Standard name
Human Ortholog
Description Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; long-lived protein that is preferentially retained in mother cells and forms cytoplasmic foci; oligomer activation requires heat-induced conformational change; also has mRNA binding activity
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.93 0.99 0.98 0.97 0.88 0.82 – 0.7 0.6 0.48 0.48 0.97 0.97 0.99 0.95 0.89 0.91 0.82 0.88 0.84 0.85 0.82 0.78
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.05 0 0 0 0.22 0.06 – 0.15 0.12 0.26 0.18 0 0 0 0 0.05 0.05 0.12 0 0.05 0 0 0.05
Nucleus 0 0 0 0 0 0 0 – 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.13 – 0.19 0.21 0.24 0.23 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 1 – 1 3 3 4 0 0 0 1 0 0 0 0 0 0 1 2
Bud 0 1 0 0 0 5 3 – 8 11 15 6 0 0 0 1 0 0 0 1 0 2 8 7
Bud Neck 1 0 0 3 3 0 2 – 0 1 0 0 0 0 0 0 0 0 1 0 0 1 2 1
Bud Site 0 0 0 0 0 0 2 – 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 0 0 1 0 1 – 3 3 4 8 0 1 1 0 0 2 0 0 0 0 0 1
Cytoplasm 144 164 85 177 220 133 230 – 137 147 102 112 121 176 248 91 49 58 140 158 76 104 203 154
Endoplasmic Reticulum 0 2 0 0 0 0 1 – 0 3 8 12 1 0 1 4 2 3 0 0 0 1 2 1
Endosome 0 0 1 0 2 1 2 – 0 2 1 1 0 2 1 3 2 2 1 5 0 1 3 5
Golgi 0 0 0 0 0 0 1 – 1 3 7 4 0 1 0 1 1 0 0 0 0 0 1 2
Mitochondria 12 9 0 1 1 33 16 – 30 29 55 41 2 1 0 4 3 3 19 6 4 4 10 9
Nucleus 0 2 0 2 1 4 5 – 8 8 10 13 1 0 0 1 0 0 0 0 0 0 0 2
Nuclear Periphery 0 0 0 0 0 1 4 – 2 4 6 7 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 1 – 2 5 4 6 0 0 0 0 1 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 3 – 3 6 4 1 0 0 0 0 0 0 1 0 2 0 3 2
Vac/Vac Membrane 1 2 0 2 7 4 37 – 37 51 51 54 1 2 1 0 0 1 2 4 0 0 2 2
Unique Cell Count 149 176 86 180 226 152 282 197 246 214 231 125 182 250 96 55 64 172 180 91 123 248 199
Labelled Cell Count 158 183 86 186 235 182 309 233 277 270 269 126 183 252 106 59 69 172 180 91 123 248 199


