Standard name
Human Ortholog
Description Kinesin-related motor protein; required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning; functionally redundant with Cin8p for chromosomal but not plasmid functions
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.06 0.09 0.05 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0.05 0.05 0.05 0.1 0.08 0.09 0.06 0.08 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.21 0.26 0.31 0.3 0.15 0.16 0.14 0.19 0.14 0.13 0.09 0.11 0.23 0.34 0.39 0.16 0.14 0.09 0 0.1 0.08 0.12 0.11 0.12
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.35 0.17 0 0 0.05 0 0 0 0 0 0 0 0.19 0.17 0.12 0.18 0.28 0.15 0.13 0.09 0.08 0.11 0.14 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.15 0.23 0.43 0.25 0.54 0.58 0.47 0.59 0.61 0.68 0.66 0.06 0 0.09 0.07 0.08 0.12 0 0 0.07 0 0 0.07
Nucleus 0 0.05 0 0.1 0.12 0.14 0.12 0.13 0.11 0.16 0.1 0.11 0 0 0 0.12 0.06 0.13 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.18 0.11 0.05 0.12 0.16 0.15 0.18 0.15 0.12 0.14 0.12 0.1 0.19 0.21 0.3 0.33 0.36 0.37 0 0.06 0 0.06 0.06 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.5 0.38 0.46 0.34 0.53 0.3 0.26 0.39 0.31 0.37 0.32 0.34 0.5 0.46 0.37 0.48 0.55 0.53 0.58 0.52 0.58 0.48 0.47 0.49
Vac/Vac Membrane 0.05 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0.06 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 2
Bud 0 0 4 0 4 5 6 21 24 12 17 22 1 0 0 0 0 0 3 6 4 6 4 7
Bud Neck 0 3 16 6 12 5 7 8 11 5 6 12 2 2 1 1 0 2 1 2 0 3 0 3
Bud Site 0 3 9 6 15 8 12 37 23 23 14 25 1 0 3 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 24 86 73 62 47 24 34 67 38 31 22 37 32 63 73 23 16 11 6 39 24 19 22 26
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 4 5 0 0 1 0 2 0
Endosome 39 54 3 1 16 2 2 7 3 0 2 1 27 31 22 27 33 19 18 36 22 18 27 19
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3 0 2 1 1 2 1 0
Mitochondria 2 49 56 89 77 82 138 169 161 150 158 219 8 7 16 11 9 15 5 17 21 5 7 17
Nucleus 4 17 3 20 36 21 28 46 31 38 24 37 2 4 2 17 7 16 3 16 1 2 3 2
Nuclear Periphery 0 3 0 1 0 4 0 0 0 0 2 2 0 0 0 0 0 0 0 1 0 1 0 1
Nucleolus 20 37 11 24 50 23 42 53 33 35 29 34 26 39 56 48 42 46 4 23 11 9 11 6
Peroxisomes 4 4 0 0 2 0 0 4 7 3 1 0 6 8 3 2 3 2 0 0 1 0 0 1
SpindlePole 57 123 111 71 165 46 62 138 83 90 75 114 70 85 69 70 64 66 79 206 166 79 94 113
Vac/Vac Membrane 6 28 0 1 4 2 2 1 1 1 0 5 5 3 7 13 6 7 0 14 5 8 8 9
Unique Cell Count 113 325 239 207 309 153 236 358 271 245 234 331 140 183 187 147 116 125 138 396 288 167 200 229
Labelled Cell Count 156 407 286 281 428 222 333 551 415 388 350 508 180 242 252 220 187 190 138 396 288 167 200 229


