Standard name
Human Ortholog
Description Protein of unknown function; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs); NCE102 has a paralog, FHN1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.4 0.31 0.6 0.55 0.45 0.46 0.42 0.37 0.44 0.41 0.43 0.4 0.57 0.47 0.41 0.41 0.41 0.25 0.52 0.49 0.57 0.4 0.47 0.35
Bud 0.07 0.07 0.15 0.17 0.2 0.22 0.13 0.15 0.18 0.17 0.14 0.13 0.07 0 0.05 0.05 0.06 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.49 0.5 0.38 0.52 0.44 0.37 0.35 0.23 0.38 0.25 0.32 0.3 0.49 0.59 0.57 0.14 0.12 0.13 0.36 0.37 0.27 0.39 0.36 0.5
Cytoplasm 0 0.07 0 0 0.06 0 0.06 0.08 0.1 0.09 0.08 0.12 0 0 0 0.06 0.08 0.19 0 0 0 0 0 0
Endoplasmic Reticulum 0.08 0.15 0.09 0.09 0.09 0.15 0.19 0.24 0.14 0.19 0.17 0.21 0.17 0.16 0.18 0.06 0.05 0.1 0.05 0 0.09 0 0 0.07
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.45 0.34 0.25 0.25 0.16 0.1 0.14 0.2 0.17 0.34 0.18 0.16 0.32 0.31 0.25 0.59 0.48 0.46 0 0 0 0.05 0.05 0
Mitochondria 0.09 0 0.18 0.07 0.19 0.26 0.17 0.15 0.19 0.1 0.26 0.21 0 0 0 0.18 0.12 0.1 0.05 0 0 0.08 0.06 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 57 33 76 113 126 105 137 102 113 157 126 133 93 111 99 64 60 28 102 88 87 49 125 96
Bud 10 7 19 35 56 50 44 42 45 66 41 43 12 4 11 8 8 6 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 69 53 48 107 124 83 113 64 96 96 95 99 80 139 137 22 18 15 71 66 42 47 96 138
Cytoplasm 5 7 2 5 18 10 20 22 26 34 25 40 3 4 4 10 12 21 0 1 0 0 0 0
Endoplasmic Reticulum 11 16 11 18 26 33 63 66 35 72 49 71 27 38 44 9 7 11 10 8 13 5 8 19
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0
Golgi 64 36 31 51 46 22 47 55 43 131 52 52 52 74 60 91 70 52 2 4 3 5 12 6
Mitochondria 13 1 23 14 55 58 57 42 49 38 78 70 2 3 0 28 18 11 8 8 3 10 17 13
Nucleus 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 0 0 0 4 2 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 1 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 1 1
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Vac/Vac Membrane 1 0 3 0 3 2 0 2 0 0 2 1 0 0 0 2 6 0 0 1 0 0 1 0
Unique Cell Count 142 106 126 205 283 227 326 277 255 386 296 333 162 235 239 155 145 113 198 180 154 123 266 280
Labelled Cell Count 230 153 214 343 456 363 487 397 408 596 470 511 269 373 355 234 199 145 198 180 154 123 266 280


