Standard name | |
---|---|
Human Ortholog | |
Description | Ski complex component and TPR protein; mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay; mutations in the human ortholog, TTC37, causes Syndromic diarrhea/Trichohepatoenteric (SD/THE) syndrome |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 4 | 1 | 3 | 3 | 0 | 2 | 1 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 0 | 4 | 2 | 4 | 4 | 8 | 7 | 2 | 9 | 7 | 5 | 2 | 0 | 6 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 0 | 2 | 4 | 11 | 13 | 18 | 24 | 13 | 25 | 13 | 11 | 5 | 6 | 4 | 47 | 19 | 32 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 240 | 62 | 134 | 183 | 286 | 263 | 365 | 382 | 167 | 229 | 111 | 154 | 185 | 263 | 297 | 766 | 347 | 678 | 273 | 70 | 136 | 69 | 287 | 284 |
Endoplasmic Reticulum | 4 | 0 | 1 | 1 | 6 | 7 | 22 | 6 | 7 | 10 | 11 | 5 | 1 | 1 | 4 | 30 | 21 | 25 | 8 | 1 | 2 | 1 | 11 | 30 |
Endosome | 1 | 0 | 0 | 0 | 2 | 0 | 2 | 5 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 0 | 0 | 0 | 0 | 1 | 0 |
Mitochondria | 15 | 5 | 16 | 30 | 13 | 72 | 158 | 206 | 122 | 195 | 110 | 146 | 1 | 2 | 15 | 15 | 7 | 10 | 4 | 1 | 5 | 0 | 1 | 1 |
Nucleus | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 1 | 0 | 2 | 0 | 2 | 0 | 0 | 8 | 6 | 12 | 1 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 3 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 18 | 0 | 1 | 1 | 6 | 0 | 14 | 57 | 4 | 18 | 4 | 17 | 0 | 1 | 1 | 32 | 8 | 55 | 2 | 1 | 5 | 1 | 8 | 4 |
Unique Cell Count | 271 | 63 | 143 | 191 | 301 | 305 | 487 | 562 | 254 | 381 | 197 | 262 | 190 | 267 | 305 | 818 | 369 | 750 | 294 | 76 | 153 | 74 | 312 | 327 |
Labelled Cell Count | 287 | 67 | 158 | 221 | 331 | 362 | 590 | 695 | 323 | 491 | 262 | 340 | 198 | 274 | 328 | 903 | 410 | 818 | 294 | 76 | 153 | 74 | 312 | 327 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.9 | 9.7 | 6.8 | 7.1 | 7.4 | 6.9 | 6.6 | 6.6 | 5.9 | 6.1 | 5.6 | 5.4 | 11.0 | 10.3 | 9.1 | 12.1 | 12.7 | 13.8 | 10.2 | 11.0 | 11.9 |
Std Deviation (1e-4) | 2.0 | 2.0 | 1.1 | 1.5 | 1.3 | 1.3 | 1.2 | 1.4 | 1.2 | 1.4 | 1.3 | 1.2 | 2.0 | 1.5 | 1.4 | 2.2 | 2.4 | 2.6 | 1.4 | 2.0 | 2.2 |
Intensity Change (Log2) | – | – | – | 0.06 | 0.13 | 0.03 | -0.05 | -0.04 | -0.21 | -0.16 | -0.27 | -0.32 | 0.69 | 0.6 | 0.43 | 0.84 | 0.9 | 1.02 | 0.58 | 0.7 | 0.81 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 1.6 | 1.9 | 2.4 | 2.3 | 0 | 0 | 0 | 0 | 2.2 | 1.9 | 1.6 |
