Standard name | |
---|---|
Human Ortholog | |
Description | Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 3 | 2 | 3 | 8 |
Bud | 0 | 1 | 0 | 1 | 9 | 15 | 13 | 6 | 25 | 19 | 37 | 38 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 5 | 0 | 4 | 3 | 13 |
Bud Neck | 0 | 0 | 0 | 0 | 2 | 3 | 4 | 3 | 1 | 3 | 4 | 7 | 1 | 1 | 2 | 0 | 0 | 0 | 1 | 3 | 0 | 1 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 5 | 3 | 6 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 2 | 3 | 2 | 3 | 6 | 3 | 7 | 6 | 6 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Cytoplasm | 131 | 384 | 202 | 294 | 742 | 784 | 1010 | 833 | 637 | 815 | 668 | 860 | 225 | 595 | 630 | 87 | 75 | 62 | 140 | 378 | 201 | 250 | 349 | 378 |
Endoplasmic Reticulum | 0 | 2 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 3 | 3 | 3 | 0 | 4 | 5 | 0 | 0 | 1 | 2 | 2 | 2 | 8 |
Endosome | 3 | 5 | 0 | 6 | 0 | 1 | 2 | 4 | 1 | 0 | 2 | 1 | 1 | 0 | 3 | 3 | 4 | 2 | 2 | 9 | 2 | 1 | 4 | 14 |
Golgi | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 0 | 2 |
Mitochondria | 8 | 2 | 16 | 55 | 141 | 307 | 371 | 166 | 369 | 294 | 473 | 590 | 1 | 0 | 0 | 11 | 6 | 0 | 6 | 6 | 3 | 13 | 15 | 41 |
Nucleus | 3 | 7 | 2 | 3 | 1 | 5 | 4 | 9 | 5 | 2 | 4 | 6 | 8 | 5 | 5 | 17 | 8 | 6 | 8 | 31 | 6 | 16 | 14 | 1 |
Nuclear Periphery | 2 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 1 | 2 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 |
SpindlePole | 0 | 0 | 0 | 1 | 3 | 4 | 10 | 20 | 11 | 23 | 21 | 28 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 7 | 4 | 3 | 5 | 21 |
Vac/Vac Membrane | 18 | 68 | 11 | 55 | 12 | 3 | 2 | 3 | 13 | 5 | 5 | 2 | 18 | 47 | 22 | 21 | 37 | 26 | 30 | 29 | 7 | 53 | 37 | 22 |
Unique Cell Count | 156 | 443 | 220 | 351 | 799 | 913 | 1201 | 933 | 848 | 994 | 935 | 1221 | 243 | 629 | 650 | 117 | 117 | 91 | 202 | 489 | 241 | 363 | 445 | 527 |
Labelled Cell Count | 165 | 472 | 234 | 417 | 915 | 1127 | 1425 | 1052 | 1071 | 1173 | 1231 | 1551 | 259 | 652 | 664 | 145 | 137 | 96 | 202 | 489 | 241 | 363 | 445 | 527 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 5.7 | 4.2 | 4.1 | 3.4 | 3.1 | 2.8 | 3.2 | 2.8 | 2.7 | 2.6 | 2.6 | 5.9 | 5.6 | 5.4 | 5.8 | 6.2 | 7.0 | 6.4 | 6.1 | 5.8 |
Std Deviation (1e-4) | 0.9 | 1.0 | 0.8 | 1.2 | 1.3 | 1.5 | 1.3 | 1.4 | 1.2 | 0.8 | 1.1 | 0.6 | 1.1 | 1.0 | 1.0 | 1.0 | 1.1 | 1.9 | 1.2 | 1.4 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.02 | -0.31 | -0.42 | -0.6 | -0.38 | -0.58 | -0.63 | -0.69 | -0.71 | 0.49 | 0.42 | 0.37 | 0.48 | 0.56 | 0.73 | 0.62 | 0.55 | 0.46 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -2.8 | 0.5 | -2.4 | -3.0 | -1.1 | -5.4 | -3.6 | -6.3 | -6.6 | 0.3 | 1.5 | 3.2 | -4.4 | -6.3 | -5.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.9 | 0 | 0 | 0.7 | -0.8 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5.2 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 3.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.1 | 1.3 | -1.1 | 3.9 | 6.7 | 5.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.2726 | 1.6803 | 1.3504 | 1.0846 | 1.4316 | 1.445 | 1.6188 | 2.1293 | 2.0922 | 1.345 | 1.853 | 1.8934 | -0.8814 | 0.3071 | -0.1855 | 0.2681 | -0.1915 | 0.1784 |
