Standard name | |
---|---|
Human Ortholog | |
Description | Alpha-1,2-mannosyltransferase; responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 4 | 5 | 2 | 2 | 4 | 3 | 0 | 9 | 5 | 3 | 2 | 0 | 58 | 55 | 25 | 1 | 2 | 0 | 2 | 1 | 3 | 0 | 8 | 3 |
Bud | 1 | 1 | 2 | 5 | 7 | 9 | 10 | 27 | 15 | 20 | 26 | 20 | 11 | 14 | 13 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 0 | 1 | 1 | 1 | 0 | 2 | 0 | 2 | 1 | 2 | 1 | 2 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 24 | 53 | 29 | 27 | 35 | 44 | 60 | 68 | 43 | 80 | 63 | 66 | 91 | 270 | 378 | 93 | 15 | 18 | 0 | 1 | 0 | 2 | 2 | 5 |
Endoplasmic Reticulum | 5 | 15 | 0 | 5 | 5 | 4 | 8 | 6 | 5 | 4 | 3 | 2 | 80 | 85 | 30 | 12 | 1 | 4 | 0 | 0 | 2 | 2 | 7 | 3 |
Endosome | 31 | 6 | 89 | 93 | 110 | 87 | 86 | 174 | 59 | 79 | 36 | 95 | 172 | 103 | 153 | 28 | 12 | 10 | 46 | 60 | 104 | 86 | 135 | 86 |
Golgi | 8 | 18 | 21 | 35 | 21 | 12 | 14 | 22 | 10 | 3 | 4 | 3 | 33 | 2 | 2 | 5 | 2 | 8 | 51 | 61 | 119 | 70 | 126 | 75 |
Mitochondria | 40 | 48 | 65 | 70 | 58 | 110 | 121 | 183 | 144 | 141 | 154 | 142 | 23 | 2 | 4 | 17 | 2 | 5 | 8 | 14 | 8 | 26 | 71 | 48 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 6 | 1 | 3 | 0 | 5 | 0 | 1 | 2 | 12 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 1 | 3 | 2 | 2 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Nucleolus | 1 | 0 | 1 | 6 | 7 | 7 | 12 | 18 | 5 | 12 | 11 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 1 | 3 | 8 | 6 | 10 | 4 | 15 | 3 | 10 | 10 | 12 | 0 | 1 | 3 | 1 | 0 | 0 | 0 | 1 | 6 | 7 | 3 | 1 |
SpindlePole | 2 | 1 | 5 | 9 | 11 | 12 | 6 | 27 | 12 | 9 | 8 | 17 | 30 | 38 | 61 | 2 | 2 | 1 | 1 | 0 | 1 | 0 | 0 | 0 |
Vac/Vac Membrane | 62 | 37 | 77 | 33 | 59 | 52 | 62 | 88 | 52 | 71 | 59 | 68 | 191 | 99 | 100 | 181 | 52 | 68 | 45 | 53 | 29 | 41 | 184 | 248 |
Unique Cell Count | 139 | 144 | 235 | 217 | 247 | 272 | 313 | 501 | 289 | 345 | 307 | 353 | 580 | 596 | 697 | 254 | 77 | 94 | 159 | 199 | 278 | 239 | 547 | 479 |
Labelled Cell Count | 182 | 185 | 296 | 295 | 327 | 355 | 387 | 646 | 357 | 437 | 383 | 440 | 692 | 678 | 779 | 354 | 93 | 117 | 159 | 199 | 278 | 239 | 547 | 479 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.4 | 6.7 | 6.8 | 7.5 | 7.5 | 7.0 | 6.8 | 6.8 | 6.7 | 6.3 | 6.5 | 6.4 | 7.0 | 6.2 | 6.0 | 6.4 | 6.8 | 9.0 | 7.6 | 7.5 | 7.5 |
Std Deviation (1e-4) | 0.9 | 1.1 | 1.4 | 1.5 | 2.0 | 1.5 | 1.4 | 1.4 | 1.3 | 1.2 | 1.2 | 1.0 | 0.7 | 0.6 | 0.7 | 1.4 | 1.5 | 2.3 | 1.3 | 1.5 | 1.3 |
Intensity Change (Log2) | – | – | – | 0.14 | 0.14 | 0.03 | 0.0 | -0.01 | -0.03 | -0.12 | -0.07 | -0.1 | 0.03 | -0.14 | -0.18 | -0.09 | 0.0 | 0.4 | 0.16 | 0.14 | 0.14 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.5 | 4.3 | 2.2 | 0 | 0 | 0 |
Bud | 0 | 0 | 1.9 | 1.9 | 3.0 | 2.8 | 3.1 | 4.0 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0.6 | 1.2 | 2.1 | 0.5 | 0.8 | 3.3 | 2.5 | 2.1 | 1.2 | 8.9 | 11.2 | 6.2 | 1.6 | 1.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.0 | 6.1 | 3.2 | 3.4 | 0 | 0 |
