Standard name
Human Ortholog
Description U1 snRNP A protein; homolog of human U1-A; involved in nuclear mRNA splicing
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.07 0 0.06 0 0.05 0.05 0.05 0.05 0.05 0 0 0.06 0.09 0.16 0.09 0.06 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.27 0.38 0 0 0 0.13 0.3 0.15 0.37 0.33 0.44 0.26 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.91 0.85 0.92 0.94 0.93 0.92 0.82 0.89 0.79 0.82 0.79 0.84 0.93 0.89 0.82 0.85 0.87 0.79 0.78 0.83 0.79 0.84 0.76 0.73
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.2 0.05 0.06 0 0 0 0 0 0.07 0.06 0.12 0.07 0 0 0 0 0 0 0.13 0 0 0.08 0.08 0.08
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0.07 0.06 0 0.08 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud 0 0 0 0 0 0 1 2 3 1 3 1 0 0 0 1 0 0 0 0 0 1 5 2
Bud Neck 0 0 0 0 4 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 1 1 3 10 5 8 6 7 12 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 1 4 5 11 14 19 22 24 16 18 11 7 21 45 80 22 10 21 0 0 0 2 3 5
Endoplasmic Reticulum 0 0 1 0 0 0 0 0 0 1 0 0 1 0 1 3 1 4 0 0 0 0 0 2
Endosome 0 0 0 0 3 2 0 1 0 0 0 0 0 1 3 2 3 3 3 0 5 3 3 7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 3 2 2 0 1 0 0 3
Mitochondria 33 21 2 7 9 44 134 77 111 130 124 125 7 4 0 6 5 9 1 0 1 1 6 7
Nucleus 112 47 104 181 336 317 362 453 238 327 221 406 315 429 413 208 141 167 98 66 96 213 297 290
Nuclear Periphery 0 0 0 0 1 3 4 0 2 4 4 2 1 1 5 1 1 2 0 0 0 0 1 0
Nucleolus 24 3 7 3 2 4 16 13 22 23 35 34 1 8 5 6 3 6 15 2 4 21 29 32
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 0 1 0 0 0 0 0 0
SpindlePole 0 0 1 2 13 4 7 15 5 2 2 7 4 5 8 6 1 0 0 1 1 2 8 5
Vac/Vac Membrane 1 1 1 0 0 1 2 4 2 1 2 5 2 1 2 13 8 8 2 5 6 4 30 32
Unique Cell Count 123 55 113 192 363 346 440 510 303 400 280 483 340 480 506 245 162 212 125 80 122 254 392 400
Labelled Cell Count 171 76 121 205 383 397 558 595 408 514 409 599 352 495 520 270 176 223 125 80 122 254 392 400


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 4.8 6.1 5.9 5.6 4.8 4.5 5.2 4.4 4.6 4.4 4.7 6.5 6.5 6.5 8.8 8.4 8.5 5.9 6.0 7.1
Std Deviation (1e-4) 0.9 1.4 1.3 1.4 1.4 1.3 1.3 1.7 1.4 1.1 1.3 1.0 1.4 1.3 1.6 2.5 2.0 2.8 1.2 1.2 2.3
Intensity Change (Log2) – – – -0.04 -0.12 -0.35 -0.45 -0.22 -0.47 -0.43 -0.49 -0.37 0.08 0.08 0.09 0.53 0.45 0.47 -0.04 -0.03 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8174 1.2414 1.1263 0.7798 0.4039 0.9889 0.4364 1.1184 0.7405 0.3228 0.3891 0.804 0.5589 0.7477 0.4628 0.6022 0.7898 0.6056
Actin 0.019 0 0.0017 0.0058 0.0005 0.0006 0.0278 0 0.0006 0.0086 0.0002 0 0.0137 0 0.0194 0 0.0534 0
Bud 0.0006 0 0.0001 0.0003 0.0004 0 0.0003 0 0.0001 0.0002 0 0 0.0006 0 0.0002 0 0.0007 0