Cytoplasm | 0.9 | 0.6 | -2.3 | -4.9 | -6.2 | -6.2 | -7.4 | -7.6 | -7.4 | 1.6 | 2.6 | 1.9 | 0 | 0.1 | -1.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 2.1 | 0 | 0 | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 2.5 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.2 | -2.7 | 3.1 | 5.0 | 5.9 | 7.4 | 8.3 | 8.4 | 8.7 | -4.4 | -4.9 | -2.4 | 0 | -4.6 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 3.7 | 0 | 2.2 | 0 | 2.7 | 0 | 0 | 0 | 0 | 0 | 3.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.9818 | 8.6448 | 6.5878 | 5.9502 | 7.3369 | 7.6543 | 7.5928 | 11.7008 | 10.8959 | 10.4194 | 9.7838 | 10.4821 | 1.8078 | 6.2907 | 5.6112 | 6.4179 | 5.4253 | 5.9462 |
Actin | 0.0503 | 0.0186 | 0.0231 | 0.0024 | 0.0001 | 0.0004 | 0.0104 | 0.0026 | 0.004 | 0.0003 | 0.0003 | 0.0008 | 0.0335 | 0.0015 | 0.0012 | 0.0001 | 0.0002 | 0.0017 |
Bud | 0.0011 | 0.0006 | 0.0024 | 0.0011 | 0.0004 | 0.0002 | 0.0008 | 0.0003 | 0.0005 | 0.0011 | 0.0008 | 0.0016 | 0.0005 | 0.0007 | 0.0011 | 0.0008 | 0.0002 | 0.0005 |
Bud Neck | 0.0028 | 0.0005 | 0.0014 | 0.0008 | 0.0004 | 0.0016 | 0.0007 | 0.0006 | 0.0013 | 0.0008 | 0.0007 | 0.001 | 0.0006 | 0.0002 | 0.0003 | 0.0004 | 0.0003 | 0.0023 |
Bud Periphery | 0.0021 | 0.0004 | 0.0031 | 0.0026 | 0 | 0.0001 | 0.0006 | 0.0002 | 0.0003 | 0.0003 | 0.0005 | 0.0012 | 0.0005 | 0.0005 | 0.0002 | 0.0001 | 0 | 0.0003 |
Bud Site | 0.0209 | 0.0019 | 0.0037 | 0.0007 | 0.0001 | 0.0001 | 0.0063 | 0.0026 | 0.0015 | 0.0003 | 0.0007 | 0.0003 | 0.0013 | 0.0014 | 0.0009 | 0.0002 | 0.0001 | 0.0001 |
Cell Periphery | 0.0051 | 0.0006 | 0.0015 | 0.0005 | 0 | 0.0001 | 0.0005 | 0.0005 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0.0008 | 0.0002 | 0 | 0 | 0.0002 |
Cytoplasm | 0.5709 | 0.8241 | 0.8109 | 0.5887 | 0.9656 | 0.8104 | 0.7732 | 0.9523 | 0.9031 | 0.9189 | 0.7987 | 0.893 | 0.3317 | 0.8931 | 0.933 | 0.9225 | 0.9615 | 0.8414 |
Cytoplasmic Foci | 0.0252 | 0.005 | 0.0118 | 0.0155 | 0.0071 | 0.0071 | 0.0192 | 0.0058 | 0.0138 | 0.01 | 0.0116 | 0.012 | 0.0581 | 0.0158 | 0.0178 | 0.0165 | 0.0096 | 0.0205 |
Eisosomes | 0.0004 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.1094 | 0.029 | 0.0316 | 0.0093 | 0.0118 | 0.133 | 0.0485 | 0.0049 | 0.0127 | 0.0069 | 0.0167 | 0.027 | 0.0144 | 0.0103 | 0.0043 | 0.0015 | 0.0039 | 0.0436 |
Endosome | 0.0542 | 0.0154 | 0.0366 | 0.1372 | 0.0101 | 0.0313 | 0.0659 | 0.0089 | 0.0314 | 0.0336 | 0.0623 | 0.0346 | 0.1156 | 0.0212 | 0.0312 | 0.0305 | 0.0169 | 0.0588 |
Golgi | 0.0148 | 0.0036 | 0.0101 | 0.0415 | 0.0009 | 0.0028 | 0.0075 | 0.0013 | 0.0073 | 0.0011 | 0.002 | 0.0032 | 0.0133 | 0.0048 | 0.0019 | 0.0005 | 0.0006 | 0.0116 |
Lipid Particles | 0.0114 | 0.0003 | 0.0032 | 0.0639 | 0 | 0 | 0.0093 | 0.0009 | 0.0046 | 0 | 0.0002 | 0.0002 | 0.2142 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Mitochondria | 0.0627 | 0.0024 | 0.0105 | 0.0933 | 0.0008 | 0.0039 | 0.0064 | 0.0018 | 0.0022 | 0.001 | 0.0015 | 0.0132 | 0.0077 | 0.0047 | 0.0004 | 0.0002 | 0.0005 | 0.0118 |
