Standard name
Human Ortholog
Description Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; expression induced in cells treated with the mycotoxin patulin; relocalizes from nucleus to plasma membrane upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.97 0.92 0.98 0.97 0.96 0.88 0.85 0.86 – 0.76 0.67 0.69 1.0 1.0 0.99 – – – 0.84 0.72 0.67 0.75 0.79 0.72
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0.1 0 0 0 0.25 0.22 0.25 – 0.46 0.53 0.46 0 0 0 – – – 0.07 0.1 0.16 0.14 0.1 0.1
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 1 6 6 3 4 7
Bud 0 1 1 1 0 1 4 7 – 8 11 17 0 2 0 – – – 0 1 4 3 5 14
Bud Neck 2 1 1 2 3 4 5 11 – 6 1 4 0 1 0 – – – 0 1 4 2 4 6
Bud Site 0 0 0 0 0 0 0 0 – 2 2 2 0 0 0 – – – – – – – – –
Cell Periphery 1 2 3 3 3 0 2 0 – 2 3 1 7 10 0 – – – 1 0 5 4 7 8
Cytoplasm 192 141 299 240 287 290 387 453 – 349 267 296 152 296 347 – – – 172 117 195 248 406 378
Endoplasmic Reticulum 4 2 1 0 0 0 0 0 – 0 0 1 0 0 2 – – – 0 0 5 3 3 3
Endosome 0 0 1 0 2 0 2 1 – 1 0 2 0 0 1 – – – 3 3 2 2 2 5
Golgi 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 1 2 1 3
Mitochondria 1 15 7 8 10 84 99 132 – 213 209 199 0 0 0 – – – 14 16 46 45 52 54
Nucleus 0 0 0 1 2 1 2 2 – 3 1 2 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 1 1 0 0 0 1 0 0 – 1 0 0 0 0 0 – – – 0 1 1 0 0 1
Nucleolus 0 0 0 0 1 0 0 0 – 1 2 1 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 – 0 0 0 0 0 0 – – – 1 0 0 0 0 0
SpindlePole 0 0 0 2 1 2 9 9 – 13 8 19 0 0 0 – – – 2 2 5 3 7 13
Vac/Vac Membrane 0 0 1 0 2 0 4 0 – 5 2 0 0 0 2 – – – 1 4 2 5 8 12
Unique Cell Count 197 154 306 247 299 331 455 525 459 396 428 152 297 351 204 163 292 333 514 524
Labelled Cell Count 202 163 314 257 312 383 514 615 604 506 544 159 309 352 204 163 292 333 514 524


Cell Periphery, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 3.6 3.1 3.0 3.2 2.5 2.5 2.8 – 2.2 2.3 2.3 4.6 4.8 5.1 3.2 3.7 4.4 4.8 4.8 5.1
Std Deviation (1e-4) 0.5 1.0 0.7 0.9 1.5 0.9 1.1 1.3 – 0.8 0.7 0.9 0.7 0.8 1.6 0.7 0.9 2.0 1.4 1.0 1.6
Intensity Change (Log2) – – – -0.04 0.08 -0.27 -0.27 -0.13 – -0.46 -0.41 -0.39 0.6 0.64 0.75 0.06 0.26 0.54 0.64 0.64 0.74


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0043 0.317 0.3176 0.1043 0.287 0.1805 -0.7618 -0.3437 -0.5884 -0.4018 -0.4121 -0.4913 -1.3191 -0.6785 -1.1222 -0.8551 -1.4852 -0.986
Actin 0.032 0.0003 0.0256 0.0228 0.0182 0.0081 0.0202 0 0.0011 0.0009 0.0051 0.0029 0.0158 0.0002 0.0002 0.0003 0 0.0003
Bud 0.0021 0.0011 0.0051 0.0228 0.0005 0.0017 0.0023 0 0.0041 0.0002 0.0058 0.0015 0.0002 0.0003 0.0005 0.0018 0 0.0001
Bud Neck 0.0048 0.0002 0.0026 0.009 0.0035 0.0023 0.0009 0 0.0001 0.0004 0.0004 0.0003 0.0075 0.0002 0.0001 0.0004 0 0.0007