Actin | 0.0346 | 0.0048 | 0.005 | 0.0075 | 0.0047 | 0.0063 | 0.0388 | 0.0008 | 0.0159 | 0.0017 | 0.0184 | 0.0016 | 0.0125 | 0.0003 | 0.0074 | 0.0198 | 0.0414 | 0.0024 |
Bud | 0.0055 | 0.0154 | 0.017 | 0.0094 | 0.0196 | 0.0054 | 0.0014 | 0.0007 | 0.0004 | 0.001 | 0.0005 | 0.0004 | 0.0002 | 0.0002 | 0.0004 | 0.0004 | 0.0006 | 0.0002 |
Bud Neck | 0.0058 | 0.0008 | 0.0024 | 0.0009 | 0.0073 | 0.0045 | 0.0026 | 0.0005 | 0.0011 | 0.0006 | 0.0012 | 0.0014 | 0.0032 | 0.0087 | 0.0006 | 0.0011 | 0.0021 | 0.0027 |
Bud Periphery | 0.0044 | 0.0041 | 0.0038 | 0.0129 | 0.0046 | 0.0041 | 0.002 | 0.0013 | 0.0003 | 0.0024 | 0.0006 | 0.0004 | 0.0002 | 0.0004 | 0.0006 | 0.0003 | 0.0005 | 0.0001 |
Bud Site | 0.0109 | 0.0279 | 0.019 | 0.008 | 0.0351 | 0.0031 | 0.0127 | 0.0056 | 0.0091 | 0.0007 | 0.0082 | 0.0004 | 0.0066 | 0.0024 | 0.0014 | 0.0013 | 0.0023 | 0.0002 |
Cell Periphery | 0.0007 | 0.0005 | 0.0008 | 0.0001 | 0.0006 | 0.0003 | 0.0004 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0 | 0.0001 | 0 |
Cytoplasm | 0.2426 | 0.4242 | 0.4183 | 0.4515 | 0.2754 | 0.5113 | 0.4169 | 0.6149 | 0.5822 | 0.5421 | 0.4411 | 0.5977 | 0.5127 | 0.6183 | 0.5853 | 0.4753 | 0.5576 | 0.7186 |
Cytoplasmic Foci | 0.0645 | 0.0253 | 0.0281 | 0.0562 | 0.039 | 0.0514 | 0.0258 | 0.0081 | 0.0162 | 0.0235 | 0.0268 | 0.014 | 0.0192 | 0.0096 | 0.01 | 0.0257 | 0.0209 | 0.0064 |
Eisosomes | 0.001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0004 | 0 |
Endoplasmic Reticulum | 0.0059 | 0.0031 | 0.0029 | 0.0015 | 0.004 | 0.0043 | 0.0074 | 0.003 | 0.0053 | 0.0056 | 0.0033 | 0.0044 | 0.0064 | 0.0037 | 0.0047 | 0.0072 | 0.0038 | 0.004 |
Endosome | 0.0508 | 0.0075 | 0.0203 | 0.0099 | 0.0225 | 0.0157 | 0.0244 | 0.0048 | 0.012 | 0.0114 | 0.0236 | 0.01 | 0.0124 | 0.0026 | 0.0145 | 0.0154 | 0.0135 | 0.0102 |
Golgi | 0.0177 | 0.0024 | 0.01 | 0.0096 | 0.0071 | 0.0061 | 0.0069 | 0.001 | 0.004 | 0.0039 | 0.0083 | 0.0024 | 0.0033 | 0.0002 | 0.0033 | 0.0045 | 0.0062 | 0.0007 |
Lipid Particles | 0.0297 | 0.0069 | 0.0104 | 0.0067 | 0.0896 | 0.0078 | 0.01 | 0.0013 | 0.0018 | 0.0125 | 0.0102 | 0.0021 | 0.0094 | 0.0005 | 0.007 | 0.0064 | 0.0227 | 0.0119 |
Mitochondria | 0.0192 | 0.0065 | 0.0179 | 0.0176 | 0.0104 | 0.0228 | 0.007 | 0.0014 | 0.0054 | 0.0072 | 0.0043 | 0.0079 | 0.0011 | 0.0002 | 0.0023 | 0.002 | 0.0029 | 0.0008 |