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.6 5.3 4.6 5.7 4.6 8.5 – 16.0 15.5 16.3 19.7 4.7 5.0 6.2 5.4 5.7 5.4 13.0 6.6 12.4
Std Deviation (1e-4) 0.3 1.7 2.8 1.4 2.3 2.5 6.8 – 19.4 16.4 22.8 23.6 1.7 1.8 3.2 1.3 1.7 1.2 36.7 18.4 41.0
Intensity Change (Log2) – – – -0.18 0.12 -0.2 0.69 – 1.61 1.56 1.63 1.9 -0.16 -0.07 0.24 0.04 0.11 0.03 1.31 0.33 1.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2235 10.0043 3.5928 5.2461 7.8135 2.4988 6.7522 13.4383 7.223 8.0801 5.7058 9.031 6.4309 11.404 7.9538 9.5494 8.1453 10.261
Actin 0.0307 0.0314 0.0387 0.0164 0.0243 0.0209 0.0217 0.0145 0.0328 0.0677 0.0397 0.0167 0.0272 0.0365 0.019 0.0158 0.0041 0.0307
Bud 0.0123 0.0149 0.0115 0.0065 0.0015 0.0036 0.0069 0.0226 0.009 0.0125 0.0014 0.0019 0.0056 0.0127 0.0057 0.0124 0.0078 0.0054
Bud Neck 0.0039 0.0015 0.0012 0.001 0.0048 0.0035 0.005 0.0016 0.0031 0.0041 0.0012 0.0259 0.0028 0.0028 0.0059 0.0235 0.0029 0.0044
Bud Periphery 0.0062 0.0086 0.0064 0.0061 0.0011 0.0042 0.0046 0.0097 0.0114 0.007 0.0013 0.0016 0.0046 0.012 0.004 0.0087 0.0069 0.0083
Bud Site 0.0217 0.0545 0.0173 0.0024 0.0025 0.0015 0.0143 0.036 0.0149 0.0071 0.0039 0.0021 0.0077 0.0447 0.0059 0.0052 0.0036 0.0044
Cell Periphery 0.0006 0.0012 0.0008 0.0003 0.0002 0.0005 0.0007 0.0016 0.0004 0.0003 0.0003 0.0002 0.0005 0.0012 0.0012 0.0006 0.0002 0.0021
Cytoplasm 0.1844 0.2625 0.2179 0.2211 0.0805 0.2344 0.2499 0.3792 0.2614 0.3229 0.1636 0.2782 0.2874 0.2683 0.2623 0.2896 0.3412 0.2904
Cytoplasmic Foci 0.104 0.2061 0.1182 0.2475 0.2116 0.1207 0.1366 0.1601 0.1802 0.1402 0.1354 0.1814 0.097 0.2026 0.1687 0.1055 0.1738 0.1533
Eisosomes 0.0003 0.0002 0.0003 0.0013 0.0002 0.0001 0.0004 0.0002 0.0001 0.0002 0.0001 0.0001 0.0003 0.0002 0.0001 0.0001 0 0.0002
Endoplasmic Reticulum 0.0108 0.0108 0.0231 0.0047 0.007 0.0123 0.0241 0.012 0.0193 0.0123 0.0254 0.0176 0.0189 0.0138 0.0201 0.0172 0.0097 0.0121
Endosome 0.1551 0.1584 0.127 0.1441 0.1249 0.1641 0.1655 0.1109 0.1727 0.1567 0.2975 0.1796 0.1146 0.1031 0.1425 0.0841 0.2031 0.1489
Golgi 0.0357 0.0293 0.0351 0.0153 0.0846 0.0421 0.0332 0.0271 0.0411 0.0418 0.1157 0.0337 0.025 0.0217 0.0351 0.0073 0.0121 0.0367
Lipid Particles 0.0239 0.0083 0.0096 0.0345 0.0069 0.0159 0.0141 0.0082 0.0067 0.003 0.0264 0.0114 0.0115 0.0069 0.0113 0.004 0.0108 0.0058
Mitochondria 0.0215 0.0106 0.0188 0.0059 0.0832 0.0095 0.012 0.0091 0.0252 0.0243 0.0383 0.0125 0.0088 0.0072 0.0066 0.0144 0.0049 0.0147
None 0.176 0.0806 0.1671 0.149 0.3145 0.2024 0.0552 0.0309 0.0233 0.0466 0.005 0.0404 0.161 0.108 0.0998 0.1502 0.0422 0.0797
Nuclear Periphery 0.0614 0.0102 0.0509 0.0259 0.0033 0.0367 0.0754 0.0165 0.0429 0.022 0.0273 0.0424 0.0557 0.0303 0.0416 0.0563 0.0167 0.0348
Nucleolus 0.0047 0.0181 0.0025 0.0073 0.0042 0.0007 0.0085 0.0094 0.0039 0.001 0.0011 0.0048 0.0044 0.0195 0.0082 0.0018 0.0116 0.0139
Nucleus 0.0742 0.05 0.1118 0.0646 0.0023 0.0686 0.0901 0.0838 0.0784 0.1 0.0581 0.0729 0.1185 0.0473 0.0996 0.1209 0.0789 0.0817
Peroxisomes 0.0118 0.0183 0.0156 0.0202 0.0174 0.0152 0.0142 0.0198 0.0261 0.01 0.0315 0.0332 0.0071 0.0348 0.0237 0.0332 0.011 0.0237
Punctate Nuclear 0.0398 0.0094 0.0148 0.0151 0.0194 0.0256 0.0287 0.0256 0.0275 0.0085 0.0069 0.0244 0.0267 0.0173 0.025 0.0336 0.0413 0.0309
Vacuole 0.0156 0.0125 0.0077 0.0079 0.0026 0.0136 0.0307 0.0169 0.0149 0.0088 0.0126 0.0134 0.0106 0.0072 0.01 0.0096 0.0129 0.0116
Vacuole Periphery 0.0055 0.0027 0.0035 0.0028 0.0033 0.0041 0.0081 0.0046 0.0047 0.0033 0.0073 0.0057 0.0043 0.0018 0.0036 0.0061 0.0045 0.0064

