Standard name
Human Ortholog
Description High-mobility group (HMG) protein; binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to the chromosomes; functionally redundant with Nhp6Ap; required for transcriptional initiation fidelity of some tRNA genes; homologous to mammalian HMGB1 and HMGB2; NHP6B has a paralog, NHP6A, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 – 0 0 0 0 0 0 0.08 0.1 0.1 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.07 – 0.06 0.07 0.06 0.08 0.08 0 0 0 0 0.12 0 0 0 0 0 0 0
Nucleus 0.95 0.94 0.91 0.85 0.84 0.75 – 0.66 0.68 0.67 0.67 0.68 0.93 0.81 0.7 0.83 0.82 0.94 0.95 0.91 0.92 0.79 0.59 0.43
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0.08 0.12 0.25 0.22 0.36 – 0.45 0.48 0.53 0.57 0.53 0.09 0.21 0.28 0.07 0.29 0.31 0 0 0.05 0.1 0.22 0.25
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.27
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 1 0 1 0 0 1 0 1 0 0 0 1 0 0 0 2 2
Bud Neck 0 0 0 0 0 0 – 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 – 4 13 4 4 20 0 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 8 5 1 3 11 11 – 18 17 10 7 8 11 33 46 3 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 1 1 0 – 0 0 0 0 1 0 1 3 0 0 0 0 1 0 0 5 4
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Mitochondria 3 2 1 10 2 30 – 46 42 44 36 52 0 8 3 0 2 0 0 0 0 0 0 4
Nucleus 307 154 94 251 392 308 – 543 389 464 284 430 302 336 317 25 14 15 311 156 96 48 186 177
Nuclear Periphery 0 1 0 1 0 2 – 3 3 4 5 2 0 0 0 0 0 0 0 0 0 0 0 2
Nucleolus 23 13 12 74 101 148 – 373 272 367 243 338 28 85 126 2 5 5 9 6 5 6 71 100
Peroxisomes 1 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 1 0 1 0 0 1 0 8 0 0 0 0 0 0 0 2 2
Vac/Vac Membrane 4 1 1 4 3 2 – 6 1 3 1 0 2 3 7 0 0 0 4 5 2 2 43 109
Unique Cell Count 324 163 103 295 466 408 822 569 691 424 632 326 413 455 30 17 16 328 173 105 62 316 408
Labelled Cell Count 346 176 109 344 510 501 996 737 899 581 852 346 466 513 30 21 20 328 173 105 62 316 408


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 27.7 33.4 17.6 17.9 21.4 19.3 – 17.9 17.6 17.0 15.9 15.6 30.7 33.0 33.1 47.7 46.6 45.6 27.2 28.5 32.0
Std Deviation (1e-4) 8.2 12.2 4.6 5.3 6.7 6.3 – 6.2 6.8 6.0 6.4 5.7 9.7 11.9 12.8 22.0 19.1 12.6 14.9 8.8 9.4
Intensity Change (Log2) – – – 0.03 0.29 0.13 – 0.02 -0.0 -0.05 -0.14 -0.18 0.8 0.91 0.91 1.44 1.4 1.37 0.63 0.69 0.86


