Standard name | |
---|---|
Human Ortholog | |
Description | Second largest subunit of DNA polymerase II (DNA polymerase epsilon); required for maintenance of fidelity of chromosomal replication; essential motif in C-terminus is required for formation of the four-subunit Pol epsilon; expression peaks at the G1/S phase boundary; Cdc28p substrate |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 2 | 2 | 1 | 0 | 0 | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 1 | 1 | 0 | 1 | 0 | 0 | 4 | 5 | 8 | – | 14 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 6 | 13 | 7 | 6 | 9 | 18 |
Bud Neck | 0 | 0 | 0 | 2 | 5 | 1 | 0 | 1 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 3 | 1 | 2 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 1 | 5 | 0 | 6 | – | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 82 | 74 | 137 | 129 | 86 | 66 | 76 | 90 | 47 | – | 96 | 63 | 59 | 144 | 202 | 137 | 140 | 153 | 74 | 67 | 147 | 49 | 105 | 182 |
Endoplasmic Reticulum | 3 | 1 | 11 | 2 | 1 | 0 | 2 | 1 | 0 | – | 0 | 0 | 0 | 1 | 7 | 9 | 7 | 11 | 0 | 0 | 2 | 4 | 2 | 8 |
Endosome | 7 | 7 | 0 | 1 | 2 | 4 | 8 | 15 | 1 | – | 1 | 13 | 37 | 110 | 42 | 1 | 1 | 2 | 15 | 12 | 5 | 1 | 4 | 5 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 1 | 0 | 0 | 0 | 3 | 1 |
Mitochondria | 8 | 7 | 0 | 2 | 2 | 37 | 103 | 18 | 193 | – | 283 | 140 | 8 | 2 | 2 | 4 | 1 | 1 | 0 | 1 | 7 | 3 | 8 | 16 |
Nucleus | 22 | 35 | 107 | 140 | 315 | 492 | 683 | 498 | 351 | – | 445 | 451 | 78 | 42 | 57 | 53 | 32 | 49 | 70 | 115 | 107 | 43 | 42 | 51 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | – | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 0 | 0 | 2 | 2 |
Nucleolus | 1 | 1 | 0 | 0 | 0 | 1 | 12 | 6 | 5 | – | 6 | 9 | 2 | 12 | 4 | 0 | 0 | 0 | 3 | 4 | 2 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 3 | 5 | 2 | 8 | 10 | 4 | – | 5 | 3 | 0 | 2 | 0 | 1 | 0 | 0 | 19 | 17 | 30 | 2 | 3 | 3 |
Vac/Vac Membrane | 78 | 120 | 46 | 59 | 145 | 110 | 123 | 103 | 35 | – | 87 | 120 | 207 | 300 | 244 | 103 | 106 | 131 | 28 | 31 | 68 | 69 | 117 | 146 |
Unique Cell Count | 178 | 210 | 271 | 313 | 517 | 653 | 902 | 683 | 507 | 738 | 695 | 355 | 502 | 500 | 256 | 246 | 286 | 230 | 273 | 391 | 185 | 304 | 448 | |
Labelled Cell Count | 203 | 246 | 306 | 342 | 563 | 714 | 1024 | 750 | 653 | 939 | 807 | 391 | 614 | 558 | 313 | 290 | 353 | 230 | 273 | 391 | 185 | 304 | 448 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.4 | 6.0 | 6.5 | 5.9 | 6.5 | 5.5 | 5.6 | 6.1 | 4.6 | – | 4.7 | 5.3 | 6.6 | 6.8 | 6.8 | 12.7 | 12.7 | 13.0 | 5.7 | 5.5 | 6.0 |
Std Deviation (1e-4) | 1.1 | 1.1 | 0.7 | 0.7 | 0.9 | 0.7 | 0.9 | 0.9 | 0.8 | – | 0.8 | 0.9 | 1.3 | 1.4 | 1.2 | 2.4 | 2.6 | 2.4 | 1.0 | 0.9 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.15 | 0.0 | -0.25 | -0.2 | -0.09 | -0.49 | – | -0.46 | -0.29 | 0.01 | 0.07 | 0.06 | 0.96 | 0.97 | 1.0 | -0.18 | -0.23 | -0.12 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -2.3 | -10.0 | -13.5 | -15.8 | -12.2 | -12.9 | – | -12.5 | -14.3 | -9.1 | -6.0 | -2.7 | 0.7 | 1.4 | 0.7 |
