Standard name | |
---|---|
Human Ortholog | |
Description | Beta-adaptin; large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 186 | 222 | 161 | 86 | 130 | 147 | 169 | 158 | 130 | 142 | 128 | 182 | 67 | 104 | 123 | 92 | 112 | 68 | 121 | 192 | 237 | 56 | 106 | 109 |
Bud | 52 | 83 | 58 | 23 | 71 | 53 | 103 | 100 | 69 | 100 | 80 | 104 | 22 | 27 | 30 | 29 | 25 | 19 | 44 | 58 | 29 | 28 | 47 | 55 |
Bud Neck | 11 | 14 | 3 | 7 | 12 | 3 | 3 | 5 | 2 | 6 | 1 | 3 | 0 | 7 | 13 | 14 | 26 | 6 | 90 | 123 | 91 | 63 | 57 | 59 |
Bud Site | 83 | 106 | 77 | 49 | 81 | 105 | 63 | 57 | 40 | 51 | 36 | 55 | 14 | 12 | 22 | 69 | 40 | 25 | – | – | – | – | – | – |
Cell Periphery | 26 | 35 | 44 | 14 | 13 | 22 | 59 | 46 | 41 | 57 | 49 | 102 | 16 | 38 | 35 | 19 | 26 | 17 | 23 | 16 | 33 | 8 | 13 | 17 |
Cytoplasm | 15 | 37 | 39 | 19 | 27 | 28 | 26 | 52 | 31 | 39 | 34 | 36 | 15 | 28 | 41 | 24 | 26 | 22 | 1 | 7 | 2 | 1 | 2 | 3 |
Endoplasmic Reticulum | 12 | 16 | 10 | 8 | 30 | 20 | 48 | 39 | 33 | 23 | 33 | 47 | 6 | 8 | 11 | 5 | 9 | 7 | 1 | 5 | 6 | 4 | 5 | 4 |
Endosome | 0 | 3 | 9 | 2 | 5 | 5 | 10 | 15 | 1 | 4 | 2 | 2 | 0 | 0 | 1 | 2 | 0 | 0 | 2 | 4 | 3 | 2 | 3 | 2 |
Golgi | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 2 | 0 | 2 | 0 | 0 |
Mitochondria | 25 | 67 | 31 | 19 | 46 | 84 | 149 | 165 | 185 | 350 | 253 | 285 | 2 | 0 | 0 | 29 | 24 | 8 | 2 | 9 | 5 | 3 | 9 | 4 |
Nucleus | 10 | 7 | 8 | 6 | 6 | 10 | 14 | 18 | 3 | 9 | 5 | 8 | 0 | 1 | 7 | 23 | 6 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 2 | 2 | 1 | 3 | 0 | 9 | 6 | 6 | 5 | 2 | 5 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 1 | 0 |
Nucleolus | 1 | 1 | 4 | 5 | 4 | 8 | 21 | 33 | 15 | 31 | 22 | 14 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 12 | 8 | 80 | 44 | 77 | 99 | 124 | 100 | 56 | 58 | 41 | 95 | 2 | 0 | 3 | 19 | 9 | 13 | 1 | 1 | 4 | 0 | 1 | 1 |
SpindlePole | 13 | 3 | 14 | 6 | 5 | 2 | 7 | 16 | 2 | 7 | 3 | 3 | 3 | 12 | 28 | 29 | 20 | 14 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 3 | 4 | 1 | 0 | 7 | 1 | 5 | 16 | 5 | 13 | 1 | 9 | 1 | 0 | 1 | 2 | 1 | 0 | 3 | 5 | 1 | 1 | 1 | 1 |
Unique Cell Count | 256 | 356 | 334 | 178 | 333 | 354 | 502 | 528 | 359 | 568 | 411 | 548 | 92 | 167 | 219 | 213 | 185 | 137 | 296 | 433 | 421 | 175 | 254 | 264 |
Labelled Cell Count | 451 | 608 | 542 | 289 | 518 | 587 | 810 | 827 | 619 | 895 | 690 | 950 | 148 | 237 | 316 | 356 | 324 | 205 | 296 | 433 | 421 | 175 | 254 | 264 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.7 | 6.9 | 7.6 | 7.6 | 7.2 | 6.7 | 6.5 | 6.7 | 6.2 | 5.7 | 5.8 | 6.2 | 7.6 | 8.1 | 8.3 | 6.4 | 7.1 | 8.7 | 8.8 | 8.8 | 9.3 |
