Standard name | |
---|---|
Human Ortholog | |
Description | Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 10 | 7 | 3 | 3 | 7 | 1 | 1 | 2 | 2 | 2 | 0 | 0 | 8 | 6 | 8 | 8 | 12 | 1 | 6 | 4 | 0 | 7 | 16 |
Bud | 4 | 8 | 4 | 8 | 9 | 28 | 46 | 50 | 33 | 43 | 30 | 46 | 2 | 3 | 4 | 4 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 3 | 0 | 2 | 4 | 19 | 3 | 16 | 5 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 2 | 0 | 4 | 1 | 2 | 0 | 1 | 1 | 2 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 20 | 10 | 14 | 18 | 18 | 41 | 28 | 21 | 23 | 23 | 27 | 9 | 26 | 51 | 2 | 3 | 11 | 0 | 5 | 0 | 2 | 2 | 2 |
Endoplasmic Reticulum | 1 | 18 | 12 | 1 | 12 | 9 | 3 | 3 | 1 | 7 | 0 | 1 | 4 | 24 | 10 | 3 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 0 |
Endosome | 24 | 2 | 0 | 2 | 10 | 9 | 35 | 13 | 0 | 1 | 0 | 0 | 4 | 9 | 2 | 25 | 1 | 14 | 27 | 24 | 29 | 10 | 28 | 36 |
Golgi | 4 | 19 | 0 | 1 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 9 | 22 | 8 | 59 | 30 | 39 | 114 | 67 | 47 | 30 | 81 | 167 |
Mitochondria | 128 | 107 | 128 | 167 | 228 | 280 | 472 | 519 | 283 | 533 | 255 | 421 | 40 | 84 | 127 | 11 | 8 | 10 | 11 | 43 | 39 | 8 | 38 | 29 |
Nucleus | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 3 | 0 | 0 | 1 | 0 | 1 | 6 | 8 | 3 | 4 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 4 | 0 | 0 | 2 | 13 | 8 | 31 | 42 | 6 | 15 | 6 | 7 | 0 | 0 | 0 | 3 | 0 | 0 | 8 | 3 | 12 | 0 | 3 | 8 |
SpindlePole | 2 | 0 | 1 | 6 | 14 | 9 | 47 | 61 | 14 | 23 | 9 | 21 | 0 | 0 | 1 | 4 | 3 | 6 | 0 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 9 | 11 | 2 | 4 | 6 | 3 | 9 | 8 | 2 | 5 | 4 | 6 | 0 | 5 | 3 | 5 | 2 | 3 | 2 | 7 | 3 | 1 | 3 | 2 |
Unique Cell Count | 149 | 150 | 133 | 179 | 270 | 310 | 550 | 584 | 308 | 564 | 279 | 453 | 54 | 151 | 175 | 102 | 40 | 75 | 168 | 166 | 139 | 56 | 173 | 268 |
Labelled Cell Count | 180 | 199 | 164 | 213 | 316 | 376 | 699 | 758 | 371 | 676 | 339 | 543 | 69 | 182 | 213 | 127 | 57 | 101 | 168 | 166 | 139 | 56 | 173 | 268 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.1 | 6.0 | 4.4 | 4.5 | 4.6 | 4.3 | 4.3 | 4.1 | 3.9 | 3.7 | 3.8 | 3.3 | 6.6 | 6.5 | 5.6 | 8.6 | 10.2 | 9.7 | 6.1 | 6.6 | 6.9 |
Std Deviation (1e-4) | 0.9 | 1.4 | 0.5 | 0.8 | 1.1 | 0.9 | 1.2 | 1.0 | 1.5 | 0.9 | 1.4 | 0.8 | 1.1 | 1.0 | 1.0 | 1.1 | 1.8 | 1.3 | 0.9 | 1.2 | 1.1 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.05 | -0.03 | -0.05 | -0.1 | -0.19 | -0.26 | -0.22 | -0.41 | 0.59 | 0.55 | 0.35 | 0.96 | 1.21 | 1.14 | 0.46 | 0.58 | 0.64 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.8 | 0.8 | 0 | 2.6 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.1 | -0.3 | -0.7 | 0 | -1.3 | -0.3 | -1.7 | 0.3 | -0.6 | 1.9 | 2.5 | 4.7 | -1.9 | 0 | 1.