Standard name
Human Ortholog
Description Protein that associates with mitochondrial ribosome; likely functions in cristae junction formation; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud 0 0 0 0 0.08 0 0 – 0 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.06 0 0 0 0 – 0 0 0 0 0 0 0 0.11 0.13 0.08 0 0 0 0 0 0
Cytoplasm 0.36 0.22 0.28 0.21 0.15 0.11 0.09 – 0.1 0.07 0.08 0.05 0.48 0.51 0.63 0.22 0.09 0.09 0.07 0 0 0 0 0
Endoplasmic Reticulum 0 0.05 0 0 0.08 0 0 – 0 0 0 0 0 0 0 0.05 0 0.07 0.1 0 0 0 0 0
Endosome 0 0 0.13 0.12 0 0.05 0.15 – 0 0 0 0 0.16 0.16 0.14 0.09 0.09 0.09 0.11 0.08 0.07 0.07 0.05 0.06
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.1 0.36 0.2 0 0 0 0 0 0
Mitochondria 0.38 0.7 0.26 0.62 0.73 0.74 0.72 – 0.87 0.9 0.91 0.89 0.16 0.1 0.08 0.13 0.15 0.13 0.45 0.69 0.7 0.66 0.7 0.65
Nucleus 0.1 0 0 0 0 0 0 – 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.07 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.08 0.08 0 0 0.05 – 0 0 0 0 0 0.16 0.12 0.11 0.07 0.08 0 0 0.05 0 0 0
SpindlePole 0 0 0.19 0.05 0.05 0.05 0.1 – 0 0.07 0 0.05 0.07 0.08 0 0.07 0.07 0.11 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.13 0.11 0 0 0.08 0.15 – 0.06 0.05 0.05 0.06 0.1 0.12 0.09 0.16 0.15 0.24 0.12 0.08 0 0.06 0.06 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 4 2 2 1 0 – 0 0 1 0 5 3 2 4 8 6 8 9 8 9 9 30
Bud 1 7 5 6 10 7 5 – 8 11 3 8 3 7 1 11 11 10 2 3 0 5 9 10
Bud Neck 4 0 5 1 3 0 1 – 1 0 1 0 1 0 1 1 0 0 3 4 1 3 4 8
Bud Site 1 0 1 0 2 2 2 – 3 3 6 6 0 0 0 0 1 1 – – – – – –
Cell Periphery 1 8 9 3 1 1 0 – 0 1 4 1 5 7 5 26 30 22 0 3 1 1 0 1
Cytoplasm 56 81 45 36 19 25 21 – 25 15 14 10 126 207 299 54 20 24 21 12 8 12 21 7
Endoplasmic Reticulum 4 20 2 5 10 6 1 – 3 0 1 3 6 3 2 13 4 19 29 5 3 4 4 5
Endosome 4 9 20 20 0 11 33 – 0 1 0 4 43 66 65 22 20 23 34 41 18 25 22 37
Golgi 1 1 1 1 0 1 1 – 0 0 0 0 6 1 3 25 81 53 9 11 0 5 6 25
Mitochondria 59 260 41 105 93 164 160 – 220 193 150 184 43 39 39 32 34 35 136 337 187 236 332 391
Nucleus 16 5 2 0 5 5 5 – 10 11 3 12 2 4 1 7 8 10 0 0 0 2 3 3
Nuclear Periphery 11 0 0 0 0 1 0 – 2 3 4 2 0 0 0 6 1 2 2 0 2 2 3 4
Nucleolus 1 0 1 0 0 1 1 – 5 3 1 0 0 0 0 1 1 3 1 2 0 3 1 4
Peroxisomes 0 3 12 13 0 1 11 – 1 6 0 2 10 66 57 28 17 21 1 6 13 10 9 22
SpindlePole 5 12 30 8 6 11 23 – 9 16 2 11 19 33 11 16 15 30 8 8 5 7 6 8
Vac/Vac Membrane 13 50 17 6 3 17 33 – 16 11 9 13 27 47 44 39 35 63 35 39 8 22 28 24
Unique Cell Count 156 374 158 170 127 221 223 254 215 165 207 264 403 472 246 228 265 304 492 267 359 472 600
Labelled Cell Count 177 458 195 206 154 254 297 303 274 199 256 296 483 530 285 286 322 304 492 267 359 472 600


