Standard name | |
---|---|
Human Ortholog | |
Description | Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation; also involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing (RUP) |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 2 | 0 | 0 | 1 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 10 | 10 | 5 | 4 | 5 | 9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 1 | 0 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 3 | 0 | 2 | 3 | 1 | 0 | 0 | 0 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 164 | 331 | 89 | 53 | 71 | 87 | 105 | 113 | 83 | 61 | 72 | 56 | 124 | 133 | 134 | 74 | 87 | 76 | 145 | 276 | 85 | 43 | 46 | 40 |
Endoplasmic Reticulum | 6 | 18 | 6 | 9 | 8 | 12 | 19 | 26 | 4 | 7 | 4 | 1 | 6 | 8 | 1 | 5 | 9 | 9 | 22 | 45 | 30 | 3 | 6 | 5 |
Endosome | 0 | 1 | 0 | 1 | 0 | 0 | 3 | 1 | 1 | 0 | 0 | 6 | 2 | 1 | 0 | 3 | 3 | 1 | 5 | 10 | 2 | 1 | 6 | 10 |
Golgi | 1 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 7 | 13 | 7 | 1 | 1 | 1 | 0 | 0 | 1 |
Mitochondria | 1 | 0 | 0 | 0 | 0 | 0 | 7 | 8 | 9 | 11 | 51 | 21 | 0 | 2 | 0 | 7 | 1 | 0 | 2 | 2 | 4 | 0 | 3 | 1 |
Nucleus | 18 | 36 | 18 | 37 | 32 | 34 | 63 | 79 | 35 | 46 | 36 | 40 | 9 | 24 | 26 | 27 | 15 | 18 | 55 | 86 | 43 | 39 | 45 | 88 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 2 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 17 | 20 | 8 | 1 | 3 | 4 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 4 | 1 | 1 | 5 | 6 |
Vac/Vac Membrane | 117 | 215 | 124 | 96 | 116 | 108 | 203 | 199 | 150 | 131 | 94 | 136 | 128 | 121 | 162 | 318 | 293 | 251 | 36 | 90 | 50 | 14 | 24 | 48 |
Unique Cell Count | 260 | 506 | 201 | 164 | 199 | 212 | 348 | 364 | 249 | 229 | 222 | 235 | 230 | 218 | 262 | 403 | 376 | 317 | 314 | 561 | 240 | 113 | 153 | 228 |
Labelled Cell Count | 307 | 604 | 238 | 196 | 228 | 244 | 406 | 431 | 287 | 263 | 264 | 266 | 270 | 289 | 326 | 446 | 428 | 363 | 314 | 561 | 240 | 113 | 153 | 228 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.9 | 12.7 | 10.9 | 10.8 | 11.5 | 10.1 | 9.8 | 9.8 | 9.3 | 8.9 | 8.4 | 8.3 | 12.1 | 11.5 | 11.5 | 16.2 | 15.7 | 15.8 | 11.7 | 11.2 | 12.7 |
Std Deviation (1e-4) | 1.5 | 1.8 | 1.7 | 1.6 | 1.9 | 1.9 | 1.7 | 1.6 | 2.0 | 1.8 | 1.8 | 1.6 | 2.3 | 1.9 | 1.8 | 4.2 | 4.7 | 4.5 | 2.4 | 2.2 | 2.3 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.08 | -0.12 | -0.16 | -0.15 | -0.24 | -0.29 | -0.37 | -0.39 | 0.15 | 0.08 | 0.08 | 0.57 | 0.53 | 0.53 | 0.11 | 0.04 | 0.22 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -2.3 | -1.8 | -0.7 | -3.3 | -3.1 | -2.4 | -3.8 | -2.5 | -4.5 | 2.0 | 3.4 | 1.5 | -6.8 | -5.3 | -4.8 |
