Standard name
Human Ortholog
Description RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 – 0 0.06 0 0.06 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.07 – 0.06 – 0 0 0 0 0 0.05 0.19 0.13 0.15 0.07 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.25 0.18 – 0.4 – 0.47 0.57 0.53 0.59 0.62 0 0 0 0.06 0.12 0 0 0 0 0 0 0
Nucleus 0.86 0.77 0.8 0.81 – 0.73 – 0.64 0.65 0.67 0.62 0.63 0.74 0.66 0.76 0.61 0.76 0.75 0.78 0.69 0.74 0.59 0.48 0.4
Nuclear Periphery 0 0 0 0 – 0 – 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.29 0.35 0.32 0.17 – 0.17 – 0.22 0.33 0.27 0.24 0.27 0.37 0.24 0.14 0.23 0.26 0.25 0.14 0.23 0.2 0.31 0.35 0.34
Peroxisomes 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0.12 0 0 0 0 0.11 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 2 – 4 1 5 4 3 0 0 0 0 0 0 0 1 0 1 1 2
Bud Neck 0 0 0 0 – 1 – 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 1 0 3 1 – 3 – 11 4 17 14 11 0 1 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 2 3 10 – 16 – 9 1 11 6 3 6 52 37 10 3 1 1 0 0 1 1 2
Endoplasmic Reticulum 0 0 0 0 – 0 – 0 0 0 0 0 1 2 0 2 0 0 0 0 0 0 0 1
Endosome 0 1 0 0 – 0 – 0 0 0 0 0 0 3 6 1 0 0 3 2 1 0 3 8
Golgi 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 1 0 0 1 0 1 0 2 1
Mitochondria 13 7 51 24 – 99 – 158 39 203 141 178 2 1 1 4 5 0 2 1 0 2 1 5
Nucleus 287 150 163 111 – 181 – 216 45 257 148 181 89 184 225 40 32 12 275 154 150 95 115 105
Nuclear Periphery 0 2 6 2 – 5 – 5 5 13 6 7 0 0 1 0 0 0 0 1 0 0 1 0
Nucleolus 97 69 66 23 – 42 – 76 23 105 58 77 45 67 40 15 11 4 49 51 40 48 83 89
Peroxisomes 0 0 0 0 – 0 – 0 0 0 0 0 0 0 6 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 – 2 – 1 0 1 1 3 0 1 3 0 0 0 2 1 0 0 1 4
Vac/Vac Membrane 4 4 1 1 – 2 – 3 0 0 1 1 1 4 3 1 0 2 14 6 3 7 25 38
Unique Cell Count 334 196 204 137 249 338 69 386 239 289 121 280 295 66 42 16 353 224 202 160 241 264
Labelled Cell Count 403 235 294 172 353 483 118 613 380 464 144 315 323 75 51 19 353 224 202 160 241 264


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.9 10.5 5.5 6.1 – 4.8 – 4.6 3.9 4.2 3.8 3.9 8.9 8.7 8.6 16.5 16.8 16.5 9.5 11.2 10.4
Std Deviation (1e-4) 2.0 3.1 1.3 1.9 – 1.5 – 1.5 0.9 1.2 0.9 0.9 2.2 2.6 2.4 6.0 5.9 5.8 2.5 2.5 2.3
Intensity Change (Log2) – – – 0.15 – -0.19 – -0.26 -0.47 -0.39 -0.52 -0.49 0.7 0.68 0.65 1.59 1.62 1.6 0.8 1.03 0.93