Bud Neck 0.0035 0.0001 0.0048 0.0005 0.0007 0.0007 0.0069 0.0001 0.0005 0.0123 0.0009 0.0012 0.0058 0.0002 0.0003 0 0.0012 0.0008
Bud Periphery 0.0013 0 0.0005 0.0008 0.0024 0 0.0003 0 0.0001 0.0004 0.0001 0 0.0007 0 0.0002 0 0.0019 0
Bud Site 0.0034 0 0.0027 0.0016 0.0011 0.0001 0.0046 0.0001 0.0008 0.005 0.0001 0 0.0025 0.0003 0.0012 0 0.0008 0
Cell Periphery 0.0003 0 0.0001 0.0003 0.001 0 0.0002 0 0 0.0004 0 0 0.0001 0 0.0001 0 0.0002 0
Cytoplasm 0.0042 0.0009 0.001 0.0015 0.0037 0.0003 0.0069 0.0034 0.0002 0.0036 0.0009 0.0001 0.0027 0 0.0018 0 0.0008 0.0001
Cytoplasmic Foci 0.0205 0 0.0024 0.003 0.0075 0.0016 0.0238 0.0001 0.0109 0.0244 0.0071 0 0.0105 0.0004 0.0046 0 0.0001 0
Eisosomes 0.0004 0 0.0001 0.0005 0.0001 0 0.0002 0 0 0.0004 0 0 0.0003 0 0.0002 0 0.0004 0
Endoplasmic Reticulum 0.0023 0 0.0005 0.0025 0.003 0 0.0018 0.0001 0 0.0005 0.0001 0 0.0008 0 0.0008 0 0.0006 0
Endosome 0.0251 0 0.0023 0.0192 0.0044 0.0007 0.0267 0.0001 0.0028 0.0224 0.0007 0 0.0067 0 0.0062 0 0.0013 0
Golgi 0.0042 0 0.0018 0.006 0.0006 0.0005 0.0126 0 0.0019 0.0041 0.0009 0 0.0029 0 0.003 0 0.0022 0
Lipid Particles 0.007 0 0.0107 0.009 0.0056 0.0008 0.0336 0 0.011 0.0118 0.0281 0 0.0123 0.0005 0.0107 0 0.0004 0
Mitochondria 0.0256 0.0001 0.0096 0.0608 0.026 0.0001 0.0035 0.0002 0.004 0.0029 0.0003 0 0.0116 0.0001 0.0015 0.0001 0.0026 0
None 0.0026 0.0081 0.0029 0.0011 0.0117 0.0052 0.0048 0.002 0.0002 0.0032 0.0007 0 0.005 0 0.0092 0 0.0007 0
Nuclear Periphery 0.0339 0.0058 0.0022 0.0125 0.0041 0.0005 0.0067 0.0037 0.0013 0.0038 0.0036 0.0002 0.0035 0.0003 0.0027 0.0002 0.0019 0.0004
Nucleolus 0.0269 0.0127 0.0142 0.0086 0.0568 0.0463 0.0201 0.0163 0.0108 0.0069 0.0574 0.0453 0.0193 0.0288 0.0128 0.0071 0.0239 0.0372
Nucleus 0.7847 0.9568 0.9344 0.857 0.8273 0.9145 0.7597 0.96 0.9298 0.842 0.8533 0.9416 0.8715 0.9665 0.9089 0.9922 0.9047 0.9601
Peroxisomes 0.0035 0 0.0038 0.002 0.0005 0.0017 0.0312 0 0.0154 0.0147 0.0042 0 0.0158 0.0001 0.0071 0 0.0001 0
Punctate Nuclear 0.0242 0.0152 0.003 0.0019 0.0358 0.026 0.0267 0.0136 0.0091 0.0316 0.0412 0.0115 0.0119 0.0023 0.0088 0.0001 0.0015 0.0012
Vacuole 0.004 0 0.0006 0.0026 0.005 0 0.0011 0.0001 0.0003 0.0006 0.0001 0 0.001 0.0002 0.0002 0 0.0003 0
Vacuole Periphery 0.0029 0 0.0004 0.0024 0.0019 0 0.0006 0.0001 0.0001 0.0003 0.0001 0 0.0006 0 0.0002 0 0.0003 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.5312 10.9964 5.7119 11.2724 12.7591 4.9177 10.5625 13.5076 10.1845 12.6219
Translational Efficiency 0.7852 1.0932 1.4003 0.8523 0.9908 3.0442 1.1815 0.9761 1.3308 1.0199

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
129 1179 2125 127 2290 15 714 781 2419 1194 2839 908

