Standard name
Human Ortholog
Description Mitochondrial intermembrane space protein of unknown function; imported via the MIA import machinery; contains an unusual twin cysteine motif (CX13C CX14C)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.94 0.99 0.99 0.97 0.94 0.9 – 0.79 0.81 0.69 0.78 0.99 0.98 0.99 0.97 0.93 0.97 0.82 0.82 0.86 0.85 0.76 0.68
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0.07 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0 0.09 0 – 0.29 0.23 0.41 0.3 0 0 0 0 0 0.05 0.06 0.05 0.08 0 0.08 0.1
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 2 2 2 1 6 9
Bud 0 1 0 0 0 3 4 – 6 5 6 14 0 0 0 0 0 0 1 1 0 1 8 12
Bud Neck 0 0 2 3 9 5 14 – 13 12 3 8 0 0 0 1 0 0 2 2 0 0 2 4
Bud Site 0 0 0 0 0 1 0 – 0 0 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 3 6 3 1 1 – 4 1 2 2 1 2 1 5 2 2 1 1 1 0 1 1
Cytoplasm 174 126 248 263 388 295 509 – 341 348 241 372 244 446 457 235 140 113 158 116 217 157 245 229
Endoplasmic Reticulum 1 3 0 0 0 1 0 – 0 0 0 0 0 3 1 11 11 10 3 0 1 5 2 3
Endosome 5 0 0 0 2 0 5 – 1 1 1 0 0 4 1 2 3 1 5 3 1 0 3 9
Golgi 0 1 0 0 1 0 0 – 0 0 0 0 0 1 1 3 3 2 0 1 0 1 3 1
Mitochondria 2 7 0 5 3 27 25 – 125 98 141 142 2 0 0 4 0 6 11 6 19 7 25 34
Nucleus 0 0 0 1 2 3 9 – 9 0 4 2 0 1 1 2 2 1 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 1 0 0 0 0 1 0 1
SpindlePole 0 0 1 1 3 1 25 – 11 12 8 20 0 1 0 0 1 0 0 2 2 0 9 14
Vac/Vac Membrane 4 1 0 0 5 0 14 – 3 2 2 2 2 1 1 3 6 1 3 2 0 0 5 7
Unique Cell Count 185 134 250 267 401 313 566 429 429 347 475 246 455 461 243 150 117 194 141 253 186 325 340
Labelled Cell Count 186 139 254 279 416 337 606 513 481 410 563 249 460 463 266 170 136 194 141 253 186 325 340


