Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Cytoplasm 0.92 0.94 – 1.0 0.99 0.98 0.96 0.94 0.87 0.92 0.56 0.75 0.99 1.0 1.0 0.98 0.99 0.97 0.84 0.84 0.75 0.78 0.74 0.77
Endoplasmic Reticulum 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0.05 0 0 0
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0.06 – 0 0 0 0.09 0.09 0.33 0.19 0.66 0.4 0 0 0 0 0 0 0.07 0.07 0.11 0.06 0.07 0.08
Nucleus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0.06 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 2 0 1 0 0 0 0 0 0 0 0 1 0 0 0 1 2
Bud 1 0 – 0 0 1 4 4 2 6 4 1 0 0 0 0 0 0 0 0 0 0 3 4
Bud Neck 0 0 – 2 6 0 8 12 4 6 0 1 1 1 0 1 0 0 0 0 0 0 1 1
Bud Site 0 0 – 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 1 0 3 0 4 2 1 1 4 2 2 1 6 5 7 0 0 0 1 2 0
Cytoplasm 49 15 – 120 213 295 503 376 169 174 79 110 175 342 365 222 72 249 50 31 16 104 165 211
Endoplasmic Reticulum 0 0 – 0 0 0 0 1 0 0 0 0 1 0 0 9 6 10 0 0 0 1 3 3
Endosome 0 0 – 0 0 1 1 0 0 0 0 0 0 0 0 0 0 3 0 0 0 1 2 1
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 7 1 – 1 0 10 47 36 64 37 93 59 0 0 0 2 0 3 4 2 2 7 16 21
Nucleus 0 0 – 0 0 0 1 4 3 0 3 1 0 1 0 1 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1 0 0
SpindlePole 0 0 – 0 1 2 5 5 2 1 2 6 0 1 1 0 0 0 0 0 0 0 3 2
Vac/Vac Membrane 0 0 – 0 0 0 1 0 0 0 0 1 0 1 0 3 3 2 1 1 0 8 13 14
Unique Cell Count 53 16 120 216 300 523 402 194 190 141 147 176 343 366 226 73 257 60 37 22 134 223 274
Labelled Cell Count 57 16 124 221 312 571 444 246 226 183 184 179 348 367 244 86 277 60 37 22 134 223 274


