Standard name | |
---|---|
Human Ortholog | |
Description | Mitogen-activated serine/threonine protein kinase involved in mating; phosphoactivated by Ste7p; substrates include Ste12p, Far1p, Bni1p, Sst2p; inhibits invasive growth during mating by phosphorylating Tec1p, promoting its; inhibits recruitment of Ste5p, Cdc42p-mediated asymmetry and mating morphogenesis |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 3 | 6 | 4 | 4 | 11 | 3 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 10 | 19 | 9 | 7 | 15 | 9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 3 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 26 | 47 | 36 | 38 | 65 | 75 | 72 | 87 | 102 | 88 | 40 | 54 | 40 | 69 | 67 | 92 | 72 | 98 | 8 | 18 | 50 | 23 | 30 | 19 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 11 | 5 | 8 | 1 | 0 | 3 | 1 | 3 | 3 |
Endosome | 0 | 1 | 2 | 0 | 13 | 21 | 6 | 16 | 4 | 15 | 0 | 0 | 1 | 2 | 1 | 10 | 8 | 2 | 1 | 3 | 7 | 1 | 3 | 5 |
Golgi | 0 | 4 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 1 | 5 | 2 | 4 | 0 | 0 | 1 | 0 | 3 | 9 |
Mitochondria | 4 | 1 | 1 | 1 | 1 | 20 | 73 | 27 | 85 | 64 | 79 | 49 | 1 | 0 | 0 | 3 | 4 | 2 | 1 | 0 | 7 | 1 | 0 | 0 |
Nucleus | 23 | 50 | 59 | 84 | 59 | 81 | 69 | 91 | 97 | 106 | 56 | 67 | 16 | 31 | 26 | 34 | 23 | 18 | 93 | 179 | 114 | 45 | 78 | 66 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 |
Nucleolus | 0 | 1 | 0 | 1 | 1 | 0 | 5 | 1 | 2 | 4 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 2 | 2 | 2 | 3 | 2 | 2 | 3 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 3 | 4 | 2 | 2 | 5 |
Vac/Vac Membrane | 76 | 165 | 121 | 64 | 152 | 138 | 84 | 103 | 88 | 83 | 35 | 53 | 55 | 86 | 71 | 209 | 173 | 136 | 15 | 32 | 34 | 19 | 44 | 55 |
Unique Cell Count | 113 | 220 | 201 | 168 | 261 | 285 | 269 | 299 | 329 | 330 | 180 | 198 | 101 | 156 | 143 | 297 | 244 | 234 | 143 | 264 | 245 | 110 | 189 | 189 |
Labelled Cell Count | 129 | 269 | 219 | 189 | 294 | 340 | 319 | 329 | 386 | 375 | 214 | 229 | 118 | 191 | 166 | 370 | 292 | 272 | 143 | 264 | 245 | 110 | 189 | 189 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.1 | 16.6 | 10.3 | 10.5 | 9.4 | 7.7 | 6.5 | 7.4 | 6.1 | 6.5 | 5.6 | 6.1 | 13.6 | 14.0 | 13.0 | 11.6 | 11.5 | 10.8 | 22.5 | 30.4 | 40.0 |
Std Deviation (1e-4) | 3.0 | 3.8 | 2.0 | 1.9 | 1.6 | 1.9 | 1.5 | 1.6 | 1.4 | 1.5 | 1.3 | 1.2 | 3.2 | 3.0 | 2.6 | 2.1 | 2.2 | 2.0 | 8.1 | 10.8 | 13.9 |