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 4.3 5.3 4.9 5.3 3.6 4.3 – 3.5 3.7 3.5 3.6 5.1 5.2 5.2 5.4 6.2 5.7 4.6 5.0 5.1
Std Deviation (1e-4) 0.5 0.8 1.0 1.4 1.5 1.0 1.6 – 1.3 1.0 1.0 1.0 1.1 1.4 1.6 1.2 2.0 1.8 1.5 2.3 2.3
Intensity Change (Log2) – – – -0.12 -0.01 -0.57 -0.29 – -0.58 -0.51 -0.6 -0.56 -0.05 -0.03 -0.03 0.04 0.23 0.1 -0.19 -0.08 -0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1203 0.0594 -0.1537 -0.3397 -0.1042 0.2589 0.5191 0.9937 0.76 0.4671 1.0725 0.8534 2.3894 3.5982 2.6197 2.7222 3.3714 3.0425
Actin 0.0501 0.0015 0.0301 0.0083 0.029 0.0156 0.0403 0.0021 0.0192 0.0077 0.0209 0.01 0.0313 0.0002 0.0016 0.0205 0.0844 0.0011
Bud 0.0007 0.0032 0.0059 0.0016 0.0011 0.0368 0.0033 0.0024 0.0031 0.0009 0.0008 0.0031 0.0004 0 0.0014 0.0002 0.0004 0.0017
Bud Neck 0.0059 0.0001 0.0023 0.0004 0.0004 0.0017 0.0016 0.0011 0.0013 0.0005 0.0009 0.0021 0.0006 0 0.0004 0.0001 0.0009 0.0004
Bud Periphery 0.0007 0.0012 0.0017 0.0017 0.0016 0.0213 0.005 0.0018 0.0059 0.0013 0.0008 0.0048 0.0006 0 0.001 0.0004 0.0007 0.0071
Bud Site 0.0107 0.0029 0.0064 0.0367 0.0004 0.003 0.005 0.0061 0.0094 0.0013 0.0013 0.001 0.001 0.0001 0.0002 0.0001 0.003 0.0001
Cell Periphery 0.0002 0.0002 0.0004 0.0004 0.0001 0.0006 0.0002 0.0002 0.0004 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001
Cytoplasm 0.1932 0.3115 0.2978 0.4022 0.2923 0.5128 0.3031 0.4585 0.4242 0.4719 0.4237 0.5382 0.2861 0.4052 0.4421 0.4088 0.4094 0.5127
Cytoplasmic Foci 0.04 0.0094 0.0098 0.0058 0.0783 0.0124 0.0354 0.0177 0.0125 0.0283 0.0277 0.0278 0.0502 0.002 0.0045 0.0217 0.0051 0.0085
Eisosomes 0.0004 0.0001 0.0002 0.0001 0.0002 0.0001 0.0006 0.0001 0.0003 0.0001 0.0002 0.0001 0.0002 0.0001 0.0003 0.0001 0.0007 0
Endoplasmic Reticulum 0.0018 0.0021 0.0028 0.002 0.0011 0.0034 0.0047 0.002 0.004 0.0016 0.0073 0.0024 0.0017 0.0022 0.0037 0.0033 0.0015 0.0023
Endosome 0.0149 0.001 0.0186 0.0021 0.0019 0.0187 0.0213 0.0042 0.0117 0.0205 0.0326 0.007 0.015 0.0004 0.0052 0.013 0.002 0.0022
Golgi 0.0145 0.0002 0.0112 0.001 0.0078 0.0021 0.0061 0.0009 0.0047 0.0038 0.005 0.0021 0.0053 0.0001 0.0004 0.0089 0.0038 0.0002
Lipid Particles 0.0116 0.0006 0.0075 0.0014 0.0191 0.0018 0.0124 0.0022 0.0039 0.0056 0.0047 0.0055 0.0104 0.0001 0.0002 0.0155 0.0057 0.0002
Mitochondria 0.0042 0.0018 0.0054 0.0004 0.0036 0.0061 0.0063 0.0009 0.011 0.006 0.004 0.0083 0.0047 0.0001 0.0006 0.0021 0.0008 0.0008
None 0.6185 0.6602 0.584 0.5315 0.5181 0.3336 0.519 0.4837 0.4647 0.4016 0.4122 0.3622 0.5501 0.5881 0.5237 0.4944 0.4623 0.4566
Nuclear Periphery 0.0098 0.0007 0.0049 0.0007 0.0006 0.0098 0.0063 0.0012 0.0046 0.0013 0.0165 0.0038 0.0051 0.0004 0.006 0.002 0.0005 0.0016
Nucleolus 0.0005 0.0003 0.0007 0.0002 0.0061 0.0011 0.0005 0.0015 0.0007 0.0003 0.0015 0.0006 0.0004 0 0.0009 0.0001 0.0001 0.0002
Nucleus 0.0041 0.001 0.0024 0.001 0.0011 0.0033 0.0045 0.0055 0.0041 0.0029 0.0098 0.0045 0.0038 0.0005 0.0028 0.0007 0.0005 0.0023
Peroxisomes 0.0104 0.0003 0.0028 0.0006 0.0337 0.0036 0.0138 0.0036 0.0047 0.0385 0.0018 0.0057 0.0262 0 0.0001 0.0058 0.0167 0.0003
Punctate Nuclear 0.0061 0.0009 0.0021 0.001 0.0021 0.0095 0.0078 0.003 0.0025 0.0035 0.0233 0.0088 0.0055 0.0002 0.0036 0.0015 0.0008 0.0004
Vacuole 0.001 0.0007 0.0021 0.0008 0.0006 0.0017 0.002 0.0013 0.0053 0.0018 0.0032 0.001 0.0007 0.0003 0.0007 0.0003 0.0003 0.0007
Vacuole Periphery 0.0006 0.0001 0.0009 0.0001 0.0008 0.001 0.0009 0.0002 0.0017 0.0005 0.0017 0.0006 0.0005 0 0.0005 0.0004 0.0001 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.6036 20.0279 14.8616 29.9406 31.4686 24.2577 23.6253 34.1689 29.4292 26.8001
Translational Efficiency 1.2564 1.127 1.6635 0.9311 0.6764 1.8246 1.372 0.8321 1.3865 1.1326

