Standard name
Human Ortholog
Description Ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.05 0 0.06 – 0 0.06 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.99 0.98 0.98 0.98 0.95 0.94 – 0.89 0.87 0.73 0.82 1.0 0.99 0.99 0.92 0.97 0.95 0.86 0.9 0.6 0.88 0.86 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0.1 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.05 – 0.12 0.12 0.35 0.2 0 0 0 0.07 0 0 0.05 0 0.23 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 4 3 4 1 4 6
Bud 2 0 0 1 0 0 3 – 1 4 12 3 0 0 0 1 0 0 2 1 3 0 8 7
Bud Neck 1 1 13 10 17 9 25 – 12 16 10 18 1 1 0 1 3 0 2 0 1 1 1 1
Bud Site 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 3 4 2 1 2 1 – 0 1 1 3 2 0 1 2 4 6 1 2 4 1 1 1
Cytoplasm 404 353 298 290 353 358 402 – 252 240 208 264 274 395 331 135 232 189 353 319 173 216 322 334
Endoplasmic Reticulum 2 1 0 2 0 1 2 – 1 0 1 5 0 1 1 8 24 17 0 0 3 0 1 1
Endosome 0 0 0 0 0 0 1 – 1 0 0 0 0 0 0 2 1 6 4 1 3 1 4 1
Golgi 1 0 1 0 1 0 0 – 0 0 0 0 0 1 0 0 2 0 0 0 2 0 0 1
Mitochondria 12 1 0 2 1 9 23 – 34 33 99 66 0 1 0 10 1 2 20 11 65 5 9 10
Nucleus 0 1 2 0 1 4 3 – 1 2 1 1 1 0 2 4 1 0 0 0 0 1 1 0
Nuclear Periphery 0 0 0 0 0 1 1 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 1 0 1 0 0 0 0 0 1 1 0 0 0 0 0 0
Peroxisomes 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 2 3 3 3 7 – 7 7 11 19 0 0 2 0 0 1 5 2 5 2 7 7
Vac/Vac Membrane 0 1 3 2 1 5 0 – 1 0 2 1 0 0 0 4 2 4 6 2 5 0 3 2
Unique Cell Count 416 357 303 297 359 376 427 283 277 284 322 274 398 334 147 240 200 413 354 288 246 375 390
Labelled Cell Count 423 361 323 313 378 393 468 311 303 347 380 278 399 337 167 271 227 413 354 288 246 375 390