Intensity Change (Log2) | – | – | – | 0.03 | -0.13 | -0.41 | -0.66 | -0.47 | -0.75 | -0.67 | -0.88 | -0.76 | 0.41 | 0.44 | 0.34 | 0.18 | 0.16 | 0.08 | 1.13 | 1.56 | 1.96 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.1 | 1.8 | 2.2 | 2.3 | 2.8 | 3.3 | 2.3 | 1.1 | 2.2 | 4.1 | 5.4 | 5.8 | 3.3 | 2.8 | 5.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 2.4 | 3.3 | 0 | 2.6 | 0 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.5 | 7.8 | 4.1 | 7.7 | 6.4 | 10.4 | 7.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 4.1 | -1.6 | -0.2 | -0.9 | 0.3 | 0 | 0.7 | 0.4 | 1.0 | -2.6 | -2.0 | -2.4 | -5.0 | -5.4 | -5.9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -4.2 | -0.4 | -2.6 | -6.3 | -5.7 | -7.6 | -8.1 | -8.1 | -6.7 | -1.0 | -1.0 | -1.9 | 2.4 | 2.4 | -0.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.7127 | 10.1855 | 8.24 | 8.5165 | 7.4308 | 8.5179 | 7.4669 | 9.154 | 8.1443 | 7.7605 | 6.7706 | 7.6637 | 5.3523 | 7.1821 | 6.934 | 4.9172 | 4.0845 | 6.6238 |
Actin | 0.0095 | 0.0011 | 0.0022 | 0.0028 | 0.0044 | 0.0016 | 0.0025 | 0.0001 | 0.0003 | 0 | 0.0042 | 0 | 0.0686 | 0.0019 | 0.034 | 0.0018 | 0.0172 | 0.0005 |
Bud | 0.0011 | 0.0001 | 0.0012 | 0.0003 | 0.0009 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0009 | 0 | 0.0027 | 0.0004 | 0.0003 | 0.003 | 0.0009 | 0.0002 |
Bud Neck | 0.0047 | 0.0003 | 0.0005 | 0.0015 | 0.0186 | 0.0029 | 0.0011 | 0.0003 | 0.0003 | 0.0002 | 0.0017 | 0.0016 | 0.0027 | 0.0003 | 0.0004 | 0.0072 | 0.0011 | 0.0017 |
Bud Periphery | 0.0023 | 0 | 0.0005 | 0.0004 | 0.0031 | 0.0002 | 0.0006 | 0.0001 | 0.0002 | 0 | 0.0005 | 0 | 0.0027 | 0.0004 | 0.0002 | 0.0041 | 0.0014 | 0.0001 |
Bud Site | 0.0022 | 0.0014 | 0.001 | 0.0006 | 0.0016 | 0.0003 | 0.0007 | 0.0003 | 0.0003 | 0 | 0.0011 | 0 | 0.0228 | 0.0008 | 0.0006 | 0.0009 | 0.0026 | 0.0002 |
Cell Periphery | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0007 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0 |
Cytoplasm | 0.041 | 0.0263 | 0.0297 | 0.0058 | 0.0355 | 0.0226 | 0.0261 | 0.0284 | 0.0106 | 0.0032 | 0.0119 | 0.0095 | 0.0405 | 0.035 | 0.0387 | 0.0423 | 0.0124 | 0.0359 |
Cytoplasmic Foci | 0.0178 | 0.0033 | 0.0076 | 0.0042 | 0.0291 | 0.0075 | 0.0053 | 0.0002 | 0.0009 | 0 | 0.0007 | 0.0001 | 0.0528 | 0.0036 | 0.0092 | 0.004 | 0.0242 | 0.0213 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.0127 | 0.0013 | 0.0019 | 0.0003 | 0.0095 | 0.0018 | 0.0015 | 0.0005 | 0.0005 | 0 | 0.0024 | 0.0002 | 0.0096 | 0.0019 | 0.0034 | 0.0049 | 0.0037 | 0.0009 |
Endosome | 0.031 | 0.0042 | 0.0108 | 0.0167 | 0.0332 | 0.0092 | 0.004 | 0.0002 | 0.0022 | 0 | 0.0157 | 0.0003 | 0.0489 | 0.0086 | 0.0201 | 0.0813 | 0.0842 | 0.0019 |
Golgi | 0.0122 | 0.0005 | 0.0051 | 0.0168 | 0.0029 | 0.0065 | 0.0007 | 0 | 0.0007 | 0 | 0.0017 | 0 | 0.0149 | 0.0075 | 0.0156 | 0.0102 | 0.0362 | 0.0006 |
Lipid Particles | 0.0077 | 0.0016 | 0.0024 | 0.0014 | 0.0096 | 0.0053 | 0.0017 | 0 | 0.0004 | 0 | 0.0019 | 0 | 0.0303 | 0.0005 | 0.0023 | 0.002 | 0.0356 | 0.0026 |
Mitochondria | 0.0165 | 0.0003 | 0.0073 | 0.0157 | 0.0028 | 0.0027 | 0.0042 | 0.0001 | 0.0024 | 0 | 0.0014 | 0.0001 | 0.0184 | 0.0029 | 0.0391 | 0.0129 | 0.1599 | 0.0007 |