Std Deviation (1e-4) | 1.1 | 1.0 | 1.5 | 1.3 | 1.5 | 1.5 | 1.4 | 1.7 | 1.4 | 1.3 | 1.4 | 1.3 | 0.9 | 0.9 | 1.0 | 1.1 | 1.4 | 1.7 | 1.7 | 1.6 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.0 | -0.08 | -0.19 | -0.24 | -0.19 | -0.3 | -0.42 | -0.38 | -0.3 | 0.0 | 0.09 | 0.12 | -0.25 | -0.11 | 0.19 | 0.22 | 0.21 | 0.29 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | -2.4 | -1.8 | -4.2 | -5.4 | -3.2 | -7.1 | -4.8 | -4.4 | 4.2 | 3.0 | 1.8 | -1.1 | 2.7 | 0.3 |
Bud | -1.3 | 1.3 | -0.9 | 1.1 | 0.6 | 0.6 | 0.1 | 0.7 | 0.6 | 1.4 | -0.3 | -1.2 | -1.2 | -1.1 | -0.9 |
Bud Neck | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 | 3.7 | 6.2 | 0 |
Bud Site | 1.1 | 0.4 | 2.0 | -4.0 | -4.8 | -4.2 | -5.8 | -5.4 | -5.3 | -1.6 | -4.4 | -3.9 | 2.4 | -0.4 | -1.1 |
Cell Periphery | -1.8 | -4.3 | -3.1 | -0.6 | -2.1 | -0.7 | -1.4 | -0.5 | 2.1 | 1.0 | 2.7 | 0.9 | -1.5 | 0.3 | -0.2 |
Cytoplasm | -0.3 | -1.5 | -1.7 | -3.4 | -0.9 | -1.3 | -2.5 | -1.6 | -2.6 | 1.2 | 1.6 | 2.3 | -0.1 | 0.8 | 1.3 |
Endoplasmic Reticulum | 0.9 | 3.3 | 1.7 | 3.7 | 2.7 | 3.4 | 0.8 | 2.9 | 3.3 | 0 | 1.0 | 1.2 | -0.5 | 1.1 | 0 |
Endosome | 0 | -1.1 | -1.2 | -0.7 | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.5 | 1.8 | 5.1 | 7.0 | 7.5 | 12.0 | 15.4 | 14.6 | 12.8 | -2.3 | -4.1 | -4.6 | 1.6 | 1.3 | -1.2 |
Nucleus | 0 | -0.5 | 0.4 | 0.3 | 0.8 | -1.6 | -0.9 | -1.2 | -1.0 | 0 | 0 | 0.6 | 4.1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1.1 | 2.5 | 3.6 | 2.4 | 3.2 | 3.1 | 1.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0.2 | -0.3 | 1.2 | 0.2 | -1.8 | -2.8 | -5.5 | -5.1 | -2.4 | -4.7 | -6.9 | -7.3 | -4.5 | -5.5 | -3.6 |
SpindlePole | -0.5 | -2.1 | -3.2 | -2.5 | -0.9 | -3.2 | -2.8 | -3.1 | -3.8 | 0 | 1.4 | 3.7 | 4.0 | 2.9 | 2.5 |
Vacuole | 0 | 0 | 0 | 0 | 2.8 | 0 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.2869 | 4.574 | 4.1763 | 4.0019 | 4.2685 | 4.5183 | 4.493 | 5.1739 | 5.111 | 4.4966 | 4.9033 | 5.2792 | 5.37 | 5.499 | 5.7711 | 5.6355 | 5.4363 | 5.7619 |
Actin | 0.2158 | 0.1164 | 0.1297 | 0.0686 | 0.1822 | 0.2019 | 0.2612 | 0.103 | 0.131 | 0.0963 | 0.112 | 0.2174 | 0.1833 | 0.097 | 0.1444 | 0.1179 | 0.136 | 0.1793 |
Bud | 0.0052 | 0.0037 | 0.002 | 0.0008 | 0.0035 | 0.0034 | 0.0026 | 0.0025 | 0.0017 | 0.0011 | 0.0026 | 0.0029 | 0.0029 | 0.0046 | 0.0019 | 0.0065 | 0.0017 | 0.0033 |
Bud Neck | 0.1951 | 0.0497 | 0.6373 | 0.8526 | 0.7498 | 0.6886 | 0.1752 | 0.0406 | 0.5693 | 0.8453 | 0.6768 | 0.6907 | 0.2144 | 0.0633 | 0.5898 | 0.8334 | 0.7507 | 0.6912 |
