0.0001 0.0002 0.0002 0.0004 0.0005 0.0003 0.0002
Endoplasmic Reticulum 0.008 0.0034 0.0042 0.0072 0.0029 0.0035 0.009 0.0019 0.0036 0.001 0.0072 0.0019 0.003 0.001 0.0028 0.0042 0.0063 0.0014
Endosome 0.0431 0.0013 0.006 0.0119 0.0084 0.0027 0.042 0.0007 0.0128 0.0012 0.0569 0.0075 0.0005 0.0003 0.0023 0.0179 0.0168 0.0009
Golgi 0.0094 0.0002 0.0026 0.0008 0.0079 0.0011 0.0144 0.0002 0.0033 0.0003 0.0077 0.0068 0.0002 0.0001 0.0008 0.0107 0.0138 0.0001
Lipid Particles 0.0137 0.0006 0.0028 0.0011 0.0139 0.0005 0.0169 0.0003 0.0055 0.0001 0.0136 0.0075 0.0004 0.0001 0.0006 0.0113 0.0093 0.0001
Mitochondria 0.0039 0.0001 0.0047 0.0005 0.0041 0.0008 0.0226 0.0004 0.0105 0.0033 0.0033 0.0022 0.0001 0.0001 0.0026 0.0155 0.0093 0.0002
None 0.3901 0.432 0.3617 0.3697 0.4014 0.3373 0.4291 0.5274 0.448 0.4475 0.5877 0.3583 0.6507 0.6294 0.5357 0.4162 0.4498 0.4893
Nuclear Periphery 0.0157 0.0004 0.0018 0.0018 0.0038 0.0018 0.0266 0.0003 0.0012 0.0004 0.0021 0.0009 0.0002 0.0001 0.0005 0.0083 0.0058 0.0002
Nucleolus 0.0012 0.0001 0.0003 0.0001 0.0006 0.0001 0.0014 0.0002 0.0007 0.0001 0.0007 0.0001 0.0001 0.0001 0.0001 0.0011 0.0002 0.0001
Nucleus 0.0042 0.0006 0.0009 0.0008 0.0009 0.0006 0.0066 0.0009 0.0017 0.0005 0.001 0.0009 0.0003 0.0002 0.0006 0.0023 0.0007 0.0003
Peroxisomes 0.0078 0.0001 0.0025 0.0001 0.0085 0.0009 0.0229 0.0002 0.004 0.0007 0.0041 0.005 0.0001 0.0001 0.0041 0.01 0.0072 0.0001
Punctate Nuclear 0.021 0.0003 0.0015 0.0006 0.0106 0.0008 0.0108 0.0006 0.0023 0.0002 0.0009 0.001 0.0002 0.0001 0.0004 0.006 0.0012 0.0007
Vacuole 0.0174 0.0014 0.0011 0.0028 0.0007 0.0007 0.0033 0.0012 0.0039 0.0011 0.0101 0.001 0.0005 0.0009 0.0009 0.0025 0.0018 0.0007
Vacuole Periphery 0.0024 0.0001 0.0003 0.0004 0.0004 0.0002 0.0031 0.0001 0.0007 0.0002 0.0021 0.0002 0 0 0.0001 0.0012 0.0017 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.965 17.8955 17.7807 24.8895 18.2566 21.3641 21.0843 23.9072 24.5265 25.3627
Translational Efficiency 0.2281 0.2136 0.2648 0.2247 0.2644 0.2268 0.2168 0.2019 0.3098 0.2534

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1926 1005 148 966 1449 1044 369 226 3375 2049 517 1192

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 699.30 702.68 1163.31 832.67 612.92 771.64 1017.38 882.95 662.21 737.82 1059.15 842.20
Standard Deviation 76.38 90.70 139.79 113.49 77.03 100.59 104.35 95.38 87.78 101.87 133.10 112.03
Intensity Change Log 2 — 0.006956 0.734252 0.251833 — 0.332229 0.731088 0.526633 — 0.168040 0.732775 0.386720

