Standard name | |
---|---|
Human Ortholog | |
Description | Protein serine/threonine kinase; essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 128 | 2 | 32 | 56 | 59 | 51 | 47 | 40 | 16 | 22 | 19 | 8 | 9 | 36 | 35 | 25 | 27 | 19 | 85 | 4 | 14 | 10 | 32 | 43 |
Bud | 86 | 0 | 27 | 21 | 70 | 56 | 64 | 75 | 34 | 43 | 27 | 35 | 9 | 25 | 33 | 13 | 12 | 8 | 160 | 16 | 39 | 71 | 121 | 133 |
Bud Neck | 5 | 0 | 0 | 3 | 7 | 6 | 13 | 15 | 1 | 6 | 1 | 2 | 0 | 0 | 0 | 1 | 2 | 1 | 91 | 12 | 22 | 55 | 72 | 57 |
Bud Site | 57 | 3 | 12 | 33 | 60 | 51 | 38 | 23 | 16 | 22 | 13 | 11 | 2 | 2 | 2 | 3 | 3 | 4 | – | – | – | – | – | – |
Cell Periphery | 53 | 3 | 1 | 26 | 15 | 25 | 36 | 41 | 32 | 32 | 21 | 37 | 31 | 84 | 96 | 15 | 20 | 12 | 23 | 1 | 2 | 1 | 1 | 8 |
Cytoplasm | 213 | 16 | 50 | 41 | 77 | 74 | 108 | 150 | 85 | 110 | 56 | 97 | 163 | 213 | 242 | 80 | 96 | 71 | 124 | 24 | 29 | 12 | 55 | 55 |
Endoplasmic Reticulum | 8 | 1 | 1 | 1 | 1 | 3 | 4 | 10 | 5 | 4 | 4 | 2 | 3 | 7 | 2 | 2 | 10 | 3 | 5 | 0 | 0 | 0 | 1 | 3 |
Endosome | 5 | 0 | 0 | 1 | 8 | 3 | 1 | 10 | 1 | 1 | 0 | 1 | 2 | 0 | 2 | 1 | 6 | 4 | 2 | 1 | 3 | 1 | 4 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 0 | 1 | 0 | 0 | 0 |
Mitochondria | 3 | 3 | 30 | 57 | 38 | 71 | 122 | 200 | 120 | 149 | 86 | 140 | 1 | 1 | 21 | 9 | 0 | 6 | 4 | 0 | 4 | 2 | 1 | 4 |
Nucleus | 6 | 0 | 0 | 2 | 17 | 17 | 11 | 15 | 4 | 9 | 9 | 9 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 2 | 3 | 5 | 3 | 2 | 1 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 4 | 0 | 1 | 0 | 6 | 9 | 13 | 44 | 5 | 12 | 2 | 14 | 1 | 0 | 0 | 2 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 14 | 1 | 3 | 4 | 11 | 9 | 7 | 9 | 4 | 6 | 0 | 1 | 1 | 0 | 2 | 1 | 3 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 25 | 0 | 0 | 3 | 28 | 20 | 19 | 27 | 3 | 5 | 3 | 5 | 0 | 0 | 0 | 5 | 9 | 14 | 4 | 0 | 2 | 3 | 4 | 2 |
Vac/Vac Membrane | 7 | 1 | 3 | 1 | 12 | 9 | 13 | 41 | 8 | 11 | 9 | 19 | 1 | 0 | 2 | 7 | 8 | 10 | 0 | 0 | 3 | 1 | 1 | 4 |
Unique Cell Count | 441 | 25 | 110 | 154 | 272 | 254 | 332 | 501 | 233 | 304 | 178 | 257 | 185 | 269 | 318 | 123 | 152 | 125 | 515 | 64 | 128 | 162 | 303 | 322 |
Labelled Cell Count | 615 | 30 | 160 | 249 | 409 | 406 | 499 | 705 | 337 | 434 | 251 | 391 | 223 | 368 | 437 | 165 | 200 | 160 | 515 | 64 | 128 | 162 | 303 | 322 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.6 | 7.2 | 5.0 | 5.0 | 5.5 | 5.1 | 4.8 | 4.9 | 4.4 | 4.4 | 4.6 | 4.2 | 8.1 | 7.9 | 6.7 | 9.8 | 10.3 | 11.2 | 7.8 | 7.4 | 7.7 |
