Standard name
Human Ortholog
Description Subunit of the HIR complex; HIR is a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.05 0 0.05 0 0 0 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.05 – 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.1 0.16 0.15 0.19 0.17 0.16 0.18 0.07 – 0.06 0.08 0.15 0.19 0.26 0.12 0.11 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0.05 0.06 0.06 0.1 0.05 0 0 0 0.05 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0.1 0.13 0.13 0.14 0.3 0.42 0.28 0.67 – 0.71 0.51 0.07 0 0 0.07 0.05 0.17 0 0 0.06 0 0.05 0
Nucleus 0.81 0.76 0.64 0.68 0.58 0.53 0.52 0.5 0.47 – 0.51 0.54 0.76 0.68 0.59 0.74 0.68 0.7 0.58 0.54 0.44 0.31 0.33 0.34
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.12 0.13 0.1 0.13 0.12 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0.05 0.19 0.2 0.12 0.15 0.08 0.13 0.08 – 0.06 0.12 0 0 0 0.06 0.09 0.1 0.08 0.06 0.08 0.1 0.14 0.1
Vac/Vac Membrane 0.05 0.06 0 0 0 0 0 0 0 – 0 0 0 0.05 0.07 0.05 0.08 0.06 0.08 0.13 0.17 0.2 0.2 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 1 3 3
Bud 1 0 2 4 1 1 15 7 12 – 11 12 0 0 1 1 0 0 5 6 3 7 8 18
Bud Neck 2 2 15 5 15 6 20 15 19 – 7 6 0 0 0 1 0 0 2 3 4 1 8 13
Bud Site 2 3 4 3 4 10 11 8 22 – 22 17 2 1 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 8 45 47 32 56 64 83 92 29 – 27 36 47 62 73 34 32 19 4 12 14 4 9 16
Endoplasmic Reticulum 1 2 1 0 0 1 0 1 0 – 0 1 0 2 1 7 20 10 0 0 1 4 5 4
Endosome 5 7 6 7 4 6 3 7 3 – 1 6 7 16 16 16 29 8 10 21 15 11 7 25
Golgi 0 2 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 2 2 0 2 1
Mitochondria 28 45 39 27 40 113 217 143 275 – 299 236 21 10 8 21 16 28 4 15 25 8 18 16
Nucleus 179 342 189 142 170 202 267 252 191 – 216 249 232 221 166 209 208 112 152 298 196 65 122 161
Nuclear Periphery 1 0 0 0 1 0 0 3 0 – 0 3 0 1 0 1 1 0 2 5 4 3 3 8
Nucleolus 2 0 1 1 1 2 4 4 4 – 3 4 7 1 7 0 5 2 32 71 46 27 45 47
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 2 2 0 0 0 0 0 0 0 0 0
SpindlePole 7 23 56 41 34 56 40 64 34 – 24 54 7 12 5 17 26 16 20 33 35 21 51 46
Vac/Vac Membrane 11 29 2 1 13 14 11 16 4 – 7 13 11 16 20 14 23 10 20 69 74 41 74 94
Unique Cell Count 220 451 297 210 292 379 512 502 408 422 460 306 327 282 284 304 161 260 553 448 209 374 480
Labelled Cell Count 248 500 362 263 339 475 671 612 593 617 637 334 344 300 321 360 205 260 553 448 209 374 480


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 4.7 4.9 4.5 5.0 4.1 4.2 4.6 3.6 – 3.5 4.0 4.8 4.9 4.7 6.4 6.7 6.2 4.5 4.3 4.7
Std Deviation (1e-4) 0.5 0.8 1.2 1.2 1.3 1.2 1.0 1.1 0.9 – 0.9 0.9 1.5 1.6 1.3 1.1 1.4 1.3 1.5 1.7 2.0
Intensity Change (Log2) – – – -0.11 0.03 -0.26 -0.23 -0.09 -0.45 – -0.47 -0.29 -0.03 0.0 -0.06 0.37 0.44 0.33 -0.12 -0.18 -0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 –
Bud Neck – – – – – – – – – – – – 0 0 –
Bud Site – – – – – – – – – – – – 0 0 –
Cell Periphery – – – – – – – – – – – – 0 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 –
Endosome – – – – – – – – – – – – 0 0 –
Golgi – – – – – – – – – – – – 0 0 –
Mitochondria – – – – – – – – – – – – 0 0 –
