Standard name
Human Ortholog
Description Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0
Bud 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0
Bud Neck 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0
Bud Site 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0
Cytoplasm 0.05 0.05 – 0.07 0.13 0.09 – 0.07 0.07 0.07 0.08 0.06 0.15 0.19 0.23 0.32 0.34 0.31 0 0 – 0 0 0
Endoplasmic Reticulum 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 – 0 0 0
Endosome 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0.09 0.05 0 0 0 – 0 0 0
Golgi 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0
Mitochondria 0.12 0 – 0.47 0.24 0.65 – 0.56 0.76 0.68 0.71 0.78 0 0 0 0.08 0.08 0 0 0 – 0 0 0
Nucleus 0.89 0.91 – 0.74 0.67 0.58 – 0.62 0.58 0.59 0.57 0.53 0.82 0.75 0.7 0.32 0.41 0.5 0.83 0.85 – 0.64 0.57 0.51
Nuclear Periphery 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0
Nucleolus 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0
Peroxisomes 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0
SpindlePole 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0
Vac/Vac Membrane 0 0.05 – 0 0.1 0.06 – 0 0 0 0 0.05 0 0.06 0.07 0.26 0.22 0.17 0.1 0.08 – 0.23 0.25 0.32
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 2 – 0 0 1
Bud 0 0 – 0 0 2 – 11 6 11 9 5 0 1 0 0 1 0 5 2 – 4 2 6
Bud Neck 1 0 – 0 0 0 – 1 0 2 0 0 0 1 1 0 1 0 0 0 – 1 1 1
Bud Site 0 0 – 0 1 8 – 9 10 17 12 19 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 1 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 – 0 0 0
Cytoplasm 44 16 – 26 57 41 – 49 38 43 39 33 82 167 222 68 41 39 11 1 – 9 8 10
Endoplasmic Reticulum 2 3 – 0 1 0 – 0 0 0 0 0 0 1 1 11 3 8 0 1 – 1 2 3
Endosome 2 0 – 0 9 1 – 1 0 0 0 0 2 2 24 20 6 3 7 2 – 11 10 9
Golgi 0 0 – 0 0 0 – 0 0 0 0 0 0 1 2 1 0 0 0 1 – 2 5 11
Mitochondria 108 11 – 169 105 292 – 382 429 444 353 411 8 9 8 17 10 3 10 4 – 8 15 14
Nucleus 817 289 – 266 291 261 – 425 327 390 285 280 457 660 688 67 49 64 811 283 – 295 278 278
Nuclear Periphery 1 0 – 3 2 2 – 1 4 3 4 3 0 3 3 0 0 0 0 0 – 1 2 3
Nucleolus 0 0 – 3 0 2 – 8 6 7 3 3 1 3 4 3 0 0 3 0 – 7 19 13
Peroxisomes 0 0 – 0 0 0 – 0 0 0 0 0 0 0 2 0 0 0 0 0 – 0 0 0
SpindlePole 4 0 – 0 0 2 – 1 1 1 2 1 1 1 9 2 0 1 16 0 – 8 8 10
Vac/Vac Membrane 35 17 – 12 42 28 – 29 22 23 19 27 23 55 66 55 26 22 96 26 – 105 123 177
Unique Cell Count 917 318 360 433 448 685 562 657 498 525 558 882 986 212 120 127 972 332 464 486 550
Labelled Cell Count 1014 336 479 509 639 917 843 941 726 782 574 904 1031 244 137 140 972 332 464 486 550


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 5.5 – 3.9 4.3 3.5 – 3.8 3.2 3.5 3.4 3.3 5.2 5.5 5.3 5.7 6.5 5.9 5.4 5.6 5.6
Std Deviation (1e-4) 0.6 1.2 – 1.6 1.9 1.2 – 1.4 0.8 1.1 1.2 1.1 1.2 1.3 1.2 1.7 2.7 2.1 1.5 1.5 1.5
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2485 1.4598 1.0773 1.2499 1.1845 1.4007 0.5888 0.4694 0.3044 0.5854 0.4634 0.326 2.1642 2.0733 1.8956 1.7648 1.23 1.7928