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 2.9 3.4 – 5.1 4.8 4.0 3.7 4.1 3.6 3.8 3.6 3.8 5.3 5.3 5.3 6.6 7.0 7.0 4.7 5.0 5.3
Std Deviation (1e-4) 0.2 0.6 – 2.2 1.5 1.5 1.5 1.2 1.7 1.7 1.7 1.1 1.5 1.4 1.5 1.3 1.6 1.9 1.8 1.8 2.1
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.819 1.3911 2.033 0.545 3.5478 1.3948 1.106 1.7997 1.7407 1.3536 0.183 1.6983 -0.0948 1.3067 0.9397 0.6995 0.2096 0.4831
Actin 0.001 0.0025 0.0064 0.0082 0.2167 0.0149 0.009 0.0005 0.0011 0.0001 0.0004 0.0003 0.0274 0.0209 0.0725 0.0369 0.0001 0.0064
Bud 0.0025 0.0533 0.0342 0.0407 0.0079 0.0069 0.0005 0.0003 0.0013 0.0002 0.0005 0.0001 0.0005 0.0007 0.0041 0.0046 0.0001 0.0002
Bud Neck 0.0003 0.0007 0.0037 0.019 0.0026 0.001 0.0084 0.0002 0.0005 0.0003 0.0003 0.0006 0.0012 0.0004 0.0042 0.0021 0.0002 0.0007
Bud Periphery 0.0033 0.0099 0.0096 0.0723 0.0123 0.0237 0.0006 0.0004 0.0015 0.0001 0.0008 0.0001 0.0005 0.0004 0.0054 0.0049 0.0001 0.0001
Bud Site 0.0022 0.0543 0.0552 0.0096 0.0648 0.0045 0.0194 0.0016 0.0245 0.0001 0.0013 0.0002 0.0035 0.0172 0.1049 0.0207 0.0002 0.0001
Cell Periphery 0.0011 0.0023 0.0011 0.0024 0.0032 0.0016 0.0022 0.0007 0.0009 0.0001 0.0012 0.0001 0.0003 0.0011 0.0012 0.0007 0.0001 0.0001
Cytoplasm 0.408 0.4129 0.528 0.1595 0.0025 0.5073 0.4629 0.7241 0.62 0.6632 0.5731 0.8496 0.3619 0.7372 0.4427 0.594 0.6484 0.8092
Cytoplasmic Foci 0.0182 0.0256 0.0195 0.1454 0.1566 0.0201 0.0654 0.0212 0.0484 0.0371 0.0303 0.0196 0.0583 0.0258 0.0342 0.0401 0.038 0.013
Eisosomes 0.0005 0.0002 0.0002 0.0002 0.0047 0.0002 0.0005 0.0001 0.0003 0 0.0002 0 0.0003 0.0003 0.0006 0.0002 0 0.0001
Endoplasmic Reticulum 0.0157 0.0034 0.0024 0.0057 0.0005 0.0164 0.02 0.0092 0.0073 0.0018 0.0347 0.0075 0.0208 0.0051 0.0266 0.0074 0.0119 0.0105
Endosome 0.0691 0.0247 0.019 0.1188 0.0371 0.0551 0.0584 0.0229 0.0491 0.0551 0.1098 0.0355 0.0589 0.0243 0.0544 0.1655 0.1751 0.0906
Golgi 0.0026 0.0028 0.0019 0.0435 0.0491 0.0048 0.0069 0.0021 0.0029 0.0005 0.0048 0.008 0.0094 0.0038 0.0137 0.0437 0.0028 0.0049
Lipid Particles 0.0123 0.001 0.0009 0.0029 0.0289 0.0091 0.0395 0.0015 0.0037 0.0002 0.006 0.0034 0.0302 0.0021 0.0052 0.0148 0.002 0.0023
Mitochondria 0.0085 0.0029 0.0027 0.0058 0.1822 0.0052 0.0071 0.0031 0.0011 0.0003 0.0053 0.0015 0.0043 0.0017 0.0202 0.0309 0.0009 0.0016
None 0.3454 0.3764 0.2974 0.312 0.0011 0.2801 0.2238 0.2006 0.2169 0.2272 0.1741 0.0609 0.1621 0.1357 0.1875 0.0009 0.0699 0.0183
Nuclear Periphery 0.0188 0.0013 0.0007 0.0044 0.0003 0.0134 0.0118 0.0014 0.0019 0.0009 0.0069 0.0012 0.035 0.0012 0.0017 0.0014 0.0049 0.0255
Nucleolus 0.002 0.0024 0.0013 0.0019 0.0004 0.001 0.0031 0.0002 0.0008 0.0001 0.0004 0.0001 0.0061 0.0003 0.0004 0.0004 0.0002 0.0003
Nucleus 0.0184 0.0086 0.0033 0.0088 0.0004 0.0047 0.0115 0.0009 0.0028 0.0013 0.0025 0.0033 0.0967 0.002 0.0013 0.0011 0.007 0.0035
Peroxisomes 0.0011 0.0007 0.0011 0.009 0.2238 0.001 0.0103 0.0002 0.0009 0.0001 0.0003 0.0007 0.0357 0.002 0.0093 0.0109 0.0002 0.0003
Punctate Nuclear 0.0042 0.0046 0.0009 0.0043 0.0024 0.0113 0.025 0.0005 0.0019 0.0004 0.0006 0.0011 0.075 0.0113 0.0013 0.0007 0.0011 0.0057
Vacuole 0.0514 0.0083 0.0097 0.0215 0.0015 0.0147 0.0112 0.0066 0.011 0.0091 0.0406 0.0054 0.0087 0.0061 0.0065 0.0147 0.0314 0.0046
Vacuole Periphery 0.0134 0.0011 0.001 0.0041 0.0008 0.0029 0.0026 0.0018 0.0013 0.0019 0.0058 0.0008 0.0033 0.0006 0.0019 0.0034 0.0054 0.0021