None | 0.0111 | 0.0027 | 0.0121 | 0.0001 | 0.0039 | 0.0004 | 0.0007 | 0.0001 | 0.0005 | 0 | 0.0011 | 0.0001 | 0.0401 | 0.033 | 0.0003 | 0.001 | 0.0007 | 0.0023 |
Nuclear Periphery | 0.0404 | 0.0145 | 0.0099 | 0.0041 | 0.0191 | 0.0081 | 0.0108 | 0.0077 | 0.0046 | 0.0021 | 0.0319 | 0.0065 | 0.0339 | 0.0131 | 0.0086 | 0.0107 | 0.0119 | 0.0118 |
Nucleolus | 0.0028 | 0.0015 | 0.0017 | 0.0009 | 0.0037 | 0.0023 | 0.002 | 0.0011 | 0.0012 | 0.0006 | 0.002 | 0.0016 | 0.0081 | 0.0011 | 0.0005 | 0.0014 | 0.0012 | 0.0016 |
Nucleus | 0.7524 | 0.9343 | 0.897 | 0.9215 | 0.7943 | 0.9172 | 0.9253 | 0.9506 | 0.9639 | 0.993 | 0.9128 | 0.972 | 0.5595 | 0.8844 | 0.8224 | 0.7923 | 0.586 | 0.9102 |
Peroxisomes | 0.0072 | 0.0006 | 0.0017 | 0.0008 | 0.0039 | 0.0043 | 0.0067 | 0 | 0.0011 | 0 | 0.0003 | 0 | 0.0129 | 0.0003 | 0.002 | 0.0005 | 0.0063 | 0.0029 |
Punctate Nuclear | 0.0161 | 0.0045 | 0.0048 | 0.0032 | 0.0159 | 0.0027 | 0.0029 | 0.0091 | 0.0088 | 0.0008 | 0.0053 | 0.0075 | 0.008 | 0.0022 | 0.0011 | 0.001 | 0.0084 | 0.0034 |
Vacuole | 0.0086 | 0.0013 | 0.002 | 0.0023 | 0.0063 | 0.0035 | 0.0018 | 0.0009 | 0.0009 | 0 | 0.001 | 0.0004 | 0.0144 | 0.0015 | 0.0005 | 0.0106 | 0.0037 | 0.0009 |
Vacuole Periphery | 0.0024 | 0.0002 | 0.0006 | 0.0005 | 0.0008 | 0.0007 | 0.0009 | 0.0001 | 0.0002 | 0 | 0.0012 | 0.0001 | 0.0069 | 0.0005 | 0.0004 | 0.0078 | 0.002 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.5848 | 8.3591 | 14.4733 | 14.0482 | 21.5535 | 8.3073 | 14.7908 | 15.2119 | 11.9107 | 16.4056 |
Translational Efficiency | 0.943 | 1.4279 | 0.8366 | 0.8677 | 0.9195 | 1.3604 | 0.6301 | 0.762 | 0.9311 | 1.0398 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1741 | 146 | 386 | 1248 | 1543 | 1798 | 287 | 978 | 3284 | 1944 | 673 | 2226 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1069.20 | 1337.42 | 1512.88 | 1317.87 | 1088.24 | 1365.59 | 1386.91 | 1291.57 | 1078.15 | 1363.47 | 1459.16 | 1306.32 |
Standard Deviation | 166.22 | 307.58 | 247.18 | 304.74 | 168.05 | 270.72 | 217.14 | 216.32 | 167.35 | 273.76 | 242.96 | 269.81 |
Intensity Change Log 2 | — | 0.322921 | 0.500766 | 0.301676 | — | 0.327528 | 0.349877 | 0.247129 | — | 0.325246 | 0.426628 | 0.274420 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000340 | 0.001082 | 0.001317 | 0.001060 | 0.000736 | 0.000707 | 0.001547 | 0.001438 | 0.000526 | 0.000735 | 0.001415 | 0.001226 |
Bud Neck | 0.004556 | 0.007901 | 0.016915 | 0.014509 | 0.005708 | 0.015530 | 0.008046 | 0.021626 | 0.005097 | 0.014957 | 0.013133 | 0.017636 |
Bud Site | 0.001475 | 0.003818 | 0.005463 | 0.008770 | 0.002339 | 0.006123 | 0.004547 | 0.032686 | 0.001881 | 0.005950 | 0.005072 | 0.019277 |
Cell Periphery | 0.000129 | 0.000304 | 0.000355 | 0.000388 | 0.000235 | 0.000243 | 0.000326 | 0.000345 | 0.000179 | 0.000247 | 0.000343 | 0.000369 |
