Standard name | |
---|---|
Human Ortholog | |
Description | Component of UDP-GlcNAc transferase; required for second step of dolichyl-linked oligosaccharide synthesis; anchors catalytic subunit Alg13p to ER membrane; similar to bacterial and human glycosyltransferases; both human homologs ALG13 and ALG14 are required to complement yeast alg14 mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 5 | 6 | 23 | 9 | 5 | 1 | 4 | 0 | 5 | 11 | 12 |
Bud | 0 | 0 | 0 | 2 | 1 | 1 | 5 | 11 | 1 | 8 | 5 | 14 | 4 | 4 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 3 | 5 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 3 | 0 | 0 | 0 | 0 | 1 | 1 | 5 | 10 | 9 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 3 | 1 | 2 | 0 | 4 | 2 | 3 | 0 | 4 | 14 | 10 | 17 | 14 | 14 | 9 | 0 | 1 | 2 | 7 | 14 | 13 |
Cytoplasm | 12 | 23 | 24 | 86 | 185 | 73 | 127 | 198 | 86 | 147 | 116 | 146 | 175 | 311 | 254 | 83 | 58 | 44 | 10 | 24 | 23 | 73 | 167 | 187 |
Endoplasmic Reticulum | 0 | 19 | 5 | 11 | 12 | 7 | 10 | 2 | 6 | 10 | 10 | 4 | 39 | 70 | 72 | 4 | 3 | 2 | 15 | 35 | 28 | 50 | 95 | 47 |
Endosome | 0 | 1 | 0 | 1 | 4 | 0 | 0 | 4 | 1 | 3 | 0 | 0 | 0 | 2 | 1 | 5 | 3 | 0 | 2 | 2 | 1 | 2 | 7 | 4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 2 | 0 | 0 | 1 | 2 |
Mitochondria | 5 | 1 | 4 | 14 | 25 | 31 | 80 | 89 | 90 | 131 | 123 | 187 | 0 | 0 | 1 | 14 | 12 | 4 | 5 | 10 | 11 | 29 | 73 | 53 |
Nucleus | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 3 | 5 | 5 | 3 | 4 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 4 | 1 | 4 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 2 | 4 | 3 |
Vac/Vac Membrane | 0 | 2 | 0 | 2 | 13 | 4 | 5 | 10 | 9 | 16 | 5 | 5 | 3 | 5 | 9 | 10 | 16 | 6 | 1 | 7 | 2 | 19 | 31 | 42 |
Unique Cell Count | 16 | 46 | 33 | 105 | 227 | 103 | 181 | 269 | 174 | 267 | 205 | 299 | 224 | 389 | 344 | 147 | 110 | 65 | 39 | 94 | 73 | 204 | 429 | 395 |
Labelled Cell Count | 18 | 47 | 33 | 121 | 243 | 119 | 227 | 324 | 209 | 329 | 264 | 370 | 238 | 408 | 369 | 160 | 117 | 72 | 39 | 94 | 73 | 204 | 429 | 395 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.0 | 6.3 | 4.5 | 4.4 | 4.7 | 4.1 | 3.9 | 3.8 | 3.7 | 3.5 | 3.4 | 3.4 | 6.0 | 6.0 | 6.3 | 6.9 | 7.0 | 7.1 | 6.2 | 6.2 | 6.2 |
Std Deviation (1e-4) | 2.4 | 1.1 | 1.1 | 1.1 | 2.2 | 1.7 | 2.6 | 1.9 | 1.6 | 1.0 | 1.2 | 1.0 | 0.9 | 1.0 | 1.2 | 1.0 | 1.2 | 1.1 | 0.9 | 1.3 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.06 | -0.13 | -0.19 | -0.22 | -0.25 | -0.35 | -0.39 | -0.4 | 0.43 | 0.44 | 0.51 | 0.62 | 0.65 | 0.66 | 0.48 | 0.48 | 0.47 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.1 | 1.2 | -0.2 | -0.3 | 0.1 | -2.5 | -1.9 | -1.7 | -2.6 | 0.7 | 1.0 | 0.1 | -1.7 | -2.0 | -0.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.3 | 0.4 | 0.8 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.3705 | 1.692 | 1.695 | 1.1701 | 1.4538 | 1.3257 | 0.9192 | 1.692 | 1.2359 | 0.6464 | 0.4293 | 1.2714 | -0.1989 | 0.043 | 0.1194 | -0.0833 | -0.42 | -0.022 |
