Standard name
Description Histone acetyltransferase catalytic subunit of NuA3 complex; acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0.05 0 0.08 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0.14 0.25 0.19 0.23 0.19 0.21 – 0.14 0.09 0.13 0.1 0.17 0.21 0.28 0.25 0.24 0.28 0 0 0.05 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0.1 0.07 0.07 0.09 0 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.28 0.14 0.06 0.2 0.12 0.42 0.55 – 0.7 0.7 0.76 0.51 0.31 0.08 0.07 0.15 0.09 0.15 0 0 0.08 0 0.06 0
Nucleus 0.72 0.67 0.53 0.57 0.58 0.49 0.44 – 0.47 0.42 0.38 0.51 0.51 0.58 0.51 0.52 0.54 0.54 0.49 0.47 0.32 0.35 0.3 0.35
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.11 0.13 0.08 0.1 0.12 0.12
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.05 0.06 0.14 0.17 0.09 0 0.09 – 0 0.05 0.08 0.08 0 0.06 0.05 0.09 0.09 0.14 0.11 0.09 0.11 0.09 0.12 0.13
Vac/Vac Membrane 0 0.06 0 0 0.06 0.07 0 – 0 0 0 0 0 0 0.05 0.06 0.1 0 0.13 0.14 0.23 0.26 0.2 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 3 2 1
Bud 0 0 0 1 1 1 5 – 4 7 7 14 0 0 0 4 0 2 1 6 2 4 11 10
Bud Neck 1 0 18 0 7 3 5 – 2 3 3 8 0 0 2 2 1 6 1 3 4 5 13 12
Bud Site 1 0 0 0 0 3 2 – 11 7 20 15 2 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 1 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 12 47 45 23 41 32 40 – 31 22 31 32 67 85 144 110 61 69 8 15 12 9 16 7
Endoplasmic Reticulum 0 0 0 0 0 2 1 – 0 0 0 1 0 1 1 16 13 14 0 0 1 1 2 5
Endosome 0 11 4 4 5 2 2 – 3 0 0 8 6 39 39 32 24 3 11 19 8 10 18 17
Golgi 0 2 0 0 0 0 0 – 0 0 0 0 0 1 1 0 0 1 0 2 0 0 5 0
Mitochondria 51 45 10 24 21 70 106 – 156 172 185 158 121 34 35 64 22 37 7 19 20 10 27 18
Nucleus 131 220 96 68 105 82 84 – 105 104 93 158 196 237 267 230 140 132 121 213 83 100 140 174
Nuclear Periphery 0 2 0 0 0 0 0 – 0 1 1 0 0 0 0 2 0 0 1 1 0 0 2 3
Nucleolus 0 4 0 0 0 0 1 – 1 1 6 1 14 7 6 3 1 0 27 60 21 28 56 59
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 3 0 0 0 0 0 0 1 1 2
SpindlePole 10 20 26 21 17 7 18 – 10 12 19 24 17 23 28 38 23 34 28 40 29 26 56 64
Vac/Vac Membrane 7 20 4 3 10 12 2 – 2 8 4 6 15 13 24 27 25 7 32 64 58 73 96 93
Unique Cell Count 182 326 180 120 181 168 193 224 246 244 312 386 409 523 439 257 246 250 456 257 283 473 493
Labelled Cell Count 213 371 204 144 207 215 266 326 337 369 425 438 440 550 528 310 306 250 456 257 283 473 493


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 4.4 4.9 4.6 5.1 3.7 4.1 – 3.6 3.5 3.4 3.9 4.0 4.1 4.4 5.7 6.5 5.0 4.1 4.4 4.4
Std Deviation (1e-4) 0.4 0.7 1.3 1.5 1.5 0.9 1.6 – 1.3 1.3 1.2 1.0 0.9 1.0 1.2 1.1 1.7 0.9 1.3 1.5 1.5
Intensity Change (Log2) – – – -0.1 0.07 -0.41 -0.25 – -0.47 -0.47 -0.51 -0.32 -0.3 -0.25 -0.16 0.2 0.4 0.02 -0.25 -0.17 -0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 –
Bud Neck – – – – – – – – – – – – 0 0 –
Bud Site – – – – – – – – – – – – 0 0 –
Cell Periphery – – – – – – – – – – – – 0 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 –
Endosome – – – – – – – – – – – – 0 0 –
Golgi – – – – – – – – – – – – 0 0 –
Mitochondria – – – – – – – – – – – – 0 0 –
Nuclear Periphery – – – – – – – – – – – – 0 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 –
Peroxisomes – – – – – – – – – – – – 0 0 –
SpindlePole – – – – – – – – – – – – 0 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 –
Cytoplasm – – – – – – – – – – – – 0 0 –
Nucleus – – – – – – – – – – – – 0 0 –
