ORF
Human Ortholog
Description Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.06 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.97 – 0.96 0.98 0.98 0.94 0.89 0.79 0.85 0.66 0.75 1.0 1.0 1.0 0.97 0.96 0.99 0.8 0.89 0.86 0.87 0.86 0.88
Endoplasmic Reticulum 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – 0 0 0.07 0.15 0.14 0.41 0.29 0.5 0.34 0 0 0 0 0 0 0.09 0.05 0 0 0 0
Nucleus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 1
Bud 0 0 – 1 0 0 0 0 3 7 4 6 0 0 0 0 1 0 0 0 0 3 6 13
Bud Neck 3 0 – 2 9 4 1 6 2 4 1 2 1 1 0 0 0 0 0 0 0 0 0 2
Bud Site 1 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 2 – 0 3 4 0 0 0 0 0 1 3 0 0 7 4 5 2 0 1 1 3 3
Cytoplasm 268 31 – 115 294 248 325 315 190 311 131 195 225 296 263 250 128 160 233 62 42 126 211 300
Endoplasmic Reticulum 0 0 – 0 1 1 0 1 0 0 1 0 0 0 0 15 7 6 2 1 0 1 0 1
Endosome 0 0 – 0 2 0 0 0 0 0 0 0 0 1 0 0 2 0 1 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 2
Mitochondria 9 1 – 3 2 17 51 51 99 107 100 89 0 0 0 3 0 1 27 3 2 3 7 3
Nucleus 1 0 – 3 3 1 2 11 2 5 2 6 1 0 0 0 0 0 1 0 0 0 0 1
Nuclear Periphery 2 0 – 1 0 0 0 0 1 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 – 0 0 1 0 9 1 3 1 3 0 0 0 0 0 0 3 0 1 2 4 4
Vac/Vac Membrane 2 0 – 1 1 1 1 1 4 10 3 11 0 0 1 9 4 5 5 1 1 0 2 3
Unique Cell Count 279 32 120 301 254 347 353 242 366 200 260 225 297 264 257 133 162 291 70 50 146 245 343
Labelled Cell Count 290 34 126 315 277 380 394 303 448 245 313 230 298 264 284 147 177 291 70 50 146 245 343


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 4.5 – 4.2 4.7 4.0 3.8 4.2 3.5 4.0 3.7 4.1 5.0 5.2 5.4 7.0 7.5 7.4 5.9 5.9 6.6
Std Deviation (1e-4) 0.7 0.8 – 1.1 1.4 2.0 1.8 1.5 1.6 1.8 1.8 1.6 4.0 1.4 2.3 1.7 1.9 1.7 5.8 5.8 5.8
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4588 1.8533 1.0465 1.2708 1.4616 1.0727 -0.0653 0.1594 -0.0469 0.35 -0.9273 0.1558 -1.4174 -1.0012 -1.3159 -0.4331 -1.2138 -1.5619
Actin 0.0213 0.001 0.0046 0.04 0.0379 0.0067 0.0411 0.0089 0.0112 0.0448 0.0343 0.0032 0.0218 0.0017 0.0034 0.0317 0.0135 0.0436
Bud 0.0006 0.001 0.0016 0.0111 0.0008 0.0014 0.0027 0.0026 0.0103 0.0018 0.0004 0.0046 0.001 0.0025 0.0003 0.0017 0.0002 0.0006
Bud Neck 0.0013 0.0003 0.0005 0.02 0.0004 0.0006 0.0019 0.0002 0.0022 0.0028 0.0002 0.0005 0.004 0.0002 0.0002 0.0008 0.0006 0.0005
Bud Periphery 0.0014 0.0007 0.0045 0.0069 0.0011 0.0035 0.0026 0.0054 0.0062 0.0053 0.0006 0.0032 0.0012 0.0012 0.0002 0.0019 0.0002 0.001
Bud Site 0.0134 0.0056 0.0043 0.004 0.0009 0.0005 0.0074 0.0185 0.0079 0.0041 0.0015 0.0002 0.0223 0.0216 0.0011 0.013 0.0007 0.0002
Cell Periphery 0.0006 0.0001 0.0003 0.0002 0.0001 0.0001 0.0003 0.0003 0.0002 0.0061 0.0001 0.0001 0.0014 0.0002 0.0001 0.0003 0 0.0001