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.8 – 2.6 – 0 0 0 0 0 0 5.9 4.5 0 0 0
Endoplasmic Reticulum 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.6 – 0 – 0 0 0 0 0 -5.5 -8.6 -8.9 -3.3 -1.8 0
Nucleus 0.3 – -1.8 – -3.9 -2.5 -3.4 -4.1 -4.1 -1.3 -3.4 -1.0 -3.2 -0.5 -0.5
Nuclear Periphery 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -3.2 – 0 – 0 0 0 0 0 0.9 -2.1 -5.0 -1.5 -0.8 -0.6
Peroxisomes 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2322 3.6052 3.1587 2.8778 2.5579 3.0108 3.1239 3.005 2.663 2.5347 2.8921 2.6444 5.3245 4.8231 4.6936 4.4861 4.0042 4.3645
Actin 0.0186 0.0001 0.0203 0.0204 0.0157 0.0011 0.0067 0 0.006 0.0032 0.011 0.0001 0.0149 0 0.0003 0.0113 0.0005 0.0001
Bud 0.0003 0 0.0002 0.0004 0.0003 0.0001 0.0003 0.0001 0.0004 0.0005 0.0016 0 0.0003 0 0 0.0001 0.0006 0
Bud Neck 0.001 0.0001 0.0006 0.0017 0.0011 0.0012 0.0021 0.0005 0.0004 0.0016 0.0009 0.0007 0.0017 0.0001 0.0002 0.0016 0.0009 0.0011
Bud Periphery 0.0008 0.0001 0.0003 0.0012 0.0008 0.0003 0.0006 0.0001 0.0003 0.0014 0.0046 0.0001 0.0006 0 0.0001 0.0002 0.0022 0.0001
Bud Site 0.0017 0.0001 0.0018 0.0021 0.0006 0.0002 0.0021 0.0043 0.0077 0.0008 0.002 0 0.0025 0 0.0001 0.0025 0.001 0.0001
Cell Periphery 0.0004 0 0.0001 0.0004 0.0002 0.0001 0.0002 0.0001 0 0.0001 0.0004 0 0.0002 0 0 0.0001 0.0004 0
Cytoplasm 0.0065 0.0001 0.0101 0.0003 0.0002 0.0004 0.0105 0.0001 0.0001 0.0013 0.0048 0 0.0015 0.0001 0.0001 0.0007 0.0001 0
Cytoplasmic Foci 0.0142 0 0.0108 0.0097 0.001 0.0011 0.0069 0 0.0017 0.0055 0.019 0.0001 0.0109 0 0.0035 0.0024 0.0002 0
Eisosomes 0.0006 0 0.0001 0.0001 0.0002 0.0001 0.0002 0 0 0.0001 0.0003 0 0.0002 0 0 0.0002 0.0001 0
Endoplasmic Reticulum 0.0036 0 0.0023 0.0002 0.0006 0.0003 0.0028 0 0.0003 0.0003 0.0025 0.0002 0.0009 0 0.0001 0.0017 0.0001 0
Endosome 0.0123 0 0.0092 0.0045 0.0091 0.0017 0.0104 0 0.0036 0.0152 0.039 0.0059 0.0073 0 0.0007 0.0029 0.0003 0.0001
Golgi 0.0031 0 0.0017 0.0075 0.0315 0.0003 0.0034 0 0.0011 0.0164 0.007 0.0014 0.0027 0 0.0004 0.0014 0.0001 0
Lipid Particles 0.0117 0 0.003 0.0092 0.0023 0.0034 0.0185 0 0.0091 0.0232 0.0369 0.0004 0.0072 0 0.0017 0.0035 0.0008 0.0003
Mitochondria 0.0109 0.0003 0.0027 0.0044 0.0125 0.0011 0.0102 0.0002 0.0005 0.0665 0.0311 0.0023 0.0055 0.0001 0.0002 0.0005 0.0363 0.0036
None 0.0193 0 0.0085 0.0003 0.0008 0.0017 0.0031 0 0.0001 0.0002 0.0014 0 0.003 0 0.0001 0.0025 0.0002 0
Nuclear Periphery 0.0117 0.0128 0.0115 0.0027 0.0132 0.0111 0.0167 0.0043 0.0049 0.0321 0.02 0.0014 0.0031 0.0181 0.024 0.01 0.0017 0.0011
Nucleolus 0.0521 0.0557 0.0559 0.075 0.3053 0.1459 0.0621 0.0631 0.076 0.1102 0.2391 0.171 0.0705 0.0611 0.0517 0.1114 0.287 0.1828
Nucleus 0.801 0.9287 0.8453 0.8134 0.5895 0.819 0.8226 0.9169 0.8811 0.6768 0.5344 0.8141 0.8423 0.9074 0.9051 0.8287 0.6538 0.8072
Peroxisomes 0.006 0 0.0054 0.0383 0.0005 0.0006 0.0042 0 0.0008 0.0057 0.0252 0 0.0071 0 0.0055 0.0012 0.0008 0
Punctate Nuclear 0.0174 0.0016 0.0076 0.0061 0.0124 0.009 0.0115 0.01 0.0049 0.0352 0.0099 0.0013 0.0137 0.0127 0.006 0.0165 0.0096 0.003
Vacuole 0.0043 0.0001 0.0015 0.0013 0.0008 0.0006 0.0029 0.0003 0.0005 0.002 0.0049 0.0003 0.0028 0 0.0001 0.0003 0.0019 0.0002
Vacuole Periphery 0.0023 0.0002 0.001 0.0009 0.0017 0.0006 0.002 0.0001 0.0003 0.0017 0.0042 0.0005 0.001 0.0001 0.0004 0.0003 0.0016 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.0278 14.2071 19.5785 15.502 23.5492 11.3456 16.9299 15.9279 17.3514 17.6229
Translational Efficiency 2.8358 3.5235 1.8933 3.1201 1.9502 4.1631 2.0829 1.9947 2.0576 2.5342