Std Deviation (1e-4) | 1.7 | 1.3 | 1.0 | 1.0 | 1.2 | 1.7 | 1.4 | 1.2 | 1.5 | 1.2 | 1.3 | 1.1 | 1.6 | 1.5 | 1.2 | 1.9 | 2.0 | 2.2 | 1.6 | 1.7 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.14 | 0.04 | -0.06 | -0.02 | -0.17 | -0.17 | -0.13 | -0.24 | 0.7 | 0.66 | 0.42 | 0.98 | 1.05 | 1.17 | 0.64 | 0.57 | 0.63 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.2 | -1.5 | -1.9 | 0 | -6.2 | 0 | 0 | 0 | 0 | -5.8 | -3.6 | -4.5 | -1.6 | -2.2 | -2.6 |
Bud | -2.3 | 0.2 | -0.5 | 0 | -2.4 | 0 | 0 | 0 | 0 | -5.0 | -3.9 | -3.7 | -2.8 | -3.7 | -3.9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 2.2 | 2.5 | 2.1 | 0 | -2.6 | 0 | 0 | 0 | 0 | -3.8 | 0 | 0 | -2.6 | -3.1 | -2.3 |
Cell Periphery | 4.2 | 0 | 3.0 | 0 | 2.7 | 0 | 0 | 0 | 0 | 4.2 | 6.4 | 6.3 | 3.4 | 3.6 | 2.9 |
Cytoplasm | -3.2 | -3.2 | -3.0 | -2.5 | -3.1 | -1.6 | -1.7 | -2.4 | -1.4 | 7.9 | 6.5 | 6.0 | 3.0 | 2.8 | 1.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.7 | -3.1 | 0.1 | 0 | 2.5 | 0 | 0 | 0 | 0 | -7.2 | -8.7 | -5.8 | -4.1 | -6.8 | -4.8 |
Nucleus | 0 | 0 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 3.5 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.6 |
Vacuole | 0 | 0 | 0 | 0 | 2.0 | 0 | 0 | 0 | 1.7 | 0 | 0 | 0 | 0 | 0 | 1.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.2723 | 4.5553 | 4.2313 | 4.0979 | 3.9769 | 4.5626 | 6.0746 | 6.5938 | 6.2101 | 5.5624 | 5.71 | 6.566 | 3.2783 | 3.4973 | 3.2403 | 4.0345 | 3.2764 | 3.5323 |
Actin | 0.03 | 0.0163 | 0.035 | 0.0563 | 0.03 | 0.0155 | 0.0789 | 0.0076 | 0.0256 | 0.0434 | 0.0453 | 0.0138 | 0.0386 | 0.0104 | 0.0321 | 0.0229 | 0.0288 | 0.0165 |
Bud | 0.0109 | 0.0196 | 0.2207 | 0.1064 | 0.0816 | 0.0102 | 0.0431 | 0.0319 | 0.2133 | 0.1116 | 0.0432 | 0.0015 | 0.0331 | 0.0463 | 0.1817 | 0.0863 | 0.05 | 0.0068 |
Bud Neck | 0.105 | 0.0125 | 0.009 | 0.0011 | 0.0018 | 0.6872 | 0.0297 | 0.0059 | 0.0042 | 0.0049 | 0.1002 | 0.6738 | 0.1146 | 0.0095 | 0.0028 | 0.004 | 0.0386 | 0.5453 |
Bud Periphery | 0.0209 | 0.0162 | 0.2629 | 0.5163 | 0.3379 | 0.0176 | 0.057 | 0.02 | 0.2338 | 0.2337 | 0.081 | 0.0031 | 0.0715 | 0.0516 | 0.2607 | 0.4044 | 0.1265 | 0.0147 |
Bud Site | 0.1671 | 0.4153 | 0.126 | 0.0036 | 0.0039 | 0.0086 | 0.157 | 0.3521 | 0.0431 | 0.0111 | 0.0162 | 0.0066 | 0.1854 | 0.3746 | 0.0613 | 0.052 | 0.0093 | 0.0064 |
