ORF | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 46 | 35 | 12 | 22 | 30 | 12 | 19 | 26 | 24 | 37 | 17 | 90 | 145 | 65 | 105 | 102 | 88 | 15 | 68 | 69 | 52 | 83 | 61 |
Bud | – | 11 | 11 | 12 | 11 | 20 | 20 | 27 | 25 | 34 | 18 | 16 | 19 | 27 | 17 | 25 | 5 | 14 | 0 | 1 | 0 | 2 | 2 | 6 |
Bud Neck | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 33 | 14 | 14 | 21 | 22 |
Bud Site | – | 1 | 6 | 3 | 1 | 2 | 4 | 8 | 6 | 9 | 1 | 2 | 1 | 1 | 0 | 2 | 2 | 1 | – | – | – | – | – | – |
Cell Periphery | – | 40 | 14 | 20 | 18 | 20 | 22 | 35 | 38 | 45 | 29 | 31 | 94 | 194 | 240 | 160 | 174 | 105 | 5 | 46 | 38 | 55 | 113 | 104 |
Cytoplasm | – | 67 | 48 | 55 | 46 | 33 | 50 | 73 | 52 | 42 | 33 | 24 | 91 | 108 | 137 | 63 | 30 | 24 | 1 | 10 | 2 | 14 | 26 | 33 |
Endoplasmic Reticulum | – | 17 | 5 | 3 | 11 | 29 | 22 | 16 | 15 | 15 | 16 | 12 | 12 | 20 | 17 | 12 | 17 | 11 | 3 | 12 | 7 | 19 | 46 | 38 |
Endosome | – | 0 | 2 | 2 | 0 | 0 | 3 | 9 | 0 | 0 | 0 | 0 | 4 | 2 | 24 | 3 | 0 | 0 | 1 | 5 | 4 | 0 | 0 | 2 |
Golgi | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 29 | 17 | 0 | 3 | 1 | 0 | 0 | 1 |
Mitochondria | – | 15 | 30 | 31 | 22 | 93 | 164 | 158 | 161 | 208 | 145 | 166 | 6 | 14 | 18 | 33 | 29 | 17 | 1 | 8 | 13 | 13 | 21 | 19 |
Nucleus | – | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 1 | 3 | 1 | 0 | 1 | 0 | 5 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 0 | 11 | 8 | 11 | 11 | 18 | 27 | 12 | 14 | 7 | 7 | 17 | 71 | 112 | 12 | 9 | 3 | 0 | 1 | 1 | 0 | 0 | 1 |
SpindlePole | – | 0 | 5 | 7 | 2 | 0 | 4 | 8 | 3 | 5 | 1 | 2 | 6 | 4 | 16 | 10 | 4 | 6 | 0 | 0 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | – | 0 | 0 | 1 | 0 | 1 | 1 | 3 | 1 | 2 | 1 | 1 | 0 | 0 | 6 | 4 | 4 | 0 | 0 | 5 | 1 | 4 | 8 | 11 |
Unique Cell Count | 151 | 120 | 109 | 111 | 166 | 244 | 281 | 233 | 262 | 187 | 202 | 226 | 393 | 416 | 296 | 269 | 197 | 39 | 199 | 157 | 179 | 332 | 314 | |
Labelled Cell Count | 197 | 167 | 155 | 145 | 239 | 323 | 386 | 341 | 401 | 292 | 280 | 340 | 588 | 652 | 441 | 408 | 287 | 39 | 199 | 157 | 179 | 332 | 314 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 5.5 | 4.7 | 5.0 | 5.6 | 4.6 | 4.3 | 4.8 | 4.8 | 4.2 | 4.7 | 4.3 | 5.8 | 6.1 | 6.3 | 8.1 | 10.5 | 10.8 | 5.1 | 5.3 | 6.2 |
Std Deviation (1e-4) | – | 0.9 | 1.4 | 1.7 | 2.1 | 1.1 | 1.2 | 1.4 | 1.6 | 1.1 | 1.7 | 1.1 | 1.0 | 1.2 | 1.2 | 1.4 | 1.8 | 1.9 | 1.4 | 1.5 | 1.8 |
