Standard name
Human Ortholog
Description Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.66 0.92 0.94 0.92 0.84 0.75 0.59 0.56 0.5 0.4 0.41 0.39 0.83 0.91 0.91 0.76 0.68 0.67 0.32 0.58 0.53 0.36 0.47 0.46
Endoplasmic Reticulum 0.05 0.05 0 0 0.05 0 0 0 0 0 0 0 0.13 0.06 0 0.05 0.05 0.08 0.29 0.14 0.2 0.29 0.17 0.14
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.11 0.08 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.39 0 0.07 0.11 0.16 0.44 0.53 0.57 0.66 0.72 0.73 0.76 0 0 0 0.05 0.12 0 0.21 0.15 0.17 0.2 0.15 0.18
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.11 0.12 0 0 0 0.06 0.11 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 1 2 2 5 3 3 4
Bud 2 0 2 1 2 3 12 6 7 10 9 5 0 0 0 0 2 1 0 0 1 0 1 2
Bud Neck 0 0 2 0 0 1 1 1 0 5 0 0 0 1 0 0 0 1 1 5 1 1 2 5
Bud Site 1 0 0 0 0 0 0 2 6 2 5 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 2 3 3 0 0 1 0 4 2 0 3 2 4 1 0 1 1 2 7 2 1 2
Cytoplasm 96 182 304 196 239 271 302 286 218 153 143 147 153 204 202 48 67 16 59 133 188 47 92 108
Endoplasmic Reticulum 7 9 2 5 14 1 11 1 7 6 1 14 24 13 5 3 5 2 54 31 71 38 32 32
Endosome 0 3 2 1 2 0 0 0 1 0 1 0 2 1 8 5 11 2 4 4 1 1 2 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0 1 0 0 3 1
Mitochondria 57 4 23 23 47 158 270 292 287 273 251 288 1 0 0 3 12 1 38 35 62 25 28 41
Nucleus 0 0 0 0 0 2 4 4 1 1 4 3 4 0 0 0 0 0 1 0 0 0 0 0
Nuclear Periphery 0 0 0 0 1 1 1 0 3 2 1 2 0 0 0 0 0 1 2 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 0 1 0 2 1 0 0 1 0 0 1 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 3 0 0 1 0 3 3 4 1 3 0 2 0 0 2 2 1 1 2 0 1 3
Vac/Vac Membrane 3 3 1 1 7 7 12 11 9 7 9 10 5 6 11 4 11 3 6 8 6 7 21 24
Unique Cell Count 146 198 325 214 285 359 508 509 435 381 346 380 185 223 222 63 98 24 184 231 359 132 197 236
Labelled Cell Count 168 201 341 230 315 446 615 607 543 467 429 474 192 230 233 65 110 32 184 231 359 132 197 236


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 4.4 3.4 3.5 3.5 3.3 2.8 3.0 2.8 2.6 2.8 2.8 5.8 5.3 6.7 5.6 5.5 6.2 6.0 6.2 6.4
Std Deviation (1e-4) 1.5 0.9 0.9 1.0 0.8 0.9 0.5 0.7 0.7 0.4 0.7 0.6 1.0 1.0 13.8 0.9 1.0 1.6 1.1 1.4 1.6
Intensity Change (Log2) – – – 0.04 0.02 -0.04 -0.27 -0.21 -0.29 -0.38 -0.31 -0.27 0.76 0.62 0.95 0.71 0.68 0.86 0.8 0.84 0.89