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | -1.1 | -1.9 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.0 | 0 | 5.2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.6 | 0 | 10.1 | 11.0 | 9.2 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -4.5 | -7.9 | -5.5 | -13.2 | -10.3 | -12.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.2546 | 2.8569 | 2.3446 | 2.4565 | 2.2942 | 2.7284 | 2.2039 | 3.5756 | 3.504 | 3.1369 | 3.1312 | 3.5733 | 1.0327 | 1.2853 | 1.2184 | 0.9425 | 1.2257 | 0.6189 |
Actin | 0.1057 | 0.0428 | 0.0494 | 0.0018 | 0.0782 | 0.0685 | 0.0686 | 0.0318 | 0.0258 | 0.0714 | 0.0179 | 0.0107 | 0.0708 | 0.0081 | 0.0139 | 0.0006 | 0.0087 | 0.0084 |
Bud | 0.0008 | 0.0009 | 0.0006 | 0.0012 | 0.0008 | 0.0003 | 0.0011 | 0.0003 | 0.0008 | 0.001 | 0.0005 | 0.0002 | 0.0007 | 0.0009 | 0.0005 | 0 | 0.0001 | 0.0002 |
Bud Neck | 0.002 | 0.0013 | 0.001 | 0.0027 | 0.0012 | 0.0025 | 0.0007 | 0.0005 | 0.0003 | 0.0014 | 0.0006 | 0.0006 | 0.0009 | 0.0008 | 0.0003 | 0.0004 | 0.0005 | 0.0005 |
Bud Periphery | 0.0011 | 0.0011 | 0.0004 | 0.0014 | 0.0007 | 0.0004 | 0.0012 | 0.0001 | 0.0008 | 0.0009 | 0.0004 | 0.0002 | 0.0009 | 0.0011 | 0.0005 | 0 | 0.0001 | 0.0002 |
Bud Site | 0.0041 | 0.0153 | 0.0041 | 0.0157 | 0.0038 | 0.0013 | 0.0064 | 0.008 | 0.0006 | 0.0006 | 0.0017 | 0.0003 | 0.0022 | 0.0047 | 0.0025 | 0.0001 | 0.0004 | 0.0002 |
Cell Periphery | 0.0008 | 0.001 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0005 | 0 | 0 | 0.0001 |
Cytoplasm | 0.0102 | 0.0263 | 0.0056 | 0.0057 | 0.0015 | 0.0091 | 0.0105 | 0.0213 | 0.0043 | 0.0061 | 0.009 | 0.006 | 0.0337 | 0.0048 | 0.0213 | 0.0373 | 0.0035 | 0.0054 |
Cytoplasmic Foci | 0.1176 | 0.1508 | 0.1268 | 0.1527 | 0.1193 | 0.1245 | 0.1624 | 0.1791 | 0.1089 | 0.0927 | 0.1312 | 0.1072 | 0.0884 | 0.2188 | 0.156 | 0.0656 | 0.1804 | 0.1048 |
Eisosomes | 0.0007 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0.001 | 0.0002 | 0.0003 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0028 | 0.006 | 0.0018 | 0.0009 | 0.0005 | 0.0121 | 0.0064 | 0.0027 | 0.0017 | 0.0008 | 0.0006 | 0.0022 | 0.0049 | 0.0005 | 0.018 | 0.0019 | 0.0004 | 0.0026 |
Endosome | 0.1731 | 0.1918 | 0.2276 | 0.2522 | 0.2145 | 0.1625 | 0.24 | 0.272 | 0.2801 | 0.3049 | 0.2668 | 0.1821 | 0.175 | 0.1341 | 0.2187 | 0.2323 | 0.3339 | 0.2421 |
Golgi | 0.3923 | 0.3946 | 0.4898 | 0.4738 | 0.523 | 0.4887 | 0.3731 | 0.4488 | 0.5447 | 0.4666 | 0.5323 | 0.6308 | 0.2747 | 0.4367 | 0.3603 | 0.5796 | 0.3005 | 0.4622 |
Lipid Particles | 0.0379 | 0.0134 | 0.0167 | 0.0076 | 0.0172 | 0.046 | 0.0376 | 0.0064 | 0.0089 | 0.0085 | 0.0046 | 0.0183 | 0.1007 | 0.0211 | 0.027 | 0.0051 | 0.0214 | 0.052 |
Mitochondria | 0.0247 | 0.0931 | 0.0338 | 0.0067 | 0.0155 | 0.0432 | 0.0163 | 0.0036 | 0.011 | 0.0256 | 0.0181 | 0.0075 | 0.0386 | 0.1135 | 0.0403 | 0.0046 | 0.0289 | 0.0534 |