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2190 1521 1000 1222 2316 1899 2116 1977 4506 3420 3116 3199

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 808.62 856.72 1108.91 1263.37 911.08 978.83 1242.88 1188.25 861.28 924.52 1199.89 1216.95
Standard Deviation 145.17 169.22 199.92 246.32 146.64 174.26 211.77 229.11 154.65 182.42 217.24 238.64
Intensity Change Log 2 — 0.083362 0.455608 0.643743 — 0.103481 0.448037 0.383189 — 0.094056 0.451602 0.511578

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000378 0.000748 0.000896 0.001422 0.000340 0.000397 0.000668 0.001189 0.000358 0.000553 0.000741 0.001278
Bud Neck 0.003083 0.005774 0.012400 0.033694 0.003883 0.003738 0.015627 0.030213 0.003494 0.004643 0.014592 0.031542
Bud Site 0.000815 0.000655 0.001088 0.004626 0.000398 0.000380 0.000998 0.004875 0.000601 0.000502 0.001027 0.004780
Cell Periphery 0.000107 0.000087 0.000033 0.000157 0.000028 0.000026 0.000028 0.000152 0.000066 0.000053 0.000030 0.000154
Cytoplasm 0.004692 0.001087 0.000557 0.001551 0.000497 0.000697 0.000325 0.004138 0.002536 0.000870 0.000399 0.003150
Cytoplasmic Foci 0.000275 0.000423 0.000162 0.002224 0.000192 0.000348 0.000073 0.001773 0.000232 0.000382 0.000102 0.001946
Eisosomes 0.000027 0.000034 0.000046 0.000044 0.000030 0.000037 0.000058 0.000044 0.000028 0.000036 0.000054 0.000044
Endoplasmic Reticulum 0.001874 0.002026 0.001608 0.001840 0.001122 0.001174 0.000750 0.002355 0.001488 0.001553 0.001025 0.002158
Endosome 0.000187 0.000358 0.000086 0.004163 0.000053 0.000349 0.000160 0.002849 0.000118 0.000353 0.000136 0.003351
Golgi 0.000542 0.000491 0.000188 0.001923 0.000121 0.000501 0.000199 0.001992 0.000326 0.000496 0.000196 0.001966
Lipid Particles 0.000095 0.000443 0.000060 0.000597 0.000067 0.000553 0.000014 0.000388 0.000080 0.000504 0.000029 0.000467
Mitochondria 0.000723 0.001309 0.001374 0.006977 0.000875 0.001341 0.001470 0.004282 0.000801 0.001327 0.001440 0.005312
Mitotic Spindle 0.000419 0.002553 0.003909 0.037023 0.002465 0.002113 0.003692 0.021259 0.001471 0.002309 0.003761 0.027280
None 0.014291 0.007215 0.002336 0.004401 0.001170 0.001173 0.000838 0.006255 0.007547 0.003860 0.001319 0.005547
Nuclear Periphery 0.000151 0.000175 0.000165 0.002007 0.000137 0.000374 0.000295 0.001674 0.000144 0.000285 0.000254 0.001801
Nuclear Periphery Foci 0.002280 0.001426 0.000090 0.001067 0.000530 0.001374 0.000121 0.001198 0.001380 0.001397 0.000111 0.001148
Nucleolus 0.207072 0.176225 0.147138 0.137211 0.201681 0.208810 0.159968 0.080502 0.204301 0.194318 0.155850 0.102165
Nucleus 0.761980* 0.796632* 0.824927* 0.747831* 0.785423* 0.774874* 0.812187* 0.825869* 0.774029* 0.784551* 0.816276* 0.796059*
Peroxisomes 0.000448 0.001500 0.002376 0.001849 0.000705 0.001086 0.001743 0.001378 0.000580 0.001270 0.001946 0.001558
Vacuole 0.000333 0.000518 0.000356 0.005292 0.000135 0.000317 0.000368 0.004467 0.000231 0.000406 0.000364 0.004782
Vacuole Periphery 0.000230 0.000321 0.000204 0.004100 0.000151 0.000339 0.000420 0.003149 0.000189 0.000331 0.000350 0.003512