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.1196 4.5878 10.213 12.6027 8.872 8.5488 8.8909 18.7849 17.7045 14.9157
Translational Efficiency 1.692 3.4192 1.5295 1.6778 2.1884 2.2362 2.4186 1.162 2.3103 1.9065

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
477 1068 394 1845 1790 1977 153 1222 2267 3045 547 3067

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 863.66 781.25 2505.71 851.86 666.08 767.22 1123.23 983.95 707.65 772.14 2119.02 904.49
Standard Deviation 615.14 324.53 1778.16 223.32 79.42 127.23 480.41 629.52 301.80 217.93 1651.38 438.27
Intensity Change Log 2 — -0.144679 1.536684 -0.019847 — 0.203945 0.753886 0.562890 — 0.017557 1.246262 0.263130

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.004150 0.000816 0.000842 0.000581 0.000081 0.000403 0.005747 0.001001 0.000937 0.000548 0.002214 0.000748
Bud Neck 0.019896 0.022242 0.017799 0.004512 0.015109 0.043262 0.004130 0.008148 0.016116 0.035890 0.013976 0.005961
Bud Site 0.054822 0.039538 0.022737 0.020590 0.003865 0.025236 0.029517 0.029321 0.014587 0.030253 0.024633 0.024069
Cell Periphery 0.001369 0.000264 0.001529 0.000284 0.000142 0.000258 0.000307 0.000451 0.000400 0.000260 0.001187 0.000351
Cytoplasm 0.415616* 0.487751* 0.407329* 0.597596* 0.525722* 0.473928* 0.366633* 0.583656* 0.502555* 0.478777* 0.395946* 0.592042*
Cytoplasmic Foci 0.286986* 0.212067* 0.109402 0.025833 0.191273* 0.192753* 0.043397 0.026442 0.211412* 0.199527* 0.090940 0.026075
Eisosomes 0.000250 0.000081 0.000019 0.000051 0.000174 0.000150 0.000112 0.000043 0.000190 0.000126 0.000045 0.000047
Endoplasmic Reticulum 0.005426 0.001329 0.001518 0.001168 0.000443 0.000932 0.006450 0.001033 0.001491 0.001071 0.002898 0.001114
Endosome 0.013804 0.025050 0.002768 0.005894 0.006953 0.017751 0.005959 0.006445 0.008394 0.020311 0.003661 0.006113
Golgi 0.005960 0.006052 0.003250 0.003572 0.001120 0.004843 0.000440 0.005179 0.002138 0.005267 0.002464 0.004212
Lipid Particles 0.011560 0.005443 0.007982 0.001114 0.007703 0.005644 0.004622 0.001210 0.008514 0.005574 0.007042 0.001152
Mitochondria 0.010008 0.004578 0.001509 0.001503 0.002227 0.008599 0.000450 0.001547 0.003864 0.007189 0.001213 0.001520
Mitotic Spindle 0.013005 0.008428 0.043603 0.023494 0.000194 0.012466 0.004618 0.065429 0.002890 0.011049 0.032698 0.040202
None 0.004592 0.008925 0.009372 0.013217 0.003295 0.005978 0.005809 0.008555 0.003568 0.007012 0.008376 0.011360
Nuclear Periphery 0.000444 0.000561 0.002178 0.000792 0.000323 0.000364 0.001534 0.000871 0.000348 0.000433 0.001998 0.000824
Nuclear Periphery Foci 0.003545 0.002433 0.006486 0.001187 0.000199 0.000458 0.007484 0.001525 0.000903 0.001151 0.006765 0.001321
Nucleolus 0.002935 0.002645 0.000706 0.000621 0.000698 0.002330 0.000531 0.000949 0.001169 0.002441 0.000657 0.000752
Nucleus 0.100845 0.072197 0.312741* 0.187055* 0.201382* 0.130148* 0.295643* 0.161811* 0.180228* 0.109822 0.307958* 0.176997*
Peroxisomes 0.009501 0.013988 0.001711 0.001529 0.004618 0.010126 0.007331 0.002008 0.005646 0.011481 0.003283 0.001720
Vacuole 0.032045 0.082907 0.045148 0.107069 0.034066 0.062621 0.208942* 0.090942 0.033641 0.069736 0.090962 0.100644
Vacuole Periphery 0.003244 0.002703 0.001370 0.002339 0.000414 0.001749 0.000343 0.003435 0.001009 0.002083 0.001083 0.002776