None | 0.0613 | 0.0256 | 0.0008 | 0.0082 | 0.0001 | 0.0011 | 0.0072 | 0.0004 | 0.0002 | 0.0065 | 0.0002 | 0.0019 | 0.1365 | 0.0023 | 0.0438 | 0.0671 | 0.0003 | 0.003 |
Nuclear Periphery | 0.0017 | 0.001 | 0.0004 | 0.0002 | 0.0001 | 0.0014 | 0.0104 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0142 | 0.0001 | 0.0049 | 0.0004 | 0.0001 | 0.0006 |
Nucleolus | 0.0018 | 0.0003 | 0 | 0.0013 | 0 | 0.0001 | 0.0004 | 0 | 0 | 0.0007 | 0 | 0 | 0.0039 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0002 |
Nucleus | 0.0019 | 0.0006 | 0.0001 | 0.0006 | 0 | 0.0002 | 0.0027 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0136 | 0.0002 | 0.0015 | 0.0003 | 0.0001 | 0.0002 |
Peroxisomes | 0.0281 | 0.0276 | 0.0375 | 0.0056 | 0.0216 | 0.0329 | 0.0428 | 0.0217 | 0.0081 | 0.0074 | 0.012 | 0.0295 | 0.0158 | 0.0489 | 0.0654 | 0.0018 | 0.1162 | 0.0532 |
Punctate Nuclear | 0.0179 | 0.0006 | 0.0004 | 0.002 | 0.0003 | 0.0009 | 0.0049 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0136 | 0.0003 | 0.0008 | 0.0007 | 0.0004 | 0.0005 |
Vacuole | 0.0106 | 0.0038 | 0.0016 | 0.0542 | 0.0006 | 0.0016 | 0.0029 | 0.0014 | 0.0015 | 0.0017 | 0.0018 | 0.0009 | 0.0058 | 0.0016 | 0.0176 | 0.0009 | 0.0025 | 0.0076 |
Vacuole Periphery | 0.0028 | 0.0016 | 0.0014 | 0.0048 | 0.0008 | 0.002 | 0.0036 | 0.0012 | 0.0017 | 0.0021 | 0.0016 | 0.0011 | 0.0035 | 0.0008 | 0.0055 | 0.0012 | 0.0013 | 0.0027 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 25.7122 | 21.235 | 15.7573 | 19.1133 | 21.4913 | 16.1753 | 27.8411 | 24.1255 | 18.1627 | 27.6034 |
Translational Efficiency | 0.6673 | 0.8051 | 0.7427 | 0.5499 | 0.6395 | 0.726 | 0.5247 | 0.5224 | 0.5813 | 0.5005 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
113 | 1251 | 1127 | 1297 | 784 | 1386 | 288 | 2471 | 897 | 2637 | 1415 | 3768 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 699.92 | 863.45 | 1089.50 | 964.86 | 798.92 | 784.06 | 1037.85 | 938.43 | 786.45 | 821.72 | 1078.99 | 947.53 |
Standard Deviation | 82.80 | 105.04 | 125.95 | 124.62 | 86.85 | 89.27 | 110.89 | 119.63 | 92.39 | 104.85 | 124.78 | 122.02 |
Intensity Change Log 2 | — | 0.302923 | 0.638404 | 0.463130 | — | -0.027087 | 0.377475 | 0.232198 | — | 0.136441 | 0.505211 | 0.344649 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.022856 | 0.002046 | 0.010440 | 0.006589 | 0.002930 | 0.001238 | 0.008541 | 0.016622 | 0.005440 | 0.001621 | 0.010053 | 0.013168 |
Bud Neck | 0.001926 | 0.000245 | 0.000858 | 0.001108 | 0.000283 | 0.000080 | 0.000729 | 0.000281 | 0.000490 | 0.000158 | 0.000832 | 0.000566 |
Bud Site | 0.006353 | 0.000813 | 0.011678 | 0.011136 | 0.001852 | 0.000676 | 0.004561 | 0.004214 | 0.002419 | 0.000741 | 0.010229 | 0.006597 |