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 847.22 811.42 896.78 1045.62 761.02 867.52 990.02 940.66 765.62 812.12 920.23 955.34
Standard Deviation 113.77 144.99 121.25 126.69 103.32 102.22 128.04 131.46 105.70 144.66 129.48 135.77
Intensity Change Log 2 — -0.062288 0.082017 0.303550 — 0.188963 0.379523 0.305739 — 0.062068 0.230459 0.304586

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000935 0.000850 0.000528 0.002174 0.000267 0.005160 0.001100 0.001206 0.000303 0.000905 0.000672 0.001341
Bud Neck 0.011809 0.009789 0.031612 0.014996 0.008558 0.001023 0.015700 0.022185 0.008731 0.009679 0.027610 0.021179
Bud Site 0.012015 0.001953 0.001848 0.003131 0.003631 0.001277 0.001323 0.007494 0.004078 0.001945 0.001716 0.006884
Cell Periphery 0.000343 0.000130 0.000036 0.000121 0.000188 0.000268 0.000062 0.000136 0.000196 0.000131 0.000043 0.000134
Cytoplasm 0.021320 0.007507 0.000329 0.002160 0.005925 0.001615 0.001342 0.015383 0.006746 0.007433 0.000584 0.013534
Cytoplasmic Foci 0.002450 0.002135 0.000014 0.000075 0.000396 0.016979 0.000066 0.000438 0.000506 0.002322 0.000027 0.000388
Eisosomes 0.000068 0.000039 0.000031 0.000051 0.000017 0.000097 0.000041 0.000033 0.000020 0.000040 0.000034 0.000035
Endoplasmic Reticulum 0.005729 0.005682 0.002654 0.011281 0.003120 0.011683 0.005176 0.007959 0.003259 0.005757 0.003288 0.008424
Endosome 0.001165 0.001073 0.000107 0.000401 0.000277 0.001345 0.000209 0.001450 0.000324 0.001076 0.000133 0.001304
Golgi 0.000171 0.000554 0.000080 0.000166 0.000092 0.025058 0.000109 0.000185 0.000096 0.000862 0.000088 0.000183
Lipid Particles 0.001195 0.001113 0.000279 0.000346 0.000143 0.002613 0.000121 0.001092 0.000199 0.001132 0.000239 0.000987
Mitochondria 0.001140 0.002478 0.001460 0.001976 0.001729 0.004118 0.001140 0.002646 0.001697 0.002499 0.001380 0.002552
Mitotic Spindle 0.000076 0.003249 0.000798 0.003181 0.001439 0.001110 0.000379 0.014970 0.001367 0.003222 0.000693 0.013321
None 0.007960 0.014251 0.001009 0.003656 0.005897 0.019915 0.002592 0.005776 0.006007 0.014322 0.001407 0.005479
Nuclear Periphery 0.000188 0.000493 0.000362 0.000889 0.000595 0.000211 0.000308 0.001482 0.000573 0.000490 0.000349 0.001399
Nuclear Periphery Foci 0.001045 0.001021 0.000048 0.000385 0.000249 0.000578 0.000185 0.000967 0.000291 0.001015 0.000082 0.000885
Nucleolus 0.046154 0.054518 0.032659 0.012944 0.025105 0.027890 0.027213 0.010326 0.026228 0.054184 0.031289 0.010692
Nucleus 0.877703* 0.890210* 0.925412* 0.935976* 0.940950* 0.861265* 0.941080* 0.900437* 0.937577* 0.889846* 0.929352* 0.905408*
Peroxisomes 0.001262 0.001261 0.000173 0.000494 0.000185 0.016577 0.000435 0.000313 0.000242 0.001453 0.000239 0.000339