Cytoplasm 0.1836 0.2449 0.1997 0.227 0.3161 0.2429 0.0971 0.2066 0.1271 0.047 0.1227 0.2407 0.1535 0.2738 0.1483 0.3465 0.1035 0.3317
Cytoplasmic Foci 0.0406 0.0204 0.0155 0.0229 0.013 0.0053 0.0264 0.0089 0.0243 0.0244 0.041 0.0167 0.0281 0.0054 0.0152 0.0202 0.0536 0.0154
Eisosomes 0.0019 0.0001 0.0003 0.0002 0.0004 0.0001 0.0006 0.0001 0.0002 0.0121 0.0003 0.0001 0.0003 0.0001 0.0003 0.0002 0.0007 0.0002
Endoplasmic Reticulum 0.0056 0.001 0.0025 0.0016 0.0037 0.0011 0.0032 0.0008 0.0007 0.0061 0.0006 0.0009 0.0023 0.0023 0.0013 0.0026 0.0006 0.0032
Endosome 0.0323 0.0017 0.0171 0.0308 0.0264 0.0053 0.02 0.0053 0.0065 0.0191 0.0073 0.0052 0.0081 0.0012 0.0173 0.0533 0.0088 0.0146
Golgi 0.0094 0.0004 0.0062 0.0118 0.0112 0.0046 0.0077 0.001 0.0025 0.009 0.0065 0.0022 0.0054 0.0003 0.0121 0.0269 0.0222 0.0227
Lipid Particles 0.0146 0.0002 0.0052 0.0046 0.0123 0.0051 0.0201 0.0015 0.0044 0.0173 0.0331 0.0036 0.0257 0.0003 0.003 0.0015 0.0151 0.0067
Mitochondria 0.0271 0.0002 0.0104 0.0018 0.0052 0.0138 0.0112 0.0017 0.007 0.0225 0.0082 0.0012 0.002 0.0002 0.0026 0.0094 0.0053 0.0271
None 0.5922 0.7165 0.703 0.6061 0.5285 0.6962 0.7163 0.7306 0.7694 0.7465 0.7023 0.6982 0.6709 0.6673 0.7726 0.4801 0.6864 0.5226
Nuclear Periphery 0.0045 0.0004 0.0023 0.0015 0.0231 0.0016 0.0047 0.0005 0.0007 0.0035 0.0008 0.0047 0.0016 0.0007 0.0006 0.0013 0.0003 0.0012
Nucleolus 0.0039 0.0002 0.0008 0.0002 0.0003 0.0007 0.0011 0.0003 0.0004 0.0012 0.0008 0.0005 0.0005 0.0002 0.0001 0.0002 0.0001 0.0004
Nucleus 0.0029 0.0014 0.0017 0.0027 0.0082 0.0042 0.0037 0.0015 0.0019 0.0009 0.0006 0.0017 0.0041 0.0197 0.0008 0.0016 0.0006 0.0013
Peroxisomes 0.028 0.0009 0.0121 0.0036 0.003 0.0017 0.0135 0.0037 0.0107 0.0091 0.032 0.0035 0.0368 0.0001 0.0146 0.0027 0.0857 0.0026
Punctate Nuclear 0.0065 0.0025 0.0023 0.0012 0.004 0.0031 0.0157 0.001 0.0053 0.0094 0.006 0.0083 0.0072 0.0007 0.0011 0.0019 0.0007 0.0023
Vacuole 0.0061 0.0004 0.004 0.0015 0.0021 0.001 0.0018 0.0008 0.0008 0.0045 0.0004 0.0006 0.0013 0.0005 0.0044 0.0014 0.0007 0.0013
Vacuole Periphery 0.0023 0.0001 0.0012 0.0004 0.0013 0.0005 0.0008 0.0001 0.0002 0.0026 0.0002 0.0004 0.0002 0.0001 0.0004 0.0009 0.0002 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.2602 26.6074 29.6158 27.4568 17.9171 23.3733 36.9913 35.0181 35.0351 31.8179
Translational Efficiency 0.5662 0.6102 0.4815 0.7171 0.8137 0.7345 0.4796 0.6552 0.6031 0.574

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1681 631 2473 1023 1681 1265 221 1154 3362 1896 2694 2177

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 667.64 679.92 767.10 826.63 646.11 700.33 918.25 935.06 656.88 693.54 779.50 884.11
