Standard name
Human Ortholog
Description Chaperone required for correct maturation of the 20S proteasome; short-lived chaperone; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.9 0.99 0.97 0.96 0.89 0.81 0.79 0.72 0.74 0.69 0.7 0.98 0.99 1.0 0.96 0.97 0.94 0.89 0.9 0.68 0.84 0.84 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.19 0 0.12 0.06 0.29 0.35 0.3 0.45 0.43 0.49 0.45 0 0 0 0.06 0 0 0 0 0.15 0.06 0.05 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 1 1 0 3 2
Bud 0 0 0 1 4 2 9 20 12 19 16 19 0 0 0 1 0 0 3 0 0 0 5 3
Bud Neck 0 0 3 2 6 1 10 13 1 14 3 9 0 0 0 3 0 0 1 0 0 0 0 2
Bud Site 0 0 0 0 0 2 4 2 1 1 1 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 1 1 0 0 1 0 1 1 1 2 0 1 0 4 1 1 1 0 0 0 0 1
Cytoplasm 379 77 92 290 345 337 350 510 316 387 239 376 301 428 488 151 70 93 333 83 52 104 276 333
Endoplasmic Reticulum 1 0 0 0 0 1 0 1 1 0 1 0 1 1 0 7 7 6 0 0 1 0 1 1
Endosome 0 0 0 0 0 0 0 1 0 0 0 0 2 0 0 1 1 1 6 0 0 0 3 5
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 1 2 0 0 0 2 1 0 0 0 2 0
Mitochondria 12 16 2 36 22 108 151 194 197 225 170 243 1 2 1 10 2 4 10 2 11 7 16 22
Nucleus 0 0 0 0 2 1 1 5 3 4 2 1 0 1 0 3 1 2 0 0 0 0 1 0
Nuclear Periphery 1 1 0 0 0 0 1 0 0 1 2 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1 0 0 0 0 2 1 1 2 0 1 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 1 2 18 29 6 15 6 14 0 0 0 0 0 0 3 0 3 0 3 10
Vac/Vac Membrane 0 1 0 0 0 1 1 0 3 1 0 5 0 0 0 2 0 1 3 1 1 3 6 6
Unique Cell Count 388 86 93 299 358 377 434 644 440 526 347 540 306 433 489 157 72 99 375 93 78 124 330 401
Labelled Cell Count 396 97 98 331 380 456 548 776 542 671 441 674 306 435 489 183 83 110 375 93 78 124 330 401


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 3.6 2.6 2.8 3.3 2.7 2.6 2.8 2.4 2.6 2.5 2.3 4.1 4.3 4.0 5.2 5.2 4.9 3.4 4.1 4.2
Std Deviation (1e-4) 0.6 0.7 0.6 0.9 1.3 1.2 1.2 1.1 1.2 1.4 1.5 0.6 1.5 1.4 1.1 1.6 1.0 1.3 0.7 1.4 1.3
Intensity Change (Log2) – – – 0.08 0.32 0.04 -0.01 0.06 -0.14 -0.0 -0.1 -0.21 0.62 0.69 0.61 0.97 0.97 0.88 0.36 0.64 0.65