Bud Periphery | 0.0201 | 0.0086 | 0.0072 | 0.0025 | 0.0263 | 0.0241 | 0.0068 | 0.0031 | 0.0057 | 0.0054 | 0.0232 | 0.0205 | 0.0081 | 0.0093 | 0.0066 | 0.0128 | 0.0158 | 0.0221 |
Bud Site | 0.3902 | 0.6803 | 0.1878 | 0.0371 | 0.0123 | 0.0106 | 0.3829 | 0.8038 | 0.2339 | 0.014 | 0.0182 | 0.0141 | 0.3976 | 0.7254 | 0.2357 | 0.0087 | 0.045 | 0.0119 |
Cell Periphery | 0.0562 | 0.0209 | 0.0089 | 0.0035 | 0.0184 | 0.0224 | 0.0534 | 0.0198 | 0.0151 | 0.0058 | 0.0457 | 0.0349 | 0.0968 | 0.0137 | 0.0127 | 0.0074 | 0.0364 | 0.0479 |
Cytoplasm | 0.0623 | 0.1003 | 0.0057 | 0.0016 | 0.0022 | 0.0314 | 0.0432 | 0.023 | 0.007 | 0.0016 | 0.0093 | 0.0147 | 0.0564 | 0.0649 | 0.0038 | 0.0066 | 0.005 | 0.03 |
Cytoplasmic Foci | 0.007 | 0.0032 | 0.0064 | 0.0006 | 0.0004 | 0.0021 | 0.0159 | 0.0006 | 0.004 | 0.0036 | 0.01 | 0.0006 | 0.0023 | 0.0063 | 0.0006 | 0.0005 | 0.0011 | 0.002 |
Eisosomes | 0.0054 | 0.0024 | 0.0014 | 0.0005 | 0.002 | 0.002 | 0.004 | 0.0014 | 0.0038 | 0.001 | 0.0016 | 0.0021 | 0.023 | 0.0009 | 0.0016 | 0.0016 | 0.0023 | 0.0033 |
Endoplasmic Reticulum | 0.0031 | 0.0022 | 0.0002 | 0.0035 | 0.0002 | 0.0015 | 0.005 | 0.0003 | 0.0006 | 0.0003 | 0.0022 | 0.0003 | 0.001 | 0.0011 | 0.0001 | 0.0013 | 0.0001 | 0.0015 |
Endosome | 0.0021 | 0.0006 | 0.0004 | 0.0032 | 0.0003 | 0.0008 | 0.0071 | 0.0001 | 0.0011 | 0.0021 | 0.0113 | 0.0001 | 0.0001 | 0.0068 | 0.0001 | 0.0002 | 0.0001 | 0.0016 |
Golgi | 0.0027 | 0.0018 | 0.0011 | 0.0005 | 0.001 | 0.0015 | 0.0066 | 0.0006 | 0.0026 | 0.0027 | 0.0148 | 0.0006 | 0.0011 | 0.0033 | 0.0005 | 0.0009 | 0.0005 | 0.0036 |
Lipid Particles | 0.0025 | 0.0011 | 0.0017 | 0.0002 | 0.0002 | 0.0009 | 0.0075 | 0.0002 | 0.001 | 0.0057 | 0.0201 | 0.0001 | 0.0007 | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0.0004 |
Mitochondria | 0.0004 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0003 | 0.0031 | 0 | 0.0015 | 0.0009 | 0.0101 | 0 | 0.0068 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 |
None | 0.021 | 0.0045 | 0.0054 | 0.0005 | 0.0001 | 0.0055 | 0.0039 | 0.0003 | 0.0157 | 0.0015 | 0.0005 | 0.0002 | 0.0019 | 0.001 | 0.0012 | 0.0002 | 0.0004 | 0.0003 |
Nuclear Periphery | 0.0005 | 0.0007 | 0.0003 | 0.0133 | 0.0001 | 0.0004 | 0.0071 | 0.0001 | 0.0001 | 0.0004 | 0.0084 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0005 | 0 | 0.0001 |
Nucleolus | 0.0014 | 0.0003 | 0.0004 | 0.0009 | 0.0002 | 0.0003 | 0.0009 | 0 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Nucleus | 0.0013 | 0.0007 | 0.0005 | 0.0072 | 0.0003 | 0.0004 | 0.001 | 0.0003 | 0.0002 | 0.0002 | 0.0008 | 0.0002 | 0.0005 | 0.0002 | 0.0002 | 0.0004 | 0.0004 | 0.0002 |