Intensity Change (Log2) | – | – | – | 0.06 | 0.23 | -0.03 | -0.16 | 0.02 | 0.01 | -0.17 | -0.01 | -0.14 | 0.3 | 0.36 | 0.41 | 0.77 | 1.15 | 1.19 | 0.12 | 0.15 | 0.39 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -3.4 | -1.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.0 | 1.6 | -3.4 | 1.2 | 1.7 | 2.7 |
Bud | 0.5 | 0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.2 | -0.8 | -2.2 | -0.2 | 0 | -0.7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.4 | 1.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5.7 | 7.4 | 8.9 | 7.9 | 9.7 | 7.4 |
Cytoplasm | 1.6 | 0.2 | -3.7 | -3.9 | -2.8 | -3.5 | -5.1 | -4.3 | -5.9 | 0 | -2.6 | -1.4 | -3.9 | -6.6 | -5.7 |
Endoplasmic Reticulum | 0 | 1.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.5 | 0.4 | 0 | 0 | 0.8 | 0.6 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.8 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 3.3 |
Mitochondria | 0.6 | -0.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -6.5 | -7.3 | -7.0 | -3.6 | -3.6 | -4.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -0.5 | 0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.5 | 2.3 | 4.1 | -2.1 | -2.4 | -3.2 |
SpindlePole | 0.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.5186 | 1.5688 | 0.6855 | 0.9765 | 0.3887 | 0.868 | 2.6159 | 2.9458 | 3.1111 | 2.7793 | 2.6452 | 2.7153 | 3.9691 | 4.6583 | 4.5778 | 4.149 | 3.242 | 3.4531 |
Actin | 0.1989 | 0.1505 | 0.1625 | 0.2608 | 0.2264 | 0.2129 | 0.1504 | 0.089 | 0.0939 | 0.0815 | 0.2725 | 0.1538 | 0.1622 | 0.0751 | 0.1197 | 0.1364 | 0.2088 | 0.1877 |
Bud | 0.0029 | 0.002 | 0.0024 | 0.0027 | 0.0018 | 0.0026 | 0.0015 | 0.0013 | 0.0014 | 0.0019 | 0.0034 | 0.0014 | 0.005 | 0.0079 | 0.0015 | 0.0235 | 0.0014 | 0.0138 |
Bud Neck | 0.0303 | 0.0369 | 0.1798 | 0.3117 | 0.0822 | 0.1854 | 0.0291 | 0.0207 | 0.1656 | 0.2659 | 0.0936 | 0.1471 | 0.0288 | 0.0298 | 0.0997 | 0.2229 | 0.1411 | 0.0999 |
Bud Periphery | 0.008 | 0.0054 | 0.014 | 0.016 | 0.011 | 0.0207 | 0.0077 | 0.006 | 0.0076 | 0.0057 | 0.0109 | 0.018 | 0.0095 | 0.0098 | 0.0081 | 0.0363 | 0.0116 | 0.0131 |
Bud Site | 0.1008 | 0.369 | 0.1318 | 0.0466 | 0.0543 | 0.0123 | 0.1307 | 0.4232 | 0.1924 | 0.055 | 0.0187 | 0.0093 | 0.1061 | 0.3392 | 0.105 | 0.0518 | 0.0177 | 0.0066 |
Cell Periphery | 0.1017 | 0.0778 | 0.097 | 0.0759 | 0.1614 | 0.1242 | 0.2797 | 0.132 | 0.1513 | 0.1192 | 0.2492 | 0.2303 | 0.1933 | 0.0781 | 0.1496 | 0.1731 | 0.1714 | 0.1288 |
