Standard name
Human Ortholog
Description B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome; CLB2 has a paralog, CLB1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.14 0.06 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.68 0.57 0.68 0.65 0.72 0.75 0.6 0.68 0.66 0.65 0.57 0.63 0.64 0.63 0.58 0.66 0.61 0.6 0.53 0.33 0.36 0.77 0.93 0.84
Endoplasmic Reticulum 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.27 0 0.1 0.15 0.19 0.3 0.36 0.31 0.42 0.44 0.55 0.5 0 0 0 0.06 0 0 0 0 0.1 0 0 0
Nucleus 0.14 0.32 0.24 0.25 0.1 0.11 0.18 0.18 0.15 0.12 0.16 0.1 0.33 0.27 0.26 0.23 0.21 0.21 0.2 0.33 0.26 0.05 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0.06 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0
Vac/Vac Membrane 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0.08 0.14 0.12 0 0.07 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 2 0 0 2
Bud 0 1 1 1 0 0 3 3 2 5 1 2 0 0 0 1 0 0 5 39 8 3 0 1
Bud Neck 0 1 1 0 0 0 2 4 2 0 1 1 0 0 0 0 0 0 0 1 0 0 0 2
Bud Site 0 0 0 0 0 0 1 3 2 1 2 2 0 2 0 0 0 0 – – – – – –
Cell Periphery 0 2 0 1 2 1 2 0 5 0 1 1 0 0 0 0 0 5 0 0 0 0 0 0
Cytoplasm 15 159 101 46 90 106 116 173 123 122 76 92 29 83 67 125 156 94 32 97 55 56 122 103
Endoplasmic Reticulum 1 3 0 1 1 0 0 0 2 0 0 1 0 0 0 7 24 12 0 0 0 0 1 1
Endosome 0 4 1 0 2 0 0 0 0 0 0 0 0 1 4 6 8 4 2 2 2 0 0 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 3 1 0 0 0
Mitochondria 6 7 15 11 24 43 69 79 77 83 74 73 0 2 4 11 7 7 2 10 15 2 2 2
Nucleus 3 88 35 18 13 16 35 46 27 22 22 15 15 35 30 44 54 33 12 95 39 3 0 0
Nuclear Periphery 0 2 2 2 0 1 1 1 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 4 1 1 2 3 1 5 2 7 0 4 2 0 2 0 0 0 0
Peroxisomes 0 3 0 0 0 0 0 0 0 0 0 0 0 2 1 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 1 2 0 3 0 0 2 0 2 1 1 1 1 2 13 10 1 0 0
Vac/Vac Membrane 1 23 3 0 3 4 5 3 2 0 0 0 1 8 10 16 35 18 0 19 7 2 0 4
Unique Cell Count 22 278 148 71 125 141 192 256 185 187 134 145 45 132 116 189 256 156 61 293 153 73 132 124
Labelled Cell Count 26 293 159 81 136 172 240 313 246 235 180 190 50 137 125 212 291 177 61 293 153 73 132 124


Ambiguous, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 4.6 3.9 4.3 3.9 3.0 2.6 3.3 3.0 2.5 2.6 2.6 5.0 4.7 5.0 5.5 6.8 7.0 3.5 3.8 4.5
Std Deviation (1e-4) 1.0 1.5 2.0 2.9 2.5 1.7 1.2 2.0 1.9 1.3 1.2 1.9 1.5 1.5 1.9 1.8 2.6 2.7 0.9 1.5 1.2
Intensity Change (Log2) – – – 0.12 -0.03 -0.38 -0.58 -0.25 -0.41 -0.64 -0.59 -0.58 0.36 0.25 0.35 0.49 0.79 0.83 -0.15 -0.05 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.5 3.9 3.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.0782 2.8308 3.6992 5.4379 6.2353 2.4901 2.6151 1.2706 2.0948 3.4238 3.8824 1.6598 2.2418 2.1748 2.1738 4.8904 4.8142 1.8373
Actin 0.0602 0.0008 0.0196 0.0304 0.0081 0.0031 0.0568 0.0025 0.0105 0.0283 0.0205 0.0037 0.0367 0.005 0.0071 0.0281 0.0068 0.0006