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 – 0 0 0 0 0 0 2.6 3.0 0 0 0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.4 – 2.0 2.4 2.2 2.7 2.6 0 0 0 0 0 0
Nucleus -1.6 -1.9 -3.5 – -5.2 -4.8 -5.0 -4.9 -4.8 0.5 -2.4 -4.5 -1.2 -1.1 0.3
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.9 2.3 4.8 – 6.5 6.8 7.9 8.3 7.9 -0.9 2.1 3.4 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 28.8128 26.5252 25.9252 22.9352 15.4734 26.3085 31.3874 28.175 25.0335 24.8594 22.5024 26.8272 34.2607 31.4801 29.2808 27.0711 28.2219 30.3251
Actin 0.0152 0.0074 0.0036 0.0398 0.0689 0 0.0077 0.0009 0.0137 0.0253 0 0 0.0049 0 0.0017 0.0037 0 0
Bud 0.0005 0 0.0002 0.0013 0.0004 0.0001 0.0003 0.0002 0.0005 0.0002 0 0 0.0004 0 0.0001 0.0001 0 0
Bud Neck 0.0003 0.0002 0.0001 0.0007 0.0018 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0005 0 0 0 0 0.0001
Bud Periphery 0.0011 0.0001 0.0004 0.0023 0.0005 0.0001 0.0004 0.0003 0.0005 0.0002 0 0.0001 0.0007 0 0.0002 0.0001 0 0.0001
Bud Site 0.001 0.0009 0.0004 0.0074 0.0009 0 0.0003 0.0006 0.0014 0.0004 0 0 0.0086 0 0.0005 0.0002 0 0
Cell Periphery 0.0004 0 0.0005 0.0009 0.0021 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0004 0 0.0002 0 0.0001 0.0001
Cytoplasm 0.0008 0.0001 0.0014 0.003 0.0063 0 0.0021 0.0005 0.0049 0.0002 0 0 0.0004 0 0 0.0006 0 0
Cytoplasmic Foci 0.0064 0.0002 0.0067 0.0008 0.0145 0.0001 0.0074 0.0004 0.0054 0.0021 0 0 0.0122 0 0.0004 0.0115 0 0
Eisosomes 0.0003 0.0001 0.0001 0.0003 0.0013 0 0.0001 0 0.0001 0.0002 0 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0014 0.0001 0.0008 0.0059 0.0078 0 0.0011 0.0005 0.003 0.0005 0 0 0.0001 0 0.0001 0.0012 0 0
Endosome 0.0073 0.0002 0.0061 0.0148 0.0445 0 0.0035 0.0017 0.0057 0.0032 0 0 0.0021 0 0.0001 0.0062 0 0
Golgi 0.0039 0.0003 0.0019 0.0014 0.0095 0 0.001 0.0003 0.0022 0.0047 0 0 0.0012 0 0.0002 0.0024 0 0
Lipid Particles 0.0128 0.0003 0.0082 0.0008 0.0215 0 0.002 0.0002 0.0011 0.0057 0 0 0.0037 0 0.0014 0.0191 0 0
Mitochondria 0.0072 0.0006 0.0101 0.0051 0.0125 0.0012 0.0129 0.0033 0.0055 0.0046 0.0006 0.0004 0.0041 0.0007 0.0077 0.0018 0.0016 0.0004
None 0.0022 0.0001 0.0013 0.0016 0.0426 0 0.0012 0.0002 0.0013 0.0007 0 0 0.0009 0 0.0001 0.0006 0 0
Nuclear Periphery 0.0061 0.0004 0.0034 0.0294 0.0196 0.0002 0.0196 0.0042 0.0127 0.0068 0.0002 0.0001 0.0013 0.0003 0.0002 0.0011 0.0001 0.0001
Nucleolus 0.0794 0.0394 0.0344 0.1723 0.1942 0.1591 0.0467 0.0274 0.0265 0.0571 0.2498 0.1285 0.057 0.0274 0.0207 0.0503 0.1872 0.0947
Nucleus 0.8435 0.9486 0.9126 0.7054 0.4813 0.8376 0.8868 0.955 0.9081 0.8818 0.7487 0.8702 0.8844 0.9714 0.9644 0.8967 0.8102 0.9041
Peroxisomes 0.0053 0.0003 0.0033 0.0002 0.0487 0 0.0024 0.0003 0.0004 0.0016 0 0 0.0045 0 0.0008 0.0024 0 0
Punctate Nuclear 0.0016 0.0004 0.0017 0.0014 0.0136 0 0.0012 0.0022 0.004 0.0034 0.0001 0 0.0086 0 0.0001 0.001 0.0002 0
Vacuole 0.0014 0.0001 0.0011 0.0026 0.0034 0.0006 0.0011 0.0008 0.0013 0.0002 0.0001 0.0002 0.003 0 0.0005 0.0005 0.0001 0.0002
Vacuole Periphery 0.0018 0.0002 0.0018 0.0026 0.0042 0.0005 0.0019 0.0009 0.0016 0.001 0.0002 0.0001 0.0007 0.0001 0.0005 0.0005 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 121.3745 121.6531 134.1878 101.279 114.449 158.0671 147.9364 148.0878 145.8171 111.6543
Translational Efficiency 2.4065 2.5044 1.89 3.5231 2.259 2.3889 2.0354 2.3702 2.3163 2.206

