Standard name
Human Ortholog
Description Nuclear encoded protein needed for splicing of mitochondrial intron; required for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0.05 0.06 0.06 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.84 0.84 0.95 0.93 0.91 0.79 0.73 0.74 0.51 0.49 0.37 0.45 0.98 0.97 0.98 – – – 0.45 0.58 0.42 0.58 0.56 0.52
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.14 0.19 0 0 0.12 0.17 0.2 0.12 0.64 0.54 0.72 0.58 0 0 0 – – – 0.28 0.24 0.42 0.27 0.25 0.26
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0.06 0.11 0 0 0 0.08 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.07 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 4 0 1 0 0 0 0 0 0 1 0 0 0 – – – 2 1 4 3 10 9
Bud 1 2 1 1 2 2 1 3 5 8 10 11 0 0 1 – – – 1 0 0 3 7 8
Bud Neck 0 0 6 2 2 3 10 8 1 1 1 2 2 1 1 – – – 0 1 0 1 3 7
Bud Site 0 0 0 0 0 1 0 1 1 1 3 1 0 0 0 – – – – – – – – –
Cell Periphery 0 1 3 4 0 1 2 1 0 1 0 0 1 1 1 – – – 0 1 1 2 4 3
Cytoplasm 81 97 127 117 86 133 164 162 77 72 58 87 249 387 409 – – – 76 80 65 151 197 188
Endoplasmic Reticulum 3 1 1 2 1 4 1 2 0 0 0 1 1 2 2 – – – 4 0 1 2 0 2
Endosome 0 0 2 1 0 1 5 3 0 0 0 0 2 3 2 – – – 9 2 4 2 6 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 – – – 2 0 1 0 2 2
Mitochondria 13 22 0 3 11 28 45 27 96 80 112 113 2 0 1 – – – 47 33 66 72 86 94
Nucleus 0 0 1 0 0 2 3 5 0 3 3 2 2 1 1 – – – 1 0 0 0 3 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 2
Nucleolus 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 1 0 1
Peroxisomes 0 0 0 0 0 0 0 0 1 0 1 0 0 0 1 – – – 0 0 0 0 0 0
SpindlePole 0 0 1 5 2 5 13 25 0 4 6 16 0 4 0 – – – 5 1 2 6 11 14
Vac/Vac Membrane 6 0 0 0 1 1 6 4 2 0 1 1 3 2 3 – – – 11 8 2 4 9 11
Unique Cell Count 96 115 134 126 94 168 224 219 150 148 156 194 255 400 417 171 139 157 264 354 362
Labelled Cell Count 104 124 146 135 107 181 250 241 183 170 195 235 262 403 422 171 139 157 264 354 362