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 4.1 5.3 5.4 4.3 4.6 5.2 – 4.0 4.4 4.0 4.5 6.1 6.0 5.7 5.9 7.3 7.3 6.5 6.8 6.9
Std Deviation (1e-4) 0.6 0.7 1.2 1.8 0.9 1.3 2.1 – 1.3 1.7 1.1 1.4 1.5 1.6 1.8 1.1 1.4 1.4 1.8 2.2 2.1
Intensity Change (Log2) – – – 0.02 -0.32 -0.21 -0.04 – -0.41 -0.27 -0.4 -0.25 0.19 0.17 0.09 0.14 0.45 0.44 0.28 0.35 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 1.4 1.3 3.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5148 0.5304 0.4617 0.1082 0.4639 0.5606 5.5345 5.2848 5.6253 5.2132 5.1472 5.6614 2.6792 2.5534 2.6894 2.4297 2.9438 2.6228
Actin 0.058 0.0154 0.0156 0.0029 0.0159 0.0067 0.0325 0.0207 0.0204 0.0064 0.016 0.0097 0.0197 0.0106 0.0265 0.0045 0.0173 0.009
Bud 0.0014 0.0038 0.0022 0.0038 0.0017 0.0093 0.0022 0.0077 0.0052 0.0044 0.0067 0.0019 0.0017 0.0037 0.0041 0.0039 0.0032 0.0017
Bud Neck 0.0033 0.003 0.0077 0.0012 0.0014 0.0113 0.0045 0.002 0.0039 0.0008 0.0011 0.005 0.0053 0.0016 0.0108 0.0009 0.0032 0.0041
Bud Periphery 0.0042 0.0056 0.0048 0.0154 0.0043 0.0072 0.0062 0.0107 0.0146 0.01 0.0133 0.0051 0.004 0.0085 0.011 0.0096 0.0082 0.01
Bud Site 0.005 0.0322 0.022 0.0019 0.002 0.003 0.013 0.0436 0.0192 0.0027 0.0038 0.0039 0.0082 0.0344 0.0458 0.0035 0.0051 0.0029
Cell Periphery 0.002 0.0021 0.0022 0.0014 0.0005 0.0012 0.0081 0.0022 0.0022 0.001 0.001 0.0024 0.002 0.0024 0.0092 0.0011 0.0028 0.0025
Cytoplasm 0.0219 0.0357 0.0436 0.051 0.0136 0.0169 0.0319 0.0531 0.0564 0.0506 0.0386 0.0463 0.0417 0.0665 0.0519 0.0738 0.0498 0.0271
Cytoplasmic Foci 0.0644 0.0776 0.0599 0.0501 0.0626 0.0484 0.0785 0.0963 0.0935 0.0346 0.1004 0.0766 0.0821 0.0887 0.0942 0.0507 0.0856 0.083
Eisosomes 0.0013 0.0008 0.0017 0.0003 0.0005 0.0003 0.0014 0.0005 0.0008 0.0003 0.0002 0.0004 0.0011 0.0011 0.0015 0.0007 0.0004 0.001
Endoplasmic Reticulum 0.0139 0.0051 0.0031 0.0049 0.0059 0.0035 0.014 0.0067 0.0045 0.002 0.0021 0.0027 0.0104 0.0046 0.008 0.0053 0.0043 0.0032
Endosome 0.1542 0.1601 0.0984 0.1306 0.2261 0.132 0.153 0.1592 0.1153 0.1006 0.1387 0.1405 0.229 0.1844 0.1237 0.1288 0.1864 0.089
Golgi 0.0557 0.0711 0.0676 0.0647 0.0469 0.069 0.0728 0.0711 0.047 0.0574 0.0881 0.0814 0.0797 0.0697 0.0535 0.0381 0.0785 0.0516
Lipid Particles 0.0664 0.0361 0.0141 0.0109 0.0205 0.0271 0.0243 0.0137 0.0251 0.016 0.0074 0.0214 0.0565 0.018 0.0171 0.0044 0.0155 0.0195
Mitochondria 0.408 0.4163 0.5364 0.5185 0.3984 0.6172 0.4178 0.3941 0.4324 0.6106 0.4532 0.4898 0.2873 0.3281 0.375 0.5157 0.3972 0.5594
None 0.067 0.0739 0.0649 0.0956 0.0923 0.0114 0.0628 0.0485 0.0907 0.06 0.048 0.0516 0.0864 0.1004 0.1012 0.1153 0.0679 0.0548
Nuclear Periphery 0.0107 0.0031 0.0024 0.0019 0.0214 0.0012 0.0085 0.0016 0.0009 0.0009 0.0026 0.0008 0.0109 0.0024 0.0028 0.004 0.0099 0.0021
Nucleolus 0.0011 0.0023 0.0023 0.001 0.0013 0.0011 0.0011 0.0012 0.0019 0.0003 0.0002 0.0008 0.0041 0.0037 0.0015 0.0008 0.0012 0.0006
Nucleus 0.0042 0.0021 0.0028 0.0014 0.0086 0.0016 0.0039 0.0017 0.0019 0.0008 0.0012 0.0015 0.0126 0.005 0.0019 0.0027 0.0061 0.0014
Peroxisomes 0.0286 0.0286 0.0234 0.0234 0.0569 0.0143 0.0388 0.0395 0.0465 0.0225 0.0503 0.0406 0.0291 0.0325 0.0358 0.0123 0.0319 0.0584
Punctate Nuclear 0.0082 0.0017 0.0026 0.0013 0.0048 0.0004 0.005 0.0008 0.0015 0.0006 0.0004 0.0008 0.003 0.0018 0.0049 0.0014 0.0036 0.0024
Vacuole 0.0139 0.0167 0.0163 0.0126 0.0089 0.0108 0.0127 0.019 0.0114 0.0098 0.0162 0.0113 0.0187 0.0249 0.0136 0.0144 0.0144 0.0107
Vacuole Periphery 0.0065 0.0067 0.0059 0.0052 0.0055 0.0061 0.007 0.0062 0.0048 0.0077 0.0103 0.0057 0.0064 0.0072 0.0063 0.0081 0.0075 0.0054

