Standard name
Human Ortholog
Description Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.13 0.13 0.09 0.09 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 0 0.15 0.2 0.12 0.16 0.31 0.22 0.22 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.94 0.95 0.95 0.94 0.94 0.88 0.84 0.84 0.84 0.74 0.83 0.8 0.89 0.83 0.82 0.81 0.79 0.78 0.93 0.94 0.93 0.91 0.85 0.77
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.07 0.11 0.16 0.13 0.16 0.18 0.22 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.1 0.13 0 0 0 0 0.1 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 4 1 3 10 4 2 0 0 0 0 0 0 2 2 4 2 0 2
Bud Neck 0 0 0 0 1 0 0 3 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1 3 4 10 5 15 13 17 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 6 6 11 13 8 14 21 10 24 12 5 20 45 46 22 25 23 0 0 0 0 0 3
Endoplasmic Reticulum 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 2 7 1 0 0 0 0 0 0
Endosome 1 0 1 0 1 0 0 1 1 1 0 0 0 4 8 2 0 4 2 0 0 0 2 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 1 0 3 0 1 1
Mitochondria 12 0 4 11 16 68 140 90 104 235 133 172 0 1 0 4 3 3 0 0 2 0 0 4
Nucleus 197 169 353 265 400 406 604 628 551 557 501 634 178 280 298 199 220 221 196 170 350 94 254 274
Nuclear Periphery 1 0 0 2 0 4 8 6 6 4 3 10 0 0 5 0 1 1 0 0 0 0 0 1
Nucleolus 3 2 9 5 12 33 81 116 83 123 107 174 0 3 4 4 4 4 0 2 2 1 4 13
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
SpindlePole 1 1 0 0 0 0 1 1 0 1 0 0 0 2 1 0 0 0 1 0 0 0 2 4
Vac/Vac Membrane 1 2 7 2 7 5 11 11 8 11 2 4 2 13 12 25 27 37 3 4 8 1 30 45
Unique Cell Count 209 178 372 283 425 459 717 748 657 751 605 796 201 339 365 245 277 285 211 182 377 104 299 357
Labelled Cell Count 222 180 380 296 451 529 867 888 771 982 775 1018 201 348 376 258 288 294 211 182 377 104 299 357


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 8.2 7.4 6.7 6.6 5.7 5.6 5.6 5.6 5.0 5.2 5.2 8.7 8.6 8.0 11.5 11.6 11.2 7.3 8.3 8.8
Std Deviation (1e-4) 1.1 1.4 1.3 1.5 1.4 1.3 1.3 1.4 1.2 1.4 1.5 1.2 1.5 1.6 1.5 2.3 2.6 2.6 1.3 1.1 1.5
Intensity Change (Log2) – – – -0.14 -0.17 -0.39 -0.42 -0.4 -0.4 -0.57 -0.51 -0.52 0.23 0.21 0.1 0.64 0.65 0.6 -0.03 0.15 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 2.8 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.8 1.3 0.1 0.4 1.2 -0.1 1.5 0.4 0 4.6 6.0 5.8 4.3 4.4 4.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.4 2.4 7.0 8.5 6.2 7.4 11.6 9.1 9.1 0 0 0 0 0 0
Nucleus -0.7 -0.5 -3.3 -5.1 -5.2 -5.2 -8.3 -5.5 -6.7 -2.8 -5.2 -5.6 -5.4 -6.1 -6.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.6 0.4 3.1 5.0 6.6 5.5 6.8 7.2 8.5 0 -1.6 -1.4 -0.7 -0.9 -0.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 -0.3 -1.0 -0.4 -0.5 -0.9 -0.5 0 0 0 1.6 1.2 4.6 4.4 5.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.7607 5.4796 5.4279 4.9687 4.6366 5.2486 3.9488 4.9293 4.6522 4.1852 3.891 4.4009 2.6862 3.6174 3.1304 3.1688 2.7506 2.9133
