Standard name
Human Ortholog
Description Kynurenine 3-monooxygenase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.08 0 0 0 0 0 0 0 0 0 0.05 0 0 – 0 0 0.11 0.07 0.08 0.1 0.09 0.12
Bud 0.07 0.09 0.08 0.11 0.09 0.05 0 0 0.05 0.05 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.19 0.27 0.14 0.18 0.38 0.33 0.43 0.5 0.52 0.68 0.59 0.61 0.35 0.55 0.82 – 0.86 0.87 0 0 0 0 0.12 0.12
Endoplasmic Reticulum 0 0.12 0 0 0 0 0 0 0 0 0 0 0 0.06 0 – 0.07 0 0 0 0 0 0 0.05
Endosome 0.15 0 0 0 0 0 0 0 0 0 0 0 0.14 0.1 0.05 – 0.06 0 0.16 0.18 0.17 0.12 0.08 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.07 0 – 0 0 0.13 0.08 0 0.09 0.05 0
Mitochondria 0.63 0.76 0.83 0.78 0.54 0.71 0.64 0.51 0.6 0.53 0.6 0.57 0.38 0.22 0.05 – 0.1 0.07 0.33 0.44 0.4 0.44 0.43 0.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0.17 0 0 0.1 0.05 0 0 0 0 0 0 0 0.13 0 0 – 0 0 0.14 0.05 0.15 0.09 0.05 0
SpindlePole 0.07 0 0 0.05 0 0 0.05 0.08 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.05 – 0 0 0 0.07 0 0 0.05 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 3 9 3 0 1 0 0 0 0 0 0 12 8 1 – 0 2 37 9 10 20 28 39
Bud 22 9 9 17 15 11 9 12 12 11 11 9 6 13 4 – 0 1 8 4 1 1 6 10
Bud Neck 0 0 0 0 0 0 1 3 2 1 3 1 0 0 0 – 0 0 3 1 3 5 4 11
Bud Site 2 0 2 2 2 3 1 4 7 1 0 2 0 0 1 – 0 0 – – – – – –
Cell Periphery 3 1 0 2 1 1 2 0 1 2 0 0 5 7 7 – 3 1 0 0 0 0 0 2
Cytoplasm 60 28 16 28 62 75 137 185 122 152 128 192 82 174 317 – 86 58 6 4 2 6 35 40
Endoplasmic Reticulum 2 12 5 2 3 7 0 1 0 0 0 1 5 18 17 – 7 1 4 0 4 5 12 14
Endosome 45 1 3 4 4 6 1 1 1 0 0 0 33 32 20 – 6 3 56 25 21 23 23 19
Golgi 1 2 0 1 1 0 1 0 0 0 0 0 23 22 7 – 0 0 46 11 4 16 15 13
Mitochondria 195 78 95 122 88 160 203 189 141 118 130 178 88 70 20 – 10 5 114 62 51 86 131 128
Nucleus 2 0 0 0 0 1 2 2 0 1 1 1 0 0 0 – 0 0 0 0 0 0 1 1
Nuclear Periphery 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 – 0 0 2 0 0 0 1 0
Nucleolus 3 0 0 0 0 0 1 4 0 0 0 1 1 0 1 – 0 1 0 0 0 0 1 0
Peroxisomes 52 1 4 16 8 5 0 0 0 0 0 0 30 10 2 – 0 0 49 7 20 18 16 6
SpindlePole 22 0 3 8 5 4 15 29 6 4 4 8 3 3 3 – 3 0 4 2 2 2 4 4
Vac/Vac Membrane 17 2 1 3 7 5 3 9 2 3 2 1 15 19 18 – 2 3 10 9 3 4 14 23
Unique Cell Count 309 102 114 156 162 224 315 369 236 224 217 314 232 315 388 100 67 351 142 130 194 303 323
Labelled Cell Count 428 137 147 208 197 279 376 439 294 293 279 394 303 376 418 117 75 351 142 130 194 303 323


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 4.8 4.2 4.2 4.2 3.4 2.8 3.1 3.0 2.7 2.8 2.5 6.7 5.7 5.6 4.4 5.8 5.4 5.4 5.3 5.8
Std Deviation (1e-4) 1.6 1.3 0.8 0.9 1.7 1.0 0.9 1.1 1.3 1.1 1.1 1.1 2.2 1.8 1.8 1.6 3.9 1.1 1.6 1.6 1.9
Intensity Change (Log2) – – – 0.0 0.0 -0.31 -0.58 -0.45 -0.49 -0.65 -0.59 -0.76 0.67 0.45 0.43 0.08 0.48 0.37 0.36 0.35 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6144 3.0181 2.8013 2.5372 2.2395 2.5167 1.4697 2.5063 1.8148 1.9803 2.2446 2.1605 3.519 3.7165 3.5863 4.632 4.4271 4.3612