Endoplasmic Reticulum | -2.8 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | -2.3 | -0.3 | -0.8 | -0.1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 5.5 | 8.3 | 4.9 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.0 | 5.8 | 2.7 | 0 | – | 0 | 8.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 1.3 | 5.7 | 10.4 | 11.2 | 9.7 | 8.0 | – | 5.9 | 7.2 | -4.8 | -10.5 | -9.1 | -4.7 | -6.8 | -5.9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.6 | 3.4 | 0 | -1.4 | -0.7 | -4.4 | – | -2.2 | 0.1 | 10.4 | 11.4 | 8.7 | 5.9 | 6.5 | 7.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.8579 | 2.465 | 2.2101 | 1.7706 | 1.1333 | 1.6417 | 1.6697 | 2.7534 | 2.781 | 1.9791 | 1.8979 | 2.0582 | 2.791 | 4.4729 | 3.8739 | 3.941 | 2.9641 | 3.5243 |
Actin | 0.0088 | 0.0002 | 0.0026 | 0.0002 | 0.0002 | 0.0042 | 0.0224 | 0.0007 | 0.0087 | 0.0126 | 0.0021 | 0.0044 | 0.0443 | 0.0008 | 0.0012 | 0 | 0.0001 | 0.0259 |
Bud | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.001 | 0.0002 | 0.0024 | 0.0004 | 0.0001 | 0.0005 | 0.0009 | 0.0031 | 0.0006 | 0 | 0 | 0.0005 |
Bud Neck | 0.0011 | 0.0005 | 0.0028 | 0.0005 | 0.0006 | 0.0025 | 0.0014 | 0.0005 | 0.0014 | 0.0006 | 0.0007 | 0.0031 | 0.0012 | 0.0006 | 0.0017 | 0.0002 | 0.0006 | 0.0024 |
Bud Periphery | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0.0012 | 0.0002 | 0.0013 | 0.0005 | 0.0001 | 0.0007 | 0.0009 | 0.0012 | 0.0004 | 0 | 0 | 0.0006 |
Bud Site | 0.0006 | 0.0007 | 0.0063 | 0.0001 | 0.0001 | 0.0001 | 0.0069 | 0.0016 | 0.0069 | 0.0013 | 0.0001 | 0.0009 | 0.0052 | 0.0034 | 0.0092 | 0.0001 | 0.0001 | 0.0003 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.0552 | 0.0793 | 0.0507 | 0.0832 | 0.0112 | 0.0341 | 0.0733 | 0.1524 | 0.1172 | 0.0764 | 0.0666 | 0.0603 | 0.1157 | 0.1739 | 0.1124 | 0.0554 | 0.0248 | 0.0736 |
Cytoplasmic Foci | 0.0049 | 0.001 | 0.0056 | 0.0008 | 0.0011 | 0.0013 | 0.0358 | 0.0024 | 0.0043 | 0.0089 | 0.0078 | 0.0121 | 0.0459 | 0.0091 | 0.0098 | 0.0005 | 0.0001 | 0.0052 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0049 | 0.0048 | 0.001 | 0.0073 | 0.0003 | 0.0016 | 0.0051 | 0.0038 | 0.0047 | 0.0047 | 0.0015 | 0.0023 | 0.0068 | 0.0015 | 0.0021 | 0.0004 | 0.0003 | 0.005 |
Endosome | 0.0106 | 0.0018 | 0.0035 | 0.0039 | 0.0002 | 0.0026 | 0.0612 | 0.0055 | 0.0077 | 0.0398 | 0.0065 | 0.022 | 0.0294 | 0.0022 | 0.0151 | 0.0002 | 0.0001 | 0.0159 |
Golgi | 0.0017 | 0 | 0.0003 | 0 | 0 | 0.0002 | 0.014 | 0.0025 | 0.0026 | 0.0099 | 0.0057 | 0.0085 | 0.0264 | 0.0003 | 0.0008 | 0 | 0 | 0.0078 |
Lipid Particles | 0.0023 | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0003 | 0.0136 | 0.0003 | 0.0006 | 0.0033 | 0.03 | 0.0089 | 0.0247 | 0.0004 | 0.0004 | 0 | 0 | 0.0007 |
