Standard name
Human Ortholog
Description Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Cytoplasm 0.91 0.94 0.94 0.94 0.8 0.63 0.66 0.46 0.42 0.43 0.32 0.28 0.99 0.98 0.98 0.24 0.15 0.11 0.56 0.67 0.54 0.76 0.73 0.74
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.21 0.22 0.2 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Mitochondria 0.16 0 0.06 0.13 0.2 0.44 0.42 0.57 0.66 0.62 0.73 0.74 0 0 0 0.16 0.17 0.13 0.19 0.19 0.26 0.13 0.11 0.09
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.14 0.2 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0.05 0.09 0.05 0 0.06 0 0 0 0 0.24 0.29 0.26 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.14 0.19 0.06 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 3 4 8 2 4 2
Bud 3 3 6 4 5 11 11 20 14 9 4 3 0 0 0 18 6 10 2 2 2 0 10 8
Bud Neck 2 1 8 1 10 6 5 7 0 3 3 0 0 0 0 4 1 1 0 2 1 0 2 5
Bud Site 0 0 1 0 1 1 4 8 4 2 1 2 0 0 0 4 10 5 – – – – – –
Cell Periphery 2 2 0 0 0 1 2 0 2 6 1 4 0 1 0 26 12 22 0 2 4 0 4 2
Cytoplasm 152 277 226 172 207 159 237 123 86 107 49 39 266 362 421 152 63 35 119 212 122 135 227 199
Endoplasmic Reticulum 0 2 3 0 1 1 0 0 0 1 4 0 1 2 1 33 22 14 6 0 1 1 0 1
Endosome 2 1 0 0 1 0 0 0 0 0 0 0 1 2 3 133 91 62 7 5 4 2 1 3
Golgi 0 2 0 0 0 0 0 0 0 0 0 0 0 0 1 14 9 14 1 0 0 0 1 1
Mitochondria 26 12 15 24 51 112 152 154 134 153 112 102 0 4 2 100 70 41 41 58 59 22 34 25
Nucleus 0 1 0 3 1 0 2 1 0 1 0 2 1 2 1 15 17 6 0 0 1 0 0 0
Nuclear Periphery 1 0 0 1 0 1 0 1 0 2 3 1 0 1 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 2 1 0 3 2 1 0 0 0 0 0 3 1 3 0 0 0 0 0 0
Peroxisomes 0 1 0 0 1 0 0 2 1 0 0 0 0 0 0 102 59 62 0 0 0 0 0 0
SpindlePole 0 0 1 1 10 7 18 24 10 8 9 4 0 1 1 150 119 79 6 5 4 3 12 1
Vac/Vac Membrane 0 4 0 0 0 1 1 3 3 2 0 6 1 0 2 76 59 58 13 10 5 2 4 13
Unique Cell Count 167 295 240 183 258 252 359 268 204 248 154 137 268 370 429 622 407 307 212 315 226 178 313 271
Labelled Cell Count 188 306 261 206 290 301 432 346 256 295 186 163 270 375 432 831 540 412 212 315 226 178 313 271