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.2 4.8 4.9 3.7 3.8 4.3 4.4 3.3 3.6 3.3 3.3 5.0 5.0 5.0 3.8 4.6 4.7 5.6 6.2 5.9
Std Deviation (1e-4) 0.8 0.4 1.0 3.5 1.1 1.1 2.0 1.6 1.3 1.4 1.2 0.9 1.4 1.5 1.8 0.9 1.0 0.9 1.7 2.2 1.6
Intensity Change (Log2) – – – 0.04 -0.35 -0.31 -0.13 -0.1 -0.55 -0.39 -0.54 -0.54 0.07 0.08 0.07 -0.33 -0.06 -0.01 0.23 0.37 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.659 -0.6771 -0.4985 -0.7859 -0.6075 -0.4951 3.449 3.6586 3.8096 4.1901 3.4861 3.6705 0.4407 1.0688 0.892 0.7098 0.924 0.8611
Actin 0.0352 0.0069 0.0167 0.0059 0.0093 0.0055 0.0341 0.0062 0.0121 0.0182 0.0028 0.0071 0.0115 0.0019 0.0143 0.001 0.0005 0.0129
Bud 0.004 0.0026 0.0007 0.0036 0.0176 0.0005 0.0022 0.007 0.0028 0.0013 0.0008 0.0015 0.0006 0.005 0.0011 0.0014 0.0001 0.0031
Bud Neck 0.0015 0.0004 0.0012 0.0002 0.0006 0.0041 0.0037 0.001 0.0011 0.0021 0.0013 0.0055 0.0008 0.0004 0.0005 0.0011 0.0002 0.0097
Bud Periphery 0.0069 0.0019 0.0019 0.0142 0.0485 0.0011 0.0054 0.0192 0.0065 0.0033 0.0014 0.0049 0.0007 0.0092 0.0031 0.0036 0.0001 0.0086
Bud Site 0.0031 0.0065 0.0064 0.0003 0.0015 0.0004 0.0117 0.0314 0.0082 0.0078 0.0011 0.0023 0.019 0.0074 0.0015 0.0022 0.0001 0.0006
Cell Periphery 0.0007 0.0005 0.001 0.0002 0.0003 0.0003 0.0015 0.0013 0.0006 0.0008 0.0003 0.0004 0.0008 0.0011 0.0005 0.0007 0.0001 0.0009
Cytoplasm 0.0858 0.1557 0.1227 0.1165 0.1015 0.1671 0.1093 0.2239 0.1366 0.122 0.17 0.2232 0.0875 0.2509 0.1015 0.115 0.1249 0.2149
Cytoplasmic Foci 0.0986 0.0803 0.0648 0.0392 0.0754 0.0785 0.123 0.0493 0.0938 0.0505 0.1178 0.1049 0.1026 0.0407 0.0567 0.0699 0.0236 0.0558
Eisosomes 0.001 0.0011 0.0011 0.0002 0.0003 0.0005 0.0008 0.0004 0.0005 0.0009 0.0006 0.0002 0.0005 0.0004 0.0005 0.0001 0 0.0003
Endoplasmic Reticulum 0.0059 0.0023 0.0044 0.0018 0.0013 0.0031 0.0033 0.0032 0.0026 0.004 0.0033 0.0017 0.0058 0.0032 0.0019 0.003 0.0033 0.0055
Endosome 0.057 0.0458 0.0521 0.0027 0.0604 0.0516 0.0549 0.0312 0.043 0.0792 0.0622 0.0486 0.0341 0.0184 0.0317 0.0889 0.0624 0.0231
Golgi 0.009 0.0081 0.0071 0.0008 0.0186 0.0099 0.0104 0.0081 0.0117 0.0054 0.0083 0.0103 0.0079 0.0025 0.0136 0.0124 0.0277 0.0031
Lipid Particles 0.0239 0.0039 0.0216 0.0031 0.0012 0.0086 0.0174 0.0013 0.0047 0.0087 0.0051 0.0061 0.0258 0.0021 0.0046 0.002 0.0077 0.0019
Mitochondria 0.0152 0.0056 0.0256 0.0012 0.0865 0.0348 0.0289 0.0397 0.0341 0.0365 0.0166 0.0284 0.0062 0.0046 0.0597 0.066 0.0254 0.0133
None 0.6081 0.6598 0.6336 0.7093 0.5488 0.6066 0.5534 0.562 0.6039 0.6067 0.5713 0.5264 0.6574 0.6432 0.6589 0.6039 0.7192 0.6294
Nuclear Periphery 0.0119 0.001 0.0036 0.0012 0.0005 0.0045 0.0047 0.0006 0.0031 0.0039 0.0099 0.0008 0.0047 0.0006 0.0023 0.0013 0.0003 0.0006
Nucleolus 0.0008 0.0006 0.0013 0.0003 0.0005 0.0006 0.0011 0.0006 0.0009 0.0026 0.0008 0.0004 0.0012 0.0003 0.0005 0.0006 0.0001 0.0002
Nucleus 0.004 0.0017 0.0021 0.0011 0.0011 0.0015 0.0022 0.0015 0.0018 0.0059 0.0098 0.0009 0.0039 0.0007 0.0013 0.0012 0.0001 0.0006
Peroxisomes 0.0113 0.0062 0.0165 0.0936 0.0174 0.0112 0.0221 0.0053 0.0243 0.0275 0.0077 0.0167 0.0158 0.0027 0.0327 0.0071 0.0014 0.013
Punctate Nuclear 0.0081 0.0021 0.0033 0.0032 0.0027 0.0047 0.0029 0.0007 0.0029 0.0056 0.0021 0.0055 0.007 0.0004 0.0007 0.0009 0.0002 0.001
Vacuole 0.0062 0.006 0.0094 0.0011 0.0045 0.0033 0.0056 0.005 0.0037 0.005 0.005 0.0031 0.0052 0.0039 0.0072 0.0125 0.0015 0.0012
Vacuole Periphery 0.0018 0.001 0.0027 0.0003 0.0014 0.0016 0.0014 0.001 0.0012 0.0022 0.0017 0.0009 0.0009 0.0005 0.0052 0.0052 0.001 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.1528 2.4446 6.3489 8.9673 6.2046 4.0996 9.2688 10.8434 10.885 7.778
Translational Efficiency 0.3995 1.5419 0.7468 0.9088 0.7875 0.6201 0.4799 0.6468 0.7164 0.9138