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.4883 2.4871 2.2358 2.2246 2.4363 2.2646 4.3753 4.5245 4.5984 4.0679 3.979 4.4852 2.199 2.6435 1.9703 1.8551 2.0389 1.9534
Actin 0.0294 0.0005 0.0129 0.0081 0.0159 0.0056 0.0068 0.0009 0.0025 0.0016 0.0011 0.0035 0.0005 0.0004 0.001 0.0005 0.0015 0.0028
Bud 0.001 0.0001 0.0032 0.0007 0.0004 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0.0001
Bud Neck 0.0055 0.0004 0.0049 0.0007 0.0011 0.001 0.0007 0.0003 0.0006 0.0011 0.0005 0.0015 0.0005 0.0002 0.0004 0.0003 0.0209 0.0015
Bud Periphery 0.0016 0.0001 0.0017 0.0011 0.001 0.0004 0.0004 0.0001 0.0002 0.0003 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001
Bud Site 0.0131 0.0039 0.0085 0.0013 0.0018 0.0003 0.0018 0.0015 0.0009 0.0002 0.0001 0.0003 0.0012 0.0004 0.0009 0.0001 0.0011 0.0002
Cell Periphery 0.0018 0.001 0.0011 0.0007 0.0005 0.0005 0.0012 0.0008 0.0011 0.0018 0.0006 0.0008 0.0008 0.0006 0.0006 0.0006 0.0005 0.0005
Cytoplasm 0.0609 0.1866 0.124 0.0784 0.1667 0.1439 0.0514 0.0807 0.0887 0.0464 0.1277 0.0901 0.0645 0.109 0.0726 0.0943 0.0938 0.0913
Cytoplasmic Foci 0.0485 0.0439 0.0513 0.0703 0.0337 0.0285 0.0319 0.0528 0.0501 0.0426 0.0243 0.0378 0.0451 0.0406 0.0511 0.0282 0.0446 0.0442
Eisosomes 0.0006 0 0.0002 0.0003 0.0005 0.0002 0.0004 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0.0001
Endoplasmic Reticulum 0.3794 0.3558 0.4075 0.3787 0.2336 0.3992 0.4605 0.3713 0.4 0.6044 0.5267 0.469 0.3136 0.4112 0.3551 0.4917 0.3903 0.382
Endosome 0.1491 0.1476 0.1411 0.1712 0.1494 0.1197 0.1187 0.1741 0.1614 0.099 0.1264 0.1132 0.1876 0.1763 0.1596 0.1607 0.1543 0.1537
Golgi 0.0153 0.0112 0.0145 0.0267 0.0135 0.0102 0.0113 0.017 0.0161 0.0117 0.0095 0.0099 0.0105 0.0131 0.0144 0.023 0.0117 0.0109
Lipid Particles 0.1513 0.1164 0.1006 0.0733 0.0648 0.1299 0.2041 0.1873 0.176 0.0899 0.0627 0.1761 0.2491 0.1553 0.2319 0.102 0.1215 0.1834
Mitochondria 0.0124 0.0019 0.0088 0.048 0.0162 0.0109 0.0038 0.0013 0.0035 0.0092 0.0019 0.0076 0.0031 0.0035 0.0037 0.0045 0.0058 0.0045
None 0.0232 0.0302 0.0257 0.0188 0.1582 0.0443 0.039 0.0496 0.0377 0.0046 0.0512 0.0234 0.0151 0.0185 0.033 0.0147 0.042 0.0246
Nuclear Periphery 0.0434 0.0572 0.0506 0.0451 0.0475 0.0649 0.038 0.0358 0.0309 0.0477 0.0405 0.0362 0.0651 0.0435 0.0483 0.053 0.0786 0.0605
Nucleolus 0.0075 0.0008 0.0006 0.0009 0.0049 0.0004 0.0004 0.0003 0.0004 0.0002 0.0002 0.0003 0.0015 0.0002 0.0003 0.0003 0.0006 0.0008
Nucleus 0.0093 0.0065 0.0034 0.0135 0.0152 0.0082 0.0027 0.0019 0.0027 0.0031 0.0042 0.0045 0.0054 0.0033 0.003 0.004 0.0084 0.0107
Peroxisomes 0.0055 0.0015 0.0107 0.0188 0.04 0.0018 0.0022 0.0011 0.0036 0.0045 0.0008 0.0033 0.0018 0.001 0.003 0.001 0.0064 0.0027
Punctate Nuclear 0.0098 0.0073 0.0103 0.0271 0.0187 0.0077 0.0066 0.0069 0.0043 0.0011 0.0034 0.0059 0.0091 0.0026 0.0036 0.0066 0.0043 0.0076
Vacuole 0.0271 0.0235 0.0148 0.0116 0.0122 0.0177 0.0157 0.0142 0.0167 0.0257 0.0161 0.0132 0.0214 0.0168 0.0138 0.0113 0.0104 0.0145
Vacuole Periphery 0.0044 0.0037 0.0036 0.0046 0.0039 0.0044 0.0024 0.0019 0.0024 0.0051 0.0018 0.003 0.0037 0.0033 0.0034 0.003 0.003 0.0034