Peroxisomes | 0.001 | 0.001 | 0.0021 | 0.0002 | 0.0001 | 0.0005 | 0.0047 | 0.0002 | 0.0047 | 0.0105 | 0.0283 | 0.0001 | 0.0008 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0003 |
Punctate Nuclear | 0.0007 | 0.0004 | 0.001 | 0.0009 | 0.0001 | 0.0003 | 0.0058 | 0.0001 | 0.0003 | 0.0012 | 0.0011 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0037 | 0.0001 |
Vacuole | 0.0058 | 0.0011 | 0.0003 | 0.0011 | 0.0004 | 0.0011 | 0.0013 | 0.0003 | 0.0004 | 0.0002 | 0.0016 | 0.0003 | 0.0007 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0005 |
Vacuole Periphery | 0.0002 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0.0009 | 0 | 0 | 0.0001 | 0.0009 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 24.66 | 21.1065 | 22.6228 | 25.4545 | 14.966 | 28.3171 | 25.846 | 29.2872 | 28.0692 | 29.8473 |
Translational Efficiency | 0.6351 | 0.6949 | 0.6423 | 0.666 | 0.8566 | 0.6473 | 0.7124 | 0.6852 | 0.6469 | 0.6137 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1207 | 715 | 669 | 279 | 2389 | 1845 | 215 | 244 | 3596 | 2560 | 884 | 523 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1053.95 | 934.06 | 1233.45 | 1193.07 | 886.61 | 885.30 | 1190.91 | 1133.76 | 942.78 | 898.92 | 1223.10 | 1165.40 |
Standard Deviation | 137.31 | 157.59 | 162.02 | 169.39 | 113.23 | 138.77 | 142.59 | 152.83 | 145.22 | 145.92 | 158.57 | 164.56 |
Intensity Change Log 2 | — | -0.174219 | 0.226893 | 0.178872 | — | -0.002133 | 0.425693 | 0.354744 | — | -0.093042 | 0.321131 | 0.261893 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.092973 | 0.186798* | 0.200853* | 0.241085* | 0.140665* | 0.142419* | 0.101022* | 0.260334* | 0.124657* | 0.154814* | 0.176573* | 0.250066* |
Bud Neck | 0.219102* | 0.189709* | 0.200856* | 0.248335* | 0.274597* | 0.255739* | 0.277326* | 0.269756* | 0.255970* | 0.237297* | 0.219454* | 0.258328* |
Bud Site | 0.546620* | 0.360363* | 0.455759* | 0.381398* | 0.256241* | 0.340185* | 0.499790* | 0.256617* | 0.353707* | 0.345821* | 0.466468* | 0.323183* |
Cell Periphery | 0.054174 | 0.095711 | 0.026116 | 0.059244 | 0.211328* | 0.128247* | 0.061759 | 0.050147 | 0.158579* | 0.119160* | 0.034785 | 0.055000 |
Cytoplasm | 0.032704 | 0.033976 | 0.028121 | 0.006245 | 0.032422 | 0.037501 | 0.007485 | 0.011026 | 0.032516 | 0.036516 | 0.023102 | 0.008475 |
Cytoplasmic Foci | 0.009173 | 0.023524 | 0.019956 | 0.005166 | 0.007292 | 0.013008 | 0.002942 | 0.024190 | 0.007923 | 0.015945 | 0.015818 | 0.014042 |