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.93 -15.93 -17.39 -11.85 -8.49 -1.52 -13.80 -18.63 -18.32 -11.32 -7.61 -20.69 -25.49 -20.75 -14.44
Bud Neck -6.82 -16.50 -18.13 -16.66 -12.29 0.73 -20.72 -20.90 -21.04 -10.15 -4.71 -26.22 -27.73 -26.61 -15.44
Bud Site 1.37 -2.26 -7.30 -7.82 -6.79 0.41 -6.28 -9.43 -9.60 -7.72 1.43 -5.40 -11.72 -12.34 -10.28
Cell Periphery 1.27 5.83 -1.69 -2.64 -5.60 0.53 -0.58 -6.91 -7.48 -7.13 1.69 4.78 -5.39 -6.77 -9.05
Cytoplasm 3.89 4.58 2.23 -3.19 -4.84 -0.66 -0.13 -6.61 -5.20 -6.63 3.33 4.40 -1.33 -6.15 -8.08
Cytoplasmic Foci -0.49 1.53 -1.98 -1.61 -2.37 -0.78 1.18 -2.73 -1.96 -3.46 -0.90 2.08 -3.33 -2.53 -4.14
Eisosomes -4.81 -13.62 -12.73 -7.88 1.07 -4.61 -18.57 -12.92 -5.19 7.11 -6.63 -23.31 -18.37 -9.04 6.55
Endoplasmic Reticulum -1.00 1.25 -3.13 -2.26 -4.03 -0.38 2.99 -7.35 -6.81 -8.50 -0.64 4.65 -7.36 -6.69 -9.72
Endosome -1.49 3.46 -3.25 -2.94 -3.41 -2.16 -1.56 -4.75 -3.60 -4.37 -2.56 -0.33 -5.54 -4.63 -5.44
Golgi 0.31 1.90 -1.41 -1.63 -2.46 -1.21 -1.60 -3.68 -1.73 -3.34 -0.77 1.30 -3.44 -2.39 -4.14
Lipid Particles -1.25 1.43 -1.85 0.15 -2.10 -1.48 1.49 -2.56 0.79 -3.29 -1.92 2.31 -3.03 0.75 -3.71
Mitochondria -4.10 -9.12 -7.58 -6.64 -6.35 -1.16 -3.85 -7.95 -5.82 -6.53 -2.43 -7.08 -11.18 -8.98 -8.96
Mitotic Spindle -2.94 -2.95 -9.34 -8.66 -7.96 0.44 -1.59 -8.37 -8.58 -7.31 -1.40 -3.33 -12.68 -12.16 -10.78
None 4.40 8.86 6.75 2.26 -3.70 0.03 0.41 -6.51 -6.48 -6.81 4.71 9.06 2.90 -2.22 -7.92
Nuclear Periphery -0.81 -1.50 -8.04 -7.97 -7.86 -1.15 -2.25 -6.46 -3.96 -4.83 -1.24 -2.44 -9.95 -7.57 -8.17
Nuclear Periphery Foci 1.35 4.25 2.88 1.26 -4.43 -1.45 3.16 -1.71 0.63 -3.96 0.12 4.90 1.70 1.18 -5.33
Nucleolus 5.27 6.72 12.03 7.05 4.23 -1.27 6.53 22.88* 23.50* 14.44 2.55 9.39 25.58 22.18 13.62
Nucleus -5.55 -6.26 1.72 5.71 6.39 1.76 -2.80 -4.59 -5.92 -1.95 -2.62 -6.53 -2.91 -0.75 2.63
Peroxisomes -5.11 -9.42 -11.38 -1.23 4.08 -3.45 -8.54 -3.08 -0.64 4.23 -6.32 -13.35 -8.13 -1.04 5.68
Vacuole -0.67 -1.23 -5.48 -5.23 -5.40 -1.12 -1.75 -5.87 -5.62 -5.13 -1.14 -1.74 -8.00 -7.65 -7.36
Vacuole Periphery -1.32 0.15 -2.97 -2.83 -2.98 -2.69 -1.77 -3.34 -2.96 -2.75 -2.85 -1.60 -4.44 -4.08 -3.96
Standard name
Human Ortholog
Description RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (95%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Rpc17

Rpc17


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpc17-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available