None | 0.0061 | 0.0004 | 0.0012 | 0.001 | 0.0003 | 0.0006 | 0.0026 | 0.0009 | 0.0008 | 0.0014 | 0.0006 | 0.0008 | 0.0285 | 0.0401 | 0.001 | 0.0011 | 0.0016 | 0.0013 |
Nuclear Periphery | 0.0079 | 0.0014 | 0.004 | 0.0133 | 0.0002 | 0.0026 | 0.0077 | 0.0015 | 0.0018 | 0.0019 | 0.0064 | 0.0025 | 0.1101 | 0.0008 | 0.0006 | 0.0015 | 0.001 | 0.0009 |
Nucleolus | 0.0004 | 0.0048 | 0.0069 | 0.0003 | 0 | 0 | 0.0011 | 0.0025 | 0 | 0 | 0.0027 | 0 | 0.0053 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0027 | 0.085 | 0.0164 | 0.0015 | 0.0003 | 0.0012 | 0.0045 | 0.0042 | 0.0012 | 0.0019 | 0.0153 | 0.0016 | 0.0245 | 0.0006 | 0.0007 | 0.0019 | 0.0012 | 0.001 |
Peroxisomes | 0.0036 | 0.0001 | 0.0053 | 0.0009 | 0 | 0 | 0.0034 | 0.0001 | 0.0025 | 0.0001 | 0.0001 | 0.0001 | 0.0054 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.0045 | 0.0001 | 0.0004 | 0.0013 | 0 | 0.0001 | 0.0007 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0224 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 |
Vacuole | 0.0355 | 0.0048 | 0.0126 | 0.0143 | 0.001 | 0.0028 | 0.0244 | 0.0063 | 0.009 | 0.0169 | 0.0617 | 0.0039 | 0.0066 | 0.0026 | 0.0037 | 0.0185 | 0.0015 | 0.0024 |
Vacuole Periphery | 0.0079 | 0.0009 | 0.0031 | 0.01 | 0.0007 | 0.0018 | 0.0063 | 0.0013 | 0.0014 | 0.0035 | 0.0169 | 0.0027 | 0.0049 | 0.0007 | 0.0011 | 0.0035 | 0.0006 | 0.0014 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 26.6932 | 19.5027 | 26.9006 | 32.0061 | 25.1247 | 23.8567 | 27.1428 | 30.7675 | 29.3827 | 36.5702 |
Translational Efficiency | 1.2077 | 1.0967 | 0.8756 | 0.8056 | 1.1184 | 1.1627 | 0.9079 | 0.6335 | 0.7621 | 0.7753 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
658 | 1229 | 243 | 620 | 1358 | 1974 | 247 | 988 | 2016 | 3203 | 490 | 1608 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1009.01 | 1143.47 | 1667.53 | 1627.78 | 1084.92 | 1093.18 | 1451.55 | 1490.40 | 1060.14 | 1112.48 | 1558.66 | 1543.37 |
Standard Deviation | 204.42 | 188.90 | 223.10 | 264.82 | 154.90 | 174.89 | 214.09 | 216.33 | 176.26 | 182.05 | 243.83 | 245.49 |
Intensity Change Log 2 | — | 0.180478 | 0.724772 | 0.689965 | — | 0.010942 | 0.420006 | 0.458111 | — | 0.095126 | 0.574907 | 0.574491 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000829 | 0.000595 | 0.001513 | 0.000959 | 0.000388 | 0.000293 | 0.000507 | 0.000552 | 0.000532 | 0.000409 | 0.001006 | 0.000709 |
Bud Neck | 0.001426 | 0.000575 | 0.005319 | 0.002260 | 0.000479 | 0.000582 | 0.003521 | 0.001091 | 0.000789 | 0.000579 | 0.004413 | 0.001542 |
Bud Site | 0.094832 | 0.036119 | 0.034506 | 0.032686 | 0.022372 | 0.023735 | 0.015733 | 0.027559 | 0.046022 | 0.028487 | 0.025043 | 0.029536 |
Cell Periphery | 0.001962 | 0.000422 | 0.001626 | 0.001673 | 0.000238 | 0.000198 | 0.000163 | 0.001500 | 0.000801 | 0.000284 | 0.000888 | 0.001567 |