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1757.0134 1815.6861 1962.7011 1929.269 1971.268 1430.5879 1677.5581 1509.1752 1537.5083 1642.1759
Translational Efficiency 1.4371 1.3845 1.3219 1.0782 1.5362 1.9087 1.2698 1.4625 1.4035 1.5239

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
351 467 2360 1045 1245 509 1561 1131 1596 976 3921 2176

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 496.67 576.76 653.05 565.12 508.41 565.33 765.28 823.31 505.83 570.80 697.73 699.32
Standard Deviation 76.56 116.61 137.80 183.57 94.37 112.07 96.00 110.31 90.88 114.41 134.60 197.86
Intensity Change Log 2 — 0.215684 0.394906 0.186270 — 0.153101 0.589995 0.695443 — 0.184366 0.496883 0.466144

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000067 0.000118 0.000086 0.000372 0.000050 0.000054 0.000174 0.000186 0.000054 0.000084 0.000121 0.000275
Bud Neck 0.039716 0.040875 0.008052 0.011943 0.015616 0.021253 0.003762 0.002571 0.020916 0.030641 0.006344 0.007072
Bud Site 0.001976 0.010229 0.008083 0.013193 0.002499 0.003308 0.013152 0.011176 0.002384 0.006620 0.010101 0.012145
Cell Periphery 0.001005 0.000435 0.000114 0.005689 0.001831 0.000736 0.000134 0.000079 0.001649 0.000592 0.000122 0.002773
Cytoplasm 0.410588* 0.394815* 0.718983* 0.426602* 0.464789* 0.490330* 0.624115* 0.685668* 0.452869* 0.444628* 0.681215* 0.561254*
Cytoplasmic Foci 0.304804* 0.333785* 0.061225 0.098634 0.227514* 0.229310* 0.091584 0.137165 0.244512* 0.279299* 0.073312 0.118661
Eisosomes 0.004332 0.000574 0.000070 0.010004 0.007180 0.003174 0.000051 0.000029 0.006554 0.001930 0.000063 0.004820
Endoplasmic Reticulum 0.001952 0.001595 0.000871 0.005716 0.002135 0.000783 0.001185 0.000882 0.002095 0.001171 0.000996 0.003203
Endosome 0.016409 0.037052 0.004706 0.012969 0.009637 0.011426 0.009660 0.008771 0.011127 0.023687 0.006678 0.010787
Golgi 0.013541 0.004755 0.000361 0.004466 0.005095 0.004803 0.000753 0.001673 0.006952 0.004780 0.000517 0.003014
Lipid Particles 0.058029 0.020632 0.002320 0.018595 0.024208 0.017289 0.002033 0.001579 0.031647 0.018888 0.002206 0.009751
Mitochondria 0.021441 0.004910 0.000609 0.001789 0.005285 0.005321 0.001181 0.000267 0.008838 0.005125 0.000836 0.000998
Mitotic Spindle 0.000072 0.000152 0.001754 0.005308 0.000989 0.000263 0.003693 0.004934 0.000787 0.000210 0.002526 0.005114
None 0.010832 0.020693 0.012346 0.105233* 0.038228 0.042754 0.002759 0.002217 0.032203 0.032198 0.008529 0.051689
Nuclear Periphery 0.000581 0.000818 0.000552 0.000895 0.000657 0.000492 0.000567 0.000225 0.000640 0.000648 0.000558 0.000547
Nuclear Periphery Foci 0.000316 0.000526 0.000901 0.003426 0.000687 0.000393 0.001576 0.003361 0.000606 0.000457 0.001170 0.003392
Nucleolus 0.001007 0.002332 0.000790 0.003273 0.002095 0.001066 0.000378 0.000333 0.001856 0.001672 0.000626 0.001745
Nucleus 0.075482 0.066542 0.108395 0.185282* 0.146356* 0.118618 0.092881 0.033575 0.130769* 0.093700 0.102218 0.106431
Peroxisomes 0.012308 0.008206 0.000562 0.015649 0.010646 0.009104 0.000828 0.000812 0.011011 0.008674 0.000668 0.007937
Vacuole 0.023590 0.049230 0.068292 0.070198 0.033487 0.038776 0.148635 0.103992 0.031311 0.043778 0.100277 0.087763
Vacuole Periphery 0.001951 0.001728 0.000929 0.000764 0.001014 0.000749 0.000898 0.000504 0.001220 0.001217 0.000916 0.000629