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.0 4.8 4.5 4.7 4.1 4.0 – 3.6 3.5 3.1 3.3 4.8 5.0 5.2 5.2 5.3 5.3 4.6 4.7 4.8
Std Deviation (1e-4) 0.4 0.7 1.3 1.6 1.7 1.9 1.5 – 1.6 1.2 1.2 0.9 1.0 1.2 5.0 1.5 1.6 1.9 1.4 1.3 1.5
Intensity Change (Log2) – – – -0.07 -0.01 -0.22 -0.24 – -0.41 -0.43 -0.61 -0.54 0.01 0.07 0.12 0.13 0.14 0.16 -0.06 -0.02 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.4479 -1.4114 -1.5123 -1.5851 -1.3124 -1.364 -0.6805 -0.5376 -0.4421 -0.7645 -1.0737 -0.5048 -1.5284 -1.1673 -1.2435 -0.8502 -1.4146 -1.1255
Actin 0.0183 0.0013 0.004 0.0205 0.0141 0.0098 0.0351 0.0017 0.0135 0.0019 0.0002 0.0018 0.0019 0.0004 0.0005 0.013 0.0005 0.001
Bud 0.0007 0.0013 0.0016 0.0002 0.0019 0.0009 0.0009 0.0017 0.0017 0.001 0.0046 0.0023 0.0002 0.0004 0.0003 0.0001 0.0005 0.0004
Bud Neck 0.0028 0.0003 0.0005 0.0004 0.0002 0.0012 0.0026 0.0003 0.0005 0.0003 0.0034 0.0011 0.0001 0.0009 0.0001 0.0001 0.0003 0.0008
Bud Periphery 0.0022 0.0044 0.0034 0.0003 0.0056 0.0007 0.0018 0.0018 0.0058 0.0026 0.0063 0.0023 0.0002 0.0003 0.0008 0.0002 0.0009 0.0006
Bud Site 0.005 0.0137 0.016 0.0038 0.003 0.0002 0.0095 0.0227 0.0051 0.0045 0.0073 0.0021 0.0018 0.0041 0.0021 0.0004 0.0005 0.0012
Cell Periphery 0.0006 0.0004 0.0003 0.0001 0.0001 0.0001 0.0003 0.0002 0.0004 0.0003 0.0003 0.0001 0.0001 0.0001 0.0001 0 0 0
Cytoplasm 0.018 0.0224 0.015 0.0158 0.0076 0.0293 0.0075 0.0093 0.0078 0.0085 0.0169 0.0115 0.0229 0.0246 0.0239 0.0342 0.0671 0.0413
Cytoplasmic Foci 0.0221 0.0071 0.0126 0.0211 0.0394 0.0126 0.0172 0.0027 0.0065 0.005 0.0069 0.0103 0.0077 0.0096 0.0034 0.009 0.0103 0.0034
Eisosomes 0.0007 0.0005 0.0004 0.0002 0.0002 0.0002 0.0006 0.0004 0.0006 0.0003 0.0001 0.0001 0.0002 0.0002 0.0002 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0073 0.0011 0.0004 0.0002 0.0001 0.0006 0.0013 0.0001 0.0005 0.0006 0.0003 0.0005 0.0003 0.0002 0.0002 0.0004 0.0002 0.0003
Endosome 0.0255 0.0009 0.0078 0.0033 0.0007 0.0078 0.0115 0 0.0021 0.0015 0.0005 0.0083 0.0016 0.0004 0.0004 0.0028 0.0004 0.0004
Golgi 0.0105 0.0002 0.0041 0.0044 0.0014 0.0017 0.0036 0.0001 0.0021 0.0004 0.0001 0.0015 0.0004 0 0.0001 0.0032 0.0001 0.0001
Lipid Particles 0.0175 0.0008 0.0081 0.0042 0.0013 0.0027 0.0118 0.0002 0.0039 0.0007 0.0008 0.0031 0.0024 0.0001 0.0002 0.0079 0.0005 0.0001
Mitochondria 0.0091 0.0006 0.0219 0.002 0.0048 0.0007 0.0028 0.0011 0.0078 0.0019 0.0004 0.0046 0.0003 0.0002 0.0006 0.0022 0.0007 0.0003
None 0.8351 0.9361 0.8956 0.8891 0.8787 0.9141 0.8688 0.9527 0.9188 0.9608 0.9187 0.9305 0.9542 0.9563 0.9627 0.9087 0.9 0.946
Nuclear Periphery 0.003 0.0004 0.0003 0.0004 0.0001 0.0025 0.0029 0.0001 0.0009 0.0007 0.0004 0.0054 0.0003 0.0001 0.0003 0.0003 0.0003 0.0004
Nucleolus 0.0007 0.0009 0.0002 0.0002 0.0002 0.0004 0.0014 0.0003 0.001 0.001 0.0063 0.0004 0.0002 0.0002 0.0003 0.0001 0.0023 0.0001
Nucleus 0.0011 0.003 0.0005 0.0004 0.0001 0.0016 0.001 0.0004 0.0037 0.0013 0.0057 0.0024 0.0004 0.0004 0.0009 0.0003 0.0014 0.0006
Peroxisomes 0.0135 0.0007 0.0055 0.0272 0.039 0.0046 0.0088 0.0033 0.0116 0.0005 0.0003 0.0024 0.0025 0.0001 0.0005 0.0154 0.0013 0.0002
Punctate Nuclear 0.0017 0.0034 0.0011 0.0059 0.0013 0.0069 0.0079 0.0009 0.0046 0.0047 0.0198 0.0084 0.002 0.001 0.0021 0.0013 0.0124 0.0023
Vacuole 0.0034 0.0005 0.0004 0.0001 0.0001 0.001 0.002 0.0001 0.0008 0.0012 0.0006 0.0004 0.0002 0.0002 0.0002 0.0003 0.0002 0.0002
Vacuole Periphery 0.0013 0.0001 0.0002 0.0001 0 0.0005 0.0007 0 0.0003 0.0004 0.0001 0.0005 0 0 0 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 38.3536 22.8572 29.3556 38.2123 29.645 28.0121 29.7775 36.146 37.6576 36.3373
Translational Efficiency 0.4167 0.6247 0.5913 0.4923 0.4012 0.5763 0.6337 0.4534 0.4111 0.399