Bud 0.0073 0.0178 0.0021 0.0007 0.0013 0.0023 0.0124 0.0022 0.0036 0.0051 0.0009 0.0011 0.0021 0.0093 0.0053 0.0004 0.0008 0.0017
Bud Neck 0.0068 0.0013 0.0064 0.0009 0.0037 0.0028 0.013 0.0012 0.0012 0.0059 0.0029 0.0028 0.024 0.0041 0.0008 0.0022 0.0011 0.0032
Bud Periphery 0.0099 0.0156 0.0073 0.0017 0.0022 0.0024 0.0184 0.0026 0.0042 0.004 0.0019 0.0025 0.0043 0.005 0.0041 0.0007 0.0004 0.0035
Bud Site 0.0217 0.0443 0.0114 0.0017 0.0027 0.0017 0.0264 0.0209 0.0094 0.0053 0.0016 0.0003 0.0234 0.0245 0.0072 0.0012 0.0004 0.0004
Cell Periphery 0.0014 0.0014 0.0005 0.0001 0.0002 0.0001 0.0014 0.0005 0.0005 0.0004 0.0003 0.0001 0.0014 0.0011 0.0003 0.0001 0.0001 0.0003
Cytoplasm 0.0491 0.0611 0.0452 0.0025 0.0091 0.0515 0.0355 0.0303 0.038 0.0171 0.009 0.0475 0.0427 0.0445 0.0635 0.0065 0.0128 0.0583
Cytoplasmic Foci 0.0303 0.004 0.0057 0.0061 0.0035 0.01 0.0435 0.0135 0.0134 0.0163 0.0082 0.0076 0.0096 0.0044 0.0073 0.0093 0.0048 0.0059
Eisosomes 0.0007 0.0002 0.0004 0.0001 0.0003 0.0001 0.0008 0.0003 0.0005 0.0001 0.0002 0.0001 0.0008 0.0005 0.0002 0.0003 0.0001 0.0001
Endoplasmic Reticulum 0.0117 0.0016 0.0024 0.0002 0.0074 0.0013 0.0067 0.0009 0.0019 0.0041 0.0091 0.0016 0.0125 0.0024 0.004 0.0007 0.0006 0.001
Endosome 0.0275 0.001 0.0036 0.0056 0.0158 0.007 0.0543 0.0005 0.0136 0.0252 0.0284 0.0077 0.0156 0.0013 0.0074 0.003 0.0051 0.001
Golgi 0.0097 0.0001 0.0009 0.0019 0.005 0.0012 0.0126 0.0003 0.0107 0.0204 0.009 0.0096 0.0048 0.0004 0.0012 0.0026 0.0022 0.0001
Lipid Particles 0.0134 0.0004 0.0046 0.0097 0.0129 0.0055 0.0345 0.0007 0.0065 0.0203 0.0266 0.0014 0.0088 0.0009 0.0074 0.0024 0.0065 0.0006
Mitochondria 0.0091 0.0023 0.0013 0.0006 0.0151 0.001 0.0123 0.0007 0.0105 0.0042 0.0146 0.0054 0.0169 0.0011 0.0018 0.0159 0.0011 0.0017
None 0.481 0.5333 0.3088 0.0046 0.0147 0.701 0.4074 0.7154 0.3876 0.0107 0.0334 0.6392 0.6501 0.5635 0.4018 0.0162 0.0472 0.6654
Nuclear Periphery 0.0247 0.0052 0.0163 0.0032 0.0348 0.0044 0.0284 0.0044 0.0091 0.008 0.0417 0.0061 0.0437 0.0071 0.0201 0.0039 0.0291 0.0034
Nucleolus 0.0116 0.0127 0.0064 0.0121 0.0358 0.0082 0.0147 0.0124 0.0065 0.0056 0.0291 0.0063 0.004 0.0098 0.0054 0.0159 0.0449 0.0137
Nucleus 0.1666 0.2792 0.5239 0.9054 0.7911 0.1706 0.1377 0.1735 0.4312 0.8045 0.7226 0.2328 0.0642 0.2841 0.4324 0.8651 0.7858 0.2202
Peroxisomes 0.0087 0.0004 0.0072 0.0041 0.0071 0.0078 0.0368 0.0018 0.0117 0.0079 0.014 0.0021 0.0038 0.0002 0.0012 0.0023 0.0016 0.0032
Punctate Nuclear 0.0272 0.0123 0.0213 0.0077 0.0244 0.0159 0.022 0.0137 0.0261 0.0026 0.018 0.0198 0.0222 0.0268 0.019 0.0221 0.0474 0.0128
Vacuole 0.016 0.0047 0.0042 0.0005 0.0028 0.0014 0.0205 0.0014 0.0026 0.0032 0.0058 0.0016 0.005 0.0035 0.0016 0.0006 0.0006 0.0023
Vacuole Periphery 0.0053 0.0006 0.0007 0.0001 0.0021 0.0005 0.0042 0.0003 0.0008 0.0007 0.0023 0.0006 0.0033 0.0004 0.0007 0.0004 0.0007 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.7183 67.4959 66.4493 57.3564 15.0234 10.4601 78.0426 92.3497 55.4664 22.2965