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000478 0.001072 0.001574 0.001437 0.000253 0.000909 0.000555 0.001052 0.000382 0.000989 0.000847 0.001364
Bud Neck 0.020780 0.035075 0.003506 0.006803 0.011787 0.036282 0.002223 0.002209 0.016919 0.035690 0.002590 0.005932
Bud Site 0.009717 0.022395 0.020248 0.031836 0.006377 0.026066 0.007666 0.013560 0.008283 0.024265 0.011268 0.028371
Cell Periphery 0.000705 0.000290 0.000676 0.000431 0.000209 0.000326 0.000388 0.000261 0.000492 0.000308 0.000470 0.000399
Cytoplasm 0.577666* 0.440807* 0.395217* 0.504092* 0.516410* 0.499158* 0.476041* 0.479432* 0.551367* 0.470538* 0.452903* 0.499416*
Cytoplasmic Foci 0.182467* 0.281306* 0.073645 0.036901 0.247658* 0.230339* 0.045730 0.031948 0.210456* 0.255337* 0.053721 0.035962
Eisosomes 0.001514 0.000599 0.000136 0.000168 0.000377 0.000649 0.000130 0.000123 0.001026 0.000624 0.000132 0.000160
Endoplasmic Reticulum 0.001409 0.001069 0.008680 0.002569 0.001793 0.000469 0.004329 0.002085 0.001574 0.000763 0.005575 0.002477
Endosome 0.008793 0.041345 0.013164 0.006625 0.018719 0.026497 0.007353 0.001717 0.013054 0.033780 0.009017 0.005694
Golgi 0.004642 0.008177 0.000387 0.003836 0.003840 0.009806 0.000126 0.000063 0.004298 0.009007 0.000201 0.003121
Lipid Particles 0.006788 0.008799 0.006295 0.001487 0.014710 0.004720 0.003434 0.001217 0.010189 0.006721 0.004253 0.001436
Mitochondria 0.002218 0.005907 0.000122 0.001706 0.002674 0.005088 0.000125 0.000084 0.002413 0.005490 0.000124 0.001398
Mitotic Spindle 0.000949 0.000758 0.002434 0.021837 0.000191 0.003587 0.000444 0.000405 0.000623 0.002199 0.001013 0.017774
None 0.006311 0.001337 0.000652 0.005130 0.008401 0.001426 0.001643 0.001879 0.007208 0.001382 0.001359 0.004514
Nuclear Periphery 0.000126 0.000338 0.001021 0.000660 0.000474 0.000106 0.001006 0.000531 0.000275 0.000220 0.001010 0.000636
Nuclear Periphery Foci 0.000652 0.000467 0.024142 0.001950 0.001121 0.000265 0.006647 0.001578 0.000854 0.000364 0.011656 0.001879
Nucleolus 0.000860 0.000985 0.000168 0.000247 0.002099 0.000585 0.000218 0.000074 0.001392 0.000781 0.000203 0.000214
Nucleus 0.110191 0.039616 0.094286 0.164722* 0.085707 0.043996 0.160015* 0.127914 0.099679 0.041848 0.141199* 0.157743*
Peroxisomes 0.003314 0.013281 0.000578 0.002982 0.003653 0.008966 0.000331 0.000230 0.003460 0.011083 0.000402 0.002461
Vacuole 0.059839 0.094389 0.352773* 0.203991* 0.072304 0.098450 0.281448* 0.333587* 0.065191 0.096458 0.301866* 0.228562*
Vacuole Periphery 0.000581 0.001988 0.000296 0.000592 0.001245 0.002310 0.000148 0.000051 0.000866 0.002152 0.000190 0.000489