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1474 1576 306 689 511 1083 1642 1117 1985 2659 1948 1806

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1458.11 1894.48 2368.61 2432.45 2111.29 1824.04 2322.00 2403.99 1626.26 1865.79 2329.32 2414.85
Standard Deviation 447.02 527.51 473.78 682.57 577.90 477.90 461.52 589.02 562.06 509.06 463.77 626.51
Intensity Change Log 2 — 0.377702 0.699941 0.738311 — -0.210987 0.137243 0.187306 — 0.059047 0.394094 0.438264

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000488 0.000503 0.000804 0.000754 0.000502 0.000588 0.000615 0.000718 0.000492 0.000538 0.000645 0.000732
Bud Neck 0.001901 0.002366 0.005840 0.009408 0.001602 0.002252 0.006928 0.008133 0.001824 0.002320 0.006757 0.008619
Bud Site 0.000217 0.000675 0.000617 0.000901 0.000205 0.000325 0.000476 0.000672 0.000214 0.000532 0.000498 0.000759
Cell Periphery 0.000066 0.000151 0.000017 0.000147 0.000025 0.000109 0.000075 0.000064 0.000056 0.000134 0.000066 0.000096
Cytoplasm 0.000893 0.000527 0.000012 0.000755 0.000072 0.000540 0.000026 0.000804 0.000682 0.000533 0.000024 0.000786
Cytoplasmic Foci 0.000203 0.000150 0.000288 0.000532 0.000952 0.000272 0.000086 0.001061 0.000396 0.000199 0.000118 0.000859
Eisosomes 0.000302 0.000527 0.000220 0.000088 0.000410 0.000282 0.000254 0.000087 0.000329 0.000427 0.000249 0.000087
Endoplasmic Reticulum 0.000448 0.000366 0.000250 0.000217 0.000210 0.000505 0.000435 0.000495 0.000387 0.000423 0.000406 0.000389
Endosome 0.000016 0.000147 0.000006 0.000144 0.000013 0.000048 0.000007 0.000663 0.000016 0.000107 0.000007 0.000465
Golgi 0.000022 0.000038 0.000048 0.000178 0.000119 0.000213 0.000012 0.000439 0.000047 0.000109 0.000017 0.000340
Lipid Particles 0.000005 0.000453 0.000009 0.000010 0.000071 0.000106 0.000002 0.000152 0.000022 0.000312 0.000003 0.000098
Mitochondria 0.000688 0.000737 0.001786 0.002290 0.000876 0.000902 0.001009 0.001963 0.000736 0.000804 0.001131 0.002088
Mitotic Spindle 0.000026 0.000011 0.000104 0.001334 0.000363 0.003040 0.000004 0.001055 0.000112 0.001245 0.000020 0.001161
None 0.001545 0.000882 0.000401 0.002921 0.000454 0.000876 0.000266 0.000987 0.001264 0.000879 0.000287 0.001725
Nuclear Periphery 0.000029 0.000029 0.000022 0.000160 0.000012 0.000049 0.000024 0.000101 0.000024 0.000037 0.000023 0.000124
Nuclear Periphery Foci 0.000029 0.000040 0.000006 0.000054 0.000007 0.000167 0.000001 0.000089 0.000023 0.000091 0.000002 0.000076
Nucleolus 0.070742 0.052860 0.118328 0.191854 0.044926 0.063494 0.041490 0.173621 0.064096 0.057191 0.053560 0.180577
Nucleus 0.919104* 0.935368* 0.855014* 0.779793* 0.944230* 0.922439* 0.946133* 0.803600* 0.925572* 0.930102* 0.931819* 0.794518*
Peroxisomes 0.002461 0.002112 0.015751 0.006365 0.004448 0.003123 0.001580 0.004269 0.002973 0.002524 0.003806 0.005068
Vacuole 0.000481 0.001626 0.000285 0.000614 0.000289 0.000398 0.000340 0.000499 0.000431 0.001126 0.000331 0.000543
Vacuole Periphery 0.000334 0.000432 0.000193 0.001483 0.000217 0.000272 0.000237 0.000529 0.000304 0.000367 0.000230 0.000893