Cytoplasm | 0.1746 | 0.2031 | 0.154 | 0.1943 | 0.2079 | 0.238 | 0.1723 | 0.2577 | 0.1503 | 0.1424 | 0.1796 | 0.3342 | 0.241 | 0.2541 | 0.2862 | 0.1949 | 0.3062 | 0.3689 |
Cytoplasmic Foci | 0.0537 | 0.0211 | 0.0296 | 0.0101 | 0.0416 | 0.0359 | 0.0088 | 0.0054 | 0.0335 | 0.0552 | 0.0266 | 0.0089 | 0.0223 | 0.0131 | 0.0134 | 0.0051 | 0.017 | 0.0184 |
Eisosomes | 0.0326 | 0.0107 | 0.0178 | 0.0149 | 0.0605 | 0.0136 | 0.0312 | 0.0087 | 0.0272 | 0.0047 | 0.0249 | 0.0214 | 0.0381 | 0.0106 | 0.0133 | 0.0089 | 0.0159 | 0.0185 |
Endoplasmic Reticulum | 0.0142 | 0.0103 | 0.0105 | 0.0036 | 0.0066 | 0.0063 | 0.004 | 0.0042 | 0.0031 | 0.0047 | 0.0047 | 0.0027 | 0.0162 | 0.0037 | 0.0051 | 0.0026 | 0.0058 | 0.0049 |
Endosome | 0.0142 | 0.0028 | 0.0062 | 0.0019 | 0.0016 | 0.0045 | 0.0062 | 0.0004 | 0.0091 | 0.0482 | 0.0223 | 0.0006 | 0.0147 | 0.0047 | 0.0029 | 0.0006 | 0.0072 | 0.001 |
Golgi | 0.0171 | 0.0088 | 0.0136 | 0.0059 | 0.0048 | 0.0074 | 0.0058 | 0.0025 | 0.0126 | 0.0319 | 0.054 | 0.0024 | 0.0092 | 0.0031 | 0.0097 | 0.0023 | 0.0194 | 0.0048 |
Lipid Particles | 0.0264 | 0.0078 | 0.0063 | 0.0018 | 0.004 | 0.0087 | 0.0043 | 0.002 | 0.0063 | 0.042 | 0.008 | 0.001 | 0.0096 | 0.0022 | 0.0036 | 0.001 | 0.0151 | 0.0033 |
Mitochondria | 0.0113 | 0.0051 | 0.0061 | 0.0022 | 0.0538 | 0.0052 | 0.0177 | 0.0001 | 0.0102 | 0.0086 | 0.018 | 0.0004 | 0.0087 | 0.0089 | 0.0101 | 0.0245 | 0.0082 | 0.0022 |
None | 0.1783 | 0.0817 | 0.1549 | 0.0468 | 0.0749 | 0.1061 | 0.1424 | 0.0434 | 0.1072 | 0.1 | 0.0021 | 0.0661 | 0.1008 | 0.1521 | 0.1643 | 0.1047 | 0.0429 | 0.1205 |
Nuclear Periphery | 0.0029 | 0.0006 | 0.0007 | 0.0003 | 0.0003 | 0.0004 | 0.0009 | 0.0003 | 0.0012 | 0.0033 | 0.0015 | 0.0001 | 0.0126 | 0.0003 | 0.0003 | 0.0004 | 0.0007 | 0.0002 |
Nucleolus | 0.0024 | 0.0005 | 0.0011 | 0.0004 | 0.0003 | 0.0007 | 0.0007 | 0.0005 | 0.001 | 0.0008 | 0.0002 | 0.0001 | 0.0014 | 0.0005 | 0.0006 | 0.0019 | 0.0003 | 0.0005 |
Nucleus | 0.0027 | 0.0006 | 0.0013 | 0.0007 | 0.0004 | 0.0011 | 0.0005 | 0.0006 | 0.0018 | 0.0011 | 0.0005 | 0.0002 | 0.0031 | 0.0009 | 0.0007 | 0.0042 | 0.0004 | 0.0011 |
Peroxisomes | 0.0168 | 0.0019 | 0.0045 | 0.0014 | 0.004 | 0.0104 | 0.0024 | 0.0002 | 0.0189 | 0.0194 | 0.0063 | 0.0009 | 0.0034 | 0.0035 | 0.0037 | 0.0013 | 0.006 | 0.0035 |
Punctate Nuclear | 0.0058 | 0.0017 | 0.0034 | 0.0006 | 0.0009 | 0.0015 | 0.0006 | 0.0003 | 0.0041 | 0.0046 | 0.0006 | 0.0001 | 0.0056 | 0.0003 | 0.0004 | 0.0004 | 0.0006 | 0.0004 |