Actin 0.1229 0.0815 0.1258 0.038 0.0282 0.0565 0.1449 0.0971 0.118 0.1003 0.0292 0.095 0.1044 0.1144 0.0992 0.0725 0.0802 0.1257
Bud 0.0022 0.0041 0.0064 0.0008 0.001 0.0006 0.002 0.005 0.004 0.0017 0.0004 0.001 0.0013 0.0059 0.0036 0.0025 0.001 0.0006
Bud Neck 0.0065 0.0043 0.0197 0.0152 0.0039 0.0386 0.0013 0.0031 0.0058 0.085 0.0028 0.0215 0.0034 0.0023 0.0121 0.0015 0.0042 0.0273
Bud Periphery 0.0024 0.0051 0.009 0.0008 0.0013 0.0007 0.0056 0.0084 0.004 0.0032 0.0003 0.0019 0.0017 0.0066 0.0067 0.0032 0.0023 0.001
Bud Site 0.0202 0.0796 0.0448 0.009 0.0023 0.0016 0.008 0.0629 0.0145 0.0085 0.0035 0.0014 0.0162 0.0677 0.0256 0.0043 0.002 0.0014
Cell Periphery 0.0008 0.0023 0.0026 0.0005 0.0002 0.0003 0.0011 0.0017 0.0007 0.0011 0.0001 0.0002 0.0011 0.0011 0.0013 0.0002 0.0002 0.0004
Cytoplasm 0.0254 0.1246 0.0598 0.0282 0.0095 0.0175 0.0337 0.0542 0.032 0.0217 0.0378 0.0153 0.0283 0.078 0.0505 0.024 0.0084 0.0277
Cytoplasmic Foci 0.217 0.1774 0.1759 0.1965 0.2923 0.2386 0.1665 0.1863 0.1776 0.1441 0.1456 0.1789 0.2 0.1806 0.1859 0.1607 0.2436 0.166
Eisosomes 0.0005 0.0005 0.0006 0.0002 0.0001 0.0002 0.0013 0.0005 0.0013 0.0003 0.0001 0.0003 0.0016 0.0005 0.0007 0.0002 0.0003 0.0004
Endoplasmic Reticulum 0.0018 0.006 0.0033 0.0008 0.0005 0.0019 0.0051 0.021 0.0021 0.0023 0.0008 0.001 0.0033 0.0011 0.0052 0.0019 0.0004 0.0009
Endosome 0.1291 0.1099 0.1101 0.1953 0.1449 0.1243 0.0985 0.0957 0.0867 0.0649 0.0494 0.0908 0.1376 0.0916 0.0809 0.1129 0.0526 0.0733
Golgi 0.1416 0.1344 0.1387 0.2424 0.1539 0.1736 0.0972 0.1068 0.1277 0.0708 0.1063 0.1734 0.1143 0.0861 0.1027 0.0884 0.1036 0.1423
Lipid Particles 0.0468 0.03 0.0353 0.0184 0.0299 0.0441 0.0573 0.0166 0.0159 0.0189 0.0783 0.0282 0.055 0.0112 0.0182 0.015 0.0169 0.0298
Mitochondria 0.0669 0.057 0.055 0.0342 0.0242 0.0578 0.0696 0.0376 0.0979 0.0787 0.0453 0.1147 0.0542 0.0266 0.0726 0.0986 0.0577 0.0498
None 0.0173 0.0293 0.0319 0.0466 0.0005 0.0173 0.0793 0.0932 0.0795 0.0038 0.0136 0.037 0.0308 0.0741 0.0322 0.0061 0.0059 0.0327
Nuclear Periphery 0.0009 0.0006 0.0007 0.0003 0.0001 0.0062 0.0043 0.002 0.0003 0.0005 0.0002 0.0014 0.0019 0.0002 0.0016 0.0005 0.0006 0.0002
Nucleolus 0.0004 0.0004 0.0011 0.0013 0.0001 0.0008 0.0013 0.0022 0.0003 0.0006 0.0002 0.0002 0.0004 0.0003 0.0005 0.0001 0.0003 0.0002
Nucleus 0.0006 0.0008 0.0011 0.0125 0.0001 0.0015 0.0078 0.0012 0.0003 0.0007 0.0001 0.001 0.0106 0.0005 0.0007 0.0003 0.0003 0.0002
Peroxisomes 0.1828 0.1451 0.1705 0.1484 0.3035 0.2122 0.1968 0.18 0.2231 0.3788 0.4832 0.2327 0.2285 0.2439 0.2937 0.4033 0.4034 0.3166
Punctate Nuclear 0.0019 0.0011 0.0012 0.0013 0.001 0.0024 0.0076 0.0036 0.001 0.0007 0.0006 0.0014 0.0021 0.001 0.0014 0.0009 0.0152 0.0011
Vacuole 0.0095 0.0047 0.0049 0.0071 0.0015 0.0019 0.0089 0.0184 0.0053 0.0105 0.0012 0.0015 0.0023 0.0053 0.0035 0.0018 0.0005 0.0017