Endoplasmic Reticulum | 1.2 | 0.6 | 1.3 | 1.3 | 2.0 | 0 | 0 | 0 | 0 | -0.2 | 0.4 | 0 | 0 | -0.4 | -0.1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 7.2 | 4.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 3.6 | 2.2 | 2.2 | 2.9 | 3.8 | 1.7 | 3.2 | 2.2 | 2.5 | -2.2 | 0.7 | 0.4 | -1.0 | -2.4 | -1.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.6 | -0.7 | -2.2 | -0.8 | -1.6 | -0.3 | -0.9 | -4.0 | -0.8 | -1.3 | -1.3 | 0 | 4.5 | 4.2 | 4.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.0176 | 7.6376 | 7.6939 | 6.8928 | 6.9662 | 7.2927 | 6.6044 | 7.3462 | 7.1039 | 6.4267 | 6.3463 | 6.7859 | 6.8354 | 8.4222 | 8.0438 | 8.3522 | 8.8784 | 7.6245 |
Actin | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.019 | 0.0004 | 0.0009 | 0.0009 | 0.0128 | 0.0013 | 0.0213 | 0.0005 | 0.0127 | 0.0106 | 0.0002 | 0.0002 |
Bud | 0 | 0.0001 | 0.0018 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0058 | 0.0001 | 0.0001 | 0.001 | 0.0001 | 0.0001 |
Bud Neck | 0.0005 | 0.0005 | 0.0009 | 0.0011 | 0.0012 | 0.0016 | 0.0032 | 0.0007 | 0.0005 | 0.0011 | 0.0023 | 0.0029 | 0.0012 | 0.0005 | 0.0013 | 0.0008 | 0.0014 | 0.002 |
Bud Periphery | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0051 | 0 | 0.0001 | 0.001 | 0 | 0 |
Bud Site | 0.0002 | 0.0006 | 0.0005 | 0.0001 | 0.0002 | 0 | 0.0028 | 0.0014 | 0.0003 | 0.0002 | 0.0006 | 0.0001 | 0.0039 | 0.0005 | 0.0005 | 0.0017 | 0.0002 | 0.0001 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0 |
Cytoplasm | 0.2045 | 0.2905 | 0.2297 | 0.2293 | 0.3425 | 0.1855 | 0.2018 | 0.2817 | 0.2302 | 0.3307 | 0.2775 | 0.2185 | 0.2893 | 0.3842 | 0.3249 | 0.3852 | 0.4073 | 0.3062 |
Cytoplasmic Foci | 0.0014 | 0.0013 | 0.0019 | 0.0007 | 0.0023 | 0.0005 | 0.0085 | 0.001 | 0.001 | 0.0069 | 0.0064 | 0.0013 | 0.0141 | 0.0006 | 0.0008 | 0.002 | 0.0054 | 0.0013 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0135 | 0.0067 | 0.0075 | 0.0126 | 0.0256 | 0.0109 | 0.0182 | 0.0094 | 0.0124 | 0.0357 | 0.0171 | 0.0187 | 0.0218 | 0.012 | 0.0136 | 0.0086 | 0.0059 | 0.0118 |
Endosome | 0.0069 | 0.0056 | 0.0047 | 0.0033 | 0.0115 | 0.0033 | 0.0208 | 0.003 | 0.0055 | 0.0163 | 0.027 | 0.0077 | 0.0288 | 0.0015 | 0.0019 | 0.0119 | 0.0049 | 0.007 |
Golgi | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0033 | 0.0001 | 0.0002 | 0.0003 | 0.0049 | 0.0002 | 0.0077 | 0 | 0.0001 | 0.0016 | 0.0001 | 0.0002 |
Lipid Particles | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.005 | 0.0001 | 0.0002 | 0.0001 | 0.0195 | 0.0001 | 0.0093 | 0 | 0.0001 | 0.0008 | 0 | 0.001 |
Mitochondria | 0.0004 | 0.0003 | 0.0005 | 0.0003 | 0.0007 | 0.0003 | 0.0035 | 0.0003 | 0.0006 | 0.0009 | 0.006 | 0.0008 | 0.0049 | 0.0003 | 0.0003 | 0.0055 | 0.0005 | 0.0003 |
None | 0.0108 | 0.0014 | 0.0061 | 0.0007 | 0.0056 | 0.0005 | 0.0042 | 0.0014 | 0.0014 | 0.0027 | 0.0023 | 0.0014 | 0.0119 | 0.002 | 0.0006 | 0.0025 | 0.0013 | 0.0013 |