Vacuole | 0.0037 | 0.0014 | 0.0021 | 0.0012 | 0.0008 | 0.002 | 0.0022 | 0.0017 | 0.0012 | 0.0028 | 0.0016 | 0.0009 | 0.0043 | 0.0019 | 0.0019 | 0.0025 | 0.0019 | 0.0018 |
Vacuole Periphery | 0.0007 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0008 | 0 | 0.0002 | 0.0011 | 0.0007 | 0.0001 | 0.004 | 0.0003 | 0.0001 | 0.001 | 0.0004 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.6324 | 3.5203 | 5.7468 | 9.5876 | 9.7008 | 12.5944 | 7.1187 | 8.4081 | 9.9065 | 12.3941 |
Translational Efficiency | 1.1483 | 2.2309 | 0.9993 | 0.9279 | 1.2569 | 1.2801 | 1.0779 | 1.0147 | 1.068 | 0.8599 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1475 | 1457 | 1808 | 1518 | 687 | 1680 | 118 | 251 | 2162 | 3137 | 1926 | 1769 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 700.07 | 776.60 | 979.54 | 974.97 | 749.89 | 739.55 | 1057.04 | 1003.40 | 715.90 | 756.76 | 984.29 | 979.00 |
Standard Deviation | 75.61 | 98.17 | 112.74 | 135.20 | 84.85 | 99.82 | 111.67 | 146.77 | 82.01 | 100.77 | 114.20 | 137.26 |
Intensity Change Log 2 | — | 0.149673 | 0.484605 | 0.477859 | — | -0.020031 | 0.495279 | 0.420146 | — | 0.064399 | 0.490135 | 0.448299 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.045022 | 0.082539 | 0.168836* | 0.310557* | 0.026402 | 0.063978 | 0.251661* | 0.126382* | 0.039105 | 0.072598 | 0.173910* | 0.284425* |
Bud Neck | 0.106026 | 0.067724 | 0.086864 | 0.042068 | 0.085605 | 0.095840 | 0.035378 | 0.030658 | 0.099537 | 0.082781 | 0.083710 | 0.040449 |
Bud Site | 0.035956 | 0.067312 | 0.168618* | 0.159069* | 0.038198 | 0.054544 | 0.160481* | 0.112121* | 0.036668 | 0.060474 | 0.168120* | 0.152408* |
Cell Periphery | 0.088620 | 0.026201 | 0.107270* | 0.080956 | 0.031474 | 0.029017 | 0.131089* | 0.056293 | 0.070461 | 0.027709 | 0.108730* | 0.077456 |
Cytoplasm | 0.382742* | 0.322169* | 0.179519* | 0.168155* | 0.445355* | 0.346869* | 0.091800 | 0.222981* | 0.402638* | 0.335397* | 0.174145* | 0.175934* |
Cytoplasmic Foci | 0.047513 | 0.152641 | 0.020175 | 0.014727 | 0.147146 | 0.105090 | 0.053350 | 0.101028 | 0.079173 | 0.127175 | 0.022208 | 0.026972 |
Eisosomes | 0.139288* | 0.046639 | 0.076151 | 0.081185 | 0.038091 | 0.071214 | 0.063019 | 0.046837 | 0.107132* | 0.059800 | 0.075346 | 0.076312 |
Endoplasmic Reticulum | 0.005878 | 0.014729 | 0.078789 | 0.012160 | 0.008610 | 0.010409 | 0.056511 | 0.011869 | 0.006746 | 0.012415 | 0.077424 | 0.012118 |