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 50.9 62.1 45.4 39.8 33.0 31.8 33.0 35.2 35.5 38.3 40.0 42.5 44.5 49.2 58.7 78.8 81.3 72.3 48.6 50.9 44.0
Std Deviation (1e-4) 12.8 16.8 16.4 9.1 9.1 9.1 10.2 10.6 13.3 12.5 16.4 16.8 12.9 16.3 21.4 29.6 26.6 30.1 13.7 13.0 10.1
Intensity Change (Log2) – – – -0.19 -0.46 -0.51 -0.46 -0.37 -0.35 -0.25 -0.18 -0.09 -0.03 0.11 0.37 0.79 0.84 0.67 0.1 0.16 -0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.9 -2.9 -2.5 -3.5 -4.4 -2.9 -3.8 -3.3 -3.9 -0.5 -2.4 -3.4 -3.2 -3.1 -5.5
Bud 0.5 1.1 1.6 -0.4 0 0.6 0.5 -0.3 -0.6 -2.1 -5.0 -3.5 -2.8 -2.6 -2.5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2.5 1.1 -0.3 -0.7 -3.1 -0.1 -2.9 -1.2 -1.7 1.9 3.8 3.5 -4.6 -4.9 -4.3
Cytoplasm 0 2.1 0 2.0 2.5 3.0 2.8 2.6 3.5 0 0 0 2.0 2.5 4.4
Endoplasmic Reticulum 0 0.1 1.6 2.7 3.6 1.4 2.6 2.1 3.1 2.0 2.0 2.5 -0.9 -1.3 0.3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.1 -2.0 -3.8 -2.6 -1.1 -1.8 2.0 -1.7 -2.2 1.4 1.4 0.1 5.7 4.0 3.5
Mitochondria -3.2 0.3 1.6 -0.2 -0.8 0.2 -2.5 1.8 0.7 -5.1 -5.9 -6.8 0 -1.3 -1.9
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 154.6261 159.2665 145.7397 136.5169 147.9427 163.5819 71.9145 86.7217 80.2092 75.3096 70.6166 87.9755 123.2457 138.0413 121.7444 131.1991 118.9839 141.3632
Actin 0.0001 0.0001 0 0.0001 0 0 0.0159 0.0086 0.0219 0.0017 0.0482 0.0002 0.0061 0.0002 0.0004 0.0001 0 0
Bud 0 0 0 0 0 0 0.0003 0.0001 0.0003 0.0002 0.0003 0 0 0 0 0 0 0
Bud Neck 0.0002 0.0002 0.0004 0.0001 0.0001 0.0003 0.0016 0.0009 0.001 0.001 0.0003 0.0004 0.0002 0.0006 0.0008 0.0004 0.0003 0.0001
Bud Periphery 0 0 0.0001 0 0 0 0.0005 0.0001 0.0007 0.0002 0.0006 0.0003 0 0 0 0 0 0
Bud Site 0 0.0001 0 0 0 0 0.0094 0.0068 0.0037 0.0014 0.002 0 0.0001 0.0009 0.0002 0 0 0
Cell Periphery 0.0053 0.0048 0.0221 0.0017 0.0019 0.008 0.0037 0.0043 0.0056 0.0087 0.0013 0.0172 0.0016 0.0045 0.0029 0.0036 0.0157 0.0006
Cytoplasm 0 0 0 0 0 0 0.0133 0 0.0004 0.0002 0.0016 0.0007 0.0005 0 0 0 0 0
Cytoplasmic Foci 0 0 0 0 0 0 0.0253 0.0001 0.0036 0.0231 0.0248 0.003 0.0043 0 0 0 0 0
Eisosomes 0.9933 0.9945 0.9705 0.9971 0.9975 0.9907 0.847 0.9778 0.9267 0.9289 0.8309 0.9597 0.9656 0.9932 0.995 0.9953 0.983 0.9992
Endoplasmic Reticulum 0.0001 0 0.0019 0.0003 0.0001 0.0001 0.0015 0 0.0035 0.0014 0.0009 0.0004 0.0001 0 0 0.0002 0.0002 0
Endosome 0 0 0 0 0 0 0.0084 0.0001 0.0043 0.0001 0.0143 0.0137 0.0006 0 0 0 0 0
Golgi 0 0 0.0001 0.0003 0.0001 0.0001 0.0037 0.0004 0.0029 0.0004 0.0156 0.0015 0.0006 0 0 0.0001 0.0001 0
Lipid Particles 0.0001 0 0.0001 0 0 0 0.0077 0 0.0013 0.0047 0.0183 0.0002 0.0024 0 0 0 0.0001 0
Mitochondria 0.0002 0 0.0001 0 0 0.0001 0.023 0.0004 0.0172 0.0136 0.0098 0.0002 0.0026 0.0001 0.0002 0.0001 0.0001 0
None 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0211 0 0.0029 0.0047 0.0016 0.0001 0.0034 0.0001 0 0.0001 0.0001 0
Nuclear Periphery 0.0003 0.0001 0.0029 0.0003 0.0002 0.0004 0.0018 0.0001 0.0006 0.0006 0.0035 0.0003 0.0003 0.0001 0.0001 0.0002 0.0002 0
Nucleolus 0 0 0 0 0 0 0.0027 0 0.0006 0.0017 0.0002 0 0.0056 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0.0016 0 0.0001 0.0002 0.0002 0 0.0012 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0.0069 0.0001 0.0021 0.0057 0.0224 0.0001 0.002 0.0001 0.0001 0 0 0
Punctate Nuclear 0 0 0 0 0 0 0.0026 0 0.0001 0.0009 0.0025 0 0.0025 0 0 0 0 0
Vacuole 0.0003 0.0001 0.0014 0 0 0.0001 0.0015 0 0.0004 0.0002 0.0003 0.0017 0.0001 0.0001 0.0001 0 0.0002 0
Vacuole Periphery 0 0 0.0001 0 0 0 0.0007 0 0.0002 0.0001 0.0005 0.0002 0.0001 0 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 59.1864 179.5129 465.521 491.003 101.4035 128.8705 350.1142 550.6226 548.2529 224.1986
Translational Efficiency 1.1689 1.382 1.5343 0.9295 1.5207 0.939 1.5608 1.5573 1.7319 1.6188