Cell Periphery | 0.0113 | 0.0101 | 0.0075 | 0.0098 | 0.0124 | 0.0019 | 0.0159 | 0.0034 | 0.0023 | 0.0034 | 0.0107 | 0.0022 | 0.0211 | 0.002 | 0.004 | 0.0071 | 0.0125 | 0.0017 |
Cytoplasm | 0.5739 | 0.4759 | 0.3083 | 0.2898 | 0.5125 | 0.2385 | 0.4888 | 0.5641 | 0.3972 | 0.308 | 0.5748 | 0.2527 | 0.4349 | 0.4798 | 0.3726 | 0.4069 | 0.5842 | 0.3771 |
Cytoplasmic Foci | 0.0106 | 0.0092 | 0.0061 | 0.0021 | 0.0013 | 0.0058 | 0.0231 | 0.0042 | 0.0115 | 0.0199 | 0.042 | 0.0109 | 0.0147 | 0.0032 | 0.0082 | 0.0013 | 0.0109 | 0.0183 |
Eisosomes | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0005 | 0 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0003 | 0.0001 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0067 | 0.003 | 0.0043 | 0.0035 | 0.0057 | 0.0013 | 0.0064 | 0.0014 | 0.0026 | 0.0058 | 0.003 | 0.0012 | 0.0078 | 0.0014 | 0.0019 | 0.0047 | 0.0035 | 0.0011 |
Endosome | 0.0038 | 0.0016 | 0.0008 | 0.0024 | 0.0013 | 0.001 | 0.0307 | 0.0014 | 0.0179 | 0.0875 | 0.0461 | 0.0043 | 0.012 | 0.0026 | 0.0037 | 0.0013 | 0.0187 | 0.0016 |
Golgi | 0.0019 | 0.0008 | 0.0007 | 0.0029 | 0.0008 | 0.0009 | 0.0129 | 0.0009 | 0.0181 | 0.0294 | 0.0055 | 0.006 | 0.0092 | 0.0006 | 0.0044 | 0.0003 | 0.0136 | 0.0016 |
Lipid Particles | 0.0012 | 0.0005 | 0.0003 | 0.0001 | 0.0002 | 0.0004 | 0.0081 | 0.0001 | 0.0036 | 0.0184 | 0.0036 | 0.0024 | 0.011 | 0.0001 | 0.0014 | 0.0003 | 0.0176 | 0.0005 |
Mitochondria | 0.0003 | 0.0002 | 0.0005 | 0.0012 | 0.0008 | 0.0002 | 0.0141 | 0.0008 | 0.0121 | 0.0928 | 0.001 | 0.0068 | 0.0152 | 0.0003 | 0.0236 | 0.0004 | 0.0082 | 0.0003 |
None | 0.0316 | 0.0124 | 0.0108 | 0.0007 | 0.001 | 0.0055 | 0.0048 | 0.0029 | 0.0045 | 0.0034 | 0.0032 | 0.0025 | 0.0067 | 0.0024 | 0.0329 | 0.0028 | 0.0659 | 0.0008 |
Nuclear Periphery | 0.0009 | 0.0004 | 0.0007 | 0.0007 | 0.0014 | 0.0003 | 0.0048 | 0.0004 | 0.0006 | 0.0037 | 0.0058 | 0.0002 | 0.0019 | 0.0006 | 0.0006 | 0.0006 | 0.0008 | 0.0001 |
Nucleolus | 0.001 | 0.0003 | 0.0004 | 0 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0006 | 0.0003 | 0.0001 | 0.0004 | 0.0003 | 0.0002 | 0.0005 | 0.0002 | 0.0002 |
Nucleus | 0.0015 | 0.0017 | 0.0013 | 0.0008 | 0.0014 | 0.001 | 0.0031 | 0.0012 | 0.0011 | 0.0024 | 0.003 | 0.0004 | 0.0012 | 0.0032 | 0.0014 | 0.001 | 0.0009 | 0.0005 |
Peroxisomes | 0.0013 | 0.0004 | 0.0005 | 0.0001 | 0.0001 | 0.0004 | 0.0103 | 0.0006 | 0.0051 | 0.009 | 0.0028 | 0.0102 | 0.0156 | 0.0001 | 0.0041 | 0.0001 | 0.0054 | 0.0056 |