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.5395 -1.0833 -1.148 -0.9663 -1.0445 -1.19 -1.3507 -1.4489 -1.2261 -1.3156 -0.881 -1.5126 -0.5129 0.3857 0.1021 0.1109 -0.4501 0.0888
Actin 0.0197 0.0042 0.0048 0.0805 0.0395 0.0002 0.0583 0.0008 0.0169 0.0259 0.031 0.0062 0.0126 0.0001 0.0014 0 0.0001 0.0028
Bud 0.0006 0.0006 0.0012 0.0089 0.0003 0 0.0018 0.0004 0.0012 0.0004 0.0033 0.0001 0 0.0001 0.0007 0 0 0.0005
Bud Neck 0.0008 0.0001 0.0005 0.0041 0.0018 0 0.0022 0.0001 0.0019 0.0003 0.0014 0.0006 0 0.0001 0.0007 0 0 0.0002
Bud Periphery 0.001 0.0006 0.0023 0.0338 0.0003 0 0.0036 0.0005 0.0024 0.0008 0.008 0.0002 0.0001 0.0001 0.0014 0 0 0.0012
Bud Site 0.0018 0.0048 0.0082 0.012 0.0012 0 0.0077 0.0016 0.0083 0.0011 0.0068 0.0033 0.0001 0.0036 0.0033 0 0 0.0001
Cell Periphery 0.0001 0.0001 0.0003 0.0002 0.0001 0 0.0004 0.0001 0.0006 0.0001 0.0002 0 0 0.0001 0.0001 0 0 0
Cytoplasm 0.0168 0.0149 0.0221 0.008 0.0066 0.0243 0.0245 0.0421 0.0328 0.0347 0.0372 0.0584 0.0198 0.0248 0.0243 0.0266 0.0136 0.0324
Cytoplasmic Foci 0.0281 0.0086 0.0104 0.029 0.0228 0.0023 0.0237 0.0113 0.0148 0.0036 0.037 0.005 0.0014 0.0012 0.0024 0.0007 0.0005 0.0029
Eisosomes 0.0004 0.0003 0.0005 0.0006 0.0002 0.0001 0.0009 0.0003 0.0008 0.0002 0.0001 0.0001 0.0002 0.0002 0.0002 0 0.0001 0.0001
Endoplasmic Reticulum 0.0018 0.0006 0.0008 0.0004 0.0003 0.0003 0.0019 0.002 0.0015 0.002 0.0021 0.0005 0.0002 0.0002 0.0007 0.0001 0 0.0003
Endosome 0.0104 0.002 0.0023 0.0054 0.0069 0.0001 0.0115 0.0022 0.0142 0.0197 0.0315 0.0042 0.0004 0.0001 0.0006 0.0001 0 0.0004
Golgi 0.0031 0.0016 0.0009 0.0037 0.0124 0 0.0074 0.0002 0.0092 0.0107 0.0191 0.0017 0.001 0 0.0001 0 0 0.0001
Lipid Particles 0.0115 0.0018 0.0039 0.0072 0.0435 0.0001 0.0136 0.001 0.008 0.0025 0.0098 0.0025 0.001 0.0001 0.0003 0 0 0.0004
Mitochondria 0.0028 0.0012 0.0013 0.0421 0.0065 0 0.0224 0.0003 0.0123 0.06 0.0074 0.002 0.0003 0.0001 0.0004 0.0001 0 0.0004
None 0.879 0.9487 0.9295 0.7238 0.8481 0.9719 0.7696 0.9332 0.8532 0.8142 0.7551 0.9072 0.9604 0.9689 0.9596 0.9719 0.9855 0.957
Nuclear Periphery 0.0034 0.0067 0.0011 0.0015 0.0002 0.0001 0.0029 0.0011 0.0048 0.0031 0.0038 0.0031 0.0003 0.0001 0.0006 0.0001 0 0.0002
Nucleolus 0.0004 0.0001 0.0005 0.0009 0.0004 0 0.0016 0.0001 0.0007 0.0001 0.0002 0.0004 0.0002 0.0001 0.0004 0 0 0.0001
Nucleus 0.0006 0.0004 0.0009 0.0011 0.0001 0.0001 0.0022 0.0005 0.0016 0.0003 0.0008 0.0005 0.0002 0.0001 0.0008 0.0001 0 0.0003
Peroxisomes 0.0106 0.0007 0.0055 0.0143 0.0067 0 0.0216 0.0006 0.0093 0.0166 0.0254 0.0017 0.0005 0 0.0002 0 0 0.0002
Punctate Nuclear 0.0055 0.0015 0.002 0.0208 0.0009 0.0002 0.0197 0.0008 0.0019 0.0003 0.0016 0.0017 0.001 0.0001 0.0012 0.0001 0 0.0003
Vacuole 0.0009 0.0001 0.0009 0.001 0.0005 0 0.0016 0.0007 0.002 0.0012 0.0094 0.0003 0.0001 0.0001 0.0004 0.0001 0 0.0001
Vacuole Periphery 0.0007 0.0003 0.0003 0.0007 0.0006 0 0.001 0.0001 0.0014 0.0023 0.0088 0.0003 0 0 0.0001 0 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 83.9279 52.9598 86.7841 93.9196 67.21 81.4143 89.246 94.1172 78.9985 83.8598
Translational Efficiency 2.905 3.9433 2.4671 2.0334 2.9866 3.5073 2.439 3.3155 3.4873 2.5071