Cell Periphery | 0.000278 | 0.000043 | 0.000075 | 0.000035 | 0.000123 | 0.000025 | 0.000056 | 0.000033 | 0.000142 | 0.000034 | 0.000071 | 0.000034 |
Cytoplasm | 0.008105 | 0.000647 | 0.006628 | 0.009085 | 0.003535 | 0.000362 | 0.001879 | 0.006627 | 0.004110 | 0.000498 | 0.005662 | 0.007473 |
Cytoplasmic Foci | 0.186463* | 0.228325* | 0.396920* | 0.373713* | 0.188905* | 0.180830* | 0.387787* | 0.252581* | 0.188598* | 0.203361* | 0.395061* | 0.294277* |
Eisosomes | 0.000544 | 0.000042 | 0.000043 | 0.000036 | 0.000130 | 0.000042 | 0.000051 | 0.000090 | 0.000182 | 0.000042 | 0.000045 | 0.000071 |
Endoplasmic Reticulum | 0.000811 | 0.000183 | 0.004517 | 0.000942 | 0.000527 | 0.000130 | 0.001162 | 0.000260 | 0.000563 | 0.000155 | 0.003834 | 0.000495 |
Endosome | 0.132034 | 0.134659 | 0.120825 | 0.256129* | 0.117187 | 0.138363 | 0.220577* | 0.227993* | 0.119057 | 0.136606 | 0.141128 | 0.237678* |
Golgi | 0.413628* | 0.608419* | 0.317149* | 0.225379* | 0.629023* | 0.645256* | 0.246609* | 0.436368* | 0.601889* | 0.627780* | 0.302792* | 0.363743* |
Lipid Particles | 0.036927 | 0.006740 | 0.016610 | 0.006202 | 0.006060 | 0.005868 | 0.044723 | 0.004205 | 0.009949 | 0.006282 | 0.022332 | 0.004892 |
Mitochondria | 0.045728 | 0.007945 | 0.001213 | 0.004553 | 0.028244 | 0.016657 | 0.000909 | 0.008691 | 0.030447 | 0.012524 | 0.001151 | 0.007267 |
Mitotic Spindle | 0.018743 | 0.000833 | 0.072054 | 0.041555 | 0.001037 | 0.000842 | 0.034172 | 0.012135 | 0.003268 | 0.000837 | 0.064344 | 0.022262 |
None | 0.000201 | 0.000013 | 0.000019 | 0.000016 | 0.000040 | 0.000014 | 0.000020 | 0.000013 | 0.000060 | 0.000014 | 0.000019 | 0.000014 |
Nuclear Periphery | 0.000134 | 0.000011 | 0.000245 | 0.000260 | 0.000021 | 0.000016 | 0.000193 | 0.000037 | 0.000035 | 0.000014 | 0.000234 | 0.000114 |
Nuclear Periphery Foci | 0.000276 | 0.000109 | 0.005504 | 0.000730 | 0.000089 | 0.000081 | 0.005205 | 0.000229 | 0.000113 | 0.000094 | 0.005443 | 0.000402 |
Nucleolus | 0.000611 | 0.000025 | 0.000018 | 0.000052 | 0.000047 | 0.000018 | 0.000256 | 0.000010 | 0.000118 | 0.000021 | 0.000066 | 0.000025 |
Nucleus | 0.006483 | 0.000803 | 0.000172 | 0.000184 | 0.000087 | 0.000019 | 0.000287 | 0.000042 | 0.000893 | 0.000391 | 0.000196 | 0.000091 |
Peroxisomes | 0.114009* | 0.003864 | 0.005830 | 0.020297 | 0.010589 | 0.005630 | 0.022940 | 0.012808 | 0.023617 | 0.004792 | 0.009313 | 0.015386 |
Vacuole | 0.002327 | 0.001854 | 0.026111 | 0.037254 | 0.004046 | 0.001277 | 0.016607 | 0.013196 | 0.003829 | 0.001550 | 0.024177 | 0.021477 |
Vacuole Periphery | 0.001565 | 0.002381 | 0.003088 | 0.004744 | 0.005246 | 0.002578 | 0.002737 | 0.003563 | 0.004782 | 0.002485 | 0.003017 | 0.003969 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 3.68 | 2.10 | 2.82 | -3.77 | 2.44 | 1.57 | -2.38 | -7.32 | -9.41 | -3.03 | 3.22 | -3.09 | -4.88 | -10.04 | -1.91 |
