Standard name | |
---|---|
Human Ortholog | |
Description | Acyltransferase; enzyme mainly responsible for the introduction of saturated very long chain fatty acids into neo-synthesized molecules of phosphatidylinositol; required for incorporation of stearic acid into phosphatidylinositol; affects chromosome stability when overexpressed; CST26 has a paralog, YDR018C, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 8 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 8 | 3 | 1 | 2 | 0 | 18 | 42 | 23 | 9 | 20 | 21 |
Bud | 2 | 7 | 11 | 12 | 26 | 35 | 26 | 35 | 20 | 23 | 20 | 23 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 2 | 2 | 3 | 6 |
Bud Neck | 0 | 0 | 2 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 3 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 4 | 5 | 6 | 5 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 1 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 40 | 61 | 59 | 60 | 129 | 132 | 79 | 81 | 47 | 53 | 38 | 31 | 22 | 24 | 29 | 13 | 22 | 19 | 16 | 12 | 7 | 10 | 17 | 21 |
Endoplasmic Reticulum | 14 | 46 | 6 | 2 | 1 | 10 | 13 | 3 | 9 | 5 | 6 | 4 | 25 | 21 | 8 | 1 | 9 | 5 | 6 | 6 | 8 | 6 | 4 | 9 |
Endosome | 138 | 133 | 89 | 102 | 103 | 29 | 26 | 37 | 13 | 37 | 11 | 7 | 73 | 71 | 91 | 131 | 100 | 109 | 248 | 214 | 159 | 82 | 125 | 114 |
Golgi | 0 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 19 | 16 | 14 | 28 | 36 | 17 | 13 | 15 | 17 |
Mitochondria | 101 | 119 | 101 | 89 | 184 | 549 | 765 | 766 | 690 | 729 | 667 | 786 | 6 | 5 | 2 | 40 | 21 | 20 | 55 | 121 | 63 | 69 | 96 | 95 |
Nucleus | 4 | 0 | 1 | 0 | 1 | 7 | 9 | 12 | 16 | 16 | 12 | 17 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 2 | 1 | 0 | 0 | 0 | 3 | 12 | 1 | 23 | 14 | 10 | 15 | 1 | 1 | 0 | 14 | 23 | 23 | 6 | 5 | 4 | 2 | 1 | 1 |
Nucleolus | 19 | 0 | 1 | 1 | 3 | 3 | 8 | 30 | 18 | 13 | 26 | 30 | 1 | 1 | 0 | 26 | 20 | 26 | 1 | 0 | 0 | 0 | 2 | 0 |
Peroxisomes | 10 | 16 | 69 | 66 | 31 | 13 | 17 | 8 | 13 | 10 | 3 | 5 | 10 | 16 | 8 | 16 | 24 | 48 | 43 | 61 | 90 | 42 | 52 | 32 |
SpindlePole | 33 | 29 | 54 | 55 | 28 | 27 | 30 | 41 | 18 | 30 | 19 | 13 | 54 | 41 | 39 | 40 | 34 | 34 | 20 | 21 | 24 | 11 | 14 | 18 |
Vac/Vac Membrane | 42 | 39 | 6 | 1 | 18 | 31 | 56 | 71 | 73 | 104 | 95 | 85 | 4 | 10 | 14 | 15 | 21 | 14 | 13 | 11 | 3 | 9 | 16 | 16 |
Unique Cell Count | 327 | 380 | 308 | 269 | 401 | 708 | 884 | 903 | 777 | 840 | 752 | 856 | 163 | 165 | 170 | 206 | 202 | 214 | 471 | 546 | 416 | 267 | 380 | 366 |
Labelled Cell Count | 405 | 463 | 406 | 388 | 526 | 846 | 1043 | 1090 | 945 | 1040 | 912 | 1023 | 200 | 204 | 198 | 316 | 292 | 313 | 471 | 546 | 416 | 267 | 380 | 366 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.9 | 5.0 | 4.4 | 4.4 | 3.9 | 4.0 | 3.6 | 4.0 | 3.9 | 4.2 | 4.2 | 4.2 | 5.7 | 5.5 | 5.5 | 8.0 | 7.7 | 7.7 | 6.3 | 6.2 | 6.1 |