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1678 1036 144 1049 1715 1421 1577 1761 3393 2457 1721 2810

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 621.11 683.42 902.95 893.87 607.61 706.41 808.13 805.89 614.29 696.72 816.06 838.73
Standard Deviation 71.35 91.00 105.61 197.35 73.87 93.32 90.52 99.44 72.94 93.04 95.56 150.16
Intensity Change Log 2 — 0.137924 0.539797 0.525216 — 0.217360 0.411442 0.407437 — 0.177752 0.477751 0.468175

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000090 0.000339 0.000731 0.015624 0.000077 0.000658 0.000262 0.000664 0.000084 0.000524 0.000301 0.006249
Bud Neck 0.016950 0.044989 0.001812 0.017774 0.016366 0.063195 0.005851 0.009799 0.016655 0.055519 0.005513 0.012776
Bud Site 0.003261 0.014380 0.013004 0.054426 0.001822 0.014911 0.015883 0.022087 0.002534 0.014687 0.015642 0.034159
Cell Periphery 0.000114 0.000136 0.000250 0.006531 0.000121 0.000453 0.000146 0.000143 0.000118 0.000320 0.000155 0.002528
Cytoplasm 0.483782* 0.348540* 0.221186* 0.273250* 0.419370* 0.277973* 0.369094* 0.396212* 0.451224* 0.307728* 0.356718* 0.350309*
Cytoplasmic Foci 0.221660* 0.290192* 0.016525 0.034331 0.239801* 0.265658* 0.011342 0.015419 0.230830* 0.276003* 0.011776 0.022479
Eisosomes 0.000168 0.000145 0.000100 0.001096 0.000198 0.000250 0.000036 0.000034 0.000184 0.000206 0.000042 0.000430
Endoplasmic Reticulum 0.000502 0.000659 0.008308 0.174507* 0.000800 0.000632 0.001405 0.001556 0.000653 0.000643 0.001983 0.066120
Endosome 0.009331 0.039352 0.003578 0.015160 0.008879 0.056353 0.002438 0.003418 0.009102 0.049184 0.002534 0.007801
Golgi 0.001066 0.008853 0.000109 0.006292 0.001639 0.008095 0.000263 0.000757 0.001355 0.008415 0.000250 0.002823
Lipid Particles 0.011548 0.008569 0.003339 0.051131 0.016407 0.017935 0.001130 0.000834 0.014004 0.013986 0.001315 0.019611
Mitochondria 0.003921 0.019711 0.000396 0.001735 0.009740 0.032559 0.002187 0.002149 0.006862 0.027142 0.002037 0.001995
Mitotic Spindle 0.001431 0.006582 0.000179 0.021814 0.001497 0.008202 0.012386 0.014870 0.001465 0.007519 0.011365 0.017462
None 0.003641 0.002326 0.003205 0.001979 0.003228 0.002323 0.004517 0.009211 0.003432 0.002324 0.004407 0.006511
Nuclear Periphery 0.000528 0.000572 0.001674 0.001209 0.000784 0.000628 0.001605 0.001170 0.000658 0.000604 0.001611 0.001185
Nuclear Periphery Foci 0.000280 0.000397 0.002114 0.009661 0.000213 0.000223 0.000398 0.000359 0.000246 0.000296 0.000542 0.003832
Nucleolus 0.001152 0.003085 0.000772 0.000427 0.001860 0.003040 0.000524 0.000533 0.001510 0.003059 0.000544 0.000494
Nucleus 0.192045* 0.110339 0.640841* 0.145658* 0.233680* 0.119577 0.523584* 0.469176* 0.213090* 0.115682 0.533395* 0.348404*
Peroxisomes 0.003854 0.023689 0.000428 0.003189 0.008571 0.023441 0.001109 0.001259 0.006238 0.023545 0.001052 0.001980
Vacuole 0.043947 0.073262 0.081225 0.163007 0.034080 0.099647 0.044930 0.049435 0.038960 0.088521 0.047967 0.091832
Vacuole Periphery 0.000729 0.003884 0.000221 0.001198 0.000865 0.004246 0.000910 0.000913 0.000797 0.004094 0.000852 0.001019