Cytoplasm | 0.536646* | 0.727962* | 0.737301* | 0.818615* | 0.758908* | 0.771372* | 0.546400* | 0.838152* | 0.686364* | 0.754716* | 0.641072* | 0.830619* |
Cytoplasmic Foci | 0.203608* | 0.159706 | 0.052431 | 0.036738 | 0.151251 | 0.133940 | 0.079033 | 0.035036 | 0.168340 | 0.143826 | 0.065840 | 0.035692 |
Eisosomes | 0.000025 | 0.000010 | 0.000007 | 0.000005 | 0.000010 | 0.000011 | 0.000011 | 0.000005 | 0.000015 | 0.000011 | 0.000009 | 0.000005 |
Endoplasmic Reticulum | 0.016960 | 0.003518 | 0.001644 | 0.000375 | 0.006284 | 0.002779 | 0.000984 | 0.001288 | 0.009768 | 0.003063 | 0.001311 | 0.000936 |
Endosome | 0.020181 | 0.008105 | 0.011837 | 0.006239 | 0.006331 | 0.007289 | 0.018781 | 0.004938 | 0.010851 | 0.007602 | 0.015337 | 0.005439 |
Golgi | 0.023390 | 0.018809 | 0.018813 | 0.009871 | 0.016771 | 0.016809 | 0.004286 | 0.008984 | 0.018931 | 0.017576 | 0.011490 | 0.009326 |
Lipid Particles | 0.002243 | 0.000825 | 0.009163 | 0.009641 | 0.001043 | 0.000697 | 0.004423 | 0.005764 | 0.001435 | 0.000746 | 0.006774 | 0.007259 |
Mitochondria | 0.002757 | 0.002352 | 0.000081 | 0.000572 | 0.000843 | 0.001949 | 0.000370 | 0.000855 | 0.001468 | 0.002104 | 0.000226 | 0.000746 |
Mitotic Spindle | 0.001860 | 0.001038 | 0.017603 | 0.003023 | 0.000517 | 0.001158 | 0.007386 | 0.001795 | 0.000955 | 0.001112 | 0.012453 | 0.002269 |
None | 0.001769 | 0.000215 | 0.000592 | 0.000191 | 0.000407 | 0.000252 | 0.000213 | 0.000173 | 0.000852 | 0.000238 | 0.000401 | 0.000180 |
Nuclear Periphery | 0.000080 | 0.000184 | 0.000282 | 0.000033 | 0.000059 | 0.000021 | 0.000220 | 0.000025 | 0.000066 | 0.000083 | 0.000251 | 0.000028 |
Nuclear Periphery Foci | 0.001660 | 0.000872 | 0.011134 | 0.002125 | 0.000513 | 0.000231 | 0.004700 | 0.001404 | 0.000887 | 0.000477 | 0.007891 | 0.001682 |
Nucleolus | 0.000184 | 0.000356 | 0.000067 | 0.000107 | 0.000044 | 0.000045 | 0.000199 | 0.000080 | 0.000090 | 0.000165 | 0.000133 | 0.000090 |
Nucleus | 0.005591 | 0.002881 | 0.026169 | 0.003752 | 0.002498 | 0.001962 | 0.012418 | 0.002829 | 0.003508 | 0.002315 | 0.019237 | 0.003185 |
Peroxisomes | 0.001040 | 0.000554 | 0.000245 | 0.001202 | 0.000365 | 0.000495 | 0.000877 | 0.000557 | 0.000585 | 0.000518 | 0.000564 | 0.000805 |
Vacuole | 0.044461 | 0.019617 | 0.063661 | 0.065582 | 0.017411 | 0.021387 | 0.295041* | 0.061237 | 0.026240 | 0.020708 | 0.180295 | 0.062912 |
Vacuole Periphery | 0.038496 | 0.015285 | 0.006006 | 0.004353 | 0.013267 | 0.014796 | 0.004737 | 0.006177 | 0.021502 | 0.014984 | 0.005366 | 0.005473 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.01 | -2.29 | -0.38 | -1.21 | 1.73 | 1.37 | -0.79 | -1.81 | -3.51 | -0.30 | 1.30 | -2.96 | -1.39 | -2.47 | 1.79 |
