Standard name | |
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Description | Cyclin (Bur2p)-dependent protein kinase; part of the BUR kinase complex which functions in transcriptional regulation; phosphorylates the carboxy-terminal domain (CTD) of Rpo21p and the C-terminal repeat domain of Spt5p; recruits Spt6p to the CTD at the onset of transcription; regulated by Cak1p; similar to metazoan CDK9 proteins |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 6 | 2 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 7 | 0 | 3 | 19 | 13 | 15 | 23 | 16 | 13 | 12 | 9 | 8 | 16 | 34 | 16 | 43 | 24 | 0 | 0 | 0 | 0 | 4 | 2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 7 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 3 | 6 | 0 | 1 | 0 | 0 | 3 | 1 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 12 | 2 | 1 | 0 | 1 | 10 | 24 | 14 | 38 | 47 | 68 | 53 | 1 | 0 | 1 | 9 | 6 | 8 | 2 | 2 | 0 | 0 | 1 | 2 |
Nucleus | 312 | 203 | 63 | 165 | 246 | 284 | 459 | 511 | 270 | 364 | 318 | 346 | 84 | 211 | 215 | 230 | 490 | 362 | 308 | 202 | 72 | 51 | 210 | 293 |
Nuclear Periphery | 6 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 3 | 0 | 4 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 12 | 1 | 3 | 1 | 0 | 12 | 23 | 43 | 22 | 38 | 52 | 51 | 3 | 2 | 6 | 6 | 6 | 9 | 1 | 0 | 0 | 0 | 6 | 6 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 4 | 1 | 2 | 1 | 1 | 0 | 3 | 3 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 4 | 2 |
Vac/Vac Membrane | 3 | 5 | 1 | 2 | 2 | 2 | 6 | 3 | 4 | 5 | 2 | 1 | 0 | 4 | 2 | 8 | 16 | 19 | 5 | 4 | 2 | 0 | 22 | 33 |
Unique Cell Count | 326 | 215 | 68 | 170 | 265 | 309 | 503 | 562 | 313 | 423 | 377 | 397 | 96 | 232 | 255 | 261 | 563 | 413 | 325 | 217 | 81 | 54 | 257 | 351 |
Labelled Cell Count | 350 | 220 | 69 | 171 | 270 | 324 | 535 | 603 | 354 | 481 | 457 | 475 | 96 | 239 | 265 | 272 | 580 | 432 | 325 | 217 | 81 | 54 | 257 | 351 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.9 | 8.5 | 7.7 | 8.1 | 8.4 | 6.9 | 6.9 | 7.0 | 6.2 | 6.2 | 5.8 | 5.8 | 8.4 | 8.9 | 8.6 | 10.8 | 11.2 | 9.6 | 6.9 | 8.3 | 8.6 |
Std Deviation (1e-4) | 4.8 | 1.3 | 1.3 | 1.3 | 1.4 | 1.2 | 1.5 | 1.6 | 1.7 | 1.5 | 1.5 | 1.4 | 1.9 | 1.8 | 1.9 | 2.4 | 2.6 | 2.3 | 0.7 | 1.4 | 1.3 |
Intensity Change (Log2) | – | – | – | 0.08 | 0.13 | -0.14 | -0.16 | -0.14 | -0.31 | -0.3 | -0.4 | -0.41 | 0.13 | 0.21 | 0.17 | 0.5 | 0.54 | 0.32 | -0.16 | 0.11 | 0.17 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 2.6 | 2.5 | 3.5 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 1.5 | 0.1 | -0.2 | -0.4 | -0.5 | -1.4 | -1.5 | -1.8 | -1.3 | -1.1 | -0.4 | -1.8 | -1.1 | -1.3 | -1.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1.3 | 2.2 | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |