Standard name
Human Ortholog
Description DNA-dependent ATPase; DNA recombination/repair translocase, supercoils DNA and promotes DNA strand opening; stimulates strand exchange by modifying dsDNA topology; involved in recombinational repair of DNA double-strand breaks (DSBs) during mitosis and meiosis; phosphorylated in Mec1p-, Rad53p-dependent way in response to one DSB; contributes to remodelling of nucleosomes; proposed to be involved in crossover interference; interacts with Dmc1p; stimulates Dmc1p and Rad51p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0.05 0.07 0.08 0.07 0.15 0.17 0.18 0.19 0.23 0.2 0.08 0.13 0.2 0.05 0.09 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.27 0.37 0.48 0.27 0.63 0.66 0.6 0.75 0.74 0.69 0.78 0.09 0 0.18 0 0 0.05 0 0 0 0 0 0
Nucleus 0.95 0.82 0.82 0.79 0.78 0.68 0.55 0.5 0.41 0.38 0.4 0.34 0.87 0.78 0.59 0.84 0.8 0.77 0.85 0.82 0.77 0.65 0.61 0.34
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.06 0.05 0.08 0.06 0 0 0 0 0.08
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.06 0 0 0 0 0 0 0 0 0 0.05 0 0 0.07 0.07 0.06 0.07 0 0.07 0.13 0.21 0.24 0.36
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 2 8 7 8 9 9 12 0 0 0 1 1 0 1 3 2 2 2 5
Bud Neck 0 0 0 0 0 0 0 0 0 2 2 1 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 3 2 14 11 32 15 21 20 28 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 7 17 20 31 32 84 138 69 79 96 106 10 29 42 17 26 25 0 2 3 0 1 2
Endoplasmic Reticulum 0 0 0 0 0 1 1 0 0 0 0 0 0 0 3 6 7 8 0 0 2 0 1 1
Endosome 0 0 1 0 0 1 0 3 0 0 0 0 0 4 4 2 3 1 1 1 1 1 3 11
Golgi 2 0 0 0 0 0 0 0 0 0 0 0 0 2 2 0 1 1 1 0 0 0 0 1
Mitochondria 4 38 117 136 99 294 361 496 279 310 293 406 11 9 38 11 3 13 1 1 7 0 4 8
Nucleus 191 115 258 223 290 316 298 416 153 158 167 178 109 170 124 316 223 216 178 114 247 58 91 67
Nuclear Periphery 1 2 2 3 5 6 8 4 1 5 5 3 2 7 1 4 6 4 1 0 2 0 1 4
Nucleolus 2 2 4 10 11 21 19 34 10 16 15 17 5 6 8 24 13 23 13 1 7 1 4 14
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 2 2 3 3 16 5 6 2 10 0 2 1 2 3 1 2 2 3 1 1 2
Vac/Vac Membrane 6 8 6 2 13 12 19 31 6 4 4 25 3 8 14 27 17 21 7 9 41 18 35 70
Unique Cell Count 200 141 315 282 370 466 545 830 374 418 422 520 125 217 211 377 278 282 211 141 323 90 150 197
Labelled Cell Count 208 172 407 399 454 702 812 1177 546 610 613 786 140 237 237 411 303 313 211 141 323 90 150 197