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.75 1.65 1.97 2.49 4.11 -4.48 -1.35 -3.05 -1.09 1.10 0.97 -0.84 1.12 0.60 1.78
Bud Neck -0.51 -0.23 6.29 10.23 6.98 -10.59 6.88 4.87 14.27 -2.45 -9.87 -0.18 8.58 17.70 6.04
Bud Site 2.35 4.79 6.10 6.40 2.64 -10.70 -2.29 -7.73 2.01 -1.05 -6.64 -2.11 -1.07 6.18 1.39
Cell Periphery 2.88 -1.08 3.10 2.59 8.55 -3.72 -3.29 -1.98 0.14 0.79 1.66 -6.15 2.25 1.59 8.31
Cytoplasm -4.26 2.81 -6.89 -2.73 -10.82 5.95 11.33 4.52 -0.57 -8.64 3.28 13.28 0.16 -3.20 -13.32
Cytoplasmic Foci 5.44 13.02 22.16 27.04 6.61 -0.25 24.63 32.92 32.69 -1.15 2.03 16.95 41.03 43.75 6.02
Eisosomes 8.38 11.61 10.25 6.92 -9.14 1.57 5.14 10.49 10.46 6.07 5.12 13.26 13.42 11.64 -0.89
Endoplasmic Reticulum 3.31 3.04 3.45 0.63 1.28 -1.19 -7.87 -11.88 -1.22 6.54 1.15 -3.48 0.71 -0.88 5.11
Endosome -4.46 5.84 5.03 11.81 -2.10 -9.24 1.75 3.35 11.94 1.53 -11.00 6.80 5.75 16.98 -1.09
Golgi 0.09 2.90 3.75 4.02 1.61 -5.60 5.39 -0.82 2.73 -1.72 -5.43 -0.15 0.64 4.75 0.69
Lipid Particles 3.78 1.90 7.62 4.62 4.66 2.53 3.91 9.41 8.65 3.77 3.97 1.48 11.95 9.45 5.27
Mitochondria 2.56 4.03 4.31 5.39 2.23 -6.66 4.48 3.16 8.77 -3.19 -4.04 4.75 5.20 9.98 1.72
Mitotic Spindle 1.40 -3.47 -3.99 -6.68 0.80 -6.74 -3.42 -12.06 -9.45 -10.43 -5.39 -5.87 -14.35 -11.04 -3.41
None -2.93 -3.23 -3.55 -0.35 -1.11 -2.91 -1.96 -5.94 -1.48 -1.41 -4.34 -5.21 -6.35 -1.62 -1.48
Nuclear Periphery -0.69 -9.02 -10.53 -5.77 5.94 -1.02 -10.33 -19.28 -16.43 2.22 -1.54 -11.64 -23.02 -15.38 6.11
Nuclear Periphery Foci 0.70 -2.47 1.11 0.52 4.07 -2.43 -4.17 -6.89 -5.73 2.89 -1.68 -6.78 -6.09 -4.42 4.84
Nucleolus 0.30 2.44 2.25 2.99 -0.85 -5.13 2.76 -2.84 3.55 -4.44 -4.00 2.32 0.58 4.87 -2.69
Nucleus 3.84 -16.66 -10.41 -19.31 11.50 12.27 -5.84 1.53 -8.91 6.27 14.76 -13.59 -1.42 -17.23 13.07
Peroxisomes -2.62 7.89 7.91 9.26 0.01 -5.26 1.28 4.59 9.81 0.87 -6.40 5.94 7.75 13.77 0.62
Vacuole -10.47 -6.86 -25.19 -15.15 -11.04 -10.19 -11.59 -21.66 -16.63 4.75 -15.01 -12.19 -34.93 -24.45 -4.38
Vacuole Periphery 0.90 2.91 2.75 2.76 0.26 -8.23 2.21 -1.40 0.78 -1.68 -4.97 0.11 -0.51 2.27 -0.56
Standard name
Human Ortholog
Description Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; long-lived protein that is preferentially retained in mother cells and forms cytoplasmic foci; oligomer activation requires heat-induced conformational change; also has mRNA binding activity
Localization
Cell Percentages cytoplasm (23%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Hsp26

Hsp26


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hsp26-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available