Actin | 0.0189 | 0.0042 | 0.0068 | 0.0059 | 0.0065 | 0.0164 | 0.0255 | 0.0021 | 0.0037 | 0.004 | 0.0014 | 0.0122 | 0.0157 | 0.0019 | 0.0122 | 0.0072 | 0.0054 | 0.0006 |
Bud | 0.0004 | 0.0001 | 0.0016 | 0.0002 | 0.0019 | 0.0003 | 0.0002 | 0.0001 | 0.0011 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 |
Bud Neck | 0.0028 | 0.0004 | 0.001 | 0.0007 | 0.0013 | 0.0061 | 0.0028 | 0.0009 | 0.0009 | 0.0013 | 0.0029 | 0.0153 | 0.0014 | 0.0003 | 0.0016 | 0.0034 | 0.0003 | 0.0008 |
Bud Periphery | 0.0008 | 0.0002 | 0.0012 | 0.0007 | 0.0024 | 0.0006 | 0.0004 | 0.0002 | 0.0014 | 0.0003 | 0.0005 | 0.0003 | 0.0005 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0 |
Bud Site | 0.0056 | 0.003 | 0.0268 | 0.0004 | 0.0045 | 0.0009 | 0.0026 | 0.0187 | 0.0033 | 0.0003 | 0.0006 | 0.0005 | 0.0016 | 0.0021 | 0.0012 | 0.0003 | 0.0009 | 0.0001 |
Cell Periphery | 0.003 | 0.0027 | 0.0027 | 0.0012 | 0.0009 | 0.0008 | 0.0041 | 0.0037 | 0.0032 | 0.0007 | 0.002 | 0.0016 | 0.0017 | 0.002 | 0.0057 | 0.0024 | 0.0009 | 0.0005 |
Cytoplasm | 0.2935 | 0.49 | 0.3314 | 0.4356 | 0.4036 | 0.3752 | 0.3139 | 0.332 | 0.3629 | 0.2954 | 0.384 | 0.3923 | 0.2404 | 0.3837 | 0.3378 | 0.3522 | 0.379 | 0.4616 |
Cytoplasmic Foci | 0.05 | 0.0162 | 0.0299 | 0.0148 | 0.0468 | 0.0242 | 0.0171 | 0.0162 | 0.0284 | 0.0238 | 0.0413 | 0.0113 | 0.0181 | 0.026 | 0.014 | 0.0108 | 0.0273 | 0.0086 |
Eisosomes | 0.0016 | 0.0003 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0006 | 0.0003 | 0.0008 | 0.0009 | 0.0001 | 0.0003 | 0.0007 | 0.0002 | 0.0004 | 0.0004 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.2271 | 0.2178 | 0.3372 | 0.3156 | 0.1454 | 0.2691 | 0.2206 | 0.2118 | 0.3019 | 0.2652 | 0.3217 | 0.1539 | 0.4024 | 0.3715 | 0.4227 | 0.4978 | 0.3586 | 0.389 |
Endosome | 0.0407 | 0.021 | 0.029 | 0.0205 | 0.0849 | 0.0192 | 0.053 | 0.015 | 0.0421 | 0.0274 | 0.0162 | 0.0064 | 0.0259 | 0.0183 | 0.0164 | 0.0423 | 0.0327 | 0.0144 |
Golgi | 0.0128 | 0.0023 | 0.0125 | 0.0049 | 0.0372 | 0.0043 | 0.0132 | 0.0024 | 0.0082 | 0.0012 | 0.0017 | 0.0017 | 0.0067 | 0.0016 | 0.0086 | 0.0049 | 0.0073 | 0.0006 |
Lipid Particles | 0.0392 | 0.0119 | 0.0142 | 0.0074 | 0.0166 | 0.0118 | 0.0181 | 0.0111 | 0.0249 | 0.0059 | 0.0051 | 0.0055 | 0.0403 | 0.0156 | 0.0256 | 0.0143 | 0.0406 | 0.0033 |
Mitochondria | 0.0079 | 0.0004 | 0.0025 | 0.0463 | 0.0125 | 0.0032 | 0.0096 | 0.0006 | 0.0048 | 0.0009 | 0.0005 | 0.0008 | 0.0118 | 0.0003 | 0.0131 | 0.0011 | 0.0015 | 0.0003 |