Cytoplasm | 0.116823* | 0.119945* | 0.042918 | 0.176725* | 0.095980 | 0.075555 | 0.049773 | 0.099470 | 0.107030* | 0.078889 | 0.045842 | 0.142783* |
Cytoplasmic Foci | 0.001291 | 0.001895 | 0.000600 | 0.001775 | 0.001680 | 0.002159 | 0.002390 | 0.035606 | 0.001474 | 0.002139 | 0.001363 | 0.016638 |
Eisosomes | 0.000012 | 0.000022 | 0.000038 | 0.000022 | 0.000017 | 0.000019 | 0.000081 | 0.000030 | 0.000014 | 0.000019 | 0.000056 | 0.000025 |
Endoplasmic Reticulum | 0.004343 | 0.004902 | 0.004182 | 0.003659 | 0.006149 | 0.004018 | 0.010687 | 0.001891 | 0.005191 | 0.004084 | 0.006956 | 0.002882 |
Endosome | 0.000277 | 0.000325 | 0.000566 | 0.001372 | 0.000607 | 0.001321 | 0.001303 | 0.044157 | 0.000432 | 0.001246 | 0.000880 | 0.020170 |
Golgi | 0.000073 | 0.000142 | 0.000139 | 0.007719 | 0.000168 | 0.001176 | 0.000126 | 0.027484 | 0.000118 | 0.001098 | 0.000133 | 0.016403 |
Lipid Particles | 0.000180 | 0.000256 | 0.000462 | 0.000421 | 0.000373 | 0.000510 | 0.001187 | 0.001180 | 0.000271 | 0.000491 | 0.000771 | 0.000754 |
Mitochondria | 0.000534 | 0.000817 | 0.002252 | 0.004210 | 0.001088 | 0.001658 | 0.001394 | 0.016046 | 0.000794 | 0.001595 | 0.001886 | 0.009410 |
Mitotic Spindle | 0.000279 | 0.004164 | 0.010539 | 0.031396 | 0.003276 | 0.016271 | 0.001449 | 0.325913* | 0.001687 | 0.015362 | 0.006663 | 0.160793* |
None | 0.004798 | 0.005145 | 0.006709 | 0.014888 | 0.002890 | 0.006157 | 0.004711 | 0.018976 | 0.003902 | 0.006081 | 0.005857 | 0.016684 |
Nuclear Periphery | 0.000812 | 0.000429 | 0.001406 | 0.001604 | 0.001371 | 0.001178 | 0.001265 | 0.001378 | 0.001075 | 0.001121 | 0.001346 | 0.001505 |
Nuclear Periphery Foci | 0.000659 | 0.001603 | 0.000571 | 0.002983 | 0.000862 | 0.001854 | 0.001362 | 0.001400 | 0.000754 | 0.001835 | 0.000908 | 0.002288 |
Nucleolus | 0.000355 | 0.000453 | 0.000638 | 0.000542 | 0.000491 | 0.000948 | 0.000850 | 0.003361 | 0.000419 | 0.000911 | 0.000728 | 0.001781 |
Nucleus | 0.857180* | 0.838064* | 0.888771* | 0.708322* | 0.868832* | 0.856771* | 0.873969* | 0.321548* | 0.862655* | 0.855366* | 0.882459* | 0.538392* |
Peroxisomes | 0.000052 | 0.000093 | 0.000113 | 0.000080 | 0.000192 | 0.000344 | 0.000235 | 0.009222 | 0.000118 | 0.000325 | 0.000165 | 0.004096 |
Vacuole | 0.005745 | 0.008549 | 0.015931 | 0.015845 | 0.006877 | 0.007137 | 0.034615 | 0.030731 | 0.006277 | 0.007243 | 0.023899 | 0.022385 |
Vacuole Periphery | 0.000086 | 0.000089 | 0.000116 | 0.003710 | 0.000130 | 0.000324 | 0.000137 | 0.005511 | 0.000107 | 0.000307 | 0.000125 | 0.004501 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.06 | -11.47 | -14.83 | -0.48 | 3.22 | -0.16 | -3.90 | -2.50 | -6.92 | 3.33 | -1.62 | -7.96 | -5.75 | -7.81 | 4.57 |