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.76 -7.88 -8.25 -6.23 1.04 -1.69 -3.91 -5.14 -2.19 -2.74 -1.87 -7.89 -8.88 -5.49 -3.15
Bud Neck -1.59 -9.53 -13.09 -10.93 -6.15 -3.49 -15.42 -14.65 -13.49 -3.72 -2.31 -16.29 -19.04 -16.22 -5.91
Bud Site -1.59 -6.75 -5.96 -0.33 -2.31 -1.52 -4.15 -6.55 -4.95 -3.55 -1.75 -5.81 -8.94 -1.07 -4.57
Cell Periphery -1.74 3.72 -1.53 -0.13 -2.31 -1.92 -3.07 -3.20 0.73 -0.10 -2.26 -0.61 -1.97 0.60 -1.58
Cytoplasm 1.03 1.89 0.17 -0.92 -1.92 -0.93 1.12 -2.77 -0.78 -3.02 0.80 1.90 -0.07 -1.27 -3.41
Cytoplasmic Foci 0.57 -0.91 -2.45 -3.43 -2.20 1.18 1.48 0.06 -1.66 -2.12 1.21 1.66 -1.27 -2.34 -2.65
Eisosomes -1.88 1.93 5.35 3.87 9.82 1.29 1.60 3.41 6.61 7.46 -1.25 1.89 6.33 4.97 8.54
Endoplasmic Reticulum 1.52 4.85 5.90 2.75 1.38 -4.41 -5.56 -1.04 0.14 -0.19 -0.96 -0.55 0.01 0.27 0.14
Endosome -1.34 2.26 -1.13 0.11 -1.22 -2.12 0.76 -1.45 -1.36 -1.47 -1.58 2.14 -1.61 -1.21 -1.64
Golgi -0.94 -3.69 -2.11 -1.85 -1.57 -0.55 1.04 -0.96 -0.40 -1.51 -0.86 1.00 -1.62 -1.01 -1.83
Lipid Particles -1.01 -1.56 -1.11 0.97 -0.67 -0.43 1.02 -0.45 -0.02 -1.28 -1.12 1.06 -1.01 0.84 -1.37
Mitochondria -0.47 -9.49 -11.19 -9.12 -3.26 0.02 -0.47 -3.72 -6.70 -6.48 -0.62 -4.71 -10.65 -10.43 -8.97
Mitotic Spindle 0.84 -1.08 -1.99 -2.01 -1.30 -1.78 1.24 -0.55 1.30 -1.40 -1.98 0.77 -1.87 0.04 -2.09
None 1.62 3.16 -1.08 -1.70 -2.15 -1.88 2.71 -3.15 -0.64 -4.66 1.28 3.68 -0.87 -1.81 -3.26
Nuclear Periphery -0.11 0.12 -4.81 -4.45 -4.79 -2.26 -0.86 -6.04 -3.69 -6.46 -1.34 0.21 -7.62 -6.00 -7.80
Nuclear Periphery Foci -0.13 0.41 -1.02 -0.74 -1.17 -2.70 1.79 -1.18 1.06 -1.29 -2.22 2.22 -1.11 0.43 -1.73
Nucleolus 4.09 -4.89 -12.24 -14.06 -5.47 -3.48 0.45 -16.27 -14.35 -17.74* 1.97 2.20 -19.04 -20.62 -20.14
Nucleus -3.32 5.94 13.45 14.97 5.27 3.27 -0.15 16.33 14.50 18.41 -1.15 -0.98 20.41 21.45 20.82
Peroxisomes 0.99 -7.52 -6.09 -6.81 4.88 1.63 3.47 0.10 -2.45 -5.82 1.37 -2.05 -4.86 -6.77 -2.86
Vacuole -1.81 2.58 -0.53 1.44 -1.25 -1.62 -0.65 -1.37 -0.76 -1.06 -1.83 1.53 -0.80 1.40 -1.55
Vacuole Periphery -1.06 2.92 -1.24 -1.11 -1.40 -1.37 -0.62 -4.33 -3.55 -4.16 -1.05 1.90 -1.71 -1.50 -1.94
Standard name
Human Ortholog
Description High-mobility group (HMG) protein; binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to the chromosomes; functionally redundant with Nhp6Ap; required for transcriptional initiation fidelity of some tRNA genes; homologous to mammalian HMGB1 and HMGB2; NHP6B has a paralog, NHP6A, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Nhp6b

Nhp6b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nhp6b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available