Mitochondria | 0.0009 | 0.0002 | 0.0033 | 0.0002 | 0.0001 | 0.0004 | 0.0123 | 0.0097 | 0.0072 | 0.0258 | 0.0008 | 0.0162 | 0.011 | 0.0022 | 0.0005 | 0.0002 | 0.0001 | 0.0072 |
None | 0.0304 | 0.0139 | 0.0013 | 0.036 | 0.0004 | 0.015 | 0.0126 | 0.005 | 0.0053 | 0.0017 | 0.0004 | 0.0011 | 0.0519 | 0.004 | 0.0008 | 0.0003 | 0.0985 | 0.0005 |
Nuclear Periphery | 0.0293 | 0.0149 | 0.0146 | 0.0175 | 0.007 | 0.012 | 0.0243 | 0.0221 | 0.0311 | 0.0231 | 0.016 | 0.0144 | 0.0191 | 0.0151 | 0.0177 | 0.0091 | 0.0069 | 0.0131 |
Nucleolus | 0.0037 | 0.0031 | 0.0045 | 0.0013 | 0.0031 | 0.0056 | 0.0065 | 0.0023 | 0.0188 | 0.0011 | 0.0009 | 0.0053 | 0.0025 | 0.0024 | 0.0043 | 0.0008 | 0.0013 | 0.0025 |
Nucleus | 0.8189 | 0.8681 | 0.8812 | 0.8445 | 0.9384 | 0.8982 | 0.6635 | 0.7673 | 0.7588 | 0.7773 | 0.8431 | 0.8095 | 0.584 | 0.7693 | 0.7765 | 0.8969 | 0.865 | 0.8248 |
Peroxisomes | 0.0021 | 0 | 0.0004 | 0 | 0 | 0.0001 | 0.0088 | 0.0003 | 0.0004 | 0.0028 | 0.0118 | 0.0078 | 0.0146 | 0.0003 | 0.0006 | 0 | 0 | 0.0071 |
Punctate Nuclear | 0.0205 | 0.0097 | 0.0196 | 0.0025 | 0.0369 | 0.0204 | 0.0245 | 0.0211 | 0.0152 | 0.0038 | 0.0042 | 0.0169 | 0.01 | 0.009 | 0.0419 | 0.0354 | 0.0017 | 0.0051 |
Vacuole | 0.0028 | 0.0011 | 0.0011 | 0.0013 | 0.0002 | 0.0009 | 0.0082 | 0.0017 | 0.004 | 0.0035 | 0.0013 | 0.0037 | 0.0039 | 0.001 | 0.0034 | 0.0003 | 0.0002 | 0.001 |
Vacuole Periphery | 0.0007 | 0.0002 | 0.0005 | 0.0003 | 0.0001 | 0.0002 | 0.0029 | 0.0004 | 0.0012 | 0.002 | 0.0003 | 0.0011 | 0.0013 | 0.0003 | 0.0005 | 0.0001 | 0.0001 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 38.1128 | 10.0067 | 4.2431 | 18.4853 | 25.5713 | 28.2358 | 10.9609 | 7.3166 | 12.3063 | 28.1693 |
Translational Efficiency | 0.8467 | 0.6667 | 1.2351 | 0.559 | 0.7571 | 1.1806 | 0.6586 | 0.7035 | 0.7533 | 0.7508 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2325 | 671 | 1160 | 355 | 1624 | 392 | 1518 | 696 | 3949 | 1063 | 2678 | 1051 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 754.76 | 827.99 | 1023.71 | 1127.40 | 765.74 | 869.08 | 915.33 | 1067.35 | 759.28 | 843.14 | 962.28 | 1087.63 |
Standard Deviation | 87.62 | 165.80 | 157.80 | 161.11 | 94.43 | 114.48 | 130.96 | 164.45 | 90.64 | 150.26 | 152.94 | 165.78 |
Intensity Change Log 2 | — | 0.133595 | 0.439717 | 0.578910 | — | 0.182634 | 0.257437 | 0.479107 | — | 0.158500 | 0.350797 | 0.529511 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000068 | 0.000667 | 0.000719 | 0.001744 | 0.000172 | 0.000610 | 0.000271 | 0.001135 | 0.000111 | 0.000646 | 0.000465 | 0.001341 |
Bud Neck | 0.004640 | 0.004176 | 0.008056 | 0.013890 | 0.003380 | 0.006705 | 0.008927 | 0.015159 | 0.004122 | 0.005109 | 0.008550 | 0.014731 |
Bud Site | 0.001804 | 0.003087 | 0.002618 | 0.044164 | 0.001638 | 0.008132 | 0.007888 | 0.009926 | 0.001736 | 0.004947 | 0.005605 | 0.021491 |