Std Deviation (1e-4) | 1.0 | 0.8 | 1.3 | 1.6 | 1.4 | 1.4 | 1.2 | 1.4 | 1.1 | 1.2 | 1.1 | 1.0 | 1.0 | 1.0 | 0.9 | 1.4 | 1.4 | 1.4 | 1.8 | 1.4 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.02 | -0.17 | -0.14 | -0.28 | -0.15 | -0.18 | -0.09 | -0.07 | -0.06 | 0.37 | 0.31 | 0.33 | 0.85 | 0.79 | 0.81 | 0.51 | 0.49 | 0.47 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.9 | 3.9 | -0.2 | -4.8 | -4.8 | -6.6 | -6.5 | -7.2 | -8.8 | -1.5 | -1.3 | -0.6 | -4.1 | -2.5 | -3.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5.6 | 4.8 | 0 | 0 | 1.6 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 | 3.1 | 5.3 | 7.8 | 4.7 | 5.1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.8 | 4.4 | 3.8 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -7.2 | -7.4 | -8.0 | -3.3 | -5.8 | -6.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.6 | 6.1 | 5.9 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.1 | 5.3 | 6.0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -4.5 | -3.4 | -5.0 | -4.4 | -3.0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.8 | 1.9 | 1.4 | 0.5 | -0.2 | -0.5 |
Vacuole | 0 | 1.9 | 1.9 | 3.0 | 3.7 | 4.3 | 5.3 | 5.4 | 4.5 | 0 | 2.4 | 3.3 | 3.0 | 4.2 | 2.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.8688 | 1.6533 | 1.2092 | 0.9963 | 1.0191 | 1.2987 | 3.3872 | 4.0266 | 3.9272 | 3.8636 | 3.311 | 3.9249 | 1.1306 | 2.1475 | 1.6064 | 1.1833 | 1.384 | 1.6448 |
Actin | 0.0173 | 0.0024 | 0.013 | 0.0038 | 0.0019 | 0.0078 | 0.0208 | 0.0013 | 0.0279 | 0.0004 | 0.0011 | 0.004 | 0.0003 | 0.0006 | 0.0003 | 0.0001 | 0.0001 | 0.0001 |
Bud | 0.0003 | 0.0009 | 0.0011 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0007 | 0.0011 | 0.0005 | 0.0002 | 0.0003 | 0.0005 | 0.0026 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0008 |
Bud Periphery | 0.0004 | 0.0005 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0004 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 |
Bud Site | 0.0042 | 0.0109 | 0.0009 | 0.0002 | 0.0004 | 0.0004 | 0.0026 | 0.0006 | 0.0037 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Cell Periphery | 0.0002 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 |
Cytoplasm | 0.0082 | 0.0035 | 0.0102 | 0.0019 | 0.0004 | 0.0045 | 0.0017 | 0.0013 | 0.001 | 0.0002 | 0.001 | 0.0011 | 0.0003 | 0.0004 | 0.0007 | 0.0009 | 0.0019 | 0.0001 |
Cytoplasmic Foci | 0.0951 | 0.1036 | 0.0691 | 0.0964 | 0.0698 | 0.0746 | 0.0638 | 0.0757 | 0.0697 | 0.0413 | 0.0583 | 0.0473 | 0.0564 | 0.0574 | 0.0567 | 0.0638 | 0.045 | 0.043 |
Eisosomes | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0015 | 0.0014 | 0.0007 | 0.0005 | 0.0003 | 0.0008 | 0.0017 | 0.0008 | 0.001 | 0.0002 | 0.0004 | 0.0006 | 0.0002 | 0.0003 | 0.0005 | 0.0002 | 0.0002 | 0.0001 |
Endosome | 0.0827 | 0.1351 | 0.0781 | 0.0559 | 0.1033 | 0.06 | 0.0822 | 0.0854 | 0.0889 | 0.0703 | 0.0739 | 0.0272 | 0.054 | 0.085 | 0.0461 | 0.0659 | 0.0509 | 0.024 |
Golgi | 0.0451 | 0.0568 | 0.0576 | 0.0467 | 0.1107 | 0.0568 | 0.0477 | 0.0541 | 0.0673 | 0.0863 | 0.0492 | 0.0311 | 0.0331 | 0.0406 | 0.04 | 0.0357 | 0.0364 | 0.0254 |