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.1 3.6 2.8 2.7 3.1 2.6 2.4 2.8 2.8 2.5 2.6 2.9 4.0 3.8 3.8 5.3 6.2 6.2 3.5 3.8 4.2
Std Deviation (1e-4) 0.7 0.7 0.5 1.0 1.2 0.7 1.1 1.1 1.2 0.9 0.9 1.4 1.6 1.3 1.2 1.4 1.4 1.5 1.0 1.4 1.2
Intensity Change (Log2) – – – -0.07 0.15 -0.15 -0.25 -0.02 -0.03 -0.19 -0.13 0.04 0.5 0.42 0.43 0.89 1.13 1.13 0.31 0.41 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 5.7 6.2 7.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.1495 0.0066 0.0012 0.0026 0.0006 0.0435 0.0427 0.0142 0.011 0.0094 0.0076 0.0061 0 0.0057 0.0002 0.0094 0.0005 0.0002
Bud 0.0012 0.0004 0.0004 0.0159 0.0071 0.0006 0.0005 0.0005 0.0011 0.0143 0.0005 0.0019 0 0.0007 0 0.0009 0.0001 0
Bud Neck 0.008 0.0002 0.0001 0.0003 0.0001 0.0027 0.0006 0.0005 0.0038 0.0022 0.0002 0.0005 0 0.0004 0.0002 0.0009 0 0.0001
Bud Periphery 0.0038 0.0008 0.0035 0.0559 0.0252 0.0024 0.0015 0.0006 0.0017 0.0054 0.0026 0.0023 0 0.0013 0 0.0022 0.0002 0
Bud Site 0.0133 0.0006 0.0007 0.0078 0.0003 0.0003 0.0018 0.0106 0.0092 0.0332 0.0004 0.0013 0 0.0125 0.0003 0.0007 0 0
Cell Periphery 0.014 0.0014 0.0025 0.0021 0.0006 0.0005 0.0006 0.0006 0.0007 0.0008 0.0001 0.0001 0 0.0013 0.0002 0.0008 0.0001 0.0001
Cytoplasm 0.1795 0.1943 0.1561 0.3252 0.1168 0.1363 0.0209 0.0631 0.0602 0.0498 0.0408 0.0769 0.0121 0.1361 0.0424 0.0536 0.042 0.0786
Cytoplasmic Foci 0.0836 0.0115 0.007 0.0179 0.0182 0.0439 0.0687 0.0192 0.0427 0.0407 0.06 0.0368 0.0008 0.0111 0.0077 0.0182 0.0061 0.0324
Eisosomes 0.0119 0.0012 0.0014 0.0002 0.0005 0.0005 0.0005 0.0006 0.0005 0.0005 0.0006 0.0001 0.0001 0.0004 0.0003 0.0002 0.0002 0.0001
Endoplasmic Reticulum 0.0773 0.0033 0.0026 0.0056 0.0102 0.0117 0.0018 0.0017 0.0015 0.0023 0.0011 0.001 0.0001 0.0012 0.0012 0.0012 0.0014 0.0018
Endosome 0.0498 0.007 0.0019 0.0182 0.015 0.0155 0.0484 0.0044 0.0362 0.0126 0.0124 0.0097 0 0.0034 0.0024 0.0387 0.0193 0.0154
Golgi 0.0346 0.0075 0.0006 0.0051 0.0038 0.0051 0.0153 0.005 0.0226 0.0073 0.0039 0.0045 0 0.0079 0.0004 0.0353 0.0073 0.0008
Lipid Particles 0.0665 0.0016 0.001 0.0005 0.0028 0.0041 0.0276 0.0019 0.0065 0.0152 0.018 0.0036 0 0.0003 0.0011 0.014 0.0035 0.008
Mitochondria 0.0057 0.0037 0.002 0.123 0.0917 0.0536 0.0119 0.003 0.0092 0.0068 0.0028 0.002 0 0.003 0.0002 0.0835 0.1571 0.0004
None 0.2698 0.7574 0.8157 0.4013 0.6951 0.648 0.6696 0.8645 0.7756 0.7596 0.7832 0.8423 0.9865 0.8132 0.9416 0.7196 0.7575 0.8589
Nuclear Periphery 0.0064 0.0003 0.0003 0.0018 0.0006 0.0012 0.005 0.0004 0.0006 0.0027 0.0007 0.0003 0 0.0002 0.0002 0.0004 0.0004 0.0004
Nucleolus 0.0017 0.0002 0.0005 0.0005 0.0002 0.0002 0.0013 0.0005 0.0004 0.004 0.0003 0.0001 0 0.0001 0.0001 0.0006 0.0001 0.0001
Nucleus 0.0026 0.0003 0.0005 0.0043 0.0006 0.0005 0.0019 0.0005 0.0007 0.0063 0.0003 0.0008 0 0.0002 0.0002 0.0009 0.0001 0.0001
Peroxisomes 0.0067 0.0005 0.0005 0.0013 0.0014 0.0239 0.0432 0.0047 0.0105 0.0164 0.0616 0.0075 0 0.0003 0.0001 0.0045 0.0007 0.0007
Punctate Nuclear 0.0062 0.0002 0.0003 0.0005 0.0002 0.0027 0.0192 0.0018 0.001 0.0071 0.0019 0.0014 0 0.0001 0.0002 0.0002 0.0001 0.001
Vacuole 0.0065 0.0008 0.0009 0.0067 0.005 0.0014 0.0124 0.0015 0.0036 0.0026 0.0006 0.0006 0 0.0004 0.0006 0.011 0.0013 0.0006
Vacuole Periphery 0.0014 0.0002 0.0001 0.0033 0.0039 0.0012 0.0045 0.0002 0.0008 0.0009 0.0003 0.0001 0 0.0001 0.0001 0.0032 0.002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.7502 6.0663 10.8034 7.9561 9.2383 8.1387 10.7338 12.0053 10.8046 10.9316
Translational Efficiency 1.0373 0.8096 0.3896 0.5288 0.3655 0.7603 0.6316 0.5896 0.5608 0.4814