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 4.4 4.3 4.2 5.3 3.7 3.6 3.5 2.9 2.9 3.0 2.6 5.7 5.7 5.0 8.6 9.6 9.7 5.1 5.5 6.2
Std Deviation (1e-4) 1.1 1.0 1.1 1.5 3.6 1.2 1.6 1.2 1.2 1.3 1.3 1.1 1.4 1.6 1.7 2.1 2.4 2.2 0.8 1.3 1.8
Intensity Change (Log2) – – – -0.03 0.3 -0.19 -0.26 -0.31 -0.56 -0.56 -0.52 -0.71 0.41 0.43 0.22 1.01 1.17 1.18 0.25 0.38 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.2 2.6 3.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8282 3.0531 3.269 2.6616 2.1146 2.3995 2.4085 3.7218 3.8598 2.6745 3.0012 2.9131 2.5487 4.3065 4.1218 3.5422 3.2973 3.5906
Actin 0.0176 0 0.0078 0 0.0096 0.0001 0.0312 0.0002 0.0109 0 0.0026 0.0015 0.0068 0.0001 0.0001 0.0002 0 0.0005
Bud 0.0002 0 0.0001 0 0.0001 0 0.0004 0.0002 0.0004 0 0.0004 0.0002 0.0001 0 0 0 0 0.0001
Bud Neck 0.0012 0.0002 0.0006 0.0003 0.0003 0.0018 0.0041 0.0008 0.0009 0.0002 0.001 0.0017 0.0007 0.0002 0.0005 0.0002 0.0007 0.0029
Bud Periphery 0.0004 0 0.0003 0 0.0001 0.0001 0.0012 0.0006 0.002 0 0.0004 0.0006 0.0002 0 0.0001 0.0001 0 0.0002
Bud Site 0.0007 0.0001 0.0003 0 0.0002 0.0001 0.0019 0.0022 0.0017 0 0.013 0.0004 0.0004 0.0002 0.0002 0.0001 0.0001 0.0003
Cell Periphery 0.0001 0 0.0001 0 0 0 0.0004 0.0002 0.0002 0 0.0002 0.0001 0 0 0 0 0 0.0001
Cytoplasm 0.003 0.0009 0.0014 0.0001 0.0001 0.0001 0.0036 0.0007 0.0013 0.0001 0.0109 0.0018 0.0081 0.0016 0.0013 0.0004 0.0013 0.0016
Cytoplasmic Foci 0.0055 0 0.0009 0 0.0021 0 0.0103 0.001 0.0022 0 0.0234 0.0021 0.0019 0 0.0016 0 0 0.0002
Eisosomes 0.0002 0 0.0001 0 0.0001 0 0.0003 0 0.0002 0 0.0001 0.0001 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0018 0.0001 0.001 0 0.0001 0.0001 0.0012 0.0003 0.0022 0 0.0033 0.001 0.001 0.0002 0.0002 0.0002 0.0001 0.0002
Endosome 0.0138 0.0001 0.0044 0.0001 0.0011 0.0006 0.0092 0.0142 0.0108 0 0.0089 0.0111 0.0056 0.0002 0.0005 0.0004 0.0002 0.0017
Golgi 0.0041 0 0.0015 0 0.0045 0 0.0029 0.0011 0.0033 0 0.0021 0.0018 0.0007 0 0.0003 0 0 0.0002
Lipid Particles 0.0059 0 0.0042 0 0.011 0.0001 0.0037 0.0007 0.0089 0 0.0182 0.0074 0.0008 0 0.014 0.0001 0 0.0003
Mitochondria 0.0056 0.0001 0.0049 0.0001 0.0052 0.0022 0.0175 0.0033 0.0137 0.0002 0.0015 0.0086 0.001 0.0004 0.0005 0.0006 0.0002 0.0009
None 0.0133 0.0004 0.001 0 0.0001 0.0001 0.0045 0.0001 0.0017 0.0001 0.0042 0.0002 0.0135 0.0002 0.0014 0.0002 0.0014 0.0022
Nuclear Periphery 0.0321 0.0094 0.0317 0.011 0.0056 0.0061 0.0268 0.0106 0.0285 0.0046 0.0167 0.0066 0.0122 0.0238 0.0255 0.0441 0.0034 0.0046
Nucleolus 0.0429 0.0313 0.0506 0.0701 0.0403 0.0549 0.0424 0.04 0.0513 0.0446 0.0418 0.0547 0.0432 0.0369 0.0418 0.0401 0.0393 0.0915
Nucleus 0.8087 0.9442 0.8742 0.9049 0.8838 0.9265 0.8008 0.909 0.844 0.9466 0.8348 0.8801 0.8489 0.9212 0.9026 0.9034 0.9454 0.8793
Peroxisomes 0.0064 0 0.0006 0 0.0217 0 0.0031 0.0002 0.0036 0 0.0081 0.0015 0.0007 0 0.0002 0 0 0.0002
Punctate Nuclear 0.032 0.0126 0.0125 0.0128 0.0135 0.0058 0.0275 0.0088 0.0079 0.0034 0.0064 0.0124 0.0529 0.0139 0.0084 0.0089 0.0072 0.0111
Vacuole 0.0024 0.0003 0.0008 0.0002 0.0002 0.0005 0.004 0.0047 0.0021 0.0001 0.0018 0.0036 0.0009 0.0003 0.0004 0.0003 0.0002 0.0017
Vacuole Periphery 0.0021 0.0002 0.0013 0.0002 0.0004 0.0006 0.0029 0.0013 0.0021 0.0001 0.0007 0.0024 0.0005 0.0006 0.0004 0.0006 0.0001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 58.6795 22.628 16.0277 27.5135 44.7532 37.1814 22.8792 17.8986 20.3956 43.9408
Translational Efficiency 0.6725 0.6792 0.611 0.5458 0.6349 1.0165 0.6322 0.5999 0.5597 0.6429