Translational Efficiency 1.3031 1.2144 1.4306 1.1913 1.2543 0.7089 1.1159 1.1486 1.2301 1.0421

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
826 1364 369 476 572 1745 158 150 1398 3109 527 626

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 611.95 673.86 833.05 999.62 728.61 711.38 950.07 1015.74 659.68 694.92 868.13 1003.48
Standard Deviation 83.12 138.08 98.49 198.79 85.20 104.58 106.13 212.94 101.70 121.86 114.21 202.39
Intensity Change Log 2 — 0.139035 0.444989 0.707966 — -0.034526 0.382887 0.479312 — 0.047300 0.411568 0.588202

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000156 0.000360 0.000283 0.003088 0.000159 0.000422 0.000530 0.001055 0.000157 0.000395 0.000357 0.002601
Bud Neck 0.008443 0.023343 0.005431 0.032213 0.002338 0.036458 0.003377 0.016378 0.005945 0.030704 0.004815 0.028419
Bud Site 0.003232 0.011136 0.001383 0.018513 0.001328 0.015526 0.002042 0.009239 0.002453 0.013600 0.001580 0.016291
Cell Periphery 0.000648 0.000427 0.000131 0.000207 0.000316 0.000378 0.000153 0.000279 0.000512 0.000399 0.000137 0.000224
Cytoplasm 0.292384* 0.342673* 0.106511* 0.068865 0.344592* 0.336292* 0.144002* 0.093298 0.313745* 0.339092* 0.117751* 0.074719
Cytoplasmic Foci 0.042197 0.083520 0.007782 0.001792 0.086307 0.120257 0.004437 0.007143 0.060245 0.104139 0.006779 0.003074
Eisosomes 0.000069 0.000115 0.000048 0.000039 0.000079 0.000118 0.000061 0.000047 0.000073 0.000117 0.000052 0.000041
Endoplasmic Reticulum 0.000831 0.001733 0.003304 0.008514 0.001288 0.001764 0.006592 0.005702 0.001018 0.001751 0.004290 0.007840
Endosome 0.001167 0.011831 0.001698 0.002698 0.002796 0.016078 0.002017 0.014298 0.001833 0.014215 0.001794 0.005478
Golgi 0.000316 0.001427 0.000074 0.000722 0.000234 0.004728 0.000084 0.028848 0.000283 0.003280 0.000077 0.007461
Lipid Particles 0.002230 0.004657 0.001543 0.000739 0.003484 0.006502 0.001559 0.000999 0.002743 0.005693 0.001548 0.000801
Mitochondria 0.002233 0.005719 0.000791 0.004775 0.000562 0.017423 0.000722 0.003022 0.001549 0.012288 0.000770 0.004355
Mitotic Spindle 0.001160 0.006988 0.002483 0.065582 0.000373 0.021189 0.005897 0.029505 0.000838 0.014959 0.003507 0.056937
None 0.047995 0.062097 0.006257 0.011773 0.017319 0.019227 0.005320 0.013986 0.035444 0.038036 0.005976 0.012303
Nuclear Periphery 0.000476 0.000678 0.001639 0.003725 0.000495 0.001039 0.002078 0.001910 0.000484 0.000881 0.001771 0.003290
Nuclear Periphery Foci 0.000580 0.001616 0.000805 0.006052 0.000760 0.000769 0.001182 0.001272 0.000654 0.001140 0.000918 0.004907
Nucleolus 0.021560 0.017526 0.006974 0.006248 0.010084 0.014761 0.002569 0.002825 0.016864 0.015974 0.005653 0.005428
Nucleus 0.567710* 0.384720* 0.834575* 0.733232* 0.512984* 0.332941* 0.763370* 0.686578* 0.545319* 0.355657* 0.813227* 0.722053*