None | 0.3954 | 0.3463 | 0.3405 | 0.2218 | 0.3283 | 0.2357 | 0.3352 | 0.288 | 0.2747 | 0.2861 | 0.3969 | 0.2819 | 0.3812 | 0.2995 | 0.296 | 0.2478 | 0.1912 | 0.0962 |
Nuclear Periphery | 0.0159 | 0.005 | 0.0068 | 0.004 | 0.0051 | 0.0105 | 0.0116 | 0.0022 | 0.0057 | 0.0082 | 0.0045 | 0.0042 | 0.0044 | 0.0033 | 0.0053 | 0.0158 | 0.0062 | 0.0078 |
Nucleolus | 0.0026 | 0.0027 | 0.0035 | 0.0039 | 0.002 | 0.0035 | 0.0053 | 0.0023 | 0.0005 | 0.0005 | 0.0005 | 0.0003 | 0.0006 | 0.0003 | 0.0003 | 0.0006 | 0.0009 | 0.0006 |
Nucleus | 0.0225 | 0.0743 | 0.0524 | 0.0679 | 0.0186 | 0.0533 | 0.0504 | 0.0419 | 0.0541 | 0.0716 | 0.013 | 0.0525 | 0.0119 | 0.0435 | 0.0467 | 0.085 | 0.1034 | 0.1247 |
Peroxisomes | 0.0366 | 0.0066 | 0.0126 | 0.0583 | 0.0367 | 0.009 | 0.0224 | 0.0005 | 0.0027 | 0.0109 | 0.0254 | 0.0096 | 0.007 | 0.0006 | 0.0073 | 0.0733 | 0.0086 | 0.0008 |
Punctate Nuclear | 0.0216 | 0.0333 | 0.024 | 0.0499 | 0.0872 | 0.0414 | 0.0092 | 0.0166 | 0.0055 | 0.0068 | 0.008 | 0.0068 | 0.0047 | 0.0037 | 0.0035 | 0.0156 | 0.0127 | 0.0092 |
Vacuole | 0.0096 | 0.0021 | 0.003 | 0.0016 | 0.0016 | 0.0023 | 0.0074 | 0.0041 | 0.0022 | 0.0022 | 0.0043 | 0.0016 | 0.0022 | 0.0016 | 0.0024 | 0.002 | 0.0014 | 0.002 |
Vacuole Periphery | 0.0026 | 0.0003 | 0.0011 | 0.0006 | 0.0005 | 0.0012 | 0.002 | 0.0005 | 0.0006 | 0.0009 | 0.0005 | 0.0003 | 0.0003 | 0.0001 | 0.0009 | 0.0006 | 0.0005 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.6794 | 28.2914 | 24.9652 | 23.5533 | 21.071 | 21.372 | 33.6307 | 29.4819 | 29.1444 | 24.1016 |
Translational Efficiency | 2.1917 | 2.0781 | 1.5707 | 1.5465 | 1.7228 | 1.6556 | 1.6775 | 1.6352 | 1.305 | 1.5344 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1751 | 1549 | 319 | 1150 | 1799 | 1252 | 1970 | 1520 | 3550 | 2801 | 2289 | 2670 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 746.14 | 773.48 | 988.97 | 1050.25 | 711.42 | 756.26 | 949.85 | 1081.22 | 728.55 | 765.78 | 955.30 | 1067.88 |
Standard Deviation | 87.64 | 111.18 | 124.35 | 149.96 | 90.56 | 708.79 | 141.67 | 154.06 | 90.81 | 481.11 | 140.04 | 153.08 |
Intensity Change Log 2 | — | 0.051918 | 0.406480 | 0.493215 | — | 0.088181 | 0.416998 | 0.603887 | — | 0.069731 | 0.411624 | 0.548293 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000393 | 0.000296 | 0.001438 | 0.003007 | 0.000092 | 0.000415 | 0.000263 | 0.001459 | 0.000240 | 0.000349 | 0.000427 | 0.002126 |
Bud Neck | 0.008881 | 0.010047 | 0.005556 | 0.018741 | 0.004846 | 0.018595 | 0.009183 | 0.018369 | 0.006837 | 0.013868 | 0.008678 | 0.018529 |
Bud Site | 0.008589 | 0.005843 | 0.010168 | 0.090668 | 0.002668 | 0.009183 | 0.005034 | 0.053827 | 0.005589 | 0.007336 | 0.005750 | 0.069695 |
Cell Periphery | 0.000226 | 0.000140 | 0.000323 | 0.000250 | 0.000120 | 0.000212 | 0.000105 | 0.000197 | 0.000172 | 0.000172 | 0.000135 | 0.000220 |
