Standard name
Human Ortholog
Description Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair; forms a complex with Rev3p, Rev7p and Pol32p; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.06 0.05 0.05 0.05 – 0.06 0 0 0.12 0.13 0.16 0.05 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.31 0.24 0.41 0.56 0.64 0.55 – 0.64 0.71 0.73 0 0 0 0.05 0.07 0 0 0 0 0 0 0
Nucleus 0.92 0.93 0.87 0.91 0.8 0.76 0.68 0.71 – 0.69 0.72 0.71 0.87 0.85 0.81 0.9 0.93 0.92 0.86 0.87 0.84 0.8 0.65 0.63
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.08 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0.06 0.13 0.22 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 1 0 1 0
Bud 0 0 1 0 1 4 6 17 – 7 6 4 0 0 0 0 0 0 3 1 1 2 2 3
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 2 0 1 0 0 0 0 0 0 0 0 0
Bud Site 0 0 1 1 0 13 22 15 – 22 20 27 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 11 9 15 16 21 27 35 36 – 35 23 25 32 43 52 21 10 14 1 2 1 1 3 2
Endoplasmic Reticulum 1 0 0 1 0 0 0 0 – 0 0 0 0 2 2 8 10 8 0 0 1 0 3 2
Endosome 0 0 1 0 0 0 0 0 – 0 0 0 0 3 2 0 0 2 3 2 5 0 1 6
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 2 1 0 2 0 0 3 0 2 0 0 1
Mitochondria 10 10 134 73 140 298 439 377 – 401 413 445 2 4 0 23 27 10 4 1 2 0 2 6
Nucleus 230 210 381 278 274 410 467 486 – 434 417 430 230 280 261 417 385 351 225 204 380 116 123 134
Nuclear Periphery 0 1 0 1 3 1 14 6 – 5 3 6 0 0 0 7 4 5 0 0 2 0 0 0
Nucleolus 1 3 5 3 8 17 27 58 – 27 26 26 1 0 0 6 4 2 4 5 11 1 2 4
Peroxisomes 1 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 1 2 0 4 3 5 – 3 3 0 1 4 3 4 1 2 2 1 6 0 1 3
Vac/Vac Membrane 9 11 2 1 7 8 12 8 – 9 14 10 2 2 4 22 16 14 9 10 29 19 42 44
Unique Cell Count 250 227 438 306 343 536 683 686 631 582 607 264 331 321 462 413 382 263 236 453 145 190 215
Labelled Cell Count 264 244 541 376 454 782 1025 1008 943 925 973 272 340 326 511 457 408 263 236 453 145 190 215


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 5.8 4.1 4.3 4.0 3.9 3.6 3.6 – 3.3 3.3 3.3 6.1 6.2 6.2 7.0 7.0 7.4 6.5 7.0 6.8
Std Deviation (1e-4) 0.9 0.8 0.8 1.3 1.2 1.2 1.6 1.4 – 0.9 1.0 0.8 1.3 1.3 1.4 1.4 1.3 1.5 1.4 1.7 1.6
Intensity Change (Log2) – – – 0.04 -0.06 -0.1 -0.22 -0.21 – -0.33 -0.33 -0.34 0.57 0.57 0.57 0.77 0.75 0.83 0.66 0.75 0.72