Nuclear Periphery – – – – – – – – – – – – 0 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 –
Peroxisomes – – – – – – – – – – – – 0 0 –
SpindlePole – – – – – – – – – – – – 0 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 –
Cytoplasm – – – – – – – – – – – – 0 0 –
Nucleus – – – – – – – – – – – – 0 0 –
Vacuole – – – – – – – – – – – – 3.1 4.2 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4463 1.7454 1.4518 1.2549 0.8421 1.4902 1.1962 0.1918 0.6096 0.4441 0.9088 0.5052 -0.3515 -0.4683 -0.5716 -0.5026 -0.6231 -0.4017
Actin 0.0095 0.012 0.0009 0.005 0.0112 0.0023 0.0398 0.0019 0.0103 0.0115 0.0855 0.0063 0.0082 0.0003 0.0004 0.0014 0.001 0.0002
Bud 0.0003 0.0006 0.0003 0.0016 0.0005 0.0002 0.0021 0.0008 0.0009 0.0002 0.0005 0.0004 0.0001 0.0004 0.0001 0 0.0002 0.0001
Bud Neck 0.0014 0.0015 0.0005 0.004 0.002 0.0087 0.0179 0.0015 0.003 0.001 0.0017 0.0036 0.0028 0.0006 0.0008 0.0107 0.0037 0.0038
Bud Periphery 0.0004 0.0008 0.0017 0.0016 0.0016 0.0002 0.0048 0.0011 0.0013 0.0005 0.0007 0.0007 0.0003 0.0001 0.0001 0 0.0002 0.0002
Bud Site 0.0018 0.0104 0.001 0.0024 0.0009 0.0004 0.0146 0.0058 0.02 0.0004 0.0018 0.0009 0.0004 0.0011 0.0003 0.0001 0.0005 0.0002
Cell Periphery 0.0003 0.0002 0.0001 0.0003 0.0002 0 0.0011 0.0001 0.0004 0.0001 0.0001 0.0001 0.0001 0 0 0 0 0
Cytoplasm 0.0302 0.0126 0.0207 0.0169 0.0084 0.0083 0.0227 0.0557 0.0187 0.022 0.0149 0.008 0.0268 0.0771 0.051 0.0456 0.0633 0.0228
Cytoplasmic Foci 0.0183 0.0014 0.003 0.0136 0.0162 0.0009 0.0239 0.0026 0.0097 0.0161 0.0102 0.0023 0.0048 0.0059 0.0036 0.0011 0.0053 0.0011
Eisosomes 0.0013 0.0003 0.0002 0.0028 0.0002 0.0001 0.0008 0 0.0001 0.0001 0.0006 0 0.0001 0.0001 0 0.0001 0.0002 0.0001
Endoplasmic Reticulum 0.0036 0.0019 0.0014 0.0015 0.0026 0.0007 0.0054 0.0011 0.0046 0.0025 0.0009 0.001 0.0015 0.0015 0.001 0.0014 0.0012 0.0005
Endosome 0.0153 0.0013 0.0038 0.0346 0.0302 0.002 0.0278 0.0023 0.0112 0.0318 0.0152 0.0059 0.0019 0.0028 0.0026 0.0008 0.0031 0.0008
Golgi 0.0031 0.0002 0.0005 0.0062 0.0113 0.0017 0.0093 0.0004 0.004 0.0109 0.0194 0.0022 0.0008 0.0001 0.0001 0.0001 0.0002 0
Lipid Particles 0.0083 0.0005 0.0026 0.0158 0.0153 0.0012 0.0236 0.0013 0.0101 0.0182 0.0172 0.0019 0.0064 0.0005 0.0006 0.0001 0.0002 0.0001
Mitochondria 0.0052 0.0018 0.0107 0.0039 0.0308 0.0044 0.0225 0.0005 0.0019 0.0032 0.0096 0.0014 0.0005 0.0009 0.0007 0.0005 0.0009 0.0003
None 0.1162 0.0997 0.1043 0.057 0.0084 0.0469 0.0482 0.1039 0.0836 0.0634 0.0174 0.0061 0.141 0.289 0.2124 0.3381 0.1901 0.1232
Nuclear Periphery 0.0205 0.0214 0.0083 0.0102 0.0286 0.0051 0.0244 0.0128 0.0196 0.0357 0.0144 0.0091 0.006 0.0167 0.0223 0.0089 0.0157 0.0046
Nucleolus 0.0188 0.0285 0.018 0.024 0.0387 0.0362 0.0276 0.025 0.0188 0.0227 0.0219 0.0404 0.0107 0.0081 0.0125 0.0044 0.0219 0.0217
Nucleus 0.6541 0.7442 0.7811 0.6633 0.6709 0.8263 0.6003 0.701 0.696 0.6718 0.6253 0.8485 0.7178 0.5281 0.635 0.5192 0.597 0.7224
Peroxisomes 0.0112 0.0007 0.0013 0.0107 0.012 0.0008 0.0185 0.0011 0.0123 0.0057 0.0086 0.0008 0.0104 0.0003 0.0003 0.0001 0.0004 0.0001