Eisosomes | 0.004147 | 0.009362 | 0.005176 | 0.016553 | 0.026163 | 0.012406 | 0.006514 | 0.011247 | 0.018774 | 0.011556 | 0.005502 | 0.014078 |
Endoplasmic Reticulum | 0.003258 | 0.007361 | 0.004058 | 0.005182 | 0.002722 | 0.002712 | 0.004966 | 0.003948 | 0.002902 | 0.004011 | 0.004279 | 0.004606 |
Endosome | 0.006491 | 0.022965 | 0.011820 | 0.009575 | 0.004248 | 0.011781 | 0.003442 | 0.036316 | 0.005001 | 0.014905 | 0.009782 | 0.022051 |
Golgi | 0.003299 | 0.022158 | 0.003530 | 0.002118 | 0.004957 | 0.010934 | 0.001788 | 0.013514 | 0.004400 | 0.014069 | 0.003106 | 0.007435 |
Lipid Particles | 0.003381 | 0.012637 | 0.007086 | 0.005112 | 0.002987 | 0.004647 | 0.003161 | 0.028898 | 0.003119 | 0.006879 | 0.006131 | 0.016209 |
Mitochondria | 0.001415 | 0.007088 | 0.000708 | 0.000778 | 0.014798 | 0.016306 | 0.000695 | 0.001763 | 0.010306 | 0.013732 | 0.000705 | 0.001238 |
Mitotic Spindle | 0.001101 | 0.002211 | 0.001561 | 0.000356 | 0.000231 | 0.000466 | 0.000461 | 0.000743 | 0.000523 | 0.000954 | 0.001293 | 0.000536 |
None | 0.000030 | 0.000150 | 0.000020 | 0.000016 | 0.000211 | 0.000827 | 0.000012 | 0.000018 | 0.000150 | 0.000638 | 0.000018 | 0.000017 |
Nuclear Periphery | 0.000034 | 0.000025 | 0.000036 | 0.000038 | 0.000014 | 0.000017 | 0.000033 | 0.000033 | 0.000021 | 0.000019 | 0.000035 | 0.000036 |
Nuclear Periphery Foci | 0.000418 | 0.000398 | 0.001325 | 0.000458 | 0.000082 | 0.000165 | 0.000884 | 0.001512 | 0.000195 | 0.000230 | 0.001217 | 0.000950 |
Nucleolus | 0.000016 | 0.000049 | 0.000023 | 0.000016 | 0.000027 | 0.000050 | 0.000014 | 0.000025 | 0.000023 | 0.000050 | 0.000021 | 0.000020 |
Nucleus | 0.001141 | 0.000926 | 0.000997 | 0.001123 | 0.001281 | 0.001385 | 0.005686 | 0.000278 | 0.001234 | 0.001257 | 0.002138 | 0.000728 |
Peroxisomes | 0.004202 | 0.019403 | 0.009976 | 0.006765 | 0.017952 | 0.017056 | 0.004301 | 0.021453 | 0.013337 | 0.017711 | 0.008595 | 0.013618 |
Vacuole | 0.015529 | 0.004402 | 0.021837 | 0.010345 | 0.001459 | 0.003157 | 0.017624 | 0.007998 | 0.006182 | 0.003505 | 0.020813 | 0.009250 |
Vacuole Periphery | 0.000791 | 0.000782 | 0.000186 | 0.000092 | 0.000323 | 0.000992 | 0.000093 | 0.000183 | 0.000480 | 0.000933 | 0.000163 | 0.000134 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -8.55 | -8.24 | -8.01 | -2.60 | -1.90 | -0.24 | 2.95 | -6.09 | -5.93 | -6.94 | -5.02 | -4.97 | -9.31 | -6.83 | -4.64 |
Bud Neck | 2.34 | 1.65 | -1.39 | -2.75 | -2.33 | 2.07 | 0.04 | 0.28 | -0.66 | 0.18 | 2.50 | 3.94 | -0.02 | -1.33 | -2.54 |