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1586 1074 1600 89 1958 2197 2259 1473 3544 3271 3859 1562

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 687.69 724.42 799.88 877.38 681.91 774.30 848.69 893.58 684.50 757.92 828.45 892.66
Standard Deviation 101.79 103.20 102.96 95.71 78.06 93.04 96.19 128.09 89.51 99.30 101.93 126.52
Intensity Change Log 2 — 0.075068 0.218025 0.351443 — 0.183311 0.315656 0.390016 — 0.129976 0.267461 0.370777

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000169 0.000312 0.000210 0.000692 0.000105 0.000329 0.000064 0.002396 0.000134 0.000323 0.000124 0.002299
Bud Neck 0.020056 0.024919 0.004301 0.008206 0.009877 0.033008 0.005406 0.014898 0.014432 0.030352 0.004948 0.014517
Bud Site 0.004037 0.013145 0.005893 0.024814 0.003374 0.018414 0.003760 0.033085 0.003670 0.016684 0.004645 0.032613
Cell Periphery 0.000125 0.000131 0.000108 0.000343 0.000109 0.000170 0.000054 0.000153 0.000116 0.000157 0.000077 0.000164
Cytoplasm 0.447655* 0.460823* 0.388130* 0.424431* 0.539380* 0.502354* 0.626939* 0.532959* 0.498331* 0.488718* 0.527925* 0.526776*
Cytoplasmic Foci 0.236686* 0.236493* 0.006736 0.056335 0.188640* 0.188812* 0.009677 0.023351 0.210141* 0.204468* 0.008458 0.025231
Eisosomes 0.000202 0.000102 0.000022 0.000104 0.000129 0.000133 0.000014 0.000037 0.000162 0.000123 0.000017 0.000041
Endoplasmic Reticulum 0.001639 0.001726 0.002927 0.006066 0.000533 0.000619 0.000930 0.002546 0.001028 0.000982 0.001758 0.002747
Endosome 0.009554 0.020250 0.001179 0.010089 0.006253 0.012607 0.000921 0.008466 0.007730 0.015117 0.001028 0.008558
Golgi 0.003153 0.002758 0.000063 0.000208 0.001436 0.003837 0.000039 0.008021 0.002204 0.003483 0.000049 0.007576
Lipid Particles 0.010278 0.006670 0.000730 0.003924 0.005408 0.005023 0.000388 0.001216 0.007587 0.005564 0.000530 0.001371
Mitochondria 0.006924 0.006817 0.000538 0.000236 0.002086 0.006792 0.000432 0.005863 0.004251 0.006800 0.000476 0.005543
Mitotic Spindle 0.001158 0.002291 0.003890 0.011835 0.001578 0.007868 0.002978 0.050809 0.001390 0.006037 0.003356 0.048588
None 0.003102 0.003768 0.005661 0.001637 0.003667 0.004028 0.003853 0.007772 0.003414 0.003943 0.004603 0.007423
Nuclear Periphery 0.000597 0.000521 0.001520 0.001055 0.000355 0.000283 0.001217 0.001680 0.000463 0.000361 0.001343 0.001645
Nuclear Periphery Foci 0.000330 0.000883 0.000890 0.004710 0.000345 0.000251 0.000334 0.001674 0.000338 0.000458 0.000564 0.001847
Nucleolus 0.001140 0.002356 0.000343 0.000365 0.000880 0.001585 0.000174 0.000584 0.000996 0.001838 0.000244 0.000571
Nucleus 0.199172* 0.128397 0.549430* 0.215826* 0.195513* 0.145584* 0.308046* 0.204180* 0.197151* 0.139941* 0.408128* 0.204844*
Peroxisomes 0.006660 0.008778 0.000140 0.001204 0.003018 0.010155 0.000133 0.004448 0.004648 0.009703 0.000136 0.004264
Vacuole 0.046254 0.075925 0.027178 0.227536* 0.036855 0.056823 0.034585 0.091610 0.041061 0.063095 0.031514 0.099355
Vacuole Periphery 0.001111 0.002936 0.000115 0.000385 0.000459 0.001325 0.000054 0.004248 0.000750 0.001854 0.000079 0.004028