Endosome | 1.1 | 1.5 | -1.4 | -2.6 | -0.8 | -4.4 | -3.9 | -7.2 | -2.8 | -2.3 | -6.3 | -4.8 | -7.0 | -3.6 | -4.9 |
Golgi | 2.3 | -0.2 | -2.1 | -2.1 | -2.5 | -2.6 | -4.8 | -4.2 | -4.9 | -1.7 | -6.8 | -7.4 | -3.4 | -1.8 | -0.1 |
Mitochondria | 1.1 | -1.1 | 3.0 | 2.7 | 2.4 | 5.2 | 3.3 | 5.3 | 3.1 | -9.9 | -13.0 | -13.7 | -6.2 | -4.6 | -4.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 2.6 | 2.5 | 0 | 2.4 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1.7 | 0 | 1.7 | 0 | 1.4 | 0 | 1.3 | 1.5 | 1.6 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1.2 | 1.4 | 1.4 | 0 | 2.0 | 1.3 | 0.4 | 0.4 | 1.7 | 1.9 | 2.5 | 3.4 | 0 | 0 | 0 |
Vacuole | -4.3 | -2.2 | -3.5 | -3.5 | -4.6 | -3.9 | -3.3 | -3.6 | -3.7 | 0 | -5.1 | -6.2 | 8.5 | 5.4 | 6.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.754 | 4.4869 | 3.7545 | 3.3893 | 3.5147 | 4.2534 | 4.925 | 7.2072 | 7.1294 | 5.5307 | 6.8158 | 7.2505 | 5.4644 | 7.3352 | 6.3459 | 6.4333 | 7.0561 | 6.6537 |
Actin | 0.0187 | 0.0001 | 0.0003 | 0 | 0 | 0.0002 | 0.0086 | 0.0014 | 0.0006 | 0.0001 | 0 | 0.0005 | 0.0006 | 0.0003 | 0.0023 | 0.0001 | 0 | 0.0004 |
Bud | 0.0006 | 0.0002 | 0.0002 | 0.0017 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Bud Neck | 0.002 | 0.0007 | 0.0002 | 0.0007 | 0.0002 | 0.0005 | 0.0002 | 0.0005 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0003 |
Bud Periphery | 0.0009 | 0.0003 | 0.0002 | 0.0013 | 0.0001 | 0.0003 | 0.0006 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 |
Bud Site | 0.0042 | 0.0005 | 0.0003 | 0.0113 | 0.0003 | 0.0003 | 0.0002 | 0.0005 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0002 |
Cell Periphery | 0.0004 | 0.0004 | 0.0001 | 0.0022 | 0.0001 | 0.0007 | 0.0003 | 0.0009 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0002 |
Cytoplasm | 0.0102 | 0.0168 | 0.0042 | 0.0043 | 0.0024 | 0.0093 | 0.0075 | 0.0045 | 0.0019 | 0.0031 | 0.0013 | 0.0046 | 0.0049 | 0.003 | 0.003 | 0.0024 | 0.0027 | 0.0025 |
Cytoplasmic Foci | 0.0273 | 0.0316 | 0.0332 | 0.019 | 0.0209 | 0.0298 | 0.0429 | 0.0467 | 0.0367 | 0.0514 | 0.0321 | 0.0218 | 0.0405 | 0.0501 | 0.0343 | 0.01 | 0.0246 | 0.0251 |
Eisosomes | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0016 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0115 | 0.0051 | 0.002 | 0.0017 | 0.0012 | 0.0023 | 0.0055 | 0.0019 | 0.0035 | 0.0008 | 0.0007 | 0.0017 | 0.008 | 0.0019 | 0.0033 | 0.0023 | 0.0015 | 0.0038 |
Endosome | 0.5134 | 0.5547 | 0.6251 | 0.6308 | 0.5778 | 0.5412 | 0.5124 | 0.5353 | 0.4711 | 0.582 | 0.593 | 0.3957 | 0.5474 | 0.5069 | 0.5705 | 0.4584 | 0.574 | 0.5073 |
Golgi | 0.0919 | 0.0801 | 0.1504 | 0.0687 | 0.0746 | 0.147 | 0.333 | 0.2672 | 0.3871 | 0.2384 | 0.1787 | 0.3609 | 0.2263 | 0.2802 | 0.1954 | 0.1017 | 0.2198 | 0.2349 |
