Standard name
Description Transcriptional repressor; mediates glucose repression and negatively regulates filamentous growth; activated in stochastic pulses of nuclear localization in response to low glucose

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.29 0.32 0.81 0.64 0.59 0.54 0.46 0.41 0.43 0.41 0.41 0.64 0.78 0.73 0.89 0.83 0.77 0.18 0.22 0.46 0.24 0.33 0.27
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.08 0 0 0 0 0 0
Endosome 0.13 0 0 0.11 0.08 0 0.08 0 0 0 0 0.1 0 0.09 0 0 0 0.06 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0.19 0 0.06 0.06 0.49 0.24 0.47 0.36 0.48 0.46 0 0 0 0 0 0 0 0.05 0.08 0.05 0.06 0.07
Nucleus 0.29 0.29 0 0.09 0.09 0.06 0.15 0.15 0.16 0.11 0.12 0.09 0.05 0 0 0 0 0.35 0.34 0.12 0.27 0.14 0.16
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0.09 0.07 0.06 0.09 0.1 0.1
Vac/Vac Membrane 0.3 0.27 0.1 0.19 0.25 0 0.14 0.11 0.11 0.1 0.1 0.27 0.17 0.31 0.16 0.26 0.22 0.2 0.23 0.18 0.2 0.19 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 3 0 6 2
Bud 0 0 0 0 0 4 0 4 4 6 8 0 0 0 0 0 2 6 3 3 4 12 18
Bud Neck 0 0 5 0 3 2 6 5 2 4 2 1 1 0 0 0 0 2 1 1 2 6 12
Bud Site 1 0 0 0 0 3 0 3 1 5 1 0 0 0 0 0 0
Cell Periphery 0 0 1 0 0 0 0 0 0 0 1 1 0 1 1 0 1 0 0 0 0 0 0
Cytoplasm 86 84 155 141 157 157 151 189 159 188 185 192 301 249 92 72 80 72 82 131 53 130 87
Endoplasmic Reticulum 1 2 2 0 0 0 0 1 3 1 0 1 0 0 7 1 8 0 0 2 2 4 3
Endosome 38 9 7 25 22 2 27 6 8 12 10 31 14 32 4 0 3 23 9 4 7 16 12
Golgi 1 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 5 1 1 2 3 1
Mitochondria 33 49 1 14 15 144 78 217 133 219 210 4 6 1 1 1 3 12 17 22 10 22 22
Nucleus 88 76 7 19 24 17 51 70 59 52 57 27 18 12 1 0 1 139 125 34 60 54 52
Nuclear Periphery 0 0 0 0 0 1 2 0 0 0 2 0 0 0 1 0 0 1 0 0 0 2 1
Nucleolus 1 0 0 0 0 2 0 0 1 1 0 0 0 0 1 0 0 7 6 1 4 5 8
Peroxisomes 0 0 0 0 0 0 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 3 5 2 5 7 3 20 6 7 8 13 0 2 1 0 0 0 37 24 15 21 41 30
Vac/Vac Membrane 90 69 19 42 67 12 46 52 41 44 46 81 64 105 16 23 23 79 85 50 45 75 53
Unique Cell Count 299 259 191 222 265 291 330 461 366 456 456 299 384 340 103 87 104 404 371 287 225 397 321
Labelled Cell Count 342 294 199 246 295 347 382 553 419 540 535 339 408 402 124 97 121 404 371 287 225 397 321


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 4.0 4.9 4.8 5.1 3.6 4.3 3.6 3.9 3.7 3.9 5.1 4.6 5.6 5.7 6.0 5.5 4.4 4.7 5.1
Std Deviation (1e-4) 0.8 0.6 0.7 1.3 1.3 1.5 1.2 0.6 0.8 0.7 0.7 1.3 1.3 2.3 1.5 0.9 1.3 1.1 1.6 2.0
Intensity Change (Log2) -0.02 0.06 -0.45 -0.21 -0.46 -0.32 -0.4 -0.34 0.05 -0.1 0.2 0.22 0.29 0.17 -0.16 -0.05 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 1.4 3.6 2.9


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nrg2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available