Cytoplasm | 0.494357* | 0.468109* | 0.202189* | 0.110059* | 0.500258* | 0.418106* | 0.287084* | 0.189234* | 0.497347* | 0.445759* | 0.275253* | 0.155132* |
Cytoplasmic Foci | 0.093117 | 0.092804 | 0.008317 | 0.042404 | 0.110893 | 0.163754 | 0.001795 | 0.032226 | 0.102125 | 0.124518 | 0.002704 | 0.036610 |
Eisosomes | 0.000063 | 0.000060 | 0.000078 | 0.000034 | 0.000059 | 0.000113 | 0.000011 | 0.000029 | 0.000061 | 0.000084 | 0.000020 | 0.000031 |
Endoplasmic Reticulum | 0.001049 | 0.001988 | 0.013104 | 0.003606 | 0.000930 | 0.001233 | 0.002426 | 0.003594 | 0.000989 | 0.001651 | 0.003914 | 0.003600 |
Endosome | 0.003500 | 0.005941 | 0.004442 | 0.020590 | 0.004152 | 0.011417 | 0.000810 | 0.015625 | 0.003830 | 0.008389 | 0.001316 | 0.017763 |
Golgi | 0.000888 | 0.001402 | 0.000203 | 0.011237 | 0.000864 | 0.003225 | 0.000079 | 0.006843 | 0.000876 | 0.002217 | 0.000097 | 0.008736 |
Lipid Particles | 0.004204 | 0.003794 | 0.002828 | 0.001925 | 0.004471 | 0.010030 | 0.000289 | 0.001604 | 0.004339 | 0.006582 | 0.000643 | 0.001742 |
Mitochondria | 0.003889 | 0.002977 | 0.003734 | 0.010176 | 0.003895 | 0.010819 | 0.001056 | 0.006542 | 0.003892 | 0.006482 | 0.001429 | 0.008107 |
Mitotic Spindle | 0.002378 | 0.003514 | 0.003599 | 0.427734* | 0.002166 | 0.003972 | 0.021427 | 0.354404* | 0.002270 | 0.003719 | 0.018943 | 0.385988* |
None | 0.004571 | 0.007260 | 0.003462 | 0.005076 | 0.007201 | 0.006351 | 0.009167 | 0.014208 | 0.005904 | 0.006854 | 0.008372 | 0.010275 |
Nuclear Periphery | 0.001111 | 0.000870 | 0.002298 | 0.002298 | 0.000635 | 0.000923 | 0.001414 | 0.001557 | 0.000870 | 0.000894 | 0.001537 | 0.001876 |
Nuclear Periphery Foci | 0.000369 | 0.001208 | 0.005873 | 0.006301 | 0.000647 | 0.000734 | 0.000658 | 0.006626 | 0.000510 | 0.000996 | 0.001385 | 0.006486 |
Nucleolus | 0.001171 | 0.002999 | 0.000694 | 0.004668 | 0.001381 | 0.003092 | 0.000520 | 0.005203 | 0.001277 | 0.003041 | 0.000544 | 0.004973 |
Nucleus | 0.341099* | 0.346447* | 0.638546* | 0.158685* | 0.331087* | 0.281612* | 0.642971* | 0.232582* | 0.336025* | 0.317467* | 0.642354* | 0.200754* |
Peroxisomes | 0.001484 | 0.004217 | 0.000652 | 0.011019 | 0.002758 | 0.011566 | 0.000365 | 0.011003 | 0.002130 | 0.007502 | 0.000405 | 0.011010 |
Vacuole | 0.028124 | 0.039218 | 0.092221 | 0.054909 | 0.020402 | 0.043196 | 0.015244 | 0.039206 | 0.024211 | 0.040996 | 0.025972 | 0.045969 |
Vacuole Periphery | 0.000537 | 0.000865 | 0.000277 | 0.016612 | 0.000474 | 0.001453 | 0.000099 | 0.005660 | 0.000505 | 0.001128 | 0.000124 | 0.010377 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.34 | -4.92 | -3.85 | -6.05 | 0.91 | -3.11 | -9.71 | -7.89 | -4.28 | -5.06 | -0.94 | -3.30 | -6.64 | -7.63 | -5.59 |