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.70 -1.02 -3.32 -2.79 -3.06 -0.36 -4.12 -3.46 -3.34 -0.27 -3.12 -4.11 -4.51 -3.89 -3.05
Bud Neck -0.17 6.65 5.78 5.90 -2.12 -1.90 9.63 10.83 6.76 2.66 -3.16 9.86 9.11 8.48 -0.54
Bud Site -4.52 -6.38 -5.77 -1.18 -2.68 -1.11 -5.45 -4.98 -4.27 0.55 -4.37 -7.92 -7.47 -3.41 -1.71
Cell Periphery 3.62 6.44 -8.14 -9.56 -10.35 4.36 7.83 8.06 5.35 3.20 5.63 8.88 -2.80 -7.59 -9.99
Cytoplasm 0.89 -21.95 -0.77 -1.83 27.43 -1.90 -11.29 -18.86 -12.32 -7.95 0.79 -27.20 -10.50 -9.59 14.05
Cytoplasmic Foci -1.75 20.66 16.29 19.69 -7.75 -0.19 22.65 13.41 9.83 -8.65 -3.93 32.36 21.10 20.66 -12.53
Eisosomes 3.22 3.69 -2.84 -7.45 -7.99 2.62 5.69 5.70 3.61 8.05 4.16 6.43 1.98 -3.31 -7.84
Endoplasmic Reticulum 0.52 2.08 -4.03 -4.55 -6.54 3.75 2.69 4.07 -0.44 3.77 2.59 3.96 -2.22 -4.46 -5.84
Endosome -4.96 7.41 1.63 5.94 -8.58 -1.17 1.00 1.04 1.92 -0.02 -5.89 6.52 0.26 6.11 -7.35
Golgi 4.63 7.61 5.32 0.79 -8.36 0.28 9.22 6.32 3.09 -1.91 2.64 12.38 7.20 2.93 -7.37
Lipid Particles 6.41 10.68 7.58 1.27 -9.58 2.52 13.39 13.59 7.27 2.07 5.22 16.67 11.89 5.46 -8.93
Mitochondria 4.40 5.63 5.32 4.88 -3.96 -0.01 4.28 5.38 2.50 1.94 2.39 7.26 7.10 3.80 -0.91
Mitotic Spindle -2.07 -5.65 -4.69 -4.64 -3.58 1.60 -2.63 -3.13 -3.88 -1.19 1.67 -3.17 -5.17 -6.05 -3.64
None -1.83 -0.14 -10.57 -8.34 -11.04 -0.49 8.20 8.33 5.18 0.47 -0.00 6.92 -2.76 -2.33 -10.21
Nuclear Periphery -0.75 -0.11 -3.30 -0.38 -3.86 1.26 -1.36 3.88 1.46 7.48 -0.04 -0.34 0.31 0.18 0.87
Nuclear Periphery Foci -2.48 -5.75 -8.72 -8.20 -7.03 2.54 -5.16 -8.20 -9.25 -5.65 1.75 -5.11 -11.34 -12.36 -9.29
Nucleolus -1.40 1.04 -2.35 -1.11 -5.46 2.47 4.17 3.96 3.95 -0.91 0.67 3.85 0.65 -0.04 -5.25
Nucleus 1.22 -6.40 -11.11 -11.95 -8.26 3.97 7.98 23.71 14.29 19.03 7.06 5.58 3.86 -2.44 -0.17
Peroxisomes 2.36 6.67 -0.34 -2.20 -5.30 0.53 5.60 5.55 3.79 -0.59 1.32 7.34 2.07 0.84 -5.32
Vacuole -5.26 -13.77 -11.25 -6.28 -1.42 -1.37 -27.12 -17.93 -14.87 8.48 -4.21 -28.60 -20.34 -15.10 3.47
Vacuole Periphery 0.70 2.83 4.39 5.26 0.53 1.55 1.08 3.81 2.90 1.48 0.02 1.81 4.79 5.93 1.30
Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (13%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rps11b

Rps11b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rps11b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available