Peroxisomes 0.001524 0.006492 0.000283 0.001649 0.000908 0.011769 0.000208 0.000280 0.001272 0.009454 0.000261 0.001321
Vacuole 0.004744 0.031547 0.017811 0.028769 0.013336 0.039982 0.053609 0.074637 0.008260 0.036281 0.028544 0.039760
Vacuole Periphery 0.000345 0.001397 0.000195 0.000805 0.000258 0.002373 0.000191 0.008699 0.000309 0.001945 0.000194 0.002696

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.56 -3.85 -7.21 -6.50 -6.05 -3.70 -5.29 -6.80 -5.68 -4.03 -5.10 -6.00 -8.62 -7.61 -6.80
Bud Neck -6.18 -0.45 -7.11 -3.81 -6.86 -16.27 -3.31 -5.05 0.76 -4.59 -14.26 -1.34 -9.03 -2.04 -8.24
Bud Site -6.15 1.20 -5.49 -2.51 -5.93 -12.45 -3.13 -3.81 -0.44 -3.45 -12.67 -0.15 -6.83 -1.81 -6.85
Cell Periphery 1.91 4.46 3.74 5.29 -4.36 -1.08 2.84 0.97 2.81 -2.56 1.61 5.14 3.97 6.62 -4.94
Cytoplasm -3.39 14.60 18.96 25.88 2.94 0.66 9.96 13.61 15.67 2.45 -2.27 18.27 24.58 32.92 4.02
Cytoplasmic Foci -7.97 10.18 12.81 20.36 4.02 -4.48 13.58 13.81 26.28 1.13 -9.95 16.47 18.72 33.86 4.34
Eisosomes -1.78 2.10 0.30 1.85 -1.71 -5.04 -1.61 -0.61 3.11 0.83 -3.59 1.02 0.37 3.67 -0.59
Endoplasmic Reticulum -5.75 -9.05 -10.64 -9.47 -5.69 -2.75 -7.79 -7.89 -7.45 0.02 -6.29 -11.36 -12.74 -11.61 -4.47
Endosome -9.38 -2.60 -2.04 8.67 0.49 -12.03 0.47 -1.26 3.20 -1.34 -15.72 -1.18 -1.44 10.83 -0.98
Golgi -5.88 1.83 -0.56 5.66 -2.87 -6.89 3.37 -1.24 -0.52 -1.26 -7.92 2.60 -1.29 0.69 -1.42
Lipid Particles -4.47 1.88 3.93 8.07 2.72 -3.55 2.98 3.64 9.79 1.23 -6.36 3.62 5.67 13.78 3.17
Mitochondria -2.65 1.26 -1.44 2.01 -6.09 -10.10 -0.83 -5.67 8.57 -5.31 -9.19 1.12 -2.97 8.22 -7.41
Mitotic Spindle -4.13 -2.74 -7.96 -7.16 -7.07 -7.55 -1.31 -4.26 -2.87 -3.58 -8.78 -2.84 -9.02 -7.06 -7.89
None -2.00 9.92 9.09 10.85 -2.61 -0.52 6.86 3.73 5.25 -3.96 -0.39 11.17 9.55 11.64 -3.85
Nuclear Periphery -3.50 -8.81 -8.61 -8.19 -5.38 -6.20 -10.04 -7.91 -6.44 -2.75 -6.96 -12.58 -10.46 -9.28 -5.85
Nuclear Periphery Foci -1.92 -1.06 -3.06 -2.07 -1.03 1.01 0.30 -0.91 -2.30 -1.57 0.26 -0.65 -2.54 -3.27 -1.50
Nucleolus 1.28 1.90 3.17 2.42 0.45 -1.06 2.94 1.49 3.34 -1.62 0.74 2.36 2.98 2.99 -0.08
Nucleus 11.83 -14.55 -6.04 -16.52 6.91 10.42 -5.37 -2.90 -9.01 1.60 16.68 -15.14 -7.35 -20.67 6.05
Peroxisomes -5.00 2.17 1.25 6.93 -2.15 -10.68 3.10 1.91 11.45 -1.51 -10.86 2.97 1.59 13.00 -2.50
Vacuole -12.36 -5.65 -8.45 -4.36 -4.70 -10.63 -7.42 -5.82 -4.42 0.74 -17.25 -8.42 -9.91 -5.84 -1.85
Vacuole Periphery -5.88 1.30 -0.27 5.85 -1.81 -10.77 2.18 -1.21 -0.12 -1.25 -11.75 1.70 -1.27 2.11 -1.48
Standard name
Human Ortholog
Description B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome; CLB2 has a paralog, CLB1, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (51%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Clb2

Clb2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Clb2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available