Bud Neck | 2.91 | 1.82 | 1.21 | -5.25 | -2.04 | 3.80 | -3.70 | -0.56 | -5.64 | 3.57 | 3.22 | -3.38 | -1.38 | -5.88 | 2.60 |
Bud Site | 2.73 | -2.34 | -2.20 | -8.55 | 0.27 | 2.64 | -3.13 | -4.67 | -6.31 | 0.23 | 4.02 | -7.01 | -7.03 | -10.68 | 3.02 |
Cell Periphery | 1.73 | 1.47 | 1.79 | 0.94 | 6.99 | 3.00 | 2.01 | 2.76 | -1.97 | 3.95 | 3.27 | 2.04 | 3.27 | -0.15 | 8.28 |
Cytoplasm | 2.56 | 0.23 | -0.66 | -8.48 | -1.80 | 6.06 | 2.37 | -4.31 | -9.32 | -6.38 | 6.04 | -2.14 | -5.05 | -12.43 | -1.93 |
Cytoplasmic Foci | -3.30 | -15.65 | -14.06 | -20.66 | 2.92 | 1.44 | -16.41 | -11.49 | -14.83 | 11.48 | -2.96 | -31.13 | -20.43 | -21.91 | 16.82 |
Eisosomes | 3.94 | 3.92 | 3.98 | 1.20 | 1.94 | 6.44 | 5.44 | 2.31 | -4.36 | -3.20 | 6.88 | 6.67 | 5.13 | -4.03 | -3.46 |
Endoplasmic Reticulum | 2.05 | -5.59 | -0.20 | -2.27 | 5.43 | 3.15 | -2.51 | 1.88 | -2.46 | 3.87 | 3.53 | -6.75 | 0.38 | -2.87 | 6.86 |
Endosome | -0.25 | 0.92 | -9.39 | -18.88 | -22.08 | -4.14 | -9.66 | -20.95 | -17.90 | -0.65 | -4.05 | -4.53 | -24.76 | -26.01 | -21.91 |
Golgi | -7.93 | 3.97 | 7.75 | 46.42* | 10.14 | -1.72 | 26.80 | 21.04 | 27.95 | -14.33 | -2.98 | 30.72 | 27.22 | 45.40* | -8.21 |
Lipid Particles | 4.33 | 2.81 | 4.42 | 0.17 | 7.38 | 0.14 | -5.00 | 1.29 | 1.50 | 5.25 | 2.35 | -5.55 | 3.37 | 1.35 | 9.15 |
Mitochondria | 3.16 | 3.74 | 3.46 | 2.14 | -2.73 | 3.41 | 9.66 | 6.64 | 3.79 | -8.65 | 5.77 | 10.10 | 7.83 | 3.93 | -8.14 |
Mitotic Spindle | 2.24 | -5.80 | -2.83 | -11.36 | 4.75 | 0.68 | -4.87 | -8.65 | -8.86 | 3.13 | 2.32 | -14.64 | -10.58 | -14.20 | 9.53 |
None | 1.61 | 1.55 | 1.59 | -0.60 | 0.90 | 3.46 | 1.24 | 3.45 | 0.01 | 0.63 | 2.83 | 2.36 | 2.76 | -0.42 | 1.62 |
Nuclear Periphery | 1.33 | -1.35 | -0.72 | -1.71 | 0.05 | 0.69 | -4.90 | -2.52 | -3.79 | 4.40 | 1.64 | -8.20 | -1.54 | -2.01 | 2.49 |
Nuclear Periphery Foci | 1.86 | -6.06 | -3.74 | -5.85 | 5.49 | 0.26 | -3.30 | -3.07 | -3.12 | 3.20 | 0.62 | -7.06 | -6.04 | -6.34 | 6.66 |
Nucleolus | 1.59 | 1.60 | 1.50 | -1.85 | -2.48 | 1.43 | -1.35 | 1.80 | 0.85 | 1.47 | 1.92 | 0.13 | 1.80 | -0.79 | 1.34 |
Nucleus | 1.38 | 1.53 | 1.52 | 0.71 | -0.27 | 3.00 | -1.41 | 1.26 | -2.49 | 1.62 | 0.86 | 1.28 | 1.49 | 0.72 | 2.10 |
Peroxisomes | 7.00 | 6.87 | 5.89 | -8.57 | -7.09 | 2.98 | -2.87 | -1.34 | -5.16 | 2.37 | 7.09 | 4.97 | 2.86 | -9.66 | -4.07 |
Vacuole | 0.71 | -12.24 | -13.17 | -13.94 | -4.21 | 3.41 | -4.69 | -7.84 | -12.02 | 0.83 | 3.18 | -12.60 | -14.23 | -17.98 | 0.73 |
Vacuole Periphery | -1.17 | -2.33 | -3.67 | -3.38 | -2.41 | 3.09 | 2.79 | 2.02 | -2.22 | -1.61 | 3.12 | 2.39 | 1.13 | -4.02 | -2.57 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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