Standard name | |
---|---|
Human Ortholog | |
Description | Protein involved in SRP-independent targeting of substrates to the ER; component of an alternative ER targeting pathway that has partial functional redundancy with the GET pathway; preference for substrates with downstream transmembrane domains; interacts with Snd1p, Env10p/Snd2p, and Sec61p-translocon subunits; can compensate for loss of SRP; role in phosphate transport, interacting with pho88, and in the maturation of secretory proteins |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 9 | 1 | 4 | 2 | 6 | 4 | 8 | 12 | 17 | 1 | 7 | 3 | 8 | 3 | 2 | 1 | 1 | 3 | 5 | 0 | 6 | 10 | 19 |
Bud | 1 | 8 | 0 | 2 | 1 | 4 | 2 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 5 | 26 | 13 | 12 | 19 | 2 | 21 | 14 | 22 | 27 | 2 | 15 | 53 | 101 | 109 | 27 | 38 | 7 | 1 | 2 | 1 | 5 | 14 | 25 |
Cytoplasm | 14 | 50 | 8 | 12 | 16 | 33 | 18 | 51 | 23 | 18 | 12 | 14 | 13 | 29 | 32 | 17 | 11 | 11 | 4 | 27 | 2 | 9 | 4 | 9 |
Endoplasmic Reticulum | 223 | 358 | 106 | 146 | 130 | 117 | 212 | 331 | 174 | 187 | 51 | 105 | 356 | 706 | 594 | 0 | 0 | 2 | 224 | 389 | 115 | 328 | 517 | 554 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Golgi | 7 | 2 | 7 | 10 | 6 | 6 | 20 | 9 | 3 | 7 | 2 | 5 | 9 | 11 | 13 | 3 | 18 | 2 | 1 | 3 | 0 | 8 | 6 | 4 |
Mitochondria | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 2 | 0 | 3 | 2 | 2 | 0 | 3 | 5 | 6 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 3 | 4 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 4 | 2 | 2 | 0 | 1 | 7 | 9 | 7 | 4 | 5 | 0 | 2 | 0 | 2 | 2 | 2 | 0 | 0 | 0 | 13 | 0 | 3 | 1 | 1 |
Unique Cell Count | 240 | 416 | 120 | 173 | 159 | 167 | 258 | 399 | 212 | 231 | 66 | 133 | 378 | 791 | 684 | 51 | 59 | 22 | 242 | 454 | 122 | 371 | 569 | 631 |
Labelled Cell Count | 257 | 456 | 137 | 186 | 175 | 179 | 287 | 425 | 243 | 266 | 73 | 152 | 434 | 857 | 755 | 53 | 68 | 26 | 242 | 454 | 122 | 371 | 569 | 631 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 67.9 | 58.8 | 59.3 | 60.4 | 59.1 | 36.8 | 45.7 | 42.5 | 38.7 | 39.5 | 36.3 | 36.9 | 73.9 | 69.7 | 63.9 | 72.7 | 76.3 | 68.1 | 58.8 | 60.0 | 58.9 |
Std Deviation (1e-4) | 14.9 | 12.2 | 11.3 | 12.9 | 15.3 | 14.7 | 10.6 | 10.1 | 9.5 | 9.0 | 8.4 | 9.2 | 13.9 | 12.8 | 12.6 | 20.0 | 16.0 | 19.6 | 13.4 | 12.8 | 13.0 |
Intensity Change (Log2) | – | – | – | 0.03 | -0.01 | -0.69 | -0.38 | -0.48 | -0.62 | -0.59 | -0.71 | -0.69 | 0.32 | 0.23 | 0.11 | 0.29 | 0.36 | 0.2 | -0.01 | 0.02 | -0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.2 | 0.3 | -3.6 | -0.9 | -3.2 | -0.1 | 0.2 | -1.9 | 0.1 | 0.9 | 0.6 | 1.4 | 6.0 | 7.5 | 0 |
Cytoplasm | 0.1 | 1.0 | 3.1 | 0.1 | 1.9 | 1.3 | 0.4 | 2.4 | 1.1 | -1.5 | 0 | -0.9 | 4.5 | 2.4 | 0 |