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
34 1309 1104 2121 1762 2233 546 1824 1796 3542 1650 3945

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 610.66 737.39 774.46 816.65 663.33 796.45 790.15 799.18 662.33 774.62 779.65 808.57
Standard Deviation 78.36 84.87 105.66 184.55 74.95 91.21 91.68 105.76 75.36 93.38 101.52 153.49
Intensity Change Log 2 — 0.272059 0.342821 0.419349 — 0.263857 0.252400 0.268794 — 0.267794 0.296449 0.342921

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000537 0.001067 0.000423 0.000936 0.000118 0.000486 0.000161 0.002105 0.000126 0.000701 0.000336 0.001477
Bud Neck 0.019381 0.056181 0.004585 0.021386 0.021321 0.072632 0.004533 0.015254 0.021284 0.066552 0.004568 0.018551
Bud Site 0.019846 0.027413 0.005370 0.047305 0.004653 0.027255 0.002658 0.034946 0.004941 0.027313 0.004472 0.041591
Cell Periphery 0.000779 0.000224 0.000136 0.000164 0.000137 0.000247 0.000221 0.000179 0.000149 0.000239 0.000164 0.000171
Cytoplasm 0.280469* 0.232200* 0.368705* 0.412536* 0.406836* 0.320555* 0.367657* 0.447865* 0.404444* 0.287902* 0.368358* 0.428871*
Cytoplasmic Foci 0.266436* 0.336317* 0.025431 0.045253 0.312585* 0.280367* 0.072235 0.075871 0.311712* 0.301044* 0.040919 0.059410
Eisosomes 0.000579 0.000243 0.000042 0.000056 0.000181 0.000287 0.000109 0.000078 0.000189 0.000271 0.000065 0.000066
Endoplasmic Reticulum 0.001560 0.000916 0.003988 0.001905 0.001336 0.000717 0.001662 0.001869 0.001340 0.000791 0.003219 0.001888
Endosome 0.016772 0.052981 0.003488 0.012580 0.014417 0.037979 0.010450 0.008238 0.014461 0.043523 0.005792 0.010572
Golgi 0.023934 0.020960 0.000345 0.004249 0.006066 0.011995 0.000331 0.003128 0.006404 0.015308 0.000340 0.003731
Lipid Particles 0.049083 0.014354 0.002311 0.002410 0.015834 0.012961 0.005653 0.002964 0.016464 0.013476 0.003417 0.002666
Mitochondria 0.091443 0.045843 0.000716 0.010112 0.013096 0.034380 0.001195 0.004283 0.014580 0.038616 0.000874 0.007417
Mitotic Spindle 0.002135 0.005472 0.003968 0.078086 0.000686 0.008219 0.003915 0.019118 0.000714 0.007204 0.003950 0.050822
None 0.007828 0.002108 0.004780 0.004532 0.003295 0.002269 0.008533 0.005164 0.003381 0.002209 0.006022 0.004824
Nuclear Periphery 0.000399 0.000485 0.001821 0.002248 0.000572 0.000335 0.001423 0.001315 0.000569 0.000390 0.001689 0.001817
Nuclear Periphery Foci 0.000152 0.000313 0.001540 0.001136 0.000325 0.000223 0.001085 0.000837 0.000321 0.000256 0.001390 0.000998
Nucleolus 0.002174 0.003034 0.001072 0.001431 0.001658 0.002090 0.003136 0.001457 0.001668 0.002439 0.001755 0.001443
Nucleus 0.080493 0.084095 0.526686* 0.213523* 0.144460* 0.090632 0.434767* 0.239646* 0.143249* 0.088216 0.496270* 0.225601*
Peroxisomes 0.119921* 0.042879 0.001020 0.010891 0.011237 0.024606 0.002436 0.022868 0.013295 0.031359 0.001488 0.016429
Vacuole 0.013480 0.067763 0.043232 0.125207 0.039322 0.068201 0.077436 0.111068 0.038833 0.068039 0.054551 0.118670
Vacuole Periphery 0.002601 0.005152 0.000339 0.004053 0.001863 0.003564 0.000404 0.001746 0.001877 0.004151 0.000361 0.002986