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
88 70 535 1665 1192 1442 975 853 1280 1512 1510 2518

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 719.90 829.15 1016.68 859.96 709.03 854.69 1029.41 1085.89 709.78 853.51 1024.90 936.50
Standard Deviation 120.50 142.74 156.32 105.85 109.85 118.92 129.25 172.68 110.65 120.25 139.57 170.13
Intensity Change Log 2 — 0.203837 0.497997 0.256473 — 0.269555 0.537899 0.614959 — 0.236820 0.517934 0.445465

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000232 0.001496 0.001179 0.000606 0.000234 0.000315 0.000980 0.000257 0.000234 0.000369 0.001051 0.000488
Bud Neck 0.001807 0.004780 0.022604 0.010234 0.003138 0.007744 0.014651 0.031589 0.003047 0.007606 0.017469 0.017468
Bud Site 0.001273 0.001120 0.003267 0.000857 0.001403 0.001230 0.001273 0.005465 0.001394 0.001225 0.001980 0.002418
Cell Periphery 0.000131 0.000175 0.000077 0.000051 0.000196 0.000066 0.000066 0.000333 0.000191 0.000071 0.000070 0.000147
Cytoplasm 0.036160 0.005931 0.003348 0.002375 0.022629 0.005909 0.002405 0.062850 0.023560 0.005910 0.002739 0.022862
Cytoplasmic Foci 0.006505 0.002064 0.000815 0.000139 0.004225 0.001235 0.000047 0.000184 0.004381 0.001273 0.000319 0.000154
Eisosomes 0.000033 0.000075 0.000033 0.000025 0.000024 0.000019 0.000030 0.000022 0.000025 0.000022 0.000031 0.000024
Endoplasmic Reticulum 0.002285 0.011857 0.004317 0.003715 0.002764 0.005456 0.005810 0.001419 0.002731 0.005753 0.005281 0.002937
Endosome 0.000377 0.001340 0.000739 0.000447 0.000614 0.001256 0.000389 0.000407 0.000597 0.001260 0.000513 0.000434
Golgi 0.000066 0.000455 0.000943 0.000137 0.000305 0.000795 0.000118 0.000097 0.000289 0.000779 0.000410 0.000123
Lipid Particles 0.001607 0.001758 0.000150 0.000240 0.001124 0.000656 0.000195 0.000051 0.001157 0.000707 0.000179 0.000176
Mitochondria 0.000547 0.013823 0.001622 0.002191 0.002632 0.005356 0.001586 0.002153 0.002489 0.005748 0.001599 0.002178
Mitotic Spindle 0.000088 0.001227 0.003733 0.005141 0.003035 0.007921 0.004117 0.012996 0.002833 0.007611 0.003981 0.007802
None 0.007772 0.006231 0.002473 0.002311 0.020757 0.003735 0.004231 0.012293 0.019864 0.003851 0.003608 0.005693
Nuclear Periphery 0.000813 0.001683 0.000805 0.001291 0.001478 0.002692 0.001109 0.001486 0.001433 0.002645 0.001002 0.001357
Nuclear Periphery Foci 0.000483 0.003952 0.000937 0.001796 0.001350 0.001057 0.000787 0.000092 0.001290 0.001191 0.000840 0.001219
Nucleolus 0.029132 0.083599 0.064807 0.102364 0.042576 0.050105 0.031912 0.008724 0.041652 0.051655 0.043566 0.070643
Nucleus 0.908735* 0.853065* 0.883765* 0.864033* 0.889000* 0.898480* 0.927606* 0.857241* 0.890356* 0.896378* 0.912073* 0.861732*
Peroxisomes 0.000632 0.002456 0.000927 0.000566 0.001336 0.000567 0.000400 0.000045 0.001287 0.000654 0.000587 0.000389
Vacuole 0.001215 0.001830 0.002355 0.001077 0.000988 0.004179 0.002106 0.002145 0.001003 0.004071 0.002194 0.001439
Vacuole Periphery 0.000108 0.001084 0.001102 0.000404 0.000192 0.001226 0.000181 0.000151 0.000186 0.001220 0.000508 0.000318