Microtubule

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 3.7 3.3 3.3 3.6 3.3 4.0 3.2 4.5 2.7 2.8 2.7 4.7 4.6 4.1 6.5 7.4 7.3 4.6 4.6 4.9
Std Deviation (1e-4) 0.8 0.7 0.6 1.1 1.2 1.3 9.9 1.1 11.3 1.0 0.9 3.9 2.0 1.1 1.3 1.6 2.3 2.4 1.9 1.7 1.2
Intensity Change (Log2) – – – 0.01 0.12 -0.01 0.29 -0.06 0.45 -0.29 -0.27 -0.28 0.5 0.46 0.32 0.96 1.16 1.13 0.48 0.48 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7863 0.3556 0.6647 0.3434 0.8822 0.3311 -2.1465 -0.8623 -1.0161 -1.2733 -1.433 -1.1859 1.9261 3.6417 3.1692 2.6417 3.0932 2.6394
Actin 0.1096 0.0002 0.017 0.0084 0.0107 0.0019 0.0027 0.0001 0 0.0002 0.0005 0.0034 0.0006 0.0001 0.0069 0.0018 0.0006 0.0017
Bud 0.0037 0.0014 0.0003 0.0002 0.0009 0.0002 0.0002 0.0002 0 0 0 0.0001 0.0001 0 0.0001 0.0002 0 0.0001
Bud Neck 0.0193 0.0062 0.001 0.0185 0.0199 0.0303 0.0017 0.0016 0.0008 0.002 0.0065 0.0075 0.001 0.0008 0.0011 0.0013 0.0013 0.0068
Bud Periphery 0.0043 0.0017 0.0005 0.0002 0.0015 0.0004 0.0001 0.0001 0 0 0 0.0002 0.0001 0 0.0002 0.0012 0.0001 0.0002
Bud Site 0.0238 0.0123 0.0092 0.0017 0.0067 0.0008 0.0008 0.003 0.0003 0.0001 0.0002 0.0003 0.0008 0.0018 0.0007 0.0011 0.0003 0.0007
Cell Periphery 0.0006 0.0002 0.0002 0 0.0004 0.0002 0 0 0 0 0 0.0001 0.0001 0 0 0.0002 0 0.0007
Cytoplasm 0.0263 0.0388 0.0007 0.0003 0.0079 0.0286 0.0149 0.0081 0.0004 0.001 0.0067 0.0037 0.0321 0.0156 0.0032 0.0097 0.0461 0.014
Cytoplasmic Foci 0.0746 0.0507 0.0288 0.0511 0.0288 0.0485 0.0415 0.0062 0.0054 0.0033 0.0275 0.0213 0.0567 0.0093 0.0562 0.028 0.0073 0.0412
Eisosomes 0.0013 0 0.0002 0.0003 0.0002 0.0001 0 0 0 0 0 0.0001 0.0001 0.0001 0.0001 0.0006 0.0001 0.0005
Endoplasmic Reticulum 0.0074 0.0013 0.0012 0 0.0032 0.0012 0.0009 0.0011 0 0.0001 0.0002 0.0006 0.001 0.0009 0.0004 0.0003 0.0011 0.0008
Endosome 0.0356 0.0071 0.0057 0.0027 0.0218 0.0128 0.0089 0.0035 0.0007 0.0005 0.0024 0.0043 0.0089 0.0029 0.0111 0.005 0.0066 0.012
Golgi 0.0134 0.0008 0.007 0.0043 0.0112 0.0021 0.0012 0.0003 0.0002 0.0002 0.0005 0.0009 0.0043 0.0002 0.0153 0.0052 0.0007 0.0068
Lipid Particles 0.047 0.0125 0.017 0.0158 0.035 0.0165 0.0175 0.0019 0.0029 0.0014 0.0082 0.0201 0.0124 0.0014 0.0205 0.1221 0.018 0.0196
Mitochondria 0.0069 0.0002 0.0052 0.0368 0.0128 0.0021 0.0003 0.0001 0 0.0001 0.0002 0.001 0.0165 0.0002 0.0009 0.0136 0.0005 0.0036
None 0.0376 0.1192 0.0008 0.0002 0.0636 0.0465 0.0267 0.0184 0.0004 0.0043 0.0055 0.0044 0.1454 0.175 0.02 0.0034 0.1585 0.1358
Nuclear Periphery 0.024 0.004 0.0104 0.0012 0.0478 0.0206 0.0171 0.0189 0.0019 0.0037 0.0161 0.0116 0.0024 0.0099 0.0076 0.0116 0.0647 0.0063
Nucleolus 0.0079 0.0463 0.0076 0.0062 0.0468 0.0441 0.0266 0.0827 0.0564 0.0287 0.0554 0.0657 0.0103 0.0174 0.0326 0.0456 0.0576 0.0394
Nucleus 0.0432 0.0648 0.0171 0.0043 0.1861 0.1281 0.1248 0.1917 0.0418 0.0413 0.0904 0.186 0.0368 0.1746 0.0062 0.16 0.1343 0.1591
Peroxisomes 0.0108 0.0017 0.0051 0.0286 0.0192 0.0046 0.0031 0.0002 0.0001 0.0005 0.0006 0.0034 0.0012 0.0002 0.0098 0.0135 0.001 0.0058
Punctate Nuclear 0.4944 0.6291 0.8634 0.8189 0.4631 0.6077 0.709 0.6609 0.8885 0.9124 0.7785 0.6644 0.6678 0.5888 0.8065 0.5733 0.4992 0.543
Vacuole 0.0055 0.0013 0.0006 0.0001 0.0058 0.0015 0.0016 0.0007 0.0001 0.0001 0.0003 0.0006 0.001 0.0006 0.0004 0.0009 0.0008 0.0017
Vacuole Periphery 0.0028 0.0001 0.0012 0.0001 0.0066 0.0012 0.0004 0.0002 0 0.0001 0.0001 0.0002 0.0006 0.0001 0.0002 0.0015 0.0011 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.4188 10.053 3.7299 5.8837 9.4345 9.9172 13.4531 12.7119 6.4775 12.8016
Translational Efficiency 0.6849 0.8034 0.6502 0.3452 0.5654 0.6828 0.4629 0.3542 0.5659 0.4132