Bud Neck | -2.63 | -7.26 | -13.17 | -6.45 | -0.51 | -10.15 | -3.76 | -12.33 | -5.56 | -6.57 | -11.29 | -8.09 | -17.98 | -6.48 | -4.05 |
Bud Site | -3.21 | -7.91 | -9.73 | -4.11 | -1.45 | -4.12 | -4.60 | -10.28 | -8.42 | -7.60 | -5.47 | -8.90 | -13.51 | -8.70 | -6.98 |
Cell Periphery | -3.10 | -10.45 | -11.97 | -0.37 | 1.93 | -0.04 | -3.12 | -1.57 | -2.25 | 2.28 | -2.79 | -9.16 | -7.97 | -4.62 | 2.92 |
Cytoplasm | 0.24 | 13.93 | 4.69 | 1.38 | -8.36 | 5.10 | 9.52 | 4.14 | -0.37 | -5.77 | 7.51 | 16.74 | 6.12 | -1.20 | -10.15 |
Cytoplasmic Foci | -0.78 | 1.65 | -0.63 | 0.58 | -1.95 | 0.76 | 0.82 | -3.32 | -3.85 | -3.86 | -0.14 | 1.62 | -3.21 | -2.90 | -4.32 |
Eisosomes | -3.80 | -12.86 | -15.66 | -3.11 | 4.13 | -2.13 | -11.64 | -13.75 | -13.27 | 4.98 | -6.02 | -16.94 | -20.47 | -15.41 | 6.36 |
Endoplasmic Reticulum | -1.22 | -2.36 | -1.04 | 0.73 | 1.43 | 5.06 | -6.57 | 4.71 | -0.35 | 9.25 | 3.66 | -6.33 | 2.54 | -1.27 | 7.97 |
Endosome | -1.61 | -5.75 | -5.55 | -3.09 | -0.24 | -1.82 | -4.03 | -4.42 | -3.95 | -3.58 | -2.84 | -6.30 | -5.08 | -3.55 | -3.57 |
Golgi | -2.91 | -6.04 | -1.95 | -1.85 | -1.77 | -2.18 | -0.37 | -3.41 | -2.30 | -3.39 | -2.40 | -2.57 | -3.52 | -2.11 | -3.37 |
Lipid Particles | -2.06 | -6.73 | -9.66 | -5.32 | -1.22 | -0.60 | -6.45 | -11.42 | -6.56 | -3.21 | -1.37 | -9.11 | -14.62 | -5.52 | -3.01 |
Mitochondria | -4.35 | -20.10 | -9.73 | -8.06 | -2.58 | -1.30 | -1.66 | -3.45 | -3.16 | -2.98 | -3.24 | -6.48 | -6.81 | -5.33 | -3.80 |
Mitotic Spindle | -1.09 | -3.04 | -7.97 | -3.48 | -2.35 | -4.59 | -0.22 | -16.32 | -14.79 | -16.30 | -5.75 | -2.94 | -17.41 | -13.63 | -13.54 |
None | -0.27 | -0.61 | -5.72 | -4.40 | -4.42 | -4.05 | -1.23 | -10.40 | -7.38 | -9.12 | -2.70 | -1.21 | -11.40 | -7.75 | -9.22 |
Nuclear Periphery | 3.06 | -9.54 | -15.20 | -15.37 | -5.04 | -0.58 | -3.17 | -16.61 | -16.03 | -13.04 | -3.28 | -9.25 | -22.10 | -17.55 | -12.09 |
Nuclear Periphery Foci | -1.89 | -0.30 | -3.79 | -1.81 | -3.67 | -0.76 | -1.38 | -3.49 | -2.04 | -2.72 | -1.24 | -1.15 | -5.08 | -2.39 | -4.59 |
Nucleolus | -1.20 | -4.65 | -3.86 | -3.66 | -2.78 | -2.09 | -3.43 | -7.77 | -6.05 | -7.08 | -2.38 | -5.64 | -8.05 | -5.24 | -6.77 |
Nucleus | 1.04 | 0.10 | 9.92 | 3.13 | 6.88 | 1.33 | 0.78 | 24.59 | 23.76 | 19.00 | 1.15 | 0.60 | 23.87 | 20.86 | 17.74 |
Peroxisomes | -2.42 | -7.00 | -5.79 | -1.02 | 1.25 | -0.65 | -0.42 | -2.84 | -2.71 | -2.80 | -1.66 | -2.23 | -3.01 | -2.38 | -2.74 |
Vacuole | -1.77 | -6.32 | -12.48 | -7.32 | -0.60 | -2.17 | -6.50 | -16.10 | -15.50 | -5.02 | -3.11 | -9.04 | -19.92 | -18.07 | -4.26 |
Vacuole Periphery | -0.61 | -3.42 | -1.73 | -1.70 | -1.56 | -1.99 | -1.07 | -2.76 | -1.99 | -2.60 | -2.55 | -2.85 | -2.49 | -1.92 | -2.28 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|