Standard name
Human Ortholog
Description Histone H2A; core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.33 0.26 0 0.1 0.06 0.17 – 0.11 0.14 0.14 0.16 0.1 0.09 0 0.1 0.25 0.22 0.33 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0.09 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.18 0.16 0.36 0.29 0.1 0.21 – 0.25 0.32 0.31 0.28 0.33 0.26 0.14 0.2 0.12 0.12 0.08 0 0 0 0 0 0
Nucleus 0.85 0.89 0.81 0.88 0.9 0.85 – 0.76 0.73 0.77 0.74 0.74 0.86 0.84 0.7 0.75 0.72 0.74 0.84 0.84 0.66 0.75 0.63 0.51
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Nucleolus 0.14 0.16 0.49 0.29 0.12 0.2 – 0.32 0.34 0.34 0.37 0.4 0.26 0.31 0.34 0.16 0.18 0.16 0.06 0.06 0.18 0.14 0.23 0.27
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Neck 0 3 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 133 81 3 14 13 42 – 43 34 44 45 35 13 6 14 37 42 64 0 0 1 0 0 2
Endoplasmic Reticulum 0 0 1 0 0 0 – 0 0 0 0 0 0 0 0 10 18 17 1 0 0 0 1 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 72 51 34 41 21 54 – 102 76 101 79 113 39 21 28 17 22 15 0 0 0 0 0 1
Nucleus 346 279 76 126 189 215 – 304 173 249 211 252 128 128 98 110 138 145 319 249 59 51 86 61
Nuclear Periphery 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 15 13 2 0 4 0
Nucleolus 55 49 46 41 25 52 – 127 81 110 106 138 39 47 48 23 34 31 21 18 15 9 30 32
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 2 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 0 2 2 – 3 0 3 2 2 2 2 4 1 0 0 2 2 0 2 4 11
Unique Cell Count 406 314 94 143 210 254 402 238 324 285 341 148 152 141 147 191 196 382 298 90 68 136 121
Labelled Cell Count 607 467 160 222 252 365 579 364 507 443 540 221 205 192 198 255 272 382 298 90 68 136 121