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.46 -7.57 -1.49 0.22 2.02 -2.81 -3.30 -1.70 0.43 -0.73 -2.63 -5.85 -2.03 0.21 -0.23
Bud Neck -5.48 14.02 11.50 11.95 -1.64 -9.84 9.42 8.79 14.42 -0.74 -10.40 18.25 12.10 17.39 -3.24
Bud Site -6.60 -5.03 -3.90 1.23 0.61 -7.99 -0.60 -1.53 3.59 -1.32 -10.15 -2.53 -4.67 2.84 -3.12
Cell Periphery 9.69 1.02 6.37 -0.32 4.20 -2.88 -3.44 0.09 2.50 2.87 6.02 2.09 4.16 0.36 2.47
Cytoplasm 14.37 11.49 16.98 3.06 -2.47 1.60 7.27 7.87 6.81 1.85 11.51 13.64 17.04 7.40 -0.39
Cytoplasmic Foci -13.45 17.84 40.37 37.86 5.78 2.20 37.50 38.51 30.01 1.97 -8.35 42.62 55.58 48.26 5.49
Eisosomes 6.50 15.13 13.99 3.94 -0.29 -3.20 4.82 4.92 7.55 0.59 4.72 15.61 13.77 6.86 -0.29
Endoplasmic Reticulum 0.69 -6.07 -4.10 -6.71 4.67 5.75 -6.71 -2.17 -7.55 4.81 2.78 -8.66 -5.19 -11.25 5.97
Endosome -11.59 -2.54 5.95 13.48 6.94 -3.99 11.29 17.53 14.43 7.96 -12.17 6.95 13.53 18.25 6.64
Golgi -4.68 14.62 4.14 6.77 -1.62 -5.43 13.60 13.77 9.15 4.87 -6.99 20.15 5.08 9.07 -1.84
Lipid Particles -2.27 1.42 13.73 8.53 8.23 9.99 12.25 14.43 7.37 5.76 5.37 12.33 18.88 10.85 9.09
Mitochondria -3.00 5.33 2.91 4.25 -2.83 -2.29 6.77 6.88 5.09 3.17 -3.80 8.27 4.93 6.08 -2.77
Mitotic Spindle 0.35 -2.08 -4.77 -4.93 -3.64 -2.91 -1.78 -2.45 1.88 -1.46 -2.29 -1.57 -5.01 -4.09 -4.66
None 7.98 9.81 3.38 -3.19 -4.60 5.44 5.69 5.58 0.00 -0.35 8.84 9.61 5.16 -3.09 -3.79
Nuclear Periphery -1.84 -9.42 -18.98 -5.91 0.69 6.44 -8.92 -3.82 -11.24 5.22 0.86 -15.27 -15.52 -11.39 2.77
Nuclear Periphery Foci 2.40 -13.51 -10.28 -11.53 12.58 8.51 -8.51 -3.85 -8.38 6.78 8.07 -14.19 -9.93 -14.60 12.32
Nucleolus -0.72 7.62 6.80 3.08 -1.62 5.10 6.70 7.05 5.68 4.61 2.91 9.08 8.88 4.35 -0.49
Nucleus 21.52 2.01 -11.12 -21.22 -8.77 11.97 -9.53 -3.50 -7.71 4.05 24.29 -7.13 -12.62 -22.55 -2.58
Peroxisomes -7.55 11.49 4.28 8.81 -1.88 -5.60 9.31 9.41 9.93 1.13 -9.45 14.98 6.08 11.81 -2.26
Vacuole -8.64 -17.47 -27.31 -22.26 4.11 -5.75 -19.27 -19.04 -17.56 -4.58 -10.48 -25.71 -32.07 -27.55 1.18
Vacuole Periphery -7.20 4.72 2.60 8.15 -0.10 -1.75 12.62 13.56 3.76 7.45 -3.96 13.17 6.64 5.80 -0.87
Standard name
Human Ortholog
Description Putative regulator of the Fps1p glycerol channel; multiply phosphorylated by Hog1p under osmotic stress; contains a pleckstrin homology domain; forms homodimers and heterodimerizes with paralog Ask10p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
Localization
Cell Percentages cytoplasm (66%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Rgc1

Rgc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rgc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available