Lipid Particles | 0.569 | 0.5101 | 0.5318 | 0.4785 | 0.5566 | 0.6232 | 0.5885 | 0.6746 | 0.5553 | 0.6462 | 0.6276 | 0.758 | 0.7375 | 0.6943 | 0.6774 | 0.6603 | 0.7474 | 0.7892 |
Mitochondria | 0.0188 | 0.0298 | 0.0169 | 0.0272 | 0.0681 | 0.0116 | 0.0169 | 0.0039 | 0.0132 | 0.0228 | 0.0195 | 0.0034 | 0.0029 | 0.0081 | 0.0095 | 0.0043 | 0.0038 | 0.0069 |
None | 0.0021 | 0.0016 | 0.0181 | 0.001 | 0.0001 | 0.0017 | 0.006 | 0.0046 | 0.0021 | 0.0003 | 0.0031 | 0.001 | 0.003 | 0.0016 | 0.0029 | 0.0044 | 0.0116 | 0.0001 |
Nuclear Periphery | 0.0173 | 0.0048 | 0.002 | 0.0048 | 0.0004 | 0.0016 | 0.0051 | 0.0049 | 0.0014 | 0.0041 | 0.003 | 0.0064 | 0.0023 | 0.0009 | 0.0027 | 0.0021 | 0.0042 | 0.0017 |
Nucleolus | 0.0034 | 0.0146 | 0.0051 | 0.0007 | 0.0002 | 0.0008 | 0.0011 | 0.0012 | 0.0002 | 0.0002 | 0.0007 | 0.0009 | 0.0025 | 0.0024 | 0.0007 | 0.0034 | 0.0009 | 0.0016 |
Nucleus | 0.0024 | 0.0022 | 0.0031 | 0.0014 | 0.0001 | 0.0003 | 0.0019 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0.0004 | 0.0005 | 0.0007 | 0.0002 | 0.0025 | 0.0003 | 0.0003 |
Peroxisomes | 0.1111 | 0.0958 | 0.1771 | 0.255 | 0.0833 | 0.1454 | 0.1381 | 0.0815 | 0.1601 | 0.1233 | 0.1521 | 0.1079 | 0.0919 | 0.1011 | 0.1567 | 0.1485 | 0.0887 | 0.0976 |
Punctate Nuclear | 0.0169 | 0.0076 | 0.01 | 0.023 | 0.0009 | 0.0068 | 0.0143 | 0.0038 | 0.0016 | 0.0011 | 0.0054 | 0.0082 | 0.0131 | 0.0021 | 0.0032 | 0.0039 | 0.0053 | 0.0066 |
Vacuole | 0.0013 | 0.0138 | 0.0024 | 0.0009 | 0.0014 | 0.0016 | 0.0023 | 0.0023 | 0.0033 | 0.001 | 0.0019 | 0.0008 | 0.0008 | 0.0023 | 0.0011 | 0.0026 | 0.0017 | 0.0011 |
Vacuole Periphery | 0.0016 | 0.0029 | 0.0015 | 0.0012 | 0.0016 | 0.0013 | 0.0018 | 0.003 | 0.0019 | 0.002 | 0.0016 | 0.0012 | 0.001 | 0.0013 | 0.0012 | 0.001 | 0.0012 | 0.0011 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 39.7373 | 24.7833 | 14.0992 | 22.209 | 21.567 | 16.625 | 16.4968 | 16.9127 | 14.2724 | 31.3947 |
Translational Efficiency | 0.9205 | 0.633 | 1.0255 | 0.7545 | 1.0269 | 1.7108 | 1.1035 | 0.7967 | 0.9947 | 0.743 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1571 | 1378 | 1079 | 1120 | 1028 | 1771 | 2360 | 440 | 2599 | 3149 | 3439 | 1560 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 688.47 | 689.58 | 827.95 | 931.54 | 642.14 | 732.46 | 921.84 | 1028.55 | 670.14 | 713.70 | 892.38 | 958.90 |
Standard Deviation | 81.47 | 110.14 | 109.02 | 147.89 | 93.72 | 98.64 | 114.15 | 168.03 | 89.44 | 105.98 | 120.70 | 159.91 |
Intensity Change Log 2 | — | 0.002324 | 0.266150 | 0.436224 | — | 0.189862 | 0.521629 | 0.679652 | — | 0.095874 | 0.395094 | 0.558832 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001063 | 0.004982 | 0.006273 | 0.026476 | 0.002457 | 0.001044 | 0.038550 | 0.013939 | 0.001614 | 0.002767 | 0.028423 | 0.022939 |
Bud Neck | 0.003041 | 0.000828 | 0.010180 | 0.005160 | 0.001281 | 0.001355 | 0.000564 | 0.002090 | 0.002345 | 0.001125 | 0.003581 | 0.004294 |