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1797 1393 1848 1070 1348 1556 1267 1449 3145 2949 3115 2519

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2755.16 3833.01 6224.93 7420.22 2575.61 2825.28 6624.80 7078.37 2678.20 3301.29 6387.57 7223.58
Standard Deviation 612.15 847.77 1261.28 1913.35 647.43 754.51 1260.32 1728.84 633.78 944.97 1276.10 1817.39
Intensity Change Log 2 — 0.476342 1.175922 1.429326 — 0.133480 1.362963 1.458503 — 0.320808 1.269322 1.443497

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.037551 0.011357 0.008884 0.029970 0.034118 0.034886 0.008344 0.014203 0.036079 0.023772 0.008664 0.020901
Bud Neck 0.002237 0.002296 0.002406 0.004394 0.003003 0.003368 0.002182 0.002172 0.002565 0.002862 0.002315 0.003116
Bud Site 0.000698 0.000639 0.000168 0.004916 0.000455 0.002394 0.000722 0.004735 0.000594 0.001565 0.000394 0.004812
Cell Periphery 0.006712 0.024697 0.019696 0.053709 0.003099 0.020439 0.042948 0.192368* 0.005163 0.022450 0.029153 0.133469*
Cytoplasm 0.000112 0.000114 0.000001 0.000145 0.000317 0.000178 0.000033 0.000199 0.000200 0.000148 0.000014 0.000176
Cytoplasmic Foci 0.000610 0.000175 0.000034 0.002002 0.000451 0.000391 0.000091 0.001738 0.000542 0.000289 0.000057 0.001850
Eisosomes 0.940610* 0.950455* 0.953974* 0.838808* 0.942582* 0.918668* 0.931807* 0.765780* 0.941455* 0.933683* 0.944958* 0.796800*
Endoplasmic Reticulum 0.003841 0.003675 0.006195 0.022407 0.003092 0.005130 0.004718 0.004329 0.003520 0.004442 0.005594 0.012008
Endosome 0.000620 0.000686 0.000738 0.002172 0.000651 0.001182 0.000582 0.001791 0.000633 0.000948 0.000674 0.001953
Golgi 0.001853 0.002201 0.003164 0.019785 0.004729 0.004639 0.002862 0.002044 0.003086 0.003487 0.003042 0.009580
Lipid Particles 0.001463 0.000563 0.000820 0.010054 0.004744 0.005091 0.001509 0.001604 0.002869 0.002952 0.001100 0.005193
Mitochondria 0.001192 0.000864 0.000411 0.002022 0.000500 0.001414 0.000958 0.003342 0.000895 0.001154 0.000633 0.002781
Mitotic Spindle 0.000406 0.000541 0.000374 0.000595 0.000247 0.000468 0.000430 0.001248 0.000338 0.000503 0.000397 0.000971
None 0.000677 0.000250 0.000123 0.000215 0.000876 0.000394 0.000128 0.000150 0.000763 0.000326 0.000125 0.000178
Nuclear Periphery 0.000270 0.000631 0.001711 0.003233 0.000226 0.000449 0.001397 0.001033 0.000251 0.000535 0.001583 0.001967
Nuclear Periphery Foci 0.000088 0.000027 0.000010 0.000058 0.000098 0.000031 0.000009 0.000053 0.000093 0.000029 0.000009 0.000055
Nucleolus 0.000020 0.000018 0.000008 0.000046 0.000040 0.000021 0.000014 0.000045 0.000028 0.000019 0.000010 0.000045
Nucleus 0.000152 0.000152 0.000043 0.000239 0.000175 0.000146 0.000060 0.000181 0.000162 0.000149 0.000050 0.000206
Peroxisomes 0.000047 0.000028 0.000072 0.000118 0.000047 0.000041 0.000017 0.000102 0.000047 0.000035 0.000050 0.000109
Vacuole 0.000540 0.000550 0.001093 0.004733 0.000442 0.000509 0.001096 0.002423 0.000498 0.000528 0.001094 0.003404
Vacuole Periphery 0.000302 0.000083 0.000076 0.000378 0.000107 0.000161 0.000093 0.000459 0.000219 0.000124 0.000083 0.000424