Actin 0.0089 0 0.009 0.0143 0.0043 0.0018 0.0203 0.0001 0.0155 0.0067 0.0056 0.0018 0.0333 0 0.0196 0.0055 0.0153 0.0013
Bud 0.0001 0 0.0001 0.0001 0.0007 0 0.0006 0.0002 0.0005 0.0008 0.0002 0.0001 0.0003 0 0.0001 0.0001 0.0002 0.0001
Bud Neck 0.0008 0.0001 0.0011 0.0234 0.0039 0.0009 0.0019 0.0003 0.0012 0.001 0.001 0.0049 0.0175 0.0002 0.0013 0.0011 0.0012 0.0015
Bud Periphery 0.0002 0 0.0001 0.0002 0.0027 0.0001 0.0014 0.0002 0.0015 0.0029 0.0002 0.0002 0.0006 0 0.0002 0.0001 0.0008 0.0004
Bud Site 0.0002 0.0001 0.0015 0.0009 0.0017 0.0001 0.002 0.0006 0.0028 0.0017 0.0006 0.0002 0.0033 0.0003 0.0007 0.0004 0.0003 0.0001
Cell Periphery 0.0001 0 0.0001 0.0002 0.0003 0 0.0002 0 0.0003 0.0006 0.0001 0.0001 0.0004 0 0.0001 0 0.0001 0
Cytoplasm 0.0031 0.0041 0.0006 0.0003 0.0014 0.0006 0.0093 0.0053 0.0018 0.0011 0.0289 0.0034 0.013 0.0021 0.0037 0.0042 0.0164 0.001
Cytoplasmic Foci 0.0007 0.0001 0.0002 0.0001 0.0004 0.0003 0.0116 0.0007 0.0051 0.004 0.0093 0.0005 0.0029 0 0.003 0.0005 0.0014 0.0002
Eisosomes 0.0002 0 0.0002 0.0004 0.0001 0 0.0003 0 0.0001 0.0002 0.0001 0 0.0007 0 0.0002 0.0001 0.0002 0
Endoplasmic Reticulum 0.001 0.0001 0.0007 0.0002 0.0009 0.0002 0.0031 0.0003 0.0004 0.0009 0.0035 0.001 0.0032 0.0001 0.0013 0.0016 0.0027 0.0004
Endosome 0.004 0.0001 0.0015 0.0007 0.0038 0.0018 0.0244 0.0008 0.0074 0.0163 0.0116 0.0033 0.0133 0.0002 0.0048 0.0033 0.0132 0.002
Golgi 0.0008 0 0.0006 0.0008 0.0007 0.0006 0.0055 0.0001 0.0063 0.0113 0.0204 0.0011 0.003 0 0.0013 0.0003 0.0044 0.0005
Lipid Particles 0.001 0 0.0003 0.0002 0.0011 0.0011 0.0092 0.0021 0.0131 0.0723 0.0118 0.0016 0.0032 0 0.0046 0.0003 0.0074 0.0006
Mitochondria 0.0011 0.0001 0.0006 0.0003 0.0041 0.0004 0.0305 0.0013 0.0053 0.0412 0.0129 0.0071 0.003 0.0002 0.0006 0.0007 0.0031 0.001
None 0.0075 0.0034 0.0004 0.0002 0.0013 0.0002 0.0289 0.0065 0.0017 0.0012 0.0034 0.0026 0.0177 0.0022 0.012 0.0106 0.0052 0.0007
Nuclear Periphery 0.0145 0.0036 0.0099 0.0015 0.0186 0.007 0.0146 0.0093 0.0098 0.0058 0.0076 0.0083 0.0227 0.0081 0.0145 0.0254 0.0457 0.0071
Nucleolus 0.0111 0.0104 0.0091 0.0107 0.0226 0.0197 0.0103 0.0147 0.012 0.0048 0.0118 0.0166 0.0121 0.0125 0.0169 0.011 0.0071 0.024
Nucleus 0.9296 0.9664 0.9513 0.9402 0.9063 0.9475 0.8009 0.947 0.8952 0.7902 0.8407 0.9277 0.8158 0.9635 0.8969 0.8721 0.8538 0.9483
Peroxisomes 0.0005 0 0.0001 0.0001 0.0007 0.0002 0.0047 0.0014 0.0065 0.014 0.0242 0.0009 0.0021 0 0.0054 0.0001 0.0009 0.0001
Punctate Nuclear 0.0131 0.0112 0.0123 0.0048 0.0207 0.0171 0.0136 0.0081 0.0114 0.0148 0.0045 0.0176 0.0278 0.0101 0.0113 0.0615 0.0174 0.0097
Vacuole 0.0008 0.0001 0.0003 0.0001 0.0023 0.0002 0.0039 0.0007 0.0015 0.0051 0.0011 0.0006 0.0029 0.0003 0.0013 0.0005 0.0013 0.0005