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.9292 19.4121 23.4075 15.6074 18.4765 20.3467 25.4545 19.4569 29.8208 21.8631
Translational Efficiency 0.1634 0.153 0.1852 0.3338 0.1989 0.183 0.1885 0.253 0.2543 0.2688

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
188 1157 3002 1749 1407 2161 1595 1899 1595 3318 4597 3648

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 647.93 723.53 839.66 803.31 656.74 798.40 837.69 809.41 655.70 772.29 838.98 806.49
Standard Deviation 72.33 122.15 100.66 126.85 78.12 88.78 96.08 107.09 77.51 107.75 99.10 117.02
Intensity Change Log 2 — 0.159215 0.373967 0.310119 — 0.281789 0.351094 0.301548 — 0.222217 0.362499 0.305811

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000989 0.002422 0.002758 0.001126 0.000515 0.002079 0.001900 0.005352 0.000571 0.002199 0.002460 0.003326
Bud Neck 0.024001 0.034595 0.061636 0.028397 0.033659 0.038851 0.073104 0.047192 0.032521 0.037367 0.065615 0.038181
Bud Site 0.019119 0.039691 0.053867 0.045401 0.018451 0.031890 0.098353 0.090768 0.018530 0.034610 0.069302 0.069017
Cell Periphery 0.001836 0.000525 0.000355 0.000211 0.000272 0.000337 0.000134 0.000254 0.000457 0.000402 0.000278 0.000234
Cytoplasm 0.019504 0.052851 0.110792* 0.359077* 0.121167* 0.062731 0.157111* 0.343103* 0.109184* 0.059286 0.126863* 0.350761*
Cytoplasmic Foci 0.085592 0.170802 0.091677 0.085907 0.325134* 0.191184* 0.086051 0.120233 0.296900* 0.184076* 0.089725 0.103776
Eisosomes 0.000723 0.000249 0.000132 0.000091 0.000202 0.000295 0.000082 0.000144 0.000263 0.000279 0.000115 0.000119
Endoplasmic Reticulum 0.002750 0.000941 0.004004 0.003021 0.001475 0.000496 0.001897 0.003633 0.001626 0.000651 0.003273 0.003339
Endosome 0.030891 0.094210 0.058586 0.022740 0.058351 0.061700 0.046989 0.018707 0.055114 0.073036 0.054562 0.020640
Golgi 0.062993 0.106349 0.021222 0.004146 0.055307 0.083350 0.020924 0.009248 0.056213 0.091370 0.021119 0.006802
Lipid Particles 0.007446 0.009927 0.009859 0.005336 0.019271 0.007739 0.001845 0.003791 0.017877 0.008502 0.007079 0.004532
Mitochondria 0.629418* 0.326051* 0.164185* 0.009828 0.213531* 0.390573* 0.161191* 0.021808 0.262551* 0.368074* 0.163146* 0.016065
Mitotic Spindle 0.006624 0.017717 0.083802 0.064590 0.010120 0.011077 0.086477 0.034029 0.009708 0.013392 0.084730 0.048682
None 0.000566 0.006301 0.002929 0.003704 0.003817 0.000842 0.000843 0.002185 0.003434 0.002746 0.002205 0.002913
Nuclear Periphery 0.000493 0.000369 0.004114 0.002781 0.000545 0.000092 0.001654 0.000806 0.000539 0.000189 0.003261 0.001753
Nuclear Periphery Foci 0.000818 0.000433 0.001040 0.002760 0.000474 0.000100 0.000468 0.001010 0.000515 0.000216 0.000842 0.001849
Nucleolus 0.001155 0.004713 0.008275 0.002221 0.004368 0.000966 0.003075 0.001521 0.003990 0.002273 0.006471 0.001857
Nucleus 0.015847 0.016283 0.096482 0.150660* 0.043575 0.008377 0.045080 0.122999 0.040307 0.011134 0.078647 0.136260*
Peroxisomes 0.072739 0.059878 0.028677 0.013615 0.038274 0.072840 0.037193 0.039895 0.042336 0.068320 0.031632 0.027295
Vacuole 0.007975 0.039465 0.176951 0.189727 0.038746 0.027499 0.162719 0.130224 0.035119 0.031672 0.172013 0.158752
Vacuole Periphery 0.008522 0.016230 0.018656 0.004661 0.012745 0.006982 0.012912 0.003097 0.012247 0.010207 0.016663 0.003847