Endosome | 0.003070 | 0.017947 | 0.004530 | 0.004559 | 0.009332 | 0.015911 | 0.007235 | 0.012663 | 0.005060 | 0.016857 | 0.004696 | 0.005709 |
Golgi | 0.019054 | 0.052399 | 0.006762 | 0.004917 | 0.027315 | 0.049681 | 0.008194 | 0.004821 | 0.021679 | 0.050943 | 0.006850 | 0.004904 |
Lipid Particles | 0.024015 | 0.027605 | 0.015367 | 0.005741 | 0.029925 | 0.032783 | 0.013054 | 0.033746 | 0.025893 | 0.030378 | 0.015225 | 0.009715 |
Mitochondria | 0.011268 | 0.017693 | 0.001558 | 0.002067 | 0.007394 | 0.025548 | 0.000157 | 0.001152 | 0.010037 | 0.021900 | 0.001472 | 0.001938 |
Mitotic Spindle | 0.000206 | 0.000559 | 0.000311 | 0.003123 | 0.000119 | 0.000690 | 0.000209 | 0.006452 | 0.000178 | 0.000629 | 0.000304 | 0.003596 |
None | 0.001843 | 0.000406 | 0.000605 | 0.000147 | 0.001690 | 0.000804 | 0.000049 | 0.000144 | 0.001795 | 0.000619 | 0.000571 | 0.000147 |
Nuclear Periphery | 0.000099 | 0.000105 | 0.000173 | 0.000066 | 0.000229 | 0.000099 | 0.000168 | 0.000306 | 0.000140 | 0.000102 | 0.000172 | 0.000100 |
Nuclear Periphery Foci | 0.000275 | 0.000426 | 0.002983 | 0.000919 | 0.000639 | 0.000339 | 0.006778 | 0.002922 | 0.000391 | 0.000379 | 0.003215 | 0.001203 |
Nucleolus | 0.000119 | 0.000144 | 0.000068 | 0.000041 | 0.000214 | 0.000150 | 0.000058 | 0.000117 | 0.000149 | 0.000147 | 0.000067 | 0.000051 |
Nucleus | 0.024580 | 0.018985 | 0.037314 | 0.020118 | 0.048946 | 0.020098 | 0.024972 | 0.022143 | 0.032323 | 0.019581 | 0.036557 | 0.020405 |
Peroxisomes | 0.057466 | 0.054209 | 0.008249 | 0.006753 | 0.031404 | 0.055632 | 0.005295 | 0.018388 | 0.049184 | 0.054971 | 0.008068 | 0.008404 |
Vacuole | 0.006668 | 0.027718 | 0.035644 | 0.081667 | 0.021172 | 0.019471 | 0.090428 | 0.188419 | 0.011277 | 0.023302 | 0.039001 | 0.096814 |
Vacuole Periphery | 0.000293 | 0.001853 | 0.000213 | 0.001003 | 0.000741 | 0.001833 | 0.000112 | 0.000559 | 0.000435 | 0.001842 | 0.000207 | 0.000940 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -7.96 | -22.82 | -34.04* | -26.75* | -15.48 | -8.97 | -9.09 | -7.38 | -4.30 | 4.84 | -10.60 | -26.09 | -34.76* | -28.94* | -12.62 |
Bud Neck | 7.00 | 3.57 | 13.45 | 7.23 | 12.10 | -1.51 | 6.03 | 8.79 | 13.42 | 0.90 | 3.85 | 3.48 | 15.40 | 14.23 | 12.53 |
Bud Site | -8.79 | -25.86* | -22.31 | -15.71 | 1.62 | -4.07 | -7.36 | -6.51 | -5.08 | 2.59 | -9.14 | -27.30 | -23.31 | -18.27 | 2.71 |