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1435 943 94 379 1700 1625 275 587 3135 2568 369 966

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 602.27 678.82 1012.10 776.09 684.05 691.01 915.00 786.63 646.62 686.53 939.74 782.49
Standard Deviation 66.77 88.59 129.50 126.12 78.95 84.72 114.48 117.48 84.15 86.36 125.81 121.05
Intensity Change Log 2 — 0.172619 0.748870 0.365814 — 0.014605 0.419670 0.201583 — 0.090747 0.583182 0.280810

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000178 0.000427 0.002754 0.000534 0.000303 0.000377 0.001205 0.000627 0.000246 0.000395 0.001600 0.000591
Bud Neck 0.012047 0.009831 0.005222 0.005430 0.013227 0.020025 0.003343 0.008056 0.012687 0.016282 0.003821 0.007026
Bud Site 0.005436 0.008266 0.012624 0.006949 0.005199 0.007188 0.011475 0.007986 0.005307 0.007584 0.011768 0.007579
Cell Periphery 0.000461 0.000243 0.000281 0.000235 0.000266 0.000181 0.000083 0.000307 0.000355 0.000204 0.000133 0.000279
Cytoplasm 0.338032* 0.136679* 0.116525* 0.164415* 0.229288* 0.172646* 0.004619 0.072826 0.279064* 0.159439* 0.033126 0.108760*
Cytoplasmic Foci 0.258520* 0.198206* 0.021579 0.049986 0.125220 0.157827 0.003429 0.018346 0.186236* 0.172655 0.008053 0.030760
Eisosomes 0.000154 0.000127 0.000079 0.000076 0.000082 0.000106 0.000043 0.000086 0.000115 0.000113 0.000052 0.000082
Endoplasmic Reticulum 0.000875 0.001197 0.005828 0.003945 0.001125 0.000977 0.002724 0.001624 0.001011 0.001058 0.003515 0.002535
Endosome 0.011628 0.015437 0.008873 0.016171 0.008924 0.020785 0.002248 0.004821 0.010162 0.018821 0.003935 0.009274
Golgi 0.004275 0.006867 0.003467 0.008350 0.002746 0.006884 0.000720 0.002058 0.003446 0.006878 0.001420 0.004527
Lipid Particles 0.046255 0.039129 0.005393 0.003640 0.021100 0.031791 0.001075 0.004731 0.032614 0.034486 0.002175 0.004303
Mitochondria 0.012379 0.022030 0.000526 0.002918 0.012007 0.024027 0.000594 0.004465 0.012177 0.023294 0.000576 0.003858
Mitotic Spindle 0.024389 0.280212* 0.385986* 0.420892* 0.201424* 0.247180* 0.761132* 0.397690* 0.120389* 0.259310* 0.665567* 0.406793*
None 0.040516 0.026468 0.005097 0.026176 0.035891 0.025370 0.002706 0.018355 0.038008 0.025773 0.003316 0.021423
Nuclear Periphery 0.001487 0.001700 0.000809 0.002217 0.002741 0.002239 0.000933 0.001160 0.002167 0.002041 0.000901 0.001575
Nuclear Periphery Foci 0.007373 0.025810 0.086969 0.006112 0.025670 0.010568 0.027203 0.003950 0.017295 0.016165 0.042428 0.004798
Nucleolus 0.032690 0.064215 0.021780 0.013897 0.108269 0.053309 0.056108 0.053782 0.073674 0.057314 0.047363 0.038133
Nucleus 0.178973* 0.128419 0.268007* 0.213210* 0.190680* 0.177365* 0.114451 0.385596* 0.185321* 0.159392* 0.153569* 0.317962*
Peroxisomes 0.014423 0.025682 0.017754 0.003007 0.011927 0.021375 0.003248 0.004453 0.013070 0.022957 0.006943 0.003886
Vacuole 0.009046 0.007619 0.029280 0.044777 0.003238 0.017178 0.002479 0.008409 0.005896 0.013668 0.009307 0.022678
Vacuole Periphery 0.000862 0.001435 0.001167 0.007062 0.000672 0.002601 0.000182 0.000672 0.000759 0.002173 0.000433 0.003179