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 1.2 1.8 1.2 1.3 1.4 – 1.6 0.4 0.6 4.5 5.0 6.1 0.9 -0.9 0.2
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 – 0 0 0 -9.7 -10.5 -10.9 -10.1 -9.0 -10.5
Nucleus 1.6 -2.7 -4.2 -7.1 -6.3 – -6.9 -5.9 -6.2 0.1 -0.9 -2.1 1.5 3.0 2.3
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0.2 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 1.9 0 – 0 2.5 1.8 0 0 0 4.0 3.5 3.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8306 1.9086 1.9131 1.8536 1.3312 1.53 3.536 3.8242 3.8103 3.4239 2.9366 3.2777 0.0652 0.4978 0.2059 0.3483 0.8321 0.1577
Actin 0.0124 0 0.0044 0 0.001 0.0001 0.0286 0.0001 0.0089 0.0086 0.0027 0.0001 0.0082 0 0.0074 0 0.0017 0.0003
Bud 0.0002 0 0.0001 0 0.0002 0.0001 0.0013 0.0001 0.0003 0.001 0.0017 0.0002 0.0005 0 0.0001 0 0.0003 0.0005
Bud Neck 0.0039 0 0.0004 0 0.0004 0.0003 0.0081 0.0004 0.0058 0.0007 0.0011 0.0007 0.0007 0.0001 0.0001 0 0.0003 0.0017
Bud Periphery 0.0005 0 0.0002 0 0.0007 0.0002 0.0037 0 0.0007 0.0009 0.0048 0.0011 0.0004 0 0.0001 0 0.0001 0.0005
Bud Site 0.001 0 0.0029 0 0.0003 0.0001 0.005 0.0036 0.0026 0.0069 0.0013 0.0001 0.0249 0.0001 0.0003 0 0.0011 0.0018
Cell Periphery 0.0001 0 0.0001 0 0.0001 0 0.0016 0 0.0003 0.0002 0.0006 0.0001 0.0005 0 0 0 0 0.0001
Cytoplasm 0.0018 0.0001 0.0002 0.0002 0.0008 0.0001 0.0058 0.0004 0.0112 0.005 0.0027 0.0001 0.0468 0.0004 0.0067 0.0001 0.1256 0.0041
Cytoplasmic Foci 0.003 0 0.0002 0 0.0021 0 0.004 0.0064 0.0023 0.0076 0.0005 0 0.0128 0 0.0187 0 0.0579 0.0192
Eisosomes 0.0001 0 0.0001 0 0.0001 0 0.0005 0 0.0002 0.0002 0.0001 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.002 0 0.0002 0 0.0013 0 0.0081 0.0004 0.0018 0.0009 0.0068 0.0001 0.0047 0 0.0124 0 0.0129 0.0006
Endosome 0.0073 0 0.0011 0 0.0148 0.0001 0.0163 0.0015 0.0078 0.0089 0.0112 0.0001 0.0142 0 0.0145 0 0.1799 0.033
Golgi 0.0022 0 0.0007 0 0.004 0 0.006 0.0004 0.0024 0.0059 0.0014 0 0.0017 0 0.0028 0 0.0266 0.0043
Lipid Particles 0.0032 0 0.0029 0 0.0029 0.0001 0.0052 0.0005 0.0046 0.0009 0.006 0.0001 0.0016 0 0.0033 0 0.0053 0.0025
Mitochondria 0.004 0 0.0021 0.0001 0.0274 0.002 0.0148 0.0002 0.0096 0.0026 0.0201 0.0011 0.0014 0.0002 0.0011 0.0001 0.009 0.0009
None 0.0102 0 0.0001 0.0004 0.0007 0 0.0067 0.0004 0.0052 0.0429 0.0006 0.0001 0.0248 0 0.0011 0 0.0017 0.0002
Nuclear Periphery 0.0088 0.001 0.0016 0.0012 0.01 0.0025 0.0215 0.0087 0.0278 0.0008 0.0301 0.0011 0.0178 0.0022 0.0094 0.0011 0.0229 0.0014
Nucleolus 0.0147 0.0108 0.0105 0.0103 0.0221 0.0177 0.0182 0.0199 0.0053 0.0031 0.022 0.019 0.0061 0.0122 0.0104 0.0047 0.0041 0.0119
Nucleus 0.9041 0.9866 0.9704 0.9661 0.8939 0.9747 0.8176 0.9533 0.8938 0.8943 0.854 0.9734 0.8007 0.9839 0.905 0.9937 0.5299 0.908
Peroxisomes 0.0067 0 0.0003 0 0.0003 0 0.0018 0.0014 0.0021 0.0012 0.0005 0.0001 0.0009 0 0.0019 0 0.0084 0.0007
Punctate Nuclear 0.0118 0.0013 0.0008 0.0216 0.0134 0.0012 0.0121 0.0017 0.0049 0.0059 0.018 0.0015 0.0287 0.0005 0.0041 0.0001 0.0053 0.0031
Vacuole 0.0013 0 0.0002 0 0.0017 0.0002 0.01 0.0005 0.0014 0.0012 0.0063 0.0006 0.0016 0.0002 0.0003 0 0.0041 0.0047
Vacuole Periphery 0.0008 0 0.0002 0 0.0017 0.0004 0.003 0.0002 0.001 0.0004 0.0075 0.0002 0.0007 0.0001 0.0004 0 0.0027 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.1034 8.0851 12.9988 18.5194 18.0781 16.572 13.795 15.6327 18.7203 29.5316
Translational Efficiency 1.2102 1.5899 0.7634 0.8593 1.0322 1.7137 1.1015 0.6285 0.7318 0.6819