Cell Periphery | 15.58 | -3.75 | 2.28 | -13.68 | 6.29 | 0.72 | -6.77 | -2.40 | -2.79 | 4.80 | 14.57 | -9.12 | -0.89 | -14.15 | 7.78 |
Cytoplasm | 7.11 | 27.01 | 26.35 | 19.56 | 1.20 | 9.34 | 25.75 | 13.21 | 8.11 | -7.22 | 10.26 | 34.96 | 31.86 | 24.95 | -0.72 |
Cytoplasmic Foci | -20.74 | 10.44 | 12.43 | 29.61 | 3.24 | 6.28 | 8.81 | 4.41 | 0.51 | -3.63 | -12.48 | 19.49 | 16.36 | 30.47 | -2.27 |
Eisosomes | 18.98 | 12.38 | 11.43 | -8.27 | -0.56 | -8.71 | -2.93 | -0.76 | 4.48 | 2.00 | 12.70 | 7.78 | 7.95 | -3.95 | 0.49 |
Endoplasmic Reticulum | -5.74 | -20.96 | -4.90 | 1.36 | 18.52 | -1.13 | -4.64 | -1.30 | -0.47 | 4.33 | -5.38 | -21.30 | -4.68 | 0.19 | 19.12 |
Endosome | -13.64 | -3.10 | -1.66 | 10.36 | 0.14 | -5.52 | 1.25 | -1.42 | 1.60 | -2.09 | -15.75 | 0.71 | -0.62 | 11.45 | -1.03 |
Golgi | -13.47 | 10.75 | 9.94 | 19.56 | 1.88 | -8.16 | 7.35 | 10.92 | 20.61 | 1.82 | -16.58 | 14.37 | 13.40 | 26.97 | 2.26 |
Lipid Particles | -1.57 | 4.87 | 11.18 | 12.86 | 9.89 | -1.01 | 5.73 | -0.36 | 0.13 | -3.23 | -2.53 | 7.00 | 10.39 | 13.81 | 4.70 |
Mitochondria | -4.57 | 11.29 | 9.31 | 12.65 | -0.79 | -9.89 | 6.76 | 5.11 | 15.12 | -1.45 | -10.15 | 12.44 | 10.04 | 18.83 | -0.80 |
Mitotic Spindle | -1.37 | -2.14 | -3.59 | -2.99 | -3.46 | -2.68 | -2.37 | -1.88 | -1.70 | -1.85 | -2.72 | -3.40 | -4.04 | -3.41 | -3.88 |
None | 3.85 | 2.84 | 4.57 | 5.41 | 2.08 | 1.85 | 3.65 | 3.39 | 3.64 | -1.87 | 3.85 | 3.40 | 5.66 | 4.99 | 2.04 |
Nuclear Periphery | -0.30 | -7.32 | 2.44 | 1.58 | 8.49 | 1.68 | 0.68 | -0.56 | -1.61 | -1.04 | 1.41 | -1.37 | 1.11 | -0.10 | 3.22 |
Nuclear Periphery Foci | -1.94 | -14.43 | -6.25 | -5.58 | 10.00 | 2.93 | -4.09 | -3.13 | -3.52 | 2.29 | 0.28 | -14.21 | -6.04 | -6.40 | 8.27 |
Nucleolus | -1.62 | 4.03 | 4.62 | 6.91 | 2.15 | 2.53 | 6.41 | 2.58 | 1.02 | -2.18 | 0.10 | 7.06 | 6.26 | 7.47 | 1.28 |
Nucleus | 3.68 | -6.25 | 2.26 | -1.43 | 8.13 | 10.36 | 5.26 | 7.64 | -0.78 | 0.74 | 9.41 | -2.36 | 7.36 | -1.37 | 8.18 |
Peroxisomes | 0.95 | 17.79 | 18.16 | 21.81 | 2.01 | -7.57 | 9.87 | 3.57 | 10.06 | -3.48 | -2.31 | 19.80 | 19.11 | 28.32 | -0.09 |
Vacuole | -13.78 | -17.62 | -20.27 | -14.70 | -12.48 | 0.85 | -5.53 | -11.72 | -11.84 | -5.80 | -11.05 | -15.60 | -22.19 | -19.31 | -14.77 |
Vacuole Periphery | -8.33 | 1.64 | -1.90 | 2.23 | -2.11 | -4.92 | 6.28 | 0.57 | 3.11 | -1.09 | -9.93 | 4.21 | -1.51 | 2.80 | -2.24 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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