Endoplasmic Reticulum | -1.0 | -1.5 | -3.7 | -1.5 | -1.4 | -1.5 | -1.8 | -2.0 | -2.0 | 2.2 | 0.3 | -0.4 | -10.9 | -11.3 | -8.0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | -0.8 | -0.9 | 0.7 | 0 | 0 | 0 | 0 | -0.8 | 0 | 0 | 0 | 0 | 4.5 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 76.1787 | 72.683 | 72.951 | 71.2406 | 71.2472 | 70.5694 | 100.4203 | 93.016 | 91.1449 | 93.3239 | 96.9741 | 91.6893 | 89.0733 | 85.1175 | 82.126 | 81.7429 | 74.4334 | 81.8188 |
Actin | 0.0017 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0019 | 0.0015 | 0.0002 | 0 | 0 | 0.0001 | 0.0049 | 0.0006 | 0.0004 | 0.0012 | 0.0011 | 0.0011 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0007 | 0 |
Bud Neck | 0.0002 | 0.0002 | 0.0008 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0002 | 0.0009 | 0.0007 | 0.0006 | 0.0006 | 0.0021 | 0.0026 | 0.0035 |
Bud Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0002 | 0.0018 | 0.0002 |
Bud Site | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0002 | 0.0005 | 0 | 0 | 0.0018 | 0 |
Cell Periphery | 0.0321 | 0.0598 | 0.0443 | 0.046 | 0.057 | 0.0223 | 0.0378 | 0.076 | 0.0435 | 0.051 | 0.065 | 0.0258 | 0.05 | 0.083 | 0.0395 | 0.0253 | 0.0114 | 0.0146 |
Cytoplasm | 0.0064 | 0.019 | 0.0052 | 0.0015 | 0.0013 | 0.0017 | 0.0087 | 0.0123 | 0.0125 | 0.0056 | 0.0037 | 0.004 | 0.0198 | 0.029 | 0.0209 | 0.0063 | 0.004 | 0.0118 |
Cytoplasmic Foci | 0.0035 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0017 | 0.0003 | 0.0001 | 0.0001 | 0.0015 | 0.0001 |
Eisosomes | 0.0019 | 0.0017 | 0.001 | 0 | 0.0005 | 0.0001 | 0.0003 | 0.0019 | 0.0013 | 0.0003 | 0.0005 | 0.0003 | 0.0054 | 0.0084 | 0.0015 | 0.0007 | 0.0004 | 0.0008 |
Endoplasmic Reticulum | 0.7912 | 0.8567 | 0.896 | 0.9185 | 0.892 | 0.9038 | 0.8715 | 0.8538 | 0.9003 | 0.896 | 0.8604 | 0.9312 | 0.7413 | 0.8017 | 0.8224 | 0.8767 | 0.865 | 0.8493 |
Endosome | 0.0031 | 0.0002 | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0017 | 0.0004 | 0.0005 | 0.0004 | 0.0017 | 0.0006 |
Golgi | 0.0024 | 0.0009 | 0.0006 | 0.0001 | 0.003 | 0.0008 | 0.0003 | 0.0015 | 0.0018 | 0.0004 | 0.0002 | 0.0003 | 0.0018 | 0.0019 | 0.0045 | 0.0032 | 0.0015 | 0.0018 |
Lipid Particles | 0.0071 | 0.0088 | 0.0074 | 0.0021 | 0.0034 | 0.0059 | 0.0042 | 0.0174 | 0.0061 | 0.0051 | 0.006 | 0.004 | 0.0049 | 0.0206 | 0.0074 | 0.0015 | 0.0027 | 0.0021 |
Mitochondria | 0.0009 | 0 | 0.0003 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0006 | 0.0004 | 0.0001 | 0.0001 | 0.0024 | 0.0001 |
None | 0.001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0029 | 0.0002 | 0.0002 | 0.0001 | 0.0014 | 0.0001 |