Punctate Nuclear 0.0768 0.0586 0.0381 0.1176 0.1056 0.0529 0.0509 0.0798 0.0706 0.0793 0.133 0.0593 0.0587 0.0645 0.0549 0.0668 0.0931 0.0971
Vacuole 0.0025 0.001 0.0008 0.0059 0.0023 0.0004 0.0107 0.001 0.0021 0.0018 0.0005 0.0009 0.0006 0.0015 0.0008 0.0004 0.0014 0.0006
Vacuole Periphery 0.0009 0.0005 0.0006 0.0012 0.0019 0.0002 0.0032 0.0002 0.0007 0.001 0.0005 0.0003 0.0002 0.0004 0.0004 0.0002 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.0882 16.0291 10.1537 12.7106 10.49 16.6097 13.0447 11.739 11.3501 16.6245
Translational Efficiency 0.7586 0.4591 0.702 0.5847 0.7774 0.6451 0.6331 0.6177 0.677 0.5802

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1320 1953 146 1548 2008 2108 1486 828 3328 4061 1632 2376

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 609.91 745.11 924.75 831.93 672.32 678.35 867.81 1012.64 647.57 710.46 872.90 894.90
Standard Deviation 84.55 115.80 120.79 121.24 79.72 106.59 123.33 134.46 87.19 116.01 124.17 152.62
Intensity Change Log 2 — 0.288857 0.600467 0.447866 — 0.012882 0.368231 0.590901 — 0.150747 0.483366 0.524629

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000109 0.000372 0.002035 0.000963 0.000052 0.000326 0.000799 0.000241 0.000075 0.000348 0.000910 0.000711
Bud Neck 0.003393 0.009781 0.004559 0.036714 0.004491 0.013589 0.017368 0.055003 0.004055 0.011758 0.016222 0.043087
Bud Site 0.000809 0.002448 0.001914 0.020994 0.000673 0.006274 0.006320 0.015336 0.000727 0.004434 0.005926 0.019022
Cell Periphery 0.000156 0.000127 0.000158 0.000342 0.000120 0.000239 0.000147 0.000223 0.000134 0.000185 0.000148 0.000300
Cytoplasm 0.200246* 0.056970 0.010308 0.047880 0.169176* 0.140330* 0.022184 0.127189* 0.181499* 0.100241* 0.021121 0.075518
Cytoplasmic Foci 0.037158 0.008533 0.002741 0.003902 0.023766 0.033501 0.000278 0.000277 0.029078 0.021493 0.000499 0.002639
Eisosomes 0.000051 0.000037 0.000123 0.000028 0.000030 0.000054 0.000026 0.000017 0.000038 0.000046 0.000035 0.000024
Endoplasmic Reticulum 0.000971 0.003201 0.013349 0.007417 0.000878 0.002892 0.005055 0.001720 0.000915 0.003041 0.005797 0.005432
Endosome 0.002038 0.001198 0.001281 0.001996 0.000937 0.003906 0.000608 0.000580 0.001374 0.002604 0.000668 0.001502
Golgi 0.000428 0.000401 0.000215 0.002311 0.000199 0.001613 0.000112 0.000071 0.000290 0.001030 0.000121 0.001531
Lipid Particles 0.006973 0.002459 0.002359 0.000599 0.003311 0.008709 0.000282 0.000113 0.004764 0.005703 0.000468 0.000430
Mitochondria 0.005497 0.004140 0.001103 0.006005 0.002443 0.016140 0.002085 0.005167 0.003655 0.010369 0.001997 0.005713
Mitotic Spindle 0.003328 0.009885 0.002788 0.047620 0.004657 0.016236 0.016246 0.016506 0.004130 0.013182 0.015042 0.036777
None 0.031768 0.011569 0.008321 0.009299 0.012572 0.019188 0.009437 0.012464 0.020186 0.015524 0.009337 0.010402
Nuclear Periphery 0.002662 0.003420 0.001327 0.005061 0.002934 0.006375 0.001836 0.001612 0.002826 0.004954 0.001790 0.003859
Nuclear Periphery Foci 0.001174 0.001716 0.001302 0.001016 0.000575 0.002450 0.000639 0.000078 0.000813 0.002097 0.000698 0.000689