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.47 -4.98 0.13 2.65 5.92 -4.69 -7.72 -6.95 -3.75 -4.55 -5.57 -8.99 -7.25 -1.55 -1.20
Bud Neck -1.77 -6.49 0.07 3.90 15.27 -1.86 -11.08 -3.31 -1.48 8.24 -2.03 -13.18 -0.59 1.77 15.01
Bud Site -3.63 -6.08 -2.28 2.26 6.50 -6.07 -18.74 -16.23 -11.87 3.01 -8.14 -20.38 -14.75 -7.98 3.84
Cell Periphery 2.48 2.85 3.21 4.14 2.82 -1.14 3.93 1.14 2.37 -5.94 0.66 2.35 3.70 4.85 1.70
Cytoplasm -6.16 -17.66 -40.32 -38.15 -30.27 10.85 -6.40 -27.56 -39.79 -20.43 10.51 -4.70 -36.86 -55.03 -37.94
Cytoplasmic Foci -8.11 -0.58 1.54 17.50 5.74 18.41 36.01 31.13 15.01 -5.97 17.15 34.72 33.43 23.66 -1.08
Eisosomes 2.05 2.58 2.78 5.62 4.27 -3.88 8.55 4.35 7.68 -7.19 -0.47 4.91 5.06 10.00 0.31
Endoplasmic Reticulum 1.01 -0.78 -0.09 -8.23 4.15 5.32 -2.52 -4.57 -6.66 -3.39 3.72 -6.28 -4.74 -9.65 0.27
Endosome -10.74 -5.30 3.22 19.55 19.28 -1.03 4.40 15.35 18.69 12.23 -5.86 1.12 15.94 26.81 23.12
Golgi -4.39 5.00 7.05 22.69 14.72 -7.22 11.86 16.66 26.77 8.02 -9.84 13.10 19.01 35.43 15.38
Lipid Particles -1.39 -1.35 1.73 4.54 6.59 8.29 13.71 12.15 5.86 -5.58 7.45 9.09 11.96 7.11 5.74
Mitochondria 11.34 18.51 24.61 33.70* 36.11* -17.37 7.12 25.83 50.85* 23.12* -10.85 12.74 31.32 61.28* 41.68*
Mitotic Spindle -4.00 -19.58* -10.54 -8.24 6.95 -0.55 -16.45 -7.97 -7.95 10.49 -2.45 -25.81 -12.81 -11.87 12.79
None -3.64 -4.37 -4.00 1.92 -0.32 4.74 4.98 2.44 -4.43 -5.63 0.93 2.14 1.31 0.14 -1.14
Nuclear Periphery 0.37 -7.53 -6.96 -11.01 2.33 4.50 -2.69 -4.30 -17.70 1.64 3.39 -9.12 -10.18 -15.39 4.13
Nuclear Periphery Foci 0.50 -0.36 -1.63 -3.47 -2.26 3.37 -0.17 -3.50 -5.35 -3.16 2.23 -2.34 -4.23 -5.67 -3.23
Nucleolus -3.41 -8.79 -1.65 2.90 10.16 6.04 1.88 4.96 -2.63 2.98 2.97 -3.81 4.44 1.17 10.68
Nucleus -0.13 -10.82 -18.13 -29.53 -11.98 12.33 -2.52 -19.19 -31.11 -16.54 10.77 -12.36 -27.87 -43.46 -17.38
Peroxisomes 1.09 3.95 5.59 13.90 10.51 -9.71 0.98 1.40 10.86 0.41 -8.17 4.62 7.80 18.01 4.88
Vacuole -10.65 -41.33 -40.09 -33.54 -10.08 4.24 -24.93 -25.67 -29.35 2.35 1.35 -40.29 -41.30 -44.52 -5.18
Vacuole Periphery -3.78 -4.74 3.25 12.03 16.91 8.02 0.84 14.59 11.57 9.57 2.72 -3.90 14.69 15.86 19.94*
Standard name
Human Ortholog
Description Protein that associates with mitochondrial ribosome; likely functions in cristae junction formation; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
Localization
Cell Percentages mitochondrion (64%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Mrx3

Mrx3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrx3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available