Bud Neck | 7.74 | -7.76 | -3.61 | -6.88 | 5.72 | -1.77 | -5.44 | -8.21 | -6.59 | 4.16 | 3.93 | -9.69 | -7.35 | -9.16 | 7.39 |
Bud Site | 11.45 | 9.56 | 11.10 | 1.02 | -0.20 | -0.83 | 3.54 | -2.45 | -1.96 | -5.19 | 8.16 | 7.33 | 6.46 | -0.74 | -2.22 |
Cell Periphery | 5.37 | 1.15 | 0.81 | -3.04 | -0.19 | 2.42 | 3.21 | -6.00 | -6.20 | -6.41 | 5.21 | -0.12 | -3.42 | -6.31 | -2.85 |
Cytoplasm | -13.75 | -7.59 | -17.95 | -6.63 | -5.66 | -1.47 | 11.24 | -8.04 | -7.20 | -14.75 | -8.92 | 5.12 | -16.50 | -9.75 | -14.47 |
Cytoplasmic Foci | 4.85 | 17.55 | 20.34 | 21.06 | 2.94 | 2.95 | 11.06 | 23.28 | 23.45 | 5.73 | 4.94 | 20.05 | 30.55 | 31.45 | 6.41 |
Eisosomes | 2.83 | 3.05 | 3.80 | 3.73 | 2.91 | -0.18 | -0.61 | 5.00 | 6.04 | 4.47 | 2.44 | 2.41 | 5.47 | 7.01 | 5.45 |
Endoplasmic Reticulum | 4.92 | 5.73 | 6.20 | 5.72 | 5.74 | 3.90 | 7.01 | 5.21 | 2.05 | -0.65 | 6.20 | 8.33 | 8.19 | 4.26 | 0.81 |
Endosome | 8.20 | 4.39 | 8.52 | 1.72 | 2.87 | -1.46 | -5.57 | 1.89 | 2.96 | 6.18 | 4.87 | -2.18 | 7.23 | 3.36 | 6.59 |
Golgi | 2.89 | 2.74 | 8.13 | 6.29 | 2.27 | -0.04 | 10.65 | 6.79 | 6.86 | -3.94 | 1.59 | 6.34 | 10.23 | 9.28 | 0.19 |
Lipid Particles | 4.67 | -7.16 | -6.44 | -7.78 | 0.12 | 3.03 | -5.56 | -9.14 | -9.75 | -1.61 | 5.50 | -9.23 | -10.63 | -11.95 | -0.12 |
Mitochondria | 0.35 | 3.19 | 2.42 | 1.95 | -1.02 | -3.55 | 2.46 | 0.06 | 1.67 | -0.84 | -1.36 | 4.25 | 1.53 | 2.54 | -1.21 |
Mitotic Spindle | 1.32 | -5.46 | -1.28 | -2.34 | 4.92 | -1.52 | -3.82 | -2.52 | -1.12 | 3.14 | -0.48 | -6.77 | -2.79 | -2.37 | 5.90 |
None | 1.20 | 0.82 | 1.22 | 0.88 | 4.85 | 2.19 | 1.77 | 3.09 | 1.76 | 1.45 | 1.44 | 0.86 | 1.57 | 1.96 | 4.83 |
Nuclear Periphery | -0.83 | -5.67 | 1.39 | 1.18 | 6.74 | 0.91 | -5.01 | 0.73 | -1.11 | 7.37 | -0.32 | -7.27 | 1.11 | 1.07 | 9.77 |
Nuclear Periphery Foci | 1.26 | -8.92 | -1.37 | -3.26 | 8.85 | 2.63 | -6.35 | -7.06 | -10.48 | 5.08 | 1.77 | -11.31 | -4.67 | -7.00 | 10.10 |
Nucleolus | -0.68 | 1.62 | 1.21 | 0.96 | -1.40 | -0.21 | -6.16 | -5.01 | -4.86 | 4.45 | -0.76 | -2.27 | -0.21 | 0.73 | 3.31 |
Nucleus | 1.21 | -5.36 | 0.72 | -1.39 | 5.81 | 2.82 | -5.53 | -2.87 | -6.02 | 5.26 | 1.58 | -7.18 | -0.07 | -3.56 | 7.28 |
Peroxisomes | 5.10 | 8.00 | -0.59 | -2.28 | -3.26 | -3.57 | -3.22 | -4.02 | -1.54 | 1.35 | 1.68 | 1.01 | -2.00 | -2.63 | -2.38 |
Vacuole | 6.60 | -4.28 | -4.66 | -9.83 | 1.24 | -2.47 | -16.25 | -11.67 | -10.80 | 13.47 | 3.33 | -15.13 | -12.21 | -14.59 | 11.27 |
Vacuole Periphery | 7.24 | 10.92 | 11.59 | 7.46 | 0.64 | -0.92 | 7.31 | 5.04 | 6.65 | -2.83 | 4.21 | 12.30 | 11.68 | 9.72 | -1.50 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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