Vacuole Periphery 0.0025 0.0013 0.0018 0.0021 0.0009 0.0015 0.002 0.0024 0.002 0.0027 0.0009 0.0012 0.0012 0.0009 0.0012 0.001 0.0003 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.7762 9.2718 7.9385 10.1783 7.7582 5.5817 12.2592 11.6812 7.6218 8.5682
Translational Efficiency 0.7285 1.1604 1.4459 1.114 1.0442 1.1333 1.1688 1.0681 1.5372 1.0024

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
310 971 1914 1547 1463 1465 231 327 1773 2436 2145 1874

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 693.64 760.19 1320.52 893.17 750.98 820.61 1251.78 927.34 740.95 796.53 1313.12 899.13
Standard Deviation 87.73 133.91 241.01 130.33 97.99 138.92 307.29 131.53 98.71 140.11 249.90 131.18
Intensity Change Log 2 — 0.132173 0.928847 0.364748 — 0.127922 0.737135 0.304324 — 0.129965 0.832369 0.333653

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.015534 0.056897 0.299658* 0.300657* 0.011978 0.048142 0.214642* 0.081678 0.012599 0.051631 0.290503* 0.262447*
Bud Neck 0.020847 0.008680 0.006312 0.009796 0.020193 0.005758 0.010896 0.014065 0.020307 0.006923 0.006806 0.010541
Bud Site 0.018917 0.033203 0.016984 0.105399* 0.033661 0.035554 0.049744 0.071854 0.031083 0.034617 0.020512 0.099546
Cell Periphery 0.000583 0.000770 0.000438 0.000563 0.000349 0.000629 0.000555 0.000455 0.000390 0.000685 0.000451 0.000544
Cytoplasm 0.211519* 0.022431 0.003606 0.115948* 0.258165* 0.022599 0.020188 0.100547* 0.250010* 0.022532 0.005392 0.113260*
Cytoplasmic Foci 0.346465* 0.156990 0.076306 0.103961 0.341420* 0.148018 0.119136 0.182817* 0.342302* 0.151594 0.080919 0.117721
Eisosomes 0.000836 0.000515 0.001015 0.000633 0.000491 0.000377 0.000624 0.000428 0.000551 0.000432 0.000973 0.000598
Endoplasmic Reticulum 0.001334 0.000897 0.002499 0.002370 0.001315 0.000399 0.002380 0.001940 0.001318 0.000597 0.002486 0.002295
Endosome 0.028475 0.146019 0.091085 0.037750 0.039172 0.137112 0.136861 0.125042 0.037302 0.140662 0.096015 0.052982
Golgi 0.086769 0.216167* 0.169488* 0.044928 0.059966 0.237225* 0.142245* 0.043979 0.064652 0.228831* 0.166554* 0.044762
Lipid Particles 0.021597 0.017471 0.080437 0.005650 0.016342 0.007025 0.071187 0.042504 0.017261 0.011189 0.079441 0.012080
Mitochondria 0.061818 0.128584* 0.123485* 0.013445 0.028861 0.148995* 0.025542 0.005535 0.034624 0.140859* 0.112938* 0.012065
Mitotic Spindle 0.004298 0.006449 0.007909 0.015216 0.006487 0.010161 0.031947 0.016398 0.006104 0.008681 0.010498 0.015422
None 0.002289 0.000212 0.000259 0.000100 0.003265 0.000211 0.000225 0.000607 0.003094 0.000212 0.000255 0.000189
Nuclear Periphery 0.000148 0.000106 0.000491 0.000083 0.000201 0.000037 0.000500 0.000371 0.000192 0.000065 0.000492 0.000133
Nuclear Periphery Foci 0.000330 0.000459 0.000751 0.000550 0.000571 0.000144 0.002012 0.001534 0.000529 0.000269 0.000887 0.000722
Nucleolus 0.000607 0.000344 0.000085 0.000062 0.000956 0.000123 0.000202 0.000590 0.000895 0.000211 0.000098 0.000154