Nuclear Periphery | 0.1087 | 0.0637 | 0.0715 | 0.0935 | 0.0878 | 0.0713 | 0.1077 | 0.0591 | 0.0767 | 0.1028 | 0.1092 | 0.0798 | 0.1187 | 0.0702 | 0.0807 | 0.0464 | 0.0242 | 0.0602 |
Nucleolus | 0.0006 | 0.0007 | 0.0008 | 0.0005 | 0.0005 | 0.0005 | 0.0011 | 0.0006 | 0.0005 | 0.0005 | 0.0018 | 0.0006 | 0.001 | 0.0003 | 0.0005 | 0.0008 | 0.0003 | 0.0005 |
Nucleus | 0.6407 | 0.6192 | 0.6647 | 0.649 | 0.507 | 0.7191 | 0.5744 | 0.6331 | 0.662 | 0.487 | 0.4849 | 0.6563 | 0.4304 | 0.5221 | 0.5533 | 0.5096 | 0.5388 | 0.5988 |
Peroxisomes | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0067 | 0 | 0 | 0.0002 | 0.0065 | 0.0001 | 0.0071 | 0 | 0.0001 | 0.0005 | 0.0001 | 0 |
Punctate Nuclear | 0.0043 | 0.0048 | 0.0054 | 0.0042 | 0.0055 | 0.0027 | 0.0124 | 0.0042 | 0.0038 | 0.0093 | 0.0152 | 0.0056 | 0.0097 | 0.0025 | 0.0059 | 0.0043 | 0.0072 | 0.0058 |
Vacuole | 0.0057 | 0.0036 | 0.0028 | 0.0033 | 0.0072 | 0.0028 | 0.0049 | 0.0025 | 0.0028 | 0.003 | 0.0041 | 0.0034 | 0.0056 | 0.0021 | 0.002 | 0.0032 | 0.0017 | 0.0026 |
Vacuole Periphery | 0.0012 | 0.0007 | 0.0007 | 0.0009 | 0.0016 | 0.0008 | 0.0015 | 0.0005 | 0.0008 | 0.0011 | 0.0015 | 0.0011 | 0.0019 | 0.0004 | 0.0005 | 0.0018 | 0.0003 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 27.5376 | 16.4113 | 26.8754 | 31.4512 | 20.9298 | 34.1657 | 30.1829 | 34.3708 | 36.6889 | 28.3047 |
Translational Efficiency | 1.2932 | 2.0927 | 1.238 | 1.236 | 1.4944 | 1.3467 | 1.494 | 1.2777 | 1.2309 | 1.2601 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
600 | 1072 | 214 | 415 | 1230 | 1426 | 1317 | 400 | 1830 | 2498 | 1531 | 815 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 687.61 | 917.75 | 1374.21 | 1377.89 | 965.26 | 1006.97 | 1190.98 | 1535.77 | 874.23 | 968.68 | 1216.59 | 1455.38 |
Standard Deviation | 120.65 | 165.33 | 161.92 | 343.71 | 131.72 | 154.71 | 173.81 | 295.12 | 182.82 | 165.36 | 183.55 | 330.35 |
Intensity Change Log 2 | — | 0.416511 | 0.998940 | 1.002798 | — | 0.061031 | 0.303160 | 0.669973 | — | 0.219675 | 0.634092 | 0.817859 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000341 | 0.000815 | 0.003105 | 0.002737 | 0.000349 | 0.000517 | 0.000906 | 0.002375 | 0.000346 | 0.000645 | 0.001214 | 0.002559 |
Bud Neck | 0.002601 | 0.004651 | 0.018800 | 0.039056 | 0.002111 | 0.004566 | 0.008399 | 0.041982 | 0.002271 | 0.004603 | 0.009853 | 0.040492 |
Bud Site | 0.003100 | 0.007240 | 0.008557 | 0.021089 | 0.001937 | 0.006126 | 0.003536 | 0.018379 | 0.002319 | 0.006604 | 0.004238 | 0.019759 |
Cell Periphery | 0.000369 | 0.000189 | 0.000786 | 0.001617 | 0.000159 | 0.000127 | 0.000324 | 0.002086 | 0.000228 | 0.000154 | 0.000388 | 0.001847 |