Lipid Particles | 0.0198 | 0.0065 | 0.0057 | 0.0027 | 0.0032 | 0.0098 | 0.0101 | 0.0103 | 0.0075 | 0.0116 | 0.0053 | 0.0241 | 0.0087 | 0.0141 | 0.0053 | 0.0037 | 0.0312 | 0.0089 |
Mitochondria | 0.0296 | 0.0088 | 0.0313 | 0.0031 | 0.0032 | 0.0165 | 0.0227 | 0.0441 | 0.0572 | 0.0157 | 0.0031 | 0.0207 | 0.0169 | 0.0084 | 0.0453 | 0.0127 | 0.0032 | 0.0346 |
None | 0.0092 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0004 | 0.0155 | 0.0086 | 0.0001 | 0.0002 | 0 | 0.0033 | 0.012 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0001 |
Nuclear Periphery | 0.0309 | 0.0076 | 0.0029 | 0.0026 | 0.0016 | 0.0029 | 0.0045 | 0.0007 | 0.0024 | 0.001 | 0.0006 | 0.0031 | 0.0035 | 0.0015 | 0.0016 | 0.0024 | 0.0014 | 0.0045 |
Nucleolus | 0.0023 | 0.0003 | 0.0001 | 0.0027 | 0.0002 | 0.0008 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0002 |
Nucleus | 0.0131 | 0.0014 | 0.0005 | 0.0016 | 0.0005 | 0.0015 | 0.0006 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0005 | 0.0005 | 0.0002 | 0.0004 | 0.0003 | 0.0004 |
Peroxisomes | 0.0042 | 0.0008 | 0.001 | 0.0003 | 0.0004 | 0.0009 | 0.004 | 0.0035 | 0.0011 | 0.0015 | 0.0006 | 0.002 | 0.0016 | 0.0026 | 0.0121 | 0.0003 | 0.0004 | 0.0013 |
Punctate Nuclear | 0.0067 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0005 | 0.0011 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 |
Vacuole | 0.1263 | 0.2091 | 0.093 | 0.169 | 0.2305 | 0.1618 | 0.0151 | 0.0457 | 0.0145 | 0.046 | 0.1179 | 0.0883 | 0.0808 | 0.0755 | 0.0814 | 0.1891 | 0.0948 | 0.0944 |
Vacuole Periphery | 0.0764 | 0.0746 | 0.0487 | 0.076 | 0.0824 | 0.0731 | 0.0133 | 0.0267 | 0.0156 | 0.0462 | 0.0658 | 0.0718 | 0.047 | 0.0536 | 0.0444 | 0.2158 | 0.0446 | 0.0806 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 127.931 | 88.5209 | 44.8789 | 60.9485 | 84.5289 | 65.1595 | 81.7205 | 61.9096 | 60.7155 | 95.58 |
Translational Efficiency | 0.909 | 0.9289 | 1.1287 | 0.8656 | 0.9716 | 1.4623 | 0.8163 | 0.7935 | 0.8 | 0.7752 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
180 | 1094 | 84 | 79 | 1368 | 1841 | 256 | 1698 | 1548 | 2935 | 340 | 1777 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 955.70 | 887.09 | 1052.93 | 940.24 | 814.99 | 895.51 | 1073.88 | 925.69 | 831.35 | 892.37 | 1068.70 | 926.34 |
Standard Deviation | 118.74 | 127.51 | 102.39 | 111.18 | 525.97 | 120.12 | 117.66 | 120.92 | 498.15 | 122.99 | 114.43 | 120.54 |
Intensity Change Log 2 | — | -0.107477 | 0.139780 | -0.023529 | — | 0.135927 | 0.397979 | 0.183747 | — | 0.009671 | 0.264379 | 0.075583 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.012908 | 0.001199 | 0.002439 | 0.016783 | 0.002129 | 0.001271 | 0.003219 | 0.010811 | 0.003382 | 0.001244 | 0.003026 | 0.011077 |
Bud Neck | 0.005878 | 0.000278 | 0.001470 | 0.003518 | 0.000525 | 0.000688 | 0.000738 | 0.003842 | 0.001147 | 0.000535 | 0.000919 | 0.003828 |