Punctate Nuclear | 0.0015 | 0.0007 | 0.0009 | 0.0001 | 0.0001 | 0.0003 | 0.0033 | 0.0005 | 0.0003 | 0.0011 | 0.0088 | 0.0004 | 0.0019 | 0.0002 | 0.0004 | 0.0005 | 0.0009 | 0.0004 |
Vacuole | 0.017 | 0.0025 | 0.0027 | 0.0016 | 0.005 | 0.003 | 0.0057 | 0.0006 | 0.002 | 0.0068 | 0.0029 | 0.0004 | 0.0018 | 0.0102 | 0.0015 | 0.0023 | 0.0028 | 0.0007 |
Vacuole Periphery | 0.0012 | 0.0001 | 0.0002 | 0.0004 | 0.0008 | 0.0002 | 0.0012 | 0.0001 | 0.0008 | 0.0022 | 0.0005 | 0.0002 | 0.0009 | 0.0005 | 0.0003 | 0.0001 | 0.0004 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 21.8701 | 27.1139 | 27.5322 | 32.2406 | 23.3882 | 17.5502 | 26.6213 | 30.2868 | 34.4656 | 30.8759 |
Translational Efficiency | 0.5967 | 0.5221 | 0.6157 | 0.5552 | 0.4967 | 0.7618 | 0.5833 | 0.5547 | 0.4738 | 0.4797 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1635 | 108 | 186 | 1164 | 1487 | 1823 | 441 | 668 | 3122 | 1931 | 627 | 1832 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 755.90 | 856.22 | 1424.59 | 1096.36 | 798.98 | 918.14 | 1245.44 | 1143.01 | 776.42 | 914.68 | 1298.58 | 1113.37 |
Standard Deviation | 135.15 | 156.41 | 194.50 | 154.84 | 140.82 | 149.52 | 144.55 | 153.49 | 139.55 | 150.59 | 180.60 | 155.97 |
Intensity Change Log 2 | — | 0.179786 | 0.914279 | 0.536454 | — | 0.200555 | 0.640424 | 0.516607 | — | 0.190496 | 0.780053 | 0.526290 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.005894 | 0.009741 | 0.006500 | 0.012784 | 0.002319 | 0.003308 | 0.010816 | 0.011264 | 0.004191 | 0.003668 | 0.009536 | 0.012230 |
Bud Neck | 0.122323 | 0.077173 | 0.060117 | 0.044619 | 0.091231 | 0.115369 | 0.061932 | 0.046927 | 0.107514 | 0.113232 | 0.061394 | 0.045460 |
Bud Site | 0.380800* | 0.372269* | 0.510211* | 0.461502* | 0.285359* | 0.502751* | 0.569427* | 0.541168* | 0.335342* | 0.495453* | 0.551861* | 0.490551* |
Cell Periphery | 0.006301 | 0.000695 | 0.001196 | 0.001150 | 0.001284 | 0.002167 | 0.000877 | 0.001203 | 0.003911 | 0.002085 | 0.000972 | 0.001169 |
Cytoplasm | 0.331046* | 0.270626* | 0.192841* | 0.329389* | 0.441895* | 0.288256* | 0.218464* | 0.315042* | 0.383843* | 0.287270* | 0.210863* | 0.324158* |
Cytoplasmic Foci | 0.053836 | 0.109659 | 0.046136 | 0.021101 | 0.080033 | 0.033559 | 0.022471 | 0.015065 | 0.066313 | 0.037815 | 0.029492 | 0.018900 |
Eisosomes | 0.000923 | 0.000207 | 0.000158 | 0.000149 | 0.000278 | 0.000201 | 0.000126 | 0.000108 | 0.000616 | 0.000201 | 0.000135 | 0.000134 |