None | 0.2487 | 0.2092 | 0.1692 | 0.1204 | 0.1928 | 0.2329 | 0.2721 | 0.3734 | 0.181 | 0.3493 | 0.2107 | 0.3818 | 0.1936 | 0.153 | 0.1152 | 0.0338 | 0.11 | 0.1045 |
Nuclear Periphery | 0.0065 | 0.0094 | 0.0084 | 0.0116 | 0.0085 | 0.0077 | 0.0235 | 0.0032 | 0.0107 | 0.0109 | 0.0033 | 0.0029 | 0.0115 | 0.0109 | 0.0089 | 0.0158 | 0.0077 | 0.0092 |
Nucleolus | 0.0012 | 0.0002 | 0.0004 | 0.0001 | 0.0018 | 0.0003 | 0.0006 | 0.0002 | 0.0005 | 0.0003 | 0.0001 | 0.0002 | 0.0015 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0 |
Nucleus | 0.0025 | 0.0026 | 0.0032 | 0.0037 | 0.0072 | 0.0087 | 0.0035 | 0.0014 | 0.0046 | 0.0029 | 0.0012 | 0.0019 | 0.0033 | 0.0024 | 0.0015 | 0.0023 | 0.0027 | 0.0019 |
Peroxisomes | 0.0146 | 0.0004 | 0.0084 | 0.0005 | 0.0076 | 0.0115 | 0.0032 | 0.0002 | 0.0029 | 0.0007 | 0.0009 | 0.0023 | 0.01 | 0.0003 | 0.0011 | 0.0001 | 0.0147 | 0.0001 |
Punctate Nuclear | 0.0143 | 0.0012 | 0.0042 | 0.0027 | 0.0039 | 0.0024 | 0.0014 | 0.0012 | 0.0032 | 0.0028 | 0.0013 | 0.0057 | 0.0052 | 0.004 | 0.0078 | 0.0007 | 0.0054 | 0.0007 |
Vacuole | 0.0069 | 0.0059 | 0.0086 | 0.0054 | 0.0107 | 0.0036 | 0.0103 | 0.0048 | 0.0086 | 0.0051 | 0.004 | 0.0028 | 0.0062 | 0.0051 | 0.0047 | 0.0088 | 0.0039 | 0.003 |
Vacuole Periphery | 0.001 | 0.0005 | 0.0007 | 0.001 | 0.0028 | 0.0006 | 0.0037 | 0.0004 | 0.0011 | 0.0005 | 0.0003 | 0.0002 | 0.0012 | 0.0004 | 0.0007 | 0.0011 | 0.0005 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 54.0885 | 17.9592 | 8.2009 | 21.8672 | 36.0659 | 32.4351 | 15.7141 | 8.4848 | 19.9305 | 39.1083 |
Translational Efficiency | 0.583 | 0.6171 | 0.6439 | 0.6279 | 0.6454 | 1.1998 | 0.7969 | 0.8245 | 0.5792 | 0.7226 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2520 | 1041 | 108 | 692 | 1719 | 1437 | 1819 | 1176 | 4239 | 2478 | 1927 | 1868 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 732.41 | 776.24 | 942.41 | 913.80 | 748.62 | 736.96 | 824.08 | 890.37 | 738.98 | 753.46 | 830.71 | 899.05 |
Standard Deviation | 100.48 | 107.21 | 105.64 | 113.38 | 97.45 | 100.65 | 107.94 | 126.60 | 99.58 | 105.26 | 111.20 | 122.39 |
Intensity Change Log 2 | — | 0.083851 | 0.363703 | 0.319227 | — | -0.022647 | 0.138551 | 0.250171 | — | 0.031002 | 0.254285 | 0.284734 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000272 | 0.000455 | 0.000798 | 0.002412 | 0.000365 | 0.001149 | 0.001134 | 0.001745 | 0.000309 | 0.000858 | 0.001115 | 0.001992 |
Bud Neck | 0.014365 | 0.008880 | 0.002685 | 0.009769 | 0.010513 | 0.023802 | 0.020616 | 0.012341 | 0.012803 | 0.017533 | 0.019611 | 0.011388 |
Bud Site | 0.006385 | 0.007513 | 0.012383 | 0.039758 | 0.010638 | 0.020035 | 0.033306 | 0.031215 | 0.008110 | 0.014775 | 0.032133 | 0.034380 |
Cell Periphery | 0.000554 | 0.000486 | 0.000600 | 0.001076 | 0.000706 | 0.000812 | 0.000632 | 0.001020 | 0.000615 | 0.000675 | 0.000630 | 0.001041 |