Vacuole 0.006855 0.001189 0.000410 0.005391 0.000971 0.000951 0.001288 0.005212 0.001285 0.001186 0.000631 0.005237
Vacuole Periphery 0.000417 0.000505 0.000150 0.000206 0.000266 0.000265 0.000131 0.000309 0.000274 0.000502 0.000145 0.000294

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.29 2.27 -6.59 -7.56 -9.63 -1.44 -16.61 -15.61 0.98 -3.59 -6.31 -13.99 -17.75 -7.40 -11.90
Bud Neck 0.31 -4.37 -1.59 -4.76 6.03 10.48 -6.92 -10.10 -14.26 -4.39 -0.98 -18.85 -10.56 -9.78 1.53
Bud Site 2.05 2.07 1.50 -2.95 -2.95 2.47 2.31 -2.51 -4.37 -4.35 2.39 2.57 -2.16 -4.27 -4.42
Cell Periphery 2.05 2.97 1.87 -0.61 -4.94 -0.67 3.38 1.19 1.10 -6.78 1.72 4.42 1.47 -0.66 -10.22
Cytoplasm 1.52 2.38 2.01 2.66 -3.52 4.34 5.92 -2.95 -4.77 -5.06 -0.54 7.76 -2.30 -1.74 -5.65
Cytoplasmic Foci 0.10 2.83 2.70 3.36 -2.60 -0.99 3.75 0.66 0.99 -2.18 -2.89 5.06 1.67 3.23 -2.66
Eisosomes 2.97 3.87 -0.37 -4.73 -6.22 -2.15 -14.99 -13.61 1.19 -1.25 -7.45 -13.76 -14.23 -4.14 -7.51
Endoplasmic Reticulum -0.03 3.10 -4.02 -4.79 -7.70 -1.43 -8.57 -14.07 0.01 -8.42 -5.18 -1.79 -15.18 -8.39 -15.01
Endosome 0.16 2.11 1.04 1.82 -6.50 -1.00 0.44 -3.29 -0.03 -3.34 -3.41 4.52 -3.28 -0.39 -3.93
Golgi -3.79 3.08 -1.62 3.23 -4.95 -1.00 -2.79 -3.13 1.00 -1.97 -2.42 -0.56 -3.49 2.05 -3.57
Lipid Particles 0.13 2.23 1.90 1.92 -1.06 -1.30 -0.26 -2.36 0.91 -2.34 -2.80 -0.07 -2.30 0.85 -1.95
Mitochondria -3.00 -2.40 -5.73 -0.02 -3.01 -1.13 1.00 -2.19 0.54 -4.62 -1.35 0.21 -2.32 -0.78 -3.82
Mitotic Spindle -2.66 -2.46 -2.66 -0.80 -1.98 0.33 0.35 -4.80 -4.77 -4.89 -1.51 0.17 -4.96 -4.07 -5.02
None -2.24 3.33 2.00 5.22 -2.84 -1.06 5.27 1.17 1.13 -3.18 -4.32 8.40 1.66 4.96 -5.51
Nuclear Periphery -4.38 -4.40 -5.56 -4.33 -4.60 2.78 1.82 -10.63 -12.31 -12.97 0.88 1.58 -11.08 -12.56 -13.50
Nuclear Periphery Foci -0.09 2.25 1.33 2.91 -3.24 -0.69 -0.54 -2.51 -1.42 -2.35 -3.94 2.79 -2.46 -0.52 -2.93
Nucleolus -1.24 1.29 4.79 10.21 6.78 -0.34 -0.90 7.41 2.27 6.59 -10.34 -4.82 8.33 15.66 12.46
Nucleus -0.63 -2.18 -1.35 -1.33 0.83 1.20 1.74 9.00 -0.19 7.95 8.65 5.35 8.77 1.12 6.28
Peroxisomes -0.23 5.43 3.76 3.72 -3.25 -1.08 -6.09 -3.89 1.07 1.90 -4.43 -0.53 -3.09 3.92 -3.28
Vacuole 2.53 2.86 -2.19 -3.07 -3.17 0.15 -2.64 -7.22 -7.00 -6.80 0.30 1.88 -7.59 -7.65 -7.89
Vacuole Periphery -0.52 1.96 1.46 3.33 -3.10 0.05 3.34 -0.19 -0.15 -3.32 -2.56 3.39 0.11 2.63 -3.31
Standard name
Human Ortholog
Description U1 snRNP A protein; homolog of human U1-A; involved in nuclear mRNA splicing
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Mud1

Mud1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mud1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available