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.46 -7.17 -5.72 -5.56 -5.20 -1.55 -5.49 -1.95 0.39 -1.06 -2.04 -6.90 -5.95 -4.97 -5.57
Bud Neck -8.21 11.25 2.01 9.21 -7.73 -12.70 7.50 5.18 15.52 -2.76 -15.10 10.54 5.54 18.08 -5.25
Bud Site -7.08 -0.93 -10.25 -6.54 -4.00 -7.05 -5.42 -7.14 -0.30 -1.62 -9.57 -5.07 -12.10 -4.41 -5.23
Cell Periphery -1.74 -6.03 -6.58 -6.55 -6.27 -1.95 -1.11 0.28 1.96 1.22 -2.04 -2.81 -6.50 -5.02 -6.35
Cytoplasm 12.73 15.00 20.85 6.03 -4.30 15.63 11.50 9.02 -6.31 -2.28 20.79 19.20 20.29 -0.95 -1.15
Cytoplasmic Foci -7.69 38.49 38.13 35.55 -3.71 -3.33 44.31 43.58 43.22 -1.93 -7.72 61.34 58.68 56.05 -6.17
Eisosomes 1.93 2.50 -3.63 -3.82 -3.91 -3.15 14.92 15.70 16.80 2.79 -1.95 18.22 -1.67 -1.26 -4.30
Endoplasmic Reticulum -2.25 -10.34 -13.27 -13.24 -11.73 1.10 -8.46 -8.33 -12.60 -0.25 0.07 -14.64 -13.00 -13.00 -12.15
Endosome -11.67 5.95 -0.55 11.13 -5.46 -15.45 8.45 7.55 17.88 -0.19 -19.19 11.44 5.82 21.19 -4.95
Golgi -7.32 10.89 -4.35 4.35 -6.07 -7.90 7.69 4.98 9.42 -1.31 -10.86 10.02 -0.85 9.97 -5.74
Lipid Particles 2.48 7.08 -5.96 -7.03 -8.60 -0.86 12.96 13.31 13.26 4.57 0.06 16.35 1.45 1.37 -8.91
Mitochondria -7.17 5.38 3.30 8.59 -2.78 -7.36 5.54 5.56 11.26 -0.06 -10.17 6.34 6.44 13.98 -0.15
Mitotic Spindle -3.42 2.02 -5.69 -3.81 -6.17 -4.29 -4.33 -6.01 -1.98 -1.18 -5.51 -4.41 -8.31 -4.10 -2.79
None 2.43 1.85 2.11 -0.13 -0.14 1.36 -1.50 -4.59 -5.63 -3.77 2.52 -1.12 -3.45 -5.44 -2.50
Nuclear Periphery -0.41 -11.34 -12.55 -8.51 0.30 1.36 -10.90 -9.19 -14.93 2.57 0.60 -17.11 -15.94 -15.74 2.34
Nuclear Periphery Foci -1.48 -7.45 -7.93 -7.79 -4.19 -0.84 -6.00 -7.06 -6.06 0.95 -1.44 -7.07 -8.26 -8.08 -6.64
Nucleolus -4.06 3.26 4.89 5.55 2.52 -2.80 5.39 5.52 7.84 -0.32 -5.09 6.40 7.21 9.34 0.19
Nucleus 12.34 -17.89 -2.02 -10.48 16.48 17.30 -37.19 -30.24 -43.99 5.05 20.88 -45.79 -26.10 -39.66 18.06
Peroxisomes -10.18 9.96 3.14 10.96 -2.93 -8.41 9.71 9.40 13.86 -0.01 -13.30 11.60 9.99 17.73 -1.40
Vacuole -7.78 -6.23 -23.74 -18.79 -6.28 -16.08 -10.58 -13.99 2.15 -3.41 -17.22 -10.95 -25.38 -11.69 -13.30
Vacuole Periphery -5.18 4.82 -0.16 5.01 -3.17 -9.23 0.91 1.24 9.19 0.11 -9.99 0.91 1.01 9.85 -0.23
Standard name
Human Ortholog
Description Chaperone required for correct maturation of the 20S proteasome; short-lived chaperone; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Ump1

Ump1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ump1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available