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.88 0.12 -1.36 1.14 -2.48 -5.35 -1.96 -4.50 -3.32 -4.16 -6.45 -4.64 -6.64 -2.64 -4.46
Bud Neck -5.65 2.50 0.19 11.95 -11.63 -16.21 11.19 3.61 18.95 -8.97 -17.98 11.43 2.95 22.28 -14.15
Bud Site -0.84 1.62 -1.15 -0.97 -10.23 -12.15 2.90 -9.95 0.16 -10.75 -15.64 0.07 -14.89 -0.64 -14.05
Cell Periphery 2.59 2.94 3.03 5.55 1.56 -6.35 -5.67 -1.06 5.08 4.13 -6.56 -2.18 1.16 7.45 3.40
Cytoplasm 0.99 -1.19 -1.80 -15.27 -2.85 10.15 4.63 -0.09 -9.98 -4.63 15.30 6.92 2.38 -15.69 -5.60
Cytoplasmic Foci -2.50 8.97 8.51 44.88 -4.74 4.45 33.11 39.42 38.56 0.71 1.73 45.89 45.25 59.53 -4.02
Eisosomes 3.26 5.10 5.03 17.11 -2.26 -3.52 5.36 9.69 7.39 5.02 -3.90 10.80 11.55 11.12 1.38
Endoplasmic Reticulum 0.94 -5.02 -1.25 -11.28 8.82 2.89 -3.14 -4.66 -13.56 -0.18 2.65 -9.99 -5.03 -17.49 8.11
Endosome -7.09 2.89 1.94 17.23 -5.51 -13.07 2.58 8.35 18.93 2.02 -18.08 8.76 7.90 25.18 -2.21
Golgi 0.36 2.62 2.41 10.80 -3.87 -5.37 8.84 5.18 10.08 -4.01 -8.28 9.13 5.86 14.72 -5.45
Lipid Particles 2.38 3.19 3.21 11.18 0.78 2.24 6.10 12.30 11.79 1.81 2.48 11.20 13.09 16.37 1.81
Mitochondria 1.87 3.70 3.47 11.88 -8.60 -8.89 8.86 7.16 14.96 -3.42 -10.79 10.05 6.77 18.67 -9.26
Mitotic Spindle -2.28 -1.61 -13.59 -12.69 -12.57 -6.27 -1.69 -8.80 -4.59 -5.90 -7.34 -3.39 -16.67 -12.96 -13.67
None 1.01 0.65 0.72 -2.34 0.58 2.14 -2.83 -1.06 -3.01 2.28 2.35 -2.70 -0.91 -3.88 2.08
Nuclear Periphery -0.60 -9.03 -12.73 -16.51 -5.91 3.05 -8.19 -12.31 -18.40 -1.55 2.27 -13.30 -17.72 -22.92 -5.20
Nuclear Periphery Foci -3.92 -8.83 -4.21 -3.37 3.56 2.12 -5.94 -5.67 -7.29 1.44 1.58 -9.07 -5.17 -6.36 4.05
Nucleolus -0.80 0.63 0.60 4.15 -0.18 -1.29 -1.59 0.01 0.85 1.52 -2.61 -0.49 0.71 3.43 0.89
Nucleus -0.17 -18.12 -7.42 -26.35 26.55 11.46 -22.80 -17.23 -26.63 13.16 12.65 -44.07 -22.07 -37.82 29.41
Peroxisomes 3.05 4.73 4.48 15.03 -6.58 -9.11 8.49 -2.55 3.91 -7.42 -11.93 11.33 1.38 13.59 -10.07
Vacuole -12.54 -9.99 -30.36 -21.64 -21.89 -10.27 -8.83 -24.90 -17.89 -8.00 -12.27 -9.70 -37.04 -28.30 -22.53
Vacuole Periphery -2.16 2.15 0.44 4.77 -4.52 -6.05 10.23 1.51 4.91 -2.00 -8.15 10.67 0.60 6.59 -4.69
Standard name
Human Ortholog
Description Nuclear encoded protein needed for splicing of mitochondrial intron; required for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions
Localization
Cell Percentages cytoplasm (20%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Mss18

Mss18


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mss18-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available