Standard Deviation 79.11 107.16 105.58 340.31 79.98 103.25 140.63 1609.63 80.27 105.01 116.51 1196.14
Intensity Change Log 2 — 0.026295 0.200344 0.308171 — 0.116255 0.507107 0.533279 — 0.071239 0.359357 0.423269

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000153 0.000904 0.000302 0.000390 0.000182 0.000302 0.000393 0.005080 0.000168 0.000502 0.000310 0.002876
Bud Neck 0.020364 0.032317 0.009765 0.007710 0.015762 0.032635 0.001586 0.009213 0.018063 0.032529 0.009094 0.008507
Bud Site 0.006118 0.018498 0.018630 0.024203 0.004506 0.011408 0.002370 0.048814 0.005312 0.013767 0.017296 0.037249
Cell Periphery 0.000156 0.000184 0.000127 0.000214 0.000148 0.000199 0.000202 0.000709 0.000152 0.000194 0.000134 0.000476
Cytoplasm 0.444568* 0.336084* 0.523812* 0.514352* 0.478225* 0.436982* 0.311186* 0.536566* 0.461396* 0.403403* 0.506370* 0.526127*
Cytoplasmic Foci 0.277538* 0.314170* 0.011662 0.042540 0.236924* 0.237971* 0.030525 0.023538 0.257231* 0.263331* 0.013210 0.032467
Eisosomes 0.000212 0.000189 0.000023 0.000063 0.000155 0.000186 0.000084 0.000040 0.000184 0.000187 0.000028 0.000051
Endoplasmic Reticulum 0.000874 0.000901 0.001427 0.002394 0.000862 0.000948 0.004699 0.001317 0.000868 0.000932 0.001696 0.001823
Endosome 0.015387 0.044384 0.001902 0.008138 0.011956 0.022843 0.004881 0.010727 0.013671 0.030012 0.002146 0.009511
Golgi 0.004638 0.011540 0.000862 0.001830 0.002960 0.003413 0.000196 0.005171 0.003799 0.006118 0.000808 0.003601
Lipid Particles 0.013840 0.012354 0.000666 0.001596 0.009839 0.008608 0.002752 0.000860 0.011839 0.009855 0.000837 0.001206
Mitochondria 0.009707 0.024493 0.000931 0.004589 0.010857 0.008763 0.000434 0.003590 0.010282 0.013998 0.000890 0.004059
Mitotic Spindle 0.001889 0.007476 0.007889 0.038820 0.002054 0.003752 0.001203 0.041942 0.001972 0.004991 0.007340 0.040475
None 0.003653 0.004500 0.003321 0.004763 0.003320 0.003331 0.007516 0.006853 0.003486 0.003720 0.003666 0.005871
Nuclear Periphery 0.000437 0.000505 0.001645 0.001261 0.000521 0.000463 0.001769 0.000958 0.000479 0.000477 0.001655 0.001100
Nuclear Periphery Foci 0.000260 0.000747 0.000395 0.001337 0.000346 0.000528 0.002263 0.000911 0.000303 0.000601 0.000549 0.001111
Nucleolus 0.001092 0.004388 0.000360 0.001162 0.001711 0.001818 0.000955 0.000845 0.001402 0.002673 0.000409 0.000994
Nucleus 0.142328* 0.091171 0.371063* 0.190865* 0.168992* 0.142652* 0.523848* 0.184356* 0.155660* 0.125519 0.383597* 0.187414*
Peroxisomes 0.009442 0.030629 0.000304 0.003103 0.006792 0.013858 0.000531 0.003524 0.008117 0.019439 0.000322 0.003326