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1347 1501 1437 1959 1598 246 222 2514 2945 1747 1659 4473

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1541.78 1682.63 1951.52 2692.55 1750.66 1976.19 2492.81 2763.29 1655.12 1723.97 2023.95 2732.31
Standard Deviation 321.78 500.70 541.23 574.82 416.42 559.83 426.68 612.14 390.23 519.57 558.62 597.11
Intensity Change Log 2 — 0.126121 0.340001 0.804376 — 0.174823 0.509874 0.658488 — 0.152221 0.432809 0.728644

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000502 0.000504 0.000589 0.000630 0.000490 0.000607 0.000748 0.000509 0.000495 0.000518 0.000610 0.000562
Bud Neck 0.006385 0.004518 0.009988 0.018601 0.003505 0.004373 0.011318 0.019010 0.004822 0.004497 0.010166 0.018831
Bud Site 0.000550 0.000502 0.000853 0.001541 0.000393 0.000772 0.001095 0.002451 0.000465 0.000540 0.000885 0.002053
Cell Periphery 0.000061 0.000138 0.000039 0.000112 0.000037 0.000033 0.000043 0.000238 0.000048 0.000123 0.000040 0.000183
Cytoplasm 0.000218 0.001100 0.000804 0.000560 0.000178 0.000304 0.000055 0.000914 0.000196 0.000988 0.000704 0.000759
Cytoplasmic Foci 0.000080 0.000153 0.000259 0.000802 0.000457 0.001424 0.000303 0.001249 0.000284 0.000332 0.000265 0.001053
Eisosomes 0.000091 0.000110 0.000092 0.000051 0.000163 0.000097 0.000133 0.000062 0.000130 0.000108 0.000097 0.000057
Endoplasmic Reticulum 0.000756 0.000426 0.000231 0.000402 0.000364 0.000392 0.000294 0.000545 0.000543 0.000421 0.000239 0.000482
Endosome 0.000088 0.000028 0.000011 0.000573 0.000040 0.000176 0.000009 0.000492 0.000062 0.000049 0.000011 0.000528
Golgi 0.000066 0.000037 0.000052 0.000744 0.000083 0.000139 0.000059 0.000643 0.000075 0.000051 0.000053 0.000688
Lipid Particles 0.000019 0.000005 0.000005 0.000041 0.000053 0.000059 0.000006 0.000066 0.000037 0.000013 0.000005 0.000055
Mitochondria 0.001028 0.000963 0.001454 0.002496 0.000822 0.001180 0.001923 0.001722 0.000916 0.000994 0.001517 0.002061
Mitotic Spindle 0.001067 0.000077 0.000085 0.003702 0.000410 0.008692 0.000031 0.008529 0.000710 0.001290 0.000078 0.006415
None 0.001389 0.003055 0.000850 0.001003 0.000819 0.002212 0.000738 0.001256 0.001080 0.002936 0.000835 0.001145
Nuclear Periphery 0.000047 0.000050 0.000045 0.000341 0.000020 0.000142 0.000040 0.000276 0.000033 0.000063 0.000045 0.000305
Nuclear Periphery Foci 0.000055 0.000045 0.000012 0.000080 0.000063 0.000330 0.000002 0.000072 0.000060 0.000085 0.000010 0.000076
Nucleolus 0.058002 0.050637 0.048664 0.016444 0.062376 0.069899 0.033426 0.015801 0.060375 0.053349 0.046625 0.016083
Nucleus 0.927376* 0.934823* 0.932281* 0.950057* 0.926138* 0.901931* 0.945366* 0.944127* 0.926704* 0.930191* 0.934032* 0.946724*
Peroxisomes 0.001391 0.001871 0.003303 0.000782 0.002764 0.006414 0.003960 0.000456 0.002136 0.002511 0.003391 0.000599
Vacuole 0.000450 0.000514 0.000192 0.000574 0.000455 0.000534 0.000303 0.001053 0.000453 0.000517 0.000207 0.000843
Vacuole Periphery 0.000378 0.000444 0.000192 0.000465 0.000371 0.000290 0.000146 0.000530 0.000374 0.000422 0.000186 0.000501