Bud Periphery 0.0078 0.0005 0.0075 0.0154 0.001 0.0033 0.0101 0 0.0123 0.0004 0.0097 0.0113 0.0003 0.0006 0.0015 0.0017 0 0.0001
Bud Site 0.004 0.011 0.0083 0.038 0.0034 0.0005 0.0054 0 0.0029 0.0005 0.002 0.0001 0.0017 0.0024 0.0001 0.0006 0 0.0009
Cell Periphery 0.0016 0.0002 0.0004 0.0008 0.0003 0.0001 0.0005 0.0001 0.0003 0 0.0002 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0
Cytoplasm 0.0349 0.0146 0.0112 0.0189 0.0048 0.0253 0.038 0.0109 0.0137 0.0047 0.0299 0.0325 0.0184 0.0343 0.0242 0.0211 0.0494 0.0422
Cytoplasmic Foci 0.0262 0.003 0.0065 0.0199 0.0036 0.0051 0.049 0.0012 0.0044 0.0323 0.0053 0.0034 0.0117 0.0074 0.0026 0.0021 0.0037 0.0069
Eisosomes 0.0008 0.0001 0.0005 0.0003 0.0005 0.0001 0.0003 0.0001 0.0007 0.0001 0.0001 0.0001 0.0006 0.0004 0.0004 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0078 0.0002 0.0013 0.0037 0.0016 0.0008 0.002 0.0004 0.0005 0.0001 0.0026 0.0019 0.0004 0.0003 0.0005 0.0003 0.0005 0.0002
Endosome 0.018 0.0005 0.0031 0.0152 0.0055 0.0021 0.0208 0.0001 0.0123 0.0056 0.0287 0.0009 0.0035 0.001 0.0005 0.0007 0.0008 0.0003
Golgi 0.0022 0.0001 0.0027 0.003 0.0023 0.0012 0.0151 0 0.0055 0.0054 0.0022 0.0005 0.002 0.0007 0.0001 0.0001 0 0.0001
Lipid Particles 0.0057 0.0001 0.0037 0.019 0.0019 0.0011 0.0079 0.0001 0.0007 0.0033 0.0033 0.0009 0.0042 0.0019 0.0004 0.0002 0.0003 0.0003
Mitochondria 0.0023 0.0002 0.0043 0.003 0.0012 0.0009 0.0133 0.0001 0.0018 0.008 0.0438 0.0057 0.0015 0.0008 0.0003 0.0004 0.0001 0.0002
None 0.761 0.9582 0.8951 0.7568 0.9412 0.9346 0.7562 0.9864 0.9347 0.8863 0.8022 0.9236 0.9088 0.9306 0.9676 0.9646 0.9444 0.9465
Nuclear Periphery 0.0228 0.0002 0.0036 0.0078 0.0035 0.0022 0.003 0.0001 0.0005 0.0001 0.0114 0.0011 0.0002 0.0001 0.0001 0.0004 0.0001 0.0001
Nucleolus 0.0033 0.0006 0.0012 0.0038 0.0007 0.0004 0.0176 0 0.0003 0.0004 0.0005 0.0001 0.0002 0.0002 0.0001 0.0004 0 0.0001
Nucleus 0.0206 0.0031 0.0053 0.0168 0.0017 0.004 0.0074 0.0001 0.0025 0.0002 0.0135 0.0005 0.0002 0.0002 0.0002 0.0014 0.0001 0.0001
Peroxisomes 0.0055 0.0001 0.0036 0.0088 0.0009 0.0007 0.0042 0 0.0004 0.0471 0.0151 0.0107 0.0214 0.0174 0.0001 0.0001 0 0.0003
Punctate Nuclear 0.0256 0.0055 0.0072 0.0108 0.0027 0.0032 0.0228 0.0001 0.0006 0.0037 0.0016 0.0011 0.0009 0.0003 0.0002 0.0024 0.0002 0.0004
Vacuole 0.0093 0.0002 0.0008 0.0025 0.0007 0.0015 0.0022 0.0001 0.0004 0.0002 0.0131 0.0004 0.0003 0.0004 0.0002 0.0007 0.0003 0.0001
Vacuole Periphery 0.0016 0 0.0004 0.0008 0.0004 0.0006 0.0008 0 0.0001 0.0001 0.0034 0.0001 0.0001 0 0 0.0001 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.7339 11.379 7.9993 6.8314 12.5949 8.0349 13.9274 10.4218 9.2802 12.5018
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
21 1527 161 1597 116 901 2712 1493 137 2428 2873 3090