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.49 -6.45 -8.85 -5.57 5.17 -1.87 -7.07 -6.61 -5.31 4.38 -4.78 -9.47 -10.03 -7.24 6.54
Bud Neck 1.80 -0.84 -0.76 -2.58 0.42 -4.49 0.09 -0.66 2.75 -0.66 -3.19 -0.34 -0.92 1.69 -0.47
Bud Site -0.80 -5.23 -4.31 -3.47 2.68 -2.40 -2.67 -3.68 -2.66 0.20 -2.18 -4.57 -5.46 -4.23 1.16
Cell Periphery 2.42 -1.18 2.18 -0.66 4.30 1.29 1.47 -1.81 -2.53 -2.63 2.77 0.85 0.94 -2.59 0.13
Cytoplasm 17.77 9.60 11.94 -1.13 -1.09 -0.76 23.37 9.29 9.86 -8.88 11.61 25.09 15.84 6.27 -7.25
Cytoplasmic Foci 10.81 33.95 32.54 18.18 -1.68 -2.48 28.09 23.12 25.34 -4.17 6.76 44.77 38.82 30.98 -5.21
Eisosomes 2.65 -0.87 -0.39 -1.90 0.57 -3.37 -0.50 -1.64 0.30 -0.62 0.69 0.26 -0.88 -1.25 -0.77
Endoplasmic Reticulum -4.23 -6.79 -9.13 -8.29 3.10 -0.55 -7.15 -7.53 -7.29 4.01 -3.28 -9.71 -11.69 -10.87 4.34
Endosome -1.60 -0.69 -0.89 0.06 -0.13 -7.16 6.07 4.41 9.64 -1.44 -6.88 4.90 2.05 7.08 -2.03
Golgi -3.62 -0.50 0.84 2.53 1.04 -4.12 6.58 4.40 6.14 0.41 -5.13 4.03 2.79 5.79 0.52
Lipid Particles 1.89 12.43 14.35 11.41 1.49 -5.87 15.31 11.02 13.54 -2.14 -1.64 19.20 17.72 17.72 -1.50
Mitochondria -1.92 7.27 6.16 6.37 -2.08 -5.89 7.95 3.41 7.92 -3.01 -5.60 10.50 6.52 10.17 -3.51
Mitotic Spindle -20.34* -6.47 -14.64 -2.88 -1.67 -2.79 -14.90 -9.32 -7.45 6.30 -15.19 -17.32 -17.04 -7.66 4.97
None 5.40 11.36 7.47 3.33 -1.56 3.29 19.81 7.08 3.93 -4.13 6.46 21.62 10.46 5.11 -4.42
Nuclear Periphery -1.21 -1.49 -5.03 -4.24 -2.81 1.54 3.47 3.90 2.16 0.26 0.07 1.07 -1.80 -1.74 -2.34
Nuclear Periphery Foci -5.84 -6.19 -0.23 4.72 6.11 8.03 1.09 12.19 4.89 5.56 2.81 -4.55 9.46 6.63 7.68
Nucleolus -3.58 3.26 5.35 7.81 0.58 13.75* 8.85 10.36 0.02 0.21 8.30 6.95 10.51 4.06 1.19
Nucleus 5.05 -2.70 -7.08 -9.29 -1.20 -3.14 -2.69 -12.54 -10.90 -6.85 0.29 -3.42 -14.21 -14.06 -6.39
Peroxisomes -5.31 0.06 6.51 9.47 1.41 -6.70 4.65 6.27 11.36 0.94 -8.29 2.59 9.20 14.75 1.68
Vacuole 1.68 -4.80 -8.61 -8.76 -2.77 -8.22 -3.99 -4.48 -1.20 -2.88 -6.50 -5.14 -9.31 -7.80 -5.21
Vacuole Periphery -1.40 -0.79 -1.64 -1.45 -1.44 -2.71 -0.03 -1.06 2.25 -1.06 -2.84 0.03 -1.79 0.08 -1.78
Standard name
Human Ortholog
Description Kinesin-related motor protein; required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning; functionally redundant with Cin8p for chromosomal but not plasmid functions
Localization
Cell Percentages spindle pole (22%), nucleus (16%), mixed (17%)
Cell Cycle Regulation Yes
spindle pole - S/G2 (4.6e-15)
nucleus - T/G1 (6.4e-10)
Subcompartmental Group N/A

Kip1

Kip1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kip1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available