Actin 0 0 0 0 0 0 0.0163 0 0.003 0.0002 0.0212 0.0016 0.0173 0 0.0051 0 0.0003 0.0031
Bud 0 0 0 0 0 0 0.0012 0 0.0002 0.0002 0.0002 0.0001 0.0004 0 0.0003 0 0.0006 0.0001
Bud Neck 0 0 0 0.0001 0.0004 0.0007 0.0014 0 0.0002 0.0006 0.0003 0.0018 0.0009 0 0.0007 0 0.0006 0.0004
Bud Periphery 0 0 0 0 0 0.0001 0.0022 0 0.0002 0.0011 0.0003 0.0003 0.0008 0 0.0006 0 0.0015 0.0002
Bud Site 0 0 0 0 0.0001 0 0.0031 0 0.0008 0.0004 0.0003 0.0001 0.0009 0 0.0017 0 0.0003 0.0001
Cell Periphery 0 0 0 0 0 0.0001 0.0003 0 0 0.0006 0 0.0001 0.0002 0 0.0002 0 0.0002 0
Cytoplasm 0 0 0.0007 0.0002 0.0009 0 0.0016 0.0001 0.0007 0.001 0.0003 0.0003 0.0022 0 0.0024 0.0003 0.0004 0.0001
Cytoplasmic Foci 0 0 0 0 0 0 0.0138 0 0.0023 0.0021 0.0012 0.0005 0.0125 0 0.0059 0 0 0.0005
Eisosomes 0 0 0 0 0 0 0.0002 0 0 0 0.0001 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0 0 0 0 0.0001 0 0.0017 0 0.001 0.0002 0.0001 0.0004 0.0022 0 0.0011 0 0.0002 0.0006
Endosome 0 0 0 0 0.0002 0.0001 0.0151 0 0.0077 0.0195 0.0009 0.0022 0.0124 0 0.0091 0 0.0002 0.0029
Golgi 0 0 0 0 0 0 0.0093 0 0.0025 0.0044 0.0029 0.0014 0.0054 0 0.0026 0 0 0.0037
Lipid Particles 0 0 0 0 0 0 0.0195 0 0.0039 0.0053 0.0024 0.0026 0.0143 0 0.0066 0 0 0.0057
Mitochondria 0.0001 0 0.0001 0.0001 0.0002 0.0001 0.0107 0.0001 0.0013 0.0042 0.0056 0.0119 0.0069 0.0001 0.006 0.0001 0.0046 0.002
None 0 0 0.0051 0 0.0001 0 0.0008 0 0.0003 0.0001 0.0001 0.0002 0.0017 0 0.0013 0 0.0001 0.0001
Nuclear Periphery 0.0003 0.0005 0.001 0.001 0.0031 0.0006 0.007 0.0017 0.005 0.0029 0.0016 0.0043 0.0104 0.0003 0.0052 0.0008 0.0029 0.0044
Nucleolus 0.0035 0.0081 0.0028 0.0022 0.0125 0.0191 0.0072 0.0036 0.0066 0.0045 0.0061 0.0191 0.0109 0.0043 0.0056 0.0032 0.0212 0.0158
Nucleus 0.996 0.9907 0.9897 0.9961 0.9805 0.9773 0.8722 0.9943 0.9602 0.9295 0.9457 0.9486 0.8836 0.9945 0.9342 0.9953 0.9634 0.958
Peroxisomes 0 0 0 0 0 0 0.0088 0 0.0006 0.0007 0.0101 0.0007 0.0041 0 0.0022 0 0.0001 0.0004
Punctate Nuclear 0 0.0005 0.0006 0.0002 0.0016 0.0003 0.0031 0 0.0016 0.0003 0.0002 0.002 0.0093 0.0007 0.0041 0.0002 0.0002 0.0009
Vacuole 0 0 0 0 0.0002 0.001 0.003 0 0.001 0.0184 0.0002 0.0009 0.0019 0 0.0031 0 0.0017 0.0004
Vacuole Periphery 0 0 0 0 0.0001 0.0005 0.0016 0.0001 0.0008 0.0037 0.0001 0.0009 0.0015 0 0.002 0 0.0015 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.898 10.4142 11.5917 14.1296 15.1045 11.6434 10.5298 12.4371 16.9589 14.8647
Translational Efficiency 2.4463 2.0359 1.505 1.7661 1.4952 2.4558 1.6298 1.3276 1.2253 1.3737