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 845.59 678.27 832.01 791.16 641.20 679.65 760.69 789.45 672.53 678.78 764.69 790.33
Standard Deviation 91.41 94.88 111.56 109.42 74.38 83.51 93.87 105.07 106.71 90.83 96.36 107.35
Intensity Change Log 2 — -0.318099 -0.023357 -0.095989 — 0.084018 0.246534 0.300073 — -0.130802 0.099274 0.088281

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001051 0.000243 0.000997 0.000617 0.000090 0.000180 0.000095 0.000450 0.000237 0.000220 0.000146 0.000536
Bud Neck 0.013281 0.028155 0.001394 0.008404 0.026390 0.042208 0.010790 0.013311 0.024381 0.033370 0.010263 0.010775
Bud Site 0.024827 0.011296 0.003083 0.034488 0.001579 0.016365 0.019789 0.030596 0.005143 0.013177 0.018853 0.032608
Cell Periphery 0.000444 0.000146 0.000261 0.000245 0.000211 0.000807 0.000132 0.000208 0.000247 0.000391 0.000139 0.000227
Cytoplasm 0.218678* 0.453042* 0.400472* 0.440419* 0.367968* 0.345119* 0.526099* 0.461722* 0.345084* 0.412993* 0.519059* 0.450712*
Cytoplasmic Foci 0.182118* 0.242889* 0.027862 0.021696 0.316426* 0.265083* 0.009794 0.030806 0.295839* 0.251125* 0.010807 0.026098
Eisosomes 0.000276 0.000157 0.000090 0.000051 0.000304 0.000284 0.000022 0.000060 0.000299 0.000204 0.000026 0.000055
Endoplasmic Reticulum 0.001073 0.000516 0.006748 0.002056 0.000367 0.001377 0.000595 0.001973 0.000475 0.000836 0.000939 0.002016
Endosome 0.051438 0.016853 0.004888 0.004242 0.013302 0.028639 0.002234 0.007045 0.019148 0.021226 0.002382 0.005596
Golgi 0.006640 0.002924 0.000205 0.001120 0.001316 0.005783 0.000300 0.004685 0.002132 0.003985 0.000295 0.002842
Lipid Particles 0.007759 0.007318 0.003593 0.001504 0.012764 0.022506 0.000476 0.002215 0.011997 0.012954 0.000651 0.001848
Mitochondria 0.000880 0.010210 0.000288 0.004186 0.005723 0.015383 0.001739 0.005690 0.004980 0.012130 0.001658 0.004913
Mitotic Spindle 0.000895 0.004142 0.001549 0.039794 0.000135 0.002927 0.011047 0.025526 0.000252 0.003691 0.010515 0.032900
None 0.000415 0.002775 0.003838 0.004378 0.001361 0.003178 0.003650 0.003974 0.001216 0.002924 0.003661 0.004183
Nuclear Periphery 0.000622 0.000399 0.001154 0.001881 0.000386 0.000858 0.001062 0.001584 0.000422 0.000569 0.001067 0.001738
Nuclear Periphery Foci 0.000491 0.000328 0.004644 0.000947 0.000282 0.001068 0.000207 0.000826 0.000314 0.000602 0.000456 0.000889
Nucleolus 0.000726 0.001452 0.000924 0.000607 0.000988 0.001919 0.000454 0.000654 0.000948 0.001625 0.000480 0.000630
Nucleus 0.149064* 0.131481* 0.460181* 0.324243* 0.202024* 0.162604* 0.376835* 0.294516* 0.193906* 0.143030* 0.381505* 0.309880*
Peroxisomes 0.013375 0.012016 0.001399 0.000598 0.018611 0.016378 0.000324 0.001524 0.017809 0.013634 0.000385 0.001046