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 43.3786 32.77 25.1273 41.0028 43.5679 36.6377 31.477 32.0536 26.2649 49.1777
Translational Efficiency 1.1777 0.9936 1.2447 0.7847 0.9128 1.1762 1.0008 0.7932 1.0357 0.9031

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1846 2046 1706 152 1717 2051 3344 1753 3563 4097 5050 1905

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 678.14 776.87 816.53 847.33 711.88 737.16 882.06 798.85 694.40 756.99 859.92 802.72
Standard Deviation 73.58 91.32 93.54 105.41 90.59 91.88 106.66 97.85 83.93 93.73 107.00 99.34
Intensity Change Log 2 — 0.196090 0.267923 0.321341 — 0.050344 0.309243 0.166291 — 0.123288 0.289232 0.244016

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000101 0.000295 0.000140 0.000336 0.000433 0.000196 0.000222 0.000105 0.000261 0.000246 0.000194 0.000124
Bud Neck 0.008877 0.015148 0.003400 0.005880 0.010775 0.013675 0.012119 0.005507 0.009792 0.014410 0.009174 0.005537
Bud Site 0.002133 0.005243 0.009879 0.011502 0.002977 0.006742 0.008103 0.007071 0.002539 0.005993 0.008703 0.007424
Cell Periphery 0.000181 0.000131 0.000192 0.000293 0.000176 0.000240 0.000158 0.000138 0.000178 0.000185 0.000170 0.000151
Cytoplasm 0.406913* 0.331792* 0.521873* 0.310903* 0.391567* 0.337803* 0.499708* 0.600297* 0.399518* 0.334801* 0.507196* 0.577206*
Cytoplasmic Foci 0.212024* 0.229086* 0.030831 0.092907 0.217553* 0.228496* 0.032053 0.078447 0.214688* 0.228791* 0.031640 0.079600
Eisosomes 0.000285 0.000260 0.000047 0.000163 0.000361 0.000294 0.000056 0.000061 0.000322 0.000277 0.000053 0.000069
Endoplasmic Reticulum 0.010870 0.025536 0.003034 0.010483 0.023916 0.027640 0.017497 0.002365 0.017157 0.026589 0.012611 0.003013
Endosome 0.014911 0.025172 0.003969 0.020250 0.021436 0.036721 0.004316 0.006750 0.018056 0.030954 0.004198 0.007827
Golgi 0.003028 0.008190 0.000163 0.001588 0.005552 0.009276 0.000138 0.000165 0.004244 0.008733 0.000147 0.000279
Lipid Particles 0.048071 0.079679 0.002580 0.012116 0.076520 0.103011* 0.007846 0.005437 0.061781 0.091359 0.006067 0.005970
Mitochondria 0.007277 0.012972 0.000513 0.000379 0.006595 0.012909 0.001143 0.000292 0.006948 0.012940 0.000930 0.000299
Mitotic Spindle 0.001907 0.002076 0.003802 0.011560 0.001629 0.006663 0.004214 0.000678 0.001773 0.004372 0.004075 0.001547
None 0.002266 0.002653 0.004706 0.001798 0.002732 0.003391 0.001719 0.002958 0.002490 0.003022 0.002728 0.002866
Nuclear Periphery 0.003186 0.004766 0.002657 0.003018 0.005025 0.005912 0.013167 0.001672 0.004072 0.005340 0.009616 0.001780
Nuclear Periphery Foci 0.001229 0.003372 0.003638 0.012478 0.002419 0.003304 0.008421 0.003347 0.001803 0.003338 0.006805 0.004076
Nucleolus 0.001547 0.002660 0.000692 0.002715 0.001897 0.002073 0.000828 0.000795 0.001716 0.002366 0.000782 0.000948
Nucleus 0.205434* 0.158574* 0.351809* 0.284349* 0.157986* 0.104776 0.335710* 0.218825* 0.182569* 0.131642* 0.341149* 0.224053*
Peroxisomes 0.004379 0.007197 0.000466 0.001306 0.004826 0.007419 0.000883 0.000864 0.004594 0.007308 0.000742 0.000899
Vacuole 0.064128 0.083173 0.055378 0.214583* 0.064155 0.085647 0.051513 0.063973 0.064141 0.084412 0.052819 0.075990
Vacuole Periphery 0.001254 0.002025 0.000232 0.001393 0.001470 0.003812 0.000185 0.000253 0.001358 0.002920 0.000201 0.000344