Cytoplasm | 0.366188* | 0.494235* | 0.105289* | 0.224075* | 0.456199* | 0.423557* | 0.165991* | 0.149125* | 0.426687* | 0.453888* | 0.157506* | 0.187290* |
Cytoplasmic Foci | 0.059474 | 0.051436 | 0.006481 | 0.007697 | 0.038395 | 0.042130 | 0.003036 | 0.006380 | 0.045306 | 0.046124 | 0.003518 | 0.007051 |
Eisosomes | 0.000077 | 0.000031 | 0.000066 | 0.000054 | 0.000028 | 0.000030 | 0.000032 | 0.000049 | 0.000044 | 0.000030 | 0.000037 | 0.000052 |
Endoplasmic Reticulum | 0.013963 | 0.043188 | 0.051912 | 0.100568* | 0.070092 | 0.046375 | 0.031386 | 0.089939 | 0.051689 | 0.045007 | 0.034255 | 0.095351 |
Endosome | 0.004292 | 0.007588 | 0.010195 | 0.013247 | 0.005459 | 0.008022 | 0.003461 | 0.019180 | 0.005076 | 0.007836 | 0.004402 | 0.016159 |
Golgi | 0.000910 | 0.002026 | 0.000505 | 0.006763 | 0.001124 | 0.002767 | 0.000132 | 0.006352 | 0.001054 | 0.002449 | 0.000184 | 0.006561 |
Lipid Particles | 0.010311 | 0.007058 | 0.004494 | 0.011474 | 0.007360 | 0.007856 | 0.001571 | 0.015882 | 0.008327 | 0.007513 | 0.001980 | 0.013638 |
Mitochondria | 0.001810 | 0.002501 | 0.002583 | 0.008062 | 0.001744 | 0.006906 | 0.002321 | 0.005808 | 0.001766 | 0.005016 | 0.002358 | 0.006955 |
Mitotic Spindle | 0.000332 | 0.006012 | 0.002068 | 0.043417 | 0.003693 | 0.018697 | 0.006253 | 0.072236 | 0.002591 | 0.013254 | 0.005668 | 0.057561 |
None | 0.032750 | 0.009409 | 0.002070 | 0.005740 | 0.003962 | 0.003539 | 0.005842 | 0.004468 | 0.013400 | 0.006058 | 0.005315 | 0.005116 |
Nuclear Periphery | 0.005376 | 0.018861 | 0.024591 | 0.044974 | 0.023791 | 0.030170 | 0.022072 | 0.095944 | 0.017754 | 0.025316 | 0.022424 | 0.069990 |
Nuclear Periphery Foci | 0.015068 | 0.039948 | 0.018838 | 0.096775 | 0.034943 | 0.033842 | 0.009848 | 0.144626 | 0.028427 | 0.036462 | 0.011105 | 0.120260 |
Nucleolus | 0.008160 | 0.003251 | 0.001664 | 0.001529 | 0.001277 | 0.002399 | 0.001389 | 0.001750 | 0.003534 | 0.002765 | 0.001428 | 0.001637 |
Nucleus | 0.453821* | 0.253500* | 0.627947* | 0.286983* | 0.315762* | 0.314435* | 0.691697* | 0.255177* | 0.361027* | 0.288285* | 0.682787* | 0.271373* |
Peroxisomes | 0.001818 | 0.001039 | 0.002267 | 0.000248 | 0.000428 | 0.001918 | 0.000465 | 0.000199 | 0.000884 | 0.001541 | 0.000717 | 0.000224 |
Vacuole | 0.018258 | 0.044460 | 0.107246 | 0.082703 | 0.030459 | 0.043872 | 0.041062 | 0.066176 | 0.026459 | 0.044124 | 0.050313 | 0.074592 |
Vacuole Periphery | 0.000983 | 0.002562 | 0.000537 | 0.001192 | 0.000727 | 0.002151 | 0.000276 | 0.001888 | 0.000811 | 0.002327 | 0.000312 | 0.001534 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.81 | -11.52 | -9.02 | -6.93 | 1.20 | -2.69 | -8.93 | -3.29 | -3.07 | -2.14 | -4.64 | -12.93 | -6.63 | -5.79 | -3.55 |