Cell Periphery | 0.000104 | 0.000161 | 0.000148 | 0.000380 | 0.000107 | 0.000215 | 0.000368 | 0.000142 | 0.000105 | 0.000181 | 0.000273 | 0.000222 |
Cytoplasm | 0.510846* | 0.276076* | 0.064402 | 0.171131* | 0.274678* | 0.227004* | 0.292876* | 0.211305* | 0.413723* | 0.257980* | 0.193911* | 0.197735* |
Cytoplasmic Foci | 0.045260 | 0.019787 | 0.000487 | 0.022049 | 0.025760 | 0.058983 | 0.015306 | 0.005539 | 0.037241 | 0.034241 | 0.008887 | 0.011116 |
Eisosomes | 0.000027 | 0.000062 | 0.000016 | 0.000063 | 0.000030 | 0.000080 | 0.000063 | 0.000021 | 0.000028 | 0.000069 | 0.000043 | 0.000035 |
Endoplasmic Reticulum | 0.001108 | 0.005589 | 0.005681 | 0.015004 | 0.002839 | 0.004391 | 0.002227 | 0.003876 | 0.001820 | 0.005147 | 0.003723 | 0.007635 |
Endosome | 0.002798 | 0.002566 | 0.000499 | 0.057703 | 0.001421 | 0.006617 | 0.002844 | 0.003437 | 0.002231 | 0.004060 | 0.001829 | 0.021766 |
Golgi | 0.000416 | 0.000689 | 0.000084 | 0.027318 | 0.000346 | 0.004003 | 0.001261 | 0.001218 | 0.000387 | 0.001911 | 0.000751 | 0.010034 |
Lipid Particles | 0.002191 | 0.002281 | 0.000336 | 0.002816 | 0.001785 | 0.006269 | 0.003134 | 0.001222 | 0.002024 | 0.003751 | 0.001922 | 0.001761 |
Mitochondria | 0.001463 | 0.005031 | 0.001366 | 0.002818 | 0.001844 | 0.006846 | 0.001605 | 0.001378 | 0.001620 | 0.005700 | 0.001502 | 0.001864 |
Mitotic Spindle | 0.003350 | 0.004749 | 0.005899 | 0.109505* | 0.003636 | 0.010786 | 0.010284 | 0.027159 | 0.003468 | 0.006975 | 0.008384 | 0.054974 |
None | 0.020003 | 0.014363 | 0.004209 | 0.012226 | 0.009410 | 0.008919 | 0.019979 | 0.017640 | 0.015647 | 0.012356 | 0.013148 | 0.015812 |
Nuclear Periphery | 0.001041 | 0.001518 | 0.001384 | 0.003200 | 0.001841 | 0.001450 | 0.001376 | 0.001458 | 0.001370 | 0.001493 | 0.001379 | 0.002046 |
Nuclear Periphery Foci | 0.003426 | 0.004880 | 0.000697 | 0.012280 | 0.004310 | 0.003373 | 0.000994 | 0.001215 | 0.003790 | 0.004325 | 0.000865 | 0.004952 |
Nucleolus | 0.005639 | 0.005609 | 0.000611 | 0.002021 | 0.005155 | 0.005179 | 0.001877 | 0.000715 | 0.005440 | 0.005450 | 0.001328 | 0.001156 |
Nucleus | 0.385633* | 0.628505* | 0.892678* | 0.399821* | 0.652758* | 0.595020* | 0.596490* | 0.669781* | 0.495487* | 0.616157* | 0.724786* | 0.578596* |
Peroxisomes | 0.001066 | 0.002297 | 0.000100 | 0.008211 | 0.000787 | 0.005027 | 0.001948 | 0.000383 | 0.000951 | 0.003304 | 0.001147 | 0.003027 |
Vacuole | 0.008848 | 0.017616 | 0.009864 | 0.082510 | 0.007853 | 0.039143 | 0.028892 | 0.026886 | 0.008438 | 0.025555 | 0.020650 | 0.045674 |
Vacuole Periphery | 0.000270 | 0.000291 | 0.000145 | 0.011143 | 0.000251 | 0.001248 | 0.001392 | 0.000405 | 0.000262 | 0.000644 | 0.000852 | 0.004032 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -8.90 | -12.82 | -10.61 | -3.97 | -3.33 | -3.62 | -6.72 | -7.83 | -2.76 | -4.10 | -9.18 | -14.77 | -13.01 | -4.00 | -4.81 |