Vacuole – – – – – – – – – – – – 2.0 3.1 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2423 2.3097 2.4562 2.1024 2.4958 1.912 0.5173 0.0692 0.0017 -0.5017 0.2551 0.008 -0.5246 -0.2291 -0.3675 0.0832 -0.492 -0.458
Actin 0.0968 0.0008 0.014 0.0382 0.0169 0.0023 0.0296 0.0003 0.0206 0.0001 0.034 0.0018 0.0089 0.0002 0.0038 0.0237 0.0103 0.0004
Bud 0.0009 0.0003 0.0002 0.0004 0.0007 0.0001 0.0013 0.0008 0.0004 0 0.0005 0.0002 0.0001 0.0001 0.0001 0.0004 0.0001 0.0003
Bud Neck 0.0099 0.0012 0.0008 0.0013 0.0014 0.0121 0.0088 0.0028 0.0021 0.0008 0.0019 0.0058 0.0126 0.0011 0.0007 0.0218 0.002 0.0075
Bud Periphery 0.0017 0.0002 0.0002 0.0007 0.0009 0.0002 0.0027 0.0005 0.0006 0 0.0011 0.0005 0.0002 0.0001 0.0001 0.0007 0.0002 0.0002
Bud Site 0.0054 0.0029 0.0029 0.0018 0.003 0.0003 0.014 0.0111 0.0023 0.0002 0.0008 0.0004 0.0012 0.0016 0.0003 0.0118 0.0004 0.0001
Cell Periphery 0.001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0006 0.0001 0.0001 0 0.0001 0.0001 0.0001 0 0 0.0004 0.0001 0
Cytoplasm 0.0724 0.0871 0.0616 0.0552 0.0334 0.0581 0.0391 0.0511 0.0305 0.0182 0.0384 0.0267 0.0411 0.0834 0.0542 0.0301 0.0315 0.0324
Cytoplasmic Foci 0.0249 0.004 0.0199 0.0184 0.0714 0.0041 0.0165 0.0068 0.0097 0.0005 0.0069 0.0047 0.0048 0.002 0.0032 0.0023 0.0038 0.0031
Eisosomes 0.0015 0.0001 0.0003 0.0004 0.0002 0.0001 0.0003 0 0.0001 0 0.0001 0.0001 0.0001 0 0.0004 0.0006 0.0001 0
Endoplasmic Reticulum 0.0183 0.0025 0.0028 0.003 0.0023 0.0015 0.0046 0.0019 0.0025 0.0007 0.002 0.001 0.0028 0.0017 0.0017 0.0013 0.0028 0.0005
Endosome 0.0331 0.0021 0.0087 0.017 0.0291 0.0028 0.0235 0.0044 0.0161 0.0008 0.0227 0.0051 0.0084 0.0021 0.0024 0.0053 0.0146 0.0009
Golgi 0.0096 0.0001 0.0025 0.0142 0.0093 0.0002 0.0055 0.0002 0.0059 0 0.0132 0.0023 0.0015 0.0001 0.0005 0.0015 0.0013 0.0001
Lipid Particles 0.0184 0.0003 0.005 0.0261 0.0174 0.0005 0.0147 0.0009 0.0137 0 0.0221 0.0058 0.001 0.0002 0.0004 0.0011 0.0023 0.0001
Mitochondria 0.021 0.0009 0.0012 0.0262 0.01 0.0009 0.0072 0.0004 0.0032 0.0002 0.0165 0.0064 0.0008 0.0004 0.0004 0.0013 0.0012 0.0003
None 0.1705 0.3032 0.2975 0.1363 0.1736 0.1574 0.0683 0.0458 0.0545 0.0199 0.0364 0.0437 0.111 0.1036 0.0957 0.0584 0.0924 0.0519
Nuclear Periphery 0.0312 0.0122 0.0137 0.0448 0.0504 0.0124 0.0322 0.0116 0.0263 0.008 0.0298 0.0173 0.0187 0.0119 0.0138 0.0146 0.0432 0.0058
Nucleolus 0.0159 0.0119 0.0095 0.0046 0.0176 0.0195 0.0151 0.0125 0.0111 0.0162 0.0183 0.0277 0.0102 0.0103 0.0095 0.0114 0.01 0.0156
Nucleus 0.3516 0.4758 0.4731 0.4694 0.3907 0.6022 0.6338 0.7745 0.7102 0.8817 0.6199 0.7433 0.6657 0.678 0.7035 0.7483 0.6034 0.7722
Peroxisomes 0.0155 0.0002 0.0108 0.0577 0.0614 0.0003 0.0119 0.0001 0.0075 0 0.0063 0.0014 0.0006 0.0001 0.0002 0.0008 0.0013 0.0001
Punctate Nuclear 0.0921 0.0924 0.0736 0.081 0.1074 0.1236 0.0597 0.0721 0.0792 0.0513 0.1266 0.1042 0.1086 0.1008 0.1083 0.0621 0.177 0.1081
Vacuole 0.0061 0.0015 0.0011 0.0014 0.0016 0.001 0.0074 0.0016 0.0021 0.001 0.0014 0.001 0.0011 0.002 0.0006 0.0016 0.0012 0.0004
Vacuole Periphery 0.002 0.0002 0.0003 0.0015 0.0012 0.0002 0.0031 0.0003 0.0011 0.0001 0.0012 0.0006 0.0005 0.0003 0.0003 0.0004 0.0008 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.4938 8.694 11.4365 12.2127 12.323 8.3947 13.6852 13.6189 12.3879 13.89
Translational Efficiency 0.4201 0.4821 0.1947 0.285 0.2962 0.3862 0.3028 0.2691 0.2925 0.2318

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