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.20 -6.11 -2.02 2.48 7.02 -2.91 -18.33 -1.36 2.44 18.89* -3.81 -21.71 -6.94 -4.01 14.39
Bud Neck -1.04 -10.87 -6.45 -4.36 8.20 -6.25 -15.25 -14.81 -11.79 -4.71 -6.35 -18.46 -16.64 -9.47 4.96
Bud Site 0.90 -1.81 0.94 0.10 4.31 -0.10 -2.79 -4.77 -6.01 -3.52 0.02 -3.60 -1.59 -2.63 3.15
Cell Periphery -0.50 1.01 2.37 2.65 4.36 5.75 5.08 -0.84 -5.72 -5.17 5.68 4.87 3.62 -3.37 -1.84
Cytoplasm 3.40 3.58 3.93 1.62 2.07 7.65 9.85 -7.48 -13.43 -14.81 8.29 9.93 1.97 -8.96 -11.80
Cytoplasmic Foci 1.25 1.69 1.82 1.44 1.09 3.14 4.54 4.44 3.12 -3.07 3.37 4.62 4.77 3.35 0.68
Eisosomes -1.02 0.86 1.66 3.86 5.18 2.28 -7.39 2.31 -0.05 10.34 1.75 -6.50 0.94 -1.26 9.95
Endoplasmic Reticulum -3.18 -2.13 -1.27 2.92 2.12 -6.35 -12.71 3.96 8.62 16.20 -7.00 -12.40 -2.02 6.10 11.39
Endosome -0.99 -0.02 0.92 1.36 0.88 -2.55 0.68 2.69 3.74 3.70 -2.66 0.34 2.20 3.68 1.77
Golgi -0.93 -1.06 0.48 1.39 1.23 -1.26 0.48 1.53 1.99 7.52 -1.30 -0.48 1.31 1.93 1.72
Lipid Particles 0.05 1.73 1.66 3.71 -0.71 2.13 5.74 7.45 4.04 7.35 2.13 6.06 6.13 3.23 0.90
Mitochondria -1.20 -9.30 -3.80 1.04 -0.56 -1.89 0.53 0.76 3.00 0.87 -2.24 0.48 0.42 2.98 -0.10
Mitotic Spindle -1.19 -3.67 -5.44 -4.57 0.86 -2.47 -4.37 -4.34 -2.27 -0.23 -2.54 -4.79 -4.36 -1.05 1.30
None 0.58 2.53 3.00 1.43 2.59 6.77 6.61 3.81 -7.50 -6.28 6.81 7.04 6.41 -1.99 -2.74
Nuclear Periphery -1.51 -2.49 -2.91 0.55 -0.78 -2.06 -1.23 -2.55 1.32 -1.41 -2.16 -0.73 -1.00 1.89 -0.39
Nuclear Periphery Foci -1.06 0.75 -0.95 0.42 -3.08 0.22 -0.74 4.23 3.73 3.90 0.02 -0.14 -1.70 -1.68 -1.52
Nucleolus -2.05 -3.70 -6.25 -2.11 -3.25 -1.96 -3.13 5.25 7.06 7.66 -2.35 -5.71 -9.15 -6.51 -2.85
Nucleus 1.26 3.28 2.80 0.68 -1.27 -0.66 2.01 4.16 4.86 2.10 -0.41 4.41 6.44 7.03 1.65
Peroxisomes -0.70 0.62 1.07 2.93 2.67 1.79 1.35 3.09 5.38 10.81 1.74 1.26 2.32 1.91 4.90
Vacuole -0.65 -3.86 0.84 1.40 4.01 -3.36 -4.00 -3.55 0.77 0.73 -3.37 -5.38 -2.83 2.41 4.52
Vacuole Periphery -1.20 -1.74 -1.77 0.84 0.98 -3.50 -2.03 3.17 3.75 6.63 -3.63 -1.79 -1.07 3.08 0.84
Standard name
Human Ortholog
Description DNA-dependent ATPase; DNA recombination/repair translocase, supercoils DNA and promotes DNA strand opening; stimulates strand exchange by modifying dsDNA topology; involved in recombinational repair of DNA double-strand breaks (DSBs) during mitosis and meiosis; phosphorylated in Mec1p-, Rad53p-dependent way in response to one DSB; contributes to remodelling of nucleosomes; proposed to be involved in crossover interference; interacts with Dmc1p; stimulates Dmc1p and Rad51p
Localization
Cell Percentages nucleus (94%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Rdh54

Rdh54


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rdh54-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available