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1930 1213 79 1443 1424 2303 108 2565 3354 3516 187 4008

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 617.20 666.71 881.37 762.72 619.95 651.36 836.96 733.18 618.37 656.66 855.72 743.82
Standard Deviation 61.84 108.46 365.13 133.62 71.09 82.69 68.72 99.33 65.94 92.69 243.99 113.77
Intensity Change Log 2 — 0.111321 0.514010 0.305415 — 0.071303 0.433007 0.242016 — 0.091407 0.473987 0.273993

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000032 0.000817 0.000298 0.001076 0.000163 0.000355 0.000280 0.000193 0.000087 0.000514 0.000288 0.000511
Bud Neck 0.007525 0.028880 0.004267 0.013940 0.016905 0.061656 0.001652 0.022094 0.011507 0.050348 0.002757 0.019159
Bud Site 0.003373 0.015600 0.015212 0.051243 0.003272 0.025362 0.001405 0.041172 0.003330 0.021994 0.007238 0.044798
Cell Periphery 0.000162 0.000166 0.000233 0.000343 0.000116 0.000169 0.000180 0.000200 0.000142 0.000168 0.000202 0.000252
Cytoplasm 0.583849* 0.423733* 0.103656* 0.419841* 0.461057* 0.413427* 0.227238* 0.546936* 0.531716* 0.416983* 0.175029* 0.501178*
Cytoplasmic Foci 0.108040 0.243689* 0.026432 0.019106 0.235663* 0.234031* 0.017556 0.009164 0.162225 0.237363* 0.021306 0.012743
Eisosomes 0.000134 0.000156 0.000126 0.000054 0.000153 0.000158 0.000114 0.000030 0.000142 0.000157 0.000119 0.000038
Endoplasmic Reticulum 0.000223 0.000648 0.004311 0.001677 0.000612 0.000447 0.004626 0.000632 0.000388 0.000516 0.004493 0.001009
Endosome 0.002609 0.025853 0.003351 0.006158 0.009118 0.020546 0.003500 0.002757 0.005373 0.022377 0.003437 0.003982
Golgi 0.000603 0.003973 0.000081 0.005463 0.002204 0.005769 0.000082 0.000343 0.001283 0.005149 0.000082 0.002187
Lipid Particles 0.003568 0.010255 0.005366 0.002581 0.009122 0.006144 0.003211 0.000413 0.005926 0.007563 0.004121 0.001194
Mitochondria 0.001394 0.007626 0.000421 0.001974 0.003147 0.016849 0.000261 0.003606 0.002139 0.013667 0.000329 0.003019
Mitotic Spindle 0.001468 0.003664 0.000558 0.059305 0.002828 0.008565 0.000664 0.038082 0.002046 0.006874 0.000619 0.045723
None 0.008817 0.006224 0.003180 0.010242 0.003590 0.002903 0.007391 0.008613 0.006597 0.004049 0.005612 0.009199
Nuclear Periphery 0.000219 0.000649 0.001715 0.001601 0.000499 0.000314 0.001732 0.000952 0.000338 0.000430 0.001725 0.001186
Nuclear Periphery Foci 0.000362 0.000634 0.000910 0.001156 0.000353 0.000191 0.001445 0.000253 0.000358 0.000344 0.001219 0.000578
Nucleolus 0.001047 0.005222 0.043172 0.001125 0.001884 0.001646 0.001057 0.000950 0.001402 0.002879 0.018849 0.001013
Nucleus 0.259576* 0.137290* 0.730664* 0.325257* 0.204298* 0.113902 0.590195* 0.272599* 0.236107* 0.121971 0.649538* 0.291558*
Peroxisomes 0.001076 0.013332 0.004238 0.001631 0.007993 0.017430 0.000404 0.000830 0.004013 0.016016 0.002024 0.001118
Vacuole 0.015660 0.069646 0.051587 0.073108 0.036351 0.067971 0.136870 0.048866 0.024445 0.068549 0.100841 0.057593
Vacuole Periphery 0.000263 0.001945 0.000225 0.003119 0.000672 0.002164 0.000136 0.001314 0.000437 0.002088 0.000173 0.001964