Nucleolus 0.015544 0.026254 0.015872 0.007112 0.009675 0.023805 0.008743 0.003989 0.012003 0.024983 0.009381 0.006024
Nucleus 0.681643* 0.853052* 0.915751* 0.782250* 0.760032* 0.691023* 0.902279* 0.752221* 0.728941* 0.768945* 0.903484* 0.771786*
Peroxisomes 0.003067 0.001813 0.001009 0.000293 0.001054 0.005455 0.000164 0.000072 0.001853 0.003704 0.000240 0.000216
Vacuole 0.002761 0.002272 0.013176 0.013789 0.002226 0.006164 0.005200 0.006748 0.002438 0.004292 0.005914 0.011336
Vacuole Periphery 0.000222 0.000352 0.000309 0.004409 0.000199 0.001732 0.000191 0.000372 0.000208 0.001068 0.000201 0.003002

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.23 -11.64 -17.96 -12.13 7.00 -6.77 -16.09 -13.62 1.05 12.84 -10.94 -18.27 -20.12 -11.33 5.87
Bud Neck -9.40 -4.83 -18.14 -14.27 -13.30 -9.00 -15.12 -11.82 -9.28 -5.79 -12.37 -16.28 -20.99 -16.39 -8.63
Bud Site -2.97 -5.65 -8.26 -6.67 -5.65 -6.86 -6.38 -6.82 -2.86 -2.64 -7.35 -6.67 -10.55 -6.69 -4.70
Cell Periphery 2.04 -2.23 -2.39 -3.31 -0.84 -5.49 -0.99 -1.62 3.55 -1.04 -3.85 -0.64 -3.15 -0.84 -2.90
Cytoplasm 20.08 27.26 22.73 4.37 -9.32 4.52 29.84 9.17 5.61 -13.97 16.97 39.83 25.27 10.43 -15.59
Cytoplasmic Foci 11.95 15.08 15.19 7.16 0.18 -3.79 15.07 15.03 18.03 -0.68 5.12 21.84 20.61 18.37 -2.36
Eisosomes 5.58 -7.58 5.61 -0.22 9.17 -10.80 -4.78 9.52 19.27 12.98 -3.55 -3.68 5.29 9.09 7.76
Endoplasmic Reticulum -12.72 -10.61 -23.24 -15.78 4.85 -11.07 -21.21 -5.79 3.85 16.33 -17.02 -23.06 -23.20 -13.94 3.82
Endosome 2.26 0.47 1.72 -1.12 1.22 -8.24 1.45 4.63 10.13 5.13 -4.69 3.19 1.76 7.26 -2.05
Golgi 0.19 1.21 -1.03 -1.11 -1.40 -4.04 0.55 2.47 4.44 9.34 -3.89 2.23 -1.14 1.30 -1.59
Lipid Particles 6.31 6.47 9.42 8.94 5.98 -7.32 11.56 12.76 12.83 8.05 -1.59 13.50 13.80 14.51 1.65
Mitochondria 0.73 2.54 -0.08 -1.84 -9.25 -10.66 -0.71 -2.94 8.36 -2.87 -7.27 1.59 -2.19 6.74 -7.11
Mitotic Spindle -3.71 -1.01 -10.93 -8.46 -7.42 -5.17 -6.10 -4.18 -0.85 0.72 -6.21 -6.50 -11.55 -7.89 -4.84
None 7.52 7.09 9.24 3.37 -0.89 -4.36 4.53 1.33 6.27 -4.18 3.20 9.82 8.93 7.82 -2.65
Nuclear Periphery -2.18 1.74 -10.17 -8.26 -11.20 -5.33 -1.50 1.03 6.02 2.92 -5.63 -1.78 -9.11 -2.93 -8.15
Nuclear Periphery Foci 0.10 -0.61 1.15 1.64 1.13 -6.03 -0.96 5.81 8.18 4.07 -3.91 0.97 0.61 5.23 -0.54
Nucleolus -4.01 0.83 3.91 8.61 1.57 -10.08 0.18 2.38 10.71 2.10 -10.35 3.26 2.91 12.49 -0.32
Nucleus -17.53 -14.91 -9.68 7.90 7.89 7.94 -16.20 -2.71 -9.14 9.54 -5.64 -23.88 -7.66 -2.69 14.68
Peroxisomes 1.57 3.12 4.35 7.08 7.71 -8.37 5.60 6.60 11.19 7.10 -4.74 5.88 6.29 12.95 4.18
Vacuole 0.66 -5.40 -11.00 -11.11 0.64 -6.79 -7.53 -4.52 -1.19 2.11 -5.15 -8.69 -12.00 -10.24 -3.07
Vacuole Periphery -2.71 -4.47 -2.62 -2.39 -2.30 -5.38 0.27 -1.92 5.10 -2.23 -5.75 -0.17 -2.70 -0.46 -2.69
Standard name
Human Ortholog
Description Subunit of the HIR complex; HIR is a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores
Localization
Cell Percentages nucleus (84%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Hir1

Hir1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hir1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available