Endoplasmic Reticulum | 0.001780 | 0.001456 | 0.001407 | 0.000535 | 0.001484 | 0.000551 | 0.001000 | 0.000375 | 0.001639 | 0.000602 | 0.001121 | 0.000477 |
Endosome | 0.009977 | 0.042320 | 0.005742 | 0.007483 | 0.010874 | 0.006875 | 0.001451 | 0.001654 | 0.010404 | 0.008858 | 0.002724 | 0.005357 |
Golgi | 0.005079 | 0.004350 | 0.000435 | 0.005118 | 0.002997 | 0.004095 | 0.000186 | 0.001916 | 0.004087 | 0.004110 | 0.000260 | 0.003951 |
Lipid Particles | 0.002627 | 0.004162 | 0.003314 | 0.001375 | 0.001756 | 0.000690 | 0.000750 | 0.000451 | 0.002212 | 0.000884 | 0.001510 | 0.001038 |
Mitochondria | 0.011740 | 0.002133 | 0.000081 | 0.004576 | 0.001412 | 0.004030 | 0.000078 | 0.000312 | 0.006821 | 0.003924 | 0.000079 | 0.003022 |
Mitotic Spindle | 0.001268 | 0.001160 | 0.003520 | 0.010411 | 0.001655 | 0.002892 | 0.004051 | 0.008073 | 0.001452 | 0.002795 | 0.003894 | 0.009558 |
None | 0.005063 | 0.000086 | 0.000103 | 0.000186 | 0.000828 | 0.000349 | 0.000291 | 0.000080 | 0.003046 | 0.000334 | 0.000235 | 0.000148 |
Nuclear Periphery | 0.000036 | 0.000131 | 0.000175 | 0.000076 | 0.000061 | 0.000032 | 0.000079 | 0.000048 | 0.000048 | 0.000037 | 0.000107 | 0.000065 |
Nuclear Periphery Foci | 0.000384 | 0.000576 | 0.006779 | 0.000642 | 0.000489 | 0.000271 | 0.001663 | 0.000860 | 0.000434 | 0.000288 | 0.003181 | 0.000721 |
Nucleolus | 0.000485 | 0.000364 | 0.000050 | 0.000052 | 0.000285 | 0.000365 | 0.000059 | 0.000025 | 0.000389 | 0.000365 | 0.000056 | 0.000042 |
Nucleus | 0.013712 | 0.008287 | 0.010323 | 0.007962 | 0.015166 | 0.003821 | 0.012614 | 0.004185 | 0.014405 | 0.004071 | 0.011934 | 0.006585 |
Peroxisomes | 0.004498 | 0.015784 | 0.003265 | 0.004347 | 0.002615 | 0.002935 | 0.006201 | 0.004228 | 0.003601 | 0.003654 | 0.005330 | 0.004303 |
Vacuole | 0.040631 | 0.077091 | 0.147459 | 0.084676 | 0.056658 | 0.026050 | 0.087389 | 0.045581 | 0.048265 | 0.028905 | 0.105209 | 0.070421 |
Vacuole Periphery | 0.001598 | 0.001731 | 0.000189 | 0.001866 | 0.001323 | 0.001432 | 0.000074 | 0.001437 | 0.001467 | 0.001449 | 0.000108 | 0.001710 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.74 | -0.75 | -2.98 | -0.34 | -2.11 | -1.81 | -2.63 | -4.04 | -3.49 | -0.49 | 0.87 | -2.24 | -5.04 | -5.35 | -1.29 |
Bud Neck | 2.79 | 10.61 | 14.82 | 2.61 | 0.76 | -3.36 | 7.28 | 9.08 | 12.89 | 1.15 | -0.90 | 12.81 | 17.27 | 14.98 | 1.71 |
Bud Site | 0.61 | 2.23 | 2.53 | 0.35 | -1.02 | -18.77 | -5.34 | -8.44 | 5.08 | -1.47 | -16.09 | -2.03 | -3.84 | 10.46 | -0.42 |