Bud Site | 0.000705 | 0.000893 | 0.010723 | 0.015026 | 0.000426 | 0.000987 | 0.002255 | 0.009567 | 0.000595 | 0.000946 | 0.004912 | 0.013486 |
Cell Periphery | 0.000073 | 0.000053 | 0.000274 | 0.000153 | 0.000074 | 0.000055 | 0.000054 | 0.000126 | 0.000074 | 0.000054 | 0.000123 | 0.000145 |
Cytoplasm | 0.023924 | 0.004281 | 0.212946* | 0.004867 | 0.012513 | 0.007009 | 0.001235 | 0.004701 | 0.019410 | 0.005816 | 0.067660 | 0.004820 |
Cytoplasmic Foci | 0.239698* | 0.223071* | 0.102836 | 0.249546* | 0.239633* | 0.211054* | 0.368117* | 0.281640* | 0.239672* | 0.216312* | 0.284884* | 0.258598* |
Eisosomes | 0.000216 | 0.000091 | 0.000155 | 0.000100 | 0.000140 | 0.000090 | 0.000082 | 0.000070 | 0.000186 | 0.000090 | 0.000105 | 0.000091 |
Endoplasmic Reticulum | 0.001425 | 0.000727 | 0.016046 | 0.002731 | 0.001110 | 0.000494 | 0.000820 | 0.001583 | 0.001300 | 0.000596 | 0.005597 | 0.002407 |
Endosome | 0.097019 | 0.162154 | 0.057027 | 0.203574* | 0.124339 | 0.197387 | 0.184474 | 0.305039* | 0.107825 | 0.181969 | 0.144487 | 0.232192* |
Golgi | 0.140300* | 0.196625* | 0.003286 | 0.096463 | 0.178157* | 0.181166* | 0.078422 | 0.134135* | 0.155274* | 0.187931* | 0.054848 | 0.107089 |
Lipid Particles | 0.373484* | 0.294818* | 0.080266 | 0.081246 | 0.314163* | 0.283706* | 0.168687* | 0.076496 | 0.350020* | 0.288568* | 0.140945* | 0.079907 |
Mitochondria | 0.017301 | 0.012372 | 0.002047 | 0.017063 | 0.018121 | 0.025739 | 0.004140 | 0.008462 | 0.017625 | 0.019890 | 0.003483 | 0.014637 |
Mitotic Spindle | 0.012490 | 0.019600 | 0.058528 | 0.141746* | 0.010037 | 0.023222 | 0.057140 | 0.058050 | 0.011520 | 0.021637 | 0.057576 | 0.118140* |
None | 0.001191 | 0.000428 | 0.005254 | 0.000317 | 0.005313 | 0.000784 | 0.000018 | 0.000413 | 0.002821 | 0.000628 | 0.001661 | 0.000344 |
Nuclear Periphery | 0.000763 | 0.000306 | 0.015454 | 0.002055 | 0.000449 | 0.000571 | 0.000234 | 0.001361 | 0.000639 | 0.000455 | 0.005010 | 0.001859 |
Nuclear Periphery Foci | 0.005259 | 0.006596 | 0.102507 | 0.015957 | 0.003500 | 0.004904 | 0.003700 | 0.016643 | 0.004563 | 0.005645 | 0.034701 | 0.016151 |
Nucleolus | 0.002263 | 0.002044 | 0.011745 | 0.002725 | 0.004409 | 0.001898 | 0.000091 | 0.000947 | 0.003112 | 0.001962 | 0.003748 | 0.002224 |
Nucleus | 0.004640 | 0.001960 | 0.216283* | 0.005171 | 0.003196 | 0.001806 | 0.000127 | 0.000560 | 0.004069 | 0.001874 | 0.067947 | 0.003871 |
Peroxisomes | 0.068162 | 0.064799 | 0.018448 | 0.085722 | 0.075991 | 0.047781 | 0.086248 | 0.038190 | 0.071259 | 0.055228 | 0.064976 | 0.072315 |
Vacuole | 0.005398 | 0.002362 | 0.069127 | 0.037981 | 0.003654 | 0.006645 | 0.004465 | 0.039597 | 0.004708 | 0.004771 | 0.024753 | 0.038437 |