Bud Neck | -2.07 | -9.23 | -13.54 | -12.96 | -7.13 | -5.88 | -13.69 | -14.34 | -13.71 | -11.98 | -5.05 | -15.53 | -19.82 | -18.89 | -15.64 |
Bud Site | -3.03 | -5.39 | -7.32 | -5.79 | -4.77 | -4.45 | -5.71 | -8.17 | -6.08 | -6.96 | -5.60 | -6.29 | -11.03 | -8.38 | -9.31 |
Cell Periphery | 3.21 | -6.03 | -6.47 | -7.45 | -4.21 | 3.39 | -8.14 | -4.80 | -4.87 | -4.46 | 3.75 | -6.52 | -7.33 | -7.62 | -6.66 |
Cytoplasm | -9.02 | 19.82 | 12.40 | 23.91 | -7.59 | 3.56 | 36.32 | 31.68 | 29.12 | 1.69 | -2.94 | 39.09 | 31.05 | 36.00 | -3.00 |
Cytoplasmic Foci | 1.86 | 12.39 | 12.31 | 13.96 | -1.37 | -1.17 | 15.96 | 13.79 | 15.43 | -5.47 | -0.03 | 20.38 | 18.41 | 20.76 | -6.58 |
Eisosomes | 7.48 | -0.30 | 1.14 | -5.70 | 1.49 | -0.79 | -5.52 | -5.03 | -4.77 | -3.51 | 5.22 | -0.68 | -4.39 | -7.29 | -4.23 |
Endoplasmic Reticulum | -10.05 | -7.38 | -8.68 | -4.37 | -3.72 | 5.35 | 9.73 | 1.06 | -2.76 | -5.48 | 2.25 | 6.31 | -3.30 | -5.06 | -7.71 |
Endosome | -4.89 | -4.25 | -7.03 | -3.52 | -1.14 | -3.97 | 4.08 | -7.87 | -5.48 | -9.42 | -5.86 | 1.52 | -10.26 | -6.39 | -10.96 |
Golgi | -2.43 | 2.87 | -1.15 | -0.58 | -1.37 | -2.73 | 4.73 | -1.08 | -0.18 | -1.64 | -3.48 | 5.92 | -1.55 | -0.50 | -2.21 |
Lipid Particles | 2.43 | 3.71 | -1.76 | -3.99 | -4.99 | -0.83 | 9.05 | -5.84 | -5.52 | -8.15 | 1.03 | 9.94 | -6.07 | -6.72 | -10.37 |
Mitochondria | -1.27 | -2.40 | -2.39 | -2.02 | -1.87 | -4.53 | -2.56 | -4.53 | 1.19 | -3.69 | -4.68 | -3.27 | -4.03 | -0.60 | -3.17 |
Mitotic Spindle | -4.96 | -4.12 | -6.20 | -5.23 | -5.63 | -5.43 | -2.53 | -7.18 | -5.54 | -6.59 | -6.23 | -3.41 | -9.24 | -7.29 | -8.10 |
None | 5.34 | 7.31 | 6.41 | 2.94 | -5.07 | 0.53 | -1.02 | -0.33 | -0.85 | 0.60 | 4.65 | 5.76 | 5.52 | 1.57 | -0.59 |
Nuclear Periphery | -6.70 | -3.91 | -7.84 | -3.81 | -2.97 | -2.13 | 0.82 | -6.65 | -5.73 | -7.02 | -3.75 | -2.04 | -8.45 | -6.55 | -7.47 |
Nuclear Periphery Foci | -6.70 | -1.08 | -8.62 | -4.76 | -7.29 | 0.25 | 9.46 | -8.46 | -8.53 | -11.58 | -3.25 | 8.11 | -11.23 | -9.63 | -14.56 |
Nucleolus | 2.51 | 3.82 | 2.93 | 0.99 | -1.12 | -1.62 | -1.04 | -2.18 | -0.22 | -1.80 | 0.78 | 3.20 | 1.18 | 0.49 | -1.95 |
Nucleus | 13.78 | -7.42 | 6.87 | -4.80 | 12.91 | 0.11 | -38.91 | 2.32 | 2.26 | 27.34 | 8.95 | -35.42 | 4.92 | -1.46 | 32.83 |
Peroxisomes | 2.26 | 0.63 | 4.46 | 6.01 | 1.28 | -4.24 | 0.11 | 4.07 | 4.83 | 1.15 | -2.57 | 1.74 | 5.26 | 6.24 | 1.64 |
Vacuole | -8.61 | -11.19 | -13.50 | -9.55 | 1.51 | -5.38 | -8.74 | -11.17 | -9.00 | -6.96 | -8.87 | -13.59 | -16.99 | -13.14 | -7.73 |
Vacuole Periphery | -2.65 | 1.16 | 0.51 | 3.76 | -1.45 | -4.31 | 5.66 | -1.19 | 2.18 | -2.62 | -5.06 | 3.67 | -0.61 | 4.26 | -3.54 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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