Vacuole 0.322960* 0.072319 0.076205 0.105941 0.029312 0.064288 0.033682 0.109572 0.074324 0.069339 0.036065 0.107695
Vacuole Periphery 0.002987 0.001340 0.000228 0.002582 0.000458 0.003046 0.000673 0.003062 0.000846 0.001973 0.000648 0.002814

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.11 -0.25 0.77 -2.99 1.64 -2.04 0.01 -4.33 -2.86 -4.77 0.05 0.70 -1.69 -3.94 -5.21
Bud Neck -3.67 2.06 0.75 10.53 -8.44 -1.64 1.73 1.74 9.32 0.11 -1.18 1.79 1.91 13.71 0.88
Bud Site 1.10 1.67 0.30 -4.03 -7.44 -5.48 -8.76 -8.20 -0.89 -1.02 -3.23 -4.09 -5.81 -3.70 -2.02
Cell Periphery 2.25 0.74 1.95 -0.88 3.32 -1.59 4.04 2.72 1.78 -1.79 -1.06 4.23 2.82 1.67 -1.67
Cytoplasm -4.33 -2.26 -2.55 9.15 -0.26 0.81 -5.23 -0.87 -3.67 11.24 -3.13 -6.23 -1.31 5.33 14.66
Cytoplasmic Foci -2.25 3.87 4.16 39.77 2.23 2.52 15.76 14.95 32.17 -9.94 2.34 15.74 15.14 50.84 -9.97
Eisosomes 1.68 2.46 3.60 14.80 4.63 0.58 7.11 6.08 9.88 -17.30 2.67 7.74 6.81 15.28 -17.68
Endoplasmic Reticulum 2.07 -6.66 -6.52 -18.02 5.10 -1.80 -4.93 -9.44 -1.77 -7.81 -1.57 -6.78 -15.70 -8.03 -10.33
Endosome 1.10 1.59 1.72 13.23 3.11 -3.89 3.39 2.65 11.57 -4.05 -0.78 3.46 3.10 16.96 -4.09
Golgi 0.54 1.15 1.08 6.59 -1.53 -4.58 3.12 -0.94 3.07 -2.47 -2.43 2.34 0.78 4.87 -2.71
Lipid Particles -0.30 0.81 1.91 9.61 3.41 -2.97 5.21 4.61 9.88 -7.10 -0.39 5.44 4.93 12.81 -8.86
Mitochondria -6.17 0.88 -2.35 4.93 -3.92 -2.34 1.10 0.70 6.75 -2.08 -2.08 1.08 0.68 7.90 -2.36
Mitotic Spindle -2.32 -0.25 -8.55 -7.36 -8.22 -2.49 -7.82 -7.41 -5.58 -2.45 -4.21 -7.73 -11.62 -9.15 -5.36
None -4.69 -6.76 -5.85 -1.45 -0.73 -2.39 -4.83 -4.87 -0.76 -0.97 -3.35 -5.97 -5.50 -1.71 -1.05
Nuclear Periphery 0.76 -3.82 -10.63 -18.76 -10.05 -2.92 -16.10 -17.55 -8.76 -9.69 -1.99 -15.33 -23.36 -20.71 -15.03
Nuclear Periphery Foci 0.82 -5.58 -2.93 -6.02 4.89 -1.38 0.43 -4.11 0.31 -7.44 -1.17 -1.82 -5.38 -2.17 -4.53
Nucleolus -3.27 -1.18 -0.02 5.27 1.74 -2.32 2.47 2.00 5.09 -1.67 -2.55 2.42 1.88 7.40 -2.02
Nucleus 0.90 -4.94 -3.27 -26.82 4.70 2.36 -12.20 -7.47 -18.42 8.74 3.48 -12.84 -8.76 -33.00 9.49
Peroxisomes -0.08 1.70 1.88 12.44 1.27 0.51 3.42 3.31 10.21 -3.77 0.93 3.70 3.64 15.93 -2.72
Vacuole 3.99 3.26 2.09 -17.40 -5.49 -6.36 -4.01 -21.27 -17.22 -21.72 0.18 1.67 -8.27 -24.27 -29.42
Vacuole Periphery 1.10 2.24 1.07 0.15 -2.15 -6.21 -0.56 -2.14 2.55 -1.92 -4.90 1.08 -1.62 1.63 -2.36
Standard name
Human Ortholog
Description Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; expression induced in cells treated with the mycotoxin patulin; relocalizes from nucleus to plasma membrane upon DNA replication stress
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Flr1

Flr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Flr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available