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.90 -5.52 -3.15 0.12 -1.44 -2.96 -2.14 -0.44 3.86 2.31 -2.94 -5.47 -3.53 0.74 -0.25
Bud Neck -8.73 1.39 -2.81 7.16 -2.91 -14.85 9.31 -0.19 15.50 -11.99 -18.70 6.45 -3.02 17.00 -9.28
Bud Site -6.00 -0.85 -9.34 -4.82 -1.93 -12.41 1.73 -11.84 -0.76 -12.33 -13.37 -0.72 -14.84 -3.43 -4.93
Cell Periphery -0.08 -2.35 -2.96 -2.55 0.74 -4.12 -2.25 -2.61 1.65 0.67 -2.28 -2.76 -2.87 -0.54 1.21
Cytoplasm 17.05 31.97 26.93 6.67 -15.41 5.19 11.02 -1.42 -7.22 -11.72 18.19 24.46 16.63 -1.79 -17.44
Cytoplasmic Foci -19.48 13.50 29.94 36.45 2.64 0.29 34.15 39.18 48.91 2.16 -15.26 33.55 47.09 60.96 3.50
Eisosomes -1.65 -1.07 7.84 10.07 5.33 -0.56 2.52 18.72 18.17 7.41 -1.87 0.64 16.65 20.53 9.05
Endoplasmic Reticulum -7.74 -5.88 -14.24 -11.78 3.36 1.40 -6.19 -3.69 -8.03 5.67 -2.08 -8.65 -14.70 -14.07 6.57
Endosome -13.74 -2.32 -1.97 12.91 0.87 -9.11 6.84 10.18 17.48 2.95 -17.80 2.98 7.26 21.61 2.91
Golgi -6.28 2.70 -2.08 1.55 -2.75 -5.06 4.31 4.05 11.02 -4.02 -8.73 5.06 0.73 8.59 -3.01
Lipid Particles -7.17 -2.67 2.68 8.43 4.14 3.61 7.61 11.41 14.74 6.39 -2.91 2.92 11.53 15.27 6.64
Mitochondria -7.28 2.47 -0.44 7.74 -7.16 -10.16 6.78 1.05 10.73 -6.32 -12.45 6.29 -0.19 12.54 -7.77
Mitotic Spindle -1.93 1.47 -9.64 -8.80 -9.99 -3.51 1.04 -10.15 -7.60 -11.18 -4.63 1.35 -14.40 -11.40 -15.07
None 2.36 4.19 1.56 -0.64 -2.81 1.16 -1.98 -5.67 -7.02 -1.02 4.22 1.75 -1.16 -5.64 -2.55
Nuclear Periphery -3.37 -7.57 -20.20 -11.82 -1.32 2.86 -9.96 -15.12 -21.57 3.26 -1.70 -12.79 -27.16 -22.46 1.57
Nuclear Periphery Foci -1.12 -3.07 -2.90 -3.24 0.93 2.04 -4.14 1.03 -1.19 4.46 0.18 -4.86 -1.49 -3.38 4.52
Nucleolus -5.48 -1.78 1.42 5.84 1.80 0.22 2.46 2.42 3.27 -0.41 -4.18 -1.69 2.84 6.64 1.78
Nucleus 18.20 -14.16 -14.10 -26.93 10.00 15.57 -12.14 -18.60 -36.30 7.17 26.80 -17.34 -21.65 -45.18 11.63
Peroxisomes -10.20 -1.31 0.96 10.28 1.47 -7.21 9.05 8.68 16.50 -0.97 -13.87 3.12 8.56 19.42 1.33
Vacuole -18.60 -4.49 -23.45 -11.98 -2.68 -11.14 -7.62 -15.20 -5.61 5.28 -22.63 -8.78 -29.07 -12.19 3.26
Vacuole Periphery -10.03 0.29 -3.22 1.12 -3.28 -6.61 6.40 -0.33 3.97 -2.28 -10.69 5.54 -2.57 3.93 -3.76
Standard name
Human Ortholog
Description Ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (20%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ubp13

Ubp13


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ubp13-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available