Nuclear Periphery | 0.1284 | 0.0397 | 0.0315 | 0.0201 | 0.0298 | 0.0546 | 0.0621 | 0.0229 | 0.0251 | 0.0322 | 0.0531 | 0.0277 | 0.1436 | 0.0377 | 0.0865 | 0.0734 | 0.0472 | 0.1043 |
Nucleolus | 0.0007 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0016 | 0.0001 | 0.0001 | 0.0001 | 0.0235 | 0.0001 |
Nucleus | 0.0029 | 0.0004 | 0.0055 | 0.0001 | 0.0002 | 0.0008 | 0.0006 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0006 | 0.0031 | 0.001 | 0.0018 | 0.0024 | 0.0091 | 0.0029 |
Peroxisomes | 0.0024 | 0 | 0 | 0 | 0 | 0 | 0.0014 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0.0004 | 0 |
Punctate Nuclear | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.001 | 0.0001 | 0.0001 | 0.0001 | 0.012 | 0.0002 |
Vacuole | 0.0114 | 0.0115 | 0.0063 | 0.0113 | 0.0118 | 0.0068 | 0.0092 | 0.0111 | 0.0078 | 0.0085 | 0.0101 | 0.0044 | 0.0108 | 0.0124 | 0.0117 | 0.005 | 0.0051 | 0.0041 |
Vacuole Periphery | 0.0021 | 0.0006 | 0.0004 | 0.0001 | 0.0006 | 0.002 | 0.0006 | 0.0002 | 0.0004 | 0.0004 | 0.0004 | 0.0003 | 0.0036 | 0.0008 | 0.0016 | 0.0011 | 0.0028 | 0.0024 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 955.5054 | 828.0689 | 739.679 | 649.9472 | 648.4432 | 797.4345 | 740.5174 | 689.7468 | 706.1414 | 701.7771 |
Translational Efficiency | 1.1768 | 1.1462 | 1.2219 | 1.3983 | 1.4674 | 1.3835 | 1.2841 | 1.3037 | 1.2365 | 1.2878 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1672 | 1038 | 1521 | 109 | 1660 | 1112 | 439 | 1303 | 3332 | 2150 | 1960 | 1412 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 3515.54 | 3706.70 | 5150.34 | 7045.02 | 3276.42 | 4263.69 | 6366.56 | 6018.39 | 3396.41 | 3994.78 | 5422.75 | 6097.64 |
Standard Deviation | 745.01 | 1050.03 | 936.02 | 1237.05 | 683.22 | 806.49 | 914.60 | 1334.55 | 724.82 | 972.72 | 1060.36 | 1355.27 |
Intensity Change Log 2 | — | 0.076389 | 0.550921 | 1.002857 | — | 0.379982 | 0.958394 | 0.877257 | — | 0.230822 | 0.761853 | 0.943632 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000615 | 0.001931 | 0.001081 | 0.001305 | 0.000382 | 0.000595 | 0.000517 | 0.002765 | 0.000499 | 0.001240 | 0.000955 | 0.002652 |
Bud Neck | 0.000400 | 0.002269 | 0.004025 | 0.004537 | 0.000399 | 0.000433 | 0.009343 | 0.006443 | 0.000400 | 0.001320 | 0.005216 | 0.006296 |
Bud Site | 0.000198 | 0.003807 | 0.002412 | 0.003014 | 0.000376 | 0.001167 | 0.001521 | 0.014697 | 0.000286 | 0.002442 | 0.002213 | 0.013795 |
Cell Periphery | 0.007064 | 0.017601 | 0.018325 | 0.113666* | 0.008543 | 0.006551 | 0.047724 | 0.035930 | 0.007801 | 0.011886 | 0.024910 | 0.041931 |
Cytoplasm | 0.000159 | 0.002214 | 0.001220 | 0.000212 | 0.000278 | 0.000259 | 0.000125 | 0.007679 | 0.000218 | 0.001203 | 0.000975 | 0.007102 |