Cell Periphery | 7.17 | 6.64 | 6.73 | -1.08 | 0.61 | -2.34 | 2.79 | 0.96 | 2.78 | -0.88 | 3.48 | 7.24 | 6.68 | 3.12 | -0.52 |
Cytoplasm | 2.01 | 4.05 | -4.10 | -3.69 | -6.25 | 13.88 | 10.56 | 5.47 | -5.15 | -4.75 | 10.61 | 9.41 | 1.14 | -8.36 | -7.98 |
Cytoplasmic Foci | -3.58 | 0.29 | 8.43 | 5.39 | 4.23 | 11.96 | 12.36 | 15.83 | 5.03 | 2.36 | 9.61 | 9.58 | 16.84 | 5.32 | 3.61 |
Eisosomes | 6.19 | 7.90 | 7.83 | 0.95 | 0.02 | 1.52 | 4.00 | 4.39 | 2.51 | 1.04 | 6.54 | 8.88 | 8.64 | 2.01 | 0.13 |
Endoplasmic Reticulum | 0.53 | -0.27 | 3.77 | 1.46 | 5.30 | 3.20 | -0.13 | 3.59 | 0.50 | 5.57 | 4.48 | 0.03 | 5.09 | 0.40 | 6.99 |
Endosome | -2.96 | 2.37 | 3.20 | 3.40 | 1.10 | 3.58 | 10.18 | 10.30 | 6.55 | 1.36 | 1.42 | 9.38 | 6.92 | 4.27 | -0.73 |
Golgi | 0.76 | 7.80 | 1.76 | 0.66 | -3.79 | -1.69 | 12.96 | 2.03 | 2.76 | -1.57 | -0.03 | 11.66 | 2.21 | 1.79 | -4.23 |
Lipid Particles | -0.86 | -2.46 | 1.18 | 1.61 | 4.75 | 4.37 | 1.50 | 4.41 | -0.75 | 3.51 | 3.02 | -0.54 | 1.81 | -1.30 | 3.29 |
Mitochondria | 4.42 | 6.66 | 4.22 | -0.55 | -2.65 | -3.02 | 7.35 | 5.88 | 4.41 | -1.28 | 2.35 | 7.25 | 4.15 | 1.80 | -2.68 |
Mitotic Spindle | 0.27 | -2.08 | -3.79 | -3.86 | -0.71 | -1.30 | -2.45 | -2.17 | -1.68 | 0.67 | -1.77 | -2.98 | -4.25 | -3.11 | 0.40 |
None | 4.54 | 4.44 | 4.41 | -3.98 | -0.70 | 2.78 | 2.10 | 5.17 | 1.42 | 4.11 | 4.59 | 4.51 | 4.90 | 1.13 | 3.67 |
Nuclear Periphery | -1.36 | -9.14 | -5.14 | 0.41 | 7.14 | 1.55 | -7.06 | -1.04 | -2.45 | 5.62 | 0.67 | -11.63 | -3.89 | -3.89 | 8.85 |
Nuclear Periphery Foci | -1.34 | -7.71 | -5.12 | -2.27 | 7.15 | 1.91 | -5.49 | -2.43 | -3.56 | 3.60 | 1.54 | -9.24 | -4.99 | -5.63 | 7.80 |
Nucleolus | 0.62 | 3.12 | 3.21 | 1.70 | 1.37 | -0.59 | 4.04 | 4.80 | 2.02 | 3.44 | -0.05 | 4.34 | 4.64 | 2.02 | 2.47 |
Nucleus | 1.63 | -2.92 | -0.96 | -2.25 | 2.50 | 8.70 | -4.95 | 2.28 | -4.57 | 6.29 | 10.26 | -5.93 | 0.65 | -10.25 | 6.42 |
Peroxisomes | -2.57 | 2.95 | 1.09 | 2.78 | -1.64 | -0.68 | -1.26 | -0.96 | -0.52 | 0.72 | -0.10 | -0.39 | 0.08 | 0.15 | 0.41 |
Vacuole | -3.17 | -11.33 | -16.43 | -3.79 | 5.55 | 9.83 | -11.39 | -7.63 | -11.66 | 5.43 | 7.60 | -16.12 | -17.42 | -20.77 | 6.77 |
Vacuole Periphery | -0.15 | 7.02 | 0.87 | 0.73 | -2.79 | -0.23 | 9.96 | 0.35 | 0.42 | -1.12 | 0.04 | 11.25 | 0.76 | 0.49 | -2.69 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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