Cytoplasm | 0.425504* | 0.435691* | 0.378779* | 0.347705* | 0.423618* | 0.338681* | 0.474218* | 0.436949* | 0.424739* | 0.379435* | 0.468869* | 0.403889* |
Cytoplasmic Foci | 0.106238 | 0.205199* | 0.062469 | 0.059214 | 0.168376 | 0.246264* | 0.049083 | 0.077721 | 0.131436 | 0.229013* | 0.049833 | 0.070865 |
Eisosomes | 0.000607 | 0.000420 | 0.000162 | 0.000417 | 0.000774 | 0.000986 | 0.000231 | 0.000501 | 0.000675 | 0.000748 | 0.000227 | 0.000470 |
Endoplasmic Reticulum | 0.101989* | 0.044188 | 0.023487 | 0.036519 | 0.107495* | 0.051071 | 0.029985 | 0.035121 | 0.104222* | 0.048180 | 0.029621 | 0.035639 |
Endosome | 0.012954 | 0.020258 | 0.013562 | 0.008251 | 0.014664 | 0.033360 | 0.006267 | 0.006856 | 0.013648 | 0.027856 | 0.006676 | 0.007373 |
Golgi | 0.006619 | 0.005455 | 0.000303 | 0.001795 | 0.008824 | 0.015768 | 0.000878 | 0.002095 | 0.007513 | 0.011436 | 0.000845 | 0.001984 |
Lipid Particles | 0.049364 | 0.041733 | 0.009874 | 0.012755 | 0.061353 | 0.074701 | 0.009977 | 0.015998 | 0.054226 | 0.060851 | 0.009972 | 0.014797 |
Mitochondria | 0.005461 | 0.002888 | 0.000349 | 0.002058 | 0.006065 | 0.013846 | 0.002020 | 0.002916 | 0.005706 | 0.009242 | 0.001926 | 0.002598 |
Mitotic Spindle | 0.000297 | 0.000567 | 0.004940 | 0.010328 | 0.001557 | 0.003258 | 0.009697 | 0.006049 | 0.000808 | 0.002127 | 0.009430 | 0.007634 |
None | 0.008565 | 0.002210 | 0.001750 | 0.001162 | 0.004888 | 0.002468 | 0.002410 | 0.004719 | 0.007074 | 0.002359 | 0.002373 | 0.003401 |
Nuclear Periphery | 0.004984 | 0.002167 | 0.003461 | 0.002124 | 0.002890 | 0.001628 | 0.004627 | 0.002334 | 0.004135 | 0.001855 | 0.004562 | 0.002256 |
Nuclear Periphery Foci | 0.003937 | 0.006858 | 0.021832 | 0.021216 | 0.005403 | 0.002648 | 0.010234 | 0.015267 | 0.004532 | 0.004416 | 0.010884 | 0.017471 |
Nucleolus | 0.001918 | 0.001036 | 0.000643 | 0.000272 | 0.000920 | 0.001089 | 0.000427 | 0.000431 | 0.001513 | 0.001067 | 0.000439 | 0.000372 |
Nucleus | 0.170189* | 0.106040 | 0.279104* | 0.165484* | 0.102446 | 0.078009 | 0.215436* | 0.175394* | 0.142718* | 0.089785 | 0.219004* | 0.171722* |
Peroxisomes | 0.003021 | 0.004624 | 0.001779 | 0.001173 | 0.003499 | 0.011148 | 0.001816 | 0.002469 | 0.003215 | 0.008407 | 0.001814 | 0.001989 |
Vacuole | 0.075608 | 0.101807 | 0.180767 | 0.276016* | 0.063456 | 0.076059 | 0.126007 | 0.168344 | 0.070680 | 0.086876 | 0.129076 | 0.208231* |
Vacuole Periphery | 0.001169 | 0.001526 | 0.000273 | 0.000497 | 0.001549 | 0.003218 | 0.001002 | 0.000516 | 0.001323 | 0.002507 | 0.000961 | 0.000509 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.80 | -3.91 | -4.16 | -3.70 | -2.64 | -4.69 | -3.89 | -5.79 | -1.90 | -1.69 | -5.44 | -4.34 | -7.08 | -4.08 | -2.31 |