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
57 176 874 1260 1459 2004 222 2630 1516 2180 1096 3890

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 905.94 1063.38 1041.06 1108.74 850.31 945.70 1173.81 1125.32 852.40 955.20 1067.95 1119.95
Standard Deviation 420.95 158.99 145.83 173.27 119.60 121.26 136.74 180.84 143.32 128.78 153.60 178.59
Intensity Change Log 2 — 0.231170 0.200566 0.291434 — 0.153394 0.465138 0.404274 — 0.194037 0.334724 0.347170

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002614 0.001226 0.000631 0.000952 0.000299 0.000327 0.001217 0.000756 0.000386 0.000400 0.000750 0.000819
Bud Neck 0.020205 0.006956 0.011477 0.024483 0.003960 0.008789 0.005118 0.046948 0.004571 0.008641 0.010189 0.039672
Bud Site 0.017716 0.000873 0.000935 0.005462 0.000633 0.001287 0.000858 0.010431 0.001275 0.001254 0.000919 0.008822
Cell Periphery 0.000659 0.000080 0.000064 0.000132 0.000162 0.000372 0.000056 0.000225 0.000181 0.000349 0.000062 0.000195
Cytoplasm 0.015006 0.001006 0.000936 0.009274 0.001350 0.000773 0.000948 0.026948 0.001863 0.000792 0.000938 0.021223
Cytoplasmic Foci 0.031672 0.000192 0.000021 0.001514 0.000172 0.000713 0.000061 0.001888 0.001356 0.000671 0.000029 0.001767
Eisosomes 0.000136 0.000032 0.000036 0.000028 0.000027 0.000055 0.000057 0.000026 0.000031 0.000053 0.000040 0.000027
Endoplasmic Reticulum 0.021372 0.006007 0.003728 0.006977 0.005357 0.008735 0.008537 0.003602 0.005959 0.008514 0.004702 0.004695
Endosome 0.010787 0.000170 0.000123 0.001508 0.000222 0.001146 0.000206 0.002895 0.000619 0.001067 0.000140 0.002446
Golgi 0.005259 0.000091 0.000040 0.003514 0.000049 0.000719 0.000053 0.001192 0.000245 0.000668 0.000043 0.001944
Lipid Particles 0.033366 0.000124 0.000054 0.000782 0.000064 0.000447 0.000214 0.000245 0.001316 0.000421 0.000086 0.000419
Mitochondria 0.024248 0.001894 0.000888 0.004897 0.000886 0.003503 0.000957 0.005875 0.001764 0.003373 0.000902 0.005558
Mitotic Spindle 0.025698 0.001869 0.000168 0.027114 0.001254 0.004394 0.000126 0.039168 0.002173 0.004190 0.000160 0.035264
None 0.003786 0.000892 0.001530 0.003488 0.001966 0.000826 0.000532 0.010493 0.002035 0.000832 0.001328 0.008224
Nuclear Periphery 0.004934 0.000380 0.000236 0.001848 0.000243 0.000803 0.000336 0.001979 0.000419 0.000769 0.000257 0.001936
Nuclear Periphery Foci 0.009839 0.000169 0.000048 0.001063 0.000210 0.000661 0.000128 0.000717 0.000572 0.000621 0.000065 0.000829
Nucleolus 0.024498 0.004916 0.004887 0.003972 0.007072 0.005997 0.003104 0.004415 0.007727 0.005910 0.004526 0.004272
Nucleus 0.708773* 0.969518* 0.973124* 0.895606* 0.975133* 0.956988* 0.973150* 0.831163* 0.965118* 0.958000* 0.973129* 0.852036*