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 7.24 8.49 1.60 -4.85 -5.83 -0.17 7.90 5.34 5.19 -2.35 4.22 11.51 5.10 0.97 -5.81
Bud Neck -0.04 -0.38 -3.97 -3.92 -3.80 -0.80 2.69 1.69 2.01 -0.17 -0.94 1.31 -1.72 -0.84 -2.57
Bud Site 0.38 2.95 -3.06 -3.13 -3.52 -2.72 -0.49 -3.55 -1.81 -3.13 -2.29 0.98 -4.64 -3.44 -4.84
Cell Periphery -7.77 -7.18 -10.83 -6.20 -7.65 -8.63 -10.70 -26.13 -23.06 -18.47 -11.17 -12.89 -26.92 -22.57 -20.75
Cytoplasm 0.18 1.76 -0.08 -0.26 -2.41 0.40 0.99 0.31 -0.21 -2.20 0.40 1.61 0.19 -0.34 -3.29
Cytoplasmic Foci 1.80 2.55 -1.47 -1.94 -2.09 0.35 3.21 -1.98 -2.04 -2.46 1.66 3.44 -2.34 -2.82 -3.22
Eisosomes -2.01 -2.99 11.57 13.21 14.09 3.73 1.75 19.70 16.37 18.23 1.77 -1.07 22.59 21.24 24.07
Endoplasmic Reticulum 0.17 -2.10 -7.84 -7.86 -6.71 -2.13 -1.62 -1.42 0.75 0.35 -1.27 -2.67 -7.36 -6.33 -5.24
Endosome -0.59 -1.12 -8.60 -8.44 -8.33 -2.87 0.75 -2.35 -1.21 -2.52 -2.86 -0.50 -4.54 -3.37 -4.47
Golgi -0.55 -2.36 -7.77 -7.54 -7.18 0.07 1.75 2.54 3.05 1.15 -0.64 0.09 -5.80 -5.47 -6.09
Lipid Particles 1.40 1.16 -5.53 -6.45 -6.41 -0.22 2.41 2.27 2.78 -0.11 -0.08 2.71 -2.41 -2.40 -5.19
Mitochondria 1.64 4.06 -1.57 -2.30 -3.19 -3.53 -1.37 -3.95 -2.56 -3.03 -1.41 1.50 -3.95 -3.40 -4.45
Mitotic Spindle -1.42 0.75 -2.05 -0.72 -2.92 -5.02 -3.78 -1.69 -1.32 -1.38 -3.19 -1.25 -1.84 -1.39 -1.70
None 2.72 3.61 2.89 0.52 -2.62 0.99 1.53 1.48 5.78 -1.22 1.96 2.86 2.59 4.30 -2.95
Nuclear Periphery -4.44 -7.98 -9.81 -8.39 -4.35 -5.57 -8.91 -8.52 -6.00 2.30 -6.43 -11.13 -12.16 -9.85 -2.09
Nuclear Periphery Foci 1.01 1.31 0.57 -1.27 -2.59 1.06 1.43 0.62 -0.88 -1.89 1.43 1.88 0.83 -1.42 -2.93
Nucleolus 0.73 1.62 -0.81 -1.45 -2.05 1.10 1.53 -0.43 -2.18 -2.88 1.28 2.23 -0.98 -2.52 -3.52
Nucleus 0.52 1.53 -0.13 -0.66 -1.71 0.34 1.46 -0.44 -1.08 -2.77 0.65 1.98 -0.21 -1.04 -2.87
Peroxisomes 2.15 -0.60 -1.99 -2.72 -1.09 0.43 1.85 -1.18 -1.43 -1.99 1.57 0.04 -2.03 -2.64 -1.80
Vacuole 0.24 -1.55 -5.08 -5.44 -4.71 -0.35 -3.16 -3.40 -3.36 -2.35 0.08 -2.81 -5.86 -6.19 -4.96
Vacuole Periphery 1.23 1.27 -0.36 -4.02 -4.19 -0.58 0.47 -3.14 -2.16 -3.38 0.86 1.32 -1.62 -3.62 -4.92
Standard name
Human Ortholog
Description Protein of unknown function; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs); NCE102 has a paralog, FHN1, that arose from the whole genome duplication
Localization
Cell Percentages actin (11%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Nce102

Nce102


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nce102-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available