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 172.6 175.0 90.9 128.4 136.6 136.5 – 136.4 140.1 136.8 131.6 125.9 136.0 126.7 104.9 175.4 165.9 172.3 121.6 141.1 129.0
Std Deviation (1e-4) 52.4 56.4 40.2 47.2 53.7 52.9 – 47.1 49.7 47.5 54.2 41.7 40.8 45.8 51.3 64.9 67.7 72.3 55.1 55.6 52.6
Intensity Change (Log2) – – – 0.5 0.59 0.59 – 0.59 0.62 0.59 0.53 0.47 0.58 0.48 0.21 0.95 0.87 0.92 0.42 0.63 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.9 0 3.3 – 2.3 2.9 2.8 3.2 2.2 1.7 0 2.0 4.5 4.1 5.6
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 2.7 2.5
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.2 -5.5 -2.8 – -2.1 -0.7 -0.9 -1.6 -0.6 -1.6 -4.1 -2.8 -4.6 -4.9 -6.1
Nucleus 1.5 2.2 0.8 – -1.1 -1.5 -0.8 -1.3 -1.4 1.2 0.7 -1.9 -1.1 -1.6 -1.3
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -3.2 -7.1 -5.2 – -3.2 -2.5 -2.6 -2.0 -1.5 -3.6 -2.8 -2.3 -5.6 -5.5 -6.0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 134.1042 140.9993 138.5179 134.403 163.7782 134.8522 150.2898 130.0917 140.254 137.6932 124.8847 132.8823 168.2163 145.8916 155.939 154.1605 165.3254 140.3615
Actin 0.0103 0 0 0.0003 0 0 0.0051 0 0.0014 0 0.0005 0.0008 0.0188 0 0.019 0.0479 0.0533 0.0059
Bud 0.0003 0 0 0.0001 0 0.0003 0.0004 0 0.0001 0 0.0001 0.0001 0.0002 0 0.0002 0.0003 0.0003 0.0001
Bud Neck 0.0001 0 0 0.0001 0 0.0001 0.0001 0 0 0 0.0001 0.0001 0.0005 0 0.0022 0.0005 0.0004 0.0002
Bud Periphery 0.0002 0 0 0.0002 0 0.0001 0.0003 0 0.0001 0 0.0001 0.0001 0.0001 0 0.0002 0.0004 0.0007 0.0001
Bud Site 0.0007 0 0 0.0004 0 0 0.0007 0 0.0003 0 0.0001 0.0001 0.0007 0 0.0012 0.0022 0.0003 0.0001
Cell Periphery 0.0011 0 0 0.0001 0.0002 0.0002 0.0012 0 0.0001 0 0.0001 0.0003 0.0005 0 0.0002 0.0003 0.0004 0.0001
Cytoplasm 0.001 0 0.0001 0 0 0.0007 0.0013 0 0.0011 0 0.0002 0.0005 0.0029 0 0.0033 0.0006 0.0001 0.0002
Cytoplasmic Foci 0.0177 0 0.0002 0.0001 0 0.0007 0.0078 0 0.0074 0 0 0.0029 0.0062 0 0.0053 0.003 0.0004 0.0031
Eisosomes 0.0011 0 0 0.0001 0 0 0.0004 0 0.0001 0 0 0.0001 0.0008 0 0.0003 0.0004 0.0003 0.0002
Endoplasmic Reticulum 0.0003 0 0 0 0 0 0.0002 0 0.0004 0 0.0001 0.0002 0.0019 0 0.0006 0.0008 0.0009 0.0004
Endosome 0.0028 0 0 0 0 0 0.0022 0 0.0039 0 0.0002 0.0017 0.007 0 0.0011 0.0056 0.0023 0.0023
Golgi 0.0011 0 0 0 0 0 0.0017 0 0.0006 0 0 0.0005 0.0025 0 0.0007 0.0035 0.0034 0.0006
Lipid Particles 0.0021 0 0 0.0001 0 0.0001 0.0049 0 0.001 0 0 0.0008 0.0034 0 0.0016 0.0054 0.0008 0.0006
Mitochondria 0.0192 0.0011 0.0017 0.0138 0.0254 0.0009 0.0104 0.0004 0.0049 0.0005 0.0051 0.0023 0.0035 0.0005 0.0016 0.005 0.0185 0.0019
None 0.0037 0 0.0002 0.0001 0 0.0001 0.0031 0 0.0008 0 0.0001 0.0013 0.0056 0 0.002 0.0018 0.0002 0.0004
Nuclear Periphery 0.0006 0 0.0017 0.0006 0 0.0001 0.0146 0.0001 0.0063 0 0.0049 0.0052 0.0056 0 0.002 0.0027 0.001 0.0034
Nucleolus 0.1957 0.1009 0.107 0.1608 0.6488 0.235 0.1966 0.0814 0.0593 0.074 0.5643 0.2266 0.1557 0.0902 0.0512 0.0818 0.5972 0.2062
Nucleus 0.7343 0.8978 0.8884 0.8204 0.3247 0.7608 0.7123 0.9173 0.903 0.9253 0.4228 0.7536 0.7749 0.9091 0.894 0.8309 0.3172 0.7728
Peroxisomes 0.0028 0 0 0.0002 0 0 0.004 0 0.0006 0 0 0.0001 0.0008 0 0.0096 0.0035 0.0002 0.0002
Punctate Nuclear 0.0022 0 0.0003 0.0021 0 0.0002 0.0303 0.0006 0.0068 0 0.001 0.0012 0.0074 0 0.0034 0.0023 0.0002 0.0007
Vacuole 0.0011 0 0.0001 0.0001 0.0001 0.0005 0.0013 0 0.0008 0 0.0001 0.0006 0.0006 0 0.0002 0.0004 0.0004 0.0002
Vacuole Periphery 0.0014 0.0001 0.0002 0.0003 0.0005 0.0003 0.0013 0 0.0009 0 0.0003 0.0009 0.0006 0.0001 0.0002 0.0007 0.0014 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 660.9365 904.7962 207.2895 157.144 772.674 458.8064 877.3451 630.1071 385.2703 685.4248
Translational Efficiency 2.0028 1.959 1.5308 1.8476 1.8676 2.5578 1.7166 1.6384 1.4844 2.1381