Bud Neck | -0.23 | -7.18 | -5.73 | -5.61 | -3.08 | -2.62 | -6.04 | -5.89 | -3.68 | -1.63 | -1.92 | -8.59 | -7.94 | -6.81 | -3.09 |
Bud Site | -3.63 | -3.35 | -5.43 | -4.37 | -4.75 | -2.90 | -4.40 | -5.26 | -0.04 | -0.95 | -4.23 | -5.41 | -7.69 | -3.56 | -4.19 |
Cell Periphery | -3.49 | -2.56 | -6.15 | -3.50 | -3.60 | -3.74 | -6.76 | -3.84 | 2.28 | 4.96 | -5.13 | -6.62 | -6.42 | 0.04 | 1.89 |
Cytoplasm | 20.83 | 70.35* | 38.31 | 11.01 | -7.42 | 3.34 | 5.05 | 12.12 | 5.28 | 7.55 | 17.63 | 41.77 | 36.10 | 11.28 | 1.59 |
Cytoplasmic Foci | 8.61 | 23.99 | 20.04 | 5.89 | -4.76 | -5.56 | 8.91 | 13.29 | 10.52 | 6.21 | 1.67 | 22.71 | 24.37 | 11.85 | 3.43 |
Eisosomes | -2.57 | 4.08 | -9.51 | -0.31 | -11.10 | -7.94 | -8.79 | -0.25 | 7.85 | 8.62 | -4.47 | -7.32 | -7.24 | 2.75 | -0.13 |
Endoplasmic Reticulum | -10.57 | -15.59 | -9.05 | -3.18 | -2.87 | -3.01 | -6.46 | -5.48 | -0.39 | 0.21 | -10.05 | -16.84 | -11.93 | -1.68 | -1.75 |
Endosome | 0.20 | 5.16 | -2.61 | -2.63 | -3.74 | -5.46 | -2.14 | 0.82 | 5.49 | 2.29 | -3.11 | 2.22 | -1.77 | 0.35 | -2.75 |
Golgi | -0.80 | 5.29 | -1.80 | -1.65 | -2.01 | -1.94 | -1.02 | -0.04 | 1.92 | 0.88 | -2.08 | -0.15 | -1.72 | 0.39 | -1.60 |
Lipid Particles | -0.79 | 8.68 | -0.04 | 0.75 | -7.97 | -3.90 | -0.84 | 4.13 | 4.74 | 2.47 | -3.66 | 1.66 | 3.57 | 5.03 | 0.36 |
Mitochondria | -1.43 | -0.67 | -2.27 | 1.07 | -2.96 | -1.64 | -0.11 | -0.04 | 1.66 | 0.13 | -2.13 | -0.60 | -1.19 | 1.93 | -0.96 |
Mitotic Spindle | -1.44 | -2.14 | -6.57 | -6.04 | -5.87 | -1.60 | -5.66 | -5.14 | -2.79 | -1.37 | -2.14 | -6.12 | -8.31 | -6.61 | -5.21 |
None | 3.33 | 14.40 | 4.66 | 0.76 | -5.12 | 0.72 | -2.16 | -3.47 | -2.82 | -0.43 | 3.00 | 5.90 | 2.34 | -1.13 | -3.26 |
Nuclear Periphery | -2.98 | -4.66 | -8.41 | -7.03 | -7.05 | 0.97 | -4.24 | -3.75 | -4.93 | -0.78 | -1.62 | -6.99 | -8.73 | -7.89 | -5.12 |
Nuclear Periphery Foci | -2.06 | 5.07 | 0.03 | 1.83 | -2.83 | 0.89 | 3.07 | 4.22 | 1.31 | 1.26 | -1.10 | 4.75 | 3.34 | 2.91 | -1.26 |
Nucleolus | -0.09 | 8.87 | 7.60 | 3.67 | -1.84 | 2.27 | 4.84 | 6.27 | 3.58 | 2.73 | 0.87 | 8.97 | 10.04 | 4.71 | 1.63 |
Nucleus | -18.18 | -68.50 | -8.95 | 4.30 | 21.29 | 3.56 | 1.36 | -3.57 | -5.59 | -4.55 | -10.70 | -34.57 | -14.08 | -2.46 | 9.68 |
Peroxisomes | -1.38 | 3.82 | -0.26 | 0.95 | -1.90 | -3.54 | -0.91 | 4.26 | 4.24 | 3.10 | -3.36 | 0.98 | 1.81 | 4.04 | 0.79 |
Vacuole | -6.13 | -4.46 | -15.54 | -13.67 | -14.91 | -7.42 | -15.18 | -11.81 | -3.07 | -2.71 | -9.55 | -15.48 | -18.53 | -12.52 | -12.55 |
Vacuole Periphery | -0.59 | 2.85 | -2.34 | -2.30 | -2.53 | -2.94 | -1.57 | 0.65 | 3.05 | 1.67 | -2.98 | -1.36 | -2.13 | -0.22 | -0.39 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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