Cytoplasmic Foci | 0.000254 | 0.001432 | 0.000153 | 0.000216 | 0.000287 | 0.000253 | 0.000080 | 0.004218 | 0.000270 | 0.000822 | 0.000137 | 0.003909 |
Eisosomes | 0.001736 | 0.002152 | 0.001179 | 0.006933 | 0.001876 | 0.000978 | 0.007466 | 0.000756 | 0.001806 | 0.001545 | 0.002587 | 0.001233 |
Endoplasmic Reticulum | 0.948418* | 0.779639* | 0.885806* | 0.594980* | 0.956443* | 0.966314* | 0.791827* | 0.591829* | 0.952416* | 0.876189* | 0.864757* | 0.592072* |
Endosome | 0.002795 | 0.012779 | 0.006917 | 0.006825 | 0.003238 | 0.002970 | 0.007295 | 0.024381 | 0.003016 | 0.007706 | 0.007002 | 0.023026 |
Golgi | 0.014032 | 0.045825 | 0.007131 | 0.058417 | 0.012082 | 0.009753 | 0.018589 | 0.038837 | 0.013061 | 0.027168 | 0.009697 | 0.040349 |
Lipid Particles | 0.005300 | 0.054764 | 0.031002 | 0.044618 | 0.004065 | 0.003318 | 0.029680 | 0.165198* | 0.004685 | 0.028156 | 0.030706 | 0.155889* |
Mitochondria | 0.001602 | 0.001541 | 0.000517 | 0.000859 | 0.000546 | 0.000334 | 0.000226 | 0.001837 | 0.001076 | 0.000916 | 0.000452 | 0.001761 |
Mitotic Spindle | 0.000063 | 0.000252 | 0.000187 | 0.000421 | 0.000069 | 0.000046 | 0.000272 | 0.002344 | 0.000066 | 0.000145 | 0.000206 | 0.002195 |
None | 0.000603 | 0.002832 | 0.000308 | 0.000258 | 0.000786 | 0.000865 | 0.000071 | 0.004165 | 0.000694 | 0.001815 | 0.000255 | 0.003863 |
Nuclear Periphery | 0.003304 | 0.004695 | 0.009014 | 0.037775 | 0.002866 | 0.001724 | 0.020922 | 0.007580 | 0.003086 | 0.003158 | 0.011681 | 0.009911 |
Nuclear Periphery Foci | 0.001211 | 0.015855 | 0.005439 | 0.003816 | 0.000864 | 0.000426 | 0.006928 | 0.029971 | 0.001038 | 0.007875 | 0.005773 | 0.027952 |
Nucleolus | 0.000026 | 0.000099 | 0.000015 | 0.000058 | 0.000014 | 0.000007 | 0.000023 | 0.000053 | 0.000020 | 0.000052 | 0.000017 | 0.000053 |
Nucleus | 0.000068 | 0.000512 | 0.000277 | 0.000799 | 0.000055 | 0.000051 | 0.000121 | 0.000871 | 0.000062 | 0.000273 | 0.000242 | 0.000866 |
Peroxisomes | 0.000035 | 0.000046 | 0.000013 | 0.000008 | 0.000038 | 0.000058 | 0.000006 | 0.000141 | 0.000037 | 0.000052 | 0.000012 | 0.000131 |
Vacuole | 0.005528 | 0.035084 | 0.022487 | 0.106202 | 0.004543 | 0.002636 | 0.051719 | 0.053493 | 0.005037 | 0.018302 | 0.029034 | 0.057562 |
Vacuole Periphery | 0.006590 | 0.014671 | 0.002491 | 0.015081 | 0.002249 | 0.001261 | 0.005544 | 0.006815 | 0.004428 | 0.007735 | 0.003175 | 0.007453 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.35 | -1.28 | -1.08 | 0.10 | -0.36 | -0.80 | -0.58 | -4.56 | -3.87 | -3.97 | -1.52 | -1.53 | -4.48 | -2.41 | -2.64 |
Bud Neck | -6.43 | -7.65 | -2.45 | -1.50 | -0.42 | -0.37 | -5.19 | -9.18 | -8.52 | 1.06 | -4.62 | -9.07 | -9.49 | -7.94 | -2.01 |