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1720 876 1222 803 986 1932 2554 134 2706 2808 3776 937

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 6550.97 8543.37 10698.32 9098.19 6285.05 8630.90 10443.10 8548.82 6454.08 8603.59 10525.70 9019.62
Standard Deviation 2533.79 2684.78 2295.86 3189.36 2355.33 2542.62 2619.70 3412.59 2473.57 2588.13 2522.29 3227.97
Intensity Change Log 2 — 0.383097 0.707604 0.473871 — 0.457587 0.732554 0.443801 — 0.420051 0.719874 0.459226

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001017 0.002791 0.005376 0.002745 0.000982 0.002050 0.007879 0.002190 0.001005 0.002281 0.007069 0.002666
Bud Neck 0.000977 0.002109 0.002693 0.004359 0.001184 0.001466 0.001961 0.004076 0.001052 0.001667 0.002198 0.004318
Bud Site 0.000204 0.000849 0.000278 0.000620 0.000329 0.000293 0.000174 0.000823 0.000249 0.000467 0.000208 0.000649
Cell Periphery 0.000081 0.000158 0.000079 0.000102 0.000046 0.000052 0.000172 0.000101 0.000068 0.000085 0.000142 0.000102
Cytoplasm 0.000242 0.000225 0.000511 0.000397 0.000046 0.000059 0.000197 0.000532 0.000171 0.000111 0.000298 0.000417
Cytoplasmic Foci 0.001847 0.006296 0.000566 0.003793 0.004710 0.003650 0.000226 0.007826 0.002890 0.004476 0.000336 0.004370
Eisosomes 0.002075 0.004165 0.003215 0.000361 0.000953 0.004440 0.008249 0.000185 0.001666 0.004354 0.006620 0.000336
Endoplasmic Reticulum 0.000097 0.000246 0.000460 0.000362 0.000076 0.000191 0.001722 0.000230 0.000089 0.000208 0.001314 0.000343
Endosome 0.000002 0.000006 0.000002 0.000691 0.000001 0.000004 0.000002 0.000011 0.000002 0.000005 0.000002 0.000594
Golgi 0.000008 0.000022 0.000013 0.000372 0.000016 0.000011 0.000006 0.000088 0.000011 0.000015 0.000008 0.000331
Lipid Particles 0.000002 0.000018 0.000005 0.000096 0.000002 0.000003 0.000004 0.000010 0.000002 0.000008 0.000005 0.000084
Mitochondria 0.001074 0.002156 0.003733 0.005201 0.001343 0.001347 0.001700 0.003618 0.001172 0.001599 0.002358 0.004975
Mitotic Spindle 0.000004 0.000016 0.000005 0.001393 0.000006 0.000138 0.000001 0.001584 0.000005 0.000100 0.000002 0.001420
None 0.001474 0.001309 0.000551 0.001395 0.000913 0.000983 0.000382 0.001869 0.001270 0.001085 0.000437 0.001462
Nuclear Periphery 0.000036 0.000154 0.001401 0.001085 0.000016 0.000077 0.001850 0.000534 0.000029 0.000101 0.001705 0.001006
Nuclear Periphery Foci 0.000004 0.000020 0.000047 0.000276 0.000003 0.000009 0.000019 0.000029 0.000003 0.000013 0.000028 0.000241
Nucleolus 0.091279 0.212627 0.352300* 0.211336 0.147965 0.189371 0.197724 0.132639 0.111934 0.196626 0.247749* 0.200081
Nucleus 0.886812* 0.736753* 0.593185* 0.748522* 0.809120* 0.776937* 0.745289* 0.823213* 0.858503* 0.764401* 0.696065* 0.759203*
Peroxisomes 0.011187 0.018943 0.021436 0.012079 0.031169 0.016051 0.005467 0.016306 0.018468 0.016953 0.010635 0.012683
Vacuole 0.000753 0.004969 0.004238 0.001350 0.000422 0.001306 0.014983 0.001130 0.000633 0.002449 0.011506 0.001318
Vacuole Periphery 0.000824 0.006168 0.009905 0.003466 0.000699 0.001558 0.011993 0.003007 0.000778 0.002996 0.011318 0.003400