Vacuole Periphery | 0.001586 | 0.001011 | 0.000594 | 0.005922 | 0.001038 | 0.002303 | 0.000577 | 0.006389 | 0.001369 | 0.001738 | 0.000582 | 0.006054 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.50 | -3.04 | -8.35 | -6.77 | -6.42 | 2.33 | -13.72 | -3.62 | -4.08 | 6.09 | -2.50 | -14.27 | -9.00 | -8.40 | 1.83 |
Bud Neck | 2.97 | -6.34 | -2.33 | -5.64 | 4.59 | -0.30 | 3.38 | -2.37 | -2.30 | -4.74 | 2.68 | -3.28 | -3.09 | -5.81 | -0.29 |
Bud Site | -0.64 | -6.17 | -10.45 | -10.20 | -2.43 | -3.22 | -6.00 | -5.07 | -4.76 | -3.99 | -2.13 | -7.82 | -11.63 | -11.27 | -7.17 |
Cell Periphery | 2.87 | -13.68 | -7.25 | -9.69 | 6.86 | 2.01 | 2.45 | -2.18 | -3.17 | -3.30 | 3.35 | -7.06 | -7.01 | -9.41 | -2.54 |
Cytoplasm | 8.69 | -23.07 | 7.53 | -1.64 | 26.25 | 2.97 | 6.57 | 3.29 | 0.96 | -2.65 | 8.73 | -13.77 | 8.03 | -0.36 | 19.62 |
Cytoplasmic Foci | 2.69 | 20.21 | -0.16 | -2.52 | -17.81 | 4.49 | -16.39 | -2.93 | -6.11 | 8.13 | 5.23 | -7.45 | -1.54 | -5.81 | 4.70 |
Eisosomes | 5.93 | 2.63 | 5.46 | -1.53 | 6.28 | 5.20 | 6.35 | 5.80 | 2.17 | 1.27 | 7.22 | 6.07 | 6.91 | -0.47 | 2.44 |
Endoplasmic Reticulum | 2.18 | -12.05 | -3.63 | -6.26 | 10.55 | 1.09 | 0.46 | -0.83 | -3.60 | -2.47 | 2.43 | -9.05 | -3.55 | -7.48 | 6.28 |
Endosome | -10.96 | 9.19 | -15.18 | -5.07 | -22.33 | -10.21 | -9.90 | -15.09 | -8.91 | -10.40 | -16.06 | -8.98 | -20.13 | -7.47 | -14.21 |
Golgi | -8.46 | 30.23 | 7.51 | 15.81 | -22.79* | -0.63 | 15.04 | 4.37 | 5.31 | -6.69 | -6.76 | 25.06 | 9.45 | 16.49 | -12.79 |
Lipid Particles | 6.58 | 32.64* | 30.70 | 22.36 | -1.46 | 2.43 | 13.62 | 19.86 | 19.92 | 10.56 | 7.19 | 29.06 | 36.46 | 30.81 | 11.06 |
Mitochondria | 2.44 | 10.29 | 0.15 | -1.71 | -6.59 | -2.96 | 7.15 | 3.55 | 6.20 | -1.96 | -1.42 | 11.37 | 1.52 | 2.61 | -6.21 |
Mitotic Spindle | -2.52 | -8.56 | -16.66 | -15.56 | -9.28 | -5.20 | -13.35 | -7.10 | -5.09 | -0.51 | -5.11 | -15.24 | -18.02 | -16.09 | -9.55 |
None | 2.94 | -5.42 | 2.77 | 0.38 | 6.82 | 3.01 | 3.81 | 3.48 | 1.24 | -1.28 | 3.42 | 2.01 | 4.18 | 1.39 | 4.77 |
Nuclear Periphery | 3.26 | -11.97 | -2.14 | -2.94 | 10.04 | -0.66 | 1.34 | -2.93 | -2.71 | -4.15 | 1.53 | -10.65 | -2.76 | -3.21 | 5.70 |
Nuclear Periphery Foci | -0.71 | -17.68 | -5.14 | -4.18 | 15.12 | -1.29 | 0.20 | -4.02 | -3.60 | -4.07 | -0.97 | -15.21 | -6.66 | -5.96 | 7.72 |
Nucleolus | 0.08 | -7.94 | -0.83 | -0.86 | 7.16 | 2.58 | 4.87 | 3.59 | 1.96 | -1.59 | 1.78 | -2.21 | 1.20 | -0.31 | 3.34 |
Nucleus | 3.10 | -25.00 | -1.19 | -2.67 | 24.27 | 1.96 | 5.23 | 4.37 | 2.16 | -1.95 | 3.92 | -19.97 | -0.65 | -2.47 | 18.45 |
Peroxisomes | 0.60 | 13.52 | -2.73 | -3.09 | -12.06 | 5.39 | -1.90 | 5.85 | 1.92 | 8.26 | 4.56 | 1.71 | -0.13 | -3.51 | -1.44 |
Vacuole | 4.79 | -19.77 | -11.90 | -12.95 | 8.17 | -2.97 | -1.27 | -7.51 | -6.95 | -7.32 | -0.09 | -16.64 | -14.15 | -14.11 | -4.46 |
Vacuole Periphery | 2.08 | 4.74 | -5.05 | -5.79 | -6.46 | -4.73 | 2.65 | -5.25 | -3.95 | -5.74 | -1.86 | 5.18 | -6.94 | -6.37 | -8.28 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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