Nucleus 0.047249 0.000931 0.000138 0.005515 0.045655 0.001313 0.003136 0.009259 0.045934 0.001161 0.000461 0.006168
Peroxisomes 0.117491* 0.185271* 0.108238* 0.192904* 0.101284* 0.181080* 0.119496* 0.188307* 0.104118* 0.182750* 0.109450* 0.192102*
Vacuole 0.010938 0.009876 0.006693 0.042575 0.026859 0.009623 0.040868 0.110316 0.024075 0.009724 0.010373 0.054395
Vacuole Periphery 0.001956 0.007728 0.004120 0.001896 0.002810 0.005475 0.007615 0.001772 0.002660 0.006373 0.004496 0.001874

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.89 -33.73* -28.63* -23.16* 1.53 -10.14 -9.87 -5.69 -2.11 6.07 -12.93 -38.50* -30.61* -24.29 4.28
Bud Neck 3.83 4.87 3.50 -0.85 -3.52 8.71 4.46 2.14 -2.86 -0.91 9.03 9.66 5.95 -3.32 -3.82
Bud Site -3.52 0.52 -16.68 -13.72 -19.53 -0.63 -2.13 -4.25 -3.96 -1.74 -1.37 4.06 -15.73 -14.84 -18.88
Cell Periphery -1.35 1.35 0.24 1.97 -2.16 -2.48 -2.06 -1.79 1.02 0.48 -3.57 -1.65 -3.01 1.64 -1.93
Cytoplasm 12.92 14.38 5.48 -18.25 -23.37 31.37 25.45 10.72 -7.98 -7.09 34.14 37.45 15.50 -20.38 -24.64
Cytoplasmic Foci 13.86 20.57 18.18 9.31 -6.23 28.07* 20.80 14.99 -2.73 -4.43 31.49 44.38 35.58 8.07 -8.37
Eisosomes 2.18 -1.40 1.54 -1.23 6.14 1.66 -1.24 1.11 -0.17 2.14 1.84 -6.62 -0.50 -2.71 6.90
Endoplasmic Reticulum 1.32 -2.60 -2.99 -4.50 -0.50 6.46 -3.62 -1.57 -3.23 0.74 5.07 -3.65 -3.87 -6.17 -0.31
Endosome -17.81 -13.46 -1.77 18.56 15.15 -20.56 -8.33 -6.39 2.43 1.49 -26.84 -17.20 -3.50 21.26 11.95
Golgi -11.14 -8.02 4.55 23.48 24.42* -26.92* -5.74 3.46 25.00 7.01 -30.33* -19.23 5.92 37.27 25.55*
Lipid Particles 1.11 -12.70 5.70 4.19 19.35 6.38 -5.17 -3.38 -4.89 2.59 3.86 -16.73 3.54 -0.18 17.96
Mitochondria -6.27 -6.61 5.96 16.02 21.59* -18.81 0.69 9.47 23.41 3.30 -20.74 -15.59 8.89 27.24 21.83*
Mitotic Spindle -1.01 -1.69 -4.04 -4.23 -3.57 -2.44 -3.94 -2.33 -1.44 2.17 -2.18 -3.23 -4.80 -3.65 -2.46
None 3.51 3.44 3.69 2.80 5.57 5.31 5.27 4.37 -1.88 -1.77 5.93 5.85 5.97 0.41 1.35
Nuclear Periphery 0.93 -3.61 0.85 -0.32 4.04 7.59 -3.53 -1.21 -2.27 0.71 5.49 -3.65 0.69 -2.90 3.76
Nuclear Periphery Foci -0.83 -2.47 -1.58 -1.00 0.24 4.15 -3.34 -3.51 -4.80 0.38 2.87 -2.15 -1.81 -3.51 -0.04
Nucleolus 1.45 4.40 4.48 2.10 0.66 6.04 5.03 1.23 -2.46 -2.00 5.44 6.99 6.01 0.53 -1.96
Nucleus 8.36 8.52 7.02 -8.36 -9.64 16.52 11.35 10.61 -5.91 -2.08 18.60 18.78 14.85 -9.60 -10.16
Peroxisomes -4.98 0.71 -5.10 0.38 -9.73 -9.81 -1.08 -4.43 1.10 -2.63 -11.66 -0.93 -10.43 0.57 -10.02
Vacuole 0.61 2.78 -13.06 -13.73 -14.96 9.57 -3.26 -9.78 -11.06 -5.82 9.77 6.40 -13.56 -17.42 -16.23
Vacuole Periphery -4.57 -4.89 0.32 4.68 5.10 -4.37 -1.72 2.40 5.43 2.15 -5.93 -4.19 2.44 6.94 5.56
Standard name
Human Ortholog
Description Kynurenine 3-monooxygenase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Bna4

Bna4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bna4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available