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -9.60 -14.49 -8.88 0.35 7.62 -10.51 -22.69 -1.49 -0.10 6.95 -14.33 -26.64 -8.26 -1.61 14.98
Bud Neck -4.95 -13.60 -7.34 -4.78 -4.15 -3.16 -7.39 -4.83 -4.45 -3.73 -7.03 -15.43 -8.09 -6.71 -5.69
Bud Site -2.84 -1.78 -3.15 -0.12 -2.75 0.52 2.49 -1.85 -1.95 -2.27 -2.86 1.13 -3.38 -2.12 -3.56
Cell Periphery -1.00 0.08 -1.01 0.61 -1.17 -0.29 -3.83 -1.41 -1.46 1.62 -0.62 -3.18 -1.71 -0.79 1.24
Cytoplasm 0.08 -0.58 -1.06 -1.13 0.27 -0.74 -0.91 -2.95 -2.76 -1.46 0.68 -0.64 -1.96 -2.58 -0.52
Cytoplasmic Foci -2.34 2.29 -1.82 0.69 -2.68 0.51 4.09 -0.54 -0.66 -1.28 -1.76 4.78 -1.49 -0.34 -3.07
Eisosomes -3.25 -1.90 4.01 7.88 6.81 -8.30 -13.79 5.16 10.80 15.86 -6.47 -10.68 4.79 12.92 16.87
Endoplasmic Reticulum -4.88 -8.73 -4.04 -1.65 1.21 -7.96 -12.43 -1.27 -0.29 8.43 -8.53 -13.43 -4.32 -2.26 8.91
Endosome -2.40 -2.44 -1.50 -1.49 -1.50 -1.32 -3.20 -2.35 -1.55 -2.21 -2.20 -3.24 -1.51 -1.50 -1.51
Golgi -1.64 -3.96 -1.48 -1.42 -1.47 1.77 7.08 -3.96 -4.13 -4.44 -1.42 3.41 -1.53 -1.51 -1.55
Lipid Particles -1.85 -2.40 -1.60 -1.18 -1.55 -2.54 -4.64 -1.69 -1.27 -1.19 -2.10 -2.88 -1.63 -1.46 -1.58
Mitochondria -7.43 -17.35 -3.41 -2.49 -1.17 -0.10 -4.39 -3.01 -3.00 -2.53 -6.55 -16.36 -3.65 -3.23 -2.49
Mitotic Spindle -1.39 -0.21 -2.11 -2.09 -2.10 -2.25 2.64 -1.58 -1.45 -1.58 -2.36 1.26 -2.42 -2.26 -2.43
None 0.38 3.89 0.17 -0.23 -3.39 -0.21 7.48 -1.94 -1.50 -2.98 0.60 5.46 -0.79 -1.15 -4.58
Nuclear Periphery -2.88 -5.64 -4.52 -3.95 1.00 -2.24 -4.94 -1.65 -1.44 2.78 -3.05 -6.36 -4.80 -4.41 2.17
Nuclear Periphery Foci -2.84 -7.22 -1.47 -1.37 -1.23 -3.23 -8.04 -1.73 -1.22 -0.66 -4.07 -10.18 -1.50 -1.43 -1.34
Nucleolus -11.68 -25.63* -10.51 0.44 10.69 -4.59 -5.66 0.94 3.07 3.50 -13.57 -22.06 -8.51 0.12 5.07
Nucleus 13.24 26.85 11.36 -1.01 -10.71 3.22 6.45 -0.69 -2.08 -3.41 13.75 24.08 8.94 0.20 -5.79
Peroxisomes -4.21 -6.29 -0.74 3.59 5.49 6.77 12.90 3.75 -0.09 -3.19 1.18 7.51 4.46 3.30 -1.90
Vacuole -5.57 -6.93 -2.54 4.57 5.16 -7.83 -11.52 -0.95 0.33 9.65 -7.19 -12.43 -3.06 3.36 11.26
Vacuole Periphery -5.71 -8.84 -4.77 2.62 5.67 -5.32 -14.72 -1.24 -0.74 4.82 -7.11 -17.14 -4.84 -0.49 10.03
Standard name
Human Ortholog
Description Histone H2A; core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p
Localization
Cell Percentages nucleus (80%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-1

Hta2

Hta2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hta2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available