Vacuole 0.046146 0.061026 0.044500 0.145603 0.042166 0.067138 0.102318 0.110267 0.044156 0.065104 0.049243 0.126872
Vacuole Periphery 0.001197 0.003540 0.000412 0.005067 0.001722 0.002203 0.000290 0.004719 0.001460 0.002648 0.000402 0.004882

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.55 -1.48 -6.22 1.75 -0.93 -1.67 -3.90 -2.49 -2.32 -1.90 -3.15 -1.64 -2.79 -1.81 -2.32
Bud Neck -3.47 6.55 8.99 8.51 3.04 -6.28 11.60 6.72 10.41 -5.77 -6.76 7.75 11.15 13.47 2.45
Bud Site -5.57 -4.61 -4.61 0.95 -0.43 -4.91 1.42 -6.95 -4.02 -7.33 -6.91 -5.35 -8.28 -3.03 -2.68
Cell Periphery -1.44 2.68 -0.17 1.28 -2.72 -2.87 -2.13 -2.08 -1.36 -1.47 -3.09 2.51 -2.07 -1.00 -2.64
Cytoplasm 8.45 -3.55 1.30 -6.61 4.26 4.25 13.36 4.43 0.35 -9.97 7.45 0.54 3.99 -3.17 3.23
Cytoplasmic Foci -3.48 47.81 42.90 31.40 -8.44 -0.16 33.85 41.85 36.35 1.17 -0.99 63.58 59.84 46.84 -7.85
Eisosomes 1.28 15.03 11.92 9.60 -12.27 -2.20 5.12 14.66 11.61 6.29 -0.27 20.90 17.70 14.58 -10.83
Endoplasmic Reticulum -0.17 -5.98 -7.00 -7.42 -3.54 -0.27 -8.15 -8.24 -3.39 6.59 -0.29 -10.86 -9.93 -6.33 -0.16
Endosome -7.84 13.56 9.36 11.08 -4.08 -5.24 7.20 7.23 9.13 0.63 -8.72 17.62 11.75 14.13 -4.60
Golgi -4.24 6.29 6.27 7.08 -0.39 -0.80 9.09 2.16 2.55 -2.71 -3.39 7.47 5.80 7.38 -1.54
Lipid Particles 0.75 12.79 11.96 6.91 -9.34 1.15 9.39 13.31 8.20 5.56 1.98 17.90 17.25 10.64 -5.96
Mitochondria -3.66 6.03 4.53 5.91 -1.35 1.00 6.06 5.37 5.15 -3.65 -1.84 8.36 7.06 7.62 -2.10
Mitotic Spindle -2.60 -4.90 -6.31 -4.12 -4.45 -1.43 -0.76 -7.39 -6.43 -6.82 -2.77 -5.37 -9.69 -7.72 -6.81
None -0.80 0.34 -1.32 -0.18 -2.61 -0.02 -2.01 -5.11 -3.50 -0.31 -0.39 -0.31 -4.03 -2.75 -5.18
Nuclear Periphery -0.64 -18.57 -13.86 -10.19 2.68 0.62 -11.67 -12.18 -13.62 4.26 -0.03 -19.93 -18.55 -17.14 4.68
Nuclear Periphery Foci -3.99 -5.70 -9.74 -4.21 -7.49 -1.43 -5.71 -9.38 -4.16 4.39 -3.27 -7.10 -13.42 -6.05 -5.57
Nucleolus -3.49 6.03 1.71 3.80 -2.48 -0.26 2.67 3.74 3.17 1.77 -2.97 6.11 4.14 4.86 -2.33
Nucleus 7.45 -37.69 -10.48 -15.02 20.10 4.15 -16.03 -8.11 -11.09 12.61 6.43 -40.89 -13.16 -17.16 24.93
Peroxisomes -6.90 12.05 8.09 9.67 -1.70 -5.05 8.86 6.31 9.68 -1.52 -8.14 15.00 10.29 13.50 -2.46
Vacuole -3.59 -7.43 -24.77 -21.09 -21.37 -7.59 -9.04 -25.03 -20.74 -3.31 -8.12 -11.54 -35.17 -29.50 -26.66
Vacuole Periphery -5.24 8.64 -1.04 1.75 -2.22 -0.99 4.87 0.28 1.06 -2.91 -3.69 6.93 -0.67 1.68 -3.38
ORF
Human Ortholog
Description Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
Localization
Cell Percentages cytoplasm (23%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YPR127W

YPR127W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ypr127w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available