Vacuole Periphery 0.0007 0 0.0002 0.0001 0.0014 0.0002 0.0028 0.0002 0.0006 0.0031 0.0005 0.0004 0.001 0.0001 0.0003 0.0005 0.0021 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.3138 18.5608 7.8703 11.1922 7.6915 2.9645 16.0859 24.428 13.6957 12.1425
Translational Efficiency 0.8457 0.9227 2.1738 1.6209 1.7691 6.8649 1.038 0.8909 1.6203 1.413

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1700 216 320 254 797 995 2246 1184 2497 1211 2566 1438

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 643.30 815.69 897.84 925.15 698.80 753.03 822.34 825.05 661.01 764.21 831.76 842.73
Standard Deviation 90.21 99.11 110.06 117.12 80.78 94.49 104.33 128.08 91.06 98.30 107.98 131.86
Intensity Change Log 2 — 0.342529 0.480967 0.524196 — 0.107828 0.234855 0.239602 — 0.225096 0.358067 0.383028

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000042 0.000990 0.000602 0.000868 0.000105 0.000188 0.000285 0.000709 0.000062 0.000331 0.000325 0.000737
Bud Neck 0.002328 0.005079 0.004850 0.008593 0.001175 0.003365 0.015969 0.019483 0.001960 0.003671 0.014582 0.017560
Bud Site 0.000458 0.001915 0.001842 0.002078 0.000332 0.001528 0.003439 0.008987 0.000418 0.001597 0.003240 0.007767
Cell Periphery 0.000165 0.000168 0.000045 0.000149 0.000121 0.000122 0.000121 0.000273 0.000151 0.000130 0.000112 0.000251
Cytoplasm 0.100892* 0.030606 0.002315 0.022819 0.041822 0.037130 0.007604 0.037896 0.082038 0.035967 0.006944 0.035233
Cytoplasmic Foci 0.017224 0.015688 0.000086 0.000156 0.013242 0.009424 0.000284 0.002987 0.015953 0.010542 0.000259 0.002487
Eisosomes 0.000028 0.000069 0.000027 0.000035 0.000036 0.000027 0.000017 0.000025 0.000030 0.000035 0.000018 0.000027
Endoplasmic Reticulum 0.001045 0.008743 0.008365 0.009481 0.001791 0.002686 0.005020 0.006791 0.001283 0.003766 0.005437 0.007266
Endosome 0.000764 0.001389 0.000267 0.000637 0.000680 0.001092 0.000568 0.005295 0.000737 0.001145 0.000530 0.004472
Golgi 0.000420 0.002146 0.000037 0.000091 0.001182 0.000642 0.000333 0.002336 0.000663 0.000911 0.000296 0.001940
Lipid Particles 0.003441 0.005157 0.000250 0.000380 0.003113 0.002284 0.000377 0.000956 0.003336 0.002796 0.000361 0.000855
Mitochondria 0.003365 0.008405 0.000631 0.001081 0.003552 0.005995 0.001641 0.006995 0.003425 0.006425 0.001515 0.005950
Mitotic Spindle 0.000807 0.010355 0.002186 0.013086 0.000357 0.002402 0.004333 0.017097 0.000664 0.003821 0.004065 0.016389
None 0.009062 0.003429 0.001100 0.012617 0.005246 0.006552 0.001775 0.007739 0.007844 0.005995 0.001691 0.008600
Nuclear Periphery 0.001763 0.001367 0.000493 0.001303 0.000920 0.002353 0.001056 0.002282 0.001494 0.002177 0.000986 0.002109
Nuclear Periphery Foci 0.000805 0.000621 0.000121 0.000390 0.000186 0.000378 0.000336 0.000570 0.000607 0.000422 0.000309 0.000538
Nucleolus 0.003540 0.003765 0.001675 0.003358 0.003812 0.003969 0.001256 0.001948 0.003627 0.003933 0.001308 0.002197