Bud Site | 0.031246 | 0.003383 | 0.012669 | 0.047745 | 0.005250 | 0.001245 | 0.008674 | 0.021120 | 0.008273 | 0.002042 | 0.009661 | 0.022303 |
Cell Periphery | 0.000196 | 0.000038 | 0.000072 | 0.000146 | 0.000103 | 0.000057 | 0.000042 | 0.000059 | 0.000114 | 0.000050 | 0.000049 | 0.000062 |
Cytoplasm | 0.098030 | 0.002770 | 0.006896 | 0.030043 | 0.005189 | 0.001644 | 0.002628 | 0.031262 | 0.015984 | 0.002064 | 0.003683 | 0.031208 |
Cytoplasmic Foci | 0.207290* | 0.241719* | 0.430510* | 0.280759* | 0.182458* | 0.159188 | 0.387630* | 0.277879* | 0.185345* | 0.189950* | 0.398224* | 0.278007* |
Eisosomes | 0.000062 | 0.000032 | 0.000038 | 0.000094 | 0.000044 | 0.000053 | 0.000035 | 0.000064 | 0.000046 | 0.000045 | 0.000036 | 0.000065 |
Endoplasmic Reticulum | 0.000893 | 0.000175 | 0.005348 | 0.004699 | 0.000321 | 0.000231 | 0.000554 | 0.001271 | 0.000388 | 0.000210 | 0.001738 | 0.001423 |
Endosome | 0.164599 | 0.250374* | 0.288782* | 0.265107* | 0.301267* | 0.320165* | 0.265806* | 0.303465* | 0.285376* | 0.294151* | 0.271482* | 0.301760* |
Golgi | 0.038599 | 0.442394* | 0.096399 | 0.087069 | 0.440653* | 0.457850* | 0.232034* | 0.189888* | 0.393902* | 0.452089* | 0.198524* | 0.185317* |
Lipid Particles | 0.002477 | 0.003812 | 0.010899 | 0.015424 | 0.006939 | 0.007327 | 0.010713 | 0.009106 | 0.006420 | 0.006017 | 0.010759 | 0.009387 |
Mitochondria | 0.008449 | 0.013226 | 0.000282 | 0.006909 | 0.016658 | 0.017288 | 0.001090 | 0.005871 | 0.015703 | 0.015774 | 0.000890 | 0.005917 |
Mitotic Spindle | 0.010309 | 0.005636 | 0.064754 | 0.022933 | 0.000674 | 0.000700 | 0.032768 | 0.016650 | 0.001794 | 0.002540 | 0.040670 | 0.016929 |
None | 0.000060 | 0.000056 | 0.000009 | 0.000085 | 0.000526 | 0.000167 | 0.000005 | 0.000031 | 0.000472 | 0.000125 | 0.000006 | 0.000033 |
Nuclear Periphery | 0.000262 | 0.000026 | 0.000136 | 0.000261 | 0.000053 | 0.000097 | 0.000236 | 0.000150 | 0.000077 | 0.000071 | 0.000211 | 0.000155 |
Nuclear Periphery Foci | 0.000960 | 0.000187 | 0.002398 | 0.004759 | 0.000255 | 0.000425 | 0.002585 | 0.000918 | 0.000337 | 0.000336 | 0.002539 | 0.001088 |
Nucleolus | 0.000182 | 0.000072 | 0.000061 | 0.000183 | 0.000250 | 0.000264 | 0.000059 | 0.000223 | 0.000242 | 0.000193 | 0.000060 | 0.000222 |
Nucleus | 0.007682 | 0.000079 | 0.000456 | 0.006622 | 0.003816 | 0.000106 | 0.000262 | 0.000879 | 0.004265 | 0.000096 | 0.000310 | 0.001134 |
Peroxisomes | 0.062684 | 0.007981 | 0.019140 | 0.065799 | 0.012051 | 0.006815 | 0.022457 | 0.039781 | 0.017939 | 0.007250 | 0.021637 | 0.040937 |
Vacuole | 0.341278* | 0.016177 | 0.049735 | 0.136024 | 0.011726 | 0.015358 | 0.025695 | 0.079591 | 0.050046 | 0.015663 | 0.031634 | 0.082100 |