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.18 -5.74 -7.41 -6.97 -3.48 -3.07 -7.43 -4.53 -0.38 4.58 -1.29 -8.02 -8.20 -6.56 -0.87
Bud Neck 4.49 -8.76 -18.11 -20.22 -13.24 -1.59 -9.88 -22.22 -15.74 -6.67 0.66 -15.80 -28.53 -27.73 -17.02
Bud Site 0.41 -6.13 -5.99 -6.05 -4.57 -1.02 -5.70 -7.39 -2.30 -3.78 -1.06 -8.27 -9.44 -8.10 -7.06
Cell Periphery -1.38 1.65 -2.58 0.44 -4.38 0.21 -1.02 -3.50 -3.51 -3.28 -1.59 1.00 -4.10 -0.83 -4.42
Cytoplasm -1.79 -0.95 -2.44 0.51 -0.05 -1.01 1.55 -5.63 -3.80 -7.38 -1.89 -0.96 -5.35 0.13 -0.44
Cytoplasmic Foci -1.99 -5.65 -3.05 -2.76 -2.17 -1.01 0.20 -1.41 0.67 -1.87 -0.53 -0.17 -2.67 -1.66 -3.01
Eisosomes -1.28 -1.12 6.42 3.69 8.21 5.03 0.84 8.75 4.84 8.55 1.59 3.70 10.47 3.90 11.63
Endoplasmic Reticulum 3.41 9.29 5.04 -0.53 -13.09 -0.53 0.10 -4.06 -2.57 -4.08 1.63 9.72 0.05 -1.58 -9.06
Endosome 1.33 1.72 -1.69 -2.04 -2.13 -1.25 1.45 -2.45 -0.79 -2.72 0.24 2.24 -2.89 -2.97 -3.41
Golgi 1.49 0.36 -1.81 -1.92 -1.84 -0.71 0.12 -1.43 -1.21 -1.47 0.84 0.54 -2.23 -2.36 -2.31
Lipid Particles 1.49 1.50 -1.30 -2.54 -2.54 -0.34 1.13 -0.16 0.21 -1.71 1.06 1.48 -0.52 -2.01 -2.56
Mitochondria 0.59 -4.47 -6.43 -7.14 -4.83 -2.75 -8.78 -8.69 -4.71 0.76 -1.22 -10.07 -10.09 -9.90 -5.21
Mitotic Spindle 1.31 1.22 -2.84 -4.18 -4.13 -1.59 1.62 -5.75 -0.23 -6.02 -0.70 1.58 -6.33 -5.05 -7.23
None -1.97 1.57 0.53 2.37 -4.22 -1.36 0.31 -3.46 0.71 -5.93 -2.63 1.29 -1.52 2.29 -6.41
Nuclear Periphery -0.16 -0.14 -4.52 -4.48 -4.51 -1.13 -4.69 -9.37 -2.24 -8.23 -1.65 -3.30 -8.41 -7.30 -8.03
Nuclear Periphery Foci 0.38 2.36 -1.15 -1.44 -2.99 -1.79 2.08 -0.39 1.73 -5.55 -0.73 2.66 -1.00 0.01 -5.30
Nucleolus 1.90 1.48 10.67 9.56 9.71 -1.03 5.10 13.09 6.85 3.47 2.17 3.33 16.93 12.66 11.43
Nucleus -1.66 0.14 -1.51 0.12 -1.74 2.42 -1.87 -1.14 -2.90 1.08 -0.77 -0.45 -1.82 -0.81 -1.23
Peroxisomes -2.39 -7.01 2.12 3.69 7.67 -3.89 -2.53 8.60 6.38 5.89 -1.56 -5.38 8.54 8.03 10.08
Vacuole -0.29 2.17 -1.56 -1.17 -3.28 -0.48 0.11 -1.26 -0.50 -1.52 -0.24 1.75 -1.74 -1.54 -3.23
Vacuole Periphery -0.53 2.66 -0.11 0.24 -1.20 0.45 1.78 -0.37 -0.75 -1.77 -0.40 2.53 -0.37 -0.02 -2.00
Standard name
Human Ortholog
Description Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Pet112

Pet112


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pet112-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available