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2249 1912 162 998 1508 1664 1831 2043 3757 3576 1993 3041

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 695.46 820.22 970.99 901.22 670.29 777.64 954.76 1022.29 685.36 800.41 956.08 982.56
Standard Deviation 84.39 116.22 115.93 145.34 112.43 102.89 118.53 132.37 97.42 112.24 118.41 148.11
Intensity Change Log 2 — 0.238043 0.481489 0.373912 — 0.214317 0.510353 0.608947 — 0.226448 0.495727 0.494048

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000061 0.000274 0.001056 0.002763 0.000181 0.000273 0.000476 0.000450 0.000109 0.000274 0.000523 0.001209
Bud Neck 0.002743 0.004878 0.002494 0.011255 0.001873 0.005794 0.013638 0.022702 0.002394 0.005304 0.012732 0.018945
Bud Site 0.000497 0.001096 0.000795 0.007402 0.000768 0.000978 0.001068 0.002633 0.000606 0.001041 0.001046 0.004198
Cell Periphery 0.000110 0.000129 0.000092 0.000271 0.000188 0.000090 0.000062 0.000072 0.000141 0.000111 0.000065 0.000137
Cytoplasm 0.044736 0.009702 0.004474 0.037962 0.042336 0.008674 0.001399 0.005681 0.043773 0.009224 0.001649 0.016275
Cytoplasmic Foci 0.002949 0.001621 0.000607 0.003362 0.006276 0.001549 0.000017 0.000195 0.004284 0.001587 0.000065 0.001235
Eisosomes 0.000012 0.000014 0.000053 0.000035 0.000025 0.000019 0.000020 0.000018 0.000017 0.000016 0.000023 0.000024
Endoplasmic Reticulum 0.001049 0.004527 0.010246 0.007522 0.002336 0.004670 0.005050 0.006193 0.001566 0.004594 0.005472 0.006629
Endosome 0.000279 0.000804 0.000563 0.006038 0.000612 0.000655 0.000170 0.000838 0.000413 0.000734 0.000202 0.002545
Golgi 0.000095 0.000996 0.000079 0.002010 0.000202 0.000376 0.000037 0.000797 0.000138 0.000707 0.000040 0.001195
Lipid Particles 0.000522 0.000815 0.000690 0.000945 0.001578 0.000680 0.000054 0.000100 0.000946 0.000752 0.000106 0.000377
Mitochondria 0.001611 0.002772 0.000683 0.006667 0.000902 0.004094 0.001133 0.002056 0.001326 0.003387 0.001096 0.003569
Mitotic Spindle 0.000935 0.001023 0.000093 0.041911 0.001054 0.006421 0.001197 0.009206 0.000983 0.003535 0.001107 0.019939
None 0.012643 0.006768 0.001484 0.022025 0.037325 0.003988 0.001305 0.003353 0.022550 0.005474 0.001319 0.009481
Nuclear Periphery 0.000834 0.001189 0.000561 0.001734 0.000655 0.001435 0.000594 0.002633 0.000762 0.001304 0.000592 0.002338
Nuclear Periphery Foci 0.000364 0.000583 0.000376 0.002376 0.001379 0.000724 0.000109 0.000721 0.000771 0.000649 0.000131 0.001264
Nucleolus 0.011731 0.010554 0.004778 0.003998 0.015455 0.010295 0.003470 0.004120 0.013226 0.010434 0.003576 0.004080
Nucleus 0.917665* 0.949630* 0.963451* 0.819203* 0.884696* 0.945537* 0.968530* 0.931978* 0.904432* 0.947725* 0.968117* 0.894967*
Peroxisomes 0.000274 0.000686 0.000362 0.001041 0.000527 0.001754 0.000080 0.000088 0.000375 0.001183 0.000103 0.000401
Vacuole 0.000796 0.001395 0.006915 0.017999 0.001479 0.001574 0.001473 0.004315 0.001070 0.001478 0.001916 0.008806
Vacuole Periphery 0.000094 0.000546 0.000147 0.003481 0.000154 0.000420 0.000119 0.001851 0.000118 0.000487 0.000121 0.002386