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.79 -2.55 -5.38 -3.90 -4.63 -6.42 1.30 -2.43 -1.83 -2.49 -6.92 -1.29 -2.45 -1.90 -2.39
Bud Neck -1.83 10.58 5.74 6.09 -1.23 -12.61 4.24 -0.14 11.57 -3.50 -10.46 10.76 3.71 12.18 -4.10
Bud Site -7.10 -1.58 -2.78 -0.58 -2.23 -10.16 0.22 -7.70 0.36 -7.53 -12.09 -1.05 -7.95 -0.40 -7.20
Cell Periphery -0.78 1.24 -2.14 -2.02 -2.33 -4.31 11.08 -0.21 3.97 -8.26 -4.14 8.10 -0.25 3.40 -5.31
Cytoplasm -0.94 10.02 5.61 5.85 2.15 4.63 -2.24 14.81 10.52 16.85 1.69 4.11 12.22 10.51 8.91
Cytoplasmic Foci -0.06 41.52 21.31 19.90 -5.29 0.01 40.64 37.24 37.15 -6.62 1.10 57.67 51.02 47.31 -9.26
Eisosomes 7.94 14.87 4.39 -1.55 -7.37 -0.40 17.99 12.15 14.04 -17.02 5.25 22.21 16.20 15.33 -16.40
Endoplasmic Reticulum -0.18 -4.28 -4.17 -4.35 -2.39 -0.87 -9.52 -15.72 -13.76 -10.37 0.14 -5.74 -8.70 -12.06 -5.35
Endosome -6.23 11.26 0.68 4.71 -3.30 -6.88 11.22 3.51 8.88 -5.93 -8.63 15.71 5.63 12.14 -6.66
Golgi 1.04 10.35 9.78 8.85 -4.87 -4.81 6.99 -1.00 1.64 -2.71 -3.48 12.39 0.04 1.55 -2.71
Lipid Particles 4.32 14.54 7.43 3.88 -5.14 0.66 11.00 8.23 7.93 -14.81 4.00 18.14 14.80 11.44 -13.60
Mitochondria 0.17 6.15 6.44 6.71 4.33 -6.02 3.53 -0.10 5.07 -2.57 -3.36 7.08 2.70 5.77 -2.48
Mitotic Spindle -0.69 -2.09 -1.75 -1.70 -1.55 -4.41 -1.87 -9.81 -7.61 -9.41 -4.38 -2.78 -10.02 -8.47 -9.44
None -0.82 -3.41 -0.43 -0.23 0.25 -0.52 -0.35 -5.10 -4.81 -6.95 -0.99 -2.50 -5.96 -5.25 -5.35
Nuclear Periphery 0.59 -12.32 -6.27 -6.83 0.30 1.64 -8.20 -14.84 -15.38 -5.79 1.92 -12.26 -14.51 -15.31 -6.47
Nuclear Periphery Foci -4.80 -7.12 -4.14 -3.66 -3.52 2.30 -1.88 -7.70 -8.01 -7.39 -2.61 -6.90 -8.45 -8.00 -7.21
Nucleolus -3.43 4.45 1.85 4.74 -1.65 -2.87 5.16 -1.12 2.15 -6.55 -4.06 6.74 -0.17 3.86 -6.45
Nucleus 10.23 -42.71 -1.23 -3.80 12.21 9.32 -24.35 -10.49 -17.94 10.09 13.48 -44.66 -10.83 -19.64 23.13
Peroxisomes -2.51 10.79 8.38 10.86 -3.45 -9.30 10.41 2.36 9.58 -2.74 -8.33 14.46 5.19 11.20 -2.84
Vacuole -8.15 2.13 -10.56 -9.42 -10.77 -8.06 -8.52 -26.37 -22.61 -21.92 -10.70 -5.36 -27.55 -23.44 -25.44
Vacuole Periphery -3.91 8.76 7.00 5.99 -2.55 -7.21 11.61 -2.28 0.15 -3.44 -6.30 12.32 -1.36 1.67 -3.39
Standard name
Human Ortholog
Description Mitochondrial intermembrane space protein of unknown function; imported via the MIA import machinery; contains an unusual twin cysteine motif (CX13C CX14C)
Localization
Cell Percentages cytoplasm (32%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Mix23

Mix23


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mix23-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available