Bud Neck | -0.96 | 2.12 | -12.15 | -10.51 | -13.31 | -6.99 | -7.04 | -15.00 | -4.64 | -8.21 | -6.15 | -5.33 | -18.98 | -10.65 | -11.63 |
Bud Site | 1.68 | -0.30 | -11.94 | -13.35 | -10.62 | -4.92 | -4.68 | -16.05 | -12.71 | -14.18 | -1.78 | -1.65 | -19.20 | -18.79 | -18.60 |
Cell Periphery | 2.87 | -2.93 | 1.15 | -3.98 | 4.95 | -7.13 | 0.10 | -5.88 | 1.90 | -5.64 | 0.13 | 1.24 | -0.77 | -1.57 | -3.33 |
Cytoplasm | 3.82 | 27.34 | 53.41 | 43.34 | 6.93 | 8.48 | 32.83 | 43.90 | 28.36 | 13.11 | 8.47 | 42.94 | 66.89 | 49.36 | 18.45 |
Cytoplasmic Foci | -0.12 | 24.67 | 18.19 | 16.86 | -5.68 | -8.26 | 30.06 | 21.89 | 25.52 | -11.37 | -5.62 | 39.97 | 28.39 | 29.64 | -13.51 |
Eisosomes | 0.13 | -3.87 | 2.29 | 3.10 | 6.05 | -10.36 | 16.80 | 2.66 | 12.13 | -15.28 | -6.58 | 10.90 | 3.31 | 11.12 | -10.97 |
Endoplasmic Reticulum | -6.65 | -12.53 | -11.84 | -8.31 | 8.98 | -2.02 | -10.15 | -12.95 | -12.46 | -3.80 | -6.28 | -15.49 | -17.66 | -14.18 | 1.24 |
Endosome | -4.24 | -2.54 | -5.12 | -1.89 | -3.41 | -6.39 | 7.46 | -3.87 | 3.28 | -8.66 | -7.54 | 7.71 | -6.36 | 1.22 | -10.78 |
Golgi | -1.12 | 1.84 | -2.85 | -2.36 | -4.34 | -3.39 | 3.60 | -2.26 | 1.30 | -3.92 | -3.42 | 3.56 | -3.57 | -0.59 | -5.91 |
Lipid Particles | 0.79 | 2.15 | 4.98 | 5.18 | 3.40 | -4.77 | 11.40 | 7.36 | 7.33 | -11.05 | -3.71 | 12.18 | 8.54 | 8.68 | -9.90 |
Mitochondria | 1.16 | 0.98 | 0.24 | -1.22 | -0.90 | -3.36 | 2.75 | 1.16 | 4.35 | -2.27 | -2.39 | 3.82 | 1.13 | 3.63 | -3.63 |
Mitotic Spindle | -1.06 | -1.24 | -31.61* | -31.49* | -30.39* | -1.31 | -9.72 | -32.33* | -32.12* | -27.26* | -1.67 | -9.83 | -45.09 | -44.86* | -38.22* |
None | -3.03 | 2.33 | -5.29 | -2.22 | -8.13 | 0.91 | -2.86 | -4.93 | -5.35 | -3.46 | -1.16 | -4.86 | -7.20 | -5.83 | -3.92 |
Nuclear Periphery | 0.88 | -6.77 | -10.88 | -16.27 | -4.87 | -2.43 | -19.44 | -20.72 | -14.71 | -7.86 | -0.43 | -12.22 | -19.48 | -22.29 | -10.45 |
Nuclear Periphery Foci | -5.58 | -6.01 | -9.53 | -8.78 | -3.81 | 0.35 | -2.65 | -8.48 | -8.65 | -7.14 | -2.63 | -4.73 | -12.30 | -11.95 | -9.15 |
Nucleolus | -3.06 | 2.85 | -9.99 | -8.46 | -10.28 | -3.18 | -2.64 | -11.32 | -9.28 | -9.44 | -4.27 | -2.93 | -15.04 | -12.45 | -12.70 |
Nucleus | -0.84 | -17.77 | 7.02 | 7.25 | 20.52 | 4.98 | -36.71 | 4.50 | -0.12 | 35.69 | 2.47 | -43.74 | 8.05 | 5.48 | 44.09 |
Peroxisomes | -6.31 | 5.24 | -5.73 | -1.50 | -7.13 | -6.41 | 4.14 | -2.50 | 4.52 | -6.62 | -7.98 | 5.35 | -5.34 | 2.83 | -9.48 |
Vacuole | -6.24 | -10.88 | -11.77 | -7.78 | 4.49 | -9.37 | -4.31 | -14.73 | -7.16 | -11.97 | -11.06 | -8.60 | -18.61 | -10.62 | -10.42 |
Vacuole Periphery | -1.44 | 2.61 | -2.71 | -2.36 | -2.98 | -3.99 | 6.41 | -1.93 | 0.38 | -2.90 | -3.79 | 6.88 | -3.29 | -1.97 | -4.10 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|