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.26 -11.26 -3.15 -2.79 -1.49 -1.72 -12.29 -12.71 -6.26 -1.51 -4.57 -18.49 -4.74 -3.89 -2.30
Bud Neck -3.29 -0.45 -12.03 -9.34 -12.01 -5.33 -13.54 -18.19 -13.75 -5.53 -5.84 -12.96 -20.70 -16.66 -5.09
Bud Site -2.08 -2.52 -8.13 -7.11 -7.63 -0.60 -1.85 -4.35 -4.21 -3.58 -2.12 -3.66 -9.01 -7.69 -7.38
Cell Periphery -1.18 0.80 -3.74 -2.71 -4.16 5.42 7.28 6.77 2.42 -1.31 2.61 7.82 2.55 -0.07 -5.32
Cytoplasm 13.98 14.58 3.71 -6.83 -7.82 11.75 14.99 12.89 1.93 -7.30 18.34 23.76 14.32 -4.06 -11.54
Cytoplasmic Foci 2.72 5.41 2.98 0.12 -2.42 4.82 6.54 6.43 4.12 -2.98 5.53 9.41 8.07 3.52 -4.73
Eisosomes -0.99 -9.92 -15.93 -15.26 1.41 2.96 2.06 2.09 -1.84 -0.02 1.60 -5.29 -9.81 -12.56 -5.04
Endoplasmic Reticulum -12.65 -12.66 -20.90 -10.45 1.44 -6.90 -9.51 -12.33 -5.04 -3.60 -14.30 -16.39 -23.58 -9.92 -5.50
Endosome -2.30 -2.18 -4.62 -3.58 -4.04 0.29 3.51 0.38 0.14 -3.32 -1.96 3.53 -4.33 -2.83 -5.54
Golgi -2.01 0.71 -4.11 0.10 -4.40 -0.95 4.65 -0.90 -0.34 -1.66 -2.23 3.36 -2.49 0.25 -3.14
Lipid Particles -0.74 -0.89 -1.63 -0.10 -0.83 2.05 3.63 3.47 4.61 -4.51 0.82 4.77 3.24 1.96 -4.46
Mitochondria -1.40 1.69 -2.52 -1.38 -3.71 -3.85 -1.94 -5.37 2.34 -4.39 -3.41 0.23 -3.37 0.78 -4.60
Mitotic Spindle -0.14 1.58 -7.76 -7.75 -7.99 -3.11 -0.64 -4.87 -1.64 -4.64 -2.90 -0.84 -9.13 -7.42 -8.68
None 4.21 10.21 -0.02 -2.91 -6.09 10.59 11.68 10.93 0.90 -4.74 11.25 15.06 10.38 -1.08 -7.53
Nuclear Periphery -1.89 1.67 -9.00 -5.97 -10.81 -3.24 -0.45 -4.80 -2.54 -4.78 -3.59 0.61 -6.66 -4.29 -6.97
Nuclear Periphery Foci -1.05 0.56 -3.31 -2.90 -3.49 1.26 3.18 1.54 0.36 -2.28 0.51 3.78 -1.20 -1.70 -4.30
Nucleolus 1.62 5.69 5.57 4.42 -0.83 3.59 7.34 6.47 3.43 -1.30 3.47 8.82 8.09 5.32 -1.21
Nucleus -7.14 -8.68 8.93 13.08 13.97 -9.70 -13.69 -6.60 4.32 9.74 -11.75 -16.73 1.23 12.17 16.65
Peroxisomes -1.61 -0.44 -1.90 0.38 -1.75 -2.37 4.99 5.03 3.64 0.24 -2.96 5.61 2.58 3.73 -2.98
Vacuole -2.40 -4.70 -9.19 -8.94 -5.68 -0.01 -0.46 -5.70 -5.83 -5.37 -1.91 -3.61 -10.94 -10.53 -9.49
Vacuole Periphery -3.10 -2.79 -2.65 -1.77 -2.54 -2.68 1.26 -2.16 -1.52 -2.24 -4.08 -0.35 -3.28 -2.14 -3.26
Standard name
Human Ortholog
Description Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair; forms a complex with Rev3p, Rev7p and Pol32p; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Pol31

Pol31


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pol31-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available