Peroxisomes 0.018339 0.000886 0.000168 0.000265 0.000154 0.000474 0.000330 0.000807 0.000837 0.000507 0.000200 0.000632
Vacuole 0.015885 0.002429 0.000835 0.005399 0.000557 0.002049 0.003926 0.007555 0.001134 0.002080 0.001461 0.006856
Vacuole Periphery 0.005211 0.000290 0.000071 0.001723 0.000231 0.000941 0.000085 0.002669 0.000418 0.000888 0.000074 0.002363

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.56 3.44 2.50 -0.05 -8.41 -0.68 -11.43 -7.39 -7.26 6.70 -0.09 -9.74 -9.31 -6.73 -0.83
Bud Neck 1.51 1.00 -1.25 -10.81 -10.08 -5.44 -4.36 -21.27 -17.49 -18.72 -4.66 -8.55 -24.44 -19.96 -18.89
Bud Site 2.37 2.36 1.70 -4.54 -4.79 -2.56 -3.03 -10.87 -9.66 -8.06 0.08 0.25 -9.31 -10.28 -10.82
Cell Periphery 1.91 1.96 1.70 -2.85 -8.10 -2.41 2.33 0.32 2.72 -4.03 -2.05 3.21 0.89 2.68 -6.89
Cytoplasm 2.68 2.72 0.61 -7.78 -8.82 1.15 0.14 -16.67 -17.66 -17.19 1.91 1.38 -17.09 -19.33 -19.27
Cytoplasmic Foci 2.81 2.82 2.75 -1.62 -2.21 -2.10 0.92 -3.22 -0.65 -4.00 1.38 2.91 0.99 -0.75 -4.67
Eisosomes 4.45 4.39 4.49 -0.49 1.14 -2.83 -8.98 -0.08 2.86 9.60 -2.38 -5.36 0.50 2.57 9.28
Endoplasmic Reticulum 3.23 3.65 2.99 -1.37 -7.16 -7.29 -6.77 5.15 11.96 9.05 -5.11 1.48 2.79 8.79 1.60
Endosome 1.97 1.98 1.79 -4.04 -4.28 -2.70 -1.75 -3.97 -0.73 -3.56 -1.22 1.98 -2.45 -0.77 -5.20
Golgi 2.32 2.34 1.48 -1.89 -1.95 -1.85 -1.98 -3.63 0.28 -3.41 -1.24 2.18 -2.35 -0.67 -3.09
Lipid Particles 2.66 2.66 2.61 -1.99 -2.21 -2.27 -5.43 -6.01 1.16 1.93 1.72 2.38 1.86 0.22 -2.77
Mitochondria 1.87 1.97 1.66 -1.79 -5.13 -2.88 -1.05 -7.01 -1.25 -7.41 -1.69 1.40 -4.44 -1.45 -9.33
Mitotic Spindle 1.51 1.76 -0.11 -4.89 -7.19 -2.43 -0.57 -12.41 -10.72 -8.88 -1.63 1.42 -13.19 -11.75 -14.27
None 2.49 1.97 -0.09 -5.47 -4.22 2.29 2.81 -9.04 -12.75 -13.16 2.50 1.34 -8.35 -13.90 -11.97
Nuclear Periphery 1.29 1.32 0.46 -8.80 -11.73 -4.11 -4.70 -16.67 -9.52 -13.89 -2.02 0.50 -13.48 -11.66 -19.63
Nuclear Periphery Foci 2.24 2.27 1.97 -3.84 -4.36 -1.35 0.60 -2.22 -0.07 -4.16 -0.16 2.36 -1.11 -0.81 -6.56
Nucleolus 3.64 3.78 3.45 -0.90 -1.56 1.13 4.53 -2.01 -3.42 -7.26 1.85 3.40 -0.74 -3.11 -5.29
Nucleus -5.43 -5.57 -3.57 9.34 15.36 4.93 3.21 26.44 21.11 17.50 1.74 -0.84 24.20 22.76 28.75
Peroxisomes 2.03 2.11 2.10 0.90 -1.46 -2.04 -4.76 -1.45 0.26 -0.28 0.99 1.88 1.42 0.78 -1.18
Vacuole 2.98 3.39 0.62 -4.85 -7.82 -3.96 -5.07 -10.60 -8.66 -0.68 -2.16 -3.88 -12.69 -11.01 -9.01
Vacuole Periphery 3.25 3.41 2.74 -1.43 -2.05 -2.77 1.70 -3.11 -0.87 -3.53 -1.93 3.64 -2.73 -0.84 -4.15
Standard name
Human Ortholog
Description Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Psy4

Psy4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Psy4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available