Nucleus 0.850191* 0.884499* 0.970858* 0.912618* 0.918213* 0.913129* 0.950326* 0.861232* 0.871903* 0.908022* 0.952887* 0.870309*
Peroxisomes 0.001483 0.006950 0.000190 0.000150 0.001240 0.003164 0.000172 0.000635 0.001405 0.003839 0.000175 0.000549
Vacuole 0.001960 0.008220 0.003961 0.009932 0.002672 0.002854 0.004480 0.014225 0.002187 0.003811 0.004415 0.013466
Vacuole Periphery 0.000218 0.000439 0.000100 0.000180 0.000202 0.000715 0.000608 0.001538 0.000213 0.000666 0.000545 0.001298

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.41 -7.40 -12.90 -0.27 -3.82 -1.82 -5.58 -8.92 -7.76 -6.65 -4.57 -12.52 -12.51 -6.36 -7.76
Bud Neck -3.18 -4.55 -7.24 -4.71 -4.89 -2.99 -10.99 -10.13 -8.64 -1.83 -2.99 -10.95 -11.01 -9.41 -2.28
Bud Site -2.95 -1.73 -5.25 -1.44 -1.19 -1.74 -5.11 -6.81 -4.92 -3.71 -2.19 -5.24 -7.31 -5.08 -3.80
Cell Periphery -0.16 4.95 1.20 1.04 -3.77 -0.03 0.51 -1.08 -1.07 -1.27 0.87 2.38 -0.64 -0.96 -1.45
Cytoplasm 10.62 23.17 14.42 1.73 -4.26 1.11 9.77 2.63 1.56 -8.58 11.32 24.18 14.04 2.03 -9.34
Cytoplasmic Foci 0.45 12.26 12.15 3.73 -2.07 1.55 7.59 6.51 5.61 -3.42 3.04 14.30 12.57 6.73 -3.45
Eisosomes -4.43 0.14 -4.05 2.65 -3.86 2.14 6.17 1.72 -0.87 -7.52 -1.91 7.38 -1.29 1.07 -8.71
Endoplasmic Reticulum -6.78 -9.64 -11.02 -1.58 -2.48 -2.58 -9.68 -12.52 -11.17 -6.01 -8.32 -17.43 -18.16 -10.12 -6.87
Endosome -1.72 3.22 -0.22 1.66 -4.14 -1.32 1.37 -3.23 -2.66 -3.54 -1.56 2.29 -3.17 -2.49 -3.67
Golgi -1.28 2.44 1.80 1.53 -5.49 0.75 1.43 -0.23 -1.10 -1.83 -0.70 1.66 -0.92 -0.42 -1.84
Lipid Particles -1.05 7.82 7.20 3.15 -2.82 1.27 5.53 4.53 3.79 -1.48 1.04 8.43 7.18 4.91 -1.59
Mitochondria -1.12 2.97 1.96 1.60 -6.43 -1.17 1.30 -0.97 0.30 -2.72 -1.73 2.34 -0.96 0.89 -2.81
Mitotic Spindle -1.60 -1.03 -3.02 -1.17 -2.50 -2.07 -3.46 -5.77 -4.73 -3.81 -2.48 -3.57 -6.49 -4.56 -4.49
None 4.06 10.78 0.21 -2.58 -4.48 -1.09 4.27 -0.74 0.49 -5.64 1.80 10.60 1.59 -0.43 -6.95
Nuclear Periphery 1.48 6.41 -1.88 -2.47 -3.99 -1.83 -3.51 -5.15 -0.40 -3.87 -1.07 1.62 -3.88 -0.77 -4.85
Nuclear Periphery Foci 0.32 1.52 0.26 -0.15 -3.54 -1.80 -0.64 -4.15 -0.26 -1.82 0.23 1.14 0.09 -0.28 -2.36
Nucleolus -0.26 6.12 -0.93 -0.54 -3.41 -0.28 2.93 2.03 3.57 -1.86 -0.70 5.88 2.74 3.08 -3.07
Nucleus -1.95 -16.21 -2.93 -0.27 7.04 0.61 -4.81 5.10 4.50 10.85 -4.96 -16.11 -0.40 4.01 12.35
Peroxisomes -2.37 5.38 5.48 3.01 0.35 -2.19 3.76 2.38 3.12 -1.81 -2.90 6.52 4.38 4.14 -1.79
Vacuole -3.85 -2.68 -4.95 -2.55 -4.36 0.33 -1.96 -7.78 -8.08 -6.90 -3.29 -4.30 -9.89 -8.30 -8.05
Vacuole Periphery -1.47 2.60 0.57 1.69 -1.88 -1.85 -1.85 -3.29 -0.75 -1.62 -1.98 -1.70 -3.22 -0.60 -1.55
Standard name
Human Ortholog
Description Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress
Localization
Cell Percentages nucleus (81%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Aim4

Aim4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aim4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available