Bud Site | -4.67 | -3.68 | -1.30 | -0.24 | -0.56 | -1.71 | -3.34 | -8.70 | -8.00 | -7.84 | -4.85 | -4.05 | -8.85 | -7.30 | -7.19 |
Cell Periphery | -6.39 | -8.04 | -7.18 | -6.44 | -6.37 | 1.68 | -9.03 | -11.63 | -12.57 | 2.39 | -4.08 | -11.59 | -13.93 | -11.88 | -6.11 |
Cytoplasm | -4.39 | -2.82 | -0.59 | 3.85 | 2.38 | -0.01 | 0.99 | -7.30 | -7.27 | -7.50 | -3.92 | -2.62 | -7.39 | -6.20 | -6.06 |
Cytoplasmic Foci | -3.60 | 2.22 | 0.31 | 3.53 | -0.49 | 0.20 | 2.05 | -7.63 | -7.60 | -8.13 | -3.11 | 2.22 | -7.73 | -6.26 | -8.01 |
Eisosomes | -0.40 | 1.34 | -3.50 | -2.79 | -3.95 | 2.06 | -3.45 | 2.70 | 1.11 | 4.27 | 0.51 | -1.67 | 1.86 | 0.65 | 3.29 |
Endoplasmic Reticulum | 17.13 | 10.46 | 11.39 | 5.67 | 9.09 | -2.55 | 12.05 | 35.67* | 36.39* | 12.08 | 13.99 | 15.85 | 37.31 | 26.50 | 24.79 |
Endosome | -14.98 | -11.14 | -2.46 | 3.49 | 0.16 | 0.96 | -5.92 | -20.05 | -20.20 | -13.59 | -12.87 | -12.25 | -20.38 | -14.73 | -15.48 |
Golgi | -10.35 | 6.44 | -6.16 | -1.54 | -7.16 | 2.07 | -4.28 | -9.96 | -10.74 | -6.96 | -8.61 | 4.14 | -10.85 | -4.42 | -12.23 |
Lipid Particles | -12.76 | -9.33 | -3.64 | 0.94 | -1.15 | 0.97 | -6.39 | -24.30 | -24.35 | -17.13 | -11.84 | -11.32 | -24.26 | -19.53 | -18.68 |
Mitochondria | 0.11 | 2.04 | 0.25 | 0.18 | -0.74 | 1.51 | 2.48 | -3.47 | -4.09 | -4.39 | 0.52 | 2.02 | -1.99 | -2.43 | -3.53 |
Mitotic Spindle | -2.28 | -2.40 | -7.08 | -1.58 | -2.70 | 1.42 | -2.98 | -4.47 | -4.50 | -4.02 | -2.00 | -3.24 | -4.52 | -4.34 | -4.19 |
None | -2.71 | 2.44 | 1.04 | 2.94 | -0.15 | -0.16 | 2.03 | -3.34 | -3.28 | -4.27 | -2.40 | 2.29 | -3.54 | -2.13 | -4.08 |
Nuclear Periphery | -3.52 | -8.49 | -8.22 | -7.85 | -6.69 | 4.70 | -10.28 | -8.24 | -10.50 | 7.21 | -0.43 | -12.95 | -10.62 | -10.25 | 2.06 |
Nuclear Periphery Foci | -7.59 | -7.55 | -1.94 | 5.20 | 1.36 | 2.30 | -5.05 | -12.79 | -12.95 | -8.96 | -7.23 | -9.38 | -12.75 | -8.76 | -10.15 |
Nucleolus | -2.57 | 2.26 | -2.51 | 1.19 | -3.25 | 1.55 | -2.79 | -3.86 | -4.21 | -3.02 | -2.34 | 0.74 | -3.62 | -0.54 | -3.80 |
Nucleus | -3.20 | -3.28 | -1.11 | -0.72 | -0.87 | -0.14 | -2.99 | -4.68 | -4.66 | -4.37 | -3.16 | -3.65 | -4.76 | -3.77 | -3.74 |
Peroxisomes | -0.75 | 5.15 | 6.33 | 2.88 | 2.03 | -1.37 | 4.30 | -3.87 | -2.89 | -5.31 | -1.54 | 5.73 | -3.93 | -3.11 | -5.07 |
Vacuole | -10.98 | -9.73 | -7.04 | -4.83 | -5.71 | 2.19 | -10.21 | -17.63 | -18.16 | -0.81 | -9.59 | -14.00 | -18.81 | -12.94 | -8.89 |
Vacuole Periphery | -5.43 | 4.26 | -4.05 | -0.22 | -6.48 | 2.73 | -4.42 | -7.42 | -8.63 | -1.33 | -4.38 | 2.14 | -4.24 | 0.31 | -6.83 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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