Bud Neck | 4.45 | 10.78 | 2.93 | -0.80 | -5.82 | -6.78 | -4.57 | -0.67 | 5.77 | 3.71 | -3.70 | -3.23 | 1.60 | 4.73 | 4.10 |
Bud Site | -0.81 | -0.92 | -6.24 | -5.90 | -3.56 | -3.50 | -6.52 | -5.24 | -2.14 | 0.66 | -4.26 | -8.13 | -8.60 | -5.46 | -0.18 |
Cell Periphery | 1.57 | -0.69 | -4.50 | -5.09 | -2.61 | -0.90 | 1.73 | -2.07 | -0.61 | -3.32 | -0.84 | 0.39 | -4.71 | -2.69 | -4.31 |
Cytoplasm | -1.07 | 3.03 | 10.68 | 9.99 | 1.37 | 10.25 | -4.14 | 1.67 | -6.92 | 5.05 | 7.56 | -3.90 | 7.41 | 0.89 | 9.23 |
Cytoplasmic Foci | -16.74 | 6.50 | 14.33 | 25.96 | 0.77 | -12.43 | 27.67 | 19.26 | 29.36 | -6.87 | -22.62 | 27.56 | 20.82 | 38.23 | -5.54 |
Eisosomes | 3.78 | 9.80 | 4.79 | 1.39 | -4.18 | -2.29 | 8.09 | 4.35 | 7.35 | -5.51 | -1.34 | 11.74 | 5.93 | 7.04 | -6.13 |
Endoplasmic Reticulum | 10.79 | 11.17 | 15.12 | 3.16 | -0.84 | 9.24 | 14.72 | 13.65 | 4.64 | -0.79 | 13.96 | 20.60 | 20.25 | 5.55 | -0.87 |
Endosome | -5.77 | -0.54 | 7.20 | 10.20 | 3.56 | -10.44 | 11.81 | 11.65 | 15.58 | -0.55 | -12.14 | 12.63 | 13.50 | 18.50 | -0.20 |
Golgi | 1.61 | 15.48 | 9.10 | 5.45 | -1.95 | -5.87 | 14.50 | 9.67 | 12.00 | -1.57 | -5.19 | 18.67 | 12.36 | 12.93 | -1.78 |
Lipid Particles | 2.81 | 18.27 | 20.06 | 12.56 | -0.45 | -3.58 | 22.64 | 19.52 | 18.92 | -4.18 | -2.67 | 30.26 | 27.02 | 22.21 | -3.70 |
Mitochondria | 4.56 | 19.56 | 5.77 | 1.74 | -1.63 | -5.00 | 5.27 | 3.29 | 7.12 | -0.56 | -3.94 | 8.72 | 5.44 | 7.09 | -0.40 |
Mitotic Spindle | -1.47 | -1.55 | -4.15 | -4.01 | -1.53 | -1.46 | -4.35 | -3.04 | -1.54 | 1.67 | -2.10 | -5.21 | -5.49 | -3.89 | 0.97 |
None | 6.56 | 6.83 | 7.88 | 3.45 | 1.09 | 2.98 | 3.43 | 0.76 | -1.14 | -1.41 | 6.88 | 6.52 | 4.51 | -0.60 | -0.85 |
Nuclear Periphery | 5.17 | 1.95 | 5.04 | -0.56 | 2.64 | 3.09 | -2.70 | 1.01 | -2.19 | 3.64 | 6.31 | -0.69 | 4.50 | -2.13 | 4.10 |
Nuclear Periphery Foci | -4.75 | -6.01 | -9.43 | -7.19 | 1.24 | 5.78 | -5.42 | -7.87 | -10.12 | -3.40 | 0.28 | -7.79 | -12.36 | -12.13 | -4.45 |
Nucleolus | 4.07 | 5.89 | 9.20 | 6.32 | 3.45 | -1.26 | 6.08 | 5.24 | 4.48 | -0.25 | 3.09 | 9.07 | 9.74 | 7.02 | 1.24 |
Nucleus | 13.79 | -4.73 | 1.62 | -6.86 | 5.06 | 6.54 | -20.54 | -11.99 | -16.10 | 5.74 | 17.41 | -14.54 | -5.30 | -16.60 | 8.00 |
Peroxisomes | -3.33 | 2.35 | 8.18 | 8.39 | 1.03 | -11.55 | 4.56 | 2.55 | 11.38 | -0.81 | -11.78 | 4.25 | 4.43 | 13.12 | -0.01 |
Vacuole | -6.55 | -6.81 | -24.83 | -21.50 | -6.30 | -3.65 | -18.20 | -21.72 | -19.47 | -8.13 | -6.27 | -18.64 | -31.47 | -27.85 | -15.06 |
Vacuole Periphery | -1.48 | 13.00 | 7.24 | 4.73 | -0.89 | -4.23 | 1.94 | 4.93 | 7.60 | 1.40 | -4.97 | 1.68 | 7.59 | 8.82 | 1.50 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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