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.00 -2.44 -2.59 -1.04 -2.15 1.22 0.90 1.59 2.29 3.19 0.14 0.38 1.14 2.84 2.23
Bud Neck -6.41 7.87 2.32 6.31 -1.54 -2.02 -1.69 4.03 5.76 5.88 -5.39 0.05 4.26 8.22 4.11
Bud Site -3.46 -4.77 -1.37 -0.79 -0.04 -3.40 -4.44 -2.87 -0.10 0.62 -4.85 -6.71 -3.60 -0.83 0.86
Cell Periphery 6.82 -0.95 -2.59 -3.98 -2.16 -4.67 1.36 3.53 7.46 1.93 -0.90 0.82 3.31 3.76 2.18
Cytoplasm 9.98 -10.42 5.54 1.57 10.01 6.51 -12.56 -22.85 -29.71 -13.44 11.64 -16.34 -22.46 -31.41 -10.50
Cytoplasmic Foci -2.82 40.35 13.19 14.90 -7.05 -1.79 39.96 26.57 29.95 -15.04 -3.29 56.72 33.49 37.11 -17.01
Eisosomes 1.18 14.78 4.90 3.95 -6.02 2.27 11.11 11.01 18.79 -0.74 2.49 17.15 16.07 20.66 -4.13
Endoplasmic Reticulum -7.73 7.80 0.39 5.29 -2.60 -1.46 3.14 11.84 13.83 15.62 -5.96 3.71 13.58 18.73 13.99
Endosome -7.57 12.25 -0.70 2.05 -3.61 -8.03 14.19 11.15 18.52 -3.04 -11.07 18.47 10.75 20.46 -4.55
Golgi -7.51 7.30 1.95 6.62 -1.04 -4.29 12.43 12.36 12.21 -0.86 -8.10 14.00 13.27 17.95 -1.20
Lipid Particles -9.14 21.14 12.86 20.60 -4.36 -5.88 22.49 23.24 28.83 3.67 -10.42 29.74 29.15 38.38 0.61
Mitochondria -4.49 7.43 7.69 14.33 1.18 -4.81 7.46 8.71 11.63 12.64 -6.54 10.23 11.41 18.10 9.84
Mitotic Spindle -0.25 -1.53 -1.00 -0.97 -0.72 -3.39 -3.28 1.77 4.32 5.15 -3.03 -3.46 0.76 3.48 3.92
None -0.61 -3.08 1.33 1.62 3.87 -0.84 3.24 -0.24 0.64 -2.84 -1.05 -0.21 -0.60 0.42 -0.47
Nuclear Periphery -2.61 1.03 -0.20 2.25 -1.00 -1.29 -10.14 6.95 7.70 16.03 -2.80 -10.76 7.46 8.41 15.78
Nuclear Periphery Foci -7.41 -5.38 -5.60 -4.48 -4.23 -2.31 -9.83 -2.51 -0.87 7.16 -6.65 -11.83 -5.78 -2.28 4.72
Nucleolus -4.10 4.81 -0.64 0.53 -1.60 -0.36 4.55 5.26 4.66 1.30 -3.04 5.97 4.60 6.99 -0.09
Nucleus 8.56 -20.04 -2.39 -4.80 5.25 11.30 -31.94 -9.84 -20.26 18.13 13.89 -35.76 -7.01 -17.04 20.58
Peroxisomes -5.04 10.14 6.83 12.44 -3.57 -3.82 7.52 7.26 13.14 -0.00 -6.19 11.90 10.32 18.31 -1.24
Vacuole -5.92 -2.26 -9.97 -9.00 -9.51 -5.98 -0.37 -2.88 2.60 -2.71 -8.42 -1.56 -7.03 -1.55 -6.01
Vacuole Periphery -4.46 8.60 0.51 1.89 -1.39 -5.61 13.24 12.16 8.79 -1.80 -6.89 15.01 11.51 11.96 -1.97
Standard name
Human Ortholog
Description Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA
Localization
Cell Percentages ER (31%), cytoplasm (11%), mixed (44%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Loa1

Loa1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Loa1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available