Bud Site | 12.31 | 6.04 | 7.77 | -0.75 | 3.31 | -9.58 | -10.78 | -0.02 | 4.29 | 8.46 | 0.93 | -9.35 | 2.20 | 1.67 | 8.26 |
Cell Periphery | -5.66 | 6.56 | -0.51 | 3.61 | -3.89 | 11.59 | 14.89 | 16.85 | 8.51 | 0.95 | 7.11 | 25.43 | 15.06 | 9.46 | -3.19 |
Cytoplasm | -0.02 | 0.79 | 5.37 | 5.00 | 4.06 | -1.85 | 8.78 | 4.99 | 5.89 | -0.84 | -1.55 | 2.51 | 7.48 | 8.13 | 3.74 |
Cytoplasmic Foci | -4.89 | -3.74 | 0.80 | 4.08 | 3.34 | -4.28 | 3.64 | -3.12 | -2.05 | -3.83 | -6.26 | -3.88 | -2.17 | 0.33 | 0.45 |
Eisosomes | -4.71 | -1.00 | -3.26 | -1.81 | -2.93 | 7.74 | 9.94 | 5.34 | 0.47 | -1.76 | 5.70 | 10.60 | 1.92 | -1.01 | -3.56 |
Endoplasmic Reticulum | -2.82 | -0.79 | -1.34 | 0.82 | -0.70 | 0.02 | -1.79 | -1.24 | -1.30 | 0.84 | -1.83 | -1.67 | -1.86 | -0.78 | -0.27 |
Endosome | -5.57 | -2.83 | -1.02 | 3.31 | 0.70 | -6.48 | 0.57 | -5.49 | -4.14 | -5.53 | -8.36 | -3.58 | -5.32 | -2.13 | -3.54 |
Golgi | -7.25 | -0.29 | 1.77 | 7.75 | 2.11 | -4.96 | 3.62 | -3.14 | -0.89 | -4.28 | -8.57 | 2.24 | -2.23 | 4.02 | -3.21 |
Lipid Particles | -3.63 | -2.43 | -1.21 | 2.29 | 0.78 | -1.81 | -0.23 | -3.86 | -3.60 | -3.68 | -3.87 | -2.55 | -4.03 | -2.81 | -2.82 |
Mitochondria | -5.71 | 1.52 | 1.40 | 6.96 | -0.28 | -0.82 | 10.83 | 10.25 | 10.34 | -1.84 | -2.61 | 11.34 | 10.66 | 12.12 | -2.12 |
Mitotic Spindle | -0.82 | -0.85 | 1.55 | 1.41 | 2.60 | -2.87 | -1.95 | -1.88 | -0.98 | -0.96 | -1.10 | -2.16 | -0.14 | 1.03 | 2.11 |
None | -2.74 | 0.89 | 1.35 | 3.08 | 1.09 | -1.87 | 6.16 | 5.99 | 2.46 | -1.81 | -2.05 | 6.01 | 6.11 | 2.61 | 0.68 |
Nuclear Periphery | 0.76 | -0.48 | -0.87 | -1.77 | -0.64 | -1.13 | -1.87 | -4.10 | -3.13 | 0.27 | 0.39 | -2.92 | -2.57 | -3.03 | -0.24 |
Nuclear Periphery Foci | 0.35 | -2.53 | -0.94 | -1.02 | 1.07 | -2.78 | -2.96 | -2.44 | -2.30 | -0.72 | -0.80 | -3.58 | -2.62 | -2.54 | 0.52 |
Nucleolus | -2.57 | -2.16 | -0.46 | 1.88 | 0.50 | -2.15 | 3.19 | 0.33 | 2.03 | -1.36 | -3.11 | 0.36 | 0.19 | 2.74 | -0.00 |
Nucleus | 0.86 | 0.01 | -0.63 | -0.88 | -0.64 | -0.31 | -1.31 | 6.27 | 2.96 | 1.55 | -0.06 | -1.26 | 0.18 | 0.19 | 1.19 |
Peroxisomes | -6.38 | -3.10 | -1.03 | 3.74 | 1.03 | 0.46 | 6.06 | -0.92 | -1.15 | -4.22 | -2.89 | 2.87 | -0.15 | 1.62 | -2.00 |
Vacuole | 5.67 | -2.22 | 0.27 | -2.30 | 1.77 | -4.05 | -2.99 | -3.38 | -2.49 | 2.07 | 3.93 | -5.74 | -1.92 | -3.13 | 3.22 |
Vacuole Periphery | 0.02 | 1.36 | 1.59 | 4.09 | 1.59 | -2.71 | 3.53 | 2.06 | 3.31 | -1.56 | -1.93 | 2.02 | 2.24 | 4.39 | 0.69 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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