Vacuole Periphery | 0.005955 | 0.010387 | 0.007507 | 0.005038 | 0.009115 | 0.009062 | 0.002771 | 0.007141 | 0.008747 | 0.009556 | 0.003941 | 0.007047 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.58 | 1.33 | -0.61 | -2.08 | -1.87 | 1.57 | -1.63 | -5.71 | -6.41 | -4.75 | 2.29 | -0.13 | -4.98 | -6.79 | -5.25 |
Bud Neck | 5.19 | 3.88 | 1.81 | -6.00 | -3.37 | -0.65 | -1.93 | -6.52 | -5.93 | -5.85 | 2.90 | 0.53 | -5.56 | -6.64 | -5.80 |
Bud Site | 4.08 | 2.19 | -0.86 | -2.49 | -1.83 | 3.07 | -1.19 | -7.55 | -11.68 | -2.96 | 4.18 | -0.90 | -6.57 | -11.23 | -3.58 |
Cell Periphery | 1.33 | 0.92 | 0.12 | -3.11 | -1.86 | 2.53 | 3.48 | 2.73 | -0.14 | -1.73 | 3.24 | 3.18 | 2.65 | -1.46 | -1.33 |
Cytoplasm | 8.15 | 7.64 | 3.80 | -3.83 | -3.32 | 3.32 | 1.20 | -11.87 | -14.17 | -12.70 | 7.73 | 5.91 | -6.23 | -14.54 | -12.61 |
Cytoplasmic Foci | -4.71 | -9.50 | -3.01 | -0.33 | 5.74 | 4.93 | -14.83 | -14.98 | -20.56 | 8.14 | -1.82 | -17.83 | -15.32 | -15.45 | 10.20 |
Eisosomes | 4.02 | 2.24 | -2.36 | -4.24 | -3.54 | -1.15 | 1.49 | -4.07 | -1.36 | -5.19 | 0.09 | 1.93 | -4.28 | -3.57 | -5.94 |
Endoplasmic Reticulum | 4.62 | -1.49 | -1.92 | -2.33 | 0.15 | 0.95 | -1.78 | -3.14 | -3.58 | -1.28 | 2.21 | -2.00 | -3.43 | -4.20 | 0.61 |
Endosome | -7.96 | -6.64 | -3.38 | 1.00 | 1.96 | -2.49 | 3.06 | 1.33 | 3.85 | -2.26 | -2.01 | 1.14 | -0.91 | 0.98 | -1.75 |
Golgi | -42.48* | -4.82 | -2.51 | 21.30 | 1.20 | -2.08 | 15.98 | 31.63 | 35.36* | 3.71 | -7.53 | 16.72 | 26.13 | 40.59 | 1.83 |
Lipid Particles | -1.77 | -5.03 | -3.93 | -3.47 | -1.04 | -0.23 | -1.22 | -1.55 | -1.34 | 0.54 | 0.33 | -1.76 | -2.26 | -3.14 | 0.60 |
Mitochondria | -1.36 | 1.16 | 0.06 | 1.40 | -1.05 | -0.29 | 8.26 | 5.71 | 7.96 | -5.34 | -0.18 | 8.33 | 5.33 | 8.16 | -6.11 |
Mitotic Spindle | 1.31 | -2.94 | -1.45 | -2.36 | 2.20 | -0.12 | -4.09 | -9.32 | -9.31 | 1.97 | -0.88 | -5.23 | -8.82 | -8.39 | 3.02 |
None | 1.51 | 3.04 | -0.77 | -1.22 | -1.59 | 1.57 | 2.75 | 2.59 | 0.91 | -6.89 | 1.90 | 2.82 | 2.59 | 0.90 | -5.27 |
Nuclear Periphery | 3.24 | 2.41 | 0.54 | -5.00 | -3.29 | -0.65 | -2.25 | -3.87 | -1.47 | 1.00 | 0.70 | -1.88 | -2.70 | -2.74 | 0.72 |
Nuclear Periphery Foci | 5.67 | -1.48 | -2.22 | -2.76 | -1.72 | -0.75 | -2.26 | -2.25 | -1.57 | 1.66 | 0.50 | -2.64 | -2.59 | -2.72 | 1.72 |
Nucleolus | 2.78 | 3.71 | -0.04 | -2.35 | -2.93 | -0.02 | 1.37 | 0.04 | 0.04 | -2.41 | 0.35 | 1.52 | 0.00 | -0.42 | -2.59 |
Nucleus | 3.46 | 3.34 | 0.39 | -3.14 | -3.02 | 3.01 | 2.51 | 2.01 | -5.15 | -1.68 | 3.99 | 3.49 | 2.56 | -6.05 | -2.98 |
Peroxisomes | 4.00 | 2.72 | -0.43 | -3.41 | -2.53 | 3.50 | -1.98 | -9.52 | -12.37 | -2.99 | 5.09 | -1.24 | -7.88 | -12.80 | -3.97 |
Vacuole | 15.12 | 12.95 | 4.71 | -6.06 | -4.82 | -1.86 | -3.19 | -18.81 | -17.92 | -8.53 | 8.99 | 3.05 | -6.99 | -18.92 | -9.50 |
Vacuole Periphery | -1.83 | -0.70 | 0.05 | 2.51 | 0.85 | 0.04 | 5.20 | 1.98 | 2.34 | -4.08 | -0.89 | 3.48 | 1.68 | 3.20 | -2.41 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|